Podcasts about mulv

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  • 7PODCASTS
  • 61EPISODES
  • 1h 1mAVG DURATION
  • 1MONTHLY NEW EPISODE
  • Oct 6, 2021LATEST

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Best podcasts about mulv

Latest podcast episodes about mulv

Noisy Neighbors Podcast

RETURN OF THE MAC! Joey is back to join Mulv in the booth and talk all things Manchester City. We discuss the crazy week of big games including Chelsea, PSG, and Liverpool. We wrap up with a segment on Top Tier/Bottom Tier American PL Fans.

Noisy Neighbors Podcast
Restart The Internet

Noisy Neighbors Podcast

Play Episode Listen Later Sep 14, 2021 67:25


Joey and Mulv of the barley mediocre Noisy Neighbors Podcast bring you a review of Manchester City's huge win away at Leicester. We look ahead to the opening match of the Champions League group stage against RB Leipzig, and finish with a listener lead discussion of potential future City CDMs.

Noisy Neighbors Podcast
Izzy Balding

Noisy Neighbors Podcast

Play Episode Listen Later Aug 23, 2021 60:44


Joey and Mulv take the victory lap once again as Manchester City FC get back to winning ways! A review of the Norwich win, a look ahead to Arsenal at home, and a new segment called "Top Tier/Bottom Tier" rounds out the show.www.noisypod.com for all contact , content, and merchandise links

Noisy Neighbors Podcast
UCL Final Post-Mortem

Noisy Neighbors Podcast

Play Episode Listen Later Jun 1, 2021 74:19


Joey and Mulv react to City's disappointing loss and performance against Chelsea in our first Champions League Final.

93:20
THE 9320 MASH UP PODCAST

93:20

Play Episode Listen Later May 26, 2021 72:45


In the ultimate City mash up. Ahsan and Howard are joined by Steven Mcinerney (Esteemed Kompany), David Mooney from the Blue Moon Podcast, Jordan from City Xtra, Andrew Dettmer from the Maine Road Ramble and Joey & Mulv from the Noisy Neighbors to look forward to the Champions League Final.

Noisy Neighbors Podcast
Up Popped An Argentine

Noisy Neighbors Podcast

Play Episode Listen Later May 25, 2021 64:40


Joey and Mulv relive all the weekend's emotions as City finish off the league campaign as Champions, and Sergio Aguero leaves our club as the greatest striker the Premier League has ever seen.

Noisy Neighbors Podcast
It Goes Like This the Fourth the Fifth

Noisy Neighbors Podcast

Play Episode Listen Later May 20, 2021 62:13


Joey and Mulv return after Pep Guardiola wins Manchester City their FIFTH Premier League crown in ten years. Lots of topics today...Ferran Torres, Gabriel Jesus, Harry Kane and Erling Haaland. Also a look at a very strange refereeing performance in Brighton.

Noisy Neighbors Podcast
Basque Sign Language

Noisy Neighbors Podcast

Play Episode Listen Later Apr 26, 2021 60:21


Joey and Mulv are levitating after City win their fourth consecutive League Cup! Of course we look ahead to the big clash with PSG in the Champions League on Wednesday.

Noisy Neighbors Podcast
Quoted a Glazer

Noisy Neighbors Podcast

Play Episode Listen Later Apr 20, 2021 64:17


Joey and Mulv look back at the FA Cup Semi final loss to Chelsea. We also continue the dialogue around the Super League, taking your questions, comments, and emotions.

Bitter and Blue Podcast
53: AguerNOOOOOOO & The Other Guys - feat. Noisy Pod

Bitter and Blue Podcast

Play Episode Listen Later Mar 30, 2021 64:45


Join us, along with our special guest Joey and Mulv of Noisy Neighbors, as we react in the immediate aftermath of the Aguero contract news and take a look at the league through a wide lens of: who is making top 4, who is getting relegated, what players are picking up individual awards, and much more. Were there supposed to be time stamps? Yes. Was the host enjoying this too much to keep track? Also yes.

Noisy Neighbors Podcast

Joey and Mulv review City's 21st consecutive win in all competitions against Wolves! Quick lookbacks at impressive wins over Monchengladbach and West Ham, and rounded off with a preview of this weekend's big Manchester Derby!

Noisy Neighbors Podcast

Joey returns to action to join Mulv in reviewing the tight win against Sheffield United. A little bit of transfer talk kicks off the show, and we finish with a look ahead to matches against Burnley and LiverpoolGet 20% Off and Free Shipping with the code NOISYPOD at Manscaped.com

Noisy Neighbors Podcast
Levitating (feat. LB off City Xtra)

Noisy Neighbors Podcast

Play Episode Listen Later Jan 27, 2021 52:35


Mulv is joined by Lewis Brown from City Xtra to look back at City's big win against West Brom at The Hawthorns. The boys react to listeners' bold proclamations, as well as a preview of City v Sheffield United on Saturday.

Noisy Neighbors Podcast
Juggernaut Creeping Into Gear

Noisy Neighbors Podcast

Play Episode Listen Later Dec 7, 2020 55:21


Joey and Mulv have the Fulham recap for you, as Manchester City complete four clean sheets in a row! We discuss the performance and the encouraging signs as we head into Manchester Derby week. We also look at the Champion League fixture against Marseille on Wednesday as excitement builds for that epic battle lolSupport the show on Patreon for only $1 a month using www.patreon.com/NoisyPod

Noisy Neighbors Podcast

Mulv and McCune recap the big return to form as Manchester City slap Sean Dyche and Burnley! Every player gets a mention as we investigate why City were so much better on Saturday. We then look ahead to Champions League action as The Blues try to seal top spot in the group vs Porto.

Noisy Neighbors Podcast

It's a full episode dedicated to the City v Liverpool match! Joey and Mulv give their opinions on one of the biggest games of the season, and what we can take away from the result and performance!* Get 20% Off + Free Shipping, with the code NOISYPOD at Manscaped.com

Noisy Neighbors Podcast
Mistake-Free Football

Noisy Neighbors Podcast

Play Episode Listen Later Oct 20, 2020 62:19


Joey and Mulv look back City's huge 3 points off Arteta's Arsenal. We discuss tactics and individual performances, then a look ahead to UCL action against FC Porto on Wednesday. The final segment is everybody's favorite "Wanchope's Wheel of Wonders".

Noisy Neighbors Podcast

Mulv and McCune dissect the performance against Leicester City. Joey has the exciting details on City's new centre back Ruben Dias. A quick look ahead to Burnley and Leeds during this very busy week for The Blues!

Noisy Neighbors Podcast
PL Season Preview Part 2

Noisy Neighbors Podcast

Play Episode Listen Later Sep 16, 2020 73:37


Joey and Mulv deliver the promised Season Preview Part 2, with focus on our own Manchester City! We talk thru concerns and confidence boosters ahead of what looks to be a highly competitive season at the top. The lads then pick City's best XI.

Noisy Neighbors Podcast
Net Spend FC

Noisy Neighbors Podcast

Play Episode Listen Later Aug 6, 2020 49:54


Joey and Mulv announce the signings of winger Ferran Torres and defender Nathan Ake. The Eric Garcia drama is legit, and we've got the small matter of Real Madrid in the Champions League to preview.

Noisy Neighbors Podcast
Love At First Sight

Noisy Neighbors Podcast

Play Episode Listen Later Jul 23, 2020 50:08


Joey and Mulv take you down the home stretch of this bizarre season. We look back at the comfortable win against Watford, and the sh*tshow that was the FA Cup Semi Final v Arsenal. We also answer some Listener Questions!

Noisy Neighbors Podcast
Method Man of Didsbury

Noisy Neighbors Podcast

Play Episode Listen Later Jul 10, 2020 61:52


Joey and Mulv are looking back at two contrasting fixtures for City against Newcastle and Southampton. Mad love for David Silva, and a look ahead to Brighton this weekend.

Noisy Neighbors Podcast
Podcast Restart

Noisy Neighbors Podcast

Play Episode Listen Later Jun 14, 2020 56:26


Noisy Neighbors, Joey and Mulv, return to action ahead of Project Restart in the English Premier League! Catch up with the lads big moves during quarantine, as they talk about we can expect from City over the last games of the strangest ever season.

Noisy Neighbors Podcast
Mother's Day Special

Noisy Neighbors Podcast

Play Episode Listen Later May 10, 2020 19:24


It's Mother's Day in the USA, so Mulv calls his Mum for a trip down memory lane.

Noisy Neighbors Podcast
Cornered In A Record Shop

Noisy Neighbors Podcast

Play Episode Listen Later Mar 16, 2020 76:18


With no football on the horizon, Mulv and guest host Howard Hockin discuss the evolution of their musical tastes. Best gigs, guilty pleasures, most overrated bands, the lot.

Noisy Neighbors Podcast
Peroni For The Jabroni

Noisy Neighbors Podcast

Play Episode Listen Later Dec 12, 2019 71:49


Joey welcomes back severely jetlagged Mulv from the Manchester Derby to review the defeat at The Etihad. We also sing the praises of our own Phil Foden after his masterclass in Zagreb. Also includes a quick preview of City's trip to The Emirates this Sunday

Noisy Neighbors Podcast

Mulv & Joey recap the contrasting performances away to Newcastle and Burnley. The lads are also gearing up for Manchester Derby weekend, and Mulv hopes not to jinx it again when he visits The Etihad.

Noisy Neighbors Podcast
Fire Up Your Leaf Blowers

Noisy Neighbors Podcast

Play Episode Listen Later Nov 20, 2019 70:09


Joey and Mulv are joined by City fan, author, and podcast extraordinaire Howard Hockin! We talk big news items, break out Wanchope's Wheel of Wonders, and of course there's a quiz.

For The Love of Paul McGrath: An Aston Villa Podcast
Man City Preview with Joey & Mulv of @NoisyPod

For The Love of Paul McGrath: An Aston Villa Podcast

Play Episode Listen Later Oct 24, 2019 55:27


Man City Preview with Joey & Mulv of @NoisyPod Great chat about Citys form this season - Apple Podcast http://apple.co/2Z5aRiH- Spotify http://spoti.fi/2KxEzoC& on all Podcast apps Ads: @BigHeadsMedia@bridgetcase_ (After Orange Slices) #PodernFamily #AVFC #PartOfThePride

Manc and a Yank Podcast
Episode 12 - The Real Fourmidables feat. NoisyPod

Manc and a Yank Podcast

Play Episode Listen Later Oct 3, 2019 54:19


Jon and Joe are joined by City podcast royalty as the Noisy Neighbors pop in to review the wins against Dinamo Zagreb and Everton. Joe and Mulv tuck in to Mahrez and Ederson-flavored humble pies, Joey fawns over the Iberian Thanos, and there’s a serious debate over who would make it into City’s strongest eleven.

Noisy Neighbors Podcast
The Rebound

Noisy Neighbors Podcast

Play Episode Listen Later Sep 25, 2019 72:27


Joey and Mulv recap a stunning week of football from Champions Manchester City. We review three big results across three competitions, as the Blues respond to the surprise loss away to Norwich.

Noisy Neighbors Podcast
You Might Win Some

Noisy Neighbors Podcast

Play Episode Listen Later Sep 18, 2019 65:54


Mulv and Joey review an awful weekend as City lose away to Norwich. The Champions League is back, as we preview the trip to Ukraine. We also respond to some of your assumptions about the show.

Noisy Neighbors Podcast
Top Bins McCune

Noisy Neighbors Podcast

Play Episode Listen Later Aug 27, 2019 59:13


Mulv and Joey return with a review of City's 3-1 win away to Bournemouth this weekend. We also look ahead to Brighton's visit to Stade De Etihad, and answer a couple of listener questions.

Noisy Neighbors Podcast
Here's To You

Noisy Neighbors Podcast

Play Episode Listen Later May 7, 2019 51:22


Joey and Mulv re-live the drama of City's huge win against Leicester. Big love for the heroic effort from skipper Vincent Kompany, and The Blues are just one result away from back to back Premier League titles!

Noisy Neighbors Podcast
The Old Guard

Noisy Neighbors Podcast

Play Episode Listen Later May 2, 2019 60:31


Mulv and McCune back in the studio as this potentially historic season winds down. We've got a review of the very tense match away to Burnley, and a look ahead to the crucial visit of Leicester to The Etihad on Monday night. We also give our thoughts on the results of the recent Player Awards!

Noisy Neighbors Podcast
Spoils Of The Conquered

Noisy Neighbors Podcast

Play Episode Listen Later Apr 24, 2019 72:16


Joey n Mulv are giddy with joy as Man City defeat formerly decent side Man United in the Manchester Derby. Immediate match reaction, followed by a Champions League post-mortem, as City failed to get by Spurs in the quarter-final.

Noisy Neighbors Podcast
We're Going For It

Noisy Neighbors Podcast

Play Episode Listen Later Apr 16, 2019 61:12


Joey and Mulv react to the big win at Selhurst Park! There's big Champions League Quarter Final discussion as City take on Spurs, and we finish with your Star Wars themed questions from Twitter. Amazing.

Noisy Neighbors Podcast

Mulv is joined by Jon Ashley and Joe Ricci of the Great Northeast, to discuss the fresh result over Cardiff, the performance against Fulham, and a look ahead to Wembley this Saturday!

Noisy Neighbors Podcast
By The Balls

Noisy Neighbors Podcast

Play Episode Listen Later Mar 28, 2019 62:53


Joey McCune returns to join yer man Mulv, as these two get outlandish for your International Break. We recap the international fixtures and the City players involved. We look ahead to Fulham this weekend, and answer some listener questions!

Noisy Neighbors Podcast

Mulv is joined by City blogger Joe Ricci (@ViewsOfABlue) to discuss the Swansea result, as well as some transfer talk during this international break. They also answer some listener questions!

Noisy Neighbors Podcast
Who's Got The Bottle

Noisy Neighbors Podcast

Play Episode Listen Later Mar 5, 2019 56:13


Joey and Mulv review City's 1-0 away win against Bournemouth, and take a look at the final stretch of the Premier League title race. We have a quick look at all four competitions, including a preview of Watford at home on Saturday. Enjoy!

Noisy Neighbors Podcast
Hit For Six

Noisy Neighbors Podcast

Play Episode Listen Later Feb 12, 2019 62:34


Mulv and McCune back on the scene reviewing the magnificent performance versus Chelsea at Stade de Etihad. We look ahead to Newport in the FA Cup, and answer a couple of listener questions!

Noisy Neighbors Podcast

Mulv and Joey are back in studio in the middle of a huge week for Man City! Everton and Arsenal reviews, along with a preview of City v Chelsea, as our boys seek revenge for the loss at Stamford Bridge earlier this season.

Noisy Neighbors Podcast

Joey returns to join Mulv in reviewing City's defeat at St. James Park. We also look back at the FA Cup triumph v Burnley, and ahead to the big match at home to Arsenal on Sunday. Enjoy, Blues!

Noisy Neighbors Podcast
We're Off To Wembley!

Noisy Neighbors Podcast

Play Episode Listen Later Jan 24, 2019 67:40


Mulv is joined by friends of the show Jon Ashley and Nico Morales, as frontman Joey recovers from the flu. We review the League Cup Semi away to Burton Albion, Huddersfield Away, and answer some listener questions. Enjoy!

Noisy Neighbors Podcast

Host Joey "McClune" is on the sick this week, so Mulv is joined by old friend Walter Smith (BoltFromABlue Pod, Blue Moon Rising TV) - We talk Wolves, the big result against Burton Albion, and look ahead to Huddersfield Away this weekend!

Noisy Neighbors Podcast

Mulv and Joey review the momentum changing victory over Liverpool at The Etihad! We also touch quickly on the FA Cup result against Rotherham, and look ahead to Wolves on Monday. Enjoy!

Noisy Neighbors Podcast

Joey and Mulv are back after the Christmas period, and we're ringing in the New Year! BIG show with a Liverpool Preview, and a review of Southampton Away. We also look at the many reasons City dropped points in the title race.

Noisy Neighbors Podcast
Next Man Up

Noisy Neighbors Podcast

Play Episode Listen Later Nov 28, 2018 67:32


Mulv and Joey bring the hottest takes on the big Champions League draw with Lyon. We're also looking back on the entertaining victory at West Ham, and a quick look at this weekend versus Bournemouth at The Etihad. Vamos!

Noisy Neighbors Podcast
Fake Friendlies

Noisy Neighbors Podcast

Play Episode Listen Later Nov 20, 2018 43:44


McCune and Mulv are back on the podcast during International Break! We preview the visit to West Ham this weekend. We've got Wanchope's Wheel Of Wonders, and a question from the Twitter. Let's go!

Noisy Neighbors Podcast
Manchester Mismatch

Noisy Neighbors Podcast

Play Episode Listen Later Nov 13, 2018 64:05


Mulv n McCune are back in studio reviewing the lop-sided Manchester Derby, featuring Mourinho's Midtable Mercenaries. Raheem Sterling contract chatter, and listener questions round out this week's show!

Noisy Neighbors Podcast
David Silva Olé

Noisy Neighbors Podcast

Play Episode Listen Later Oct 24, 2018 59:22


Joey and Mulv review City's impressive Champions League performance away to Shakhtar. There's some listener questions from our email bag, and a look ahead to the big match against Spurs at Wembley!

93:20
The Noisy Review - Red Hot

93:20

Play Episode Listen Later Oct 21, 2018 63:45


A VERY special 93:20 review podcast with our favourite Stateside podcasters Noisy Neighbours aka Joey and Mulv joining host Ahsan to talk through the evisceration of Burnley at the Etihad. Normally this would be a #9320player show, but as it's a SPECIAL, we're sharing it with everyone! Up the Noisy Blues!

Noisy Neighbors Podcast
Bananas In Pyjamas

Noisy Neighbors Podcast

Play Episode Listen Later Oct 18, 2018 50:33


Mulv and Joey are joined by far-flung blue Patrick Holley from Sydney Australia! The boys talk about City's season so far, as well as other title challengers. Raheem Sterling is on the agenda. We answer listener questions and also preview Burnley @ Home this weekend. Get stuck in!

Noisy Neighbors Podcast
Live From Johnny Marr's Tour Bus

Noisy Neighbors Podcast

Play Episode Listen Later Oct 10, 2018 55:12


Starstruck Joey and Mulv are joined by guitar legend, Johnny Marr (The Smiths, Electronic, Modest Mouse et al.) We review the draw at Anfield and hear other insight about Manchester City from the lifelong Blue!

Noisy Neighbors Podcast
Old Man Corner

Noisy Neighbors Podcast

Play Episode Listen Later Sep 4, 2018 63:55


Mulv and Joey look back at the win and performance versus Newcastle. Predictions for individual awards in this season's Premier League. Wanchope's Wheel Of Wonders returns!

Noisy Neighbors Podcast
Bad Day At The Office

Noisy Neighbors Podcast

Play Episode Listen Later Aug 30, 2018 66:25


Your boys Joey and Mulv are reviewing all the draws. Wolves, Champions League and Carabao Cup. We also preview the match against Newcastle this weekend in the Premier League!

Noisy Neighbors Podcast

The Noisy Neighbors are back! This week, Mulv and Joey discuss City's Community Shield win, give their top 4 predictions, and preview the Arsenal match this Sunday.

Noisy Neighbors Podcast
Summer Recap

Noisy Neighbors Podcast

Play Episode Listen Later Aug 8, 2018 70:50


The Noisy Neighbors are back!! After a long summer, Mulv and Joey are back in the booth and ready to talk all things City. The boys look back at the World Cup, City's transfer dealings, the summer tour and preview the upcoming Community Shield match against Chelsea!

SAGE Veterinary Science
TPX October 2015: Immunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

SAGE Veterinary Science

Play Episode Listen Later Oct 21, 2015 27:49


This podcast with Dr. Jerold Rehg and hosted by Dr. Odete Mendes discusses the classification  of mice hematolymphoid tumors in CD1 mice, their pattern of incidence in regards to the age, sex or feeding status and their morphology, cell composition, organ involvement.  Immunohistochemical (IHC) markers that are diagnostically relevant  for these tumors were also discussed.  Dr Rehg characterized the cellular lineage of spontaneous hematolymphoid neoplasms arising in  female CD-1 mice.  Lymphoblastic lymphomas of T-cell and B-cell lineage were common in mice 12 months or less of age, whereas a wide range of non-lymphoblastic B-cell lymphomas and lymphoblastic T-cell lymphomas were common in mice older than 12 months. Additionally, he discussed the presence of renal hyaline droplets positive for lysozyme observed in aged mice with histiocytic-associated large B-cell lymphomas and myeloid leukemia. Finally, Dr Rehg also discussed the recovery of endogenous ecotropic MuLV genes from CD-1 mice and the expression of MuLV protein by IHC in lymphomas and some normal tissues of both young and aging CD-1 mice.  The data and methodology described by Dr Rehg will help differentiate and characterize spontaneous lymphomas and leukemias in CD-1 mice. The methodology described is also very relevant for broader immunophenotyping of genetically modified mice and drug immunomodulatory effects.   Click here to read the full article.

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06
Entwicklung lentiviraler Transgenese in höheren Säugetieren

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06

Play Episode Listen Later Apr 7, 2006


Die ersten transgenen Tiere wurden durch viralen Gentransfer erzeugt. Für die initialen Versuche wurden prototypische Retroviren, wie der murine Leukämievirus (MuLV), verwendet. Es stellte sich jedoch heraus, daß die proviralen Gene in diesen Mäusen stark methyliert waren und nicht oder nur in geringen Mengen exprimiert wurden ("gene silencing"). Ein Durchbruch für die virale Transgenese kam erst mit der Verwendung lentiviraler Vektoren. Lentiviren sind in der Lage eine Vielzahl verschiedener Zelllinien (auch terminal differenzierte Zellen) effizient zu transduzieren und ihre virale DNA stabil in das Wirts-Chromosom zu integrieren. Obwohl bereits transgene Nagetiere durch lentivirale Vektoren erzeugt werden konnten, waren initiale Versuche in höheren Säugetieren (Affen) nicht erfolgreich. Dies warf die Frage auf, ob lentiviraler Gentransfer in höheren Säugetieren anwendbar ist. Transgene Schweine und Rinder wären von großer biomedizinischer Bedeutung. Ihre potentiellen Anwendungsmöglichkeiten reichen von der Produktion pharmazeutisch relevanter Proteine über klinische Modelle zur Untersuchung humaner Erkrankungen bis hin zur Xenotransplantation. Obwohl mit der klassischen DNA-Mikroinjektion transgene Schweine und Rinder erzeugt werden können, ist das Verfahren in diesen Spezies jedoch sehr ineffizient und dementsprechend kostenintensiv. Da hohe Produktionskosten den möglichen Anwendungen entgegenstehen, wurde versucht ein effizientes Verfahren, daß auf lentiviralem Gentransfer beruht, zu entwickeln. Für die Entwicklung der lentiviralen Transgenese in Schweinen wurden Zygoten mit Lentiviren infiziert und in Empfänger transferiert. Die verwendeten Vektoren trugen einen eGFP-Reporter, um die Effizienz der Transduktion schnell und einfach beurteilen zu können. Von den 46 geborenen Ferkeln waren 32 transgen und 30 zeigten Transgen-Expression (65%). Die hohe Transgenese-Rate, die mit dem lentiviralen Gentransfer erreicht werden konnte, stellt eine 27fache Steigerung der Effizienz im Vergleich zur klassischen DNA-Mikroinjektion dar. Die Untersuchung der transgenen Ferkel zeigte Transgen-Expression in allen Organe und keinen sichtbaren Mosaicismus der F0-Tiere. Des weiteren konnte eine nahezu lineare Korrelation zwischen der Anzahl der integrierten Proviren und der Höhe der Transgen-Expression gezeigt werden. Die Expression der lentiviralen Transgene war stabil und wurde nicht nach der Geburt der Tiere abgeschaltet. Durch die Wahl geeigneter Promotoren war es möglich sowohl ubiquitäre, als auch Gewebe-spezifische Expression (in der Haut) zu erreichen. Die integrierten Proviren wurden über die Keimbahn an die nächste Generation weitergegeben und in der F1-Generation unverändert stark exprimiert. Die Weitergabe der integrierten Proviren an die nächste Generation ist die Basis für Erzeugung transgener Linien. Zur Erzeugung transgener Rinder wurden initial ebenfalls Zygoten infiziert. Diese wurden in vitro bis zum Blastozysten-Stadium (Tag 7) kultiviert. Überraschenderweise zeigten die Blastozysten nur sehr geringe Transgen-Expression. Nachdem durch Transfer solcher Blastozysten keine transgenen Nachkommen erzeugt werden konnten, wurde zur Infektion von Oozyten (vor der Befruchtung) gewechselt. In den aus Oozyten-Infektion stammenden Blastozysten war die Gentransfer-Rate wesentlich höher (insgesamt 83% eGFP+ Blastozysten) und die eGFP-Fluoreszenz um ein Vielfaches intensiver. Acht eGFP-positive Blastozysten wurden in vier Empfänger transferiert, was zur Geburt von vier transgenen Rindern führte. Alle erzeugten transgenen Rinder zeigten stabile Expression des Transgens in allen untersuchten Organen. Als eine weitere Methode zur Erzeugung lentiviral transgener Rinder wurde der Kerntransfer (NT) untersucht. Hierzu wurden Haut-Fibroblasten vom Rind lentiviral transduziert und als Donor-Zellen verwendet. Dieser Ansatz war zwar wesentlich ineffizienter als die direkte Infektion von Oozyten, trotzdem konnte ein transgenes Rind erzeugt werden, das starke Transgen-Expression zeigte. Da die Expression lentiviraler Integranten offenbar durch das klassische Klonen nicht abgeschaltet wird, eröffnet diese Methode viele Möglichkeiten für die Produktion transgener Tiere. Im letzten Teil dieser Arbeit wurde die epigenetische Regulation lentiviraler Vektoren untersucht. Dazu wurden transgene Founder-Schweine verpaart, um Tiere mit einzelnen lentiviralen Integranten (F1-Generation) zu erzeugen. Die Expressions-Analyse dieser Schweine zeigte, daß etwa 1/3 der Proviren nur schwach bzw. gar nicht exprimierten. Durch Southern Blot Analysen mit Methylierungs-sensitiven Restriktions-Enzymen wurde der Grad der proviralen Methylierung bestimmt. Dieser korrelierte negativ mit der Transgen-Expression. Zur genaueren Analyse der Methylierungs-Dichte wurden die verschiedenen Proviren mittels Bisulfit-Sequenzierung untersucht. Es stellte sich heraus, daß in den schwach bzw. nicht-exprimierenden Integranten nahezu alle CpG-Dinukleotide innerhalb der untersuchten Sequenzen methyliert waren. Um den Einfluß der Methylierung auf die Expression zu untersuchen, wurde von einem nicht-exprimierenden Schwein Haut-Fibroblasten isoliert und mit dem Methylase-Inhibitor 5-AzaC inkubiert. Dadurch konnte die abgeschaltete eGFP-Expression wieder reaktiviert werden. Dagegen hatte der Histon-Deacetylase Inhibitor TSA keinen starken Einfluß auf die Transgen-Expression. Chromatin-Modifikationen durch TSA-abhängige HDACs scheinen also bei der epigenetischen Regulation lentiviraler Vektoren in Schweinen keine entscheidende Rolle zu spielen. Abschließend konnte durch einen Methylierungs-sensitiven Southern Blot gezeigt werden, daß der Grad der DNA-Methylierung durch Hemmung zellulärer Methylasen (mit 5-AzaC) signifikant reduziert wurde. Lentiviraler Gentransfer stellte sich als eine sehr effiziente Methode zur Erzeugung transgener Schweine und Rinder heraus. Das Verfahren zeichnet sich insbesondere durch hohe Transgenese-Raten und hohe Transgen-Expression aus. Außerdem werden die lentiviralen Integranten über die Keimbahn an die nächste Generation weitergegeben. Obwohl die Transkription einiger Proviren epigenetisch reguliert wurde, ist die Häufigkeit des aufgetretenen Silencings deutlich geringer als bei prototypischen Retroviren.