Podcasts about B22

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Best podcasts about B22

Latest podcast episodes about B22

聖經之鑰
詩篇-第69篇-為聖殿受苦與讚美【聖經之鑰】Psalms

聖經之鑰

Play Episode Listen Later Aug 24, 2023 3:30


一、思想問題:今日是否有些壓力、苦楚?為什麼受苦?是為義、為耶穌,還是為自己、為世界? 二、鑰節: 69:9 因我為你的殿心裏焦急,如同火燒,並且辱罵你人的辱罵都落在我身上。 我們今日到底會為了什麼事情心中焦急?為自己的事?為自己的福利、愛情、金錢?大衛卻是滿心為著神的殿心中焦急。願我們體會大衛的心,即便要面對許多人的攻擊、誤會、打壓批判,但是我們仍要專心選擇神,因為這才是生命冊上會被記錄的人,是在永恆中會被神記念的事。 三、經文結構: A1-4 苦境:敵人無故地攻擊。 →B5-6 呼求:承認自罪,不叫人跌倒。 A7-12 苦境:因關心神的殿而淪為笑柄,充滿羞愧。 →B13-18 呼求:神拯救我。 A19-21 苦境:羞辱與敵人的攻擊。 →B22-28 呼求:主攻擊敵人。 →B29-36 以讚美得勝:29-31 大衛的讚美;32-33 謙卑人的讚美;34-36 眾人看見讚美。 四、結構亮光: 本篇詩篇是大衛關心聖殿,因而受到敵人的攻擊與羞辱,經文由苦境A與呼求B交替呈現。 A的部分,提到了苦境: vv.1-4是敵人的攻擊;vv.7-15是過去的家人、同伴對他的攻擊;vv.19-21是人加倍地攻擊;一次比一次強烈! B的部分是呼求: vv.5-6是自省悔改的禱告;vv.13-18求神拯救我;vv.22-28求主攻擊敵人、伸張正義;vv.29-36卻把禱告提升到讚美的層次,並且是從vv.29-31大衛的讚美,到vv.32-33謙卑人的讚美,再到vv.34-36眾人回應的讚美。當攻擊越強度,禱告的強度也越強,從認罪、呼求公義到讚美,並且讚美的廣度與強度也在增強,這是屬靈的原則,攻擊越大,讚美越大。 大衛關心的重點是聖殿(v.9),為神的殿心中焦急,當時應是聖殿中充滿了許多不應該有的狀況,而大衛選擇哭泣、禁食、披麻禱告,但是卻因著他的敬虔成為人的笑柄。 大衛一方面求主拯救、求主刑罰,二方面也在這樣的苦境中學習讚美。vv.30-31以詩歌讚美神,以感謝稱神為大,選擇獻祭給神。而且不只是自己讚美,vv.32-33期待所有謙卑人、尋求神的人都可以甦醒,起來讚美神;vv.34-36更期待所有天地萬物都要讚美神,當然也包括期待外邦人起來讚美神,這種對神殿的熱心是我們的榜樣,我們對神的家應當要有這樣的 急切與熱愛。 這篇詩篇也多處預表了耶穌:v.9為聖殿焦急,如同約2:17耶穌潔淨聖殿一般;辱罵落在身上,如同羅15:3基督不求自己的益處,讓辱罵落在身上。v.13悅納的時候,被林後6:2引用,就是基督拯救與悅納的時候;v.21苦膽、醋可以指向十架上的苦楚;v.22筵席也被羅11:9-10引用;v.25荒場也在路13:35指向耶路撒冷不悔改的情況,也被徒1:20引用,指向賣耶穌的猶大的下場;v.28生命冊,也被腓4:3、啟13:8,20:15引用,成為末日的盼望。v.28記錄,以及路10:20也提醒我們,我們的名字要在天上被記錄。大衛的受苦預表了耶穌的受苦,我們的苦難若是與神永恆的計畫連結,今日的苦就有極大的祝福與價值! 五、反省問題: 我是否正在面對強烈的攻擊、危機?危機是否點燃我的讚美? 我是否對神的家、對神的殿是否有大衛這樣的熱切熱愛?我當如何具體關心神的家? ---------------- 講員: 貴格會合一堂 徐坤靖牧師 聖經之鑰-各卷書播放清單: https://thfc.pse.is/3epsdf 【聖經之鑰 相關資源】YouTube: https://thfc.pse.is/3cfams電子書: https://thfc.pse.is/3ccluu Powered by Firstory Hosting

Lichttechnik-Podcast
Der Lichttechnik-Podcast – Folge 41 – Lampensockel im Haushalt

Lichttechnik-Podcast

Play Episode Listen Later Sep 30, 2021 22:21


Sprecher: Markus BauseweinAndreas MiesauerIntro/Outro: Inka Inhalt der Folge: Lampensockel im Haushalt Schraubsockel für Leuchtmittel: E27 E14 GU10 G9 / GU9 GX53 B15d; BC; B22; B22d GU5.3 G4 / GU4 G53 Email: Lichttechnik-podcast@gmx.de  Xing:  https://www.xing.com/profile/Andreas_Miesauer/cv https://www.xing.com/profile/Markus_Bausewein/cv Linkedin:  https://www.linkedin.com/in/andreas-miesauer https://www.linkedin.com/in/markus-bausewein

聖經之鑰
撒母耳記上-第2章-撒母耳的敬虔對比以利家的墮落【聖經之鑰】1Samuel

聖經之鑰

Play Episode Listen Later Sep 2, 2021 3:17


第2章 撒母耳的敬虔對比以利家的墮落 A1-11 撒母耳母親的讚美(敬虔) →1-2 讚美神的屬性 →3-5 神做顛覆性的工作-驕傲,狂妄,勇士,飽足,多兒女者 →6-7 神的主權 →11 撒母耳敬虔的事奉 B12-17 以利兒子的放縱-敬拜上的放縱,藐視神的祭物 A18-21 撒母耳家庭的敬虔蒙福-每年編織外袍,更新獻祭!21上帝降下新的生命 B22-25 以利對兒子的放縱!25上帝殺滅以利兒子 A26 撒母耳的蒙福(敬虔)-身量與神與人喜悅他的心都增長 B27-36 以利的審判 →27-28 回溯神設立祭司的恩典 →29 以利的關鍵問題-尊重人過於尊重神!就是不尊重神! →30-36 以利家將承受的刑罰-死亡(都死在中年,兩個兒子同時死);貧窮,不再擔祭司職分(永約斷絕) A3章 撒母耳領受關於以利家受審的信息 結構亮光:這章同時有兩個主軸,好像場上有兩場戲透過探照燈讓我們交替的來看,並且在這樣的對比中看見上帝的心意!一個主軸是撒母耳(哈拿)的敬虔-1-10哈拿的讚美;11撒母耳忠心事奉;18-21撒母耳家庭的敬虔蒙福,26撒母耳身量與神與人喜悅他的心都增長;特別在其中提到了受膏者-10(35);讓這個孩子加入神救贖的工作! 另一個對比的主軸是以利家的墮落-12-17以利孩子的放縱-搶奪獻祭者;搶奪上帝的;使用暴力;(22)淫亂把聖殿變成偶像殿(廟妓);22-25以利的放縱-不尊重神,不刑罰孩子;27-36以利的審判-相對於神所設立的祭司永約,以利家喪失這樣的約;死亡要充滿這個家(中年過世,兩子同一天死);貧窮壟罩! 鑰節:2:30因此,耶和華以色列的神說:『我曾說,你和你父家必永遠行在我面前,現在我卻說,決不容你們這樣行,因為尊重我的,我必重看他;藐視我的,他必被輕視。 神與祭司家曾經立下永約,神設立這樣的祭司來服事神,是給祭司極大的權柄,但是這裡這樣的永約被打破了,打破的原因在-人藐視神!表面看來以利仍有提醒孩子,仍有清楚的是非對錯觀,上帝刑罰是否太重?不!因為根據摩西的律法,其實以利有權柄治死兩個孩子,而當以利拒絕管教,刑罰孩子,就是讓上帝來動手,同一天兩子死在戰場上!我們對上帝的愛與尊重,要遠遠超過所有人,否則上帝刑罰臨到時,沒有人擔當得起!相反的,對神的敬畏就是對家人,身邊人最大的祝福! ---------------- 講員: 貴格會合一堂 徐坤靖牧師 聖經之鑰-各卷書播放清單: https://thfc.pse.is/3epsdf 【聖經之鑰 相關資源】 YouTube: https://thfc.pse.is/3cfams 電子書: https://thfc.pse.is/3ccluu Powered by Firstory Hosting

Rule 11 Podcast: College Football Officiating Conversations
Conversation 97: The Rule 11 Challenge

Rule 11 Podcast: College Football Officiating Conversations

Play Episode Listen Later May 30, 2021 62:57


Four contestants join us to take the Rule 11 Challenge.   Each contestant will work through a play scenario and attempt to come up with the correct results.  Its a great opportunity for all of us to review some confusing rules.  Thank you to our four contestants, Guilherme, Tremain, Kevin and Taylor, for being good sports.Quote of the Week- Pleasure in the job puts perfection in the work. - AristotlePlay Scenarios from the show1.  A 4/9 @ 50, A9 punts, the kick bounces at the B-20 and hits A1 in the back of the leg at the B-16. B1 recovers the kick at the B-10 and runs to the B-30 where he fumbles. A2 scoops and scores following B1’s fumble.  Scenario A. B3 holds during the kick at the B-35 Scenario B. B3 blocks in the back at the B-24 during B1’s runScenario C. B3 holds during A2’s run at the B-202. A 4/8 @ 50, A6 punts, B1 signals for a fair catch at the B-15. B1 let’s the ball go over his head. As the ball is over B’s GL A1 bats the ball back into the field of play. B2 then picks up the ball at the B-4 and returns the ball to the B-16 where he is tackled. Scenario A. B1 blocks A2 @ the B-18 during the kickScenario B. A1 went OOB during the kick play to avoid B23. A 2/8 B-34. A7's pass is intercepted at the B-3 by B20. His original momentum carries him into his EZ. A80 tackles B20 in the EZ by grabbing and twisting his face mask. B20 stands up and spins the ball on the ground next to A80. Scenario B:  A80 grabs and twists B20's facemask causing him to fumble the ball into the field of play.  A55 recovers at the B-4 4. A Try B-3. A9 throws a pass that is intercepted in the end zone by B0.  B0 is about to be tackled in the endzone when he pitches the ball to B22 who is standing at the B-1.  B22 runs the ball across A's goal line. During the return, B55 blocks A66 below the waist at the 50 yard line.Scenario B: B55 commits an illegal blindside block against A66 at the 50 yd and then stands over him pounding his chest.  All of this action occurs with the ball carrier at the A-20.Fight Songs - Michigan, Illinois, Penn State, Ohio StateSupport the show (https://www.patreon.com/rule11)

聖經之鑰
民數記-第35章-利未人的產業,逃城之恩【聖經之鑰】Numbers

聖經之鑰

Play Episode Listen Later Apr 11, 2021 2:52


第 35 章 利未人的產業,逃城之恩 1-8 分配給利未支派的產業,48座城,其中6座是逃城 9-15 六座逃城的安排:河東3座,河西3座 16-34 逃城的運作方式 →A16-21 故意殺人者,致死(無逃城的保護) -→B22-25 過失致死者,有逃城的保護,直到大祭司過世 -→B26-29 過失致死者,離開逃城者無保護 →A30-32 故意殺人者,不可入逃城;不可收贖價讓誤殺者在大祭司未死前先回家! →33-34 逃城律法的目的:保持聖地的聖潔,流血者必須流血來償還! 結構亮光:這章主要在處理利未人的產業,而這個產業分配的說明,重點又是放在逃城的功能與目的,利未人領受產業有一個重要的目的,就是要成為那過失犯罪者的避難所! 神為什麼要祝福我們?這個背後一定有一個重要的目的,若我們失去了祝福別人的能力,目標,這一切就失去了價值!我們所領受的每個福氣都應當起來去分享,去祝福! 仔細數算神給我們的恩典,祝福,也把每個祝福都放在主的手中,求主恩膏與賜福,主啊!讓他成為許多人的福氣! 鑰節:35:25 會眾要救這誤殺人的脫離報血仇人的手,也要使他歸入逃城。他要住在其中,直等到受聖膏的大祭司死了, 流人血者,必須用流血來償還,這是神公義的律法,而過失致死者,必須讓大祭司的流血來償還! 這是彌賽亞的預言,大祭司耶穌為我們流血,使我們得著代贖,使我們所有的過犯因著耶穌而得著赦免!這是神的憐憫! 逃城也是救恩的預表,進入救恩中,所有的過犯得赦免,得到保護與憐憫! 逃城也是今天的教會,進入教會中,認識耶穌得著救恩! 即便有逃城的恩典,但是進入恩典也有條件:必須非故意殺人!今日救恩是白白的恩典,但是要進入這樣的恩典中,也有條件,就是要真正的悔改,若是不悔改,這樣的恩典與我們無份!16-21,30-32 ---------------- 講員: 貴格會合一堂 徐坤靖牧師 聖經之鑰-各卷書播放清單: https://thfc.pse.is/3epsdf 【聖經之鑰 相關資源】 YouTube: https://thfc.pse.is/3cfams 電子書: https://thfc.pse.is/3ccluu Powered by Firstory Hosting

聖經之鑰
出埃及記-第9章-第五六七災:瘟瘡雹【聖經之鑰】Exodus

聖經之鑰

Play Episode Listen Later Apr 9, 2021 2:51


第9章 第五六七災:瘟瘡雹 1-7 第五災-牲畜瘟疫(口蹄疫) A1-5 神吩咐-瘟疫臨到埃及牲畜,保護以色列的牲畜 B6 審判來到 C7 法老心固執 8-12 第六災-人身長瘡 A8-9 神吩咐-爐灰變成生瘡 B10 摩西遵行 C11-12 法術者無法站立,法老心剛硬 13-35 第七災-冰雹 A13-21 神吩咐-神可以以瘟疫殺滅,但存留為顯明神的名,審判中仍提醒可逃避之道 B22-26 摩西遵行-以色列第沒有冰雹 C27-35 法老與摩西對話 a27-28 法老:認罪-我犯罪,百姓是邪惡的, b29-33 摩西:一出城冰雹停止,但是你仍不悔改 a34-35 法老:災難停止,越發犯罪,與臣僕硬心! 結構亮光:神在審判中做「分別」的工作,4牲畜的瘟疫不臨到以色列的;26 冰雹不降在以色列地!神在審判中仍有憐憫:19 在冰雹臨到前,先警告冰雹將臨,懼怕者得存留,藐視神的被擊殺! 9:12神使法老的心剛硬; 神使他剛硬,4:21,7:3(預言);9:12(第六災後);10:1(第八災前);10:20(第八災後);10:27(第九災後);11:10(第十災之前);14:4,8,17(出埃及後) 法老自己心剛硬:7:13(警告後);7:22(第一災後);8:15(第二災後);8:19(第三災後);8:32(第四災後);9:7(第五災後,法老心固執);9:34(第七災後) 剛硬是神的預備,也是人的選擇!人無可推諉! 鑰節:16 其實,我叫你存立,是特要向你顯我的大能,並要使我的名傳遍天下。 神可以在第一災就將惡人擊殺,但是神存留法老性命的原因,要讓人可以認識神的大能! 義人存留的目的在透過義行彰顯上帝的旨意;惡人存在的目的在透過惡行彰顯神的審判!神可以在每一種人身上得著榮耀,讓人可以認識神! 差別點在於我們要成為合主所用的義人,還是被主所用的惡人!求主憐憫! 13 耶和華對摩西說:「你清早起來,站在法老面前,對他說:『耶和華希伯來人的神這樣說,容我的百姓去,好事奉我。 14 因為這一次,我要叫一切的災殃臨到你和你臣僕,並你百姓的身上,叫你知道在普天下沒有像我的。 15 我若伸手用瘟疫攻擊你和你的百姓,你早就從地上除滅了, ---------------- 講員: 貴格會合一堂 徐坤靖牧師 聖經之鑰-各卷書播放清單: https://thfc.pse.is/3epsdf 【聖經之鑰 相關資源】 YouTube: https://thfc.pse.is/3cfams 電子書: https://thfc.pse.is/3ccluu Powered by Firstory Hosting

聖經之鑰
利未記-第20章-不聖潔者的刑罰【聖經之鑰】Leviticus

聖經之鑰

Play Episode Listen Later Apr 9, 2021 2:44


第 20 章 不聖潔者的刑罰 A1-6 宗教的罪-行將兒女給偶像者,交鬼-死刑 →B7-8 吩咐要謹守神的律例 -→C9-21 家庭的罪 --→9 咒罵父母-死刑 --→10-21 婚姻的不聖潔-死刑-鄰舍,一等親,同姓,獸,無子女 →B22-26 吩咐要謹守神的律例,不可隨從當地的風俗,食物聖潔, A27 宗教的罪-交鬼-死刑 結構亮光:本章針對違反聖潔律法18-19章者,應如何處置做了規範,經文的結構很清楚,A是宗教的罪,B是吩咐要謹守神的律例,C是家庭的罪,其中重點放在家庭的罪惡中! 宗教的罪當然非常嚴重,但是我們更多發生問題的其實是在家庭中,對父母的態度,對婚姻、情感的態度,要高度的戰兢! 鑰節:20:4 那人把兒女獻給摩洛,本地人若佯為不見,不把他治死, 這裏不單提到親自把兒女獻給偶像的罪行,也特別提到了視而不見,姑息養奸,這樣的行為要與犯罪者受同樣的刑罰,這裡不是太過度的嚴苛,而是我們對在群體中的罪惡必須有的敏感度!求主使我們要謹慎自己與自己的弟兄姐妹,到底現在屬靈的光景如何,是否有拜偶像的問題?求主憐憫! 與經期的婦女同房,似乎有不同的處理方式: 20:18 婦人有月經,若與她同房,露了她的下體,就是露了婦人的血源,婦人也露了自己的血源,二人必從民中剪除。 15:24 男人若與那女人同房,染了她的污穢,就要七天不潔淨,所躺的床也為不潔淨。 20章這裡應當不是指與經期的婦女同房,而是指長期血漏的婦女! ---------------- 講員: 貴格會合一堂 徐坤靖牧師 聖經之鑰-各卷書播放清單: https://thfc.pse.is/3epsdf 【聖經之鑰 相關資源】 YouTube: https://thfc.pse.is/3cfams 電子書: https://thfc.pse.is/3ccluu Powered by Firstory Hosting

Rule 11 Podcast: College Football Officiating Conversations
Conversation 90: Player Focus - The Snapper

Rule 11 Podcast: College Football Officiating Conversations

Play Episode Listen Later Feb 14, 2021 50:41


Tyler and Dwayne tackle their first rules discussion of Season 4; starting a series called Player Focus. In this conversation, we talk about rules regarding The Snapper. We define what makes a player the snapper and what they can and can't do. Additionally discuss why its important to not only define who the snapper is, but when a player becomes the snapper. Quote of the Week“Never let the things you want make you forget the things that you have” -UnknownMentioned on the ShowPlay Scenario A 3/10 B-32. A1 throws a pass that is intercepted in the back of the end zone by B22. As B22 is running around the trying to escape, B55 is flagged for holding at the B-14. B22 takes a knee in the endzone. B 1/10 B-7 SNAP PC :25 The result of the play is a TB. The intercepting team fouls after the change of Team possession. The basic spot is the succeeding spot (B-20). The Team in possession fouls behind the basic spot so we will enforce from the spot of the foul half the distance. [8.6.1, 9.3.3.b, 10.2.2.d.2.a]Conversation 38: Basic Spot Focus - 10.2.2.d.2 and 8.5.1 exceptionhttps://www.rule11officials.com/podcast/episode/32729054/conversation-38-basic-spot-focus-1022d2-and-851-exceptionConversation 56: Practice Tips and Numbering Exceptionshttps://www.rule11officials.com/podcast/episode/4677697c/conversation-56-practice-tips-and-numbering-exceptionsFight Song - Wisconsin Support the show (https://www.patreon.com/rule11)

J格來尬聊
EP4︱好情人挑選手冊! 記得愛愛愛愛上所有的自己 feat.見習網美小吳、聖元、Yoyo

J格來尬聊

Play Episode Listen Later Apr 9, 2020 50:00


發現另一半玩交友軟體你該怎麼辦? 男生都沒動靜女生該主動告白嗎? 見習網美小吳 、聖元、Yoyo來了!!!強勢逼近單集五十分鐘,笑到胃痛是都聊了什麼東西? 戳破那些假曖昧真渣男,教妳如何辨別成為備胎的徵兆! 交友軟體正確使用方法,網友第一次見面地點好選擇 精神出軌V.S肉體出軌,要怎麼接受還是就放生我吧? 最受不了另一半的行為,聽完後你也能做個貼心的情人 告白不一定是要個什麼結果,不留遺憾表達清楚自己的感覺才重要 來跟我們一起聊聊感情裡的J個辣個吧! 來自Dcard徵文活動 那些說不出口的感情故事,讓閻奕格幫你說! 話題(一) B58 被渣男當備胎後來遇見暖男真愛 話題(二) B68 柏拉圖式的愛:在交友網站上聊出跨國網戀 話題(三) B22 女生告白並不可恥,勇敢的表達自己心聲吧 見習網美小吳YT channel 見習網美小吳Instagram 聖元Instagram Yoyo Instagram 數位收聽"少了一件牛仔褲"EP:https://orcd.co/where_are_my_jeans Janice Yan閻奕格 Facebook: https://www.facebook.com/janiceyanyige/ Janice Yan閻奕格 Instagram: http://instagram.com/janiceyanmusic

The Behavioral Observations Podcast with Matt Cicoria
Practical Functional Assessment: Session 94 with Greg Hanley

The Behavioral Observations Podcast with Matt Cicoria

Play Episode Listen Later Sep 16, 2019 122:51


Greg Hanley - the most popular Behavioral Observations guest by quite a bit - returns to the show for a fourth time, and this may be my favorite conversation with him yet! In this episode, we talk about the following: Why he started his new consultancy, FTF Behavioral Consulting, How he arrived at the decision to leave his academic post at Western New England University, Why he chose to "re-brand" the IISCA approach - which he now refers to as Practical Functional Assessment, How the PFA model has evolved over time, What should students and practitioners make regarding the divergent views between the Standard Functional Analysis and the Practical Functional Assessment models, How the PFA model can be construed as Trauma-Informed treatment, The BALANCE parent training protocol, What it's like to have PFA referenced in the 3rd edition of Cooper, Heron, and Heward, How Greg prepares for a conference presentation (in which we reference Pat Friman's contributions), What it's like to be an "ABA Rock Star," Lastly, we take several questions from The Behavioral Observations Membership Group. In answering these questions, Greg provides valuable insight into the nuances of this assessment and treatment approach for problem behavior. Greg cites a number of studies, and instead of listing them out here, I'd rather direct you to the website he's built to disseminate this work. There, you'll find a list of publications on this topic. Here's a list of a few other resources and trainings: The Facebook group for BCBAs Using the IISCA, The training event he and his colleagues are hosting on October 5th, in Worcester, MA, A two-part workshop on the BALANCE protocol at this year's NJ Autism Society's annual conference (see events B22 and B33), OK, I couldn't help myself, here is a paper that Greg mentioned not found on his site: Van Haaren (2015). Session 93 is brought to you by: Behavior University. Behavior University provides university-quality continuing education for everyday practitioners. To learn more about their CE offerings, discounts for podcast listeners, etc... please visit behavioruniversity.com/observations. The First Annual Tate Behavioral Conference taking place on October 25th in Springfield, Massachusetts, featuring presentations by Megan Miller, Ryan O'Donnell, Yours Truly, and Kim Berens. It will also include a live recording of the Behavioral Observations Podcast with my interview with Kim Berens. Here are a few other important details: 6 BACB CE credits (including 1.5 Ethics CEs) Oh, and save 15% off of your registration by using the promo code, "Matt" Did I mention that there's a free lunch? GoLotus. Go Lotus is an intuitive and easy to use, practice management system. It handles every aspect of practice management from data collection, to scheduling and billing, and more. It is so simple your entire team can be up and running in less than an hour. Listeners who use the promo code MATT, the first 100 people will receive 90 days of our data trackers completely free. And by signing up, you’ll then receive an additional discount of 25% off the first 12 months. For more info, head over to golotus.com/register.

The Drunk Leading the Blind

Self Entitled - Travis gets a little pushy in the begining. The lads celebrate a full year of podcasting… We know, a full year of this crap. Travis thinks that his "Relationship" will last longer than the podcast. Not likely. Cody and Ed rip Travis a new one about his relationship, and how he acts. Travis wishes we had fans…. Cody goes on the defensive. We have had over 4,000 listens. They love us, they really love us… well maybe they do. Questions? Hit us up on social media, @IAmCodyL, @TSTech1216 and @blindworrell. #WhyTravisWillBeSingle… Trolling on the internet, is it for you? Developers, Developers, developers. B22. Travis talks about some pretty cool technology that has helped a completely blind guy become an independent marathon runner. And as always you can find us on. Twitter: @DLBCast, @IAmCodyL, @TSTech1216 and @blindworrell Facebook: facebook.com/thedrunkleadingtheblind --- Send in a voice message: https://anchor.fm/dlbcast/message

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 04/06
The value of fluorescence and B22 techniques as complementary approaches in protein stability analyses

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 04/06

Play Episode Listen Later Jun 1, 2011


Recombinant proteins gain more and more importance in the pharmaceutical market. One major challenge is to assure the protein integrity during manufacturing, shipping and storage. Complementary analytical approaches are deemed necessary to fully characterize and understand structural changes of the protein and the quality of formed aggregates. Hence, the focus of this thesis was the investigation of protein unfolding and protein-protein interactions as first steps of protein aggregation. Using extrinsic fluorescence analysis and B22 techniques, protein unfolding as well as attractive or repulsive protein-protein interactions could be determined for both the protein aggregates as well as the monomer, due to prior size-separation, simultaneously and individually. As both fluorescence and B22 analyses can be conducted in combination with SEC, the most widely used and best established technique, the developed methods represent easily accessible and complementary approaches to analyze protein unfolding, interactions and aggregation. In sum, this allows to shed more light on the induction factors and the sequence of events in protein aggregation during formulation development, manufacturing and storage.