Biochemistry Class Notebook

Better know this stuff!

I welcome your comments here after or before your class. Suggestions for topics and clarification may be and are encouraged to be posted here. You can be anonymous or you may identify yourself. I especially would like to see comment strings, that is, what you think about what someone else has said. Come here often to review material covered in lecture.

Much of chapter 1 is a review of material you should have seen before with respect to thermodynamics and Gibbs Free Energy.

Chapter 1 contains a discussion of thermodynamics. Your comments are always welcomed. Enthalpy, entropy and free energy changes lie underneath all biochemical reactions driving them to completion and initiating the next reaction. Carefully read your chapter and search for other sources to reinforce these concepts.

So here's a little description of what I believe explains the difference between&Delta G and &Delta G0.Gibb's Free Energy

Here is a quick review of pH, pK and pI that will be important when we begin Chapter 4: amino acids.Amino Acid Titration.For Window Media Players

Here is a summary of some important details concerning amino acid structure and properties. You should also be able to download theses videos for free from the iTunes music store if you have an account. You will find it in the Podcast section under "Biochemistry Class Notebook". Stevie...what more can I say.

After discussing amino acids, protein primary, secondary and tertiary structure, we will discuss protein structure and function using hemoglobin: the classical allosteric protein.

Here's a brief overview of the most challenging topic in chapter 7, The Bohr Effect.

Here is the first of 2 installments on our Enzyme Kinetics lectures. Use this for a quick review of the terms KM, VMAX, Kcat, KS and enzyme efficiency. More description of the Linweaver-Burke and inhibition will follow in the 2nd installment.

Hope you listen in on this description of the 3 common enzyme inhibition profiles, it may prove helpful on Exam III.

(click the title to play or right click it to download and play in Quicktime)The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part I before coming to class.If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there.

(click the title to play or right click to download and play in Quicktime)The first experiment in Biochemistry lab this coming week is the Spectrophotometry of Riboflavin. Take a look at the Spectrophotometry of Riboflavin, Part II video before class.If you're having trouble downloading and viewing the link on the title then this link will redirect you to the source file and you should be able to hear and see it there.

Read your manual and do your calculations before class. Let me know if you have trouble viewing the Quicktime movie.

The amino acid titration experiment is described in this 20 minute video. CLICK the title to download and play a windows media file (not very good). Here you can download and play the quicktime file or get it from iTunes. Finally to accommodate all of you you may be able to view this video below. Go through it before taking the bonus quiz.

In the Experiment IV video will take a stock solution of BSA to generate a standard curve to use to determine the concentration of an unknown concentration of BSA. BSA is a standard size protein and is used regularly as a control to compare and determine concentrations of many different types of pure proteins. Can you tell me what BSA stands for?

Experiment VI and part of Experiment VII are described in this video. There is forthcoming the actual loading, running, staining and destaining of the gel before the final so stay tuned. You can access this video from iTunes or click the title here and view it or download it from my archives if having trouble viewing here. It may be necessary to wait until the video completes buffering to get a smooth view.