Podcasts about psba

This week at NSTA: The Bus Stop - Executive Director Curt Macysyn is joined by  Gerry Wosewick, Executive Director of the Pennsylvania School Bus Association. Gerry and Curt delve into the Under the Hood Exemption and address some common misconceptions. The duo discuss the recent transition in the PSBA Presidency. Gerry highlights the upcoming legislative session in the Commonwealth of Pennsylvania and the items PSBA will be monitoring. Lastly, the duo preview the upcoming Baseball season. Become a subscriber and listen to a new episode of NSTA: The Bus Stop every week - targeted advertising packages are available too!Support the show

This week at NSTA: The Bus Stop - Executive Director Curt Macysyn is joined by Gerry Wosewick, Executive Director of the Pennsylvania School Bus Association. Gerry and Curt discuss several of the ongoing advocacy initiatives that PSBA is currently working on in the Commonwealth of Pennsylvania. Gerry and Curt delve into the Under the Hood Exemption. Gerry outlines the state funding formula and how PSBA is working with legislators on it. Lastly, Curt and Gerry highlight the recent 2024 PSBA Annual Convention that was held at the end of June.  Become a subscriber and listen to a new episode of NSTA: The Bus Stop every week - targeted advertising packages are available too!Support the Show.

This week at NSTA: The Bus Stop -  NSTA Executive Director Curt Macysyn interviews Gerry Wosewick, Executive Director of the Pennsylvania School Bus Association (PSBA). Gerry outlines his background and his journey to PSBA. Gerry highlights the recent PSBA Annual Convention, which took place in State College. The duo discuss PSBA legislative initiatives.  Become a subscriber and listen to a new episode every week - reasonable advertising packages are available too!Support the show

This week at NSTA: The Bus Stop NSTA Executive Director, Curt Macysyn interviews Shawn McGlinchey, Vice President of Risk Management, Krapf School Bus and President of the Pennsylvania School Bus Association, as part of the August Driver Shortage Series. Shawn outlines his background and his journey from the insurance industry to school transportation, as well as his role at Krapf School Bus, and President of the Pennsylvania School Bus Association (PSBA). Shawn outlines the driver shortage situation in the state of Pennsylvania. Curt and Shawn then discuss the study conducted by the State of Pennsylvania on the driver shortage in the state. Shawn outlines the impact of Entry Level Driver Training had on the state of Pennsylvania, and how PSBA has been able to work with state officials to help alleviate the impact of the program on contractors. Plenty of information to go along with your morning coffee at NSTA: The Bus Stop.  Support the show

On this episode of Keystone Education Radio, hear from PSBA's president-elect and Parkland School District's board president David Hein on his district's drive to continuously learn, grow and communicate effectively, and what he calls failing forward.

This week at NSTA: The Bus Stop - Ryan Dellinger, Executive Director of the Pennsylvania School Bus Association (PSBA), joins NSTA Executive Director, Curt Macysyn to discuss the current bus driver shortage affecting his state. Ryan provides a look into his background, and transition as the executive director of PSBA . Then Curt and Ryan talk about the longstanding relationship between NSTA and PSBA, and how that relationship benefits members of both organizations. Ryan highlights the current driver shortage situation in the state of Pennsylvania, and outlines the ongoing PSBA Driver Recruitment Campaign. Finally, the duo discuss the introduction of PA HR 15, which is a resolution that will analyze the driver shortage situation in Pennsylvania, and how it can potentially provide assistance in recruitment. What you need to know when you need to know it, always at NSTA: The Bus Stop. Subscribe today!Support the show (http://www.yellowbuses.org/membership/)

Meet the leaders behind PSBA’s Charter School Task Force and the Keystone Center for Charter Change at PSBA in this episode of Keystone Education Radio.

At NSTA: The Bus Stop this week, Executive Director Curt Macysyn welcomes Pennsylvania School Bus Association (PSBA) Executive Director, Mike Berk, to the podcast, where they discuss best practices when engaging your legislators.  Curt and Mike also take a deep dive into the advocacy efforts that PSBA has been undertaking that assist contractors in efforts to receive continuing payments, as well as other unique challenges cropping up in Pennsylvania that are relatable across the country. Mike describes the success the association has had meeting with legislators via Zoom calls, and strategic planning of the organization for when the legislature reconvenes. Advertise your product or service to our growing audience, more than 300 industry professionals have now subscribed to NSTA: The Bus Stop; advertising rates can be found HERE.Support the show (http://www.yellowbuses.org/membership/)

In this episode of Keystone Education Radio, podcast host Annette Stevenson is joined by Stuart Knade, PSBA’s senior director of legal services. Annette and Stuart discuss the role of collective bargaining in Pennsylvania’s public schools, including common misconceptions and the specific challenges schools face during the process.

On this episode of Keystone Education Radio, Annette Stevenson is joined by Dr. Heather Bennett, PSBA’s director of school equity services, to discuss the existing equity issues that the pandemic has further brought to light.

Join NSTA Executive Director, Curt Macysyn, as he sits down with the Pennsylvania School Bus Association (PSBA) Executive Director, Mike Berk. Learn about how PSBA unique cause, the ERIC Project, which seeks to assist special needs students in emergency scenarios. Tune in to hear more about the 2020 Bus-In and the 2020 Annual Meeting and Convention and 2020 School Bus Driver International Safety Competition. Support the show

During this episode, Heather Masshardt, director of policy services, discusses the Commonwealth Education Blueprint with David Hutchinson, PSBA’s 2019 president, and Mike Faccinetto, PSBA’s immediate past president.

In this podcast, with chatting with AHRI PhD candidate Huan Lu about his paper “Metribuzin resistance in a wild radish population via both psbA mutation and enhanced metabolism”. The authors of this paper are Huan Lu, Qin Yu, Heping Han, Mechelle J. Owen, and Stephen B. Powles. This paper identifies multiple mechanisms: as well as psbA gene mutation there is a non-target site resistance mechanism of enhanced metabolism. In his current studies, Huan Lu is documenting that this non-target site enhanced metabolism resistance endows resistance to other very different herbicides. This information will become available in Huan’s next publication. You can read the AHRI insight on this work here: https://ahri.uwa.edu.au/is-there-an-invisible-gorilla-in-your-paddock/

PSBA CEO and podcast host Nathan G. Mains talks about equity with Dr. Heather Bennett Esq., PSBA's director of Equity Services. Dr. Bennett clarifies the difference between equality and equity, and talks about Pennsylvania schools most urgent equity needs.

In the latest episode, PSBA’s Nathan Mains talks with Andy Christ, PSBA’s education policy analyst – the gentleman behind the data presented in the recently released "State of Education" report.

In this episode, PSBA’s Nathan Mains talks with PIAA Executive Director Robert Lombardi about changes in athletics, including new league classifications that contributed to near-record attendance at this year’s basketball tournament, as well as new and potential changes impacting student-athlete health and safety.

Genetically modified plants for the use of transgene containment are a central concern. Nuclear gene flow is one of the most discussed topics in our days; therefore, plastid genetic engineering is a promising tool to reduce the risk of transgene flow, because in most angiosperm species plastids are inherited maternally. In addition, plastid transformation has the advantage that the site of gene insertion can be controlled, high rates of transgene expression and protein accumulation can be achieved and epigenetic effects are absent. In Arabidopsis pollen, plastids are inherited also maternally and not created de novo, but arise from pre-existing plastids by fission. The aim of this study was to assess the frequency of plastid transfer from atrazin-resistant ElyF3BC4 Arabidopsis thaliana plants bearing a point mutation in the plastid psbA gene to male sterile N75 plants by spontaneous crossing under field conditions. Also the plastid transfer from atrazin-resistant, EMS-mutagenized M2ElyF3BC4 plants to wild-type A. thaliana plants by manual crossings under green house conditions was estimated. It was found that plastid-encoded atrazin resistance could not be transmitted via pollen, neither by manual pollination among 65,000 hybrid seeds nor by spontaneous pollination among 2,444,465 hybrid seeds in A. thaliana. Although various random nuclear mutations were screened for their potential to allow the transfer of paternal plastids into the egg-cells of the recipient plant, a corresponding mutant line could not be isolated. Explanation for this could be duplication or redundancy of nuclear genes mediating maternal inheritance and suppressing paternal leakage in Arabidopsis in such a way that the defect in one gene is compensated for by the function of its homologue. Therefore, a double mutant of two genes, atg4a and atg4b, which are involved in autophagy, were studied to test this hypothesis. However, the frequency of paternal plastid transfer was not increased. Taken together, in this study paternal leakage of Arabidopsis plastids could not be induced by mutations. To be able to follow plastid fate in developing pollen tubes, the colorless plastids in Arabidopsis pollen were visualized by the expression of a GFP fusion protein under the control of a pollen specific promoter. However, the affiliation of the GFP labeled plastids to either the vegetative or the generative cells was not clear. Placing particular emphasis on plastid behavior during specification of sperm cells in pollen of Arabidopsis might shed some light on this very strict process of maternal inheritance in the future work.

Chloroplast gene expression is predominantly regulated at the posttranscriptional levels of mRNA stability and translation efficiency. The expression of psbA, an important photosynthesis-related chloroplast gene, has been revealed to be regulated via its 5’- untranslated region (UTR). Some cis-acting elements within this 5’UTR and the correlated trans-acting factors have been defined in Chlamydomonas. However, no in vivo evidence with respect to the cis-acting elements of the psbA 5’UTR has been so far achieved in higher plants such as tobacco. To attempt this, we generated a series of mutants of the tobacco psbA 5’UTR by base alterations and sequence deletions, with special regard to the stem-loop structure and the putative target sites for ribosome association and binding of nuclear regulatory factors. In addition, a versatile plastid transformation vector pKCZ with an insertion site in the inverted repeat region of the plastid genome was constructed. In all constructs, the psbA 5’UTR (Wt or modified) was used as the 5’ leader of the reporter gene uidA under control of the same promoter, Prrn, the promoter of the rRNA operon. Through biolistic DNA delivery to tobacco chloroplasts, transplastomic plants were obtained. DNA and RNA analyses of these transplastomic plants demonstrated that the transgenes aadA and uidA had been correctly integrated into the plastome at the insertion site, and transcribed in discrete sizes. Quantitative assays were also done to determine the proportion of intact transplastome, the uidA mRNA level, Gus activity, and uidA translation efficiency. The main results are the following: 1) The insertion site at the unique MunI between two tRNA genes (trnR-ACG and trnNGUU) is functional. Vector pKCZ has a large flexibility for further DNA manipulations and hence is useful for future applications. 2) The stem-loop of the psbA 5’UTR is required for mRNA stabilisation and translation. All mutants related to this region showed a 2~3 fold decrease in mRNA stability and a 1.5~6 fold reduction in translation efficiency. The function of this stem-loop depends on its correct sequence and secondary conformation. 3) the AU-box of the psbA 5’UTR is a crucial translation determinant. Mutations of this element almost abolished translation efficacy (up to 175-fold decrease), but did not significantly affect mRNA accumulation. The regulatory role of the AU-Box is sequencedependent and might be affected by its inner secondary structure. 4) The internal AUG codon of the psbA 5’UTR is unable to initiate translation. An introduction of mRNA translatability from this codon failed to direct the translation of reporter uidA gene, overriding the mutation of the AU-Box. 5) The 5’end poly(A) sequence does not confer a distinct regulatory signal. The deletion of this element only insignificantly affected mRNA abundance and translation. However, this mutation might slightly disturb the conformation of the stem-loop, resulting in a moderate decrease in translation efficiency (~1.5 fold). 6) The SD(Shine-Dalgarno)-like RBS (ribosome binding site) of the psbA 5’UTR appears to be an indispensable element for translation initiation. Mutation of this element led to a dramatically low expression of the uidA gene as seen by Gus staining. 7) The 5’end structural sequence of the rbcL 5’UTR does not convey a high mRNA stabilising effect to the psbA 5’UTR in a cycling condition of the light and the dark. Their distinct roles appear to be involved in darkness adaptation. Furthermore, with respect to the overall regulatory function of the psbA 5’UTR, two models are proposed, i.e. dual RBS-mediated translation initiation, and cpRBPs-mediated mRNA stability and translation. The mechanisms for mRNA stabilisation entailed by the rbcL 5’UTR are also discussed. Direct repeat-mediated transgene loss after chloroplast transformation and other aspects related to the choice of insertion site and plastid promoter are also analysed.