Podcasts about ecori

When reporters are working on important stories, they often use Rhode Island's Access for Public Records Act, or APRA. It gives them the ability to ask for government emails, data sets, or other documents. But APRA isn't perfect. So, there's a bill before the General Assembly to make it stronger. Ed talks with Colleen Cronin, a reporter who covers environmental issues for ecoRI, and John Marion, executive director of Common Cause Rhode Island, to learn more. Tips and ideas? Email us at rinews@globe.com.See Privacy Policy at https://art19.com/privacy and California Privacy Notice at https://art19.com/privacy#do-not-sell-my-info.

On this episode, we're joined by Colleen Cronin. Colleen is a reporter for ecoRI News, a non-profit dedicated to reporting on environmental and social justice issues in southern New England.Colleen covers rural Rhode Island. She's been with ecoRI for a year-and-a- half as part of Report for America.Colleen is a graduate of Brown University where she was editor in chief of The Brown Daily Herald. She also worked briefly at The Boston Globe.Colleen talked about her journalism origin story. She shared a story of a discussion she had with her father (a police officer) about an early piece she wrote on racial profiling and how that impacted his perspective. She also talked about her interest in environmental reporting and the kind of stories she covers on her beat, ranging from leaf peeping to the teaching of humane ways of killing fish to coverage of mass transit issues and access to public records about car crashes.And she talked about the different ways she manages her mental health, and the journalism issue most important to her.Colleen's salutes:Ivy Scott and Shannon Larson, Boston Globe The Brown Daily Herald StaffListen to Colleen talk about her reporting on ecoRI's podcast, The Blab LabThank you as always for listening. Please send us feedback to journalismsalute@gmail.com, visit our website thejournalismsalute.org and Mark's website (MarkSimonmedia.com) or tweet us at @journalismpod. And find us on TikTok at @journalismsalute.

Rodrigo Matias, diretor comercial da Ecori Energia Solar, comenta neste episódio sobre as características de uma operação e dá insights para os integradores brasileiros, além de falar sobre os bastidores do mercado de energia solar. Nos acompanhe em todas as redes sociais: Instagram: https://www.instagram.com/canal.solar/ Facebook: https://www.facebook.com/canalsolar LinkedIn: https://www.linkedin.com/company/cana... Twitter: https://twitter.com/canalsolar_ #podcast #energiasolar #integrador #setorsolar #ecori #negocios

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.14.337840v1?rss=1 Authors: D'Acunto, M. Abstract: Protein-DNA interactions play a fundamental role in all life systems. A critical issue of such interactions is given by the strategy of protein search for specific targets on DNA. The mechanisms by which the protein are able to find relatively small cognate sequences, typically 15-20 base pairs (bps) for repressors, and 4-6 bps for restriction enzymes among the millions of bp of non-specific chromosomal DNA have hardly engaged researcher for decades. Recent experimental studies have generated new insights on the basic processes of protein-DNA interactions evidencing the underlying complex dynamic phenomena involved, which combine three-dimensional and one-dimensional motion along the DNA chain. It has been demonstrated that protein molecules spend most of search time on the DNA chain with an extraordinary ability to find the target very quickly, in some cases, with two orders of magnitude faster than the diffusion limit. This unique property of protein-DNA search mechanism is known as facilitated diffusion. Several theoretical mechanisms have been suggested to describe the origin of facilitated diffusion. However, none of such models currently has the ability to fully describe the protein search strategy. In this paper, we suggest that the ability of proteins to identify consensus sequence on DNA is based on the entanglement of {pi}-{pi} electrons between DNA nucleotides and protein amino acids. The {pi}-{pi} entanglement is based on Quantum Walk (QW), through Coin-position entanglement (CPE). First, the protein identifies a dimer belonging to the consensus sequence, and localize a {pi} on such dimer, hence, the other {pi} electron scans the DNA chain until the sequence is identified. By focusing on the example of recognition of consensus sequences by EcoRV or EcoRI, we will describe the quantum features of QW on protein-DNA complexes during search strategy, such as walker quadratic spreading on a coherent superposition of different vertices and environment-supported long-time survival probability of the walker. We will employ both discrete- or continuous-time versions of QW. Biased and unbiased classical Random Walk (CRW) has been used for a long time to describe Protein-DNA search strategy. QW, the quantum version of CRW, have been widely studied for its applications in quantum information applications. In our biological application, the walker (the protein) resides at a vertex in a graph (the DNA structural topology). Differently to CRW, where the walker moves randomly, the quantum walker can hop along the edges in the graph to reach other vertices entering coherently a superposition across different vertices spreading quadratically faster than CRW analogous evidencing the typical speed up features of the QW. When applied to protein-DNA target search problem, QW gives the possibility to achieve the experimental diffusional motion of proteins over diffusion classical limits experienced along DNA chains exploiting quantum features such as CPE and long-time survival probability supported by environment. In turn, we come to the conclusion that, under quantum picture, the protein search strategy does not distinguish between one-dimensional (1D) and three-dimensional (3D) case. Copy rights belong to original authors. Visit the link for more info

NEB社の巨大な製品カタログの楽しみ方、多様な制限酵素の世界とその巧妙な仕組みについて話しました。 第123回　ポッドキャストで語るサイエンスとその魅力(実験医学) NEB…日本語のNEBウェブサイト。NEBはNew England BioLabsの略でした(BioScienceではありませんでした。)大変申し訳ございませんでした。 NEB Literature Portal…カタログのまとめページ。 NEB Catalog & Technical Reference…これがNEBが誇る400ページ超のカタログ！ Eric Lander (Wikipedia) DNA Structure and Classic experiments (Youtube)…Eric Landerの超おすすめなMITの授業動画。見よう！ 制限酵素の基礎知識 (ThermoFisher)…とてもわかり易いのでぜひ。 ファージ (Wikipedia) DNAトポイソメラーゼ (Wikipedia) ニック EcoRI…このエピソードで例として紹介しました。GAATTCという配列を認識して切断する。 回文配列(パリンドローム) DNA ligase…DNA断片同士を連結させることができる。 Gibson Assembly…配列の相補性を利用して２つ以上のDNA断片を試験管内で連結する方法。Dan Gibson博士が作ったのでみながそう呼ぶようになった。彼が自分でつけたわけではない。 DNAメチル化 Talen…ゲノム編集法のうちの一つ。 メタゲノム解析 (Online ISSN : 1881-6681) CasX enzymes comprise a distinct family of RNA-guided genome editors…地下水にすむバクテリアから単離されたCas-X オペロン (Wikipedia) IRES (Wikipedia)…Internal Ribosomal Entry Siteの略 REBASE…制限酵素のデーターベース。 IUPAC Codes - Bioinformatics.org…DNAの縮重塩基はこのように表現されます。 正規表現 Homing endonuclease I-SceI…ホーミングエンドヌクレアーゼの一種でとても長い認識配列を持つ。 I-SceI mouse model…ゲノム中にI−SceIサイトをもったマウスに対してI-SceIを発現させると二本鎖切断応答を誘導できる。この研究 (Nat. Comm 2016)など。 Miné-Hattab and Rothstein, Nat Cell Biol. (2012)…I-SceIを誘導して染色体動態の変化を観察した名論文 MmeI EcoP15I CAGE法…FANTOMの基幹技術。MmeIを用いたトリックが秀逸。 DpnI (NEB)…メチル化されたDNAのみを切断する DamID (DNA adenine methyltransferase identification) RNA splint ligase (NEB)…“SplintR LigaseはRNA splint上のDNAをライゲーションする” NEB podcast Science podcast Nature podcast iBiology デヴィッド・リンチ Ten Simple Rules to Win a Nobel Prize マシュー・メセルソン ショ糖密度勾配法…初期分子生物学の重要技術。正直やったことがないです… DNAの半保存的複製 ep7. In the golden age of molecular biology…メセルソン先生とmRNA発見の話についても触れています。 Twitter @researchat_fm Researchat.fm Editorial notes 普段あまり考えずに使っていた制限酵素について、もともとの機能や精製方法を知りとても面白かった。みんなカタログを読もう！(soh) DNA修復はすでにノーベル賞が来ていますね。メセルソン先生の偉業の数々もいつかしっかりまとめて話したい。(tadasu)

Nesta semana a jornalista Ericka Araújo conversou com o diretor-executivo da Ecori, Leandro Martins, que atua desde 2014 no setor de energia solar no Brasil. Martins analisou o mercado fotovoltaico brasileiro e compartilhou sua experiência, contando sua trajetória profissional e um case de sucesso.

Bill Bartholomew sits down with RI Rep Mike Chippendale, Eco RI environmental reporter Tim Faulkner and Burillville Land Trust's Paul Roselli for a discussion on the challenges and lack of regulatory procedures surround solar farm installation in the state.Support the show (https://www.patreon.com/bartholomewtown?fan_landing=true)

The detailed study of protein-DNA interactions is a core effort to elucidate physiological processes, including gene regulation, DNA repair and the immune response. The molecular force assay (MFA) is an established method to study DNA-binding proteins. In particular, high-affinity binder dissociation is made possible by the application of force. Microfluidic lab-on-a-chip approaches have proven helpful for parallelization, small sample volumes, reproducibility, and low cost. We report the successful combination of these two principles, forming a microfluidic molecular force assay and representing a novel use for the established MITOMI chip design. We present, characterize, validate and apply this integrated method. An alternative confocal fluorescence microscopy readout and analysis method is introduced and validated. In a multiplexing application, EcoRI binding is detected and characterized. This method paves the way for quantitative on-chip force measurements. It is suited for integration with DNA micro-spotting and in vitro expression of transcription factors to form a high-throughput chip for detailed DNA-protein interaction studies.

The major aim of this thesis was to identify novel genes of T. uilenbergi through establishment and screening of a merozoite cDNA library with the eventual goal to develop diagnostic tools using identified genes for detection of Theileria infections. The experiments were initiated by infection of sheep using T. uilenbergi stock. When parasiteamia rose, blood was collected and the merozoites were purified. Messenger RNA was isolated from purified merozoites was then utilized to establish a cDNA library. The library was titrated to be 6 x 108 pfu/ml and the recombinant clones were estimated to be 70%. Random PCR identification of the library indicated all of the inserts were of parasite origin, indicating the usefulness of the library for the identification of new genes. Random PCR amplification of inserts of the cDNA library led to the discovery of 12 single clones, among which Clone 2, 9 and 26 exhibited a high degree of identity, especially at the 3' terminus and 3' untranslated region, indicating that they belong to the same gene family. Furthermore, PCR designed to target Clone 2 amplified again four variant genes from genomic DNA of T. uilenbergi and one from genomic DNA of T. luwenshuni, suggesting this gene family is likely isolate-specific since the DNA samples for PCR were not derived from the same parasite isolate used for library construction. Sequence analysis of another genomic fragment generated with primers targeting the 3' untranslated region of the Clone 26 sequence showed that both 5' and 3' termini were highly identical to the Clone 2 gene family and these homologous termini were separated by a 136 bp sequence fragment highly identical to the 3' untranslated region of the Clone 2 gene family, indicating Clone 2 gene family members are tandemly arranged. Bioinformatic analysis of cDNA sequences of the Clone 2 gene family indicated these genes contain signal peptides and encode potential immunogenic proteins. Analysis of recombinantly expressed Clone 2 revealed immunoreactivity with sera from Theileria-infected animals from China. No cross reaction with sera of T. lestoquardi-, Babesia motasi- or Anaplasma ovis- infected animals was observed, indicating a potential specificity of this gene family. The features of the Clone 2 gene family are similar to the Tpr gene family of T. parva, which is believed to play a role in concerted evolution. Based on the highly conserved region of the Clone 2 gene family, a set of six primers were designed for the development of a loop mediated isothermal amplification (LAMP). The established assay allowed the detection of T. uilenbergi and T. luwenshuni infections simultaneously and the reaction could be simply accomplished by incubation at 63ºC for 15 min. The specificity of the reaction was confirmed through EcoRI restriction enzyme digestion analysis and sequencing. The assay was sensitive as it detected 0.1 pg DNA of T. luwenshuni or T. uilenbergi. Moreover, the assay was evaluated by testing 86 field samples in comparison to the reverse line blot method, showing a calculated sensitivity and specificity of 66.0% and 97.4%, respectively. These results indicate that the LAMP assay has a potential usefulness for application in diagnostic and pidemiological studies on T. luwenshuni and T. uilenbergi infection of small ruminants. In addition, serological screening of the library led to discovery of a positive clone called TuIP, which has been deposited in Genbank under accession number FJ467922. Partially recombinantly cloned and expressed TuIP showed strong reactivity with serum from T. uilenbergi infected animals, indicating its potential usefulness for development of novel serological diagnostic tests or serving as a candidate for vaccine development in the future.

We describe the isolation and characterization of an iridescent virus from commercially produced colonies of Gryllus bimaculatus in Germany, which showed apparent mortality. Transmission electron microscopy studies on adult cricket specimens revealed the paracrystalline assembly of icosahedral virus particles in the cytoplasm of hypertrophied abdominal fat body cells. The infecting agent could be cultivated in the lepidopteran cell line sf-9, where it caused cytopathogenic effects such as cell hypertrophy, cytoplasmic vacuolization, and cell death within 8 days postinfection. Negatively stained virus particles (n = 100) had dimensions of 172 +/- 6 nm (apex to apex) and 148 +/- 5nm (side to side). SDS-polyacrylamide gel electrophoresis of virus proteins showed more than 20 distinct polypeptides with a major species of approximately 50 kDa. Analysis of the restriction fragment length profiles from digestion of purified viral DNA with the endonucleases EcoRI, BamHI, and HindIII showed marked differences from the profiles of iridoviruses of lower vertebrates (genus Ranavirus), e.g., Rana esculenta Iridovirus and Frog virus 3. Restriction enzyme digests with the endonucleases MspI and HpaII indicated the lack of methylation of viral DNA. Polymerase chain reaction led to the amplification of a 420-bp gene fragment with 97 % sequence homology to the major capsid protein gene of Invertebrate iridescent virus 6 (IIV-6). The data indicate, that this new isolate, which is the first iridescent virus reported from Germany, belongs to the genus Iridovirus of the family Iridoviridae. It is proposed to be termed Gryllus bimaculatus iridescent virus (GbIV). The investigation of four other iridescent-like viruses which were obtained in 1998, 1999 and 2000 from various tissues of three reptile species, e.g. Pogona vitticeps, Chamaeleo quadricornis, and Chamydosaurus kingii, revealed cytoplasmic desoxyriboviruses with the exact morphological and molecular biological characteristics of the formerly described GbIV. We conclude that all five isolates of iridescent-like viruses from Germany are identical and represent variants or strains of the type species of the genus Iridovirus, IIV-6. The data indicate, that GbIV, an invertebrate iridescent virus, is able to replicate in reptiles.

Sat, 1 Jan 1994 12:00:00 +0100 http://epub.ub.uni-muenchen.de/3143/ http://epub.ub.uni-muenchen.de/3143/1/3143.pdf Webster, John A.; Bannermann, Tammy L.; Hubner, Romeo J.; Ballard, Deborah N.; Cole, Eileen M.; Bruce, James L.; Fiedler, Franz; Schubert, Karin; Kloos, Wesley Webster, John A.; Bannermann, Tammy L.; Hubner, Romeo J.; Ballard, Deborah N.; Cole, Eileen M.; Bruce, James L.; Fiedler, Franz; Schubert, Karin und Kloos, Wesley (1994): Identification of the Staphylococcus sciuri Species Group with EcoRI Fragments Containing rRNA Sequences and Description of Staphylococcus vitulus sp. nov. In: International Journal of Systematic Bacteri

Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons. Of the 16 HindIII fragments, 15 were cloned in plasmid pACYC177 in Escherichia coli HB101 (recA). The recombinant plasmid clones were characterized by cleavage with the enzymes XbaI and EcoRI. In addition, fragments generated by double digestion of cloned fragments with HindIII and XbaI were inserted into the plasmid vector pACYC184. The results obtained after hybridization of 32P-labeled cloned fragments to Southern blots of MCMV DNA cleaved with HindIII, XbaI, EcoRI, BamHI, ApaI, ClaI, EcoRV, or KpnI allowed us to construct complete physical maps of the viral DNA for the restriction endonucleases HindIII, XbaI, and EcoRI. On the basis of the cloning and mapping experiments, it was calculated that the MCMV genome spans about 235 kilobase pairs, corresponding to a molecular weight of 155,000,000. All fragments were found to be present in equimolar concentrations, and no cross-hybridization between any of the fragments was seen. We conclude that the MCMV DNA molecule consists of a long unique sequence without large terminal or internal repeat regions. Thus, the structural organization of the MCMV genome is fundamentally different from that of the human cytomegalovirus or herpes simplex virus genome.