Medizin - Open Access LMU - Teil 06/22

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8556/1/8556.pdf Scriba, Peter Christian; Müller-Esch, G.; Kähler, U.; Peters, A.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8557/1/8557.pdf Scriba, Peter Christian; Drenkhan, M.; Matthaei, S.; Klein, H. H.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8559/1/8559.pdf Scriba, Peter Christian

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8560/1/8560.pdf Scriba, Peter Christian; Wenzel, K. W.; Heesemann, Jürgen; Wenzel, B. E.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8561/1/8561.pdf Scriba, Peter Christian; Otte, M.; Stöcker, W. ddc:610,

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8566/1/8566.pdf Poschmann, A.; Schwarting, K.; Scriba, Peter Christian; Otte, M.; Stöcker, W.; Horbach, E.

Schlechte Scanvorlage.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8569/1/8569.pdf Kling-Steines, B.; Scriba, Peter Christian; Hötzel, D. ddc:6

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8571/1/8571.pdf Scriba, Peter Christian; Grammerstorf, S.; Heesemann, Jürgen; Wenzel, B. E.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8573/1/8573.pdf Scriba, Peter Christian; Müller-Esch, G.; Berkel, H.; Stendel, M.; Kentsch, M.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8595/1/8595.pdf Scriba, Peter Christian; Lawrenz, R.; Gerzer, R.; Alt, J.; Klingler, W.; Müller-Esch, G.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8596/1/8596.pdf Scriba, Peter Christian; Alt, J.; Ball, P.; Gerzer, R.; Müller-Esch, G.; Potratz, J.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8597/1/8597.pdf Scriba, Peter Christian; Alt, J.; Klohs, S.; Ball, P.; Heim, J. M.; Potratz, J.; Müller-Esch, G. d

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8598/1/8598.pdf Scriba, Peter Christian; Lawrenz, R.; Ball, P.; Heim, J. M.; Potratz, J.; Müller-Esch, G. ddc:610, M

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8599/1/8599.pdf Scriba, Peter Christian; Otte, M.; Stöcker, W. ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8600/1/Scriba-Peter_C._8600.pdf Scriba, Peter Christian; Matthaei, S.; Klein, H. H.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8667/1/8667.pdf Conzelmann, Karl-Klaus

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8675/1/nerve_growth_factor_8675.pdf Rohrer, H.; Meyer, Michael; Lindholm, D.; Heumann, R.; Bandtlow, C. E.; Thoenen, Hans ddc:610, Medizin 0

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8928/1/8928.pdf Scriba, Peter Christian; Grammerstorf, S.; Heesemann, Jürgen; Wenzel, B. E.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8930/1/8930.pdf Scriba, Peter Christian ddc:610, Medizin 0

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8932/1/8932.pdf Scriba, Peter Christian; Matthaei, S.; Klein, H. H.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8936/1/8936.pdf Scriba, Peter Christian ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8937/1/8937.pdf Scriba, Peter Christian; Otte, M.; Stöcker, W. ddc:610, M

Kein Inhaltsverzeichnis, keine Seitenzahl.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8983/1/8983.pdf Kellerer, Albrecht M.; Roos, H.; Fitzek, M.; Thomas, W.-H. ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9033/1/9033.pdf Kellerer, Albrecht M.; Fromke, E.; Fenn, S.; Trutschler, K.; Ponsel, G.; Hieber, L.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9034/1/9034.pdf Kellerer, Albrecht M. ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9037/1/9037.pdf Kellerer, Albrecht M.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9042/1/9042.pdf Kellerer, Albrecht M. ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9084/1/9084.pdf Scriba, Peter Christian ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9106/1/9106.pdf Zwißler, Bernhard ddc:610, Medizin

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9135/1/9135.pdf Scherb, Wolfgang; Gall, Helmut; Bähren, Wolfgang; Stief, Christian Georg ddc:610, Medizin

Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines and one neuroblastoma cell line. The number of spots detected in interphase nuclei of these tumor cell lines and in normal diploid nuclei correlated well with metaphase counts of chromosomes specifically labeled by in situ hybridization. Rapid and reliable assessments of aneuploid chromosome numbers in tumor lines in double hybridization experiments were achieved, and rare cells with bizarre phenotype and chromosome constitution could be evaluated in a given tumor cell population. Even with suboptimal or rare chromosome spreads specific chromosome aberrations were delineated. As more extensive probe sets become available this approach will become increasingly powerful for uncovering various genetic alterations and their progression in tumor cells.

Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension. Streptavidin was covalently coupled to the surface of magnetic beads and these were incubated with the hybridized chromosomes. The human chromosomes were bound to the magnetic beads through the strong biotin-streptavidin complex and then rapidly separated from nonlabeled Chinese hamster chromosomes by a simple permanent magnet. The hybridization was visualized by additional binding of avidin-FITC (fluorescein) to the unoccupied biotinylated human DNA bound to the human chromosomes. After magnetic separation, up to 98% of the individual chromosomes attached to magnetic beads were classified as human chromosomes by fluorescence microscopy.

A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported. DNA inserts from a single chromosomal library are labeled with biotin and partially preannealed with a titrated amount of total human genomic DNA prior to hybridization with cellular or chromosomal preparations. The cross-hybridization of repetitive sequences to nontargeted chromosomes can be markedly suppressed under appropriate preannealing conditions. The remaining single-stranded DNA is hybridized to specimens of interest and detected with fluorescent or enzymelabeled avidin conjugates following post-hybridization washes. DNA inserts from recombinant libraries for chromosomes 1, 4, 7, 8, 13, 14, 18, 20, 21, 22, and X were assessed for their ability to decorate specifically their cognate chromosome; most libraries proved to be highly specific. Quantitative densitometric analyses indicated that the ratio of specific to nonspecific hybridization signal under optimal preannealing conditions was at least 8:1. Interphase nuclei showed a cohesive territorial organization of chromosomal domains, and laserscanning confocal fluorescence microscopy was used to aid the 3-D visualization of these domains. This method should be useful for both karyotypic studies and for the analysis of chromosome topography in interphase cells.

Chromosome aberrations in two glioma cell lines were analyzed using biotinylated DNA library probes that specifically decorate chromosomes 1, 4, 7, 18 and 22 from pter to qter. Numerical changes, deletions and rearrangements of these chromosomes were radily visualized in metaphase spreads, as well as in early prophase and interphase nuclei. Complete chromosomes, deleted chromosomes and segments of translocated chromosomes were rapidly delineated in very complex karyotypes. Simultaneous hybridizations with additional subregional probes were used to further define aberrant chromosomes. Digital image analysis was used to quantitate the total complement of specific chromosomal DNAs in individual metaphase and interphase cells of each cell line. In spite of the fact that both glioma lines have been passaged in vitro for many years, an under-representation of chromosome 22 and an over-representation of chromosome 7 (specifically 7p) were observed. These observations agree with previous studies on gliomas. In addition, sequences of chromosome 4 were also found to be under-represented, especially in TC 593. These analyses indicate the power of these methods for pinpointing chromosome segments that are altered in specific types of tumors.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9328/1/9328.pdf Cremer, Christoph; Cremer, Thomas ddc:6

Plasmid clones containing up to 94 kilobases of single-copy DNA from band q22.3 of chromosome 21 and a complete pool of insert DNA from a chromosome 21 recombinant library have been used to rapidly detect numerical and structural aberrations of chromosome 21 by in situ hybridization in both metaphase and interphase cells. A trisomic karyotype, diagnostic of Down syndrome, is readily detected in nonmitotic cells because the majority of their nuclei exhibit three discrete foci of hybridization, in contrast to normal diploid cells, which show two foci. Chromosomal translocations involving chromosome 21 sequences were also detected with these probes, and the intranuclear location of 21q22.3 DNA sequences in "normal" human brain neurons was established with the plasmid DNA probe set. These results suggest that chromosome 21-specific probes may have utility in clinical diagnostics, especially by facilitating the direct analysis of interphase cells.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9332/1/9332.pdf Jochum, Marianne; Inthorn, D.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9334/1/9334.pdf Fritz, Hans; Jochum, Marianne ddc:610, Medizin 0

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9336/1/9336.pdf Richter, J. A.; Jochum, Marianne; Spannagl, Michael; Blümel, G.; Sternberger, A.; Wendt, P.; Baranky, A.; Dietrich, W.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9337/1/9337.pdf Kortmann, H.; Jochum, Marianne; Fröhlich, D.; Billing, A.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/4358/1/4358.pdf Hilpert, Konrad ddc:610, Medizin, Katholische Theologie

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5375/1/Zimmermann_Wolfgang_5375.pdf Thompson, John A.; Kleist, Sabine von; Shively, John E.; Fiebig, Heinz-Herbert; Schempp, Werner; Rudert, Fritz; Ortlieb, Barbara; Weber, Bernhard; Zimmermann, Wolfgang

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5385/1/Zimmermann_Wolfgang_5385.pdf Shively, John E.; Riggs, Arthur D.; Hinoda, Yuji; Shively, Louise; Zimmermann, Wolfgang; Neumaier, Michael

To study the stimulating effect of adrenaline (ADR) on active Na+/K+ transport we used double-barrelled ion-sensitive micro-electrodes to measure the activities of extracellular K+ (aKe) and intracellular Na+ (aNai) in isolated preparations of rat soleus muscle, normal human intercostal muscle and one case of hyperkalemic periodic paralysis (h.p.p.). In these preparations bath-application of ADR (10−6 M) resulted in a membrane hyperpolarization and transient decreasesaKe andaNai which could be blocked by ouabain (3×10−4 M). In the h.p.p. muslce a continuous rise ofaNai induced by elevation ofaKe to 5.2 mM could be stopped by ADR. In addition, the intracellular K+ activity (aKi), the free intracellular Ca2+ concentration (pCai) and intracellular pH (pHi) were monitored in rat soleus muscle. During ADRaKi increased, pHi remained constant and intracellular Ca2+ apparently decreased. In conclusion, our data show that ADR primarily stimulates the Na+/K+ pump in mammalian skeletal muscle. This stimulating action is not impaired in the h.p.p. muscle.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5757/1/5757.pdf Paumgartner, Gustav; Riedel, Angelika; Knorr, H. J.; Xie, Yining; Arendt, Rainer M.; Gerbes, Alexander L.

The use of tumour associated antigens in the diagnosis of serous effusions was studied in 76 patients with benign and 200 patients with malignant disease. Tissue polypeptide antigen (TPA), alpha fetoprotein, and CA 125 were found to be of little value. At cut off points of 3 ng/ml, 10 U/ml, and 30 U/ml, respectively, carcinoembryonic antigen (CEA), biliary glycoprotein I (BGP I), and CA 19-9 discriminated between benign and malignant serous effusions with a sensitivity of between 24% and 67%. The immunocytochemical staining for these markers resulted in malignant cells being detected in 18% to 33% of cases. Various combinations of conventional cytological examination, effusion fluid tumour marker determination, and immunocytochemical analysis identified malignant cells in serous effusions in up to 72% of cases; conventional cytology alone detected tumour cells in only 30%.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5769/1/5769.pdf Wernze, H.; Paumgartner, Gustav; Jüngst, Dieter; Schnizer, W.; Gerzer, R.; Arendt, Rainer M.; Gerbes, Alexander L.

Fri, 1 Jan 1988 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5885/1/5885.pdf Arendt, Rainer M.; Xie, Yining; Vollmar, Angelika M.; Gerbes, Alexander L. ddc:610, Mediz

Atrial natriuretic factor (ANF) N-terminal (ANF 1–98) and C-terminal (ANF 99–126) fragments were determined by radioimmunoassay in human plasma. Mean basal plasma ANF N-terminal concentrations in 9 healthy subjects were 461 ± 58 fmol/ml,significantly (p