Podcasts about second harmonic generation

A peacock in the hand is worth two in the verdant brush... new sounds from Tapes And Topographies and Second Harmonic Generation.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.07.284703v1?rss=1 Authors: Hanrahan, N., Lane, S. I. R., JOHNSON, P. B., Bourdakos, K. N., Brereton, C. J., Ridley, R. A., Davies, E. R., Hosny, N. A., Spickermann, G., Forster, R., Malcolm, G., Davies, D. E., Jones, M. G., Mahajan, S. Abstract: Light sheet microscopy (LSM) has emerged as one of most profound three dimensional (3D) imaging tools in the life sciences over the last decade. However, LSM is currently performed with fluorescence detection on one- or multi-photon excitation. Label-free LSM imaging approaches have been rather limited. Second Harmonic Generation (SHG) imaging is a label-free technique that has enabled detailed investigation of collagenous structures, including its distribution and remodelling in cancers and respiratory tissue, and how these link to disease. SHG is generally regarded as having only forward- and back-scattering components, apparently precluding the orthogonal detection geometry used in Light Sheet Microscopy. In this work we demonstrate SHG imaging on a light sheet microscope (SHG-LSM) using a rotated Airy beam configuration that demonstrates a powerful new approach to direct (without any further processing or deconvolution) 3D imaging of harmonophores such as collagen fibres in biological samples. We provide unambiguous identification of SHG signals on the LSM through its wavelength and polarisation sensitivity. In a multimodal LSM setup we demonstrate that SHG and two-photon signals can be acquired on multiple types of different biological samples. We further show that SHG-LSM is sensitive to changes in collagen synthesis within lung fibroblast 3D cell cultures. This work expands on the existing optical methods available for use with light sheet microscopy, adding a further label-free imaging technique which can be combined with other detection modalities to realise a powerful multi-modal microscope for 3D bioimaging. Copy rights belong to original authors. Visit the link for more info

Rising like water upon a harlequin river. New music on Cryo Chamber, with sounds from Beyond the Ghost, Dronny Darko, Council of Nine, Purl and protoU and Second Harmonic Generation.

Rising like water upon a harlequin river. New music on Cryo Chamber, with sounds from Beyond the Ghost, Dronny Darko, Council of Nine, Purl and protoU and Second Harmonic Generation.

  The 380th of a series of weekly radio programmes created by :zoviet*france:   First broadcast 19 October 2019 by Resonance 104.4 FM, Resonance Extra and CJMP 90.1 FM   Thanks to the artists included here for their fine work.   track list … :zoviet*france: - A Duck in a Tree Link 380a 00 Zach Zinn - Intro 01 Beepblip - Interactions 02 Joaquín Gutiérrez Hadid - Corps 03 Antoine Bertin - At the Harper Asprey Wildlife Rescue 04 Robert Barry - Portraits (extrait), 1995, 3'13" 05 Mystified - Cacoon Stasis 06 Salford Media City vs Noise Research - Doing Stuff Is Fun 07 Fluorescent Grey - Ocampa 08 The Sunken Hum - The Sunken Hum Broadcast 15 – At the Goat's Grave, Collecting Up her Bones – 15 January 2013 09 Second Harmonic Generation ft Tribes of Europe - We Rise Like the Water 10 Walter Tilgner - Waldkauz-Balz 11 Luc Ferrari - Und so weiter – IV 12 Martin Rach - 2+Pi 13 Zach Zinn - That Rose Colored Space ++ Zach Zinn - Intro … :zoviet*france: - A Duck in a Tree Link 380b    

An efflorescence of biologies, with Second Harmonic Generation, Mystified and Aes Dana... and showcasing "The Biode" - new from Robert Rich.

An efflorescence of biologies, with Second Harmonic Generation, Mystified and Aes Dana... and showcasing "The Biode" - new from Robert Rich.

We look at the phase matching condition for Second Harmonic Generation and also do a tuning curve example for an OPO