Podcasts about pagie

In this episode of the Epigenetics Podcast, we talked with Bas van Steensel from the Netherlands Cancer Institute about his work on characterizing chromatin at the Nuclear Lamina. The Interview starts with discussing Bas van Steensel's significant contributions to understanding genome-nuclear lamina interactions. Bas detailed the development of the DAM-ID technique during his postdoctoral studies, which provided a novel way to map genome-wide occupancy and identify Lamina-Associated Domains (LADs). He elaborated on how LADs reveal a distinct domain architecture, often correlating with gene expression levels. This prompted an exploration of the dynamics of these domains during differentiation processes, allowing insights into how gene activation and repression are influenced by their positioning relative to the nuclear lamina. The conversation highlighted the intricate relationship between chromatin dynamics and gene regulation, with Bas sharing compelling findings on how LADs behave during cell differentiation. The research indicated that regions moving away from the lamina often correlated with increased gene expression, revealing a complex interplay of spatial genome organization and transcriptional activity. Additionally, we ventured into the significance of outreach and transparency in scientific research. Bas shared his philosophy regarding collaboration and the ethical responsibility of scientists to share knowledge and resources openly. He emphasized that making lab notebooks and research processes accessible can greatly enhance reproducibility and understanding in the scientific community.   References Open Science Policy of our lab Guelen, L., Pagie, L., Brasset, E., Meuleman, W., Faza, M. B., Talhout, W., Eussen, B. H., de Klein, A., Wessels, L., de Laat, W., & van Steensel, B. (2008). Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions. Nature, 453(7197), 948–951. https://doi.org/10.1038/nature06947 Kind, J., Pagie, L., Ortabozkoyun, H., Boyle, S., de Vries, S. S., Janssen, H., Amendola, M., Nolen, L. D., Bickmore, W. A., & van Steensel, B. (2013). Single-cell dynamics of genome-nuclear lamina interactions. Cell, 153(1), 178–192. https://doi.org/10.1016/j.cell.2013.02.028 Kind, J., Pagie, L., de Vries, S. S., Nahidiazar, L., Dey, S. S., Bienko, M., Zhan, Y., Lajoie, B., de Graaf, C. A., Amendola, M., Fudenberg, G., Imakaev, M., Mirny, L. A., Jalink, K., Dekker, J., van Oudenaarden, A., & van Steensel, B. (2015). Genome-wide maps of nuclear lamina interactions in single human cells. Cell, 163(1), 134–147. https://doi.org/10.1016/j.cell.2015.08.040 Leemans, C., van der Zwalm, M. C. H., Brueckner, L., Comoglio, F., van Schaik, T., Pagie, L., van Arensbergen, J., & van Steensel, B. (2019). Promoter-Intrinsic and Local Chromatin Features Determine Gene Repression in LADs. Cell, 177(4), 852–864.e14. https://doi.org/10.1016/j.cell.2019.03.009 van Schaik, T., Liu, N. Q., Manzo, S. G., Peric-Hupkes, D., de Wit, E., & van Steensel, B. (2022). CTCF and cohesin promote focal detachment of DNA from the nuclear lamina. Genome biology, 23(1), 185. https://doi.org/10.1186/s13059-022-02754-3 van Steensel B. (2018). Scientific honesty and publicly shared lab notebooks: Sharing lab notebooks along with publication would increase transparency and help to improve honesty when reporting results. EMBO reports, 19(10), e46866. https://doi.org/10.15252/embr.201846866   Related Episodes scDamID, EpiDamID and Lamina Associated Domains (Jop Kind) Identification of Functional Elements in the Genome (Bing Ren) Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff)   Contact Epigenetics Podcast on X Epigenetics Podcast on Instagram Epigenetics Podcast on Mastodon Epigenetics Podcast on Bluesky Epigenetics Podcast on Threads Active Motif on X Active Motif on LinkedIn Email: podcast@activemotif.com

On this week's show, Ashlee and Shawn chat about the Britt Baker vs Taya match from Aew Dynamite that everyone seems to be discussing. They take issue with so-called "fans" that tweet bs to Taya, as well as, A "fan" coming up to Saraya and telling her he liked Pagie but not her, then asking for a photo op. They preview the Tag title match between Team Better than you Bay Bay and FTR, plus sing the parties of Will Ospreay.

In this episode of the Epigenetics Podcast, we caught up with Jop Kind from Hubrecht Institute to talk about his work on single cell DamID, EpiDamID, and Lamina Associated Domains (LADs). Jop Kind started out developing single cell DamID (scDamID), based on the DamID technique. First, this technique was adapted to a microscopic readout which enabled them to follow the localisation of chromatin domains after cell division. Next, the lab expanded this technique into the NGS space and created genome-wide maps of nuclear lamina Interactions in single human cells. Since LADs are in a heterochromatic chromatin context, the lab expanded scDamID into the epigenetic space. They first combined it with a transcriptional readout.  Later-on they developed EpiDamID, a method to target a diverse set of chromatin types by taking advantage of the binding specificities of single-chain variable fragment antibodies, engineered chromatin reader domains, and endogenous chromatin-binding proteins.   References Kind, J., Pagie, L., Ortabozkoyun, H., Boyle, S., de Vries, S. S., Janssen, H., Amendola, M., Nolen, L. D., Bickmore, W. A., & van Steensel, B. (2013). Single-Cell Dynamics of Genome-Nuclear Lamina Interactions. Cell, 153(1), 178–192. https://doi.org/10.1016/j.cell.2013.02.028 Kind, J., Pagie, L., de Vries, S. S., Nahidiazar, L., Dey, S. S., Bienko, M., Zhan, Y., Lajoie, B., de Graaf, C. A., Amendola, M., Fudenberg, G., Imakaev, M., Mirny, L. A., Jalink, K., Dekker, J., van Oudenaarden, A., & van Steensel, B. (2015). Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells. Cell, 163(1), 134–147. https://doi.org/10.1016/j.cell.2015.08.040 Borsos, M., Perricone, S.M., Schauer, T. et al. Genome–lamina interactions are established de novo in the early mouse embryo. Nature 569, 729–733 (2019). https://doi.org/10.1038/s41586-019-1233-0 Markodimitraki, C. M., Rang, F. J., Rooijers, K., de Vries, S. S., Chialastri, A., de Luca, K. L., Lochs, S. J. A., Mooijman, D., Dey, S. S., & Kind, J. (2020). Simultaneous quantification of protein–DNA interactions and transcriptomes in single cells with scDam&T-seq. Nature Protocols, 15(6), 1922–1953. https://doi.org/10.1038/s41596-020-0314-8 Rang, F. J., de Luca, K. L., de Vries, S. S., Valdes-Quezada, C., Boele, E., Nguyen, P. D., Guerreiro, I., Sato, Y., Kimura, H., Bakkers, J., & Kind, J. (2022). Single-cell profiling of transcriptome and histone modifications with EpiDamID. Molecular Cell, 82(10), 1956-1970.e14. https://doi.org/10.1016/j.molcel.2022.03.009   Related Episodes Dosage Compensation in Drosophila (Asifa Akhtar) Chromatin Profiling: From ChIP to CUT&RUN, CUT&Tag and CUTAC (Steven Henikoff) Single Cell Epigenomics in Neuronal Development (Tim Petros)   Contact Epigenetics Podcast on Twitter Epigenetics Podcast on Instagram Epigenetics Podcast on Mastodon Active Motif on Twitter Active Motif on LinkedIn Email: podcast@activemotif.com

Interview met Nathalie Pagie. Zij debuteerde in 2013 met De toneelclub. In september verscheen haar tiende thriller Camping Oosthoek.  Komende najaar verschijnt van haar hand Vuurduivel.

Paige Birgfeld was a mother of three who was adored by children and by her close knit group of friends. Recently divorced from her second husband--the father of her three kids--she was working multiple jobs to make ends meet while raising the kids on her own. Paige's last known contact was a cell phone call to her first husband around 9pm on June 28th 2007. And then she vanished without a trace. As police investigate her disappearance, they discover that Pagie had been working as an adult escort--which escelates their fears that she may have met with foul play. THIS ONE HAS A RESOLUTION, YOU GUYS! IN THE END WE FIND OUT WHAT HAPPENED! YOU GUYS, WE'RE GOING LIVE ON OUR INSTAGRAM PAGE (@TheDisappearedPod) THIS AND EVERY FRIDAY AT 6PM! Come join us for #FridayNightLive where we'll chat about the case, take your questions, and just hang for a bit! JOIN US!!

I invited my sister Pagie & my newest friend Katie to discuss dinner dates. I personally hate them, but the stories are pretty funny. --- Send in a voice message: https://anchor.fm/jace98/message Support this podcast: https://anchor.fm/jace98/support

This week I'm talking with Paige Hull of The Vintage Round Top, a beautiful retreat space in the heart of antiquer's heaven in Round Top, TX.  Pagie and her husband Smoot have put a lot of love and soul into it, starting with a gut renovation of the first house, "No. 1450," and then adding another they call "Boho," and most recently two small cottages named after their children, The Pierce and The Eisley.   This magical place is where you can join me on the next Little Yellow Couch style retreat so be sure to check that out on the website, but today, I really wanted to give Paige the floor to share why and how she designs her homes with antiques.  If you're a true vintage lover, this episode will naturally resonate with you.  But if you skew more modern, I'm particularly excited for you to hear what Paige has to say.  Because I think she and Smoot have really nailed it when it comes to incorporating a few chipped and scarred pieces in the most clean-lined, minimalist of spaces.  Proving antiques really are for everyone.  

Paige Stewart, one of Funky Feminist's most popular writers, joins Landon to continue the conversation about sex education. Hailing from South Africa, Queen Stewart offers fresh insight and vulnerability about sexuality and how hers has unraveled over the past few years. The two discuss everything from discrimination to animal rights. The two discuss: Hot Girls Wanted: https://www.imdb.com/title/tt4382552/ Hot Girls Wanted: Turned On DocuSeries: https://www.imdb.com/title/tt6333104/?ref_=tt_rec_tt Bellesa: www.bellesa.co To contact Pagie, send her a DM on Instagram @plantiful_paige. Links to her work for Funky Feminist: https://www.funkyfeminist.com/blog/vegan-rights https://www.funkyfeminist.com/blog/shaming-slut-shaming https://www.funkyfeminist.com/blog/shaving-who-needs-it https://www.funkyfeminist.com/blog/swiping-your-v-card https://www.funkyfeminist.com/blog/wtf-is-meninism --- Support this podcast: https://anchor.fm/funky-feminist/support

This week Erin & Stella breakdown their feeling about NXT Takeover: Wargames & Survivor Series. tldr: Pagie is back, Drewboo is hurt, Velveteen Dream proved us wrong, HHH loves himself A LOT, wrestling is emotional. Enjoy your turkey Demmies!! Follow us! @Stella_Cheeks @ErnGenC #NotYourDemoPod www.cagesideseats.com www.notyourdemographic.tumblr.com  

Pagie is just person that is doing what she loves! I really admire her intensity & passion for bodybuilding and Nutrition! we discuss many topics steroids, her story and much more.Check Out Her      https://www.instagram.com/beefnuggette/?hl=en --- Support this podcast: https://anchor.fm/thetwoamericans/support

WELL THE INTERNET JUST BROKE WE TALK NEWS IN MOVIES TV, ANIME, AND VIDEO GAMES AND A TOP 10 OF BEST N64 GAMES