Podcasts about bhpd

This episode follows Keyes biggest challenge yet, living with his old BHPD partner, Paul Went. After getting near the point of a mental breakdown, Keyes agrees to go out for a night on the town with all the guys, which leads to a questionably sober disaster of an evening. Pop the Tylenol and mix yourself a bloody mary, because you're sure to feel this one in the morning. Enjoy this week's episode of Dopple Avenue Hurt. In loving memory of Patricia Vivien.  www.sharkdropper.com @sharkdropper www.langcomedy.com  

Well, now Keyes has gone and done it. It looks like all the meddling around in the O'Reilly case, all the going behind the BHPD's business landed Ol' Jimmy in the slammer. He better watch himseld though, because he may just run into a familiar... friend. After being bailed out, James receivines an interesting oppurtunity to go meet with famous actress, Julianne Goode, in the flesh, at her luxurious mansion. She wines and dines him, and then drops a proprosal in his lap that lays the ground work for the entire case. Listen and find out if James Keyes accepts or respectfully declines, in this week's episode of Dopple Avenue Hurt. www.sharkdropper.com www.langcomedy.com @sharkdropper Plase rate and subscribe to us on iTunes and please visit sharkdropper.com for more fictional podcasts like this one. 

In this week's episode of Dopple Avenue Hurt, Keyes races to the hospital to discover the shocking truth of why Paul, his old partner from the BHPD, was rushed to the hospital. Lorraine and Arthur join him at Paul's bedside to help discover new leads in The Case of the Silver Casket, and take the first real offensive approach against solving this doozy. Could this be the moment where our favorite P.I. finally figures it all out? Perhaps. Listen, and find out.   www.sharkdropper.com @sharkdropper

The two coupling agents SPDP (N-succinimidyl-3-(2-pyridyldithio)propionate) and SATA (N-succinimidyl-S-acetylthioacetate) were compared in their efficiency and feasibility to couple monoclonal antibodies (Abs) via thioether linkage to liposomes functionalized by various lipophilic maleimide compounds like Full-size image methyl ester (MP-PL), N-(3-maleimidopropionyl)phosphatidylethanolamide (MP-PE), Full-size image methyl ester (EMC-PL), and N-(6-maleimidocaproyl)phosphatidylethanolamine (EMC-PE). The composition of the liposomes was soy phosphatidylcholine (SPC), cholesterol, maleimide compounds and -tocopherol (1:0.2:0.02:0.01, mol parts), plus N4-oleylcytosine arabinoside (NOAC) as cytostatic prodrug (0.2 mol parts) and a new, lipophilic and highly fluorescent dye N,N′-bis(1-hexylheptyl)-3,4:9,10-perylenebis(dicarboximide) (BHPD, 0.006 mol parts). From the maleimide derivatives MP-PL was the most effective in terms of preservation of the coupling activity in dependence of liposome storage. The coupling of the monoclonal A B8-24.3 (mouse IgG2b, MHC class I, anti H-2kb) and IB16-6 (rat IgG2a, anti B16 mouse melanoma) to the drug carrying liposomes was more effective and easier to accomplish with SATA as compared to SPDP. Coupling rates of 60–65% were obtained with SATA at molar ratios of 12 SATA:1 Ab:40 maleimide spacer groups on the surface of one liposome. The highest coupling rates with SPDP were obtained at the ratio of 24 SPDP:1 Ab:40 liposomal maleimide groups, with an Ab binding efficiency of only 20–25%. The optimal in vitro binding conditions to specific target cells (EL4 for B8-24.3-liposomes and B16-F10 for IB16-6-liposomes) were determined by cytofluorometric measurement of the liposomal BHPD fluorescence with SATA linked Abs. Optimal immunoliposome binding to specific epitopes on the target cells was achieved with 1–2 Ab molecules coupled to one liposome, with immunoliposome concentrations of 20–130 nM and with a small incubation volume of 0.3–0.4 ml. The specificity of the binding of B8-24.3-liposomes to EL4 target cells was visualized by scanning electron microscopy. Antibody mediated endocytic uptake of immunoliposomes could be demonstrated by transmission electron microscopy.