Podcasts about three dimensional imaging

This week on The Beat, CTSNet Editor-in-Chief Joel Dunning dives into the best resources available for cardiothoracic surgery professionals. He highlights key websites, including The Society of Thoracic Surgeons (STS), the European Association for Cardio-Thoracic Surgery (EACTS), The American Association for Thoracic Surgery (AATS), and CTSNet, offering valuable insights into each platform's offerings. Joel also explores some lesser known yet valuable resources, such as the American Heart Association (AHA), YouTube, Vimeo, and Doximity. Additionally, he discusses the groundbreaking achievement of the world's first robotic cardiac telesurgery, performed by Sudhir Srivastava of SS Innovations.   Joel also reviews recent JANS articles on the presentation and outcomes of women and men undergoing surgery for degenerative mitral regurgitation, surgical aortic annulus enlargement with a balloon catheter, lung transplant outcomes in recipients of advanced age, and the impact of three-dimensional imaging and printing on septal myectomy results.  In addition, Joel explores a mitral valve replacement for rheumatic heart disease through an upper hemisternotomy, an inclusion Ross procedure with hemiarch repair using antegrade cerebral perfusion, and an interview with Dr. Ali Dodge-Khatami on minimal invasive repairs of congenital heart defects. Before closing, he highlights upcoming events in CT surgery.   JANS Items Mentioned  1.) Presentation and Outcomes of Women and Men Undergoing Surgery for Degenerative Mitral Regurgitation  2.) Dealing With the Aortic Annulus: Surgical Aortic Annulus Enlargement With a Balloon Catheter   3.) Lung Transplant Outcomes in Recipients of Advanced Age: Are Two Always Better Than One?  4.) Impact of Three-Dimensional Imaging and Printing on Septal Myectomy Results—Single Centre's Experience  CTSNET Content Mentioned  1.) A Mitral Valve Replacement for Rheumatic Heart Disease Through an Upper Hemisternotomy  2.) Inclusion Ross Procedure With Hemiarch Repair Using Antegrade Cerebral Perfusion: The Modified Starnes Technique  3.) Minimal Invasive Repairs of Congenital Heart Defects: An Interview With Ali Dodge-Khatami  Other Items Mentioned  CTSNet Events Calendar  Disclaimer The information and views presented on CTSNet.org represent the views of the authors and contributors of the material and not of CTSNet. Please review our full disclaimer page here.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.28.521740v1?rss=1 Authors: Kanatani, S., Kreutzmann, J. C., Li, Y., West, Z., Vougesi Nikou, D., Lercke Skytte, J., Lydolph Larsen, L., Tanaka, D. H., Kaczynska, D., Fukumoto, K., Uesaka, N., Tanabe, T., Miyakawa, A., Roostalu, U., Hecksher-Sorensen, J., Uhlen, P. Abstract: Whole-brain three-dimensional (3D) imaging is desirable to obtain a comprehensive and unbiased view of architecture and neural circuitry. However, current spatial analytic methods for brain RNAs are limited to thin sections or small samples. Here, we combined multiple new techniques to develop TRIC-DISCO, a new pipeline that allows imaging of RNA spatial distributions in whole adult mouse brains. First, we developed Tris-mediated retention of in situ hybridization signal during clearing (TRIC), which produces highly transparent tissue while maintaining the RNA signal intensities. We then combined TRIC with DISCO clearing (TRIC-DISCO) by controlling temperature during the in situ hybridization chain reaction (isHCR) to ensure uniform whole-brain staining. This pipeline eliminates the requirements for both strict RNase-free environments and workflow-compatible RNase inhibitors. Our TRIC-DISCO pipeline enables simple and robust, single-cell, whole-brain, 3D imaging of transcriptional signatures, cell-identity markers, and noncoding RNAs across the entire brain. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.30.220541v1?rss=1 Authors: Findlay, R. C., Osman, M., Spence, K., Kaye, P. M., Walrad, P. B., Wilson, L. G. Abstract: Cellular motility is an ancient eukaryotic trait, ubiquitous across phyla with roles in predator avoidance, resource access and competition. Flagellar-dependent motility is seen in a variety of parasitic protozoans and morphological changes in flagellar structure and function have been qualitatively described during differentiation. However, whether the dynamics of flagellar motion vary across lifecycle stages and whether such changes serve to facilitate human infection is not known. Here we used holographic video microscopy to study the pattern of motility in insect midgut forms of Leishmania (procyclic promastigotes; PCF) and differentiated human infective metacyclic promastigotes (META). We discovered that PCF swim in a slow, corkscrew motion around a gently curving axis while META display run and tumble behaviour in the absence of stimulus, reminiscent of bacterial behaviour. In addition, we demonstrate that META specifically respond to a macrophage-derived stimulus, modifying swimming direction and speed to target host immune cells. Thus, the motility strategy employed by Leishmania appears as a random search that is replaced with a ballistic swimming motion in the presence of an immunological stimulus. These findings shed unique insights into how flagellar motion adapts to the particular needs of the parasite at different times in its lifecycle and define a new pre-adaptation for infection of the human host. Copy rights belong to original authors. Visit the link for more info

The PRS Journal Club and special guest moderator Dr. Debra Johnson to discuss the following articles from the June 2017 issue: “Breast Cancer after Augmentation: Oncologic and Reconstructive Considerations among Women Undergoing Mastectomy” by Hollenbeck et al. "Early post-operative results of percutaneous needle fasciotomy in 451 patients with Dupuytren disease” by Molenkamp et al. "Pursuing Mirror Image Reconstruction in Unilateral Microtia: Customizing Auricular Framework by Application of Three-Dimensional Imaging and Three-Dimensional Printing” by Chen et al.

Dr. Louis Kaufman is a nationally recognized educator, author and practicing clinician.  He is a graduate of the University Of Illinois School Of Dentistry.  Being highly experienced with LUMINEERS®, he has successfully completed over 1,000 patient cases in the Chicago area and continues to tour the country to train and educate other dental professionals about LUMINEERS® and other major dental procedures.  In 1995, he joined his father Richard’s well-established 60-year-old General Dentistry practice treating 3rd and 4th generation patients.  Dr. Kaufman serves on the advisory board of numerous dental manufacturers, consults on product development and educates clinicians around the globe.  He lectures at approximately 20 continuing education programs annually and has published numerous articles focused on restorative and cosmetic dentistry. Dr. Kaufman holds professional memberships in The American Dental Association, The Academy of General Dentistry, The American Academy of Cosmetic Dentistry, and The Chicago Dental Society, The American Academy of Dental Sleep Medicine, The Academy of Laser Dentistry and Illinois State Dental Society.  Areas Of Special Interest: LUMINEERS, IMPLANTS, Cosmetic Smile Design and Three Dimensional Imaging.

An introduction to the 2014 review by Cristina Risco, Isabel Fernandez de Castro, Laura Sanz-Sanchez, Kedar Narayan, Giovanna Grandinetti, and Sriram Subramaniam, "Three-Dimensional Imaging of Viral Infections," from the Annual Review of Virology.

How it works and what it can do. A close look at it's probe, using an electron microscope.

Transcript -- How it works and what it can do. A close look at it's probe, using an electron microscope.

How it works and what it can do. A close look at it's probe, using an electron microscope.

Transcript -- How it works and what it can do. A close look at it's probe, using an electron microscope.