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Show notes: thetaxprofessionalspodcast.com/TTPP56Subscribe: thetaxprofessionalspodcast.com/subscribeTake control of your career progression free course: https://documents.thetaxprofessionalspodcast.com/freecourseYou'll LearnAn overview of Chris's career and why he decided to set up his own practice(10:15) How Chris went about getting his first clients (and what he learnt)(19:00) How to support and help trainees develop, without compromising the work that you need to do(26:45) Why he decided to transfer ownership of Maslins to an employee ownership trust(41:00) How Chris has created other tax businesses (the word tax being used loosely here!)(54:15) Career advice he'd give his younger self and advice to people towards the start of their careersResourcesAll Tax Career Success Secrets episodes: thetaxprofessionalspodcast.com/TCSS
In this episode we interview three of our Instructional Coaches who presented at NCSS & TCSS in Austin this past November. They discuss their experiences developing & presenting their session as well as reflections on attending the conference. They leave us with sound advice and encourage other educators to share their learning to a larger audience.
We got a special joint for all of y'all as we sit down with surfboard shaper Ryan Lovelace () as he tells us about his beginnings in shaping, what he's learned along the way and how important hand done craft should be. We also sit down with new @WSL longboard tour director, our homie, Devon Howard as he drops the 411 on what's going on with the tour. Lastly we have a couple of short takes with @TCSS, @grantnoble_, @robotsfrom, @beachbox_surf, and what's going on with an initiative to drill for oil in the Australian Blight. This episode is brought to you by our homies over at Nicaragua Craft Beer Make sure to check us out at https://www.instagram.com/bodegaboardercrew/ Collection available here http://www.bodegaboardercrewstore.com Sticker packs via bodegaboardergrew@gmail.com No need to bust a craze on some waves...there's always more during the next swell. TRACKS PLAYED THIS EPISODE 1. Wylin Out (Instrumental) - Urban Renewal Program 2. Police State - Dead Prez 3. Where I'm From - Main Flow & 7L 4. Self Taught - Brother Ali 5. Quality Control - Jurassic 5 6. One for the Fam - Floy Da Locsmif 7. The Feels - Marlon Craft 8. The Weekend - Unknown Prophets 9. Stahfallah - Smif n Wessun 10. Nowhere Fast - Blackalicious 11. Love's Ghetto - Ayatollah 12. Ghetto Rock - Mos Def 13. The Perfect Beat (feat. KRS-ONE) - Talib Kweli 14. Fre$h (feat. Red Alert, PMD, Grand Puba, KRS 1 and Big Daddy Kane) - Cormega 15. Harbor Masters (feat. AZ) - Wu-Tang 16. Gobstopper - J Dilla 17. Unspoken - Literates 18. Steppin' It Up (feat. Busta Rhymes & Redman) - A Tribe Called Quest Til next time suckas... keep those toes wet #JustTheTip #ToesBeforeHoes Merch available here http://www.bodegaboardercrewstore.com and follow us on Instagram https://www.instagram.com/bodegaboardercrew/ RYAN LOVELACE LINKS: https://www.youtube.com/watch?v=wOtTdp0axrY https://www.youtube.com/watch?v=16_1edoxlFE https://vimeo.com/255828417 SHORT TAKES: TCSS clip with Ryan Glover https://www.thecriticalslidesociety.com/blogs/news/promised-land-ryan-glover-jarrod-saville Robots From clip of Grant Noble for RVCA https://vimeo.com/315768365 Beach Box storage shower Kickstarter http://www.bbse.fnd.to/beachbox Keep Equinor out of the Australian Bight https://www.nopsema.gov.au/environmental-management/open-for-comment-stromlo-1-exploration-drilling-program-draft-environment-plan/
Coming to you live and direct from #TheNorthShore is an extended version of the podcast. On this episode we sit down with Devon Howard (@devon_howard) and talk about getting into logging, proving yourself on a log, how to keep the stoke alive and much more. We also have some short takes on the Kelly Slater (@kswaveco) wave pool being a @WSL CT event, Sharks popping up on cams and a short clip about #VanLife from Mick Rogers (@mrrodgers_neighborhood) and TCSS (@tcss). Also make sure to stay tuned to the very end for a special surprise freestyle joint we dug up from the personal archives. Make sure to check us out at https://www.instagram.com/bodegaboardercrew/ Merch available here http://www.bodegaboardercrewstore.com Sticker packs via bodegaboardergrew@gmail.com No need to bust a craze on some waves...there's always more during the next swell. TRACKS PLAYED THIS EPISODE 1. Boom Biddy Bye Bye (Fugees Mix Instrumental) - Cypress Hill 2. Ya Slippin' - Boogie Down Productions 3. Pubic Enemy - A Tribe Called Quest 4. Looseys - Das EFX 5. Auditorium (feat. Slick Rick) - Mos Def 6. The Mission - Special Ed 7. Top Billin' (Instrumental) - Audio Two 8. Apartheid (feat. Buckshot & General Steele) - Sean Price 9. Feel Me (feat. Bun B & Rock of Heltah Skeltah) - Pete Rock & Smif-n-Wessun 10. Thuggish Ruggish Bone - Bones Thug n Harmony 11. Coolie High - Camp Lo 12. Put It On - Big L 13. Hands Up - DJ Premier 14. It's My Thing - EPMD 15. 7th Chamber - Wu-Tang Clan 16. Smoke Buddah - Redman 18. Freestyle - Ghostface Killah and Method Man Til next time suckas... keep those toes wet #JustTheTip #ToesBeforeHoes Merch available here http://www.bodegaboardercrewstore.com and follow us on Instagram https://www.instagram.com/bodegaboardercrew/ MAKE SURE TO CHECK OUT Devon Howard https://open.spotify.com/user/128749040/playlist/46qDBObbIwUiZLC05AAuhD https://vimeo.com/184749376 https://vimeo.com/129403517 https://www.instagram.com/p/Ba9VNfcA6Rc/?taken-by=devon_howard https://www.instagram.com/p/BVhav_VgPfP/?taken-by=devon_howard https://www.instagram.com/p/BXd2WbVA3Y7/?taken-by=devon_howard https://www.instagram.com/p/BUeofScAZJ2/?taken-by=devon_howard https://vimeo.com/117554484 https://www.instagram.com/p/BVQ4JnRAnss/?taken-by=devon_howard https://www.instagram.com/p/BOVVwoRDCQ4/?taken-by=devon_howard SHORT TAKES Shark Breach in Venice https://new.surfline.com/surf-news/watch-shark-breach-venice-beach/13839 Wavepool part of the WSL 2018 schedule https://new.surfline.com/surf-news/kelly-slaters-wavepool-officially-world-tour-stop/13764 JoyRide https://vimeo.com/219464556 HELP PUERTO RICO!!! https://secure.savethechildren.org/ http://unidosporpuertorico.com/en/ https://support.crs.org/donate/hurricane-irma-relief?gclid=EAIaIQobChMI14-6kPG21gIVSySGCh0Bmg3yEAAYAiAAEgImSvD_BwE
Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 05/06
In their habitats, microorganisms are often in competition for limited nutrients. In order to succeed, many Gram-positive bacteria resort to production of peptide antibiotics. Therefore, resistance mechanisms against these compounds are essential. The first step of ensuring survival is the perception of the harmful drugs and mediation of resistance against it. In recent years, a group of ABC-transporters have been recognized as important resistance determinate against antimicrobial peptides. The expression of these transporters is generally regulated by a two-component system, which in most cases is encoded next to the transporter. Together they are described as detoxification modules. The permeases of the transporters are characterized by a large extracellular domain, while the histidine kinases lack an obvious input domain. One of the best understood examples is the BceRS-BceAB system of Bacillus subtilis, which mediates resistance against bacitracin, mersacidin and actagardine. For this system it was shown that the histidine kinase is not able to detect the substrate directly and instead has an absolute requirement for the transporter in stimulus perception. This describes a novel mode of signal transduction in which the transporter is the actual sensor and therefore regulates its own expression. To date, mechanistic details for this unique mode of signal transduction remain unknown. Several other examples have been described for transport proteins that have acquired additional sensing or regulatory functions beyond solute transport, and these have been designated trigger transporters. For these bifunctional transporters a direct protein-protein interaction with membrane-integrated or soluble components of signal transduction relays has been postulated. However, for most sensor/co-sensor pairs, conclusive proof of such an interaction is lacking, and so far little is known about the sites that might mediate contacts between the putative protein interfaces and how communication is achieved. Based on sequence and architectural similarities, we identified over 250 BceAB-like transporters in the protein database, which occurred almost exclusively in Firmicutes bacteria. To whether the regulatory interplay between the ABC transporter and the two-component system was a common theme in these antimicrobial peptide resistance modules, we carried out a phylogenetic study of these identified systems. We identified a clear coevolutionary relationship between transport permeases and histidine kinases. Furthermore, we identified conserved putative response regulator binding sites in the promoter regions of the transporter operons. Additionally, we were able to provide a tool to identify TCSs for transporters lacking a regulatory system in their genomic neighbourhood, which was based on the coclustering of histidine kinases and transporter permeases. These findings also suggested the existence of a sensory complex between BceAB-like transporters and BceS-like histidine kinases. To further investigate the signaling mechanism, we performed a random mutagenesis of the transport permease BceB with the aim to identify regions or residues within the transporter that are involved in signaling and/or resistance. With this approach we were able to identify mutations that affected either the ability for signaling or mediation of resistance. This showed a partial genetic separation of the two qualities, which could be achieved by single amino acid replacements. These results provide first insights into the signaling mechanism of the Bce system. In order to analyse the proposed communication between two-component system and ABC transporter, we further characterized their interactions by in vivo and in vitro approaches. We could demonstrate that the transporter BceAB is indeed able to interact directly with the histidine kinase. Because it was unknown how the signal perception by BceAB-type transporters occurs, we next analyzed substrate binding by the transporter permease BceB and could show direct binding of bacitracin by BceB. Finally, in vitro signal transduction assays indicated that complex formation with the transporter influenced the activity of the histidine kinase. In summary this thesis clearly shows the existence of a sensory complex comprised of BceRS-like two-component systems and BceAB-like ABC transporters and provides first functional insights into the mechanism of stimulus perception, signal transduction and antimicrobial resistance mechanism employed by these wide spread detoxification systems against antimicrobial peptides.
Onsite at the Tuscaloosa City Board of Education, covering TCT Site Selection. This is expected to be an intense meeting as the Board of Education votes on the site for the new Center for Technology. The Old Westlawn Middle School has emerged to be the best location to meet the 10 acre requirement and at a lesser cost. The location in West Tuscaloosa is apparently a problem for the white students who currently make up 22% of the TC student population. 73% of the students currently attending TCS are black, and 3% other. Adding to the smoldering fire are reports of secret meetings to find another site; and rumors about TCSS employees making racially charge statements against the community where Old Westlawn Middle School is located; and emails and phone calls asking white parents and students to voice their objections to locating TCT in West Tuscaloosa. The surface argument is about distance and travel time. The white students favor the Tuscaloosa Magnet School Site over Old WMS. The miles difference in the sites for all schools are less than 2 miles. Time difference is 3 minutes for Northridge and Bryant, 1 minutes for Central students.