Podcasts about high-throughput

Doing something complex and meaningful in a new way requires thinking and acting a bit differently. This is the case with how Dr. Joey Azofeifa, from Arpeggio Bio, is using systems biology to discover new drug candidates. Join us in this Season 2 kickoff episode where we dive headlong into transcriptomics, systems biology, machine learning, and learn how they're being used to innovate drug discovery. We learn about 3'-end mRNA barcoding and in-cell reverse transcription methods that allow the pooling of up to 1,536 samples so that only a single library preparation is required while still allowing the deconvolution of RNAseq results. This reduces their RNAseq costs by up to 400-fold, which enables them to generate enormous transcriptomic data sets. We also learn about how they're using generative adversarial AI networks to use this transcriptomics data to design potential drug candidates. We even hear how one of their drug candidates, which targets iron homeostasis pathways, has progress to successful testing in mice. To access the transcript for download, please visit - https://www.thermofisher.com/us/en/home/brands/invitrogen/molecular-biology-technologies/speaking-of-mol-bio-podcast.html Subscribe to get future episodes as they drop and if you like what you're hearing we hope you'll share a review or recommend the series to a colleague.  Download Transcripts: Speaking of Mol Bio Podcast | Thermo Fisher Scientific - US Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.

“A Low-Cost Automated System for High-Throughput Phenotyping of Single Oat Seeds” with James Clohessy. A Rube Goldberg machine is a machine intentionally designed to complete a simple task using overly complicated steps. James Clohessy and his team are doing just the opposite. Using machine learning, web cameras, open software, and photogrammetry techniques, they’re developing low cost, high-throughput, high efficiency phenotyping systems. With these systems, researchers can save hours of time that would normally be spent on taking individual seed measurements by hand, such as height, width, and color, all while gaining greater detail about the seed such as volume and density. Listen in to learn more about James’ new system as well as: What are phenotyping and photogrammetry? What are some of the applications of knowing seed size, color, and weight? What are some of the limitations of high-throughput phenotyping? What are some of the future applications of these machine learning systems? If you would like more information about this topic, this episode’s paper is available here: dx.doi.org/10.2135/tppj2018.07.0005 This paper is always freely available. If you would like to find transcripts for this episode or sign up for our newsletter, please visit our website: https://dl.sciencesocieties.org/publications/podcast Contact us at podcast@sciencesocieties.org or on Twitter @FieldLabEarth if you have comments, questions, or suggestions for show topics, and if you want more content like this don’t forget to subscribe. If you would like to reach out to James, you can find him here: jameswclohessy@gmail.com https://www.linkedin.com/in/jameswclohessy/ @ufifasnfrec Resources Cornell Plant Breeding and Genetics Section: https://plbrgen.cals.cornell.edu/ Paul Armstrong: https://www.ars.usda.gov/plains-area/mhk/cgahr/spieru/people/paul-armstrong/ Dr. Guo’s Easy PPC program: http://park.itc.u-tokyo.ac.jp/Field-Phenomics/ninolab/PhenotypingTools/EasyPCC.html HeatSync Labs: https://www.heatsynclabs.org/ Field, Lab, Earth is copyrighted to the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.

A successful high-throughput approach to reaction screening requires rational and systematic exploration of a broad set of variables to achieve optimization expeditiously. There are many types of potential variables that can be screened. Even with the aid of high-throughput screening tools, chances are that it’s still unlikely to screen all the possible combinations of reaction variables a process chemist desires. A well-planned high-throughput approach enables scientists to more clearly see the “big picture”, quickly abandon disadvantaged routes and rapidly pinpoint advantageous and impactful conditions to focus on. Original White Paper sponsored by Unchained Labs If you'd like to view the White Paper then follow the link below: https://www.ddw-online.com/white-papers/p321952-unleashing-high-throughput-reaction-screening-the-unchained-way.html You can also download the original White Paper pdf here: https://www.ddw-online.com/media/32/133111/(1)-may18-unchained-labs-reaction-screening.pdf For more information on Drug Discovery World, head to: https://www.ddw-online.com

CRISPR-Cas9 has rapidly transformed our ability to perform targeted gene editing. While the technique has received much interest for its potential in the field of gene therapy, advances in its high-throughput use mean it can now open up a wealth of opportunities in drug discovery too. CRISPR-Cas9, short for clustered regularly interspaced short palindromic repeats, and CRISPR-associated protein-9, has quickly established itself as an important tool for precision gene editing. This powerful technology has transformed our ability to precisely target genomic sites, proving to be faster, cheaper and more accurate than other existing genome editing methods. Original article by Dr Paul Avery and Dr Richard Massey If you'd like to view the original article then follow the link below: https://www.ddw-online.com/drug-discovery/p322103-how-high-throughput-crispr-is-set-to-deliver-knockout-performance-in-drug-discovery.html You can also download the original article pdf here: https://www.ddw-online.com/media/32/129244/(7)-how-high-throughput-crispr-is-set-to-deliver.pdf For more information on Drug Discovery World, head to: https://www.ddw-online.com

Author Alison Obergrussberger of Nanion Technologies in Munich, Germany, discusses a planar patch clamp system with modular design capable of recording up to 384 cells simultaneously, and explains how the module can be incorporated into different state-of-the-art pipetting robots for seamless integration into high-throughput screening processes.

Professor Stefan Knapp tells us how the development of chemical probes helps us to find new drugs. The role of proteins in cellular signalling and disease is best studied through the development of highly specific chemical inhibitors, which can serve as a tool molecule for functional studies. Professor Stefan Knapp works to determine the structure of protein molecules to understand their regulation and to aid the design of selective inhibitors that can be developed further into efficient drugs

Professor Stefan Knapp tells us how the development of chemical probes helps us to find new drugs. The role of proteins in cellular signalling and disease is best studied through the development of highly specific chemical inhibitors, which can serve as a tool molecule for functional studies. Professor Stefan Knapp works to determine the structure of protein molecules to understand their regulation and to aid the design of selective inhibitors that can be developed further into efficient drugs

Professor Stefan Knapp tells us how the development of chemical probes helps us to find new drugs. The role of proteins in cellular signalling and disease is best studied through the development of highly specific chemical inhibitors, which can serve as a tool molecule for functional studies. Professor Stefan Knapp works to determine the structure of protein molecules to understand their regulation and to aid the design of selective inhibitors that can be developed further into efficient drugs

The extraction of specific analytes from biological samples is a ubiquitous process spanning many areas within the life sciences. 

Dr Liz Carpenter talks about her research on membrane proteins and drug development. Membrane proteins are the gateways to our cells - with nutrients, waste products, and even DNA and proteins entering and leaving cells via these tightly controlled proteins. Drugs often target membrane proteins; therefore, understanding their molecular structure helps us design better drugs. Dr Liz Carpenter uses X-ray crystallography to solve membrane protein structures. This information is then used to improve treatments for heart disease and neurological diseases.

Dr Liz Carpenter talks about her research on membrane proteins and drug development. Membrane proteins are the gateways to our cells - with nutrients, waste products, and even DNA and proteins entering and leaving cells via these tightly controlled proteins. Drugs often target membrane proteins; therefore, understanding their molecular structure helps us design better drugs. Dr Liz Carpenter uses X-ray crystallography to solve membrane protein structures. This information is then used to improve treatments for heart disease and neurological diseases.

Dr Liz Carpenter talks about her research on membrane proteins and drug development. Membrane proteins are the gateways to our cells - with nutrients, waste products, and even DNA and proteins entering and leaving cells via these tightly controlled proteins. Drugs often target membrane proteins; therefore, understanding their molecular structure helps us design better drugs. Dr Liz Carpenter uses X-ray crystallography to solve membrane protein structures. This information is then used to improve treatments for heart disease and neurological diseases.

The application of a positive pressure based Serial Sample Loading (SSL) system to load the series of sample plugs into the capillary is demonstrated along with the adaptability of the SSL system to generate sample plugs with a variety of volumes in a predictable manner.