Podcasts about cas1

Scott Zastrow was an important part of one of the most important Special Forces Teams that ever was- the Triple Nickel, or ODA 555. The things they did were so legendary that they made a movie about it. As part of the first Special Forces team that responded to the September 11th, 2001 attacks, Scott was part of one of the most amazing stories to ever come out of the Global War on Terror. Thank you to Scott for coming on, make sure to check out his book on Amazon or wherever you get your books!!!00:00 The Intro, the Movie, and the Book The Dequello 01:22 Aaron and Scott were BS'ing and the episode started and they didn't really pay attention 09:10 Move to Fort Benning and getting into SF 15:00 I quit every day, I just didn't tell anyone 19:15 Training 18 deltas 24:30 Getting to 5th Group 42:55 Great Story 49:00 September 11 and getting ready to go1:00:00 Getting to K2 and into Afghanistan 1:26:00 Beating the system with CAS1:53:00 Taking Kabul2:15:00 Everyone is full of crap2:27:00 POVATCollabs:18A Fitness - Promo Code: 1ReadyAlpha Brew Coffee Company - Promo Code: ONESREADYATAC Fitness - Promo Code: ONESREADY10CardoMax - Promo Code: ONESREADYEberlestock - Promo Code: OR10Hoist - Promo Code: ONESREADYStrike Force Energy - Promo Code: ONESREADYTrench Coffee Company - Promo Code: ONESREADYGrey Man Gear - Promo Code: ONESREADY The content provided is for informational purposes only and does not constitute legal advice. The host, guests, and affiliated entities do not guarantee the accuracy or completeness of the information provided. The use of this podcast does not create an attorney-client relationship, and the podcast is not liable for any damages resulting from its use. Any mention of products or individuals does not constitute an endorsement. All content is protected by intellectual property laws. By accessing or using this you agree to these terms and conditions.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.30.361584v1?rss=1 Authors: Long, C., Dai, L., E, C., Da, L.-T., Yu, J. Abstract: Cas1 and Cas2 are highly conserved proteins across CRISPR-Cas systems and play a significant role in protospacer acquisition. Here we study the protospacer (or ps) DNA binding, recognition, and response to cleavage on the protospacer-adjacent-motif complementary sequence or PAMc by Cas1-Cas2, implementing all-atom molecular dynamics simulations. First, we noticed that two active sites of Cas1&1' bind asymmetrically to two identical PAMc in the simulation. For psDNA containing only one PAMc to be recognized, it is then found that the non-PAMc association site remains destabilized until after the bound PAMc being cleaved. Thus, correlation appears to exist between the two active sites, which can be allosterically mediated by psDNA and Cas2&2' in bridging. To substantiate such findings, we further simulated Cas1-Cas2 in complex with synthesized psDNA sequences psL and psH, which have been measured with low and high efficiency in acquisition, respectively. Notably, such inter-site correlation becomes largely enhanced for Cas1-Cas2 in complex with psH, and remains low with psL. Hence, our studies demonstrate that PAMc recognition and cleavage in one active site of Cas1-Cas2 allosterically regulates non-PAMc association/reaction in the other site, and such allosteric regulation is mediated by non-catalytic Cas2 and DNA protospacer in acquisition. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.18.344481v1?rss=1 Authors: Wang, J. Y., Hoel, C. M., Al-Shayeb, B., Banfield, J. F., Brohawn, S. G., Doudna, J. A. Abstract: CRISPR-Cas systems provide adaptive immunity in bacteria and archaea by targeting foreign DNA for destruction using CRISPR RNA-guided enzymes. CRISPR immunity begins with integration of foreign sequences into the host CRISPR genomic locus, followed by transcription and maturation of CRISPR RNAs. In a few CRISPR systems, the Cas1 integrase and a Cas6 nuclease are fused to a reverse transcriptase that enables viral sequence acquisition from both DNA and RNA sources. To determine how these components work together, we determined a 3.7 [A] resolution cryo-EM structure of a Cas6-RT-Cas1 protein complexed with Cas2, a subunit of the CRISPR integrase. The structure and accompanying mutagenesis experiments provide evidence of bidirectional crosstalk between the Cas1 and RT active sites and unidirectional crosstalk from Cas6 to the Cas1 and RT active sites. Together, these findings suggest regulated structural rearrangements that may coordinate the complex's different enzymatic activities. Copy rights belong to original authors. Visit the link for more info

As we proceed to give you what you need! Next on the #ReadABookPodcast is The Big Payback: The History of the Business of Hip-Hop by Dan Charnas. To discuss this book, Sean Little (host) is joined by a special guest co-host, Cas One of Strange Famous records. On this episode of the Read A Book Podcast Sean and Cas1 break down the beat box era of HipHop from 1984 to 1988 Always connect with the guys by giving your opinion by: 1. Call 470-722-0782 and tell us your story on voicemail. 2. Write your story and add the following tags: #readabookpodcast @iamseanlittle @cas_one @forthdistrict In case you need it again, here's the direct URL to the book: http://amzn.to/2AXBjzB

As we proceed to give you what you need! Next on the #ReadABookPodcast is The Big Payback: The History of the Business of Hip-Hop by Dan Charnas. To discuss this book, Sean Little (host) is joined by a special guest co-host, Cas One of Strange Famous records.On this episode of the Read A Book Podcast Sean and Cas1 break down the beat box era of HipHop from 1984 to 1988Always connect with the guys by giving your opinion by:1. Call 470-722-0782 and tell us your story on voicemail.2. Write your story and add the following tags: #readabookpodcast @iamseanlittle @cas_one @forthdistrictIn case you need it again, here's the direct URL to the book: http://amzn.to/2AXBjzB

As we proceed to give you what you need! Next on the #ReadABookPodcast is The Big Payback: The History of the Business of Hip-Hop by Dan Charnas. To discuss this book, Sean Little (host) is joined by a special guest co-host, Cas One of Strange Famous records. On this episode of RAB, Sean and Cas1 jump into reading "The Big Payback" and break down chapter 2 talking all things hiphop from 1980-84. Always connect with the guys by giving your opinion by: 1. Call 470-722-0782 and tell us your story on voicemail. 2. Write your story and add the following tags: #readabookpodcast @iamseanlittle @cas_one @forthdistrict In case you need it again, here's the direct URL to the book: http://amzn.to/2AXBjzB  

As we proceed to give you what you need! Next on the #ReadABookPodcast is The Big Payback: The History of the Business of Hip-Hop by Dan Charnas. To discuss this book, Sean Little (host) is joined by a special guest co-host, Cas One of Strange Famous records.On this episode of RAB, Sean and Cas1 jump into reading "The Big Payback" and break down chapter 2 talking all things hiphop from 1980-84.Always connect with the guys by giving your opinion by:1. Call 470-722-0782 and tell us your story on voicemail.2. Write your story and add the following tags: #readabookpodcast @iamseanlittle @cas_one @forthdistrictIn case you need it again, here's the direct URL to the book: http://amzn.to/2AXBjzB