Podcasts about siderophores

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.01.26.525796v1?rss=1 Authors: Kang, D., Kirienko, N. Abstract: Multidrug-resistant Pseudomonas aeruginosa is a common nosocomial respiratory pathogen that continues to threaten the lives of mechanically-ventilated patients in intensive care units and those with underlying comorbidities such as cystic fibrosis or chronic obstructive pulmonary disease. For over 20 years, studies have repeatedly demonstrated that the major siderophore pyoverdine is an important virulence factor for P. aeruginosa in invertebrate and mammalian hosts in vivo. Despite its physiological significance, an in vitro, mammalian cell culture model to characterize the impact and the molecular mechanism of pyoverdine during infection has only recently been developed. In this study, we adapt a previously-established murine macrophage-based model for human bronchial epithelial cells. We demonstrate that pyoverdine-rich conditioned medium from P. aeruginosa disrupts epithelial integrity in a manner that depends on protease activity and the type II secretion system. Disrupting pyoverdine production, whether genetically or chemically, mitigates this damage. Interestingly, this damage did not require exotoxin A or PrpL (protease IV), two previously-characterized toxins regulated by pyoverdine. We also examined the effects of exposure to purified pyoverdine on lung epithelial cells. While pyoverdine accumulates within cells, the siderophore is largely sequestered inside early endosomes, showing little cytotoxicity. This is in contrast to other, more membrane-permeable iron chelators and siderophores such as pyochelin. However, pyoverdine may indirectly contribute to lung inflammation by potentiating these iron chelators in promoting the production of proinflammatory cytokines. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

In this episode of the WSU Wheat Beat podcast, Drew Lyon interviews Dr. Tarah Sullivan about iron deficiency in wheat. RESOURCES MENTIONED: Tarah Sullivan CSS faculty page http://css.wsu.edu/tarah-s-sullivan/ Tarah's previous podcast episode http://smallgrains.wsu.edu/wsu-wheat-beat-episode-29/ Joan Davenport CSS faculty page http://css.wsu.edu/joan-davenport/ Soil & Water Resources Page http://smallgrains.wsu.edu/soil-and-water-resources/ CONTACT INFORMATION: Contact Tarah via email at t.sullivan@wsu.edu.

Hi Friends! Welcome to Season 2 of The Regenerative Agriculture Podcast! Thank you for listening, spreading the word, and helping to make this show such a hit, while expanding our community in the fast-growing regenerative agriculture movement. It is my honor to be part of this community along with you as we launch Season 2 of this show. For this season-opening episode, we are immeasurably pleased to bring Dr. Don Huber back again, sharing more of his wealth of accumulated knowledge. Don was our first guest on the show in Season 1, and you can listen to the first-ever Regenerative Agriculture Podcast episode with Don, here. Dr. Don Huber is a leading plant pathologist, Professor Emeritus at Purdue University, and prolific author, contributor, or editor of more than 300 published academic writings and three books. For more than 50 years, Don has been a renowned researcher and principal voice in the field of crop-plant pathology, especially as it relates to the overlapping spheres of mineral fertility and microbial ecology. As a research partner, consultant or advisor, he has contributed to work in more than a dozen countries and been awarded numerous honors and awards for his copious scientific contributions. Critical among these, has been his work in: nitrogen efficiency; nitrification inhibitors; plant and microbial metabolite interactions; development of aminopeptidase profiling, whereby unknown micro-organisms can be rapidly identified and then cultured, by their amino acid profile; and copious work vastly furthering the understanding of mineral/ microbe/ disease/ herbicide interaction. There are some heavy science topics in this show that may compel your own further research. But no matter what or where you grow, there are some truly high-value practicalities laid out in this conversation. This all makes him a really exciting guest to have on the show. In this episode, listen as Dr. Don Huber covers the following and more: Reducing vs. non-reducing sugars and the role they play in energy storage and metabolism. High levels of reducing sugars (glucose and fructose) are an attractant of insects and disease. Manganese is an enzyme cofactor for the sucrose phosphate synthase enzyme that converts glucose and fructose. Reducing sugars are exuded from roots, attracting pythium, Phytophthora, actinomycetes, oomycetes. Non-reducing sugars are less exuded and a poorer food source for these organisms. Most soil pathogens remain dormant and harmless until activated by external nutrient sources. The grower can control this. In disease suppressive soils, soil bacteria colonize the resting structures of dormant fungal or oomycete spores, causing fungistasis, keeping them dormant. Reducing sugars, as root exudates, disrupt this suppression by giving bacteria a more ready food source. Airborne pathogens are also attracted to a deficient nutrient profile of the above-ground plant parts. Rusts require an exogenous source of Zinc on the leaf surface for spores to germinate. Siderophores can be prevented by antibiotics, nutritional integrity, and immune responses. Some early fungicides did not affect the fungus, but rather the amino acid profile of the plant, denying the fungus its food source. Apple Scab example. Aminopeptidase profiling that Don developed. Not directly practicable for farmers, but a fascinating and now commonly used lab practice, in microbiological research. Nitrogen is not just N, from the periodic table. Ammonium, Nitrate and Amino Nitrogen must be considered as unique substances, that affect systems differently and should be used differently. Molybdenum’s role in the Nitrate Reductase enzyme functioning, and other details on nitrogen conversion and storage. Ammonium is the most efficient form of Nitrogen for corn and wheat. Nitrate requires 12-16% of all sugars just to convert to a plant usable form. Ammonia, however, cannot be stored and must be metabolized as its taken up, which can be stressful for very small plants. Efficient urea use and its reliance on the relatively abundant urease enzyme to hydrolyze ammonia. At the end of the show, Don gives this reminder: “It’s truly remarkable how everything is integrated to such a dynamic degree that it all relates together… Just look at the whole picture and make sure that we're meeting the needs of the plant… We need to look for the specific elements, but recognize that they're all part of an integrated system.” ~Don Huber For more reading of Dr. Huber’s work, view his selected bibliography and further bio. Be sure to check out the links below as I have some exciting announcements to make! Enjoy! John and The Regenerative Agriculture Podcast team at AEA   PS...  I am excited to announce that, in alignment with my mission of making regenerative agriculture the new standard for agriculture, and due to popular demand, I am opening an online academy, the Regen.Ag Academy! The first course is entitled Precision Ag Nutrition Management, offering powerful and engaging information, along with 2 CEUs upon completion. We will be launching soon so signup for our Regen.Ag Academy email list here. In the introduction section of this episode, you will hear the amazing Seth Godin (author of 18 best-selling books) mention his new Business of Food Workshop. It is worth checking out. I find a terrific amount of value in Seth's workshops. If you are a large-scale grower of corn, popcorn, pinto beans, or other grains and broadacre crops, I welcome you to attend an event I will be hosting on February 27, The Regenerative Soil and Plant Health Academy and AEA grower lunch in Garden City, Kansas. Click here to learn more. Hope to see you there!   ~   Support For This Show This show is brought to you by AEA, leaders in regenerative agriculture since 2006. If you are a large-scale grower looking to increase crop revenue and quality, email hello@advancingecoag.com or call 800-495-6603 extension 344 to be connected with a dedicated AEA crop consultant.   Sign Up For Email Updates To be alerted via email when new episodes are released, and get special updates about John speaking, teaching, and podcast LIVE recordings, be sure to sign up for The Regenerative Agriculture Podcast email list.   Feedback & Booking Please send your feedback, requests for topics or guests, or booking request have a Podcast episode recorded LIVE at your event -- to production@regenerativeagriculturepodcast.com. You can email John directly at John@regenerativeagriculturepodcast.com.   Credits This episode was Directed by Geoffrey Shively and Nathan Harman, Edited by Nathan Harman, Produced by Nathan Harman, Jenna Sodano, Robin Kitowski, and Anna Kempf.  

The Ocean Acidification Symposium was presented by the Centre for Chemical and Physical Oceanography, in November of 2012. The day-long symposium featured brief presentations from a wide range of researchers, of which this is one: Els Maas explains the vital role bacteria play in breaking down larger molecules and releasing nutrients. Bacteria are part of the microbial foodweb in the oceans.

This unit looks at the methods that have been developed by organisms for the uptake, transport and storage of iron: a process made more complicated by the insolubility of its oxides and hydroxides. You will examine iron storage in mammals, including humans, is achieved by ferritin, which stores iron as a hydrated iron (III) oxide – an example of biomineralisation. This study unit is just one of many that can be found on LearningSpace, part of OpenLearn, a collection of open educational resources from The Open University. Published in ePub 2.0.1 format, some feature such as audio, video and linked PDF are not supported by all ePub readers.