Podcasts about dctp

We're mixing it up for our 75th episode extravaganza! We've watched a lot of DCOMs, and even though we haven't seen them all, we thought it would be fun to take our 16 favorite movies and pit them against each other until there's only one left: the #1 DCOM, better than all the others. So go make your favorite Tipsy Panel cocktail and join us to find out if our #1 movie is yours too! Instagram: @disneychanneltipsypanel Twitter: @TipsyPanel Email us: disneychanneltipsypanel@gmail.com DCTP Hotline: (914) 863-1613

In this week's episode Daria and Jenny are recapping the three part miniseries Death Comes to Pemberley with pod friend HEAapologist aka Bre! DCTP is a murder mystery, Regency style, that takes place in the lives of Fitzwilliam Darcy and his wife, Elizabeth (nee Bennet), six years after the events of Pride and Prejudice. This three part BBC miniseries is not just a murder mystery, but also gives us a look into the many different kinds of relationships and love that exist - like the new love blooming between Henry and Georgiana, love that has stood the test of time between Mr. and Mrs. Bennet, love that finds its way in the sometimes fraught but always fun relationship between Wickham and Lydia. And most importantly, the love and friendship between Darcy and Lizzie, that once again endures through the trials and tribulations put on them by her family.  We were so excited to have Bre on the podcast to listen to all of her thoughts on Darcy, Lizzie, Mrs. Bennet's heart palpitations and what an absolute asshole Wickham continues to be! We also talk about our book of the week, Casey McQuiston's One Last Stop, with honorable mentions to Kate Canterbary's Worst Guy and Sara Whitney's soon to be released second chance romance, Tempting Fate.  Bre recommends checking out the comedy series, Murderville, starring Will Arnett, with some great guests including Conan O'Brien, Annie Murphy and Ken Jeong.  Don't forget to vote in the Darcy Bracket on Twitter and Instagram!  You can find Bre on Twitter @HEAapologist! As always, please Subscribe/Rate/Review! If you liked the podcast recommend us to your friends!  You can follow our socials: Instagram @hotelvicarious Twitter @hotelvicarious Facebook https://www.facebook.com/hotelvicarious

Happy holidays from the DCTP

We're back! Guest star Torie Burmeister joins the DCTP for their triumphant return with the very first DCOM, featuring Bill Fagerbakke (Spongebob Squarepants) as a bumbling mummy. Featured drink: The Mummy (or the Bloody Mummy, if you're feeling frisky)

Hey y'all, This is your part two! Hopefully you've JUST listened to DCTP episode 2. If not, we think you'll have fun anyway.  Lots o love, Allison, Julie, and Janine 

Fifty episodes means another Quizney Channel Tipsy Panel!! Everybody came in with a question round, and we called in a few old friends to quiz us as well. We play DCOMmentary, What's in a Name, DCOM quotes, and more! Plus, Ryan shares some calculated DCTP stats, and an old friend moderates the first-ever DCOM Debates.

You know we had to do the big one for our 50th episode! This movie beat out Monday Night Football for the most watched basic cable broadcast in 2007, which solidifies our belief that DCOMs are better than sports. James teaches us how to make a Lava Lemonade (tasty tasty) and we talk about how much watching 50 Disney movies has rotted our brains. We also share some DCTP stats, and you might be surprised to find out who's given the fewest thumbs ups...

Podcast o grach wideo i komputerowych. W tym odcinku rozmawiamy głównie o: - Disco Elysium - Star Wars: Jedi Fallen Order Wszystkie opinie wygłaszane przez nas w podcaście są naszymi prywatnymi opiniami i w żadnym wypadku nie są stanowiskiem firm, organizacji i instytucji, których pracownikami, współpracownikami lub członkami jesteśmy. Zapraszamy na: - nasz fanpage: www.facebook.com/WszystkoGraTV/ - grupę Wszystko Gra: www.facebook.com/groups/616626868494911/ LINK do podcastu WYDAWCY HAJSU: https://www.facebook.com/wydawcyhajsu - serwer Discord: discord.gg/YRktsyh - stronę: www.wszystkogra.tv www.rozgrywkapodcast.pl - instagram: wszystko_gra_tv - bloga Ani: www.landminecat.pl/ - kanał Milo: www.youtube.com/user/kislawala - wydarzenie Kasi: www.facebook.com/events/135063713…/?__mref=message LINK do podcastu WYDAWCY HAJSU: https://www.facebook.com/wydawcyhajsu

In this very special episode of DCTP, we drink Lone Stars and champagne while we review the high-chic, highly quotable Lindsay Lohan classic Get A Clue and celebrate Brennan's last episode before she moves halfway across the country.

Der Einstieg ist Anekdotisches aus unsrer jeweiligen Begegnung mit Christoph Schlingensief. Wir sprechen außerdem über die Bezüge zu Alexander Kluge und auch Heiner Müller kommt kurz vor. Die Filme und Theaterinstallationen Schlingensiefs werden diskutiert und vor allem, was uns damals irritiert hat und bis heute staunen lässt.

Its the end of the week for the podcast and I want to start a new trend. From now on each Thursday I'm going to offer a weekend project to complete. This could be setting up a new server or doing something IRL. Each week this is a chance to come up with something fun to do and a reason to experiment. This week my offering is to set up a self-hosted music streaming server. In my old man rant against paid streaming like Spotify, the only way out is to build my own... With blackjack and hookers. Read more Get more hot takes, deeper dives and inside information on tech and how it connects to humans. obscurednarration.com Blog RSS Get behind the scenes Twitter - @domcorriveau Instagram - @domcorriveau Reviews and more tech news on my YouTube Music "Werq", "Basic Implosion" Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/ Subscribe If a daily is just too much for you, I have a single RSS feed that compiles all the daily shows into a single audio file. This comes out each Friday and is a streamlined episode of all the daily shows combined into a single file. This way you don't miss anything but don't feel like you're falling behind! Weekly Podcast RSS Miami Vice Podcast Back in 1984, Miami Vice changed the TV landscape not only for the decade, but for generations of police drama’s since. Miami Vice’s cultural impact on TV was not only with police drama’s, but bringing in the best of popular music with each and every episode. Go With the Heat is a serial podcast going an episode at a time and digging into the cultural phenomenon that is Miami Vice from the perspective of hosts who have never seen an episode prior to starting this podcast. gowiththeheat.com

Exclusives for gaming has been a thing for a long time. Although I've been hard on walled gardens all week I'm kinda soft on gaming exclusives. In this episode I really try to convince myself gaming exclusives are bad but for me I'm already on an exclusive... Steam. Valve has given Linux gaming so much goodwill I really fight to find a problem with other exclusives. At least we got it better than OSX! Read more Get more hot takes, deeper dives and inside information on tech and how it connects to humans. obscurednarration.com Blog RSS Get behind the scenes Twitter - @domcorriveau Instagram - @domcorriveau Reviews and more tech news on my YouTube Music "Werq", "Basic Implosion" Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/ Subscribe If a daily is just too much for you, I have a single RSS feed that compiles all the daily shows into a single audio file. This comes out each Friday and is a streamlined episode of all the daily shows combined into a single file. This way you don't miss anything but don't feel like you're falling behind! Weekly Podcast RSS Miami Vice Podcast Back in 1984, Miami Vice changed the TV landscape not only for the decade, but for generations of police drama’s since. Miami Vice’s cultural impact on TV was not only with police drama’s, but bringing in the best of popular music with each and every episode. Go With the Heat is a serial podcast going an episode at a time and digging into the cultural phenomenon that is Miami Vice from the perspective of hosts who have never seen an episode prior to starting this podcast. gowiththeheat.com

You know what the absolute worst scenario is for exclusives? When both subscriptions and hardware are paired to make the ultimate pain in the ass. In this episode I express my fears of what could happen with Apple's new News+ service gets exclusives that are locked to their hardware in addition to their service. Its time I play a little conspiracy theorist! Read more Get more hot takes, deeper dives and inside information on tech and how it connects to humans. obscurednarration.com Blog RSS Get behind the scenes Twitter - @domcorriveau Instagram - ]@domcorriveau Reviews and more tech news on my YouTube Music "Werq", "Basic Implosion" Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/ Subscribe If a daily is just too much for you, I have a single RSS feed that compiles all the daily shows into a single audio file. This comes out each Friday and is a streamlined episode of all the daily shows combined into a single file. This way you don't miss anything but don't feel like you're falling behind! Weekly Podcast RSS Miami Vice Podcast Back in 1984, Miami Vice changed the TV landscape not only for the decade, but for generations of police drama’s since. Miami Vice’s cultural impact on TV was not only with police drama’s, but bringing in the best of popular music with each and every episode. Go With the Heat is a serial podcast going an episode at a time and digging into the cultural phenomenon that is Miami Vice from the perspective of hosts who have never seen an episode prior to starting this podcast. gowiththeheat.com

After SEVERAL weeks of being on a hiatus (finally wrapped the Miami Vice podcast!) I'm back with more hot takes and permanently back for these daily podcasts. To kick things off this week I spent some time over the weekend really thinking about Brian Acton's advice to delete Facebook and really look at my reliance on their apps. Naturally, this lead me to review my usage of WhatsApp and how to diversify myself away from the FAANG services. Over the weekend I took some time to set up a XMPP/Jabber server with great success! This was a fun way to experiment and I think I'm going to stick with the self-hosted chat, that was incredibly easy to set up. XMPP Server - Prosody IM Read more Get more hot takes, deeper dives and inside information on tech and how it connects to humans. obscurednarration.com Blog RSS Get behind the scenes Twitter - @domcorriveau Instagram - @domcorriveau Reviews and more tech news on my YouTube https://www.youtube.com/dominiccorriveau Music "Werq", "Basic Implosion" Kevin MacLeod (incompetech.com) Licensed under Creative Commons: By Attribution 3.0 License http://creativecommons.org/licenses/by/3.0/ Subscribe Podcast RSS Miami Vice Podcast Back in 1984, Miami Vice changed the TV landscape not only for the decade, but for generations of police drama’s since. Miami Vice’s cultural impact on TV was not only with police drama’s, but bringing in the best of popular music with each and every episode. Go With the Heat is a serial podcast going an episode at a time and digging into the cultural phenomenon that is Miami Vice from the perspective of hosts who have never seen an episode prior to starting this podcast. gowiththeheat.com

The DCTP is in the X-mas spirit!! James makes eggnog for his friends, Ryan tells us how tall he is, and everyone answers calls on the Santa Hotline in our episode all about Disney Channel's first Christmas DCOM, The Ultimate Christmas Present.

This week on the podcast, we get insight into a community network that puts extra emphasis on the word “community.” Diana Nucera, Director of the Detroit Community Technology Project (DCTP) talks with Christopher about how the people in her city and their diversity are the driving forces behind the connectivity they have created. Diana and Christopher review … Continue reading "Building Digital Equity in Detroit – Community Broadband Bits Podcast 323"

Rozmawiamy o wierze i religii w grach wideo i w ich kontekście. NOWY RSS #DCTP: http://feeds.soundcloud.com/users/soundcloud:users:335473957/sounds.rss O "Horizon:zero Dawn" rozmawiamy do ok. 31 minuty.

Rozmawiamy o motywie wiary oraz religii w grach. Zapraszamy na www.wszystkogra.tv O szczegółach fabuły Horizon: Zero Dawn kończymy mówić w okolicach 31 minuty.

Rozmawiamy o wyznaczeniu zadań i stawianiu wyzwania w grach jako elemencie narracji i funkcji rozrywkowej do czego zainspirowała nas kampania fabularna w Destiny 2. Felieton Tomka będący wprowadzeniem do odcinka: https://www.youtube.com/watch?v=nC3LXXinIdk Blog Kamila: http://grotatnik.pl/ Nasza strona: www.wszystkogra.tv

Daj Ci To Przejdę - podcast o grach wideo i komputerowych. W odcinku wspominamy o grach (Uwaga! Możliwe spoilery!): Among the Sleep Assassin’s Creed 3 Assiassins's Creed 2 Baldur’s Gate 2 Bioshock Infinite Brutal Legend Deus Ex Human Revolution Deus Ex Mankind Devided Dragon Age 2 Driver Fallout 3 Fallout New Vegas Final Fantasy XV Final Fantasy XVII Gravity Rush Gravity Rush 2 Gravity Rush Half-Life Heavy Rain Killzone 2 Killzone 3 Kingdom of Amalur Mass Effect Andromeda Mass Effect Max Payne 3 Max Paynem Metroid Other M Murderer Prey The Last of Us TitanFall 2 Tomb Raider Tomb Raider Underworld Torment Wiedźmin 2 Wiedźmin 3 Xenoblade Chronicles Zapraszamy na: - nasz fanpage: www.facebook.com/WszystkoGraTV/ - grupę Wszystko Gra: www.facebook.com/groups/616626868494911/ - serwer Discord: discord.gg/YRktsyh - stronę: www.wszystkogra.tv www.rozgrywkapodcast.pl - instagram: wszystko_gra_tv - bloga Ani: www.landminecat.pl/ - wydarzenie Kasi: www.facebook.com/events/135063713…/?__mref=message

On this season of Working, we left the East Coast behind and flew to Detroit. We’re speaking with eight people who are drawing on the city’s complex history as they work to create its future. For this episode, we spoke with Diana Nucera, director of the Detroit Community Technology Project. Diana Nucera runs an organization that promotes digital literacy and internet access in Detroit. Diana tells us about how she works to fill in technological gaps in Detroit—a city where nearly 40% of households still don't have internet access. She talks us through her organization's digital literacy programs and describes their efforts to build networks to build networks. Nucera also tells us a bit about her organization's DIY roots, and how they fit into the city's evolving political infrastructure. In a Slate Plus Extra, Nucera describes how net neutrality might change and how that might affect DCTP's work. If you’re a member, enjoy bonus segments and interview transcripts from Working, plus other great podcast exclusives. Start your two-week free trial at slate.com/workingplus. Email: working@slate.com Twitter: @Jacob_Brogan Production: Mickey Capper, @FMcapper Learn more about your ad choices. Visit megaphone.fm/adchoices

On this season of Working, we left the East Coast behind and flew to Detroit. We’re speaking with eight people who are drawing on the city’s complex history as they work to create its future. For this episode, we spoke with Diana Nucera, director of the Detroit Community Technology Project. Diana Nucera runs an organization that promotes digital literacy and internet access in Detroit. Diana tells us about how she works to fill in technological gaps in Detroit—a city where nearly 40% of households still don't have internet access. She talks us through her organization's digital literacy programs and describes their efforts to build networks to build networks. Nucera also tells us a bit about her organization's DIY roots, and how they fit into the city's evolving political infrastructure. In a Slate Plus Extra, Nucera describes how net neutrality might change and how that might affect DCTP's work. If you’re a member, enjoy bonus segments and interview transcripts from Working, plus other great podcast exclusives. Start your two-week free trial at slate.com/workingplus. Email: working@slate.com Twitter: @Jacob_Brogan Production: Mickey Capper, @FMcapper Learn more about your ad choices. Visit megaphone.fm/adchoices

Replication of the genome is strongly inhibited when high fidelity DNA polymerases encounter unrepaired DNA lesions, which can not be processed. The highly stringent active sites of these polymerases are unable to accommodate damaged bases and therefore DNA lesions block the replication fork progression. In order to overcome this problem, cells have evolved mechanisms for either repairing the damage, or synthesising past it with specially adapted polymerasases. Eukaryotic DNA polymerase eta (Pol eta), belonging to the Y-family of DNA polymerases, is outstanding in its ability to replicate through a variety of highly distorting DNA lesions such as cyclobutane pyrimidine dimers (CPDs), which are the main UV-induced lesions. Also cisplatin induced 1,2-d(GpG) adducts (Pt-GGs), which are formed in a typical cancer therapy with cisplatin can be processed by Pol eta. The bypass of such intrastrand crosslinks by high fidelity DNA polymerases is particularly difficult because two adjacent coding bases are simultaneously damaged. Thus, replication by Pol eta allows organisms to survive exposure to sunlight or, in the case of cisplatin, gives rise to resistances against cisplatin treatment. Mutations in the human POLH gene, encoding Pol eta, causes the variant form of xeroderma pigmentosum (XP V), characterized by the failure to copy through CPDs. This leads to strongly increased UV sensitivity and skin cancer predisposition. This thesis describes mechanistic investigations of the translesion synthesis (TLS) process by S. cerevisiae DNA Pol eta at atomic resolution, which were undertaken in collaboration with the Hopfner group. To study this process, cisplatin lesioned DNA had to be prepared first. Once this technique was established, the catalytic fragment of Pol eta was crystallized as ternary complex with incoming 2',3'-dideoxycytidine 5'-triphosphate (ddCTP) and an primer - template DNA containing a site specific Pt-GG adduct. The first obtained structure shows the ddCTP positioned in a loosely bound conformation in the active site, hydrogen bonded to the templating base. Realizing the importance of the 3’ hydroxy group for positioning the NTP and the DNA correctly inside the polymerase, the complex was crystallized again with a 2’-deoxynucleoside 5’-triphosphate (dNTP). To prevent nucleotidyl transfer, primer strands which terminate at the 3’-end with a 2’,3’ dideoxy ribose were prepared by reverse DNA synthesis and used for cocrystallization. The resulting crystals diffracted typically to 3.1-3.3Å resolution at a synchrotron light source. A Pol eta specific arginine (Arg73 in yeast Pol eta) was identified for its importance to position the dNTP correctly in the active site and was shown to be necessary for lesion bypass. In contrast to the fixed preorientation of the dNTP in the active site, the damaged DNA is bound flexibly in a rather open DNA binding cleft. Nucleotidyl transfer requires a revolving of the DNA, energetically driven by hydrogen bonding of the templating base to the dNTP. For the 3’dG of the Pt-GG, this step is accomplished by bona fide Watson-Crick base pairs to dCTP and is biochemically efficient and accurate. In contrast, bypass of the 5’dG of the Pt-GG is less efficient and promiscuous for dCTP and dATP. Structurally, this can be attributed to misalignment of the templating 5’dG due to the rigid Pt crosslink. In cooperation with the Cramer group the structural reasons for the blockage of RNA Polymerase II (RNAP II) by the cisplatin lesion were elucidated. Using structural as well as biochemical methods it could be shown that stalling results from a translocation barrier that prevents delivery of the lesion to the active site. AMP misincorporation occurs at the barrier and also at an abasic site, suggesting that it arises from nontemplated synthesis according to an 'A-rule' known for DNA polymerases. RNAP II can bypass a cisplatin lesion that is artificially placed beyond the translocation barrier, even in the presence of a G A mismatch. Thus, the barrier prevents transcriptional mutagenesis.

Following oxidative stress often two kinds of DNA-lesions can be found: 8-OxodG and the 2,6-Diamino-4-hydroxy-5-formamidopyrimidine (FaPydG). Both derive from the DNA-Nucleobase guanine. The FaPydG-lesion can exist both in the beta-configuration and the alpha configuration. To clarify biological questions concerning the basepairing and the encoding features of both forms it was necessary to synthesise of the alpha-anomer of the carbocyclic FaPydG-DNA-lesion. The synthesis of the beta-cFaPydG-lesion was already finished in 2005 in the Carell group. In a sixteen-step synthesis the alpha-lesion could be prepared in the form of its phosphoramidite component. Oligonucleotides could be prepared on solid support using automated DNA-synthesis. Some of the synthesised DNA-Nucleotides contained the inserted FaPydG-lesion. Others showed an oxidation to alpha-c8-OxodG. This reaction is not known for natural lesion. Just a de-hydration to guanosine under harsh conditions is described. The observations can be ex-plained by formulation of a cyclic intermediate, which is possibly oxidized by the oxidation di-lution during the DNA-synthesis. If the sequence-dependent equilibrium between alpha cFaPydG and alpha c8-Hydro,hydroxydG is at the side of the closed form, an oxidation from this interme-diate directly to 8-OxodG is imaginable. This assumption is proven by the fact that at the same time synthesised and completely identically treated DNA-strands of different sequence didn´t show oxidation products. This could be ascertained by high resolution ESI-FTICR-mesurement. With this analytical method even smallest variations are detectable in a reliable way, whereas the often used MALDI-TOF-mesurements not always show these variations. Thermodynamic studies showed that alpha-cFaPydG doesn´t form a preferred DNA-basepair. All possible basepairs showed a strong destabilisation in comparison to the undamaged, cor-rect basepair. In vitro elongation experiments with S. cerevisiae polymerase Pol eta, G. stearothermophilus DNA-polymerase I and DinB from G. stearothermophilus showed that the alpha-lesion is a definitive block for all mentioned polymerases. Only by applying rigorous re-action conditions an elongation of the primer with BstPol I could be detected. This elongation showed a favored incorporation of dCTP, followed by an incorporation of dATP. In the time to come experiments to avoid the unwanted oxidation of alpha-cFaPydG will be es-sential to promote the cocristallisation of the alpha-cFaPydG-oligomere with the Fpg-protein from Lactococcus lactis and the BstPol I. In the second part of the thesis attempts were taken to prepare tetrafunctional amino acid derivatives, which were planned to be used in a PNA-template directed ligation reaction. Liga-tion takes place between two amino acids, so that for a ligation attempt with this method there is always needed a matching pair of amino acids. This pair requires a connection of the aminoacid to the PNA over the side chain of the amino acid. The N- and C-terminus of the amino acid had to be unprotected for the ultimate ligation reaction. These termini must not react during the solid support PNA-synthesis. After ligation the template should be separated selectively. Thus, the following requirements for the pair of amino acids result: The amino acids have to possess functional side chains, which exist naturally after cleavage of the PNA-template. For the selective cleavage of the template a con-nection orthogonal to acid and base unstable protecting groups is necessary, because these are already needed for the solid support synthesis on the residual three termini of the compound. Imaginable orthogonal cleavable links are allylic compounds, which are cleavable with Pd(0), but also silicon based compounds, which are cleavable with fluoridions, would be appropriate. In the area of the Pd(0)-cleavable link a retrosynthetic cut at this funtionality should be adequate. Here, e.g. a Horner-Wadsworth-Emmons-reaction is imaginable. The aldehydes and phosphorylides needed for this key step could be prepared with good yields, however only one of the two amino acid derivatives was obtained. The synthesis of the other compound didn´t succeed under diverse reaction conditions. Also efforts to prepare analogue Pd(0)-cleavable link molecules through classic Wittig or metathese reactions with Grubbs catalysts of the first and second generation were unsuccessful. The most successful efforts to make a target molecule synthetically accessible for a first liga-tion attempt involved the preparing of a fluorid-cleavable silyl link. Here all requirements stay the same, just instead of allylic compounds silyl ethers are needed. The acid stability of the silyl protecting group depends on the size of the alkyl groups (tert-butyl>isopropyl>ethyl). Firstly, substitution experiments with the compounds Di-tert-butylchlorosilane, Di-tert-butyl-dichlorosilane and Di-tert-butylsilylbis(trifluoromethanesulfonate) were undertaken to estab-lish a connection between PNA and the amino acid through a Di-tert-butyldisilylether. How-ever, the substitutions produced just monosubstituted silanoles, irrespective of the choice of reaction conditions and substitution order. The experiments with the commercially available (3-Cyanopropyl)-diisopropyl-chlorosilane were more successfull. Here the synthesis of one of the target molecules could be achieved.