Podcasts about electron microscopy

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Play Episode Listen Later Jul 28, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.26.550598v1?rss=1 Authors: Dorkenwald, S., Schneider-Mizell, C. M., Brittain, D., Halageri, A., Jordan, C., Kemnitz, N., Castro, M. A., Silversmith, W., Maitin-Shephard, J., Troidl, J., Pfister, H., Gillet, V., Xenes, D., Bae, J. A., Bodor, A. L., Buchanan, J., Bumbarger, D. J., Elabbady, L., Jia, Z., Kapner, D., Kinn, S., Lee, K., Li, K., Lu, R., Macrina, T., Mahalingam, G., Mitchell, E., Mondal, S. S., Mu, S., Nehoran, B., Popovych, S., Takeno, M., Torres, R., Turner, N. L., Wong, W., Wu, J., Yin, W., Yu, S.-c., Reid, R. C., da Costa, N. M., Seung, H. S., Collman, F. Abstract: Advances in Electron Microscopy, image segmentation and computational infrastructure have given rise to large-scale and richly annotated connectomic datasets which are increasingly shared across communities. To enable collaboration, users need to be able to concurrently create new annotations and correct errors in the automated segmentation by proofreading. In large datasets, every proofreading edit relabels cell identities of millions of voxels and thousands of annotations like synapses. For analysis, users require immediate and reproducible access to this constantly changing and expanding data landscape. Here, we present the Connectome Annotation Versioning Engine (CAVE), a computational infrastructure for immediate and reproducible connectome analysis in up-to petascale datasets (~1mm3) while proofreading and annotating is ongoing. For segmentation, CAVE provides a distributed proofreading infrastructure for continuous versioning of large reconstructions. Annotations in CAVE are defined by locations such that they can be quickly assigned to the underlying segment which enables fast analysis queries of CAVE's data for arbitrary time points. CAVE supports schematized, extensible annotations, so that researchers can readily design novel annotation types. CAVE is already used for many connectomics datasets, including the largest datasets available to date. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

152. The Electron Microscopy Episode: Meet the BEST Electron Microscopist in the WORLD!

I'M THE VILLAIN

Play Episode Listen Later Jul 26, 2023 50:11

Ok that might be a bit of an exaggeration, Cosmo Alto may not be the electron microscopist in the world, or North America, or Manhattan, but he is definitely the best electron microscopist I know. In this episode, Cosmo sits down with us and tells us about the wonderful world of electron microscopy. If you're not familiar, for the majority of scientific history, we were using light microscopes to look at small things, which essentially just utilizes lenses to magnify things to the size where you can see them, like the microscope you probably used in science class. An ELECTRON microscope is used to look at things which are smaller than the wavelength of light! So the things you can look at in an electron microscope are on the picometer scale, which means you can look at the structure of proteins from the brain, for example. The microscope shoots a laser at the proteins and an electron detector receives the electrons that bounce off the protein, forming an image that scientists can then look at and synthesize into their research. Lastly, I always like to ask guests who talk about technology how hard it would be to turn the technology they work on into a weapon: listen on to hear about whether you could turn an electron microscope into a space laser! Music is The Beauty of Maths by Meydän. --- Support this podcast: https://podcasters.spotify.com/pod/show/im-the-villain/support

EMDiffuse: a diffusion-based deep learning method augmenting ultrastructural imaging and volume electron microscopy

Play Episode Listen Later Jul 12, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.12.548636v1?rss=1 Authors: Lu, C., Chen, K., Qiu, H., Chen, X., Chen, G., Qi, X., Jiang, H. Abstract: Electron microscopy (EM) revolutionized the way to visualize cellular ultrastructure. Volume EM (vEM) has further broadened its three-dimensional nanoscale imaging capacity. However, intrinsic trade-offs between imaging speed and quality of EM restrict the attainable imaging area and volume. Isotropic imaging with vEM for large biological volumes remains unachievable. Here we developed EMDiffuse, a suite of algorithms designed to enhance EM and vEM capabilities, leveraging the cutting-edge image generation diffusion model. EMDiffuse demonstrates outstanding denoising and super-resolution performance, generates realistic predictions without unwarranted smoothness, improves prediction resolution by ~30%, and exhibits excellent transferability by taking only one pair of images to fine-tune. EMDiffuse also pioneers the isotropic vEM reconstruction task, generating isotropic volume similar to that obtained using advanced FIB-SEM even in the absence of isotropic training data. We demonstrated the robustness of EMDiffuse by generating isotropic volumes from six public datasets obtained from different vEM techniques and instruments. The generated isotropic volume enables accurate organelle reconstruction, making 3D nanoscale ultrastructure analysis faster and more accessible and extending such capability to larger volumes. More importantly, EMDiffuse features self-assessment functionalities and guarantees reliable predictions for all tasks. We envision EMDiffuse to pave the way for more in-depth investigations into the intricate subcellular nanoscale structures within large areas and volumes of biological systems. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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ATUM-Tomo: A multi-scale approach to cellular ultrastructure by combined volume scanning electron microscopy and electron tomography

Play Episode Listen Later Jul 12, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.12.547497v1?rss=1 Authors: Kislinger, G., Fabig, G., Wehn, A. C., Rodriguez, L., Jiang, H., Niemann, C., Klymchenko, A. S., Plesnila, N., Misgeld, T., Mueller-Reichert, T., Khalin, I., Schifferer, M. Abstract: Like other volume electron microscopy approaches, Automated Tape Collecting Ultramicrotomy (ATUM) enables imaging of serial sections deposited on thick plastic tapes by scanning electron microscopy (SEM). However, ATUM is unique by enabling hierarchical imaging and thus efficient screening for target structures as needed e.g., for correlated light and electron microscopy. However, SEM of sections on tape can only access the section surface, thereby limiting the axial resolution to the typical size of cellular vesicles, an order of magnitude lower than the acquired xy resolution. In contrast, serial-section electron tomography (ET), a transmission electron microscopy-based approach, yields isotropic voxels at full EM resolution, but requires deposition of sections on electron-permeant thin and fragile monolayer films - thus making screening of large section libraries difficult and prone to section loss. To combine the strength of both approaches, we developed ATUM-Tomo, a hybrid method, where sections are first reversibly attached to plastic tape via a dissolvable coating, and after screening detached and transferred to the ET-compatible thin films. Thus, ATUM-SEM of serial semi-thick sections and consecutive ET of one selected section combines SEM's fast target recognition and coarse rendering capability with ET's high-resolution volume visualizations - thus enabling multi-scale interrogation of cellular ultrastructure. As a proof-of-principle, we applied correlative ATUM-Tomo to study ultrastructural features of blood brain barrier (BBB) leakiness around microthrombi in a mouse model of traumatic brain injury. Microthrombi and associated sites of BBB leakiness were identified by confocal imaging of injected fluorescent and electron-dense nanoparticles, then relocalized by ATUM-SEM, and finally interrogated by correlated ATUM-Tomo, a workflow which created a seamless zoom-in on structural BBB pathology from the micro- to the nanometer scale. Overall, our new ATUM-Tomo approach will substantially advance ultrastructural analysis of biological phenomena that require cell- and tissue-level contextualization of the finest subcellular textures. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Resolving exit strategies of mycobacteria by combining high-pressure freezing with 3D-correlative light and electron microscopy

3d chemistry physics materials 2d appalachian hudak electron microscopy

Play Episode Listen Later Apr 24, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.24.538041v1?rss=1 Authors: Franzkoch, R., Anand, A., Breitsprecher, L., Psathaki, O. E., Barisch, C. Abstract: The infection course of Mycobacterium tuberculosis is highly dynamic and comprises sequential stages that require damaging and crossing of several membranes to enable the translocation of the bacteria into the cytosol or their escape from the host. Many important breakthroughs such as the restriction of vacuolar and cytosolic mycobacteria by the autophagy pathway and the recruitment of sophisticated host repair machineries to the Mycobacterium-containing vacuole have been gained in the Dictyostelium discoideum/M. marinum system. Despite the availability of well-established light and advanced electron microscopy techniques in this system, a correlative approach that integrates both methodologies with almost native ultrastructural preservation is still lacking at the moment. This is most likely due to the low ability of D. discoideum to adhere to surfaces, which results in cell loss even after fixation. To address this problem, we improved the adhesion of cells and developed a straightforward and convenient workflow for 3D-correlative light and electron microscopy. This approach includes high-pressure freezing, which is an excellent technique for preserving membranes. Thus, our method allows to monitor the ultrastructural aspects of vacuole escape which is of central importance for the survival and dissemination of bacterial pathogens. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Dr. Bethany Hudak on Physics , Chemistry, Electron Microscopy, and 2D materials

Kourosh Khoylou Podcast

Play Episode Listen Later Mar 3, 2023 52:03

Dr. Bethany Hudak is a Research Chemist. In this episode, Bathany and I talk about her childhood in the Appalachian mountains, her brothers, her love for science,quantum physics, electron microscopy, Grad Schools, 2D and 3D materials.Support the show

Scanning electron microscopy of human islet cilia

Play Episode Listen Later Feb 15, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.15.528685v1?rss=1 Authors: Polino, A. J., Sviben, S., Melena, I., Piston, D. W., HUGHES, J. Abstract: Human islet primary cilia are vital glucose-regulating organelles whose structure remains uncharacterized. Scanning electron microscopy (SEM) is a useful technique for studying the surface morphology of membrane projections like primary cilia, but conventional sample preparation does not reveal the sub-membrane axonemal structure which holds key implications for cilia function. To overcome this challenge, we combined SEM with membrane-extraction techniques to examine cilia in native human islets. Our data show well-preserved cilia subdomains which demonstrate both expected and unexpected ultrastructural motifs. Morphometric features were quantified when possible, including axonemal length and diameter, microtubule conformations and chirality. We further describe a novel ciliary ring, a structure that may be a specialization in human islets. Key findings are correlated with fluorescence microscopy and interpreted in the context of cilia function as a cellular sensor and communications locus in pancreatic islets. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Cryo-FIB workflow for imaging brain tissue via in situ cryo-electron microscopy

MRS Bulletin Materials News Podcast

Play Episode Listen Later Feb 12, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.11.528064v1?rss=1 Authors: Ning, J., Glausier, J. R., Hsieh, C., Schmelzer, T., Buck, S. A., Franks, J., Hampton, C. M., Lewis, D. A., Marko, M., Freyberg, Z. Abstract: Cryo-electron microscopy (cryo-EM) enables the study of protein complexes, cytoskeletal elements, and organelles in three dimensions without the use of chemical fixation. Most cryo-EM studies focus on vitreously frozen individual cells separated from their native tissue contexts. This reliance on imaging of single cells is primarily due to technical challenges associated with preparing fresh tissue sections at a thinness sufficient for visualization via cryo-EM. Highly heterogenous and specialized tissues, such as brain, are especially affected by this limitation as the cellular, subcellular, and synaptic milieus can significantly vary across neuroanatomical locations. To address this limitation, we established new instrumentation and a workflow that consists of: 1) high-pressure freezing of fresh brain tissue; 2) tissue trimming followed by cryo-focused ion beam milling via the H-bar approach to generate ultrathin lamellae; and 3) cryo-EM imaging. Here, we apply this workflow to visualize the fine ultrastructural details of organelles, as well as cytoskeletal and synaptic elements that comprise the cortical neuropil within fresh, unfixed mouse brain tissue. Moreover, we present initial studies that apply principles of the above workflow to the analysis of postmortem human brain tissue. Overall, our work integrates the strengths of cryo-electron microscopy and tissue-based approaches to produce a generalizable workflow capable of visualizing subcellular structures within complex tissue environments. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Episode 2: Real-time 3D imaging software for electron microscopy developed

Play Episode Play 52 sec Highlight Listen Later Feb 1, 2023 3:24 Transcription Available

In this podcast episode, MRS Bulletin's Laura Leay interviews Robert Hovden from the University of Michigan and his graduate student Jonathan Schwartz on development of the freely available Tomviz platform (tomviz.org) that enables real-time three-dimensional (3D) visual analysis of materials. Building on the already existing Tomviz platform, Schwartz created new algorithms capable of pulling data from transmission and scanning electron microscopes, evolving the 3D image as the experiment progresses. This research is published in a recent issue of Nature Communications (https://doi.org/10.1038/s41467-022-32046-0).

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Targeted volume Correlative Light and Electron Microscopy of an environmental marine microorganism

Play Episode Listen Later Jan 28, 2023

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.27.525698v1?rss=1 Authors: Mocaer, K., Mizzon, G., Gunkel, M., Halavatyi, A., Steyer, A. M., Oorschot, V., Schorb, M., Le Kieffre, C., Yee, D. P., Chevalier, F., Gallet, B., Decelle, J., Schwab, Y., Ronchi, P. Abstract: Photosynthetic microalgae are responsible for an important fraction of CO2 fixation and O2 production on Earth. Three-dimensional ultrastructural characterization of these organisms in their natural environment can contribute to a deeper understanding of their cell biology. However, the low throughput of volume electron microscopy (vEM) methods, along with the complexity and heterogeneity of environmental samples, pose great technical challenges. In the present study, we used a workflow based on a specific EM sample preparation, compatible with both light and vEM imaging in order to target one cell among a complex natural community. This method revealed the 3D subcellular landscape of a photosynthetic dinoflagellate with quantitative characterization of multiple organelles. We could show that this cell contains a single convoluted chloroplast and the arrangement of the flagellar apparatus with its associated photosensitive elements. Moreover, we observed chromatin features that could shed light on how transcriptional activity takes place in organisms where chromosomes are permanently condensed. Together with providing insights in dinoflagellates biology, this proof-of-principle study illustrates an efficient tool for the targeted ultrastructural analysis of environmental microorganisms in heterogeneous mixes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy

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Play Episode Listen Later Nov 24, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.23.517701v1?rss=1 Authors: Zheng, Z., Own, C. S., Wanner, A. A., Koene, R. A., Hammerschmith, E. W., Silversmith, W. M., Kemnitz, N., Tank, D. W., Seung, H. S. Abstract: We have achieved a three fold increase in the speed of transmission electron microscopy by using a beam deflecting mechanism to enable highly efficient acquisition of multiple image tiles for each motion of the mechanical stage. For millimeter-scale areas, the duty cycle of imaging doubles and exceeds 30%, yielding a net average imaging rate of 0.3 gigapixels per second. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles

Play Episode Listen Later Nov 23, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.22.516823v1?rss=1 Authors: Redpath, G. M. I., Rae, J. A., Yao, Y., Ruan, J., Cagigas, M. L., Whan, R., Hardeman, E. C., Gunning, P. W., Ananthanarayanan, V., Parton, R. G., Ariotti, N. Abstract: Unambiguous targeting of cellular structures for in situ cryo-electron microscopy in the heterogeneous, dense, and compacted environment of the cytoplasm remains challenging. Here we have developed a novel cryogenic correlative light and electron microscopy (cryo-CLEM) workflow which combines thin cells grown on a mechanically defined substratum to rapidly analyse organelles and macromolecular complexes in the cell by cryo-electron tomography (cryo-ET). We coupled these advancements with optogenetics to redistribute perinuclear-localised organelles to the cell periphery for cryo-ET. This reliable and robust workflow allows for fast in situ analyses without the requirement for cryo-focused ion beam milling. We have developed a protocol where cells can be frozen, imaged by cryo-fluorescence microscopy and ready for batch cryo-ET within a day. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

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Extracting Mitochondrial Cristae Characteristics from 3D Focused Ion Beam Scanning Electron Microscopy Data

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Play Episode Listen Later Nov 9, 2022

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.08.515664v1?rss=1 Authors: Wang, C., Ostergaard, L., Hasselholt, S., Sporring, J. Abstract: Mitochondria are the main suppliers of energy for cells and their bioenergetic function is regulated by mitochondrial dynamics: the constant changes in mitochondria size, shape, and cristae morphology to secure cell homeostasis. Although mitochondrial dysfunction is implicated in a wide range of diseases, our understanding of mitochondrial function remains limited by the complexity of inferring these spatial features from 2D electron microscopical (EM) images of intact tissue. Here, we present a semi-automatic method for segmentation and 3D reconstruction of mitochondria, cristae, and intracristal spaces based on 2D EM images of the murine hippocampus. We show that our method provides a more accurate characterization of mitochondrial ultrastructure in 3D than common 2D approaches and propose an operational index of mitochondria's internal organization. We speculate that this tool may help increase our understanding of mitochondrial dynamics in health and disease. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

What Is Cryo-Electron Microscopy? A Brief Introduction

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Play Episode Listen Later Sep 15, 2022 16:27

#26 — Cryo-EM is a revolutionary imaging method that lets us see complex biostructures at higher and higher resolutions. But do you understand the mind-blowing science behind this technique? And what is cryo-electron microscopy, anyway? Why is the cryogenic aspect important, and how did it seemingly go from nothing to the big time? In the latest episode of Mentors At Your Benchside, we answer all of these questions and more! Check out the corresponding online article to access loads of follow-up resources to deepen your understanding of this topic.[1] Also, check out our related articles covering crucial sample preparation considerations for cryo-EM and its history from obscure to Nobel Prize winner. [2,3] Resources: 1. What Is Cryo-Electron Microscopy? Available at: https://bitesizebio.com/62871/what-is-cryo-electron-microscopy/ 2. Cryo-EM Sample Prep: 5 Crucial Considerations. Available at: https://bitesizebio.com/62619/cryo-em-sample-prep/ 3. A Short History of Cryo-Electron Microscopy: Available at: https://bitesizebio.com/62839/history-of-cryo-electron-microscopy/

A Short History of Cryo-Electron Microscopy

nobel prize fascinating short history brief introduction cryo electron microscopy

Play Episode Listen Later Jun 28, 2022 9:07

#3 — In this episode of Mentors At Your Benchside, listen to a short history of cryo-electron microscopy—the Nobel Prize-winning technique revolutionizing molecular and structural biology. The journey takes us from the inception of cryo-EM- obscure, inferior, and derided- to the present-day competition for access to the incredibly powerful Krios microscopes. It's all about the power of an image. And speaking of images, be sure to read A Short History of Cryo-Electron Microscopy for a graphical timeline and some stunning cryo-EM structures. [1] Fascinating, cross-disciplinary science underpins cryo-EM—the technique is as broad as it is popular. To expand your understanding of the fundamental science behind it, Read What Is Cryo-Electron Microscopy? A Brief Introduction. [2] For tips and tricks on getting your sample ready for a cryo-EM experiment, check out Cryo-EM Sample Prep: 5 Crucial Considerations. [3] And for a simple illustration of the stunning structures cryo-EM produces, browse the Electron Microscopy Data Bank. [4] Resources: 1. A Short History of Cryo-Electron Microscopy. Available at: https://bitesizebio.com/62839/history-of-cryo-electron-microscopy/ 2. What Is Cryo-Electron Microscopy? A Brief Introduction. Available at: https://bitesizebio.com/62871/what-is-cryo-electron-microscopy/ 3. Cryo-EM Sample Prep: 5 Crucial Considerations. Available at: https://bitesizebio.com/62619/cryo-em-sample-prep/ 4. Electron Microscopy Data Bank. Available at: https://www.ebi.ac.uk/emdb/

A Short History of Cryo-Electron Microscopy

nobel prize fascinating short history brief introduction cryo electron microscopy

Play Episode Listen Later Jun 28, 2022 9:07

#3 — In this episode of Mentors At Your Benchside, listen to a short history of cryo-electron microscopy—the Nobel Prize-winning technique revolutionizing molecular and structural biology. The journey takes us from the inception of cryo-EM- obscure, inferior, and derided- to the present-day competition for access to the incredibly powerful Krios microscopes. It's all about the power of an image. And speaking of images, be sure to read A Short History of Cryo-Electron Microscopy for a graphical timeline and some stunning cryo-EM structures. [1] Fascinating, cross-disciplinary science underpins cryo-EM—the technique is as broad as it is popular. To expand your understanding of the fundamental science behind it, Read What Is Cryo-Electron Microscopy? A Brief Introduction. [2] For tips and tricks on getting your sample ready for a cryo-EM experiment, check out Cryo-EM Sample Prep: 5 Crucial Considerations. [3] And for a simple illustration of the stunning structures cryo-EM produces, browse the Electron Microscopy Data Bank. [4] Resources: 1. A Short History of Cryo-Electron Microscopy. Available at: https://bitesizebio.com/62839/history-of-cryo-electron-microscopy/ 2. What Is Cryo-Electron Microscopy? A Brief Introduction. Available at: https://bitesizebio.com/62871/what-is-cryo-electron-microscopy/ 3. Cryo-EM Sample Prep: 5 Crucial Considerations. Available at: https://bitesizebio.com/62619/cryo-em-sample-prep/ 4. Electron Microscopy Data Bank. Available at: https://www.ebi.ac.uk/emdb/

Oxford Instruments (OXIG) Full Year 2022 results presentation - June 2022

piworld audio investor podcasts

Play Episode Listen Later Jun 14, 2022 30:14

Oxford Instruments Chief Executive, Ian Barkshire, and CFO, Gavin Hill present the Full Year 2022 results for the period ended 31 March 2022. Ian Barkshire, Chief Executive Introduction - 00:18 Highlights - 00:45 Horizon Strategy & Progress - 03:20 Group performance - End Markets - 05:30 Gavin Hill, CFO Financial review - 07:08 Ian Barkshire, Chief Executive End market overview - 19:12 Sustainability update - 26:29 Summary and outlook - 28:34 Oxford Instruments plc is a United Kingdom-based provider of high technology products and services to the various industrial companies and scientific research communities. The Company's businesses include Andor, Asylum Research, Imaris, Magnetic Resonance, Nano Analysis, NanoScience, Plasma Technology, WITec and X-Ray Technology. The Company's products include Atomic Force Microscopy; Electron Microscopy, Deposition & Etch Tools, Low Temperature Systems, Optical Imaging, Nuclear Magnetic Resonance, Modular Optical Spectroscopy, Raman Microscopy and X-Ray. The Company's applications include Advanced Manufacturing; Agriculture & Food; Astronomy; Automotive & Aerospace; Bio Imaging & Life Science; Chemical & Catalysis; Energy Generation & Storage; Forensics & Environment; Geology, Petrology & Mining; Metals, Alloys, Composites & Ceramics; Pharma; Photonics; Polymers; Quantum Technologies; and Semiconductors, Microelectronics and Data Storage.

Electron Microscopy & Unidentified ”Viral” Objects

Dr Sam Bailey

Play Episode Listen Later Feb 15, 2022 19:09

How many times do we get shown pictures that are said to depict "viruses"? While this seems to trick the majority of people, I know you guys will be suspicious and will want a deeper dive into these claims. Let's crack open the black box of Electron Microscopy and see what these UVO's really are!

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#Ep61. Scanning Electron Microscopy

A Material Point of View

Play Episode Listen Later Feb 10, 2022 13:12

I will do one analysis in a SEM, Scanning Electron Microscope.I had to watch one video about this and I want to share some knowledge about SEM and its detectors--Sponsorship via e-mail: amaterialpointofview@gmail.com--Don't forget to follow the Podcast on Instagram and share this episode with your friends:https://www.instagram.com/amaterialpointofview/

sem scanning electron microscopy

Article Summary by Kensei Taguchi, Craig R Brooks, and Bertha C Elias

Kidney360

Play Episode Listen Later Dec 30, 2021 9:20

This is an article summary of "Quantitative super-resolution microscopy reveals promoting mitochondrial interconnectivity protects against AKI" by Kensei Taguchi, Craig R Brooks, and Bertha C Elias on behalf of coauthors.

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Podcast 21: Interview with Eva Nogales: How Cryogenic Electron Microscopy Informs Molecular Biology

Lessons from Lab and Life

Play Episode Listen Later Feb 5, 2021 31:57

Listen to Dr. Eva Nogales describe how cryo-electron microscopy addresses the challenge of visualizing macromolecular structures.

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CHD7 interacts with the nucleosome acidic patch for its efficient activity via its N-terminal region

PaperPlayer biorxiv biochemistry

Play Episode Listen Later Nov 19, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.19.389429v1?rss=1 Authors: Lee, E., Kang, C., Purhonen, P., Hebert, H., Bouazoune, K., Hohng, S., Song, J.-J. Abstract: Chromodomain-Helicase DNA binding protein 7 (CHD7) is an ATP dependent chromatin remodeler involved in maintaining open chromatin structure. Mutations of CHD7 gene causes multiple developmental disorders, notably CHARGE syndrome. However, there is not much known about the molecular mechanism by which CHD7 remodels nucleosomes. Here, we performed integrative biophysical analysis on CHD7 chromatin remodeler using crosslinking-mass spectrometry (XL-MS), cryo-Electron Microscopy (cryo-EM) and single-molecule Forster Resonance Energy Transfer (smFRET). We uncover that N-terminal to the Chromodomain (N-CRD) interacts with nucleosome. Importantly, this region is required for efficient ATPase stimulation and nucleosome remodeling activity of CHD7. The cryo-EM analysis on the N-CRD_Chromodomain bound to nucleosome reveals that the N-CRD interacts with the acidic patch of nucleosome. Furthermore, smFRET analysis shows the mutations in the N-CRD result in slow or highly-fluctuating remodeling activity. Collectively, our results uncover the functional importance of a previously unidentified N-terminal region in CHD7 and implicate that the multiple domains in chromatin remodelers are involved in regulating their activities. Copy rights belong to original authors. Visit the link for more info

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A spotlight on electron microscopy, featuring Dr Giorgio Divitini

BlueSci Podcast

Play Episode Listen Later Nov 16, 2020 35:30

This week we speak to Dr Giorgio Divitini, the Advanced TEM Officer in the High Resolution Electron Microscopy Group in the Department of Materials Science & Metallurgy at the University of Cambridge. Giorgio tells us about the background, history and science of electron microscopy, as well as the incredible architectural processes that must take place before setting up an electron microscope facility. We also learn about the vast applications of electron microscopy, all the way from biology to engineering, and find out how this often overlooked technology is likely to evolve in the future. The image used for the promotional art was taken by Giorgio, and you can find more amazing electron micrographs on his Flickr site: https://www.flickr.com/photos/giorgiodivitini/albums/72157633723329562. The BlueSci Podcast is run by the Cambridge University Science Magazine, currently hosted by Ruby Coates and Simone Eizagirre and sponsored by Greiner Bio-One. Visit www.bluesci.co.uk to access our free magazine, and find out how to get involved. If you enjoyed this episode, please subscribe! We welcome your feedback and suggestions via email: podcast(at)bluesci.co.uk. You can also follow us on Twitter on @bluescipod.

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In situ structure determination using single particle cryo-electron microscopy images

PaperPlayer biorxiv biophysics

Play Episode Listen Later Sep 4, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.04.282509v1?rss=1 Authors: Cheng, J., Li, B., Si, L., Zhang, X. Abstract: Cryo-electron microscopy (cryo-EM) tomography is a powerful tool for in situ structure determination. However, this method requires the acquisition of tilt series, and its time consuming throughput of acquiring tilt series severely slows determination of in situ structures. By treating the electron densities of non-target protein as non-Gaussian distributed noise, we developed a new target function that greatly improves the efficiency of the recognition of the target protein in a single cryo-EM image without acquiring tilt series. Moreover, we developed a sorting function that effectively eliminates the model dependence and improved the resolution during the subsequent structure refinement procedure. By eliminating model bias, our method allows using homolog proteins as models to recognize the target protein. Together, we developed an in situ single particle analysis (isSPA) method. Our isSPA method was successfully applied to solve structures of glycoproteins on the surface of a non-icosahedral virus and Rubisco inside the carboxysome. The cryo-EM data from both samples were collected within 24 hours, thus allowing fast and simple structural determination in situ. Copy rights belong to original authors. Visit the link for more info

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Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments

PaperPlayer biorxiv biochemistry

Play Episode Listen Later Aug 26, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.26.268748v1?rss=1 Authors: Chou, S. Z., Pollard, T. D. Abstract: We report high resolution cryo-electron microscopy structures of actin filaments with N-1-pyrene conjugated to cysteine 374 and either ADP (3.2 [A]) or ADP-phosphate (3.0 [A]) in the active site. Polymerization buries pyrene in a hydrophobic cavity between subunits along the long-pitch helix with only minor differences in conformation compared with native actin filaments. These structures explain how polymerization increases the fluorescence 20-fold, how myosin and cofilin binding to filaments reduces the fluorescence and how profilin binding to actin monomers increases the fluorescence. Copy rights belong to original authors. Visit the link for more info

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CorRelator: An interactive and flexible toolkit for high-precision cryo-correlative light and electron microscopy

PaperPlayer biorxiv biophysics

Play Episode Listen Later Aug 6, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.06.240481v1?rss=1 Authors: Yang, J. E., Larson, M. R., Sibert, B. S., Shrum, S., Wright, E. R. Abstract: Cryo-correlative light and electron microscopy (CLEM) is a technique that uses the spatiotemporal cues from fluorescence light microscopy (FLM) to investigate the high-resolution ultrastructure of biological samples by cryo-electron microscopy (cryo-EM). Cryo-CLEM provides advantages for identifying and distinguishing fluorescently labeled proteins, macromolecular complexes, and organelles from the cellular environment. Challenges remain on how correlation workflows and software tools are implemented on different microscope platforms to support microscopy-driven structural studies. Here, we present an open-source desktop application tool, CorRelator, to bridge between cryo-FLM and cryo-EM/ET data collection instruments. CorRelator was designed to be flexible for both on-the-fly and post-acquisition correlation schemes. The CorRelator workflow is easily adapted to any fluorescence and transmission electron microscope (TEM) system configuration. CorRelator was benchmarked under cryogenic and ambient temperature conditions using several FLM and TEM instruments, demonstrating that CorRelator is a rapid and efficient application for image and position registration in CLEM studies. CorRelator is a cross-platform software featuring an intuitive Graphical User Interface (GUI) that guides the user through the correlation process. CorRelator source code is available at: https://github.com/wright-cemrc-projects/corr. Copy rights belong to original authors. Visit the link for more info

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A genetic probe for visualizing glutamatergic synapses and vesicles by 3D electron microscopy

director science research professor resolutions collaboration penn state life sciences biomedical engineering huck cryo assistant research professor resolution revolution electron microscopy jennifer gray

Play Episode Listen Later Aug 3, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.31.230995v1?rss=1 Authors: Steinkellner, T., Madany, M., Haberl, M. G., Zell, V., Li, C., Hu, J., Mackey, M., Ramachandra, R., Adams, S., Ellisman, M. H., Hnasko, T., BOASSA, D. Abstract: Communication between neurons relies on the release of diverse neurotransmitters, which represent a key-defining feature of a neuron's chemical and functional identity. Neurotransmitters are packaged into vesicles by specific vesicular transporters. However, tools for labeling and imaging synaptic vesicles based on their neurochemical identity remain limited. We developed a genetically encoded probe to identify glutamatergic synaptic vesicles at the levels of both light and electron microscopy (EM) by fusing the mini singlet oxygen generator (miniSOG) probe to an intra-lumenal loop of the vesicular glutamate transporter-2. We then used a 3D imaging method, serial block face scanning EM, combined with a deep learning approach for automatic segmentation of labeled synaptic vesicles to assess the subcellular distribution of transporter-defined vesicles at nanometer scale. These tools represent a new resource for accessing the subcellular structure and molecular machinery of neurotransmission and for transmitter-defined tracing of neuronal connectivity. Copy rights belong to original authors. Visit the link for more info

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Cryo-Electron Microscopy and the Resolution Revolution

The Symbiotic Podcast

Play Episode Listen Later Jul 23, 2020 61:38 Transcription Available

In this episode, recorded in-person on February 4, 2020, we talked to a team of Penn State researchers and about the stunning advancements made in recent years in the field of electron microscopy. Up for discussion was a short history of electron microscopy, including how the addition of cryogenics has enabled new materials and structures to be imaged, and how breakthroughs in certain scientific fields have enabled advances in some very different disciplines. We also talked about how Penn State has robustly supported the development of cryo-EM and leveraged its advantage to create a world-class instrumentation facility.Relevant Links:Kelly LabCryo-Electron Microscopy Facility at Penn StateGuests:Deb Kelly is Professor of Biomedical Engineering and Director of the Center for Structural Oncology at Penn State. Her research involves the innovative use of cryoelectron microscopy and in situ transmission electron microscopy to deeply investigate the tiniest of biological structures. Dr. Kelly's mission is to engineer new molecular paradigms to create a world without cancer.Jennifer Gray is an assistant research professor and staff scientist at Penn State's Materials Research Institute, specializing in transmission electron microscopy and cryoelectron microscopy. As a highly specialized technologist working at the convergence of materials and life sciences, Gray helps researchers from both camps to benefit from the breakthroughs made by the other. Cameron Varano is Assistant Research Professor of Biomedical Engineering at Penn State. Her research in structural biology involves prolific use of cryoelectron microscopy to examine and categorize exosomes involved in the development of metastatic cancer.

Cryo-electron Microscopy and Exploratory Antisense Targeting of the 28-kDa Frameshift Stimulation Element from the SARS-CoV-2 RNA Genome

PaperPlayer biorxiv biochemistry

Play Episode Listen Later Jul 20, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.18.209270v1?rss=1 Authors: Zhang, K., Zheludev, I. N., Hagey, R. J., Wu, M. T.-P., Haslecker, R., Hou, Y. J., Kretsch, R., Pintilie, G. D., Rangan, R., Kladwang, W., Li, S., Pham, E. A., Bernardin, C., Baric, R. S., Sheahan, T. P., Dsouza, V., Glenn, J. S., Chiu, W., Das, R. Abstract: Drug discovery campaigns against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) are beginning to target the viral RNA genome1, 2. The frameshift stimulation element (FSE) of the SARS-CoV-2 genome is required for balanced expression of essential viral proteins and is highly conserved, making it a potential candidate for antiviral targeting by small molecules and oligonucleotides3-6. To aid global efforts focusing on SARS-CoV-2 frameshifting, we report exploratory results from frameshifting and cellular replication experiments with locked nucleic acid (LNA) antisense oligonucleotides (ASOs), which support the FSE as a therapeutic target but highlight difficulties in achieving strong inactivation. To understand current limitations, we applied cryogenic electron microscopy (cryo-EM) and the Ribosolve7 pipeline to determine a three-dimensional structure of the SARS-CoV-2 FSE, validated through an RNA nanostructure tagging method. This is the smallest macromolecule (88 nt; 28 kDa) resolved by single-particle cryo-EM at subnanometer resolution to date. The tertiary structure model, defined to an estimated accuracy of 5.9 [A], presents a topologically complex fold in which the 5' end threads through a ring formed inside a three-stem pseudoknot. Our results suggest an updated model for SARS-CoV-2 frameshifting as well as binding sites that may be targeted by next generation ASOs and small molecules. Copy rights belong to original authors. Visit the link for more info

Neurotransmitter Classification from Electron Microscopy Images at Synaptic Sites in Drosophila

images copy sites bates classification gaba funke neurotransmitters drosophila synaptic biorxiv vns electron microscopy

Play Episode Listen Later Jun 13, 2020

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.12.148775v1?rss=1 Authors: Eckstein, N., Bates, A. S., Du, M., Hartenstein, V., Jefferis, G. S. X. E., Funke, J. Abstract: High-resolution electron microscopy (EM) of nervous systems enables the reconstruction of neural circuits at the level of individual synaptic connections. However, for invertebrates, such as Drosophila melanogaster, it has so far been unclear whether the phenotype of neurons or synapses alone is sufficient to predict specific functional properties such as neurotransmitter identity. Here, we show that in Drosophila melanogaster artificial convolutional neural networks can confidently predict the type of neurotransmitter released at a synaptic site from EM images alone. The network successfully discriminates between six types of neurotransmitters (GABA, glutamate, acetylcholine, serotonin, dopamine, and octopamine) with an average accuracy of 87% for individual synapses and 94% for entire neurons, assuming each neuron expresses only one neurotransmitter. This result is surprising as there are often no obvious cues in the EM images that human observers can use to predict neurotransmitter identity. We apply the proposed method to quantify whether, similar to the ventral nervous system (VNS), all hemilineages in the Drosophila melanogaster brain express only one fast acting transmitter within their neurons. To test this principle, we predict the neurotransmitter identity of all identified synapses in 89 hemilineages in the Drosophila melanogaster adult brain. While the majority of our predictions show homogeneity of fast-acting neurotransmitter identity within a single hemilineage, we identify a set of hemilineages that express two fast-acting neurotransmitters with high statistical significance. As a result, our predictions are inconsistent with the hypothesis that all neurons within a hemilineage express the same fast-acting neurotransmitter in the brain of Drosophila melanogaster. Copy rights belong to original authors. Visit the link for more info

Extracting Meaning From Big Data in Volume Electron Microscopy with Martin Jones

New Matter: Inside the Minds of SLAS Scientists

Play Episode Listen Later Apr 4, 2020 23:02

Martin Jones works in the Electron Microscopy Science Technology Platform at the Francis Crick Institute in London, developing new hardware and software for extracting meaning from ever more complex data sets. He and SLAS Scientific Director, Mike Tarselli, explore Extracting Meaning From Big Data in Volume Electron Microscopy.Registration is now open for the 2022 AI Data Pipelines for Life Sciences Symposium in Seattle, WA, September 26-27.This two-day symposium will allow participants to explore how AI data pipelines are integrated into the life sciences. Attendees will learn about MLOPS, applications, techniques, and architectures of data and their uses in the life sciences. The SLAS 2022 Bio Entrepreneurship Symposium will allow emerging bio entrepreneurs, start-up companies, academics and those considering bio-entrepreneurship to explore the start-up ecosystem. Register by visiting: https://www.slas.org/events-calendar/slas-2022-bio-entrepreneurship-symposium/attend/register/

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2. Electron microscopy, the mystery of calcification and dino DNA

Röntgen's Radio - A PEM podcast

Play Episode Listen Later Feb 25, 2020 42:12

Dr Sergio Bertazzo talks about his research in Mineralomics, the study of minerals that form in the body and how they relate to disease. He talks about how imaging these minerals with an electron microscope can give us new insights into the causes of some very common diseases. We also discuss his foray into the Jurassic World as he recalls the time he found dinosaur blood in a fossil. Learn more about the department and its degree programmes at: https://www.ucl.ac.uk/medical-physics-biomedical-engineering/

mystery jurassic world calcification dino dna electron microscopy

Podcast 21: Interview with Eva Nogales: How Cryogenic Electron Microscopy Informs Molecular Biology

Lessons from Lab and Life

Play Episode Listen Later Jan 29, 2020 31:56

Listen to Dr. Eva Nogales describe how cryo-electron microscopy addresses the challenge of visualizing macromolecular structures.

molecular biology informs cryogenics electron microscopy protein structure molecular structure eva nogales

Science, society and drug design

Eavesdrop on Experts

Play Episode Listen Later Nov 26, 2019 23:32

Now a professor of biochemistry at the University of Cambridge, in 1969 Sir Thomas Blundell was one of the first people to see what the hormone insulin looked like. As part of the team led by Nobel Prize winner Dorothy Hodgkin, it was a medical breakthrough for diabetes patients everywhere. “I was always interested in doing a range of different things,” Professor Blundell says. “I came from a family where my grandfather was a very gifted artist and musician. And although my parents left school when they were 14 and 15, they always encouraged me to think more broadly.” “So I may be a little bit unusual because I’ve ended up doing things in politics, music and science, and that of course led me to advise prime ministers and to run organisations and found companies.” Professor Blundell’s research has focussed on understanding the structure and function of molecules for targets to improve drug design. “By using X-rays with very short wavelength, I can see these very tiny molecules. Add in other methods like electron microscopy and the individual molecules can be revealed. His work has contributed significantly to stopping the progression of HIV into AIDS and to developing new drugs for cancer treatment in both his academic career and through a spinoff company he initially founded with two former students. “In Europe, Australia and the United States, we are lucky, we have access to medicines that research has developed, but the real challenge is to make sure that it’s available not just to the rich, but to the world in general.” Episode recorded: September 26, 2019. Interviewer: Dr Andi Horvath. Producer, audio engineer and editor: Chris Hatzis. Co-production: Silvi Vann-Wall and Dr Andi Horvath. Image: Getty Images.

E10 Seeing With Electrons

Materialism

Play Episode Listen Later Oct 7, 2019 48:50

Episode Notes The optical microscope was discovered in the 17th century and ever since has been a staple of scientific discovery. It uncovered germs and let humanity investigate the microscopic world. However, optical microscopes face a limit to their resolution since they rely on visible light. If materials scientists were going to ever probe atoms, grains, domains, and other features smaller than a few hundred nanometers, it would be necessary to fundamentally change the way we see matter. In this episode we talk about electron microsopes. Their history, how they work, and some of the incredible advantages and features they offer to scientists. There are some great papers out there we pulled from to make this episode. Like this one highlighting the key events in the History of Electron Microscopy. https://doi.org/10.1017/S1431927603030113If you have questions or feedback please send us emails at materialism.podcast@gmail.com. Make sure to subscribe to the show on iTunes, Spotify, google play or wherever you find your podcasts. If you like the show and want to help us reach more people, consider leaving a review - it helps us improve and it exposes new people to the show. Finally, check out our Instagram page @materialism.podcast and connect with us to let us know what new material you’d like to hear about next. We’d like to give a shoutout to AlphaBot for allowing us to use his music within the podcast. Check him out on Spotify. And as always a special thanks to Kolobyte who created the intro and outro for our podcast. He makes a ton of really cool synthwave music which you can check out at kolobyte.bandcamp.com. Support Materialism by donating to their Tip Jar: https://tips.pinecast.com/jar/materialismFind out more at https://materialism.pinecast.co

spotify history electrons electron microscopy alphabot

Back flip, True Lies & DRM

Nerds Amalgamated

Play Episode Listen Later May 27, 2019 55:30

Here we go with another episode from Nerds Amalgamated. This week is packed full of fun stuff to look forward to. First up is it a robot or is it a dog? It is Boston Dynamics student competitors with Standford Doggo. This fabulous little robot is awesome and does tricks, listen in to find out what they are. Also did you want your very own robot doggo? Well you are in luck as we tell you about how to get one. Also check our website for the show notes with hyperlinks, you need the article to get what you need. Next up we have DJ telling us about a proposed new series coming out based on a movie. Yep, another movie is being adapted for your viewing pleasure. It will once again not have the same actors in it that were the main stars in the movie, like so many others out there. But hopefully it will be enjoyable all the same. We won’t hold our breathe but surely they will have learned something over there by now… Who the heck are we kidding, those idiots never listen to anyone else, let alone the proposed viewing public. Next up we look at the blooper that is worthy of a standing ovation. Someone involved with the release of a game from Bethesda studios forgot the DRM. We know, how unlike Bethesda to stuff up something right? BWAHAHA!!! This amazing bit of luck is available on Steam and quite probably numerous other websites that deal in nefarious shadowy dealings. We personally are unaware of the names of such sites and therefore are unable to confirm or deny such suggestions. But come on, just think about it, a brand new game released without the DRM and no one is going to chase that down the rabbit hole of pirating it? Yeah, like Game of Thrones was never pirated ever. We have the usual shout outs, remembrances, birthdays and events from history. Plus games we are playing at the moment. All combined into one big mess that we call the show. We hope you enjoy and as always, stay safe, look after each other and stay hydrated.EPISODE NOTES: Back flipping robot - https://www.futurity.org/doggo-robot-2067152/True Lies TV series reboot - https://www.empireonline.com/movies/news/true-lies-tv-adaptation-heading-disney/DRM - https://steamcommunity.com/games/548570/announcements/detail/2565275416672419265Games currently playingBuck – The Crew 2 - https://store.steampowered.com/app/646910/The_Crew_2/Professor – Cataclysm: Dark Days Ahead - https://cataclysmdda.org/DJ – Steep - https://store.steampowered.com/app/460920/Steep/(edited)Other topics discussedHold my Beer Comedy- http://westender.com.au/circus-coming-hold-beer-end-westend/Flipsy the dog (Simpsons reference)- https://www.youtube.com/watch?v=L_nGJvqHcV8LEGO Mindstorms- https://www.lego.com/en-us/mindstormsHulu might take Marvel shows such as Daredevil - https://www.cinemablend.com/television/2466812/hulu-is-down-to-revive-daredevil-and-other-cancelled-marvel-tv-showsDenuvo - https://en.wikipedia.org/wiki/Denuvo- https://www.howtogeek.com/400126/what-is-denuvo-and-why-do-gamers-hate-it/Red Bull Air Championships- https://en.wikipedia.org/wiki/Red_Bull_Air_Race_World_Championship6ix9ine (rapper)- https://en.wikipedia.org/wiki/6ix9ineCutscene saga (That’s Not Canon Production Podcast)- https://thatsnotcanon.com/cutscenesagapodcastShoutouts20 May 1736 - Westminster Bridge Defies a King and the Church - The Archbishop of Canterbury – head of the Church in England – probably prayed there would never be a bridge across the River Thames at Westminster. But he was not alone. Up to the end of the 17th Century most traffic moved up and down on the river rather than by road. River transport was big business and the men who plied their trade on boats and ferries had a lot to lose from the construction of new bridges. They were backed by the Corporation of London which did not want trade moving to the fringes of London, but claimed its main objections were the loss of custom to the watermen and to the City markets and the danger of the navigation of the river being impeded. One of the claims was that if the watermen lost their jobs there would be fewer readily available seamen for the navy if England went to war. The arguments raged on until in 1664 a major proposal for a bridge was made to the King's Privy Council and to the Lord Mayor. City businesses then played their ace card and bribed King Charles II to scrap the proposal. Officially, it was an interest-free loan, but however the transaction was described the effect was that the building of Westminster Bridge would not take place for nearly 100 years. However, over time various people continued to press for such a bridge until in 1721 petitions went to Parliament. There was the same opposition as before but in the end the case was won and permission to build the bridge finally received Royal Approval on 20 May 1736, when George II was on the throne. Work began in 1738 and the bridge was opened on 18 November 1750. - https://www.onthisday.com/articles/westminster-bridge-defies-a-king-and-the-church21 May 1792 - Mount Unzen on Japan's Shimabara Peninsula, erupts creating a tsunami, killing about 15,000; Japan's deadliest volcanic eruption. - https://en.wikipedia.org/wiki/1792_Unzen_earthquake_and_tsunami21 May 1980 - "Star Wars Episode V - Empire Strikes Back", produced by George Lucas opens in cinemas in UK and North America -https://www.onthisday.com/people/george-lucas 21 May 2004 - Stanislav Petrov awarded World Citizen Award for averting a potential nuclear war in 1983 after correctly guessing Russian early warning system at fault - https://en.wikipedia.org/wiki/Stanislav_Petrov22 May 2019 - Illawarra scientist and inventor Macinley Butson has been featured by the world's biggest video sharing website YouTube for her SMART Armour copper cancer shield fabric. Macinley Butson's SMART (Scale Maille Armour for Radiation Therapy) invention is a device that shields the contralateral breast (the breast not being treated) from excess radiation. As well as being made from high density copper, the shields are handmade. - https://www.abc.net.au/news/2019-05-22/youtube-profiles-teenage-scientist-macinley-butson/11134004Remembrances20 May 2019 – Nikki Lauda, Austrian Formula One driver, a three-time F1 World Drivers' Champion, winning in 1975, 1977 and 1984, and an aviation entrepreneur. He was the only driver in F1 history to have been champion for both Ferrari and McLaren, the sport's two most successful constructors. He is widely considered one of the greatest F1 drivers of all time. As an aviation entrepreneur, he founded and ran three airlines: Lauda Air, Niki, and Lauda. He was a Bombardier Business Aircraft brand ambassador. He was also a consultant for Scuderia Ferrari and team manager of the Jaguar Formula One racing team for two years. Afterwards, he worked as a pundit for German TV during Grand Prix weekends and acted as non-executive chairman of Mercedes-AMG Petronas Motorsport, of which Lauda owned 10%. Having emerged as Formula One's star driver amid a 1975 title win and leading the 1976 championship battle, Lauda was seriously injured in a crash at the 1976 German Grand Prix at the Nürburgring during which his Ferrari 312T2 burst into flames, and he came close to death after inhaling hot toxic fumes and suffering severe burns. However, he survived and recovered sufficiently to race again just six weeks later at the Italian Grand Prix. Although he narrowly lost the title to James Hunt that year, he won his second Ferrari crown the year after during his final season at the team. After a couple of years at Brabham and two years' hiatus, Lauda returned and raced four seasons for McLaren between 1982 and 1985 – during which he won the 1984 title by 0.5 points over his teammate Alain Prost. He died of natural causes at 70 in Zurich. - https://en.wikipedia.org/wiki/Niki_Lauda21 May 1935 - Jane Addams, known as the mother of social work, a pioneer American settlement activist/reformer, social worker, public philosopher, sociologist, public administrator, protester, author, and leader in women's suffrage and world peace. She co-founded Chicago's Hull House, one of America's most famous settlement houses. In 1920, she was a co-founder for the ACLU. In 1931, she became the first American woman to be awarded the Nobel Peace Prize and is recognized as the founder of the social work profession in the United States. She is increasingly being recognized as a member of the American pragmatist school of philosophy and is known by many as the first woman "public philosopher in the history of the United States". In the Progressive Era, when presidents such as Theodore Roosevelt and Woodrow Wilson identified themselves as reformers and social activists, Addams was one of the most prominent reformers. She helped America address and focus on issues that were of concern to mothers, such as the needs of children, local public health, and world peace. In her essay "Utilization of Women in City Government," Addams noted the connection between the workings of government and the household, stating that many departments of government, such as sanitation and the schooling of children, could be traced back to traditional women's roles in the private sphere. Thus, these were matters of which women would have more knowledge than men, so women needed the vote to best voice their opinions. She said that if women were to be responsible for cleaning up their communities and making them better places to live, they needed to be able to vote to do so effectively. Addams became a role model for middle-class women who volunteered to uplift their communities. She died of cancer at 74 in Chicago, Illinois. - https://en.wikipedia.org/wiki/Jane_Addams - https://www.nobelprize.org/prizes/peace/1931/addams/biographical/23 May 1701 - William Kidd, Scottish sailor who was tried and executed for piracy after returning from a voyage to the Indian Ocean. Some modern historians, for example Sir Cornelius Neale Dalton, deem his piratical reputation unjust. He was hanged for his crimes at 47 in Execution Dock,Wapping, London. - https://en.wikipedia.org/wiki/William_KiddFamous birthdays21 May 1948 – Leo Sayer, English-Australian singer-songwriter musician and entertainer whose singing career has spanned four decades. He is now an Australian citizen and resident. Sayer launched his career in the United Kingdom in the early 1970s, and became a top singles and album act on both sides of the Atlantic in the 1970s. His first seven hit singles in the United Kingdom all reached the Top 10 – a feat first registered by his first manager, Adam Faith. His songs have been sung by other notable artists, including Cliff Richard ("Dreaming"). He was born in Shoreham-by-Sea, Sussex - https://en.wikipedia.org/wiki/Leo_Sayer21 May 1960 - Jeffrey Dahmer, also known as the Milwaukee Cannibal or the Milwaukee Monster, was an American serial killer and sex offender. Although he was diagnosed with borderline personality disorder,schizotypal personality disorder, and a psychotic disorder, Dahmer was found to be legally sane at his trial. He was convicted of 15 of the 16 murders he had committed in Wisconsin, and was sentenced to 15 terms of life imprisonment on February 15, 1992. He was later sentenced to a 16th term of life imprisonment for an additional homicide committed in Ohio in 1978. On November 28, 1994, Dahmer was beaten to death by Christopher Scarver, a fellow inmate at the Columbia Correctional Institution. He was born in Milwaukee, Wisconsin. - https://en.wikipedia.org/wiki/Jeffrey_Dahmer22 May 1905 - Bodo von Borries, Germanphysicist. He was the co-inventor of the electron microscope. After World War II , he founded the "Rhine-Westphalia Institute for Electron Microscopy" in Düsseldorf in 1948. In 1949, he was involved in the foundation of the German Society for Electron Microscopy. He was born in Herford,North Rhine-Westphalia - https://en.wikipedia.org/wiki/Bodo_von_BorriesEvents of Interest21 May 1881 - American Red Cross founded by Clara Barton, an organization established to provide humanitarian aid to victims of wars and natural disasters in congruence with the International Red Cross. - https://www.history.com/this-day-in-history/american-red-cross-founded21 May 1927 - Aviator Charles Lindbergh, in the Spirit of St Louis, lands in Paris after the first solo air crossing of Atlantic. - https://www.history.com/this-day-in-history/lindbergh-lands-in-paris21 May 1932 - After flying for 17 hours from Newfoundland, Amelia Earhart lands near Londonderry, Northern Ireland, becoming the 1st transatlantic solo flight by a woman - https://www.history.com/this-day-in-history/earhart-completes-transatlantic-flight22 May 1906 – The Wright brothers are granted U.S. patent number 821,393 for their "Flying-Machine". - Patent - http://invention.psychology.msstate.edu/i/Wrights/WrightUSPatent/WrightPatent.html - https://patents.google.com/patent/US821393A/en- Patent War - https://en.wikipedia.org/wiki/Wright_brothers_patent_war IntroArtist – Goblins from MarsSong Title – Super Mario - Overworld Theme (GFM Trap Remix)Song Link - https://www.youtube.com/watch?v=-GNMe6kF0j0&index=4&list=PLHmTsVREU3Ar1AJWkimkl6Pux3R5PB-QJFollow us on Facebook - https://www.facebook.com/NerdsAmalgamated/Email - Nerds.Amalgamated@gmail.comTwitter - https://twitter.com/NAmalgamatedSpotify - https://open.spotify.com/show/6Nux69rftdBeeEXwD8GXrSiTunes - https://itunes.apple.com/au/podcast/top-shelf-nerds/id1347661094RSS - http://www.thatsnotcanonproductions.com/topshelfnerdspodcast?format=rss

united states america women american game church chicago uk spirit work england japan dj marvel ohio australian russian united kingdom north america illinois wisconsin world war ii game of thrones sea atlantic scottish thrones milwaukee wright steam ferrari flip simpsons corporations parliament f1 bethesda northern ireland daredevil st louis george lucas grand prix westminster zurich sussex aclu theodore roosevelt patent newfoundland nobel peace prize mclaren canterbury jeffrey dahmer dahmer formula one indian ocean american red cross amelia earhart steep true lies woodrow wilson drm boston dynamics utilization addams bodo lord mayor river thames progressive era sayer radiation therapy italian grand prix londonderry james hunt lauda city government leo sayer illawarra alain prost king charles ii scuderia ferrari jane addams flying machines clara barton german tv denuvo international red cross milwaukee cannibal stanislav petrov brabham herford westminster bridge german grand prix shoreham borries amalgamated wapping german society george ii electron microscopy nikki lauda unzen

Cryo-Electron Microscopy and the Complexity of Cancer

HerbRally | Herbalism | Plant Medicine | Botany | Wildcrafting

Play Episode Listen Later Mar 28, 2019 58:24

In this webinar, you will learn: - How structural biology can change the face of your research - The biological challenges of cancer research - Advantages of cryo-EM for your research Covering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure. In single-particle cryo-EM, hundreds of thousands of images formed by electron scattering of individual molecules or complexes are analyzed to derive their three-dimensional structure. Technological and computational advances have dramatically transformed the field of cryo-EM in the past years, enabling structural insights at near-atomic resolution into assemblies that had not been tractable using any other structural biology technique. Consequently, cryo-EM has become a mainstream method structural biology, with a multitude of new facilities and research groups being established all over the world within just a couple of years. This webinar will address the roles that structural biology has been playing in cancer research, uncovering cellular processes involved in cancer development and protection, and guiding drug discovery efforts. The biological challenges of cancer research will be discussed, as well as the unique strengths of cryo-EM as an experimental approach towards these questions, briefly covering the methodology and procedures in sample preparation and data processing. We will illustrate these aspects with some of the latest research from the laboratory of Eva Nogales at UC Berkeley and assess the promises and challenges of cryo-EM in our fight against cancer.

cancer complexity uc berkeley advantages technological cryo electron microscopy eva nogales

49 | An Evening with Ryan Drum: Transgenerational Herbalism and Epigenomics

Play Episode Listen Later Mar 4, 2018 86:43

This recording is from the NorthWest Herb Symposium. Jay and Bridget Johnson of Tree Farm Communications host this excellent event each August in Coupeville, WA. Their next event is August 23rd - 26th, 2018. LEARN MORE CLASS DESCRIPTION Epigenomics is the study of epigenetic changes in gene expression that occur without commensurate changes in DNA base pair sequences; these changes may be transmissible to subsequent generations. Recent research in the emerging field of Epigenomics strongly supports the probabilities of therapeutic transgenerational herbalism. Epigenomics is developing in response to research showing very convincingly that many cancers are initiated and controlled by transmissible extrachromosomal mechanisms. Ryan Drum, PhD has a BSc in Chemical Technology and a PhD in Botany (Phycology) from Iowa State University. While a NATO Scholar, he did postdoctoral studies on Cell Biology using the Electron Microscope and Microcine at the Universities of Bonn, Germany and Leeds, England. For 10 years he taught Botany and related subjects at Universities (UMASS/Amherst, UCLA, WWU). He studied Herbal Medicine with Ella Birzneck, founder of Dominion Herbal College in British Columbia for 12 years, and taught at their summer seminars for 35 years. He has been an adjunct faculty at Bastyr University since 1984, and he lectures at major herbal conferences and herbal schools. Dr. Drum is the author of over 30 scientific papers in peer-reviewed journals, the author of Electron Microscopy of Diatom Cells 1966, Springer Verlag, a 100-Plate Atlas, in print for 20 years, and a contributing author of two chapters in Planting the Future (Gladstar and Hirsch 2001) and a chapter in Fundamentals of Naturopathic Endocrinology, M. Friedman 2005. The proud father of three wonderful children and two fantastic grandchildren, he lives in a rustic little hilltop cabin he built over 40 years ago on a remote island, off the grid, without indoor plumbing or refrigeration. Ryan believes in true patient autonomy: the freedom and right to choose one's caregivers independent of their official certification. VISIT RYAN ONLINE Thanks for listening to the HerbRally Podcast! If you've enjoyed please like, share, subscribe or leave a comment! Thanks and much love. HerbRally www.herbrally.com

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R. Polishchuck - Cutting-edge technology: CLEM: Bridging the light and electron microscopy

FluoMicro 2017

Play Episode Listen Later Jan 12, 2018 54:47

Roman Polishchuck, TIGEM, Naples, Italy speaks on "Cutting-edge technology: CLEM: Bridging the light and electron microscopy". This movie has been recorded at ICGEB Trieste.

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P-32 Correlative Light And Electron Microscopy For Brain Imaging

HistoTalks: NSH Podcasts

Play Episode Listen Later Nov 22, 2017 10:46

This podcast is part of the 2017 NSH Symposium/Convention Poster Podcast Series. The lead author of this poster is Debbie Guerrero. For more information on the author and to view the abstract, visit The Block.

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The 2017 Nobel Prize in Chemistry: Cryo-electron microscopy explained

Reactions

Play Episode Listen Later Oct 4, 2017 1:59

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Ask Herbal Health Expert Susun Weed & Island Herbs Ryan Drum

Ask Herbal Health Expert Susun Weed

Play Episode Listen Later Aug 8, 2017 121:00

Susun Weed answers 90 minutes of herbal health questions followed by a 30 minute interview with Ryan Drum, PhD has a BSc in Chemical Technology and a PhD in Botany (Phycology) from Iowa State University. He studied Herbal Medicine with Ella Birzneck, founder of Dominion Herbal College in British Columbia for 12 years, and taught at their summer seminars for 35 years. He has been an adjunct faculty at Bastyr University since 1984, and he lectures at major herbal conferences and herbal schools. Dr. Drum is the author of over 30 scientific papers in peer-reviewed journals, the author of Electron Microscopy of Diatom Cells 1966, Springer Verlag, a 100-Plate Atlas, in print for 20 years, and a contributing author of two chapters in Planting the Future (Gladstar and Hirsch 2001) and a chapter in Fundamentals of Naturopathic Endocrinology, M. Friedman 2005. this episode Q&A includes: • mints! shiso reseeds itself beautifully, stevia is a mint and prunella vulgaris too.. • tannins tan.. • catnip tincture makes a wonderful bug repellant.. • comfrey strengthens skin, bones, ligament, mucous membranes of respiratory and digestive systems.. • soy milk used as a medicine not a food prevents damage from radiation.. • mentored students- check out herbs for cancer treatment- information can also be found on Time Monk Radio airing this month.. • ssri's- placebos with bad side effects? hypericum out performs out performs them all when taken in tincture form from fresh flowering plant.. • very elevated blood pressure- passionflower, diuretics, lack of sleep.. • restoring eyesight and preventing eyesight loss with hawthorn, amla, carotenes.. • burdock tincture should have a white film containing inulin :)

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Mouse Whole-Brain Volume Electron Microscopy for Cellular Co