Podcasts about cscl

Acknowledgement of Country//Headlines//Today we heard the third instalment of Spike's conversation with Iain McIntyre from the Commons Social Change Library. Last week Iain talked about the resources available at the CSCL, who uses them and the importance of history in developing campaigns to address the present and future. In this the final segment, Iain and Spike talked about some of the social forces that work against acknowledging our radical heritage/history, and the importance of crafting counter narratives to communicate and raise awareness of our activist/critical culture. They also discussed how the CSCL's resources have been used by people, highlighting one of CSCL's main aims- to ensure that all its tools and resources are available and accessible to people of all backgrounds and abilities. They also talked about social movements, and the crucial role that the CSCL performs in providing "movement infrastructure,” a place where information and tips can be safely deposited and shared to build strong movements for change. Listen back to part 1 and part 2.//Socialist Alliance Councillor for Merri-bek Sue Bolton joined us to speak about Saturday's National Day of Action for Housing, which in Narrm/Melbourne will focus on a campaign to stop the demolition of the 44 high-rise public housing flats. Sue also talked about the Maribyrnong Council's decision to rescind its motion for peace and justice in Palestine, and about local council decision-making and the importance of resident activism. Join the rally for public housing at 1PM this Saturday the 9th of December outside the State Library of Victoria.//Academic and activist Dr Lina Koleilat came on the show to chat about the Institute of Postcolonial Studies, which has been a cultural and educational hub in Narrm for over 25 years. Lina spoke about the importance of the space as a site of activist intellectual production and sharing. There is an open meeting happening today, Thursday the 7th of December from 7-8PM, to discuss the current status of the IPCS and its future. You can join the meeting by clicking this link and find out more on the Save Postcolonial Studies Instagram page.// We played a speech given in Parliament by Independent Senator for VictoriaLidia Thorpe on the necessity of implementing the United Nations Declaration on the Rights of Indigenous Peoples or UNDRIP in Australian legislation in the wake of yesterday's majority vote against herPrivate Members' Bill to implement UNDRIP by Labor and Coalition Parliamentarians. This decision came in the wake of the Joint Standing Committee on Aboriginal and Torres Strait Islander Affairs' publication of itsreport on the inquiry into the implementation of UNDRIP in Australia in late November. Senator Thorpe is a Gunnai, Gunditjmara and Djab Wurrung mother, grandmother, activist, and politician, and is the first Aboriginal Senator from Victoria.//Songs// Disco Dal'ona - TootArd//

 Acknowledgement of Country// Headlines// The Commons Social Change Library is the "go to portal" for social change resources. They gather and share resources from many different sources and organisations from all round the world in a user friendly, accessible portal. Last week we heard part 1 of our two part conversation with Iain Mcyntyre from the CSCL where we discussed social change, why it was important to be able to access information about social change struggles in common, and how documenting struggles helps us to achieve social change in the future. I also asked Iain if we can reform our to a more socially just society, and what are the first steps in planning a campaign. This week we heard about the types of resources available at the CSCL, who uses them and the central role that history plays in planning and achieving social change.// CONTENT WARNING: This segment covers some distressing content around state violence - if you need to speak to someone, you can call Lifeline on 13 11 14, Aboriginal and Torres Strait Islander listeners can call 13 YARN, and queer listeners can call QLife on 1800 184 527.Independent queer researcher Dr Shoshana Rosenberg joins us to talk about disability and trans solidarity with Palestine in the face of Israel's ongoing violent occupation, exploring the connections between disability justice, trans freedom and liberation for Palestine. We discuss genocide as a mass disabling event and what it means for disabled folks to engage in solidarity actions across colonial borders and from stolen land in so-called Australia.// Ronny Kareni, West Papuan activist, 3CR broadcaster and advisor and honorary Fellow at the University of Wollongong's West Papua project, joins us to share some reflections on the struggle for liberation for West Papua in the lead up to December 1st, West Papuan Independence Day. December 1st marks the anniversary of the first raising of the Morning Star flag, and this year will be commemorated in Naarm/Melbourne with the Morning Star Ceremony, which kicks off at 4PM tomorrow at Federation Square.// Earlier this week, a group of Indigenous and non-Indigenous activists set up their second blockade in a month across the single access road to the Pine Gap military facility, situated on Arrernte land outside of Mparntwe/Alice Springs. Today, we are joined by Carmen, one of the activists who participated in the blockade and a member of Mparntwe for Palestine, to discuss the relationship between Pine Gap and the ongoing genocide of Palestinians, as well as the importance of direct action in solidarity with Palestinians struggling for liberation. Read the article about Pine Gap's surveillance apparatus by Peter Cronau in Declassified Australia here.//

Qui, dans sa scolarité, n'a pas dû faire un travail de groupe ? Aucun d'entre nous ! Qui s'est déjà vu expliquer pourquoi travailler en groupe ? Aucun d'entre nous non plus… Il y a un présupposé, une évidence qu'on ne questionne pas, qui voudrait que nous soyons toutes et tous plus motivé·e·s et compétent·e·s dès lors que l'on travaille à plusieurs, comme si la performance collaborative équivalait à la somme du potentiel de chacun. C'est pourtant loin d'être aussi simple. Qu'est-ce qu'un groupe ? À quelle dynamique répond-il ? Quels sont les défis qui l'accompagnent ? En existe-t-il des modélisations pédagogiques ? Comment inciter les échanges, et surtout leur donner du sens ? Ce sont toutes ces interrogations que nous mettons sur la table afin de mesurer, au final, la plus-value d'une formation avec… ou sans colaboration. On a évoqué… Le chapitre “Les élèves apprennent mieux en groupe” de l'ouvrage “L'innovation pédagogique” d'André Tricot Le marshmallow challenge Les rétrospectives AGILE Sur l'outil Collabucate se basant sur les travaux de Järvelä et al. : Lyons K.M., Lobczowski N.G., Greene J.A., Whitley J. & McLaughlin J.E., “Using a design-based research approach to develop and study a web-based tool to support collaborative learning”, Computers & Education, Volume 161, February 2021. https://doi.org/10.1016/j.compedu.2020.104064 Sur la régulation en tâche d'apprentissage collaboratif : Järvelä, S., Kirschner, P.A., Panadero, E. et al. Enhancing socially shared regulation in collaborative learning groups: designing for CSCL regulation tools. Education Tech Research Dev 63, 125–142 (2015). https://doi.org/10.1007/s11423-014-9358-1 Tuckman et sa Developmental sequence in small groups (1965) (Illustration) Elliot Aronson et la Jigsaw classroom (1971) : https://jigsaw.org/ Recommandations Jérôme : Dictionnaire Electronique des Synonymes - https://crisco2.unicaen.fr/des/ Lionel : La lecture numérique, par exemple sur Kindle Nicolas : L'émission “Collaborer et coopérer à l'école : pour quoi faire ?” sur France Culture L'application “Apple Notes” à travers deux vidéos : “Pourquoi Apple Notes est peut-être la meilleure application de prise de notes“ (ENG) et “6 astuces pour utiliser Apple Notes comme un·e pro !” (FR) Hébergé par Ausha. Visitez ausha.co/politique-de-confidentialite pour plus d'informations.

On this episode of Cultivating Second Chair Leaders, we discuss highlights from this first season of the podcast with guest Audrey Duensing-Werner (@dceaudrey).  Audrey also shares some encouragement from her own personal experience of living out Cultivating Faith practices in her own life. You will also hear part two of an interview with Dr. Bill Karpenko (@billkarpenko) as he shares application of Christ-like practices in his life, along with several resources for continued growth. Resources mentioned in today's episode: Vantage Point by Brenda Jank: https://runhardrestwell.org/resource/vantage-point-book/ A Rest That Works Series: https://runhardrestwell.org/video-series/ Download KINDLE's Strands and Practices: http://www.kindleservantleaders.org/resources/strands-practices-outcomes-and-grace-filled-marks/ New Morning Mercies by Paul David TrippReliving the Passion by Walter Wangerin Jr. A Praying Life by Paul Miller Epic by John Eldredge The Circle Maker by Mark Batterson The Deeply Formed Life, by Rich VallodasAnxious for Nothing by Max Lucado    National Association of Directors of Christian Education (NADCE) - https://nadce.wildapricot.org/  Go to www.kindleservantleaders.org  and to learn more about the organization and support our work!Connect: Instagram: @emilyphoenix or @kindle_social Facebook: https://www.facebook.com/KINDLEServantLeaders Email: emily@kindleservantleaders.org and we may share your story in the future! Share this episode with a friend and leave us a review. Share feedback about Season 1!

Eduard Ayuso, INSERM 1089, University of Nantes, Nantes, France speaks on "Unraveling mechanisms and biology of recombinant AAV vectors produced in insect cells". Recombinant adeno-associated vectors (rAAV) are viral vectors of choice for gene therapy of many inherited diseases. Medicinal products based on rAAV are predominantly manufactured by transient transfection of mammalian cells or baculovirus expressing vectors (BEV) infection of insect cells, being the latter method more suitable for large-scale production. Although AAVs are mammalian viruses they can be assembled in insect cells, but the biology of the system has been poorly investigated. Here, we have studied the role of the assembly-activating protein (AAP) in insect cells and we found that this protein is expressed in a similar manner as in mammalian cells. By knocking down the AAP, it was confirmed that AAP is required for the assembly of AAV2 particles in insect cells. Next, we aimed to identify and characterize DNA species encapsidated in rAAV stocks produced in insect cells. To this end, we developed a single-strand virus sequencing protocol based on Illumina high-throughput sequencing technology (HTS). Preliminary data obtained from rAAV stocks puri ed by CsCl ultracentrifugation or immunoaffnity chromatography revealed that baculoviral and cellular DNA correspond to ≤1.5% and ≤0.02% of the total reads, respectively. Moreover, the sequencing coverage showed that the proximity to the ITRs increases progressively the probability for baculoviral DNA to be encapsidated. Nonetheless, these baculovirus-derived reads are found at a frequency of 2-3 logs lower than the rAAV genome reads. The development of accurate quality control methods is not only critical for fulfilling regulatory requirements, but will also provide novel insights into the biological mechanism of rAAV assembly in insect cells. This movie has been recorded by ICGEB Trieste.

Tracy Eckert

Over the last decades collaborative learning has gained immensely in importance and popularity due to its high potential. Unfortunately, learners rarely engage in effective learning activities unless they are provided with instructional support. In order to maximize learning outcomes it is therefore advisable to structure collaborative learning sessions. One way of doing this is using collaboration scripts, which define a sequence of activities to be carried out by the learners. The field of computer-supported collaborative learning (CSCL) produced a variety of collaboration scripts that proved to have positive effects on learning outcomes. These scripts provide detailed descriptions of successful learning scenarios and are therefore used as foundation for this thesis. In many cases computers are used to support collaborative learning. Traditional personal computers are often chosen for this purpose. However, during the last decades new technologies have emerged, which seem to be better suited for co-located collaboration than personal computers. Large interactive displays, for example, allow a number of people to work simultaneously on the same surface while being highly aware of the co-learners' actions. There are also multi-display environments that provide several workspaces, some of which may be shared, others may be personal. However, there is a lack of knowledge regarding the influence of different display types on group processes. For instance, it remains unclear in which cases shareable user interfaces should replace traditional single-user devices and when both personal and shared workspaces should be provided. This dissertation therefore explores the role of personal and shared workspaces in various situations in the area of collaborative learning. The research questions include the choice of technological devices, the seating arrangement as well as how user interfaces can be designed to guide learners. To investigate these questions a two-fold approach was chosen. First, a framework was developed, which supports the implementation of scripted collaborative learning applications. Second, different prototypes were implemented to explore the research questions. Each prototype is based on at least one collaboration script. The result is a set of studies, which contribute to answering the above-mentioned research questions. With regard to the choice of display environment the studies showed several reasons for integrating personal devices such as laptops. Pure tabletop applications with around-the-table seating arrangements whose benefits for collaboration are widely discussed in the relevant literature revealed severe drawbacks for text-based learning activities. The combination of laptops and an interactive wall display, on the other hand, turned out to be a suitable display environment for collaborative learning in several cases. In addition, the thesis presents several ways of designing the user interface in a way that guides learners through collaboration scripts.

This article deals with cooperation scripts as a method of collaborative learning. The particular focus lies on cooperation scripts, which aim at the improvement of individual text comprehension in the classroom. In this context, different approaches and strategies applied were illustrated. Furthermore, this article presents recent trends for the implementation of cooperation scripts in CSCL scenarios.

High concentrations of lipids in ascites fluid caused by peritoneal carcinomatosis have been described recently. Since their nature has not yet been clarified, we isolated ascitic lipids from 25 patients with various neoplasms for further characterization. After chromatography on Sephadex G-100 gels, the ascitic lipids were fractionated on a Biogel A-5m column in three peaks. The second and third peaks were identified as low and high density lipoproteins, which were most likely of plasmatic origin, and represented the major amounts of ascitic lipids. The first peak was eluted in the void volume, indicating a molecular weight over 5 million. It consisted, on the average, of 65.3% protein, 16.2% triglycerides, 7.4% phospholipids, and 7.0% cholesterol. In a CsCl gradient, this protein-lipid complex floated in the density range from 1.128 to 1.181 g/ml. Sodium dodecyl sulfate: polyacrylamide gel electrophoresis separated up to 11 protein subunits (Mr 29,000 to 97,000), and electron microscopy revealed globular particles of 36 to 64 nm in diameter. The macromolecular complex showed no immunological reaction against anti-{alpha}- and anti-ß-lipoproteins, but a single precipitation line against anti-liver-specific lipoprotein was seen. The biochemical characteristics of this protein-lipid complex proved to have a close relationship to liver-specific lipoprotein. It is most likely derived from cell membranes of the peritoneum detached by carcinomatosis.

The cholesterol-containing complexes in the urine of normal subjects and patients with diseases accompanied by hyperexcretion of urinary cholesterol were characterized. In normal subjects, the major portion of the recovered urinary cholesterol was eluted in the void volume fractions after gel chromatography on Bio-Gel A-5m; this suggested an association with a macromolecular complex above 5 X 10(6) daltons. A comparable elution pattern was seen in most of the urines of the patients with benign or malignant diseases of the kidneys or the urogenital tract. However, in single patients with hyperexcretion of urinary cholesterol, considerable amounts of cholesterol were detected in the included volume of the column. This was caused by additional excretion of high density lipoproteins or both high and low density lipoproteins in the urine which could be identified in these fractions by agarose electrophoresis and immunodiffusion. These results indicate that the macromolecular complex represents the majority of the recovered urinary cholesterol in normal subjects and in disease states with known hyperexcretion. Macroscopically, the isolated cholesterol- containing complex in the void volume fractions was turbid, and electron microscopy showed lipoprotein-like particles with diameters ranging from 300 to 700 A. The chemical analysis revealed median values of protein (46.0%), triglycerides (16.3%), cholesterol (8.2%), and phospholipids (29.5%) in normal subjects and comparable results in the patients with benign or malignant diseases of the kidney and the urogenital tract. Ethanolamine glycerophospholipids, phosphatidylcholine, sphingomyelin, and phosphatidylserine were the main phospholipid components. After ultracentrifugation in a CsCl gradient, the cholesterol-containing complex was found between densities 1.1 and 1.3 g/ml. By SDS polyacrylamide electrophoresis, up to 17 protein subunits in the molecular weight range of 14,000 to 87,500 were separated. Immunodiffusion studies showed in about 40% precipitin lines against anti-human albumin, but no reactions against anti-human apoHDL and anti-human apoLDL. However, immunodiffusion of the macromolecular complex against anti-liver-specific and anti-kidney- specific lipoproteins revealed single precipitin lines. In conclusion, the isolated cholesterol-containing urinary complex showed many characteristics of membrane-associated protein-lipid particles of the human kidney and even the liver. These proteolipids are the major source of urinary cholesterol in normal and disease states.