Podcasts about cmyc

My guests this week are not only inspiring, empowering business owners, but also my neighbors here in our Austin neighborhood! Amy Arnold and Jessica Stanley are the founders of Card My Yard. Chances are, if you've seen those fun letters in someone's yard celebrating an event, it was from Card My Yard. The two started the company in 2014, shifted to a franchise model and built their custom website in 2017, and saw exponential growth in 2020 in spite of a global pandemic.The company now has over 365 franchise owners with over 90% of those from female entrepreneurs. In 2020 alone, Card My Yard booked over 150K orders, almost 3k greetings per week company-wide. In the first nine months of 2020, average revenue per location increased to $52,831 - up from an average of $18,788 in 2019.To say this female-founded yet family-owned company is on a strong trajectory of success spurred on by a mission to serve is an understatement. In addition to supporting their franchise families and customers across the country, Card My Yard Cares (CMYC) is an extension of the brand that carries their mission even further of supporting those in need. Organizations such as Breast Cancer Research Foundation, Pop-Up Birthday, and Jail to Jobs are just some partners that CMYC has donated time and funds out of ongoing support.The StartMet at a Bible study, two moms wanting schedule flexibility and a way to contribute financially to their familiesCame up with Card My Yard as a way to celebrate kids and support their local communityCame up with logistics in their living room, bought their first lettersThe ShiftStarted adding additional locationsShifted to empower other business owners to go out and do what they did and provide for their familiesStepped back to look at how to move forward, transitioned all locations into franchise owners and it caught fire The StrategyDuring quarantine, many have been furloughed or laid off and have been able to use Card My Yard as more than what it was intended forOver 90% of Card My Yard franchisees are female entrepreneursAble to really bless their families and fill in those gaps where it's been neededThe ActionTook a leap of faith in hiring full time employeesHuge change for us in the business over the last year.Hiring infrastructure and hiring people has allowed true scaling in a sustainable wayTook counsel from advisors and other fellow business owners to be willing to share what they know about running a business.Been willing to take some risk, step out on faith The MindsetBy the end of 2021, plan is to have over 500 franchise locations across the countryTechnology - continue to reinvest in that and equip franchise owners with new toolsNew product rollouts beyond happy birthday/say anything signsKeeping it fresh and exciting for franchise owners and repeat customersSupport growth of franchise owners, hiring internally, create new resources, continue to improve infrastructure to support continued growth The Wrap-Up If you could listen to one music artist for the rest of your life, who would it be?George Strait Luke CombsWhere can people find you online?WebsiteFacebookInstagram

Stefan K. Barta, MD, of the University of Pennsylvania, joins David Henry, MD, to discuss the treatment and diagnosis of lymphoma in patients with HIV.    In this week's Clinical Correlation, Ilana Yurkiewicz, MD, has Part 2 of her discussion on informed consent in cancer. Dr. Yurkiewicz is a fellow in hematology and oncology at Stanford University and is also a columnist for Hematology News. More from Dr. Yurkiewicz here. Notes, Transcripts, Links  Show notes By Emily Bryer, DO Resident in the department of internal medicine, University of Pennsylvania.  Immunosuppression in patients with HIV, especially with low CD4 counts, is associated with the development of lymphomas.  Diffuse large B-cell lymphoma is the most common lymphoma in patients with HIV followed by Burkitt lymphoma and Hodgkin lymphoma.  Extra-nodal manifestations of lymphoma are more common in patients with HIV, especially with lower CD4 counts. Following pathologic diagnosis, staging of lymphoma should include: CT scan PET scan Evaluation of CNS (MRI brain and LP) Bone marrow biopsy Evaluation for hepatitis B and C co-infection. Fluorescence in situ hybridization (FISH) is a molecular technique that identifies portions of DNA and helps to identify translocations and rearrangements. cMYC, BCL2, and BCL6 are all pro-proliferative genes and commonly implicated in lymphoma. cMYC rearrangement pose higher risk of CNS involvement and CNS relapse.  cMYC rearrangement (as opposed to cMYC translocation) requires therapy that is more aggressive therapy than R-CHOP.  Treatment of high grade diffuse large B-cell lymphoma: R-EPOCH Ibrutinib plus R-EPOCH Resources/Links: AIDS Malignancy Consortium Blood. 2004;103:275-82. Blood. 2010 Apr 15; 115(15): 3008-16. Clinical Trial: NCT03220022  Ibrutinib, Rituximab, Etoposide, Prednisone, Vincristine Sulfate, Cyclophosphamide, and Doxorubicin Hydrochloride in Treating Patients With HIV-Positive Stage II-IV Diffuse Large B-Cell Lymphomas  

Hosts: Vincent Racaniello, Dickson Despommier, and Daniel Griffin The TWiP trio solve the case of the bug bites all in a row, and talk about a secreted Toxoplasma protein that is central to the parasite's manipulation of host cells.   Subscribe to TWiP (free) in iTunes, Google Play Music, by the RSS feed or by email Links for this episode: Pigeon mites feed on humans at night (EPA) Secreted Toxoplasma protein (mBio) Image credit Letters read on TWiP 111 This episode is sponsored by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. Case Study for TWiP 111 82 yo woman living in Washington Heights NYC 10 days of watery diarrhea, not smelly, does not float. Wakes her up at night. No fever, sweats at night. Losing some weight, appetite. No recent travel. Born in DR early 1930s, moved to US age 30. Sometimes goes back but not for several years. Springtime. Lives with extended family, only one sick. Eats whole assortment of cooked foods, rice, beans, fresh fruits. Drinks tap water. Not working. No pets. Stays mostly inside. History of reflux, high blood pressure, cognitive decline, diagnosed with temporal giant cell arteritis. Put on prednisone, doing better. Has had gall bladder removed. HIV negative. Extended family works in city, no taxi drivers. Belly uncomfortable for >week. Temp 38.1, BP 116/78, HR 105, breathing 12/min. Exam: looks ill, has diffusely tender abdomen, decreased bowel sounds. Liver, spleen not enlarged. Oriented. Has rash on abdomen: odd patchy distribution, looks like multiple thumbprints, front of belly, extending from umbilicus. White count 8, 78 polys, 14 lymphs, 4 mono, 1 eosinophil. Sodium 129, BUN 15, Creat 0.6. Urine histoplasma negative, TB test negative. Send your case diagnosis, questions and comments to twip@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVniks consider the role of a cell enzyme that removes a protein linked to the 5'-end of the picornavirus genome, and the connection between malaria, Epstein-Barr virus, and endemic Burkitt's lymphoma.   Links for this episode Divergent requirements for removing VPg (mBio) Bond, covalent bond (TWiV 210) Link between malaria and endemic Burkitt's lymphoma (PLoS Path) Multifactorial role of malaria in Burkitt's lymphoma (PLoS Path) Plasmodium infection promotes AID-dependent B cell lymphoma (Cell) Children's cancer dependent on climatic factors (Nature) Denis Burkitt (Wikipedia) Request for PACE trial data (virology blog) Letters read on TWiV 374 This episode is sponsored by 32nd Clinical Virology Symposium and ASM Grant Writing Webinar Weekly Science Picks Alan - Indoor skydivingVincent - Cancer Virus by Crawford, Johannessen, and RickinsonRich - WitKathy - The Only Woman in the Room by Eileen PollackDickson - Show everyone your clinical data Send your virology questions and comments to twiv@microbe.tv

Join us as we talk with Dr. Jay Bradner, MD of the Bradner Lab, Dana Farber Cancer Institute and Harvard Medical School. Dr. Bradner focuses his research on the use of chemical biology to target gene regulatory pathways. Specifically, Dr. Bradner is working to develop a MYC inhibitor for myeloma. MYC is the most common translocation to be found in multiple myeloma and increases in prevalance as the disease progresses, so finding a target for this translocations is critical. 

Background: Standardized and well-characterized genetic building blocks are a prerequisite for the convenient and reproducible assembly of novel genetic modules and devices. While numerous standardized parts exist for Escherichia coli, such tools are still missing for the Gram-positive model organism Bacillus subtilis. The goal of this study was to develop and thoroughly evaluate such a genetic toolbox. Results: We developed five BioBrick-compatible integrative B. subtilis vectors by deleting unnecessary parts and removing forbidden restriction sites to allow cloning in BioBrick (RFC10) standard. Three empty backbone vectors with compatible resistance markers and integration sites were generated, allowing the stable chromosomal integration and combination of up to three different devices in one strain. In addition, two integrative reporter vectors, based on the lacZ and luxABCDE cassettes, were BioBrick-adjusted, to enable beta-galactosidase and luciferase reporter assays, respectively. Four constitutive and two inducible promoters were thoroughly characterized by quantitative, time-resolved measurements. Together, these promoters cover a range of more than three orders of magnitude in promoter strength, thereby allowing a fine-tuned adjustment of cellular protein amounts. Finally, the Bacillus BioBrick Box also provides five widely used epitope tags (FLAG, His(10), cMyc, HA, StrepII), which can be translationally fused N- or C-terminally to any protein of choice. Conclusion: Our genetic toolbox contains three compatible empty integration vectors, two reporter vectors and a set of six promoters, two of them inducible. Furthermore, five different epitope tags offer convenient protein handling and detection. All parts adhere to the BioBrick standard and hence enable standardized work with B. subtilis. We believe that our well-documented and carefully evaluated Bacillus BioBrick Box represents a very useful genetic tool kit, not only for the iGEM competition but any other BioBrick-based project in B. subtilis.

This podcast summarizes and places in clinical context two studies describing straightforward immunohistochemical methods to risk-stratify diffuse large B-cell lymphoma.