Regenerative Medicine and Gene Therapy

Giovanni Sorrentino, Head of Molecular Liver Pathophysiology Unit Department of Life Sciences – University of Trieste Trieste, ITALY speaks on "New avenues in disease modeling and regenerative medicine of the enterohepatic system".

Shyni Varghese, Professor & MEDx Investigator, Department of Biomedical Engineering, Mechanical Engineering and Materials Science Department of Orthopaedic Surgery, Pratt School of Engineering & Duke Medical Center, Durham, NC USA speaks on "Physicochemical properties of extracellular matrix: Key to function, Clue to mechanism". This movie has been recorded at ICGEB Trieste.

Nadja RING, Postdoc, Cardiovascular Biology lab ICGEB Trieste, ITALY speaks on "A screen is only the beginning". This seminar has been recorded at ICGEB Trieste.

Lutz Hein, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg, Germany speaks on "Dynamics of the epigenome in cardiac myocyte development and disease". This movie has been recorded at ICGEB Trieste.

Shuo Lin, Biozentrum, University of Basel, Basel, SWITZERLAND speaks on "Designed linker proteins restore basement membrane and correct LAMA2-related muscular dystrophy". This movie has been recorded at ICGEB Trieste.

Jesus Gil, Cell Proliferation Group MRC London Institute of Medical Sciences (LMS) ImperialCollege,HammersmithHospitalCampus London, UK speaks on "Linking senescence and inflammation: the senescence-associated secretory phenotype (SASP)". This movie has been recorded at ICGEB Trieste.

Eduard Ayuso, INSERM 1089, University of Nantes, Nantes, France speaks on "Unraveling mechanisms and biology of recombinant AAV vectors produced in insect cells". Recombinant adeno-associated vectors (rAAV) are viral vectors of choice for gene therapy of many inherited diseases. Medicinal products based on rAAV are predominantly manufactured by transient transfection of mammalian cells or baculovirus expressing vectors (BEV) infection of insect cells, being the latter method more suitable for large-scale production. Although AAVs are mammalian viruses they can be assembled in insect cells, but the biology of the system has been poorly investigated. Here, we have studied the role of the assembly-activating protein (AAP) in insect cells and we found that this protein is expressed in a similar manner as in mammalian cells. By knocking down the AAP, it was confirmed that AAP is required for the assembly of AAV2 particles in insect cells. Next, we aimed to identify and characterize DNA species encapsidated in rAAV stocks produced in insect cells. To this end, we developed a single-strand virus sequencing protocol based on Illumina high-throughput sequencing technology (HTS). Preliminary data obtained from rAAV stocks puri ed by CsCl ultracentrifugation or immunoaffnity chromatography revealed that baculoviral and cellular DNA correspond to ≤1.5% and ≤0.02% of the total reads, respectively. Moreover, the sequencing coverage showed that the proximity to the ITRs increases progressively the probability for baculoviral DNA to be encapsidated. Nonetheless, these baculovirus-derived reads are found at a frequency of 2-3 logs lower than the rAAV genome reads. The development of accurate quality control methods is not only critical for fulfilling regulatory requirements, but will also provide novel insights into the biological mechanism of rAAV assembly in insect cells. This movie has been recorded by ICGEB Trieste.

Fulvio MAVILIO, Genethon, Evry - FRANCE speaks on "Gene Therapy of Inherited Neuromuscular Diseases". This seminar has been recorded by ICGEB Trieste

Adi BARZEL, Founder and Chief Scientific Officer of LogicBio Therapeutics Inc. Tel Aviv - ISRAEL speaks on "Therapeutic levels of in vivo gene targeting without nucleases". This seminar has been recorded by ICGEB Trieste

Paolo Bianco Sapienza University of Rome, Rome - ITALY speaks on "Skeletal stem cells and the stromal system: moving away from the "mesenchymal" conceptual bug". This seminar has been recorded by ICGEB Trieste

Katja Schenke-Layland, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Cell and Tissue Engineering, Cardiovascular Tissue Engineering, Stuttgart - GERMANY speaks on "Extracellular matrix – A natural biomaterial for tissue engineering applications". This seminar has been recorded by ICGEB Trieste

Michela Rigoni, Dept. of Biomedical Sciences, University of Padua, Padua - ITALY speaks on "Neurotoxins-induced degeneration and regeneration of motor axon terminals". This seminar has been recorded by ICGEB Trieste

Liborio Stuppia DISPUTer, School of Medicine and Health Sciences, G. d'Annunzio University, Chieti, ITALY speaks on "Amniotic Fluid Stem Cells (AFSCs): biological properties and therapeutic applications". This seminar has been recorded by ICGEB Trieste

Slobodan Vukicevic, Laboratory of Mineralized Tissues and Proteomics, Center for Translational and Clinical Research, School of Medicine, University of Zagreb - CROATIA speaks on "Morphogenetic Proteins in regenerative medicine". This seminar has been recorded by ICGEB Trieste

Lucie Carrier, Department of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, GERMANY speaks on "RNA-based therapy for cardiac genetic disease". This seminar has been recorded by ICGEB Trieste

Federico Mingozzi, University Pierre and Marie Curie, Paris, FRANCE, Genethon, Evry, FRANCE speaks on "Overcoming immune responses to AAV vectors". This seminar has been recorded by ICGEB Trieste

Fulvio Mavilio, Genethon, Paris - FRANCE "Integrated approaches to gene therapy of genetic diseases". This seminar has been recorded by ICGEB Trieste

Matthew Taylor, Adult Clinical Genetics, Department of Medicine UCHSC, Cardiovascular Institute, University of Colorado, USA speaks on " Big Hearts and Bigger Exomes: Next Generation DNA Sequencing in Cardiomyopathies". This seminar has been recorded by ICGEB Trieste

Perikles Simon, Sports Medicine, Rehabilitation and Disease Prevention, Johannes Gutenberg-University Mainz, GERMANY speaks on " Pitfalls and Potential of Transgene-Based Approaches for the Detection of Gene Doping". This seminar has been recorded by ICGEB Trieste

Maurizio Capogrossi, Istituto Dermopatico della Immacolata, IDI, Patologia Vascolare, Rome, ITALY speaks on "HMGB1 and left ventricular remodeling after infarction". This seminar has been recorded by ICGEB Trieste

Richard Snyder, Biotherapeutic Programs, Office of Research, Department of Molecular Genetics and Microbiology, University of Florida, USA speaks on "Recombinant AAV Vectors: persistence in vivo and clinical product release". This seminar has been recorded by ICGEB Trieste

Toni Cathomen, Hannover Medical School, Experimental Hematology, Hannover, GERMANY speaks on "Zinc positive: designer nucleases in gene therapy". This seminar has been recorded by ICGEB Trieste