Podcasts about single cell

New research points to a rare cosmic event just 40 years ago that changes what we know about Uranus' magnetosphere. Also, scientists create a living, breathing mouse using ancient single-cell genes. Plus, on 'This Day in History'; smoking is banned on domestic flights in the US. Voyager 2's defining Uranus flyby may have been skewed by a rare cosmic event | CNN We've Only Been To Uranus Once And The Freak Timing May Have Misled Us For Years The anomalous state of Uranus's magnetosphere during the Voyager 2 flyby | Nature Astronomy Scientists recreate mouse from gene older than animal life Twenty-five Years Ago, U.S. Airlines Banned Smoking On Domestic Flights TDIH: Celebrating 25 Years of No Smoking in Airplanes – SEATCA Contact the show - coolstuffcommute@gmail.com Learn more about your ad choices. Visit megaphone.fm/adchoices

Nels and Vincent review the identification of a novel virus associated with the pathogen Plasmodium knowlsei and which is part part of a diverse and unclassified viral taxon. Hosts: Nels Elde and Vincent Racaniello Subscribe (free): Apple Podcasts, RSS, email Become a patron of TWiEVO Links for this episode Join the MicrobeTV Discord server Virus associated with P. knowlsei (bioRxiv) Timestamps by Jolene Science Picks Nels – The peace of wild things, poem by Wendell Berry Vincent – All Life on Earth Today Descended From a Single Cell. Meet LUCA Music on TWiEVO is performed by Trampled by Turtles Send your evolution questions and comments to twievo@microbe.tv

Caroline Alan is a health survivor and mineral enthusiast. Coming from a career in the corporate world, Caroline found herself struggling physically, mentally and energetically.  During her journey back to health, Caroline encountered plant-based minerals and their incredible ability to support a return to natural balance in the body.  As a result, she has become devoted to educating people about the benefits of mineral replenishment and the specific efficacy of plant-based humic and fulvic substances.   Caroline's research into why minerals are important in human physiology and how they work in the body, has taken her deep into microbiology, molecular biology, cellular biology, agricultural soil science and the study of plant decomposition. In the process, Caroline has found ways to help others understand the effects of mineral depletion and why plant-based humic and fulvic mineral supplementation support healing and optimal health.   Caroline is the Co-Founder & CEO of BEAM Minerals, which provides liquid humic and fulvic mineral supplements. “These humic and fulvic complexes, they're like Mother Nature's answer to mineral supplementation,” Caroline says.

In this episode of FYI, Brett Winton, ARK's Chief Futurist, and Nemo Marjanovic, ARK's Multiomics Analyst, have an in-depth discussion with Professor Nikolai Slavov from Northeastern University. Professor Slalov is the Director of Parallel Squared Technology Institute (PTI). Together they explore the fascinating and complex world of multiomics, focusing on the importance of proteomics in understanding biological processes. Professor Slavov shares his insights on the technological advancements in single-cell proteomics, the challenges in measuring protein interactions, and the critical role of proteomics in drug development. The conversation also delves into the underfunded nature of proteomics research and its potential to revolutionize biopharma R&D and diagnostics.Key Points From This Episode:Introduction to multi-omics and its relevance in modern biology.Explanation of the central dogma of molecular biology: DNA, RNA, and proteins.Overview of Professor Slavov's research on single-cell proteomics and its implications.Challenges in measuring the dynamic and complex nature of the proteome.The role of advanced technologies like mass spectrometry in proteomic analysis.Importance of protein interactions and modifications in cellular functions.Discussion on the underfunding of proteomics research and its impact on scientific progress.The potential of proteomics to improve drug development and diagnostic precision.Advances in AI and data analysis techniques for better understanding protein structures and functions.Future frontiers in proteomics, including improved technology access and dynamic protein measurements.References:MacCoss, M.J., Alfaro, J.A., Faivre, D.A. et al. Sampling the proteome by emerging single-molecule and mass spectrometry methods. Nat Methods 20, 339–346 (2023). https://doi.org/10.1038/s41592-023-01802-5 Derks J, Jonson T, Leduc A, Khan S, Khoury L, Rafiee M, Slavov N Single-nucleus proteomics identifies regulators of protein transportbioRxiv  doi: 10.1101/2024.06.17.599449 (2024)

Relation leverages single-cell multi-omics, functional assays, and machine learning to better characterize diseases and develop transformational medicines.

This week on Data in Biotech, we are joined by Parul Bordia Doshi, Chief Data Officer at Cellarity, a company that is leveraging data science to challenge traditional approaches to drug discovery.  Parul kicks off the conversation by explaining Cellarity's mission and how it is using generative AI and single-cell multiomics to design therapies that target the entire cellular system, rather than focusing on single molecular targets. She gives insight into the functionality of Cellarity Maps, the company's cutting-edge visualization tool that maps the progression of disease states and bridges the gap between biologists and computational scientists.  Along with host Ross Katz, Parul walks through some of the big challenges facing Chief Data Officers, particularly for biotech organizations with data-centric propositions. She emphasizes the importance of robust data frameworks for validating and standardizing complex data sets, and looks at some of the practical approaches that ensure data scientists can derive the maximum amount of value from all available data.  They discuss what data science teams look like within Cellarity, including the unique way the company incorporates human intervention into its processes. Parul also emphasizes the benefits that come through hiring multilingual, multidisciplinary teams and putting a strong focus on collaboration.  Finally, we get Parul's take on the future of data science for drug discovery, plus a look at Cellarity's ongoing collaboration with Novo Nordisk on the development of novel therapeutics.  Data in Biotech is a fortnightly podcast exploring how companies leverage data innovation in the life sciences. Chapter Markers [1:45] Introduction to Parul, her career journey, and Cellarity's approach to drug discovery. [5:47] The life cycle of data at Cellarity from collection to how it is used by the organization.  [7:45] How the Cellarity Maps visualization tool is used to show the progression of disease states [9:05] The role of a Chief Data Officer in aligning an organization's data strategy with its company mission.   [11:46] The benefits of collaboration and multidisciplinary, cross-functional teams to drive innovation.  [14:53] Cellarity's end-to-end discovery process; including how it uses generative AI, contrastive learning techniques, and visualization tools.  [19:42] The role of humans vs the role of machines in scientific processes.  [23:05] Developing and validating models, including goal setting, benchmarking, and the need for collaboration between data teams and ML scientists. [30:58] Generating and managing massive amounts of data, ensuring quality, and maximizing the value extracted. [37:08] The future of data science for drug discovery, including Cellarity's collaboration with Novo Nordisk to discover and develop a novel treatment for MASH.

Part 3 of 4. My guest for this week's episode is Noam Solomon, CEO and co-founder at Immunai, a pioneering biotech company that is comprehensively mapping and reprogramming the immune system with single-cell biology and AI to power new therapeutic discoveries, accelerate drug development, and improve patient outcomes.

In this week's episode of the Everything Epigenetics podcast, Dr. Hannah Went sits down with Alex Trapp, a computational biologist deeply involved in aging research. The two explore the cutting-edge development of single-cell epigenetic clocks and their potential applications in the field of longevity science. Alex shares his journey from experimental biology to computational approaches and discusses the significant implications of understanding aging at the single-cell level. He also provides insights into his work at Retro Biosciences, a company focused on extending healthy human lifespan through innovative therapies. In this episode of Everything Epigenetics, you'll learn about: • The development and significance of single-cell epigenetic clocks • Differences between bulk tissue and single-cell epigenetic sequencing methods • Applications of single-cell aging clocks in understanding and potentially reversing aging • Challenges and future directions in single-cell epigenetic research • The exciting work being done at Retro Biosciences, including advancements in T-cell rejuvenation and plasma therapeutics Chapters: 00:00 Introduction 03:15 Alex Trapp's journey in the aging research field 07:35 Overview of epigenetic clocks and single-cell approaches 17:38 Development and challenges of the single-cell aging clock 22:41 Applications and implications of single-cell clocks in aging research 35:57 Future directions in epigenetic research 42:55 Alex's work at Retro Biosciences and its potential impact Review Alex's Study: https://pubmed.ncbi.nlm.nih.gov/36211119/ Support the Show: https://www.buzzsprout.com/2084028/support Thank you for joining us at the Everything Epigenetics Podcast, and remember, you have control over your epigenetics, so tune in next time to learn more about how. Support the Show.Thank you for joining us at the Everything Epigenetics Podcast and remember you have control over your Epigenetics, so tune in next time to learn more about how.

Send us a Text Message.Curious about the latest innovations in cell analysis technology? Join us in this episode with Furkan Gökçe, New Business Development Manager at Amphasys, as we delve into the fascinating world of Impedance Flow Cytometry (IFC).Furkan shares how IFC is revolutionizing single-cell analysis by providing faster, more precise, and high-resolution data.Key Takeaways:Real-Time Precision: Discover how IFC allows for real-time, label-free assessments of cells, providing detailed insights into their natural state.Enhanced Process Control: Learn how IFC can detect early signs of nutrient deficiencies and contaminants, leading to timely interventions and improved bioreactor outcomes.Versatile Applications: Hear about practical applications, from brewing to protein production, where IFC has significantly boosted yields and process efficiency.Tune in to uncover how IFC is set to transform the landscape of bioprocessing, ensuring better quality and sustainability in biologics production.Connect with Furkan Gökçe:LinkedIn: https://www.linkedin.com/in/furkangokceAmphasys: https://amphasys.com/bioprocessingNext Steps:Book a free assessment to help you get started with the implementation of new technologies in your manufacturing processes: https://bruehlmann-consulting.comDevelop biologics better, faster, at a fraction of the cost with our Fractional CTO services. Curious? DM us at hello@bruehlmann-consulting.com

Send us a Text Message.Ever wondered how single-cell analysis could revolutionize bioprocessing? Join us in this episode as we delve into the cutting-edge world of impedance flow cytometry.Furkan Gökçe, New Business Development Manager at Amphasys, shares his insights on the transformative power of this technology in the biotech industry. With a background in engineering and physics, Furkan provides a unique perspective on tackling the complexities of biological systems.Key Takeaways:Enhanced Process Control: Learn how impedance flow cytometry offers high-resolution, label-free analysis of individual cells, ensuring optimal growth conditions and quality production.Understanding Cell Heterogeneity: Discover the importance of single-cell analysis in revealing critical variations within cell populations that bulk measurements often miss.Innovation in Bioprocessing: Explore the advancements in microfabrication techniques that make real-time, high-throughput cell analysis possible, revolutionizing bioprocessing efficiency and accuracy.Tune in to gain valuable knowledge on how this innovative technology can enhance your bioprocess development and manufacturing strategies!Connect with Furkan Gökçe:LinkedIn: https://www.linkedin.com/in/furkangokceAmphasys: https://amphasys.com/bioprocessingNext Steps:Book a free assessment to help you get started with the implementation of new technologies in your manufacturing processes: https://bruehlmann-consulting.comDevelop biologics better, faster, at a fraction of the cost with our Fractional CTO services. Curious? DM us at hello@bruehlmann-consulting.com

See omnystudio.com/listener for privacy information.

The body of 27-year-old mother of two, Samantha Bodsworth, was found in Noosa, surrounded by wattle trees. The killer thinks it's the perfect crime, but what they underestimated was the power of the tiny, silent witnesses clinging to almost everything they touched – pollen. In this episode, Dr Lynne Milne, the scientist at the forefront of forensic palynology, reveals how, for the first time in an Australian criminal case, pollen helped unmask a murderer. This episode references men's violence against women. If you or someone you know is experiencing, or at risk of experiencing, domestic, family or sexual violence, call 1800RESPECT on 1800 737 732, text 0458 737 732 or visit 1800RESPECT.org.au for online chat and video call services. The number for Life Line is 13 11 14.See omnystudio.com/listener for privacy information.

Part 3 of 4. My guest for this week's episode is Quin Wills, CSO and Co-Founder of Ochre Bio, a pioneering biotechnology company developing RNA therapies for chronic liver diseases. Using a combination of genomic deep phenotyping, precision RNA medicine, and testing in live human donor livers, Ochre is developing therapies for liver health challenges ranging from increasing donor liver supply to reducing cirrhosis complications.

Denis DubouleCollège de FranceÉvolution du développement et des génomesAnnée 2023-2024Colloque - Barbara Treutlein : Understanding Human Brain Development with Organoids and Single-Cell AnalysisIntervenant(s)Barbara TreutleinETH, Zurich, Suisse

In this episode of the Epigenetics Podcast, we talked with Ana Cvejic from the Biotech Research & Innovation Centre at the University of Copenhagen about her work on using sc-multiomics to characterise human developmental hematopoiesis. The conversation starts by delving into Ana's research on hematopoiesis, starting with her work on identifying novel genes controlling blood traits in zebrafish models. She explains her transition to single-cell methodologies and the application of single-cell RNA sequencing to study hematopoietic cells in zebrafish, focusing on thrombocyte lineage commitment and gene expression. The discussion progresses to her groundbreaking study on human fetal hematopoiesis, where she combined single-cell RNA-seq with single-cell ATAC-seq to understand chromatin accessibility and gene expression dynamics. Ana then shares insights into the identification of new cell surface markers and the priming of hematopoietic stem cells, particularly in conditions like Down syndrome. Furthermore, she then elaborates on the construction of a phylogenetic tree of blood development using whole-genome sequencing of single-cell-derived hematopoietic colonies from healthy human fetuses. She explains the motivation behind this study, highlighting the insights gained regarding stem cell quantities, developmental timelines, and mutations in blood development. References Bielczyk-Maczyńska, E., Serbanovic-Canic, J., Ferreira, L., Soranzo, N., Stemple, D. L., Ouwehand, W. H., & Cvejic, A. (2014). A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis. PLoS genetics, 10(7), e1004450. https://doi.org/10.1371/journal.pgen.1004450 Athanasiadis, E. I., Botthof, J. G., Andres, H., Ferreira, L., Lio, P., & Cvejic, A. (2017). Single-cell RNA-sequencing uncovers transcriptional states and fate decisions in haematopoiesis. Nature communications, 8(1), 2045. https://doi.org/10.1038/s41467-017-02305-6 Ranzoni, A. M., Tangherloni, A., Berest, I., Riva, S. G., Myers, B., Strzelecka, P. M., Xu, J., Panada, E., Mohorianu, I., Zaugg, J. B., & Cvejic, A. (2021). Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis. Cell stem cell, 28(3), 472–487.e7. https://doi.org/10.1016/j.stem.2020.11.015   Related Episodes Single Cell Epigenomics in Neuronal Development (Tim Petros) ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells (Jason Buenrostro) Single-Cell Technologies using Microfluidics (Ben Hindson)   Contact Epigenetics Podcast on X Epigenetics Podcast on Instagram Epigenetics Podcast on Mastodon Epigenetics Podcast on Bluesky Epigenetics Podcast on Threads Active Motif on X Active Motif on LinkedIn Email: podcast@activemotif.com

These little critters are often seen on the beach, they are the worlds largest single cell organism and they can get up to the size of a softball!

The cell-surface proteome plays a critical role in immune-cell function; however, our ability to examine its interactions and spatial organization has previously been limited by available proteomic techniques. This episode explores the function of immune-cell membrane proteins and how the latest developments in spatial proteomics have enabled more detailed interrogation of these proteins and their spatial relationships.Our guest, Hanna van Ooijen, Immunology Application Scientist at Pixelgen Technologies guides us through the field, revealing a new technique that enables spatial analysis of the cell-surface proteome at a single-cell resolution and highlighting some exciting discoveries that it has facilitated.Contents:Introductions: 00:00-01:40Introducing Molecular Pixelation: 01:40-02:15Example applications of Molecular Pixelation: 02:15-03:20The role of membrane proteins in immune cell function: 03:20-07:25Traditional techniques to investigate cell membrane proteins: 07:15-10:20Recent improvements in investigative technology and our understanding of immunology: 10:20-11:10Challenges associated with current technologies: 11:10-13:50How Molecular Pixelation can address these challenges: 13:50-15:25Molecular Pixelation workflow: 15:25-17:55Tips for best practice when using molecular pixelation: 17:55-19:30Exciting discoveries using Molecular pixelations: 19:30-21:00Potential implications of molecular pixelation for the future of immunology: 21:00-24:00 Hosted on Acast. See acast.com/privacy for more information.

BUFFALO, NY- March 13, 2024 – A new #research paper was #published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 16, Issue 4, entitled, “Single-Cell RNA-seq reveals transcriptomic modulation of Alzheimer's disease by activated protein C.” Single-Cell RNA sequencing reveals changes in cell population in Alzheimer's disease (AD) model 5xFAD (5x Familial AD mutation) versus wild type (WT) mice. In this new study, researchers Mohammad Kasim Fatmi, Hao Wang, Lily Slotabec, Changhong Wen, Blaise Seale, Bi Zhao, and Ji Li from the University of South Florida, University of Mississippi Medical Center and the G.V. (Sonny) Montgomery VA Medical Center used single-cell RNA sequencing and bioinformatic analysis to analyze the effects of APC [Activated Protein C] treatment on AD transgenic mice. “In our investigation, we utilized transgenic mice that contain expression for five major amyloid pathologies that allow for rapid progression of AD and Aβ deposition known as 5xFAD mice.” The returned sequencing data was processed through the 10x Genomics CellRanger platform to perform alignment and form corresponding matrix to perform bioinformatic analysis. Alterations in glial cells occurred in 5xFAD versus WT, especially increases in microglia proliferation were profound in 5xFAD. Differential expression testing of glial cells in 5xFAD versus WT revealed gene regulation. Globally, the critical genes implicated in AD progression are upregulated such as Apoe, Ctsb, Trem2, and Tyrobp. Using this differential expression data, GO term enrichment was completed to observe possible biological processes impacted by AD progression. Utilizing anti-inflammatory and cyto-protective recombinant Activated Protein C (APC), the researchers uncovered inflammatory processes to be downregulated by APC treatment in addition to recuperation of nervous system processes. Moreover, animal studies demonstrated that administration of recombinant APC significantly attenuated Aβ burden and improved cognitive function of 5xFAD mice. “The downregulation of highly expressed AD biomarkers in 5xFAD could provide insight into the mechanisms by which APC administration benefits AD.” DOI - https://doi.org/10.18632/aging.205624 Corresponding authors - Bi Zhao - bizhao@usf.edu, and Ji Li - jli3@umc.edu Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205624 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, APC, Alzheimer's disease, inflammation About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at https://www.Aging-US.com​​ and connect with us: Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Spotify - https://open.spotify.com/show/1X4HQQgegjReaf6Mozn6Mc Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

Episode 11 - Innovations in Vaccine Research: Single Cell Immunity Development With Dr Fabian Otte Recorded Live from CROI 2024In this podcast episode, Dr Michael Saag converses with Dr Fabian Otte, a molecular biologist at the University of Basel in Switzerland, live from the 2024 Conference on Retroviruses and Opportunistic Infections (CROI). Dr Otte shares insights into the ongoing development of an innovative SARS-CoV-2 vaccine that employs a single-cell immunity development approach. Highlighting its potential benefits, such as broader and longer-lasting immunity compared with mRNA vaccines. This pioneering vaccine research might also extend to other RNA viruses in the future. 00:02 Introduction to the Podcast and Guest 00:28 Discussion on the Development of Next-Generation SARS-CoV-2 Vaccines 00:50 Exploring a Single-Cell Immunity Approach 01:10 Understanding the Vaccine Development Process 02:42 The Potential of the New Vaccine 03:13 Challenges and Advantages of the New Vaccine 05:07 The Impact of the Vaccine on Immune Response__________________________________________________Produced by IAS-USA, Going anti–Viral is a podcast for clinicians involved in research and care in HIV, its complications, and other viral infections. This podcast is intended as a technical source of information for specialists in this field, but anyone listening will enjoy learning more about the state of modern medicine around viral infections. Going anti-Viral's host is Dr Michael Saag, a physician, prominent HIV researcher at the University of Alabama at Birmingham, and volunteer IAS–USA board member. In most episodes, Dr Saag interviews an expert in infectious diseases or emerging pandemics about their area of specialty and current developments in the field. Other episodes are drawn from the IAS–USA vast catalogue of panel discussions, Dialogues, and other audio from various meetings and conferences. Email podcast@iasusa.org to send feedback, show suggestions, or questions to be answered on a later episode.Follow Going anti-Viral on: Apple Podcasts YouTube InstagramTikTok...

We are all the product of a reproductive process, yet reproductive biology, or the study of the processes and mechanisms involved in reproduction, is not well understood. Deepening our understanding of reproductive biology is crucial to advancing assistive reproductive technologies (ART) and advancing our collective comprehension of inheritance and evolution. Our guests for this episode are a couple, and we mean a literal married couple, of reproductive biology experts. Dr. Pavla Brachova and Dr. Nehemiah Alvarez, both working in the Eastern Virginia Medical School's Department of Physiological Sciences. In their collaborative work they aim to better understand and characterize the role of RNA and cellular events that impact ovarian function in women. We learn about their work with oocytes, which are single cells that grow and mature within the ovary and once fertilized provide the foundations of an embryo capable of maturing to a new individual. They outline how they use digital PCR (dPCR) and other methods to monitor RNA regulation in single cells and how progressing this work and lead to potential RNA-based therapies. In Cassie's career corner we hear childhood stories from each guest and learn about their respective career paths, which eventually collided and merged. They share insights on the importance of having mentors experienced in your field, the challenges of shared job searching, and the joys of collaborating as a couple with shared scientific interests.Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. 

Today, I am blessed to have here with me Caroline Alan. Dan Howard and Caroline Alan founded BEAM Minerals as a vehicle for promoting the amazing power of plant-based fulvic and humic minerals. Their personal experiences using these completely natural substances transformed their health and their understanding of healing. As a result of their experiences, Dan and Caroline are committed to providing completely pure, 100% bioavailable, sustainably produced, fulvic, and humic products. In this episode, Caroline Alan explains everything you need to know about minerals. Did you know that every single cell in your body requires minerals? Unfortunately, most people are mineral depleted. Caroline explains why you may be suffering from mineral deficiency and not getting enough minerals from the foods you eat. Luckily, Caroline reveals how you can replenish minerals through her products like Micro-BOOST, Electrolyze, and Insta-Lytes. Tune in as we chat about avoiding period symptoms with minerals, what you need to know about minerals on a carnivore diet, and why Caroline recommends liquid plant-based minerals.  Get Beam Mineral products here: www.beamminerals.com/ketokamp use the code ketokamp for 20% off your entire order. 

A $1.5 million project is underway to revert Rangiora High School's modern open learning space to single cell classrooms. Its giant classroom fitting 500 students opened in 2017 but was found difficult to work in, due to noise, visual distractions, and fluctuating temperatures. Principal Bruce Kearney says they're determined the open structure doesn't suit their vision for the school. "A lot of people talked about modern learning environments as being open barns- and they're not. What they're supposed to be is providing the ability for teachers, for students, classes, schools to be flexible." LISTEN ABOVESee omnystudio.com/listener for privacy information.

There are very few remaining locations on Earth that are untouched by humans, and those that do remain are in very extreme environments that are difficult to access.  However, accessing and studying life in these extreme environments can provide unique insights to the biology of life. Understanding how simple organisms adapt and survive in seemingly unlivable conditions is a unique field of study with the potential to inform and affect the human condition.  We're joined in this episode by Dr. Brandi Kiel Reese and Lydia Hayes-Guastella from the Dauphin Island Sea Lab at the University of South Alabama. They are both geomicrobiologists that study microbial life in extreme environments like the Mariana Trench and Antarctica.  They do an excellent job of painting a picture of how extreme conditions are in these environments and how they manage to collect and preserve samples from such harsh conditions. We learn about the various methods they use to analyze the microbial samples they collect, including the use of digital PCR (dPCR) to detect and quantify transcripts that would otherwise not be detectable given how few cells they're able to collect.   Brandi and Lydia also share their unpredictable career path journeys, while sharing some insights and learnings from their respective experiences. We learn what they each love about their work and what qualities is takes to be successful at what they do. Once again, we're reminded of what a small world it is, especially when you're in a specialty field such as geomicrobiology of extreme environments.   Visit the Absolute Gene-ius page to learn more about the guest, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. 

 Guillem Pratx from Stanford PhD clever single cell imaging for everyone!We chat to an A Prof Pratx from Stanford who's team has made possible single cell nuclear medicine imaging for under $100. nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,SNNMI,nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,PET,imaging,stanfordPlease let me know what you think about the video versions of the podcast.I am also looking for new material so please get in touch with me if you can contributewith an interview.Direct link to iTuneshttps://itunes.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicinie-podcast/id1444565219?mt=2Older podcastshttps://podcasts.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicine-podcast/id94286547You can get the podcast page at both http://nuccast.com and http://www.nuccast.com with the feed to put into iTunes or juice or your favourite podcast software can be found at http://molcast.com/.The cardiac subset of the podcast can be found at http://cardiac.nuccast.com/Please pass on information about this podcast to your colleagues and to your CPD provider.Link to Video Link to Video fileLink to Audio file Link to Audio fileOr you can subscribe by entering your email address below and you will be informed of new episodesEnter your email address:Delivered by FeedBurnerMost importantly of all please help this podcast by contributing your opinions, Sound files, and emailsnucmedpodcast@gmail.comAll contributions welcome, especially as sound files to nucmedpodcast@gmail.com.@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-GB; mso-fareast-language:EN-US;}div.WordSection1 {page:WordSection1;}

 Guillem Pratx from Stanford PhD clever single cell imaging for everyoneWe chat to an A Prof Pratx from Stanford who's team has made possible single cell nuclear medicine imaging for under $100. nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,SNNMI,nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,PET,imaging,stanfordPlease let me know what you think about the video versions of the podcast.I am also looking for new material so please get in touch with me if you can contributewith an interview.Direct link to iTuneshttps://itunes.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicinie-podcast/id1444565219?mt=2Older podcastshttps://podcasts.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicine-podcast/id94286547You can get the podcast page at both http://nuccast.com and http://www.nuccast.com with the feed to put into iTunes or juice or your favourite podcast software can be found at http://molcast.com/.The cardiac subset of the podcast can be found at http://cardiac.nuccast.com/Please pass on information about this podcast to your colleagues and to your CPD provider.Link to Video Link to Video fileLink to Audio file Link to Audio fileOr you can subscribe by entering your email address below and you will be informed of new episodesEnter your email address:Delivered by FeedBurnerMost importantly of all please help this podcast by contributing your opinions, Sound files, and emailsnucmedpodcast@gmail.comAll contributions welcome, especially as sound files to nucmedpodcast@gmail.com.@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-GB; mso-fareast-language:EN-US;}div.WordSection1 {page:WordSection1;}

 Guillem Pratx from Stanford PhD clever single cell imaging for everyoneWe chat to an A Prof Pratx from Stanford who's team has made possible single cell nuclear medicine imaging for under $100. nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,SNNMI,nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,PET,imaging,stanfordPlease let me know what you think about the video versions of the podcast.I am also looking for new material so please get in touch with me if you can contributewith an interview.Direct link to iTuneshttps://itunes.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicinie-podcast/id1444565219?mt=2Older podcastshttps://podcasts.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicine-podcast/id94286547You can get the podcast page at both http://nuccast.com and http://www.nuccast.com with the feed to put into iTunes or juice or your favourite podcast software can be found at http://molcast.com/.The cardiac subset of the podcast can be found at http://cardiac.nuccast.com/Please pass on information about this podcast to your colleagues and to your CPD provider.Link to Video Link to Video fileLink to Audio file Link to Audio fileOr you can subscribe by entering your email address below and you will be informed of new episodesEnter your email address:Delivered by FeedBurnerMost importantly of all please help this podcast by contributing your opinions, Sound files, and emailsnucmedpodcast@gmail.comAll contributions welcome, especially as sound files to nucmedpodcast@gmail.com.@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-GB; mso-fareast-language:EN-US;}div.WordSection1 {page:WordSection1;}

 Guillem Pratx from Stanford PhD clever single cell imaging for everyone!We chat to an A Prof Pratx from Stanford who's team has made possible single cell nuclear medicine imaging for under $100. nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,SNNMI,nuccast.com,NIF,UOM,nuclearmedicine,theranostics,cancer,radioactive,mbciu,PET,imaging,stanfordPlease let me know what you think about the video versions of the podcast.I am also looking for new material so please get in touch with me if you can contributewith an interview.Direct link to iTuneshttps://itunes.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicinie-podcast/id1444565219?mt=2Older podcastshttps://podcasts.apple.com/au/podcast/the-nuclear-medicine-and-molecular-medicine-podcast/id94286547You can get the podcast page at both http://nuccast.com and http://www.nuccast.com with the feed to put into iTunes or juice or your favourite podcast software can be found at http://molcast.com/.The cardiac subset of the podcast can be found at http://cardiac.nuccast.com/Please pass on information about this podcast to your colleagues and to your CPD provider.Link to Video Link to Video fileLink to Audio file Link to Audio fileOr you can subscribe by entering your email address below and you will be informed of new episodesEnter your email address:Delivered by FeedBurnerMost importantly of all please help this podcast by contributing your opinions, Sound files, and emailsnucmedpodcast@gmail.comAll contributions welcome, especially as sound files to nucmedpodcast@gmail.com.@font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman";}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-GB; mso-fareast-language:EN-US;}div.WordSection1 {page:WordSection1;}

References Nat Protoc. 2017 Nov;12(11):2342-2354 Annual Review of Immunology 2021. Volume 39. pp 279-311 Immunity 2022. Volume 55, Issue 8. Pages 1402-1413.e4 --- Send in a voice message: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/message

This week's EYE ON NPI is going to fuel up your portable battery-powered designs with the Texas Instruments BQ27427 System-Side Impedance Track™ Single-Cell Battery Fuel Gauge (https://www.digikey.com/en/product-highlight/t/texas-instruments/bq27427-single-cell-battery-fuel-gauge) a powerful and inexpensive way to add high quality battery monitoring for charge-rate and aging tracking at the system side. Lithium Ion / Polymer batteries (https://www.digikey.com/en/products/filter/batteries-rechargeable-secondary/91) - you love them for their low cost, high power density, and 3.7V nominal voltage, but keeping them recharged and letting people know exactly how much battery life is left is non-trivial. Like most batteries, common LiPoly/LiIon's start with a high peak voltage right after charging of about 4.2V, drop quickly to about 3.7V and then slowly drift down till they get to 3.0V at which point the cut-off circuitry disconnects until they are recharged. It's really easy to tell when the battery is 90% or greater charge, just look for a voltage of 3.9V or higher. It's also pretty easy to tell when they're 10% or less, the voltage will be 3.3V or lower. But that in-between section is tough because the slope during discharge is very shallow and the voltage changes quite a bit with aging, temperature, and discharge rate (https://www.ti.com/lit/an/snva533/snva533.pdf) So while there are battery monitors that can use just the voltage across the battery terminals, they're not going to be as precise as a coulomb-counter design that takes into account both voltage and current. From TI's White Paper on the topic (https://www.ti.com/lit/wp/slpy002/slpy002.pdf): "An impedance-based battery fuel gauge, as the name implies, uses the measured impedance of the battery's cells as a key input to its remaining capacity predictions. The gauge measures and stores in real-time the battery pack's resistance as a function of state-of-charge. The real-time resistance profiles along with the stored battery open-circuit voltage tables (open-circuit voltage vs. state-of-charge) enables the gauge to predict the battery pack's discharge curve (by adjusting for the IR drop) under any system-use condition and temperature. The algorithm uses current integration (coulomb counting) when the system is ON, and open-circuit voltage measurement when the system is OFF or in SLEEP to adjust remaining state-of-charge (RSOC) up or down (for charge or discharge) the predicted discharge curve. By using the predicated discharge curve, the gauge can accurately calculate the battery pack's remaining discharge capacity (RM) and the system's run-time to empty (RTTE). Since an impedance track gauge continuously adjusts RM and FCC for impedance and the change in impedance, rate and temperature inefficiencies and aging are inherently taken into account, enabling the gauge to maintain a high level of accuracy throughout the life of the system." Texas Instruments has a few chips in the Impedance Track family (https://www.ti.com/video/6287050244001) - the BQ27427 charger (https://www.digikey.com/en/products/detail/texas-instruments/BQ27427YZFR/17748369) is the latest to come out. It's very small, coming as a 9-pin 1.6x1.6 BGA with the center pad being a shared ground so you don't need plugged vias to use. It's also really easy to use, requiring only 2 small stabilization capacitors. Internal temperature monitoring means you don't need a separate thermistor. The high-side 7 mΩ current sense resistor also means one less part on the BOM. I2C pins are used to communicate with the monitor and set battery characteristics. There's also an optional input that can be used to connect to a mechanical or electrical battery insertion switch or to an external thermistor, and an optional output that can be used as a battery low indicator. Because so much is integrated, and the chip is so small, its very easy to pop it into your design between the battery terminal and the VBAT lines that go to your battery charger and power supply. The peak battery voltage and capacity are easy to look up since they're written on the battery. For other configuration settings you can use BQStudio / GaugeStudio (https://www.ti.com/tool/BQSTUDIO) with the TI battery devboard to characterize and customize the learning cycle. Either way, you will need to write the configuration to the sensor on every boot. Pick up the Texas Instruments BQ27427 (https://www.digikey.com/short/18pb1ftw) and other chips from the Impedance Track family (https://www.digikey.com/en/products/result?s=N4IgjCBcoLQCxVAYygMwIYBsDOBTANCAG4B2aWehA9lANrhwCsAnBALqEAOALlCCAF8hQA0) stocked at DigiKey for immediate shipment. Order today and you'll get precision lipoly monitoring that will work with any setup or battery configuration by tomorrow afternoon!

Dr Philip Smith, Digital and Education Editor of Gut and Honorary Consultant Gastroenterologist at the Royal Liverpool Hospital, Liverpool, UK interviews Professor Kathy DelGiorno, from the Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA, on the paper 'It is better to light a candle than to curse the darkness: single-cell transcriptomics sheds new light on pancreas biology and disease' published in paper copy in Gut in June 2023 and available online: https://gut.bmj.com/content/72/6/1211 Please subscribe to the Gut Podcast via all podcast platforms, including Apple Podcasts, Google Podcasts, Stitcher and Spotify, to get the latest podcast every month. If you enjoy our podcast, please consider leaving us a review or a comment on the Gut Podcast iTunes page (https://podcasts.apple.com/gb/podcast/gut-podcast/id330976727).

Dr Philip Smith, Digital and Education Editor of Gut and Honorary Consultant Gastroenterologist at the Royal Liverpool Hospital, Liverpool, UK interviews Professor Kathy DelGiorno, from the Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee, USA, on the paper 'It is better to light a candle than to curse the darkness: single-cell transcriptomics sheds new light on pancreas biology and disease' published in paper copy in Gut in June 2023 and available online: https://gut.bmj.com/content/72/6/1211 Please subscribe to the Gut Podcast via all podcast platforms, including Apple Podcasts, Google Podcasts, Stitcher and Spotify, to get the latest podcast every month. If you enjoy our podcast, please consider leaving us a review or a comment on the Gut Podcast iTunes page (https://podcasts.apple.com/gb/podcast/gut-podcast/id330976727).

A new research paper was published in Aging (Aging-US) Volume 15, Issue 8, entitled, “Single-cell transcriptomic analysis uncovers diverse and dynamic senescent cell populations.” Senescence is a state of enduring growth arrest triggered by sublethal cell damage. Given that senescent cells actively secrete proinflammatory and matrix-remodeling proteins, their accumulation in tissues of older persons has been linked to many diseases of aging. Despite intense interest in identifying robust markers of senescence, the highly heterogeneous and dynamic nature of the senescent phenotype has made this task difficult. In this new study, researchers Noah Wechter, Martina Rossi, Carlos Anerillas, Dimitrios Tsitsipatis, Yulan Piao, Jinshui Fan, Jennifer L. Martindale, Supriyo De, Krystyna Mazan-Mamczarz, and Myriam Gorospe from the National Institute on Aging set out to comprehensively analyze the senescent transcriptome of human diploid fibroblasts at the individual-cell scale by performing single-cell RNA-sequencing analysis through two approaches. “Here, we used single-cell RNA sequencing (scRNA-seq) analysis to document both the diverse transcriptomes of human senescent fibroblasts at an individual-cell scale, and the changes in the transcriptome over time during etoposide-triggered senescence.” First, the researchers characterized the different cell states in cultures undergoing senescence triggered by different stresses, and found distinct cell subpopulations that expressed mRNAs encoding proteins with roles in growth arrest, survival and the secretory phenotype. Second, they characterized the dynamic changes in the transcriptomes of cells as they developed etoposide-induced senescence; by tracking cell transitions across this process, the researchers found two different senescence programs that developed divergently, one in which cells expressed traditional senescence markers such as p16 (CDKN2A) mRNA, and another in which cells expressed long noncoding RNAs and splicing was dysregulated. Finally, they obtained evidence that the proliferation status at the time of senescence initiation affected the path of senescence, as determined based on the expressed RNAs. “We propose that a deeper understanding of the transcriptomes during the progression of different senescent cell phenotypes will help develop more effective interventions directed at this detrimental cell population.” DOI - https://doi.org/10.18632/aging.204666 Corresponding authors - Krystyna Mazan-Mamczarz - krystyna.mazan-mamczarz@nih.gov, and Myriam Gorospe - myriam-gorospe@nih.gov Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.204666 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, senescence, single-cell analysis, transcriptome About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at https://www.Aging-US.com​​ and connect with us: SoundCloud - https://soundcloud.com/Aging-Us Facebook - https://www.facebook.com/AgingUS/ Twitter - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

In this special episode, we're joined by Cytosurge CSO Tobias Beyer, Ph.D., and SEED Biosciences CEO and Co-Founder Georges Muller, Ph.D., for an overview of gene editing with Cytosurge's FluidFM® in combination with DispenCell™ dispensing technologies.Tobias and Georges explain the FluidFM® technique and how it differs from traditional CRISPR methods along with the advantages the technology has over other methods of gene editing.For a transcript of this episode, please visit this episode's page on Buzzsprout.Our Sponsor for this EpisodeMolecular Devices makes scientific breakthroughs possible for academia, biopharma and government customers. Dedicated to enabling life science labs of the future, where innovative technology and novel research meet, Molecular Devices empowers scientists to advance discovery, driving earlier diagnoses and safer therapeutics for patients. Spanning cell line development, 3D biology and drug screening, their automated, end-to-end solutions streamline and scale complex workflows while integrated machine learning-enabled analytics allow researchers to mine data easily for insights. Molecular Devices is the innovation partner that empowers scientists with next-generation technology to advance discoveries, improving the quality of life everywhere. Learn more about Molecular Devices by visiting: https://www.moleculardevices.com/ Stay connected with SLASOnline at www.slas.orgFacebookTwitter @SLAS_OrgLinkedInInstagram @slas_orgYouTubeAbout SLASSLAS (Society for Laboratory Automation and Screening) is an international professional society of academic, industry and government life sciences researchers and the developers and providers of laboratory automation technology. The SLAS mission is to bring together researchers in academia, industry and government to advance life sciences discovery and technology via education, knowledge exchange and global community building.  For more information about SLAS, visit www.slas.org.Upcoming SLAS Events: SLAS 2023 Microscales Innovation in Life Sciences Symposium September 14-15, 2023 La Jolla, CA, USA SLAS 2023 Sample Management Symposium October 12-13, 2023 Washington, D.C., USA SLAS 2023 Data Sciences and AI Symposium November 14-15, 2023 Basel, Switzerland SLAS2024 International Conference and Exhibition February 3-7, 2024 Boston, MA, USA

Single-cell metabolomics is focused on detecting, understanding, and interpreting metabolites and lipids at single-cell resolution. Theodore Alexandrov and Anne Le discuss recent technical advances in the field with Chief Editor Barbara Cheifet. Hosted on Acast. See acast.com/privacy for more information.

Are you a nail tech? If you want help building and growing your business, skills and confidence we can help you right now over on www.clawgasmic.comToday we speak to the fantastic Yan from @yan.thipham her work is unbelievable, and her attention to detail and designs are out of this world. I loved getting to know the amazing human behind the profile, make sure you give her a follow @yan.thipham If you want to check out the new merch from @nailorderclick here www.nailorder.co.ukInstagram @nailorderTik Tok @nailorderTo contact Chan for ad enquiries chan@clawgasmic.comfollow us on Instagram https://www.instagram.com/clawgasmic/subscribe to our YouTube https://www.youtube.com/c/clawgasmicJoin our family www.clawgasmic.com

It's been a long road to mastering the cell, but biological scientists think they are getting closer and closer to understanding the fundamental mechanics of the kernels of life that make up our bodies. Decades after the sequencing of the first human genome, we now have a much more comprehensive understanding of how to discover a cell's functions — and increasingly, the tools to actually analyze and prove that our models and theories about them are correct. That's been the domain of single-cell analysis and a novel technique in genetic science, which has been dubbed “perturbation biology”: making extremely small changes to the genetic code inside of cells and then observing how that cell's functions change. What began with 18 cells and limited observational data in a single lab has now grown exponentially to hundreds of thousands of cells and millions of observations globally. That massive increase in data has forced the creation of a whole new set of analytical tools to process this data and derive foundational insights into the workings of cells. How do all of these new laboratory experiments work and what kind of software tools are needed to progress the most advanced theories today? Joining host Danny Crichton on “Securities” this episode is Rahul Satija, an associate professor at New York University and a core member of the New York Genome Center as well as Lux's own Shaq Vayda. We'll talk about how biological tools like CRISPR power perturbation bio, why scientists are increasingly moving away from indirect experiments to direct experiments and what that means for the future of the field, how we comprehend cell heterogeneity, if we're getting closer to “fundamental truth” in biology, and finally, why theoretical molecular scientists are increasingly going to need large-scale clinical trials for the next-generation of health treatments.

In this week's episode we'll discuss how azacytidine therapy influences the contributions of mutated HSC clones to hematopoiesis in MDS and CMML, learn more about the risk of venous thromboembolism in patients with adult-type diffuse glioma, and discuss the importance of 1p32 deletions as an independent and adverse prognostic factor in myeloma.

Caroline Alan is a health survivor and mineral enthusiast. Coming from a career in the corporate world, Caroline found herself struggling physically, mentally and energetically.  During her journey back to health, Caroline encountered plant-based minerals and their incredible ability to support a return to natural balance in the body.  As a result, she has become devoted to educating people about the benefits of mineral replenishment and the specific efficacy of plant-based humic and fulvic substances.   Caroline's research into why minerals are important in human physiology and how they work in the body, has taken her deep into microbiology, molecular biology, cellular biology, agricultural soil science and the study of plant decomposition. In the process, Caroline has found ways to help others understand the effects of mineral depletion and why plant-based humic and fulvic mineral supplementation support healing and optimal health.   Caroline is the Co-Founder & CEO of BEAM Minerals, which provides liquid humic and fulvic mineral supplements. “These humic and fulvic complexes, they're like Mother Nature's answer to mineral supplementation,” Caroline says.

This month on JHLT: The Podcast, the JHLT Digital Media Editors review two studies from the February issue of The Journal of Heart and Lung Transplantation—and bring in a couple of experts to help them make sense of some new technology.   First, the editors explore a pre-clinical study entitled “The dynamic cellular landscape of grafts with acute rejection after heart transplantation,” which comes from Kong and colleagues at Zhejiang University in Hangzhou, China.   Single cell technologies are emerging as non-biased techniques to discover novel biological pathways in a variety of pre-clinical models and in human tissue both in health and disease. To help the editors—and you!—understand single cell approaches and this study, JHLT editors Ben Kopecky, MD, PhD and Kory Lavine, MD, PhD, from the Washington University St. Louis, appear in the episode to explore the methodology and what the study tells us.   Second, the editors welcome author Sam Rayner, MD from the University of Washington in Seattle, USA, to discuss the paper by Hirsch and colleagues, “Circulating markers of inflammation and angiogenesis and clinical outcomes across subtypes of pulmonary arterial hypertension.”   When considering differences in pathophysiology of the subtypes of PAH, changes in biomarkers in angiogenesis and inflammation may provide useful insights and potential therapeutic targets. The authors of this study prospectively looked at 33 biomarkers of angiogenesis and inflammation in a cohort of patients across various PAH etiologies, and made correlations to clinical outcomes.   Follow along at www.jhltonline.org/current, or, if you're an ISHLT member, log in at ishlt.org/journal-of-heart-lung-transplantation.  Don't already get the Journal and want to read along? Join the International Society of Heart and Lung Transplantation at www.ishlt.org for a free subscription, or subscribe today at www.jhltonline.org.    

Severe period pain, infertility and even depression - living with endometriosis can have serious effects on quality of life. Will new technologies finally yield therapy for those with the condition? Joining Jonathan to discuss this is Dr Kate Lawrenson, associate professor in the Department of Obstetrics and Gynecology at Cedars-Sinai Hospital in LA.

In this bonus episode Associate Editor, Dr. Bertie Göttgens and Dr. Ravi Majeti discuss the series on Single Cell Genomics and Heme Malignancies-Leukemia/MPNs.

A new research paper was published on the cover of Aging (listed as "Aging (Albany NY)" by Medline/PubMed and "Aging-US" by Web of Science) Volume 15, Issue 1, entitled, “Single-cell transcriptomics of peripheral blood in the aging mouse.” Compositional and transcriptional changes in the hematopoietic system have been used as biomarkers of immunosenescence and aging. In this new study, researchers Yee Voan Teo, Samuel J. Hinthorn, Ashley E. Webb, and Nicola Neretti from Brown University used single-cell RNA-sequencing to study the aging peripheral blood in mice and characterize the changes in cell-type composition and transcriptional profiles associated with age. “Here, we applied scRNA-seq on young and old mice to dissect the transcriptional and cell composition changes of all cell types in the peripheral blood with age.” The team identified 17 clusters from a total of 14,588 single cells. They detected a general upregulation of antigen processing and presentation and chemokine signaling pathways and a downregulation of genes involved in ribosome pathways with age. In old peripheral blood, the researchers also observed an increased percentage of cells expressing senescence markers (Cdkn1a, and Cdkn2a). In addition, a cluster of activated T cells exclusively found in old blood was detected, with lower expression of Cd28 and higher expression of Bcl2 and Cdkn2a, suggesting that the cells are senescent and resistant to apoptosis. “Finally, targeting senescent cells using genetic approaches has been shown to ameliorate the aging phenotype [34, 35]. More recently, senolytics drugs are being identified or developed to target apoptotic pathways because senescent cells are known to be apoptosis-resistant [34]. Therefore, the Bcl2+ old T cells that we identified in old mice can potentially be targeted pharmacologically to ameliorate the phenotypes associated with the aging of the immune system.” DOI: https://doi.org/10.18632/aging.204471 Corresponding Author: Nicola Neretti - nicola_neretti@brown.edu Keywords: aging, single-cell transcriptomics, senescence, peripheral blood Sign up for free Altmetric alerts about this article: https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.204471 About Aging-US: Launched in 2009, Aging (Aging-US) publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at www.Aging-US.com​​ and connect with us: SoundCloud – https://soundcloud.com/Aging-Us Facebook – https://www.facebook.com/AgingUS/ Twitter – https://twitter.com/AgingJrnl Instagram – https://www.instagram.com/agingjrnl/ YouTube – https://www.youtube.com/agingus​ LinkedIn – https://www.linkedin.com/company/aging/ Reddit – https://www.reddit.com/user/AgingUS Pinterest – https://www.pinterest.com/AgingUS/ For media inquiries, please contact media@impactjournals.com.

Sara Safari is an author, speaker, mountain climber, college professor, electrical engineer, and advocate for women's empowerment. She received the award for The Global Citizen from United Nations Association in 2015 and serves as a board member and development director at Empower Nepali Girls foundation. Sara received the Outstanding Practice with Broad Impact award from the International Leadership Association in 2017. Safari is the founder of Climb Your Everest, a non-profit - and organization dedicated to providing "educational programs that empower marginalized women through collaborative learning of leadership skills."Sara is the first Iranian to climb the Seven Summits, the seven highest peaks in each continent. She is climbing to raise funds for seven organizations that are empowering women. She published her books “Follow my Footsteps,” “Above the Mountain Shadow,” and “Making a Difference” to share her story with the world. She is working on a featured film and a documentary with a talented team of directors and producers with extensive experience working on complex social justice issues.Connecting with SaraLinkedInResourcesDocumentary - Aftershock on NetflixTed Talk - Climb Your EverestA Quote From This Episode"It felt like my whole life was summarized into this one second. And these were the most important seconds of my life. Every single cell in my body was dancing. I felt like I was one with the whole world."About The International Leadership Association (ILA)The ILA was created in 1999 to bring together professionals interested in the study, practice, and teaching of leadership. My Approach to HostingThe views of my guests do not constitute "truth." Nor do they reflect my personal views in some instances. However, they are important views to be aware of. Nothing can replace your own research and exploration.Connect with Scott AllenWebsite

Tariq Kassum, CEO of Cambridge, Mass.-based Celsius Therapeutics, on using single cell technology for drug discovery.

In this episode of the Epigenetics Podcast, we caught up with Tim Petros from the Eunice Kennedy Shriver National Institute of Child Health and Human Development at the NIH to talk about his work on Single Cell Epigenomics in Neuronal Development.  The Petros lab focuses on “interneurons”, their diversity and how environmental signals interact to generate this diversity. This subgroup of neurons comprise about 20% of neutrons in the brain, however, they are the primary source of inhibition. Furthermore, interneurons are critical components in modulating information flow throughout the nervous system. The Petros lab seeks to uncover the genetic programs that lead to the incredible diversity in interneurons, as well as how the local environment influences this process.  To lay a foundation for this and to provide a data-base for other researchers the Petros lab generated an epigenome atlas of neural progenitor cells of the mouse brain. This data includes scRNA-Seq, snATAC-Seq, CUT&Tag (H3K4me3, H3K27me3), CUT&RUN (H3K27ac), Hi-C and Capture-C. This data can be downloaded at the link below:   https://www.nichd.nih.gov/research/atNICHD/Investigators/petros/data-sharing    References Datasets: https://www.nichd.nih.gov/research/atNICHD/Investigators/petros/data-sharing Quattrocolo G, Fishell G, Petros TJ. Heterotopic Transplantations Reveal Environmental Influences on Interneuron Diversity and Maturation. Cell Rep. 2017 Oct 17;21(3):721-731. doi: 10.1016/j.celrep.2017.09.075. PMID: 29045839; PMCID: PMC5662128. Dongjin R Lee, Christopher Rhodes, Apratim Mitra, Yajun Zhang, Dragan Maric, Ryan K Dale, Timothy J Petros (2022) Transcriptional heterogeneity of ventricular zone cells in the ganglionic eminences of the mouse forebrain eLife 11:e71864 https://doi.org/10.7554/eLife.71864 Rhodes, C. T., Thompson, J. J., Mitra, A., Asokumar, D., Lee, D. R., Lee, D. J., Zhang, Y., Jason, E., Dale, R. K., Rocha, P. P., & Petros, T. J. (2022). An epigenome atlas of neural progenitors within the embryonic mouse forebrain. Nature communications, 13(1), 4196. https://doi.org/10.1038/s41467-022-31793-4   Related Episodes The Role of Histone Dopaminylation and Serotinylation in Neuronal Plasticity (Ian Maze) Single-Cell Technologies using Microfluidics (Ben Hindson, CSO of 10x Genomics) The Role of DNA Methylation in Epilepsy (Katja Kobow)   Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com

Dr. George Emanuel shares about single-cell spacial genomics in this podcast episode. Dr. Emanuel is the Scientific Cofounder and Director of Technology and Partnerships at Vizgen, a biotechnology company that is dedicated to pioneering the next generation of spatially resolved transcriptomics profiling tools to provide new insight into the biological systems that underlie human health and disease. Spatial genomics is a new frontier in molecular biology, which aims to assay the genomic information of single cells within their native tissue environment, combining spatial transcriptomics with single-cell sequencing. This exciting technology was named Nature's "Method of the Year 2020"!Dr. Emanuel has spent the past decade developing high-throughput spatial profiling technologies including extending Vizgen's MERFISH (Multiplexed Error-Robust Fluorescence in situ Hybridization) technology to profile 10,000 genes in the same sample. He received dual undergraduate degrees from the University of Colorado, and a Ph.D. in Biophysics from Harvard University.On This Episode We Discuss:Single-cell spatial genomicsDifference between sequencing genes and analyzing spatial gene expressionInsight for researchers through gene expression analysisMERFISH & MERSCOPELimitations of the testingAreas of research that MERFISH can be applied toHow can researchers get access to a MERSCOPE PlatformIf you're interested in learning more about Vizgen's technology, visit their website to play around with the MERSCOPE Vizualizer, and check out their papers on mapping the brain and how MERFISH was demonstrated on 10,000 genes.Keep up to date with the latest developments at Vizgen by following them on Twitter, Facebook, and LinkedIn, and connect with our guest, Dr. George Emanuel on Linkedin!Stay tuned for the next new episode of DNA Today on April 8, 2022 where our host, Kira Dineen, will be on the other side of the mic. She will be interviewed by James Fodor of The Science of Everything Podcast about genetic testing. New episodes are released on Fridays. In the meantime, you can binge over 175 other episodes on Apple Podcasts, Spotify, streaming on the website, or any other podcast player by searching, “DNA Today”. Episodes since 2021 are also recorded with video which you can watch on our YouTube channel. DNA Today is hosted and produced by Kira Dineen. Our social media lead is Corinne Merlino. Our video lead is Amanda Andreoli. See what else we are up to on Twitter, Instagram, Facebook, YouTube and our website, DNApodcast.com. Questions/inquiries can be sent to info@DNApodcast.com. PerkinElmer Genomics is a global leader in genetic testing focusing on rare diseases, inherited disorders, newborn screening, and hereditary cancer. Testing services support the full continuum of care from preconception and prenatal to neonatal, pediatric, and adult. Testing options include sequencing for targeted genes, multiple genes, the whole exome or genome, and copy number variations. Using a simple saliva or blood sample, PerkinElmer Genomics answers complex genetic questions that can proactively inform patient care and end the diagnostic odyssey for families. Learn more at PerkinElmerGenomics.com. (SPONSORED)Polygenic risk scores (or PRS) are a powerful way to identify an individual's risk of common disease by analyzing up to millions of genetic variants spread across the genome. Allelica has launched the first PRS test on the market to calculate ancestry-specific PRS for breast cancer, prostate cancer, coronary artery diseases, Type 2 Diabetes and Alzheimer's disease and deliver the results in a clinical-grade report. Order your test today at order.allelica.com. For 25% off AND free shipping, use code “DNATODAY” at checkout. Allelica: Empowering the next generation of clinical genomics. Check out Allelica' interview about PRS with co-Founder and CEO Giordano Bottà on Episode #168 of “DNA Today”! (SPONSORED)Sema4 offers an extensive portfolio of health information solutions, including pre-clinical and clinical trial support, analytics for actionable insights, and advanced sequencing services. Sema4's family of precision oncology products and services, Sema4 Signal®, provides data-driven care solutions with advanced analytics, digital tools, and exome-based somatic and hereditary cancer genomic tests. Learn more about their mission at Sema4.com. Sema4, a patient-centered health intelligence company. (SPONSORED)

In this episode of the Epigenetics Podcast, we caught up with Ben Hindson, Chief Scientific Officer at 10X Genomics, to talk about single-cell technologies using microfluidics. Epigenetics has moved well past a simple understanding of a single epigenetic layer of control at genomic regions of interest, thanks to advances in many techniques and the application of “multiomics”. We can now analyze genome-wide histone modification patterns, transcription factor binding profiles, chromatin accessibility profiles, three-dimensional chromosomal conformation, and DNA methylation dynamics combined with transcriptomic analyses and associated analytical platforms. Bulk Assays, like ATAC-Seq or ChIP, despite all their advantages, do not provide information about the chromatin states of individual subpopulations of cells within a sample. To identify chromatin features in heterogeneous populations, such as blood, pancreas, and brain, those analysis need to be performed at a single-cell level. 10X Genomics has been at the forefront of the movement into the single cell space and in this Episode we discuss this work with Ben Hindson, CSO of 10X genomics.   References https://www.10xgenomics.com/products/single-cell-multiome-atac-plus-gene-expression Spectrum of Innovation Multiomic Epigenetic Analysis Turns Short Stories into Epic Tales   Related Episodes Epigenome-based Precision Medicine (Eleni Tomazou) ATAC-Seq, scATAC-Seq and Chromatin Dynamics in Single-Cells Investigating the Dynamics of Epigenetic Plasticity in Cancer with Single Cell Technologies (Céline Vallot)   Contact Active Motif on Twitter Epigenetics Podcast on Twitter Active Motif on LinkedIn Active Motif on Facebook Email: podcast@activemotif.com

In this week's episode of the Genetics Unzipped podcast, we're exploring groundbreaking discoveries about the secret sex lives of cancer cells, and what it means for our understanding of tumour growth, evolution and treatment. Dr Kat Arney tells the story of how we discovered cancer cells were having sex, plus we look into why female tumours and male tumours act so differently.Full show notes, transcript, music credits and references online at GeneticsUnzipped.com Follow us on Twitter @GeneticsUnzipThis episode of Genetics Unzipped was written and presented by Kat Arney with audio production by Sally Le Page.This podcast is produced by First Create the Media for the Genetics Society - one of the oldest learned societies in the world dedicated to supporting and promoting the research, teaching and application of genetics.

It's like measuring our sleep with 800 pizzas on ad-free Twitter, and the end of bread is a cookie pizza with spicy McPlants.