Podcasts about caestecker

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.07.13.548868v1?rss=1 Authors: de Caestecker, C., Macara, I. Abstract: Apical sorting of epithelial membrane proteins is an essential process but remains incompletely understood. Apical cytoplasmic domains are significantly smaller than those of basolateral proteins; however, the reason for this attribute is unknown. We asked if a diffusion barrier at the trans-Golgi network might impede apical sorting of proteins with large cytoplasmic tails. We used Crumbs3 and Ace2 as example apical transmembrane proteins with short cytoplasmic tails. FKBP was attached to the C-termini for inducible dimerization to FRB-tagged proteins. A streptavidin-binding peptide on the extracellular domain traps the proteins in the endoplasmic reticulum (ER). Biotin addition triggers release to the Golgi, then departure in vesicles to the apical cortex. Increasing cytoplasmic bulk by dimerization to FRB-SNAPtag moieties significantly delayed departure. Crb3 binds through its cytoplasmic tail to the Pals1 protein, and although Crb3 and Pals1 are associated at the ER and Golgi, Pals1 disassociates before Crb3 departure. A non-dissociable mutant Pals1 impedes Crb3 exit. We conclude that small cytoplasmic domains facilitate apical sorting. Reducing cytoplasmic domain size by timely Pals1 release is essential for normal kinetics of Crb3 sorting. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.06.531292v1?rss=1 Authors: Larsen, L. E., Caestecker, S., Stevens, L., van Mierlo, P., Carrette, E., Boon, P., Vonck, K., Raedt, R. Abstract: The locus coeruleus (LC), a brainstem nucleus, is the sole source of noradrenaline in the neocortex, hippocampus and cerebellum. Noradrenaline is a powerful neuromodulator involved in the regulation of excitability and plasticity of large-scale brain networks. In this study, we assessed the activity of locus coeruleus neurons and changes in noradrenergic transmission during acute hippocampal seizures evoked with perforant path stimulation. LC neurons were recorded in anesthetized rats using a multichannel electrophysiology probe and were identified based on electrophysiological characteristics or optogenetic tagging. The majority of LC neurons (55%) were inhibited during seizures, while only a subset of LC neurons (28%) was excited during seizures. Topographic analysis of multi-unit activity showed anatomical separation of neurons that were excited and inhibited during seizures. Changes in hippocampal noradrenaline transmission during seizures were assessed using a fluorescent biosensor for noradrenaline, GRABNE2m, in combination with fiber photometry in both anesthetized and awake rats. Our results indicate that acute electrically evoked hippocampal seizures are associated with strong changes in LC unit activity and strong and consistent time-locked release of noradrenaline. Understanding the role of mass release of noradrenaline during hippocampal seizures is likely to be important to understand seizure pathophysiology. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

By Ben Lewis. A witty and propulsive six-part thriller about an exclusive personal development programme with a radical twist. Starring Siena Kelly, Jack Rowan and Iain de Caestecker. Level 4: Red Pill The Past: Jake's unit find out what The System is really about as they enter the next level of their training. And it doesn't sit well with everyone. The Present: Maya manages to track down an ally with insider knowledge. Cast: Alex … Iain de Caestecker Dr Khalid Hassan… Souad Faress Maya … Siena Kelly Coyote…Divian Ladwa Beau…Matthew Needham Jake …Jack Rowan Original music and sound design by Danny Krass Featuring tracks from Equiknoxx music collective With thanks to Dr Joel Busher at the Centre for Trust, Peace and Social Relations, University of Coventry. A BBC Scotland Production directed by Kirsty Williams

By Ben Lewis A propulsive thriller starring Siena Kelly, Jack Rowan and Iain de Caestecker. Level 1: Get Naked. When Jake signs up to a personal training programme that promises to turn the meekest man-boy into an alpha male, he doesn't have a clue what he's getting himself into. Two years later, his sister Maya sets out to uncover the truth. Cast: Alex … Iain de Caestecker Jerome… Don Gilét Maya … Siena Kelly Beau…Matthew Needham DI Cohen …Chloe Pirrie Jake …Jack Rowan Original music and sound design by Danny Krass Featuring tracks from Equiknoxx music collective With thanks to Dr Joel Busher at the Centre for Trust, Peace and Social Relations, University of Coventry. A BBC Scotland Production directed by Kirsty Williams

By Ben Lewis. A witty and propulsive six-part thriller about a mysterious personal development programme. Starring Siena Kelly, Jack Rowan and Iain de Caestecker. Level 2: Sweat Blood. The Past: Jake and his fellow underdogs begin to radically transform their minds and bodies. The Present: Maya's hunt for their leader gets tense when she realises they might be hunting her. Cast: Alex … Iain de Caestecker Maya … Siena Kelly Jerome… Don Gilét Coyote…Divian Ladwa Beau…Matthew Needham Jake …Jack Rowan Original music and sound design by Danny Krass Featuring tracks from Equiknoxx music collective With thanks to Dr Joel Busher at the Centre for Trust, Peace and Social Relations, University of Coventry. A BBC Scotland Production directed by Kirsty Williams

By Ben Lewis A witty and propulsive six-part thriller about an exclusive personal development programme with a radical twist. Starring Siena Kelly, Jack Rowan and Iain de Caestecker. Level 3: Field Work The Past: pumped up and fighting fit, Jake and his unit learn how to hunt. And it's not just animals they're in search of. The Present: Maya meets a woman she hopes can explain why Jake's life hangs in the balance. Cast: Alex … Iain de Caestecker Maya … Siena Kelly Coyote…Divian Ladwa Beau…Matthew Needham DI Cohen… Chloe Pirrie Grace…Ashna Rabheru Jake …Jack Rowan Original music and sound design by Danny Krass With thanks to Dr Joel Busher at the Centre for Trust, Peace and Social Relations, University of Coventry. A BBC Scotland Production directed by Kirsty Williams

By Ben Lewis. A witty and propulsive six-part thriller about a group of young radicals and the hunt for their leader. Starring Siena Kelly, Jack Rowan and Iain de Caestecker. Level 5: Kill the Past The Past: Jake and Alex have a stand-off when Jake's given a target dangerously close to home. The Present: Maya's TV habit, once described as her downfall, is about to become her greatest asset. Cast: Alex … Iain de Caestecker Jerome… Don Gilét Maya … Siena Kelly Coyote…Divian Ladwa Beau…Matthew Needham DI Cohen / Jess …Chloe Pirrie Jake …Jack Rowan Original music and sound design by Danny Krass Featuring tracks from Equiknoxx music collective With thanks to Dr Joel Busher at the Centre for Trust, Peace and Social Relations, University of Coventry. A BBC Scotland Production directed by Kirsty Williams

By Ben Lewis. A witty and propulsive six-part thriller about a group of young radicals and the hunt for their leader. Starring Siena Kelly, Jack Rowan and Iain de Caestecker. Level 6: Judgement Day Question Mark The Past: Alex's story comes into focus. The Present: Maya goes in search of Jake, hoping together they can stop the System before things get totally out of control. But can they? And who's really in charge? Cast: Alex … Iain de Caestecker Maya … Siena Kelly Coyote…Divian Ladwa Beau…Matthew Needham DI Cohen / Jess …Chloe Pirrie Jake …Jack Rowan Original music and sound design by Danny Krass Featuring tracks from Equiknoxx music collective A BBC Scotland Production directed by Kirsty Williams With thanks to Dr Joel Busher at the Centre for Trust, Peace and Social Relations, University of Coventry.

When Jake goes on the run after a mysterious crime, his sister Maya must find out why. A propulsive six-part thriller. Starring Siena Kelly, Jack Rowan and Iain de Caestecker.

Warning: This episode contains spoilers for Episode Two of the First Season of Roadkill. The role of a special adviser in British politics is complicated — but Roadkill star Iain de Caestecker does quick work of explaining the job, and his character, Duncan Knock, in a charming interview. As to whether or not viewers should trust Duncan? Well, that's still a secret.

Agents of SHIELD Season 6 Episode 6 Review: Inescapable A bottle episode of Agents of SHIELD never scared anyone, and seeing Fitz and Simmons reconcile all of their pain in "Inescapable" really highlighted the acting of Iain de Caestecker and Elizabeth Henstridge. Although the story didn't move forward much, the psychological experiment was worth the price of admission! Join us for show news, commentary and analysis for "Inescapable," our listener feedback segment, and the return of the renamed SHIELD Sass Report! This week we talk about memories of Coulson, the inexplicable actions of the Chronicoms, the surprising appearance of a "shadow" Simmons, the reprisal of Dr. Fitz, and much more! Remember to join the Agents of SHIELD (and other Marvel properties) discussion on Facebook and follow us on Twitter for the latest news and live tweets during the episode. And don't forget to vote for your favorite CoulSass moment in the Facebook group as well! Tell us what you think about Agents of SHIELD! We'd love to hear your thoughts about Agents of SHIELD on a future episode of The Sandbox! Use our Feedback page or call 304-837-2278 to tell us your opinions, theories, and general feelings. And while you're at it, why not take a few minutes to leave a rating and review in iTunes? Agents of SHIELD Inescapable Show Notes TVLine's Performers of the Week: Iaian de Caestecker and Elizabeth Henstridge Taltos' screenshots of Shadow Simmons    

Dr John Simon de Caestecker discusses his article, "Radiofrequency ablation compared with argon plasma coagulation after endoscopic resection of high-grade dysplasia or T1 adenocarcinoma in Barrett's esophagus: a randomized pilot study (BRIDE)" from the April issue.

Check out Linda's bio and recommended careers resources: www.gla.ac.uk/alumni/welcomehome/soundtracks/lindadecaestecker/ UofG Grad Linda De Caestecker is the Director of Public Health for Greater Glasgow and Clyde, a career which she moved into after working as a Doctor both at home and abroad.  Now an Honorary Professor, Charity Trustee and important local figurehead, Linda explains what Public Health really means in it’s broadest sense – and how you can build a career in it too.  We talk through some of the big health issues that shape all our lives and hear Linda's big career takeaways (not the Friday night pizza variety!) Listen out for: Linda’s insight into types of leaders, advice on getting into Public Health no matter what you studied – plus, her confession in a very relatable Myth Buster! What’s your soundtrack?: We ask Jamie, a UofG Future World Changer student with a mission to bring Love back into the Care System. Sound Tracks is supported by The Network - UofG's exclusive online community for students, graduates and staff. Join at bit.ly/TheUofGNetwork

Jimmy sat in on the roundtables for AGENTS OF SHIELD. He chatted with stars Henry Simmons, Natalia Cordova-Buckley, Chloe Bennett, Clark Gregg, Elizabeth Henstridge, Iain De Caestecker, Ming Na Wen, and producer Jeph Loeb. What's gonna happen in space? Will they run into any other Marvel comics characters while up there? Is a crossover with the new Inhumans show coming? Listen in to find out! Leave your iTunes comments! 5 stars and nothing but love! Also, get a hold of us! Thanks for listening!

Iain de Caestecker talks about Fitz's new role as the Head of Hydra in the Framework! Catch new episodes of “Marvel’s Agents of S.H.I.E.L.D.” Tuesdays at 10:00 PM ET on ABC.

At San Diego Comic Con, Jimmy sat in on the roundtables for Marvel's Agents of S.H.I.E.L.D. Among the cast/creative participants were Chloe Bennett, Elizabeth Henstridge, Clark Gregg, Henry Simmons, Ming-Na Wen, Iain De Caestecker, and Producers Jeph Loeb, eff Bell. You'll hear about the upcoming season 4. Will we hear about what happens after the 6 month time jump? How is Ghostrider joining the series? What's up with Daisy's new goth look? How will the team find its way back to each other? Will old characters return? You'll have to tune in to find out! Leave your iTunes comments! 5 stars and nothing but love! Also, get a hold of us! Thanks for listening!

Iain de Caestecker joins "This Week in Marvel's Agents of S.H.I.E.L.D." to discuss Fitz's search for Simmons, the character's new confidence, and much more! Catch new episodes of "Marvel's Agents of S.H.I.E.L.D." Tuesdays at 9:00 PM ET on ABC.