Podcasts about tm4

Fr. Timothy Gallagher and Kris McGregor discuss the often overlooked relationships that shaped the life and spirituality of St. Thérèse of Lisieux. Reflecting on the profound and enduring influence of Thérèse's extended family, Fr. Gallagher takes a special focus on her cousin Marie Guérin, who was both a close friend and spiritual companion to Thérèse. The post TM4 – Overcoming Scruples – St. Therese and Marie: The Story of Two Cousins with Fr. Timothy Gallagher – Discerning Hearts Podcast appeared first on Discerning Hearts Catholic Podcasts.

For this episode Ryan Maughan speaks to EV industry expert Eric Azeroual, who is VP of the Danfoss Editron On-Highway Business Unit.Eric has been involved in the electrification industry since 2004, when after graduating from Polytechnique Montreal with a degree in mechatronic engineering, he joined what was TM4, an early pioneer in the electric vehicle industry. TM4's history started in the early '90s, Hydro-Québec, the forward looking power generation utility for the Canadian province of Quebec, had a research and development team that was experimenting in electric vehicle drivetrain systems trying to develop technology that would make cleaner alternatives for vehicle powertrains viable. With some notable successes leading to the TM4 business being created in 1998. In 2018 the business received a major investment from the Tier 1 transmissions company Dana, where Eric became head of global electrification sales.Eric left Dana to join the team at Danfoss Editron heading up the on-highway business unit in July '21. Danfoss Editron is the division of the major global Tier 1 Danfoss focused on heavy-duty electric powertrain solutions for mobile applications in marine, off-highway and on-highwayRyan and Eric discuss the history of the electric commercial vehicle space and Eric's experiences and his outlook on what is looking like an exciting future within the Danfoss business. Including how the Editron business origins and the continued investment in the area with the recently announced merger between Danfoss Silicon Power and Semikron.Eric Azeroual LinkedIn profile: https://www.linkedin.com/in/eric-azeroual-9b093a5/Danfoss Editron website: https://www.danfoss.com/en/about-danfoss/our-businesses/power-solutions/danfoss-editron/Ryan Maughan LinkedIn profile: https://www.linkedin.com/in/ryan-maughan-a2893610/Ryan Maughan on Twitter: https://twitter.com/acexryaneTech49 website: https://www.etech49.comeTech Developments website: https://www.etechdevelopments.com

Pedagog zostaje zastrzelony we własnym łóżku. Policja szybko odkrywa, że Chuck Leonard miał wielu wrogów. Sprawa mogłaby ciągnąć się miesiącami, gdyby nie zeznanie przyjaciółki jego żony. źródła pod filmem: https://www.youtube.com/watch?v=5bpuj7-_tM4&lc=UgxwiCGaJxO5ZvyZS5F4AaABAg

http://www.sterlingrobertsmusic.com/#about Ask this Multiple ABGMA 2020 Award Winner & Multiple International Nominee, Trinidad-born, Singer-Songwriter, Background Vocalist, Worship leader, Spoken Word & Gospel Recording Artiste about his credentials & Sterling Roberts would tell You he's eternally grateful, but not satisfied by his abilities neither by mere earthly accolades… With a testimony of being healed from a deteriorating heart condition at 8 years old & many other miraculous rescues, Sterling's desire in & through his ‘edgy, intense-intimate' sound, is beyond the natural. His aim is to be a bridge; a divine pathway used to transport The Same Supernatural Love that invaded His life when he was in a desolate place… and brought redemption! Sterling Roberts, is a new generation worshiper, who brings the presence of God in his sets with uplifting lyrics and energetic performances. Sterling has been a lead worshiper in his home church, The Apostles Ministries for almost a decade and has no intentions of slowing down. Working as part of a dynamic team of vocalists & musicians called, TAM Worship, Sterling has led various sets alongside renowned worship leaders like Jaye Thomas, Maranda Curtis, Anthony Brown & Group Therapy, Gates Praise and much more. As a member of TAM Worship he has also had the privilege of performing on big stages such as Open House 2017. Straight from the ink of his heart onto paper, Sterling Roberts paints a picture and sends a message full of salt and light to listeners through his spoken word. Having performed in various spoken word stages including “One Voice – The Uprising” and “Christ Check: Poetry & Jazz Night”, Sterling's lyrics are full of hidden gems and wordplay. Listeners need a second take to grab the multi-layered messages spread through the carefully knit lyrics of this Christ-like poet. Gifted with the singing ability & years of experience in training & performance, Sterling Roberts is a known vocalist in many circles. Having performed with & supported various artistes such as Samuel Medas, Jaron Nurse, Nikki Rymple, Blessed Messenger, Jedidiah, he has performed in concerts, to name a few: “Switch – The Concert” with the team B.A.S.S (Born Ambassadors Saving Souls), Liberation Day 2018 & GlowFest Tobago 2018, with the team of renowned vocalists, nicknamed TM4.

http://www.sterlingrobertsmusic.com/#aboutAsk this Multiple ABGMA 2020 Award Winner & Multiple International Nominee, Trinidad-born, Singer-Songwriter, Background Vocalist, Worship leader, Spoken Word & Gospel Recording Artiste about his credentials & Sterling Roberts would tell You he's eternally grateful, but not satisfied by his abilities neither by mere earthly accolades…With a testimony of being healed from a deteriorating heart condition at 8 years old & many other miraculous rescues,Sterling's desire in & through his ‘edgy, intense-intimate' sound, is beyond the natural.His aim is to be a bridge; a divine pathway used to transport The Same Supernatural Love that invaded His life when he was in a desolate place… and brought redemption!Sterling Roberts, is a new generation worshiper, who brings the presence of God in his sets with uplifting lyrics and energetic performances. Sterling has been a lead worshiper in his home church, The Apostles Ministries for almost a decade and has no intentions of slowing down. Working as part of a dynamic team of vocalists & musicians called, TAM Worship, Sterling has led various sets alongside renowned worship leaders like Jaye Thomas, Maranda Curtis, Anthony Brown & Group Therapy, Gates Praise and much more. As a member of TAM Worship he has also had the privilege of performing on big stages such as Open House 2017.Straight from the ink of his heart onto paper, Sterling Roberts paints a picture and sends a message full of salt and light to listeners through his spoken word.Having performed in various spoken word stages including “One Voice – The Uprising” and “Christ Check: Poetry & Jazz Night”, Sterling's lyrics are full of hidden gems and wordplay. Listeners need a second take to grab the multi-layered messages spread through the carefully knit lyrics of this Christ-like poet.Gifted with the singing ability & years of experience in training & performance, Sterling Roberts is a known vocalist in many circles. Having performed with & supported various artistes such as Samuel Medas, Jaron Nurse, Nikki Rymple, Blessed Messenger, Jedidiah, he has performed in concerts, to name a few: “Switch – The Concert” with the team B.A.S.S (Born Ambassadors Saving Souls), Liberation Day 2018 & GlowFest Tobago 2018, with the team of renowned vocalists, nicknamed TM4.

Même si aucun modèle n’y est construit à grande échelle, le Québec a eu son rôle à jouer dans le développement de l’automobile. Et ça commence pas mal plus tôt que vous pourriez le croire!

La iPace de Jaguar saura impressionner, TM4 passe aux mains de Dana Corp., la première 100% électrique de Volvo sera la XC40, Flo étend son réseau en… Lire la suite "Silence on roule – Épisode # 37 (07/2018)"

Breaking from the usual technical episodes we just had to bring you an update on some of the major EV industry newsflashes from the past few weeks brought to you by AVID Technology MD and industry expert Ryan Maughan. In this episode learn more about BP's acquisition of UK company Chargemaster, major tier 1 company Dana's purchase of TM4, Volkswagen setting a new lap time record at Pikes Peak with an EV and Porsche's EV the 919 setting a new record at Nurburgring, TESLA's breaking production news, and Cummins acquisition of Efficient Drivetrains (EDI). So much packed into one short episode!For regular EV industry news we recommend you sure to listen to the EV News Daily Podcast by Martyn Lee.To read more about the news discussed in this episode check out the links below:https://techcrunch.com/2018/06/20/porsche-just-took-a-10-stake-in-electric-hypercar-maker-rimac/https://www.tm4.com/news-events/tm4-is-now-a-part-of-dana-inc-with-hydro-quebec-remaining-as-a-key-partner/http://www.autoexpress.co.uk/volkswagen/101402/vw-to-attack-pikes-peak-with-new-electric-racerhttps://www.bbc.co.uk/news/business-44640647https://arstechnica.com/cars/2018/06/porsche-shatters-the-nurburgring-record-we-thought-was-unbreakable/https://www.marketwatch.com/story/tesla-stock-soars-after-hitting-5000-model-3-production-target-and-shooting-for-6000-2018-07-02https://www.businesswire.com/news/home/20180702005572/en/Cummins-Announces-Acquisition-Electric-Hybrid-Powertrain-ProviderSubscribe, rate and don't miss an episode!AVID Technology website: https://avidtp.com/AVID's YouTube Page: https://www.youtube.com/channel/UCJdGkqGQvMSXxNC-4p8j3hA

Bill Jacobs talks about developing mycobacterial genetic tools and using them to discover ways to shorten TB treatment. He also talks about the SEA-PHAGES program that allows high-school students to participate in phage discovery. Host: Julie Wolf Subscribe (free) on iPhone, Android, RSS, or by email. You can also listen on your mobile device with the ASM Podcast app. Julie's biggest takeaways: The challenges of working with an easily aerosolized bacterium are aided by complementary studies on a noninfectious relative. M. smegmatus doesn’t colonize mammals and grows slower, giving researchers the opportunity to acclimate themselves to working with mycobacterial cultures. Jacobs was the first scientist to introduce DNA into M. tuberculosis using a phasmid - part plasmid, part mycobacterial phage. The first phage came from Jacobs’ dirt yard in the Bronx, so he named it BxB1 for the Bronx Bomber. Another phage, TM4, became the workhorse phasmid when Jacobs cloned an E. coli cosmid sequence into a nonessential part of the phage genome. It replicates in E. coli as a plasmid but becomes a phage inside Mycobacteria, facilitating manipulation. The shuttle phasmids allowed transposon delivery to make transposon libraries, and the creation of gene knockouts. To this day, we use Ziehl-Neelsen staining to differentiate acid-fast mycobacteria from gram-positive or gram-negative bacteria - the mycolic acids on the outer part of the envelope make up some of the longest microbial lipid chains. But mycobacteria can regulate its acid-fast positive or negative status; the acid-fast negative organisms are a persistent population that are often ignored inside of patients. 99.99% of M. tuberculosis bacteria are not persistent, but the last 0.1% have entered into a persistent state expressing many stress proteins that help them become refractory to killing. A normal course of antibiotic chemotherapy for patients is six months. If infected with a strain resistant to the two frontline drugs, that time goes up to two years. The problem is even greater in extremely multidrug resistant (XDR) strains. What we really need is a way to understand persistence and a way to shorten chemotherapy. That’s why were were absolutely amazed when we discovered that cysteine with isoniazid completely sterilizes Mtb cultures in vitro and in vivo! The culture is sterilized because the bacteria can’t form persisters. Vitamin C co-treatment with antibiotics may lead to a shortened course of therapy for TB treatment. Neutralizing antibodies to the herpesvirus glycoprotein have been the dogma for protecting from herpes. Jacobs and his colleagues discovered that a vaccine based on a glycoprotein-knockout virus confers sterilizing immunity not through neutralizing antibodies but through antibody-dependent cell cytoxicity (ADCC). This ADCC response may also be important to develop a more effective TB vaccine.   Featured Quotes (in order of appearance): “You’ll never know how bad your aseptic technique is until you start working with tuberculosis!” “I think part of the reason I had the opportunity to develop genetics for TB - it’s not like it wasn’t important to do - but a lot of people were disappointed when working with the organism.” “We’re about to take TB genetics to where yeast genetics is.” “One of the tubicle bacilli’s greatest powers or one of its most important phenotypes is that it has the ability to persist, which means it has the ability to tolerate killing effectors, either killing by the immune system or killing by bactericidal drugs.” “I took students to the Bronx Zoo, and over by the zebra pen, I sniffed and said ‘I smell a phage!’ In fact, that’s not crazy - anyone who plants flowers knows what good soil smells like, and in the good soil, you’re smelling the bacteria that live in the soil, the Streptomyces and Mycobacteria. I reached down and grabbed that dirt, and when we went back to work we isolated BxE1.” “I’ve never met a phage I wasn’t excited about!” “I now believe that most pathogens do not ‘want’ ADCC antibodies to be made, and they have immune evasion strategies where they skew the immune response to get the wrong antibodies. Since the time we published our first paper, numerous groups have shown that correlates of protection for HIV, for influenza, and for Zika, turn out to be ADCC antibodies.” “Genetics is the mathematics of biology!”   Links for this episode Bill Jacobs lab site NYTimes story on 1993 rapid diagnostic test using luciferase AACJournal: Vitamin C potentiates the killing of Mycobacterium tuberculosis by the first-line tuberculosis drugs isoniazid and rifampicin in mice Cell: Origins of highly mosaic mycobacteriophage genomes SEA-PHAGES program eLife: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity mBio: Dual-reported mycobacteriophages (Φ2DRMs) reveal preexisting Mycobacterium tuberculosis persistent cells in human sputum Tuberculosis - Its cause, cure and prevention [1914] (pdf)   Send your stories about our guests and/or your comments to jwolf@asmusa.org.  

The tetraspanin peripherin-2 is a glyco-membrane protein exclusively expressed in the outer segments of rod and cone photoreceptors. Mutations in peripherin-2 are associated with retinal disorders characterized by Degeneration of rod or cone cells. Previous unpublished work identified peripherin-2 as a potential novel part of the protein complex comprising the B-subunit of the cyclic nucleotide-gated channel (CNGB1a and the light detector rhodopsin. In the first part of this study, using a combination of protein biochemical and FRET approaches in transfected HEK293 cells and in virally transduced murine rod outer segments, it could be demonstrated that peripherin-2 simultaneously binds to both, CNGB1a and rhodopsin. The interaction between peripherin-2 and rhodopsin was not described in previous studies. The binding domain mediating the peripherin-2/rhodopsin interaction could be narrowed down to the fourth transmembrane domain (TM4) of peripherin-2. Finally, the data revealed that the G266D point mutation in TM4 of peripherin-2 that is linked to a rod degenerative disease selectively disrupts the peripherin-2/rhodopsin interaction. To analyze if peripherin-2 also binds to cone opsins in the second part of this study, a similar experimental approach was conducted as used for the investigation of the peripherin-2/rhodopsin interaction. In this context, it was unveiled that peripherin-2 binds to both, short wavelength-and medium wavelength-sensitive cone opsin (S-opsin and M-opsin, respectively) in transfected HEK293 cells and in outer segments of transduced murine cones. Co-immunoprecipitation and quantitative FRET analysis revealed that binding of peripherin-2 to M-opsin was stronger than the peripherin-2/S-opsin interaction. This result was supported by transmission electron microscopy studies using gold particles coupled to opsin- and peripherin-2-specific antibodies. Finally, quantitative FRET analysis in transfected HEK293 cells and in transduced cone outer segments demonstrated that the V268I Point mutation in TM4 of peripherin-2 associated with a degenerative cone disease significantly attenuates the peripherin-2/M-opsin interaction. Taken together, this study provides a proof-of-principle for FRET-based analysis of protein-protein interactions in the outer segments of rod and cone photoreceptors. This approach led to the identification of hitherto unknown Protein complexes between peripherin-2 and opsins suggesting a novel physiological role of peripherin-2 in rods and cones. Finally, Analysis of disease-linked point mutations unveiled the molecular determinants of the peripherin-2/opsin interaction. These results might contribute to understanding the differential penetrance of certain point mutations in rods and cones.