Podcasts about electrophoresis

The start of Season 3 is just around the corner, slated to start in January 2025. What's the new season hold in store you might ask? Steve assures us he'll be back hosting Season 3 and teases at topics that are coming. These include biosecurity, bioengineering, polymer degradation, genetics of obesity, CROs and how they utilize mol bio, platelet biology, and epitranscriptomics. As if that were not enough to get you excited about the new season, Steve also teases on the inclusion of Mol Bio Minutes mini episodes in the upcoming season. These short but oh, so helpful episodes will cover molecular biology lab techniques – sure to be a hit with those working in the lab. Subscribe to get future episodes as they drop and if you like what you're hearing we hope you'll share a review or recommend the series to a colleague.  Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology. For Research Use Only. Not for use in diagnostic procedures.

This week's episode will be focusing on Hemoglobin (Hb) electrophoresis. We will go important details on what Hb electrophoresis is, the normal breakdown of adult hemoglobin, and specific hemoglobinopathies (thalassemias, sickle cell, Lepore...) you can be tested on during your ITEs and hematology boards.

Opinionated Science welcomes Paulius Palaima for a remarkable behind-the-scenes look into the world of Electrophoresis. Lucy and Paulius discuss the Invitrogen E-gel Power Snap Plus electrophoresis system, and the features and benefits in both high- and low-throughput applications...there's even a mention of Pokémon to listen out for! Listen to the full episode now.     

Edición Limitada - 20 de Junio del 2022. Producción, realización y conducción: Francisco J. Brenes. Presentando música de Sun's Signature, JB Dunckel, Zola Jesus, Jack White, A.A. Williams, And You Will Know Us By the Trail of Dead, Bastille, Sinead O'Brien, Gaz Coombes, Dream Dali, Editors, Belle and Sebastian, Lightning Seeds, Simple Minds, Buzzcocks, Dummy, Dry Cleaning, Tim Burgess, Pixies, The Beths, Dion Lunadon, High Vis, Metric, Young Guv, Bartees Strange, Kula Shaker, Declan Welsh And The Decadent West, Melts, William Orbit con Polly Scattergood, Chemical Brothers, Rosie Thorne, Flasher, Röyksopp con Karen Harding, Hercules & Love Affair, Utah Saints, Neu!, Electrophoresis con Molchat Doma, Front Line Assembly, Adult. y Black Magnet.

In this second episode of HardwareX Season 2 podcasts, our guest is Diego Lagos-Susaeta who is affiliated with the Centre for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering, Biotechnology and Materials, University of Chile, Santiago, Chile. Our guest is the first author of an HardwareX article titled “openPFGE: An open source and low cost pulsed-field gel electrophoresis equipment” along with co-authors  Oriana Salazar and Juan A. Asenjo from the same university. For this episode, the host and content creator is Dr. Sanli Faez who is an Assistant Professor of Physics at Utrecht University. The post-editing and production was handled by Dr. Santosh Pandey who is an Associate Professor in Electrical Engineering at Iowa State University. The theme music is provided by Oleksandr Savochka from Pixabay.

Electrophoresis techniques allow for separation and reuse in a number of industrial and biomedical processes. What can they do for drilling fluids?

Electrophoresis techniques allow for separation and reuse in a number of industrial and biomedical processes. What can they do for drilling fluids?

Introduction to Genetic Engineering, chapter 3. Electrophoresis, DNA/RNA isolation, gene sequencing, cellular preparation, biochemical considerations. --- Support this podcast: https://anchor.fm/osuz504-tech/support

This topic is part of chapter-21 of the book"Analytical Chemistry", written by G. D. Christian and others

This topic has been described from the book, "Analytical Chemistry", by G. D Christian and others

My AP Biology Thoughts  Unit 6 Gene Expression and RegulationWelcome to My AP Biology Thoughts podcast, my name is Sid and I am your host for episode #112 called Unit 6 Gene Expression and Regulation: Biotechnology. Today we will be discussing how we use technology to study how the mechanisms of DNA and gene expression work. Segment 1: Introduction to BiotechnologyThe four main processes used in biotechnology that relate to this unit are bacteria transformation, PCR, electrophoresis, and DNA sequencing. Bacterial transformation makes multiple copies of a recombinant DNA molecule. PCR is used to produce millions of copies of a DNA sequence from an initial sample. Electrophoresis separates DNA and RNA molecules by their size and their electrical charge. DNA sequencing is used to determine the sequence of the bases in a DNA molecule. Segment 2: More About BiotechnologyFirst we'll talk about bacterial transformation. The process of bacterial transformation starts with mixing the prepared bacteria with DNA. Then the bacteria are heat shocked. This allows them to take up a plasmid. The bacteria that take up the plasmid become resistant to antibiotics, so we place all of the bacteria on an antibiotic plate. The ones that survive are the ones that are known to have taken up the plasmid since they survived the antibiotic. The bacteria without the plasmid end up dying. The bacteria that survived end up being used to create a cluster of identical bacteria that also contain the plasmid. The colony containing the plasmid is grown and used to produce the plasmid or proteins. Another form of biotechnology is PCR. To begin, the main ingredients (taq polymerase, primers, template DNA, nucleotides, and cofactors) are all added in a tube. The first step of PCR is denaturation. In denaturation the reaction is heated so that the DNA strands separate and create single strands. The next step is annealing where the reaction is cooled so that the primers bind to the complementary sequence on the DNA strands. The third step is extension. In this step the temperature is raised again so that the taq polymerase starts at the primers and synthesizes new strands of DNA. This cycle repeats between 25-35 times which ends up creating millions of copies of the same DNA region. Electrophoresis is another form of important bio technology. In electrophoresis, DNA samples are placed into indentations at one end of a gel. THis gel gets an electric current applied to it. Since DNA fragments are negatively charged, they move towards the positive electrode. Because the DNA fragments have the same charge, the smaller fragments are able to move through the gel faster than the large ones. This allows the DNA to be separated by size. The gel is then stained with a DNA binding dye which makes the DNA fragments appear as bands so that they can be observed. The last thing we are going to talk about is DNA sequencing. In DNA sequencing, the DNA strand goes through bacterial transformation so that we can produce many copies of it in a plasmid. The DNA is then isolated and goes into a plate with other ingredients like the DNA bases, DNA polymerase, primers, and modified bases labeled with colored fluorescent tags called terminator bases. This mixture then goes through a process very similar to PCR. The difference is when polymerase adds the bases, it eventually adds a terminal base which makes it so that no more bases can be added to the strand of DNA. This then produces lots of fragments of DNA at different lengths. Then when we separate them by size through electrophoresis, we can figure out where the beginning of the sequence is. When the fragments are separated in the gel, a laser reads the terminator base of each strand which gives us the full sequence of bases Segment 3: Connection to the Course It's important for us to know how these different processes work because it helps us understand how we apply the things we know about gene expression and...

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.27.270405v1?rss=1 Authors: Qiu, D., Wilson, M. S., Eisenbeis, V. B., Harmel, R., Riemer, E., Haas, T. M., Wittwer, C., Jork, N., Gu, C., Shears, S. B., Schaaf, G., Kammerer, B., Fiedler, D., Saiardi, A., Jessen, H. J. Abstract: The analysis of myo-inositol phosphates (InsPs) and myo-inositol pyrophosphates (PP-InsPs) is a daunting challenge due to the large number of possible isomers, the absence of a chromophore, the high charge density, the low abundance, and the instability of the esters and anhydrides. Given their importance in biology, an analytical approach to follow and understand this complex signaling hub is highly desirable. Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. Stable isotope labeled (SIL) internal standards allow for matrix-independent quantitative assignment. The method is validated in wild-type and knockout mammalian cell lines and in model organisms. SIL-CE-ESI-MS enables for the first time the accurate monitoring of InsPs and PP-InsPs arising from compartmentalized cellular synthesis pathways, by feeding cells with either [13C6]-myo-inositol or [13C6]-D-glucose. In doing so, we uncover that there must be unknown inositol synthesis pathways in mammals, highlighting the unique potential of this method to dissect inositol phosphate metabolism and signalling. Copy rights belong to original authors. Visit the link for more info

GE find us the length of a DNA Strand (how many base pairs long).

Episode 33 recAPs genetic engineering techniques of biotechnology. Electrophoresis separates molecular fragments according to size and charge visually (1:00). PCR amplifies DNA fragments, making thousands of copies from even the smallest sample (2:35). Bacterial transformation introduces foreign DNA into bacterial cells (4:00). DNA sequencing is a part of biotechnology, but typically not working with an entire genome at one time (6:15).The Question of the Day asks (8:08) “Which cells in the human body do NOT contain nuclear DNA?Thank you for listening to The APsolute RecAP: Biology Edition!(AP is a registered trademark of the College Board and is not affiliated with The APsolute RecAP. Copyright 2020 - The APsolute RecAP, LLC. All rights reserved.)Website:www.theapsoluterecap.comEMAIL:TheAPsoluteRecAP@gmail.comFollow Us:INSTAGRAMTWITTER

David Sanders, Associate Professor in the Department of Biological Sciences at Purdue University, works on introducing genes, or nucleic acids (DNA and RNA), into cells with the hope of producing a therapeutic benefit to treat disease. Using the virus as a shell, David can manipulate different properties of a virus. One modification involves changing the proteins on the outside of the virus. The modified virus can then be used as a delivery vehicle for introducing genes into particular targeted cells. Different viruses target different types of cells: some viruses want to go into white blood cells, liver cells, brain cells, or cells in the respiratory tract. Gene therapies are probably best known for their use in treating genetic diseases such as sickle cell anemia and cystic fibrosis. However, gene therapy can also be used to treat cancer and, of current interest, to generate an immune response against coronaviruses. Viruses have perfected the technique of bringing nucleic acids into cells. Instead of having viruses transmit their own nucleic acids, David modifies them to transfer preferred genes. David refers to these as “mix and match viruses” because they take certain traits from different viruses to make an ideal combination for delivering gene therapy. One safety modification used in his lab is to fix the viruses so that they can’t reproduce themselves. David’s group has worked on a virus that acts like a retrovirus on the inside. Retroviruses go backward from RNA to DNA, meaning the gene a retrovirus delivers becomes permanently incorporated into the target cell. The protein on the outside of the virus that David’s group studies was replaced with a protein found on the outside of the Ebola virus. This allowed his group to target cells found in the lung airway epithelium where it would be ideal to target gene delivery in order to cure cystic fibrosis.  Another interest of David’s involves the ethics surrounding the process of scientific publication and the peer review process. David comments on safeguards that can be used to help prevent bias within research science. It’s important for the scientists who first come up with an idea to be properly credited for their work.       Link to worksheet: https://docs.google.com/document/d/1ypanmpf8jsepFQOKdwycT4cncryURg2epUN4_sazwE0/edit?usp=sharing

Protein electrophoresis is commonly ordered for patients with suspected or known plasma cell disorders. Pathologists may sometimes be unsure of the sequence in which tests should be ordered when evaluating for a plasma cell neoplasm, and it’s important to understand the utility and the limitations of each test, and its unique role in arriving at an accurate conclusion. In this CAPcast, Dr. Diana Desai provides an overview of protein electrophoresis and some challenges that pathologists should be aware of when ordering these tests. Dr. Desai developed a course on this topic as part of the CAP’s Clinical Laboratory Improvement Program, or CPIP (https://capatholo.gy/32LNx80).

Serum protein electrophoresis and immunofixation electrophoresis are critical laboratory assays in the identification of monoclonal proteins.  Monoclonal proteins, also called “M spikes” or “paraproteins,” may be due to monoclonal gammopathy of undetermined significance, but they may also indicate malignant diagnoses such as multiple myeloma or lymphoplasmacytic lymphoma. Protein electrophoresis separates the proteins in a patient sample by their size and charge and allows for the detection of abnormal monoclonal proteins.  Immunofixation electrophoresis is used to determine the type of monoclonal protein present.  Both methods are technically challenging and require interpretation by a qualified laboratory professional. Occasionally, anomaly such as immunoglobulin complexes or temperature sensitive cryoglobulins distort the protein separation and prevent accurate interpretation of test results. A case study published in the July 2019 issue of the Journal of Applied Laboratory Medicine describes a patient whose monoclonal protein was not identifiable by routine protein electrophoresis and immunofixation methods.  The Case Report suggests steps that laboratories may take to reduce artifacts and identify monoclonal proteins accurately.

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Professor Lander explains methods of analyzing a gene of interest after it has been cloned.

Professor Lander continues with the discussion of DNA Sequencing technologies, methods of analyzing DNA sequencing data, and the process of Polymerase Chain Reaction (PCR) and its applications.

Vincent, Elio, Michael, and Michele discuss the diel transcriptional rythmns of bacterioplankton communities in the ocean, and extensively drug resistant Pseudomonas in Ohio.

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler Vincent, Alan, Rich and Kathy review a protease essential for influenza pathogenesis in mice, and directionality of rhinovirus RNA exit from the capsid. Links for this episode Protease essential for influenza pathogenesis in mice (PLoS Path) Rhinovirus uncoating is directional (PLoS Path) Fluorescence correlation spectroscopy Capillary electrophoresis (Wiki) Letters read on TWiV 267 Weekly Science Picks Kathy - Amazing mapsAlan - Florida to NJ in 156 seconds (YouTube)Rich - Whiteout over Great Lakes from SpaceVincent - LORiOLA viral necklaces Listener Pick of the Week Carol - Knit picornavirusRobert - RNA: Life's Indispensible Molecule by James Darnell,and Biochemical Pathways by Michal and Schomburg Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Using biology techniques to solve a crime. License: Creative Commons BY-NC-SA More information at http://k12videos.mit.edu/terms-conditions

Hungry for Knowledge: Oliver Smithies is a toolmaker. He shared the Nobel prize for discoveries that led to the development of knockout mice. Diego Bohorquez uses mouse models to understand how our gut regulates appetite. He has wanted to meet Smithies ever since he moved from his native Ecuador to Duke University in the United States. When the two meet in Lindau they have an instant rapport and soon they're sharing ideas about their research projects and talking about what makes a successful scientific collaboration.

Experiment VI and part of Experiment VII are described in this video. There is forthcoming the actual loading, running, staining and destaining of the gel before the final so stay tuned. You can access this video from iTunes or click the title here and view it or download it from my archives if having trouble viewing here. It may be necessary to wait until the video completes buffering to get a smooth view.

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The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of the gels were employed: (1) The DNA mass distribution profile obtained by electrophoresis was compared to computed profiles, and the number of DSB per unit length was then derived in terms of a fitting procedure; (2) hybridization of selected chromosomes was performed, and a comparison of the hybridization signals in treated and untreated samples was then used to derive the frequency of dsb.

Fri, 1 Jan 1988 12:00:00 +0100 http://epub.ub.uni-muenchen.de/3083/ http://epub.ub.uni-muenchen.de/3083/1/040.pdf Ragoussis, Jiannis; Bloemer, Katharina; Weiß, Elisabeth; Ziegler, Andreas Ragoussis, Jiannis; Bloemer, Katharina; Weiß, Elisabeth und Ziegler, Andreas (1988): Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis. In: Immunogene

Sat, 1 Jan 1983 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9316/1/9316.pdf Cremer, Thomas; Cremer, Marion; Grund, Christoph; Moll, R.; Franke, W. W.;

Sat, 1 Jan 1972 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7517/1/7517.pdf Neuhoff, V.; Dames, W.; Cremer, Thomas