Medizin - Open Access LMU - Teil 02/22

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Die Universitätsbibliothek (UB) verfügt über ein umfangreiches Archiv an elektronischen Medien, das von Volltextsammlungen über Zeitungsarchive, Wörterbücher und Enzyklopädien bis hin zu ausführlichen Bibliographien und mehr als 1000 Datenbanken reicht. Auf iTunes U stellt die UB unter anderem eine…

Ludwig-Maximilians-Universität München

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Evidence that substance P does not mediate slow synaptic excitation within the myenteric plexus

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ELECTRICAL stimulation of presynaptic fibres to the so-called AH1 or type II2 myenteric neurones in guinea pig small intestine evokes a slow excitatory postsynaptic potential (e.p.s.p.) characterised by long-lasting depolarisation associated with increased membrane resistance and augmented excitability3. Two substances have been implicated as possible neurotrans-mitters for the slow e.p.s.p. Katayama and North reported that application of substance P to myenteric neurones mimicked the slow e.p.s.p.4, and J.D.W. and C.J.M. presented several lines of evidence for serotonin as the transmitter substance5,6. We now report that methysergide, a drug which abolishes both the slow e.p.s.p. and the action of exogenous serotonin5,6, does not affect the action of substance P on guinea pig myenteric neurones. The results suggest that substance P is unlikely to be the neuro-transmitter which mediates the slow e.p.s.p.

Narkosezwischenfälle aus rechtsmedizinischer Sicht

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Mon, 1 Jan 1979 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6266/1/6266.pdf Tröger, H. D.; Eisenmenger, Wolfgang ddc:610, Medizin

Untersuchungen zum Nachweis von ABO-Gruppensubstanzen an angebissenen Äpfeln

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Mon, 1 Jan 1979 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6268/1/6268.pdf Eisenmenger, Wolfgang; Tröger, H. D. ddc:610

Urinary cholesterol excretion in men with benign prostatic hyperplasia and carcinoma of the prostate

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Urinary excretion of nonesterified (NEC) and total cholesterol (TC) has been investigated in 79 patients with prostatic adenoma (BPH) and 48 patients with carcinoma of the prostate. Normal range of NEC was determined in 62 healthy individuals and was found to be 0.26-2.2 mg/24 hours (2 SD) with a mean value of 0.76 mg/24 hours. TC ranged from 0.3-2.9 mg/24 hours with a mean value of 0.92 mg/24 hours. In benign prostatic hyperplasia normal values for NEC and TC were determined in stages without residual urine. In contrast patients with BPH and residual urine showed elevated NEC in 42.1% and elevated TC in 61.4%. In prostatic carcinoma NEC hyperexcretion was present in 44.8% of stage A and B and in 52.6% of stage C and D. TC was elevated in 52.2% and 63.2%, respectively. Simultaneous determination of NEC and serum acid phosphatase (SAP) gave a pathologic result of at least one parameter in 51.7% of stage A and B and in 84.2% of stage C and D. If TC and serum acid phosphatase were combined, percentage of elevated values increases to 62.1 of stage A and B and was identical with 84.2 of stage C and D. If BPH with residual urine and other known conditions of urinary cholesterol hyperexcretion in males, pluripotential malignant testicular neoplasms and diseases of kidney and urinary tract, can be excluded with reasonable certainty, both parameters may be of value in diagnosis of prostatic cancer in even early clinical stages of the disease, especially in combination with the determination of SAP.

Comparative evaluation of nonesterified and total urinary cholesterol in papilloma and carcinoma of the bladder

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Urinary excretion of nonesterified (NEC) and total cholesterol (TC) has been determined in 137 healthy individuals with a range (2 SD) of 0.26-2.2 mg/24 hours NEC and 0.3-3.0 mg/24 hours TC. 264 patients with various internal diseases revealed normal values of NEC and TC; neoplasias, diseases of the kidney and prostatic adenoma with residual urine had been excluded with reasonable certainty. There was no correlation between urinary cholesterol excretion and cholesterol plasma levels. In papilloma of the bladder (n = 16) NEC hyperexcretion was present in 56%; elevated levels of TC were determined in 68%. In carcinoma of the bladder, T1N0M0-T2N0M0 (n = 28), hyperexcretion of NEC occurred in 50% and of TC in 64%. In advanced clinical stages of the disease, T3N0M0-T4N4M1d (n = 21), elevated values of NEC were detected in 76% and of TC in 90%. Since all macroscopically blood contaminated urinary samples had been excluded, a determination of total urinary cholesterol excretion may be valuable in the diagnosis of papilloma or carcinoma of the bladder in the absence of macrohematuria. Occult blood in urine was present in 33 of the 65 patients with papilloma or carcinoma of the bladder, which was associated in 26 cases with elevated urinary total cholesterol. 32 patients revealed a negative test for occult blood in urine. In 22 of these, hyperexcretion of urinary total cholesterol was observed, indicating diagnostic sensitivity of this parameter for papilloma and carcinoma of the bladder even in the absence of microhematuria. However, one has to regard that elevated urinary cholesterol levels could also occur in other carcinomas of the urogenital system, prostatic adenoma with residual urine and kidney diseases.

Vergleichende Untersuchung der endokrinen Ophthalmopathie mittels Ultrasonographie, Computertomographie und Fischbioassay

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Bei 35 Patienten mit endokriner Ophthalmopathie (eO) wurde zu den Parametern der Schilddrüsenfunktion (T3, T4, TBI, TRH-Test), dem Szintigramm und der Bestimmung der Schilddrüsenantikörper ergänzend die Ultrasonographie (A-scan) und Computertomographie (CT) der Orbita, sowie der Nachweis exophthalmogener Serumaktivität im Fischbioassay durchgeführt. Charakteristische Sonogramme für eine eO fanden sich in 26 Fällen. Die CT ergab bei 24 von 33 Patienten die Verdickung der musculi recti mediales und/oder der musculi recti laterales, sowie bei 17 Patienten eine Verdichtung im Bereich der Orbitaspitze. Im retrobulbären Bindegewebe zeigte sich nach Kontrastmittelgabe keine signifikante Dichtezunahme. Mit beiden Verfahren zusammen waren nur bei 2 Patienten die Kriterien einer eO nicht erfüllt. Die exophthalmogene Serumaktivität wurde in der IgG-Fraktion im Fischbioassay nachgewiesen; die Trefferquote war mit 69% relativ hoch. Zur Diagnostik der eO kann jedoch der Fischbioassay nicht empfohlen werden.

Der sogenannte Kunstfehler in der Chirurgie

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Mon, 1 Jan 1979 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6433/1/6433.pdf Spann, W.; Eisenmenger, Wolfgang ddc:610, Medizin

Biogenesis of Cytochrome c in Neurospora crassa

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# 1. Precipitating antibodies specific for apocytochrome c and holocytochrome c, respectively, were employed to study synthesis and intracellular transport of cytochrome c in Neurospora in vitro. # 2. Apocytochrome c as well as holocytochrome c were found to be synthesized in a cell-free homogenate. A precursor product relationship between the two components is suggested by kinetic experiments. # 3. Apocytochrome c synthesized in vitro was found in the post-ribosomal fraction and not in the mitochondrial fraction, whereas holocytochrome c synthesized in vitro was mainly detected in the mitochondrial fraction. A precursor product relationship between postribosomal apocytochrome c and mitochondrial holocytochrome c is indicated by the labelling data. In the microsomal fraction both apocytochrome c and holocytochrome c were found in low amounts. Their labelling kinetics do not suggest a precursor role of microsomal apocytochrome c or holocytochrome c. # 4. Formation of holocytochrome c from apocytochrome c was observed when postribosomal supernatant containing apocytochrome c synthesized in vitro was incubated with isolated mitochondria, but not when incubated in the absence of mitochondria. The cytochrome c formed under these conditions was detected in the mitochondria. # 5. Conversion of labelled apocytochrome c synthesized in vitro to holocytochrome c during incubation of a postribosomal supernatant with isolated mitochondria was inhibited when excess isolated apocytochrome c, but not when holocytochrome c was added. # 6. The data presented are interpreted to show that apocytochrome c is synthesized on cytoplasmic ribosomes and released into the supernatant. It is suggested that apocytochrome c migrates to the inner mitochondrial membrane, where the heme group is covalently linked to the apoprotein. The hypothesis is put forward that the concomitant change in conformation leads to trapping of holocytochrome c in the membrane. The probles of permeability of the outer mitochondrial membrane to apocytochrome c and the site and nature of the reaction by which the heme group is linked to the apoprotein are discussed.

Rapid sensitive and reproducible assays for human parathyrin

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9251/1/9251.pdf Scriba, Peter Christian; Marschner, I.; Kuflicki, G.; Wood, W. G. ddc:610, Medizin

Demonstration of acrosin in mouse spermatozoa

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9748/1/9748.pdf Fink, Edwin ddc:610, Medizin

Development of a Radioimmunoassay for Pig Pancreatic Kallikrein

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9749/1/9749.pdf Güttel, Christine; Fink, Edwin ddc:610, Medizin

Development of a Radioimmunoassay for Pig Pancreatic Kallikrein and its Application in Physiological Studies

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9750/1/9750.pdf Güttel, Christine; Seifert, J.; Fink, Edwin

Spermatozoa Acrosin and Seminal Plasma Acosin Inhibitors

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/9751/1/9751.pdf Fritz, Hans; Schleuning, W. D.; Fink, Edwin ddc:610, Medizin

Rechtliche Situationen der praxiseigenen Laboratorien

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/10494/1/10494.pdf Badura, Peter ddc:610, Medizin, Jura

On the mechanism of action of the cytostatic drug anguidine and of the immunosuppressive agent ovalicin, two sesquiterpenes from fungi

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/5370/1/5370.pdf Zimmermann, Wolfgang; Weiner, E. M.; Richter, H.; Hartmann, G. R.

Altersbestimmung an Spermaspuren

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6224/1/6224.pdf Eisenmenger, Wolfgang; Tröger, H. D. ddc:610, Medizin

Ein ungewöhnlicher postoperativer Zwischenfall

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6227/1/6227.pdf Wilske, J.; Schuck, M.; Eisenmenger, Wolfgang ddc:610, Medizin

Ergebnisse von "Kunstfehlergutachten"

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6229/1/6229.pdf Liebhardt, E.; Eisenmenger, Wolfgang ddc:610, Medizin

Befragung zum Rausch

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6233/1/6233.pdf Liebhardt, E.; Eisenmenger, Wolfgang; Schuck, M. ddc:610, Medizin 0

Zur histologischen und histochemischen Altersbestimmung gedeckter Hirnrindenverletzungen

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6264/1/6264.pdf Neuhann, T.; Diem, G.; Gilg, T.; Eisenmenger, Wolfgang

Wertigkeit der Pulswellenerscheinungszeit bei Störungen der Schilddrüsenfunktion

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6343/1/6343.pdf Jüngst, Dieter; Wittmer, K.; Lüderitz, B. dd

Beiträge zur rechnerunterstützten Diagnostik II

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6344/1/Juengst_Dieter_6344.pdf Knedel, M.; Jüngst, Dieter; Broda, S.; Vogt, W.; Sandel, P. ddc:610, Medizin

Fusion of Sendai virus with the target cell membrane is required for T cell cytotoxicity

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INFECTION of mice with viruses can generate cytotoxic T lymphocytes (CTL) which show restricted specificity for target cell lysis. Specific lysis requires that the virus used to prime the target cells must be of the same type as that used to sensitise the CTL, and that both target and CTL cells must express the same major histocompatability complex (MHC) gene product(s). The nature of the viral gene product(s) and their interaction with the MHC gene product(s) have been the subject of recent stud1−5. Previously we used Sendai virus to show that lysable target cells can be obtained using membrane vesicles which contain only the viral glycoproteins, indicating that these may be the specific viral gene products involved in target formation5. Sendai virus contains two glycoproteins—the haemagglutinin-neuraminidase (HANA) which promotes attachment of virus to cells and the fusion protein (F) which is involved in subsequent virus cell fusion7−9. Both activities are necessary for insertion of these viral glycoproteins into the plasma membrane of the cell10. In this letter we suggest that the insertion of the viral glycoproteins into the cell membrane is an essential step in target cell formation since we can show that virus containing an inactive fusion protein precursor (F0) cannot elicit T cell cytotoxicity unless the fusion activity is generated by proteolytic cleavage of the precursor. Sugamura et al. 6 have suggested that it is primarily the F glycoprotein of the Sendai virus envelope which is essential for the formation of the target antigen, as virus lacking the functional activities of F following trypsin digestion was inactive in priming target cells for T cell killing. However, we show that proteolytic inactivation of either of the two glycoproteins (F or HANA) of virus used to prime target cells will abolish the cytotoxic response.

Zur prophylaktischen Gabe von Vitamin D2 und D3 bei Anfallskranken

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6966/1/6966.pdf Prager, P.; Ritz, E.; Schmidt-Gayk, Heinrich; Bohn, T.; Krause, Klaus-Henning ddc:610, Medizin

Beziehungen zwischen elektromyographischen, klinisch-chemischen und klinischen Befunden bei der latenten Tetanie

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/6968/1/6968.pdf Schmidt-Gayk, Heinrich; Krause, Klaus-Henning

Fusion of isolated myoblast plasma membranes

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Fusion of plasma membranes isolated from myoblasts grown in culture has been investigated. 1. 1. Membrane fusion was specifically dependent on Ca2+ at physiological concentrations. However, at higher concentrations of cations, fusion could be triggered not only by Ca2+, but by Mg2+ and Sr2+ as well. 2. 2. The amount of fusion was directly proportional to temperature. 3. 3. Fusion was found to depend on the state of maturation of the myoblast membranes. 4. 4. Experiments with chemically and enzymatically modified membranes and with membranes derived from myoblasts grown in the presence of inhibitors of protein biosynthesis suggest the participation of proteinaceous membrane components in the fusion mechanism.

Fusion of secretory vesicles isolated from rat liver

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Secretory vesicles isolated from rat liver were found to fuse after exposure to Ca2+. Vescle fusion is characterized by the occurrence of twinned vesicles with a continuous cleavage plane between two vesicles in freeze-fracture electron microscopy. The number of fused vesicles increases with increasing Ca2+-concentrations and is half maximal around 10–6 m. Other divalent cations (Ba2+, Sr2+, and Mg2+) were ineffective. Mg2+ inhibits Ca2+-induced fusion. Therefore, the fusion of secretory vesiclesin vitro is Ca2+ specific and exhibits properties similar to the exocytotic process of various secretory cells. Various substances affecting secretionin vivo (microtubular inhibitors, local anethetics, ionophores) were tested for their effect on membrane fusion in our system. The fusion of isolated secretory vesicles from liver was found to differ from that of pure phospholipid membranes in its temperature dependence, in its much lower requirement for Ca2+, and in its Ca2+-specificity. Chemical and enzymatic modifications of the vesicle membrane indicate that glycoproteins may account for these differences.

Preparation of Neurospora crassa mitochondria

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Sun, 1 Jan 1978 12:00:00 +0100 http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7CV2-4B42H29-B4&_user=616146&_coverDate=12%2F31%2F1979&_rdoc=21&_fmt=high&_orig=browse&_srch=doc-info(%23toc%2318066%231979%23999449999%23472657%23FLA%23display%23Volume)&_cdi=18066&_sort=d&_docanc https://epub.ub.uni-muenchen.de/7323/1/7323.pdf Weiss, Hanns; Neupert, Walter; Sebald, Walter ddc:610, Medizin

Biosynthesis and intracellular translocation of mitochondrial proteins bindin protein.

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7333/1/7333.pdf Neupert, Walter; Korb, Harald; Zimmermann, Richard

Biosynthesis of mitochondrial proteins in the cytoplasm and their transport to the mitochondrial membrane

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7336/1/7336.pdf Neupert, Walter

Anatomische Besonderheiten der Wirbelsäule

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7759/1/7759.pdf Putz, Reinhard; Platzer, W. ddc:610, Medizin

Ein neues Konservierungs- und Aufbewahrungssystem für anatomisches Material

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7760/1/7760.pdf Poisel, S.; Putz, Reinhard; Platzer, W. ddc:610,

Eine seltene Form von Blockwirbelbildung im Bereich der Lendenwirbelsäule bei einer Hochleistungssportlerin

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/7761/1/7761.pdf Nedden, D. zur; Knapp, E.; Putz, Reinhard

Rapid and sensitive radioimmunoassays for human parathyrin

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8287/1/8287.pdf Scriba, Peter Christian; Marschner, I.; Wood, W. G. ddc:610, Medizin

Akute endokrine Krisen

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8288/1/8288.pdf Scriba, Peter Christian ddc:610, Medizin 0

Increased urinary excretion of triiodthyronine (T3) and thyroxine (T4) and decreased serum thyreotropic hormone (TSH) induced by motion sickness

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8289/1/8289.pdf Scriba, Peter Christian; Ulbrecht, G.; Gottsmann, M.; Erhardt, F.; Eversmann, T.; Habermann, J.

Increased secretion of growth hormone, prolactin, antidiuretic hormone, and cortisol induced by the stress of motion sickness

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8290/1/8290.pdf Scriba, Peter Christian; Werder, Klaus von; Ulbrecht, G.; Uhlich, E; Gottsmann, M.; Eversmann, T.

Treatment of patients with prolactinomas

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8291/1/8291.pdf Scriba, Peter Christian; Rjosk, H. K.; Pickardt, C. R.; Landgraf, R.; Fahlbusch, R.; Werder, Klaus von ddc:610, Medizin

Qualität und Stabilität von jodierten Speisesalzen

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8292/1/8292.pdf Scriba, Peter Christian; Jungermann, A.; Habermann, J. ddc:610, Medizin

A method for the consistent production of high quality antisera to small peptide hormones

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8294/1/8294.pdf Scriba, Peter Christian; Müller, O. A.; Madler, M.; Sofroniew, V.

Problems in automation of serum cortisol radioimmunoassay

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8295/1/8295.pdf Scriba, Peter Christian; Wood, W. G.; Müller, O. A.; Stalla, G. ddc:610, Medizin

Rapid sensitive radioimmunoassays for the C-terminal region of human parathyrin

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8296/1/8296.pdf Scriba, Peter Christian; Marschner, I.; Wood, W. G. ddc:6

Streß-induzierte Sekretionsänderungen hypophysärer Hormone

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8297/1/8297.pdf Scriba, Peter Christian; Ulbrecht, G.; Uhlich, E; Gottsmann, M.; Eversmann, T. ddc:610, Medizin

Änderung der Schilddrüsenfunktionslage bei Vestibularisreizung und bei psycho-physischen Belastungen

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8298/1/8298.pdf Scriba, Peter Christian; Ulbrecht, G.; Eversmann, T.; Habermann, J.

Die Hypophysen-Schilddrüsenregulation bei chirurgischen Schilddrüsenerkrankungen

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8299/1/8299.pdf Scriba, Peter Christian; Pickardt, C. R. dd

Stimulation der TSH-Sekretion durch TRH als Schilddrüsenfunktionstest

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8300/1/8300.pdf Scriba, Peter Christian; Horn, K.; Pickardt, C. R. ddc:610, Mediz

Prophylaxe, Diagnostik und Therapie der blanden Struma

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8302/1/8302.pdf Scriba, Peter Christian ddc:610, Medizin

Warten auf die Struma-Prophylaxe

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8303/1/8303.pdf Scriba, Peter Christian ddc:610, Medizin

Muskelschwäche als Leit- und Warnsymptom bei Endokrinopathien

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8304/1/8304.pdf Scriba, Peter Christian; Pongratz, D. ddc:610, Medizin

Einige Schwerpunkte der internistischen und neurologischen Differentialdiagnostik mit den allgemeinen klinischen Untersuchungsmethoden

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8305/1/8305.pdf Scriba, Peter Christian

Physiology of the hypothalamo-pituitary unit

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Sun, 1 Jan 1978 12:00:00 +0100 https://epub.ub.uni-muenchen.de/8306/1/8306.pdf Werder, Klaus von; Pickardt, C. R.; Scriba, Peter Christian ddc:610, Medizin

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