Podcasts about ahls

Welcome to episode 57 of The Ski Instructor Podcast, this week featuring a return to the pod for Jon and Tom. Jon and Tom have gone in a new direction with their own ski instructor training company, Level Up Ski Coaching.  You can find them here: www.levelupskicoaching.com Insta and FB @levelupskicoaching In this episode we chat all things to do with their new venture, ski instructor cars and accommodation, off season training and skiing robots. On the day that we recorded this it was about 33 degrees in Le Châble and we had a fan on in the background so that is what the background white noise is. I hope that you enjoy the episode as much as I did making it. Dave dave@snow-pros.ski www.snow-pros.ski music by www.bensound.com

- Trong chuyến hải trình vượt hơn 1.000 hải lý đi thăm, tặng quà, động viên quân, dân huyện đảo Trường Sa và Nhà giàn DK1, các Đoàn công tác đã tổ chức Lễ tưởng niệm các cán bộ, chiến sĩ Hải quân đã anh dũng hy sinh trong khi làm nhiệm vụ trên biển. Lễ tưởng niệm được diễn ra ngay trên tàu tại vùng biển Cô Lin – Len Đao – Gạc Ma (Quần đảo Trường Sa) hay trên vùng biển DK1 trên thềm lục địa phía Nam của Tổ quốc, giữa biển khơi với lòng thành kính biết ơn và tiếc thương vô hạn những anh hùng liệt sỹ (AHLS) đã nằm lại biển sâu, nhưng các anh còn để lại mãi tinh thần và khí phách của người lính giữ đảo. Chủ đề : Tri ân liệt sỹ Trường Sa, tinh thần và khí phách, người lính giữ đảo --- Support this podcast: https://podcasters.spotify.com/pod/show/vov1sukien/support

Mintglass, Tampongtestare & Herr Ahls Kappor! by Lena & Susann Algeskär

I denna bonusepisod får vi lära känna Pål Ahlsén som är VD för fastighetsbolaget Akelius Residential. Bolaget har ju ingen vanlig stamaktie noterad men däremot finns deras preferensaktie på First North och de håller i dagarna på att emittera en D-aktie. I episoden får vi lära känna Pål och lära oss mer kring Akelius, Dessutom pratar vi om hyresregleringar och hur de kan skapa vallgravar, varför sämre ekonomiska tider kan vara av godo, vad en typisk Akeliusfastighet är och mycker mer. Dessutom berättar Pål om D-aktien och vad som skiljer den mot preferensaktien, varför den denomineras i euro och hur man ska se på det faktum att risken för innehavaren på marginalen ökar i preferensaktien jämfört med D-aktien. Det finns även goda skäl att ge ut D-aktier då de räknas som eget kapital jämfört med preferensaktiens hybridmodell vilket kanske ger bättre rating, hur påverkar detta bolaget? Kommer de lösa in preferensaktien och finns det några mörka moln på fastighetshimlen? Det och mycket mycket mer! En delikat lyssning tillönskas, Nicklas

Flygande tefat, Skansen-romantik, förortstristess och Robin Hood-borgar från 1300-talet. En blandning som heter duga. Men hur ska mönsterstaden Örebro undvika att bli en monsterstad? Örebro en flack rutnätsstad med medeltida anor. En stad Mark Isitt anser inte hade varit jättespännande om det inte vore för Svartån som dundrar fram längst slottet med sina bulliga torn, månghundraårig trähusbebyggelse i faluröd, pråliga jugendhus och strama funkiskåkar. Det är missmatch men med absolut gehör. Allt hålls ihop av detta magnifika slott. Mark Isitt I centrum finns också Medborgarhuset ett utflyktsmål för arkitekturälskare, ritat av Erik och Tore Ahlsén. Fyra våningar som staplats snett där varje våning har sin egen fasad. Men värst medborgarvänligt är det då inte utan nästan omöjligt att komma in i. Inte långt ifrån ligger bröderna Ahlséns andra skapelse Krämaren, med både bostäder och shopping. En koloss stor som ett kvarter - med två bostadshus på 13 våningar i armégrönt. Krämaren ett stort långfinger till resten av stan eller rättare sagt två långfingrar, säger Mark Isitt. Men utanför centrum finns det områden som inte riktigt passar in i Örebros identitet som mönsterstad. Vivalla och Baronbackarna två områden där arkitekturen inte nått ända fram. När Mark Isitt vandrar runt i Baronbackarna ser han flerbostadshus med tre till fyra våningar och ödsliga gräsmattor separerade av ödsliga parkeringsplatser. Byggda enligt en slags löpande band-arkitektur.  Herrejösses vilken monotoni här är alltså. Det är inte fult, det är gediget byggda hus, men här är bara så oändligt trist. Mark Isitt Hör hela Stadsinspektionen från Örebro i ljudklippet Stadsinspektionen stadsinspektionen@sverigesradio.se

Bacteria constantly need to monitor their environment and adapt the bacterial group-coordinated behaviour to changing habitats like nutrition alterations or host variations. Commonly cell-cell communication via acyl homoserine lactones (AHLs)is used to synchronise the behaviour of a bacterial population dependent on cell size. This process is referred to as quorum sensing (QS) and predominantly occurs in Gram-negative bacteria. The typical QS system consists of a LuxI-synthase that synthesises AHLs, and a LuxR-type receptor, which then responds to these AHLs. Upon AHL-binding, the LuxR-type receptor regulates the expression of different target genes and thus influences several processes, like biofilm formation, virulence, antibiotic production or cell-cell interaction. Interestingly, many proteobacteria possess additional LuxR homologs, but lack a cognate LuxI-type synthase. Those LuxR-type receptors are referred to as LuxR orphans or LuxR solos and can expand the regulatory QS network. Photorhabdus species are insect pathogenic bacteria, living in symbiosis with entomopathogenic nematodes. They all possess an exceptionally high number of LuxR solos, but lack LuxI homologs and therefore do not produce AHLs. The function of these LuxR solos, their role in cell-cell communication and the identification of their cognate signalling molecules in Photorhabdus species is the main focus of this work. In this thesis a novel signalling molecule used for QS could be identified for the first time in P. luminescens. This novel QS molecule is an α-pyrone named photopyrone (PPY) and produced endogenously by the photopyrone synthase (PpyS). The PPYs are specifically recognized by the LuxR solo regulator PluR, which then activates expression of the pcf (Photorhabdus clumping factor) operon leading to cell clumping of P. luminescens cells. Moreover, the PpyS/PluR quorum sensing system and its induced cell clumping contribute to the overall toxicity of P. luminescens. Furthermore, a second novel signalling molecule sensed by a LuxR solo of Photorhabdus species could be identified besides PPYs. The insect and human pathogenic bacteria P. asymbiotica lacks a PpyS homolog as well as a LuxI homolog, but harbours a pcf operon and a homologue to PluR, which is named PauR. The signalling molecule sensed by the LuxR-type receptor PauR could be identified, which is neither an AHL nor a PPY. PauR recognises a 2,5-dialkylresorcinol (DAR) produced by the DarABC pathway. Upon binding of the cognate signalling molecule, Summary XII PauR activates expression of the pcf operon. This also leads to cell clumping in P. asymbiotica. Furthermore, the DarABC/PauR QS system also contributes to the overall pathogenicity of P. asymbiotica against Galleria mellonella insect larvae. A bioinformatics approach revealed a high number of LuxR solos present in P. temperata and P. asymbiotica like in P. luminescens. Thereby, several conserved motives of amino acids could be identified, which are potentially important for signalbinding and -specificity. Variations in these amino acid motifs are assumed to reflect the overall variety of signals that can be sensed by LuxR solos. Furthermore, the specificity of the two LuxR solos PluR and PauR towards their cognate signalling molecules, PPYs and DARs, respectively, was analysed. Thereby, it could be shown that the previously identified conserved amino acid motives in the signal-binding domain (SBD), the TYDQCS-motif of PluR and the TYDQYI-motif of PauR, are essential but not sufficient for ligand-binding. Similar as for AHLs, it was unclear how the signalling molecules PPYs and DARs can cross the bacterial cell membrane. In the last part of this thesis the import mechanism for the Photorhabdus-specific signalling compounds PPYs and DARs were identified. Initial evidence could be provided that the membrane-integrated transporter FadL is mainly involved in the import of these hydrophobic compounds, and that they are not transported via simple diffusion across the cell membrane, which is assumed for AHLs. In conclusion, the data that is compiled presents two LuxR solos of Photorhabdus species adapted to sense and respond to novel non-AHL signalling molecules used for QS. Therefore, this thesis reveals that cell-cell communication via LuxR-type receptors goes far beyond AHL-signalling in nature.

X-Vertigo presents episode #002 of 'After Hours Lounge Sessions' Aka AHLS - Featuring the best in: Tech house,Progressive Techno and Deep House. Saturday December 10th 2011 X-Vertigo will be hosting a 3hrs DJ Live Set @ Seyhoun Gallery (9007 Melrose Avenue West Hollywood, CA 90069) more info at: www.x-vertigo.com

Quorum Sensing (QS) is a process that enables bacteria to communicate via chemical signalling molecules, which are called autoinducers (AI). When a threshold concentration of QS molecules is reached, the bacteria start their QS regulated gene expression e.g. bioluminescence (Vibrio fischeri), virulence factor secretion (Pseudomonas aeruginosa), biofilm formation (Burkholderia cepacia), sporulation, and mating. It was found that many Gram-negative bacteria use acylated homoserine lactones (AHLs or HSLs) as autoinducers. Due to the broad biological functions of HSLs, the interest in detection and analysis of HSLs is increasing for medical, biotechnological and agricultural applications. In the past years, numerous analytical methods have been developed for HSLs. Conventional analysis, which usually combines chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) has been very successfully applied for identification and quantification of HSLs. But normally, conventional analysis requires many steps of sample preparation, e.g. extraction, pre-concentration and optimisation of conditions to separate individual HSL molecules. In addition, many sensitive bioreporter assays have been developed using different LuxR responsive promoters, which contain LuxR family functional proteins but lack the HSL synthase. A combination of different bioassays is strongly recommended, since no bioreporter is sensitive to all HSLs. Alternatively, in this study, an anti-HSL antibody based immunochemical detection method has been successfully developed and established. HSL molecules consist of a homoserine lactone ring and an acyl side chain (4-18 carbon atoms), and they differ only from side chain length and substitution at C3 atom. Regarding the variation of the molecule structures, four HSL haptens, named HSL1, HSL2, HSL3 and HSL4, were designed for antibody and assay development. HSL1 and HSL3 have a long chain (C11-COOH), but HSL1 has an -oxo and HSL3 has an –OH functional group at the C3 position. In comparison, HSL2 (C5-COOH) and HSL4 (C9-COOH) have shorter side chains and no substitution on the C3 atom. The haptens were synthesised and were covalently coupled to the C-terminal COOH-group of the NH2-residues (lysines) of the carrier proteins (BSA/OVA). Using these HSL hapten-conjugates, rat and mouse anti-HSL monoclonal antibodies (mAbs) were produced, screened and further characterised with enzyme-linked immunosorbent assays (ELISAs). Corresponding to hapten structures, the antibodies showed different selectivities to HSLs with different substitution on C3 position and chain length. Eight mAbs (HSL1-1A5, HSL1-8E1, HSL1/2-2C10, HSL1/2-4H5, HSL4-4C9, HSL4-5H3, HSL4-5E12 and HSL4-6D3) were selected from about 200 mAbs and characterised in detail using coating antigen and enzyme tracer formats. It was demonstrated that the new assays have HSL detection ranges from nM to low µM, which is sensitive enough for detection of HSLs in natural samples according to literature. Interestingly but not surprisingly, AHLs mAbs have at least 20 times higher sensitivity against hydrolysed HSLs (named HSs) than original HSLs, because the conjugation and immunisation conditions, e.g. pH and temperature, for mAb development resulted in HSL hydrolysis. This property of antibodies additionally offers a new sensitive method to detect quorum quenching (QQ) relevant homoserines (HSs), which are important degradation products of HSLs. Comparable results have been obtained by Biacore and Aqua-Optosensor biosensors for HS (L) characterisation. Based on these properties of the mAbs, a detection method of HSLs and HSs in biological samples has been developed and optimised. With the comparison of the real samples before and after hydrolysis treatment, the assays could simply present the relative HSL- and HS- contents in the samples. Similar to bio-reporters, the identification or quantitation of single HSL molecules is not possible only using immunoassay due to the broad recognition of HSLs and HSs. For this purpose, a combination with conventional chemical analysis is a must. However, as a novel sensitive HSL and HS detection method, the developed immunoassays have the advantages of being fast, cost effective and having low sample volume requirement. Using the direct or indirect fluorescence signals of fluorophore labelled anti-HSL mAbs, the in situ experiments with modified Burkholderia cepacia biofilm on ibidi slide (plastic flow chamber from ibidi GmbH) and Pseudomonas putida inoculated barley root have been carried out. Unfortunately, the in situ tests were not successful, mainly due to remaining unspecific background signals. Nevertheless, a few steps, e.g. fluorophore labelling, biofilm formation, and surface blocking have been optimised. The door of HSL in situ tests with the antibodies is still open, if a suitable specific visualising detection method could be found in the future. Certainly, the antibodies can also be broadly applied for many other immunochemical techniques, such as immunosensors or immunoaffinity columns for characterisation or pre-screening of HSLs/HSs, as have been demonstrated successfully.

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