Podcasts about Fluorescence

I read from fluor to fluorometer.     Fluorescence is a fun word to say.  https://en.wikipedia.org/wiki/Fluorescence     The word of the episode is "fluorocarbon".     Use my special link https://zen.ai/thedictionary to save 30% off your first month of any Zencastr paid plan.    Create your podcast today! #madeonzencastr     Theme music from Jonah Kraut https://jonahkraut.bandcamp.com/     Merchandising! https://www.teepublic.com/user/spejampar     "The Dictionary - Letter A" on YouTube   "The Dictionary - Letter B" on YouTube   "The Dictionary - Letter C" on YouTube   "The Dictionary - Letter D" on YouTube   "The Dictionary - Letter E" on YouTube   "The Dictionary - Letter F" on YouTube     Featured in a Top 10 Dictionary Podcasts list! https://blog.feedspot.com/dictionary_podcasts/     Backwards Talking on YouTube: https://www.youtube.com/playlist?list=PLmIujMwEDbgZUexyR90jaTEEVmAYcCzuq     https://linktr.ee/spejampar dictionarypod@gmail.com https://www.facebook.com/thedictionarypod/ https://www.threads.net/@dictionarypod https://twitter.com/dictionarypod https://www.instagram.com/dictionarypod/ https://www.patreon.com/spejampar https://www.tiktok.com/@spejampar 917-727-5757

We've spent the better part of the last two decades unravelling exactly how the human genome works and which specific letter changes in our DNA affect things like diabetes risk or college graduation rates. Our knowledge has advanced to the point where, if we had a safe and reliable means of modifying genes in embryos, we could literally create superbabies. Children that would live multiple decades longer than their non-engineered peers, have the raw intellectual horsepower to do Nobel prize worthy scientific research, and very rarely suffer from depression or other mental health disorders.The scientific establishment, however, seems to not have gotten the memo. If you suggest we engineer the genes of future generations to make their lives better, they will often make some frightened noises, mention “ethical issues” without ever clarifying what they mean, or abruptly change the subject. It's as if humanity invented electricity and decided [...] ---Outline:(02:17) How to make (slightly) superbabies(05:08) How to do better than embryo selection(08:52) Maximum human life expectancy(12:01) Is everything a tradeoff?(20:01) How to make an edited embryo(23:23) Sergiy Velychko and the story of super-SOX(24:51) Iterated CRISPR(26:27) Sergiy Velychko and the story of Super-SOX(28:48) What is going on?(32:06) Super-SOX(33:24) Mice from stem cells(35:05) Why does super-SOX matter?(36:37) How do we do this in humans?(38:18) What if super-SOX doesn't work?(38:51) Eggs from Stem Cells(39:31) Fluorescence-guided sperm selection(42:11) Embryo cloning(42:39) What if none of that works?(44:26) What about legal issues?(46:26) How we make this happen(50:18) Ahh yes, but what about AI?(50:54) There is currently no backup plan if we can't solve alignment(55:09) Team Human(57:53) Appendix(57:56) iPSCs were named after the iPod(58:11) On autoimmune risk variants and plagues(59:28) Two simples strategies for minimizing autoimmune risk and pandemic vulnerability(01:00:29) I don't want someone else's genes in my child(01:01:08) Could I use this technology to make a genetically enhanced clone of myself?(01:01:36) Why does super-SOX work?(01:06:14) How was the IQ grain graph generated?The original text contained 19 images which were described by AI. --- First published: February 19th, 2025 Source: https://www.lesswrong.com/posts/DfrSZaf3JC8vJdbZL/how-to-make-superbabies --- Narrated by TYPE III AUDIO. ---Images from the article:

Fluorescence by Ly

In this episode of the Back of the Napkin, we dive into the world of fluorescence imaging with Luke Lavis. Known for his pioneering work at the Janelia Research Campus, Luke shares his unique journey from the woods of Oregon to the chemistry lab. Luke's story illustrates how diverse experiences can lead to groundbreaking scientific achievements.Discover how Luke's work has pushed the boundaries of fluorescence imaging. Notably, his development of Janelia Fluor® dyes has significantly improved live-cell and super-resolution imaging capabilities. This episode reveals the intricate balance between fundamental research, application in drug discovery, and the collaborative culture at Janelia, emphasizing the importance of mentorship and interdisciplinary interactions.- Follow Luke on X: @rhodamine110- Explore the Janelia Research Campus's Open Chemistry initiative for pre-commercial compounds: dyes.janelia.org- Learn more about HHMI and the Janelia Research Campus: HHMI Janelia- Janelia Fluor(R) dyes for super-resolution microscopy available at Bio-Techne Janelia Fluor® Dyes for Super Resolution Microscopy | Bio-Techne- Follow Alex: @MoloneyAlex

In this episode, I'll discuss diamond fluorescence, a topic that is rarely talked about. I'll explain what fluorescence is in my own words, and how it affects the appearance and value of a diamond. I'll also address the question of whether fluorescence is a "good or bad" thing and per usual, how the ultimate answer to that question comes down to personal preference. You'll learn a few things about this lesser-known aspect of diamond buying. Takeaways Fluorescence refers to the discharge of light that occurs when a diamond is exposed to ultraviolet light. Approximately 25-30% of diamonds exhibit some degree of fluorescence. Fluorescence can cancel out the yellowish tint in near colorless diamonds, making them appear whiter. Fluorescence can lower the price of diamonds, making them a bargain for some buyers. Personal preference is key when deciding whether fluorescence is desirable in a diamond. If you need expert advice about something you don't know much about, and need a little guidance, send me an email at andy@buylikeaguy.com and I'll do my best to cover it in an upcoming show.  If you're “that guy or girl,” that people should hear from because you know your sh*t about a certain subject, please reach out to me, and let's talk about how we can work together. Music credits: Preacher Man by Miles Neilson and The Rusted Hearts, used with permission. A killer band with original songs that get stuck in your head.  They're awesome. Listen To Preacher Man on Spotify: https://open.spotify.com/track/7ImcaJKIk0ZVtPzuUVV4vc?si=80581c74a9be4987

Episode: 3298 Gender, Color, and Dichotomy in Tennis.  Today, we take sides in tennis.

I got the opportunity to talk to Dr. Martin Chalfie, 2008 Nobel Prize Winner in Chemistry. He is an internationally recognized scientist in green fluorescence proteins, C elegans, and touch nerve development. We also talked about his challenges of finding a career in his early days. ---------------------------------------Thanks to the sponsors:Audible: Use my link for a 30-day free trial: http://audibletrial.com/diamondgoatNewsly: https://newsly.me promo code to receive a 1-month free premium subscription: EARLYMORNINGLibysn: https://libsyn.com promo code: DGDubby Energy: https://www.dubby.gg promo code for 10% off: DIAMONDGOATSwift Grips: https://swiftgrips.net, Promo code for 10% off: DiamondgoatOpus Clips: https://www.opus.pro/?via=diamondgoat----------------------------------------------------------------------------------Listen on:Podcast website: https://anchor.fm/diamondgoatSpotify: https://open.spotify.com/show/0EuhA6WyuerHtVAqcFrFeORadioPublic: https://radiopublic.com/dg-earlymorning-show-WoML4rBreaker: https://www.breaker.audio/dg-early-morning-showPodcast YT channel clips: https://www.youtube.com/@dgearlymorningshowReason: https://reason.fm/podcast/dg-earlymorning-showApple Podcast: https://podcasts.apple.com/us/podcast/dg-early-morning-show/id1575451533Amazon Music: https://music.amazon.com/podcasts/f050b86c-1dad-4bc3-b12f-6aa5fa62438cTiktok: @dgearlymorningshow --------------------------------------Check out my other stuff:Instagram: @itzdiamondgoatTwitter: @lildiamondgoatMain YT channel: youtube.com/diamondgoatTiktok: @lildiamondgoatSoundcloud: @Lil DiamondgoatSpotify: @Lil DiamondgoatMerch store: https://diamondgoat.creator-spring.com

A few weeks ago my music friend Soundpainter (aka Andrew Janik) got in touch about using one of my tracks from my Sound Installations album as he thought that it would work well with the track that he was producing. This is the result. It has been great fun going back and forth with Andrew exchaning ideas.

Only a few sleeps to EAU 2024!. And to entertain you as you travel to Paris, here is the 2nd episode of the European Urology Oncology (EUO) podcast. Host, Dr Renu Eapen (Melbourne) dives into 2 key papers from the Feb edition of the journal, featuring interviews with the lead authors. Includes a very special EUO bio segment with EU managing editor (for almost 20 years!), Cathy Pierce. Enjoy!Priority papers:1.     First-in-human Evaluation of a Prostate-specific Membrane Antigen-targeted Near-infrared Fluorescent Small Molecule for Fluorescence-based Identification of Prostate Cancer in Patients with High-risk Prostate Cancer Undergoing Robotic-assisted Prostatectomy.https://euoncology.europeanurology.com/article/S2588-9311(23)00146-3/fulltext Featured authors – Professor Peter Carroll and Associate Professor Hao Nguyen (UCSF) 2.     Retroperitoneal Lymph Node Dissection in Clinical Stage IIA/B Metastatic Seminoma: Results of the COlogne Trial of Retroperitoneal Lymphadenectomy in Metastatic Seminoma (COTRIMS).https://euoncology.europeanurology.com/article/S2588-9311(23)00117-7/abstractFeatured authors – Professor Axel Heidenreich (Cologne, Germany) Full index to European Urology Oncology February 2024 issue https://euoncology.europeanurology.com/issue/S2588-9311(24)X0002-4 

Matthew Silva, PhD, CEO of EMIT Imaging, answers questions from a recent webinar where he highlights the role of cryo-fluorescence tomography in advancing the field of immunotherapy research.

 Gene Protein Assay(GPA): Unveiling Tumor Heterogeneity in HER2/neu Positive Breast Cancer Authors:  Dr. Tanuja Shet, Dr. Aditi Rathi    Introduction: HER2/neu gene amplification on Fluorescence in-situ hybridization (FISH) is defined as average HER2neu gene signals > 4 and HER2:CEP17 ratio of > 2, in breast cancer. However, with tumor heterogeneity interpretation of these cut-offs is challenging and some cases test as borderline. We attempted a study analyzing Gene protein assay (GPA) which combines HER2/neu immunohistochemistry (IHC), and D-DISH (dual color dual in-situ hybridization) assay to help resolve this issue.    Methods:  A total of 31 cases reported as HER2/neu amplified with intermixed tumor heterogeneity on FISH were taken for this study. The original HER2/neu count was between 4 to 6 and ratio near 2 in most cases. GPA was done using 4B5 HER2/neu IHC and Roche/Ventana D-DISH kit on Ventana Ultra machine by doing IHC first followed by D-DISH. Result:  On GPA, 3/31 cases were reported as non-amplified, and the rest were amplified. Advantage of GPA was that it helped count D-DISH as per membrane staining and identified more tumor heterogeneity in contrast to FISH in six cases. The results of the remaining cases were the same.

On a parfois l'impression que la nuit n'existe qu'à travers son antinomie avec le jour : il existe 2 mondes, que tout oppose, et qui ne cohabitent qu'à travers de courts espaces de temps, l'aube et le crépuscule.Mais nous sommes loin d'être les seuls à vivre la nuit : elle fait partie du cycle de la grande majorité des êtres vivants sur terre. Comment la nuit nous impacte-t-elle ? Comment impacte-t-elle les animaux, les plantes ?Dans l'ombre de la nuit se cachent de nombreux mystères, peut-être arriverons-nous à tous les percer... un jour ? Ce soir, au Labo des savoirs, faisons toute la lumière sur la nuit, depuis le bar Pioche près de Talensac à Nantes et entouré.e.s de nos amis de l'association des Petits Débrouillards Grand Ouest ! Ces derniers ont été les lauréats des projets “Les temps de la nuit” soutenu par la ville de Nantes.Fluorescence et phosphorescence sont aussi au programme de cette nuit avec des ateliers et des défis pour toutes et tous avec Benjamin Gutjahr, coordinateur de projets, ainsi Ophélie Boucher et Manon Souquet, animatrices aux Petits débrouillards Grand Ouest ! Une émission tapie dans le noir avec les chroniques de Lila sur l'utilisation du noir dans les œuvres de fiction au cinéma Yeltaz sur la pollution lumineuse Une émission préparée et animée par Yeltaz et Dounia, réalisée par Sophie.

A Australian Zoologist Linda Reinhold has been studying fluorescence in mammals using road kill. She speaks to Jesse.

#69 — In this special technology focus episode, Peter O'Toole is joined by experts in Fluorescence Correlation Spectroscopy (FCS), including: • Thorsten Wohland, Professor in the Department of Biological Sciences at the National University of Singapore. • Annette Bergter, Marketing Manager, Business Sector Life Sciences at ZEISS Research Microscopy Solutions.• Chris MacDonald, Researcher and Senior Lecturer in the Department of Biology at the University of York. In this episode of The Microscopists, the panel discusses why FCS data is so rich and illustrates how challenges of developing technology and instruments to make FCS more accessible are being overcome. They also compete for the best analogy—featuring car noises, living room furniture, and much more!Watch or listen to all episodes of The Microscopists: themicroscopists.bitesizebio.com

Shownotes How you can heal yourself by rewriting the story Is healing and spirituality a solo mission? The art of being in unconditional love The truth that self-love is born is in the mundane  Are you a Tantric sex witch? The older you get the sexier you will be… Blu's gateway drug into spirituality Bio Blu is a highly entertaining and engaging storyteller, activation artist, podcast host and co-founder of Florescence – a modern mystery school for women.  She's dedicated the past decade of her life to the understanding of the medicinal nature of the plant kingdom, as well as the archetypal nature of the collective unconscious, allowing her to hold the space of unconditional love and evolution for everyone she encounters. The name Blu is an acronym meaning Beauty, Love, and Unity – and it is her life's work to anchor these frequencies into Earth by creating media as medicine. Blu's genius is born out of her journey with hearing loss, which has taken her to places of deep listening within her being and gifted her access to sensory perception that lies beyond the five senses. However, her true brilliance is in her ability to keep her feet rooted in the Earth so she can bring us profound golden truths of beauty, love and unity in a way that is playful and authentic. TimeStamp 0:00 You can heal yourself by rewriting the story 0:38 Intro: This Tantric Life Podcast 4:38 Deep healing activations 11:35 I was going deaf in my 20ies 16:26 Healing yourself through rewriting the story 18:51 VITA™ Coaching Certification 20:36 Is healing and spirituality solo? 28:14 Being in unconditional love you're actually in Divine Union can be recharging  32:17  Core of where self-love is born is in the mundane  36:48 Kundalini's inside of me 43:07 I trust life 48:27 I love playing with the multifaceted divine 50:11 Can I find devotion here? 55:05 Sex Magic 1:01:58 Snacks at a play party 1:04:42 Obliss 1:06:17 What is a play party? 1:22:17 I think I'm a tantric sex witch 1:32:01 The older you get the sexier you will be 1:34:34  My gateway drug into spirituality which was the secret 1:36:53 Fluorescence, online Sisterhood & Deja Blue Podcast 1:38:35 Quick meditation 1:41:29 Conclusion === Follow Layla!  Website: www.laylamartin.com  Instagram: https://www.instagram.com/thelaylamartin/ Sign up to receive my free weekly email that allows you to slowly master the art of experiencing confidence, power, sexiness, radiance, and true love: https://laylamartin.com/join-list/  Listen on Apple Podcasts: https://podcasts.apple.com/us/podcast/this-tantric-life-with-layla-martin/id1685418994 Listen on Spotify: https://open.spotify.com/show/72iBpAwMSsTl9zLk8fHMp1?si=9f465574aa114d63  MOOD Sexy Plant Activated Supplements: https://shopmood.com/  For Men: Learn advanced sexual skills that will make you the best possible lover by unlocking your primal power AND your partner's pleasure in Men's Sexual Mastery - https://hubs.ly/Q01c_Wgx0  Obliss: The Sexual Masterclass for Women. This 6-week online course contains 24 transformative exercises and techniques. https://hubs.ly/Q01c0SWh0  TRUTH AND LOVE COACHING INTERNATIONAL, LLC – VIDEO DISCLAIMER The information contained within this video is for informational purposes only. We shall in no event be held liable to any party for any reason arising directly or indirectly for the use or interpretation of the information presented in this video. Copyright 2023, Truth and Love Coaching, LLC - All Rights Reserved.  MB01CQAPZKAF1CI MB01CBI41GZVPCE MB01Y5QNU5OEFMH

This week they discuss 2 articles 2 years apart about protein folding and implications of this success. They smoke the Lampert 1675 Edition Azul and drink the Iron Root's Saints Alley Heretic finished in Rhum casks.  They wrap up with some sci fi talk and hope we don't miss our successes like they have. ‘The game has changed.' AI triumphs at protein folding. Dec 2020 ‘The game has changed.' AI triumphs at protein folding | Science ‘The entire protein universe': AI predicts shape of nearly every known protein Now AI Can Be Used to Design New Proteins | The Scientist Magazine® (the-scientist.com)

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.03.551868v1?rss=1 Authors: Lin, Z., Zhang, X., Nandi, P., Lin, Y., Wang, L., Chu, Y., Paape, T., Yang, Y., Xiao, X., Liu, Q. Abstract: X-ray tomography and x-ray fluorescence imaging are two non-invasive imaging techniques to study cellular structures and chemical element distributions, respectively. However, correlative X-ray tomography and fluorescence imaging for the same cell has yet to be routinely realized due to challenges in sample preparation and X-ray radiation damage. Here we report an integrated experimental and computational workflow for achieving correlative multi-modality X-ray imaging of a single cell. The method consists of the preparation of radiation-resistant single-cell samples using live-cell imaging-assisted chemical fixation and freeze-drying procedures, targeting and labeling cells for correlative x-ray tomography and x-ray fluorescence measurement, and computational reconstruction of the correlative and multi-modality images. With X-ray tomography, cellular structures including the overall structure and intracellular organelles are visualized, while X-ray fluorescence imaging reveals the distribution of multiple chemical elements within the same cell. Our correlative method demonstrates the feasibility and broad applicability of using X-rays to understand cellular structures and the roles of multiple chemical elements and related proteins in signaling and other biological processes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.18.549527v1?rss=1 Authors: Farrants, H., Shuai, Y., Lemon, W. C., Hernandez, C. M., Yang, S., Patel, R., Qiao, G., Frei, M. S., Grimm, J. B., Hanson, T. L., Tomaska, F., Turner, G. C., Stringer, C., Keller, P. J., Beyene, A. G., Chen, Y., Liang, Y., Lavis, L. D., Schreiter, E. R. Abstract: Genetically encoded fluorescent calcium indicators have revolutionized neuroscience and other biological fields by allowing cellular-resolution recording of physiology during behavior. However, we currently lack bright, genetically targetable indicators in the near infrared that can be used in animals. Here, we describe WHaloCaMP, a modular chemigenetic calcium indicator built from bright dye-ligands and protein sensor domains that can be genetically targeted to specific cell populations. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan. WHaloCaMP is compatible with rhodamine dye-ligands that fluoresce from green to near-infrared, including several dye-ligands that efficiently label the central nervous system in animals. When bound to a near-infrared dye-ligand, WHaloCaMP1a is more than twice as bright as jGCaMP8s, and shows a 7x increase in fluorescence intensity and a 2.1 ns increase in fluorescence lifetime upon calcium binding. We use WHaloCaMP1a with near-infrared fluorescence emission to image Ca2+ responses in flies and mice, to perform three-color multiplexed functional imaging of hundreds of neurons and astrocytes in zebrafish larvae, and to quantitate calcium concentration using fluorescence lifetime imaging microscopy (FLIM). Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.06.547837v1?rss=1 Authors: Bagley, D. C., Morham, S. G., Kaplan, J., Ward, D. M. Abstract: Mon1a has been shown to function in the endolysosomal pathway and the secretory pathway, interact with dynein and affecting ER to Golgi traffic. Here we show that Mon1a is also required for maintenance of the Golgi apparatus. We identified the F-BAR protein FCHO2 as a Mon1a-interacting protein by both yeast two-hybrid analysis and co-immunoprecipitation. siRNA-dependent reductions in Mon1a or FCHO2 resulted in Golgi fragmentation. Membrane trafficking through the secretory apparatus in FCHO2-depleted cells was unaltered, however, reduction of FCHO2 affected the uniform distribution of Golgi enzymes necessary for carbohydrate modification. Fluorescence recovery after photobleaching analysis showed that the Golgi ministacks in Mon1a- or FCHO2-silenced cells did not exchange resident membrane proteins. The effect of FCHO2 silencing on Golgi structure was partially cell cycle-dependent and required mitosis-dependent Golgi fragmentation, whereas the effect of Mon1a-silencing on Golgi disruption was not cell cycle-dependent. mCherry-FCHO2 transiently colocalized on Golgi structures independent of Mon1a. These findings suggest that Mon1a has functions throughout the secretory pathway including interacting with dynein at the ER-Golgi interface in vesicle formation and then interacting with FCHO2 at the Golgi to generate lateral links between ministacks, thus creating Golgi ribbons. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In this week's episode of "The Conscious Code," we delve into the captivating archetype of Gate/Gene Key 15, known as the Gate of Extremes, Humanity, or Compassion. Join us as we explore the Shadow of Dullness, the Gift of Magnetism, and the Siddhi of Fluorescence, which are the energies associated with this transformative cosmic blueprint, and discover contemplative questions and affirmations to deepen your self-reflection and integration of these energies. Recap on the Energies of Gate 12. Introduction to Gate/Gene Key 15: Discover the essence of the Gate of Extremes and how it influences our lives, relationships, and spiritual evolution. Shadow Energy: Explore the shadow aspect of Gate/Gene Key 15, which is dullness. How does vanity show up in your life? In what ways does it hinder your growth and magnetism? Gift Energy: Embrace the gift of Magnetism that Gate/Gene Key 15 brings. How can you tap into your magnetic nature to bring a sense of newness to the monotony of life? Siddhi Energy: Dive into the Siddhi energy of Fluorescence, the highest Gate/Gene Key 15 expression. How can you embody the flourishing energy that allows you to see beauty on all extremes of polarity on this planet? I also provide you with some contemplative questions as well as affirmations to help you further embody the gifts of this Gate or Gene Key! Join us as we unlock the secrets of Gate/Gene Key 15, embracing the dance of the Shadow, Gift, and Siddhi. ************* Join the Self Love Revival Membership and gain the Keys to Unlocking More Intimacy with Your Soul: https://consciouslycrystal.com/slr Register for the FREE Vibes By Design Mini-Course to learn how to read your Human Design Chart and how to use Human Design and the Gene Keys to Raise Your Vibration: https://consciouslycrystal.com/optin Get your FREE Human Design Body Chart by going to this link and entering your birth time and location: https://consciouslycrystal.com Here Are the Links to Connect With Me: Instagram: https://instagram.com/iamconsciouslycrystal TikTok: https://tiktik.com/@iamconsciouslycrystal Join the Human Design and Gene Keys for Conscious Women Facebook Group: https://facebook.com/groups/hdandgk --- Send in a voice message: https://podcasters.spotify.com/pod/show/consciouslycrystal/message Support this podcast: https://podcasters.spotify.com/pod/show/consciouslycrystal/support

TRIGGER WARNING: Miscarriage, abortion, infertility (not things we've personally experienced, but these are topics that come up in this conversation!) Hiii, beautiful vortex. Today, we're talking about WOMBS! Yes, wombs. Sparked by Elle's recent time spent learning from Zach Bush MD who opened a contemplation with her Fluorescence women's circle on the womb, this is a reverent conversation on the metaphysical portal that exists within people with wombs. It serves as a reminder of our infinite potential to create magic of all kinds. We dive deep into our thoughts on birth, the full life that exists within the womb whether a human baby is actually carried to full term or lives inside of it at all, and the way the womb symbolizes our divine power to create. This launch pad lands us in tangents on the healing power of animals, releasing the need to be understood, and, unsurprisingly, a quick episode of the Introvert Chronicles as Maya & Elle compare notes on their parallel experiences as introverts. We love you

Welcome to “Analytically Speaking,” the podcast from LCGC and Spectroscopy. Here in Episode 13, podcast host Dr. Jerry Workman talks to Dr. Paul C. DeRose, who is a senior research chemist at the National Institute of Standards and Technology (NIST) and leads the NIST Biochemical Science Division's project in luminescence standards development for chemical analysis and assay validation. We spoke to Paul about his current research interests in fluorescence and luminescence spectroscopy, specifically concerning the development of fluorescence standards and methods for validation of chemical and clinical assays. Paul's research has resulted in publications in various areas of fluorescence spectroscopy and microscopy. He has developed fluorescence standard guidelines and recommendations for ASTM, IUPAC, and the US Pharmacopeia. He is also the chair of ASTM E13.01.01 sub-committee on Molecular Luminescence.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.20.537668v1?rss=1 Authors: Tatickova, M., Trebichalska, Z., Kyjovska, D., Otevrel, P., Kloudova, S., Holubcova, Z. Abstract: Egg quality is a limiting factor of female fertility and assisted reproductive technology (ART) success. Oocytes recovered from hyperstimulated ovaries often display morphological anomalies suspected to compromise their fertilization and developmental potential. Knowledge of (ab)normal oocytes intracellular organization is vital to establish reliable criteria for morphological evaluation of oocytes intended for in vitro fertilization (IVF). Here, we investigated the fine morphology of 22 dysmorphic IVF oocytes exhibiting different types of cytoplasmic irregularities, namely (1) refractile bodies, (2) centrally-located cytoplasmic granularity (CLCG), (3) smooth endoplasmic reticulum (SER) disc, and (4) vacuoles. Transmission electron microscopy (TEM) revealed the structural basis of these aberrations and indicated that the underlying cause of two of the studied morphotypes was inordinate organelle clustering. To address the mechanism required for accurate organelle positioning, we used cytoskeleton-targeting chemical compounds and performed a series of inhibition experiments involving a total of 133 human oocytes maturing in vitro. Fluorescence and electron microscopy showed that disruption of actin, not microtubules, led to the aggregation of subcellular structures resembling the morphological pattern seen in abnormal oocytes. These results imply that actin serves as a regulator of organelle distribution during human oocyte maturation. The ultrastructural analogy between dysmorphic eggs retrieved in IVF cycles and oocytes, in which actin network integrity was perturbed, suggests that dysfunction of the actin cytoskeleton might be implicated in generating common cytoplasmic aberrations. Knowledge of human oocytes inner workings and the origin of morphological abnormalities is a step forward to more objective egg quality assessment in clinical practice. SUMMARY SENTENCEUltrastructural analysis of eggs exhibiting cytoplasmic abnormalities combined with inhibition experiments indicates that dysfunction of the actin network might be involved in the development of oocyte dysmorphisms. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.03.535393v1?rss=1 Authors: Engelmann, S. A., Dunn, A. K., Tomar, A., Woods, A. L. Abstract: Significance: Two-photon microscopy is used routinely for in vivo imaging of neural and vascular structure and function in rodents with a high resolution. Image quality, however, often degrades in deeper portions of the cerebral cortex. Strategies to improve deep imaging are therefore needed. We introduce such a strategy using gates of high repetition rate ultrafast pulse trains to increase signal level. Aim: We investigate how signal generation, signal-to-noise ratio (SNR), and signal-to-background ratio (SBR) improve with pulse gating while imaging in vivo mouse cerebral vasculature. Approach: An electro-optic modulator is used with a high-power (6 W) 80 MHz repetition rate ytterbium fiber amplifier to create gates of pulses at a 1 MHz repetition rate. We first measure signal generation from a Texas Red solution in a cuvette to characterize the system with no gating and at a 50%, 25%, and 12.5% duty cycle. We then compare signal generation, SNR, and SBR when imaging Texas Red-labeled vasculature using these conditions. Results: We find up to a 6.73-fold increase in fluorescent signal from a cuvette when using a 12.5% duty cycle pulse gating excitation pattern as opposed to a constant 80 MHz pulse train. We verify similar increases for in vivo imaging to that observed in cuvette testing. For deep imaging we find pulse gating to result in a 2.95-fold increase in SNR and a 1.37-fold increase in SBR on average when imaging mouse cortical vasculature at depths ranging from 950 m to 1050 m. Conclusions: We demonstrate that a pulse gating strategy can either be used to limit heating when imaging superficial brain regions or used to increase signal generation in deep regions. These findings should encourage others to adopt similar pulse gating excitation schemes for imaging neural structure through two-photon microscopy. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.03.535397v1?rss=1 Authors: Duan, W. Abstract: Huntington disease (HD) is a neurodegenerative disorder that presents with progressive motor, mental, and cognitive impairment leading to early disability and mortality. The accumulation of mutant huntingtin protein aggregates in neurons is a pathological hallmark of HD. The glymphatic system, a brain-wide perivascular network, facilitates the exchange of interstitial fluid (ISF) and cerebrospinal fluid (CSF), supporting interstitial solute clearance including abnormal proteins from mammalian brains. In this study, we employed dynamic glucose-enhanced (DGE) MRI to measure D-glucose clearance from CSF as a tool to assess CSF clearance capacity to predict glymphatic function in a mouse model of HD. Our results demonstrate significantly diminished CSF clearance efficiency in premanifest zQ175 HD mice. The impairment of CSF clearance of D-glucose, measured by DGE MRI, worsened with disease progression. These DGE MRI findings in compromised glymphatic function in HD mice were further confirmed with fluorescence-based imaging of glymphatic CSF tracer influx, suggesting an impaired glymphatic function in premanifest stage of HD. Moreover, expression of the astroglial water channel aquaporin-4 (AQP4) in the perivascular compartment, a key mediator of glymphatic function, was significantly diminished in both HD mouse brain as well as postmortem human HD brain. Our data, acquired using a clinically translatable MRI approach, indicate a perturbed glymphatic network in the HD brain as early as in the premanifest stage. Further validation of these findings in clinical studies should provide insights into potential of glymphatic clearance as a HD biomarker and for glymphatic functioning as a disease-modifying therapeutic target for HD. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Ahsen Ustaoglu talks to Dr Nik Dekkers and Dr Jurjen Boonstra (the Netherlands) on their recent study investigating suitable imaging biomarker targets to differentiate high-grade dysplasia (HGD) and T1 colorectal cancer (T1CRC) from low-grade dysplasia (LGD) in colorectal polyps.

Surgery along with chemotherapy is the mainstay of treatment for patients with osteosarcoma. In order to achieve a cure, during surgery healthy tissue surrounding the tumour is also removed. Whilst this reduces the chances of the cancer returning, it can also result in added pain and disability for patients, impacting their quality of life in the longer-term. Advancements in surgical technology and technique offer the hope of improved outcomes for patients. Recent developments have led to the introduction of fluorescence guided surgery (FGS), a technique which harnesses the emission of light to identify a tumour's precise location and boundaries during surgical removal. This improves a surgeon's ability to successfully remove the entire tumour, which can be identified as a ‘glowing' area of tissue, decreasing the likelihood of any cancer cells remaining, while limiting the removal of too much healthy tissue. This upcoming clinical trial, led by Kenneth Rankin, a leading Consultant Orthopaedic Surgeon and bone sarcoma researcher, is looking to assess the effectiveness of a particular fluorescent dye, indocyanine green or ‘ICG', which can be given to patients safely before surgery and leads to the sarcoma tumour fluorescing green. The SarcoSIGHT trial will recruit 500 patients undergoing surgery for bone and soft tissue sarcoma, aiming to test whether the use of ICG in FGS can help to accurately identify the tumour, aid in complete removal and reduce the amount of healthy tissue removed. This presentation will include the findings to date from fluorescence guided surgery in osteosarcoma patients with some initial results indicating that the amount of fluorescence may predict response to chemotherapy and that the osteosarcoma tissue can be studied in detail post-operatively with the latest fluorescence microscopy techniques. --- Mr Kenneth Rankin is a Consultant Orthopaedic Surgeon at Newcastle's Freeman Hospital where his specialist interests are in orthopaedic oncology including fluorescence guided surgery for sarcoma resection, and hip and knee replacement for arthritis. Mr Rankin graduated in 1999 from the University of Dundee. His basic surgical training was in Newcastle followed by an MD investigating the cellular biology of bone metastases. Mr Rankin completed his higher specialist training in Perth and Dundee followed by a return to the North East as NIHR Academic Clinical Lecturer. His current post as Consultant Orthopaedic Surgeon and Honorary Senior Lecturer is comprised mainly of orthopaedic oncology including the surgical management of bone and soft tissue sarcomas and metastatic bone disease. He also carries out hip and knee replacements for arthritis. As a Clinical Scientist Mr Rankin has developed an international reputation for translational research for the detection of circulating tumour cells in sarcoma patients and carried out the world's first case series of fluorescence guided surgery in sarcoma. Working in close collaboration with scientists at Newcastle University, he leads on basic and translational sarcoma research at the Newcastle Centre for Cancer.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.28.530395v1?rss=1 Authors: Marulanda-Gomez, A. M., Bayer, K., Pita, L., Hentschel, U. Abstract: Sponges harbor diverse, specific, and stable microbial communities, but at the same time, they efficiently feed on microbes from the surrounding water column. This filter-feeding lifestyle poses the need to distinguish between three categories of bacteria: food to digest, symbionts to incorporate, and pathogens to eliminate. How sponges discriminate between these categories is still largely unknown. Phagocytosis is conceivable as the cellular mechanism taking part in such discrimination, but experimental evidence is missing. We developed a quantitative in-vivo phagocytosis assay using an emerging experimental model, the sponge Halichondria panicea. We incubated whole sponge individuals with different particles, recovered the sponge (host) cells, and tracked the particles into the sponge cells to quantify the sponge phagocytic activity. Fluorescence-activated cell sorting (FACS) and fluorescent microscopy were used to quantify and verify phagocytic activity (i.e., the population of sponge cells with internalized particles). Sponges were incubated with a green microalgae to test the effect of particle concentration on the percentage of phagocytic activity, and to determine the timing where the maximum of phagocytic cells are captured in a pulse-chase experiment. Lastly, we investigated the application of our phagocytic assay with other particle types (i.e., bacteria and fluorescent beads). The percentage of phagocytic cells that had incorporated algae, bacteria, and beads ranged between 5 to 24 %. We observed that the population of phagocytic sponge cell exhibited different morphologies and sizes depending on the type of particle presented to the sponge. Phagocytosis was positively related to algal concentration suggesting that sponge cells adjust their phagocytic activity depending on the number of particles they encounter. Our results further revealed that sponge phagocytosis initiates within minutes after exposure to the particles. Fluorescent and TEM microscopy rectified algal internalization and potential digestion in sponge cells, and suggests translocation between choanocyte and archeocyte-like cells over time. To our knowledge, this is the first quantitative in-vivo phagocytosis assay established in sponges that could be used to further explore phagocytosis as a cellular mechanism for sponges to differentiate between different microorganisms. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.15.528510v1?rss=1 Authors: Gates, S. J., Alvarez, P. H., O'Neill, K. M., Cao, K., Losert, W. Abstract: : Waves and oscillations play a key role in the flow and processing of information in the brain. Recent work has demonstrated that in addition to electrical activity, biomechanical signaling can also be excitable and thus capable of self-sustaining oscillations and waves. Here we measured the biomechanical dynamics of actin polymerization in neural precursor cells throughout their differentiation into populations of neurons and astrocytes. Fluorescence-based live-cell imaging allowed us to analyze the dynamics of actin in conjunction with the dynamics of calcium signals. Actin dynamics throughout differentiation showed a rhythmic character, localized mostly in processes, with changes in scale associated with differentiation. Furthermore, actin dynamics impact ionic dynamics, with an increase in the frequency of calcium bursts accompanied by a decrease in cell-cell correlations when actin dynamics is inhibited. This impact of cytoskeletal dynamics on cell-cell coupling and ionic neural cell signaling suggests that information flow in the brain may be able to harness both biomechanical and electrical/ionic excitability. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

GUEST: Zaver Bhujwalla, BaltimoreIn this episode we discuss how targeting fibroblasts rather than tumour cells may be an effective strategy for both surgical guided resection and as an anti-cancer therapy with Tim's “academic grandmother”, Zaver Bhujwalla. We discovered Zaver's roots as a physicist and mathematician, learned about her (unpredictable) interest in cancer metabolism and how she combines tools to develop a powerful in vitro diagnostic test.Selected Publication: "Design and characterization of fibroblast activation protein targeted pan-cancer imaging agent for fluorescence-guided surgery of solid tumors" published in Journal of Materials Chemistry B, by the the authors Mukkamala R, Lindeman SD, Kragness KA, Shahriar I, Srinivasarao M, Low PS (Purdue University, West Lafayette, USA).Publication reference: Mukkamala R, Lindeman SD, Kragness KA, Shahriar I, Srinivasarao M, Low PS. Design and characterization of fibroblast activation protein targeted pan-cancer imaging agent for fluorescence-guided surgery of solid tumors. J Mater Chem B. 2022 Mar 23;10(12):2038-2046. doi: 10.1039/d1tb02651h.https://pubs.rsc.org/en/content/articlelanding/2022/TB/D1TB02651HFurther information on the European Society for Molecular Imaging:https://e-smi.eu/Contact: office@e-smi.eu

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.28.526043v1?rss=1 Authors: Dvinskikh, L., Sparks, H., Brito, L., MacLeod, K. T., Harding, S. E., Dunsby, C. Abstract: Improving cardiac function through stem-cell regenerative therapy requires functional and structural integration of the transplanted cells with the host tissue. Visualizing the electromechanical interaction between native and graft cells necessitates 3D imaging with high spatio-temporal resolution and low photo-toxicity. A custom light-sheet fluorescence microscope was used for volumetric imaging of calcium dynamics in co-cultures of adult rat left ventricle cardiomyocytes and human induced pluripotent stem cell-derived cardiomyocytes. Aberration-free remote refocus of the detection plane synchronously to the scanning of the light sheet along the detection axis enabled fast dual-channel 3D imaging at subcellular resolution without mechanical sample disturbance at up to 8 Hz over a {approx}300 m x 40 m x 50 m volume. The two cell types were found to undergo electrically stimulated and spontaneous synchronized calcium transients and contraction. Electromechanical coupling was found to improve with co-culture duration, with 50% of adult-CM coupled after 24 hours of co-culture, compared to 19% after 4 hours (p = 0.0305). Immobilization with para nitroblebbistatin did not prevent calcium transient synchronization, with 35% and 36% adult-CM coupled in control and treated samples respectively (p = 0.91), indicating that electrical coupling can be maintained independently of mechanotransduction. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

The Speaking of Wounds podcast series is creating by the Wound Care Learning Network.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.14.520430v1?rss=1 Authors: Morrow, C. S., Tweed, K., Arndt, Z. P., Walsh, A. J., Peng, B., Risgaard, R. D., Klosa, P. C., Chi, M. M., Wallace, E. P., Jones, M. V., Roopra, A., Skala, M. C., Moore, D. L. Abstract: Neural stem cells (NSCs) in the adult brain are primarily quiescent but can activate and enter the cell cycle to produce newborn neurons. NSC quiescence can be regulated by disease, injury, and age, however our understanding of NSC quiescence is limited by technical limitations imposed by the bias of markers used to isolate each population of NSCs and the lack of live-cell labeling strategies. Fluorescence lifetime imaging (FLIM) of autofluorescent metabolic cofactors has previously been used in other cell types to study shifts in cell states driven by metabolic remodeling that change the optical properties of these endogenous fluorophores. Here we asked whether autofluorescence could be used to discriminate NSC activation state. We found that quiescent NSCs (qNSCs) and activated NSCs (aNSCs) each have unique autofluorescence intensity and fluorescence lifetime profiles. Additionally, qNSCs specifically display an enrichment of a specific autofluorescent signal localizing to lysosomes that is highly predictive of cell state. These signals can be used as a graded marker of NSC quiescence to predict cell behavior and track the dynamics of quiescence exit at single cell resolution in vitro and in vivo. Through coupling autofluorescence imaging with single-cell RNA sequencing in vitro and in vivo, we provide a high-resolution resource revealing transcriptional features linked to rapid NSC activation and deep quiescence. Taken together, we describe a single-cell resolution, non-destructive, live-cell, label-free strategy for measuring NSC activation state in vitro and in vivo and use this tool to expand our understanding of adult neurogenesis. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Before x-rays were used for medical purposes, they were used for entertainment. X-rays are part of the electromagnetic spectrum and are utilized in a variety of radiography procedures that are currently taught to aspiring veterinary students. Liane Shaw, Diagnostic Imaging Senior Instructional Veterinary Nurse at the Purdue University College of Veterinary Medicine, describes the use of x-rays within these procedures and gives us a crash course in the history and properties of x-ray waves.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.23.517628v1?rss=1 Authors: Zhang, Q., Yang, Y., Cao, K. J., Chen, W., Paidi, S., Xia, C.-h., Kramer, R., Gong, X., Ji, N. Abstract: The retina, behind the transparent optics of the eye, is the only neural tissue whose physiology and pathology can be non-invasively probed by optical microscopy. The aberrations intrinsic to the mouse eye, however, prevent high-resolution investigation of retinal structure and function in vivo. Optimizing the design of a two-photon fluorescence microscope (2PFM) and sample preparation procedure, we found that adaptive optics (AO), by measuring and correcting ocular aberrations, is essential for resolving synapses and achieving three-dimensional cellular resolution in the mouse retina in vivo. Applying AO-2PFM to longitudinal retinal imaging in transgenic models of retinal pathology, we characterized microvascular lesions and observed microglial migration in a proliferative vascular retinopathy model, and found Lidocaine to effectively suppress retinal ganglion cell hyperactivity in a retinal degeneration model. Tracking structural and functional changes at high resolution longitudinally, AO-2PFM enables microscopic investigations of retinal pathology and pharmacology for disease diagnosis and treatment in vivo. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.11.14.516420v1?rss=1 Authors: Willekens, S. M., Morini, F., Mediavilla, T., Nilsson, E., Oradd, G., Hahn, M., Chotiwan, N., Visa Majoral, M., Berggren, P.-O., Ilegems, E., Overby, A. K., Ahlgren, U., Marcellino, D. Abstract: Optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) are high resolution optical imaging techniques operating in the mm-cm range, ideally suited for ex vivo 3D whole mouse brain imaging. Although these techniques exhibit high sensitivity and specificity for antibody labeled targets, the provided anatomical information remains limited. To allow anatomical mapping of fluorescent signal in whole brain, we developed a novel magnetic resonance (MR) based template with its associated tissue priors and atlas labels, specifically designed for brains subjected to tissue processing protocols required for 3D optical imaging. We investigated the effect of tissue preprocessing and clearing on brain size and morphology and developed optimized templates for BABB/Murrays clear (OCUM) and DBE/iDISCO (iOCUM) cleared brains. By creating optical (i)OCUM fusion images using our mapping procedure, we localized dopamine transporter and translocator protein expression and tracer innervation from the eye to the lateral geniculate nucleus of thalamus and superior colliculus. These fusion images allowed for precise anatomical identification of fluorescent signal in discrete brain areas. As such, these templates enable applications in a broad range of research areas integrating optical 3D brain imaging by providing an MR template for cleared brains. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.18.512786v1?rss=1 Authors: Romero Bhathal, J., Faisal, S., Levitt, M. R., Geindreau, C., Aliseda, A. Abstract: Biofidelic numerical models have been developed such as the coil-resolved model to study hemodynamics in the treated aneurysm. In this model, the geometry of the coils is recreated from high-resolution tomography scans of a phantom aneurysm treated with coils. However, this model hasn't been validated. The purpose of this work is to validate the coil-resolved model. To achieve this, we used the planar-laser induced fluorescence technique on phantom aneurysm treated with coils and measured the residence time and the evolution of rhodamine concentration during the washout. We run passive scalar simulations with the coil-resolved model and measured the evolution of concentration over time. The comparison of the numerical and the experimental results shows that the coil-resolved model reproduces the hemodynamics of the experimental setup. Therefore it can be used as a reference to study hemodynamics in the treated aneurysm or to validate porous media models developed for treatment outcomes prediction. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.10.20.512984v1?rss=1 Authors: Leiwe, M. N., Fujimoto, S., Baba, T., Moriyasu, D., Sakaguchi, R., Saha, B., Inagaki, S., Imai, T. Abstract: Fluorescence imaging is widely used for the mesoscopic mapping of neuronal connectivity. However, neurite reconstruction is challenging, especially when neurons are densely labelled. Here we report a strategy for the fully automated reconstruction of densely labelled neuronal circuits. Firstly, we established stochastic super-multicolour labelling with up to seven different fluorescent proteins using the Tetbow method. With this method, each neuron was labelled with a unique combination of fluorescent proteins, which were then imaged and separated by linear unmixing. We also established an automated neurite reconstruction pipeline based on the quantitative analysis of multiple dyes (QDyeFinder). To classify colour combinations, we used a newly developed unsupervised clustering algorithm, dCrawler, in which data points in multi-dimensional space were clustered based on a given threshold distance. Our new strategy allows for the reconstruction of neurites for up to hundreds of neurons at a millimetre scale without manual tracing. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.09.28.510014v1?rss=1 Authors: Ma, P., Chen, P., Tilden, E., Aggarwal, S., Oldenborg, A., Chen, Y. Abstract: Optical sensors have transformed the field of neuromodulation because neuromodulator dynamics are essential for their function. Despite their high spatial and temporal resolution, these fluorescence intensity-based sensors are sensitive to sensor expression level and excitation light fluctuation, thus preventing analysis of neuromodulators across time or animals. Here, we screened neuromodulator sensors and discovered that multiple sensors showed response in fluorescence lifetime, a property independent of sensor expression or excitation light power. The acetylcholine sensor GRAB-ACh3.0 showed the largest lifetime change. Fluorescence lifetime of GRAB-ACh3.0 responds to transient ACh release, is dose sensitive, and is insensitive to excitation laser power. In mice across sleep/wake and running/resting states, fluorescence lifetime, in contrast to intensity, predicts behavior states accurately despite change in sensor expression level across weeks and animals. Thus, fluorescence lifetime of neuromodulator sensors enables comparison of neuromodulator dynamics at high resolution across different animals, brain regions, disease models, and chronic time scales. Copy rights belong to original authors. Visit the link for more info Podcast created by PaperPlayer

Measuring interacting binary mass functions with X-ray fluorescence by Cordelia Dashwood Brown et al. on Wednesday 21 September The masses of compact objects in X-ray binaries are best constrained through dynamical measurements, relying on radial velocity curves of the companion star. In anticipation of upcoming high X-ray spectral resolution telescopes, we explore their potential to constrain the mass function of the compact object. Fe K line fluorescence is a common feature in the spectra of luminous X-ray binaries, with a Doppler-broadened component from the inner accretion disc extensively studied. If a corresponding narrow line from the X-ray irradiated companion can be isolated, this provides am opportunity to further constrain the binary system properties. Here, we model binary geometry to determine the companion star's solid angle, and deduce the iron line's equivalent width. We find that for systems with a mass ratio $q > 0.1$, the expected K${alpha}$ equivalent width is 2-40 eV. Simulations using XSPEC indicate that new microcalorimeters will have sufficient resolution to be able to produce K${alpha}$ emission line radial velocity measurements with precision of 5-40 km s$^{-1}$, for source continuum fluxes exceeding $10^{-12}$ erg cm$^{-2}$ s$^{-1}$. Several caveats need to be considered; this method is dependent on successful isolation of the narrow line from the broad component, and the observation of clear changes in velocity independent of scatter arising from complex wind and disc behaviour. These issues remain to be proven with microcalorimeters, but this method has the potential to constrain binary parameters where optical measurements are not viable. arXiv: http://arxiv.org/abs/http://arxiv.org/abs/2209.09920v1

Deducing the Composition of Venus Cloud Particles with the Autofluorescence Nephelometer AFN by Darrel Baumgardner et al. on Tuesday 06 September The composition, sizes and shapes of particles in the clouds of Venus have previously been studied with a variety of in situ and remote sensor measurements. A number of major questions remain unresolved, however, motivating the development of an exploratory mission that will drop a small probe, instrumented with a single-particle autofluorescence nephelometer (AFN), into Venus' atmosphere. The AFN is specifically designed to address uncertainties associated with the asphericity and complex refractive indices of cloud particles. The AFN projects a collimated, focused, linearly polarized, 440 nm wavelength laser beam through a window of the capsule into the airstream and measures the polarized components of some of the light that is scattered by individual particles that pass through the laser beam. The AFN also measures fluorescence from those particles that contain material that fluoresce when excited at a wavelength of 440 nm and emit at 470-520 nm. Fluorescence is expected from some organic molecules if present in the particles. AFN measurements during probe passage through the Venus clouds are intended to provide constraints on particle number concentration, size, shape, and composition. Hypothesized organics, if present in Venus aerosols, may be detected by the AFN as a precursor to precise identification via future missions. The AFN has been chosen as the primary science instrument for the upcoming Rocket Lab mission to Venus, to search for organic molecules in the cloud particles and constrain the particle composition. arXiv: http://arxiv.org/abs/http://arxiv.org/abs/2209.02054v1

When you look at a leaf, what color do you see? While a leaf may appear green, it is not necessarily producing green light. The leaf absorbs wavelengths of red light, and reflects the green light that we see. Professor Alexander Wei's research group synthesizes molecules and nanomaterials, and studies how these materials interact with light. Wei discusses luminescence, fluorescence, phosphorescence, and where we can observe these light-related properties in our everyday life. Wei's group is currently focused on designing and developing new materials that produce blue light. There is a current need for blue-light producing materials that can be used with an exciting new technology related to the next generation of flat panel television displays: Organic Light-Emitting Diodes (OLEDs).     Special thanks for support from NSF grant CHE-2204206

#11 — Fluorescence is undoubtedly one of the most important and useful tools in a biologist's toolbox. But do you actually know how fluorescence works? In this episode, discover what the three steps of fluorescence are, and how fluorescence can be used in flow cytometry. Read the full article for a breakdown of the key points of fluorescence. [1] Resources: 1. https://bitesizebio.com/32973/fluorescent-molecules/

Onze cellen hebben een slimme manier om benodigdheden als voedingsstoffen de cel in te krijgen, maar onderzoekers waren er nog niet helemaal uit hoe dat vervoer precies ontstaat. Nu weten ze weer wat meer.  Cellen hebben een membraan, een buitenste laag waar niet zomaar alles doorheen komt. Als voedingsstoffen van buiten de cel naar binnen moeten, dan gebeurt er iets heel handigs: speciale eiwitten verzamelen zich aan de binnenkant van het membraan, ze vervormen het zo dat er een soort trechter naar binnen ontstaat, daar worden de stoffen ingeladen en dan wordt de trechter een voertuigje waarmee de lading binnen in de cel wordt verplaatst.   In cellen in het lab zagen ze dit wel honderden keren per minuut gebeuren. Maar hoe die voertuigjes zich nou precies vormen, daar waren de meningen over verdeeld. Dus kregen die voertuig-eiwitjes een kleurtje – ik doe alsof het simpel is, maar hier was super high tech apparatuur voor nodig – en zo zagen ze dat er meerdere manieren zijn waarop de trechter zich kan vormen.   De vervorming van het membraan kan namelijk in gang worden gezet: nog voordat de eiwitten arriveren, vlak erna, of pas na 4 seconden. Ze denken dat onder andere het type lading een rol speelt bij de keuze tussen deze processen, maar moeten dit nog verder onderzoeken. Hoe dan ook is het weer een mooi en belangrijkje inkijkje in de werking van onze cellen, want hoe meer we weten, hoe beter we problemen in dit soort processen kunnen oplossen. Lees meer: Fluorescence microscopy shows how living cells form vesicles to transport cargo like growth factors.   See omnystudio.com/listener for privacy information.

Richardo Strausso „aljansas“, suburtas latvio Andrio Nelsono, Daliaus Naujokaičio ir Kenny Wollesseno naujausias darbas „Nojo Hotel“, šiuolaikinis Izraelio džiazas pagal leidyklą ECM, lietuvių „Vokalinio meno tinklas“ ir jų požiūris į senąjį folklorą ir ambient, šiuolaikinės muzikos ansamblis „Fluorescence“ ir... tai dar ne viskas. Laidoje ketvirtadieniais aš, Domantas Razauskas, apžvelgiu, pristatau, pasakoju apie naujausius įvairiausių tautų ir regionų džiazo, klasikos ir paralelinių žanrų visatų albumus.Ved. Domantas Razauskas

Fluorescence-guided surgery by AORNJournal

Visit https://thermofisher.com/bctl to register for your free Bringing Chemistry to Life T-shirt and https://www.alfa.com/en/chemistry-podcasts/ to access our episode summary sheet, which contains links to recent publications and additional content recommendations for our guest.Sometimes you feel like you missed an opportunity, or didn't make the best out of it, or sometimes you feel like life is unfair and doesn't offer any attractive chance. Then you hear stories like Mireille Kamariza's and your perspective changes.This is classic Bringing Chemistry to Life episode, where an incredible personal story is intertwined with great science. Dr. Mireille Kamariza, junior fellow at the Society of Fellows at Harvard University, is driven by her personal experience growing up in war-torn Burundi. She was given the opportunity to move to and study in the U.S., rose to the challenge becoming an expert in biorthogonal chemistry and developed a technology for a highly reliable, yet simple and affordable, detection method for tuberculosis. Now Mireille, nominated as one of Fortune Magazine's most powerful women, wants to give back and aims at addressing the TB global health crisis thanks to her technology.While listening to Mireille's personal story alone is well worth your time, make no mistake, there is great chemistry here. Another brilliant example of chemistry at the interface with biology, where some of the most exciting results in modern science come from.

This special two-episode edition takes a deep dive into the science behind InnerPlant with co-founder and chief science officer Rod Kumimoto and head of detection technologies, Ari Kornfeld. Episode 1 focuses on the plant biology behind the fluorescent signals that InnerPlant's crops give off when they're under stress from pathogens or a lack of water or nutrients. Episode 2 focuses on the science involved in detecting InnerPlant's optical signals in daylight from as far away as space.

This special two-episode edition takes a deep dive into the science behind InnerPlant with co-founder and chief science officer Rod Kumimoto and head of detection technologies, Ari Kornfeld. Episode 1 focuses on the plant biology behind the fluorescent signals that InnerPlant's crops give off when they're under stress from pathogens or a lack of water or nutrients. Episode 2 focuses on the science involved in detecting InnerPlant's optical signals in daylight from as far away as space.