Podcasts about ns3

This week We speak With Pro Stock Driver Frank Twing With some help From Our buddy driver of the NS3 street stock Nick Sandone --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app

In this episode of Commitment Matters, Mary speaks with Erica Meyer, Owner and Publisher of October Research. Erica can be reached via email at: emeyer@octoberresearch.com During their conversation, Erica or Mary mentioned:The Title Industry has been slow to adopt technology but the pandemic has spurred it forward. What's next for Real Estate technology?The National Settlement Services Summit (NS3) is an annual gathering, hosted by October Research, of all parties in the real estate transaction to learn from each other and collaborate.The Summit features two main tracks, one focused on innovation and the other on compliance.With more than sixty presenters, Erica highlighted this year's keynote speakers: Brian Montgomery, Chairman & Founding Partner of Gate House Strategies LLC, Kathy Kraninger, VP Regulatory Affairs for Solidus Labs and Former Director of the CFPB, Gino Blefari CEO of HomeServices of America, and Stanley C. Middleman CEO of Freedom Mortgage.Mary and Erica spoke highly of the Summit's recurring Regulator Panel and the desire attending regulatory leaders have to engage with title agents in order to understand the impact of upcoming legislation. Susan Apel leads this year's session.Cannabis is still big news for the Title Industry, so one NS3 session will dive into how states are moving forward with medical and recreational allowances – and all the confusion this creates. Richard Brahmel and Art Davis lead this session.This year's Summit will include a round table, Women in Business breakfast. Topics will include work life balance, overcoming fears with confidence, and how to better support one another. Erica mentioned her love for the book Lean In Sheryl Sandberg.It's true…there are more jobs open than applicants right now, leaving title companies challenged to entice candidates into the industry.Erica mentioned the current popularity of mergers and acquisitions in our industry. Here's more on that. And, as noted, business loans are abundant right now. Here's insight from Newsweek on the topic.Cyber Security, Data Privacy and Wire fraud remain hot topics. Check out what recent podcast guest Chuck Cain offers on the matter, or our Tyler Adams episode to learn practical tactics to better protect your company from fraud.Last month, Colorado became the third state to pass comprehensive Consumer Privacy Legislation. Erica and her team vow to keep you updated as more states do the same.We can't go an episode without touching on RON and RIN. This was no exception as Erica noted the minute-by-minute updates to its surrounding legislation.Fair Lending will be much discussed at NS3 – as it is throughout the industry.We're still awaiting the confirmation of Rohit Chopra – and what the CFPB will be like once he's in office, but NS3 attendees will learn more in the closing keynote. And, podcast listeners may remember Steve Gottheim's insights on the matter.Want to submit a story or idea for October Research?If you'd like to contact the Commitment Matters podcast, email podcasts@ramquest.com. Don't forget to subscribe, rate, and review this podcast on Apple Podcast, Spotify, or wherever you listen to podcasts, or visit RamQuest.com/podcast to download the latest episode. Lastly, we love to see when and how you're listening. Share our posts, or create your own and tag them: #CommitmentMattersPodcast

Join Cloudstar's rockstar business developer Roland and CEO Greg as they hit the road to talk about TLTA, NS3 and the future of billable hours using phone systems.

Background: CTL escape mutations have been described during acute hepatitis C in patients who developed chronic disease later on. Our aim was to investigate the mutual relationship between HCV specific CD8+ T cells and evolution of the viral sequence during early acute HCV infection. Results: We sequenced multiple clones of NS3 1406 epitope in 4 HLA-A{*}02 patients with acute hepatitis C genotype 1b infection. Pentamers specific for the variants were used to monitor the corresponding CD8+ T cell response. We observed outgrowth of mutations, which induced only a weak and thus potentially insufficient CD8+ T cell response. In one patient we observed outgrowth of variant epitopes with similarities to a different genotype rather than de novo mutations most probably due to a lack of responsiveness to these likely pre-existing variants. We could show that in acute hepatitis C CTL escape mutations occur much earlier than demonstrated in previous studies. Conclusions: The adaption of the virus to a new host is characterized by a high and rapid variability in epitopes under CD8+ T cell immune pressure. This adaption takes place during the very early phase of acute infection and strikingly some sequences were reduced below the limit of detection at some time points but were detected at high frequency again at later time points. Independent of the observed variability, HCV-specific CD8+ T cell responses decline and no adaption to different or new antigens during the course of infection could be detected.

Another New Who Would Win, this time we have student vs master Rock Lee V.S Might Guy. Stay tuned more to come!

My first ultimate ninja storm generations video and includes a battle between sasuke and naruto with live commentary.

Early detection and elimination of cattle persistently infected (PI) with bovine viral diarrhoea virus (BVDV) is a key element for eradication programs. Testing dried skin biopsies derived from ear tagging might be useful for detection of BVDV in newborn calves. The aims of this study were the development of methods for antigen solubilization and RNA preparation, the investigation of the stability of these viral components and the comparison of the analytical sensitivity of different tests. Commercial antigen capture ELISAs for NS3, Erns and mixed antigens, a blocking ELISA for BVDV antibodies and two real time RT-PCR assays for 5’UTR were used as BVDV specific tests. Ear biopsies were collected from nine BVDV antibody free PI animals (infected with BVDV-I CR4043) after slaughter and from twelve PI calves (fetal infection with BVDV-I PT810) after euthanasia at the age of 5-13 days in the time of colostral antibodies. For solubilization of the BVDV antigens the detergents dodecyl sulfate, sodium deoxycholate and EMPIGEN were inappropriate. Out of the suitable detergents (CHAPS, Triton X100, Nonidet P-40, Tween 20, n-Octyl-ß-D-glucopyranoside and Digitonin) Triton X100 in a concentration of 1% was chosen for antigen solubilization. Best RNA yield was obtained using a mixer mill and a guanidine thiocyanate containing buffer, followed by RNA isolation with Qiagen RNeasy® kits (Fibrous Tissue Mini Kit and Lipid Tissue Mini Kit). RNA isolation kits provided by Roche were less efficient. NS3-ELISAs were of low analytical sensitivity for ear biopsies, maternal antibodies led to negative results. In addition NS3 epitopes are very heat sensitive. In contrast Erns-ELISAs showed high analytical sensitivity. Samples of PI animals without maternal antibodies gave mean titers above 30. Maternal antibodies had limited effects. In samples of nine PI animals with colostral antibodies the lowest titer was 13. Relevant temperatures by sample drying and storage led to minor titer reductions. The real time RT-PCR resulted in a sensitivity more than 104 fold over the detection limit, even in calves in the time of neutralizing antibodies. Bacterial or fecal contamination before sample drying had no relevant influence on the stability of Erns and 5’UTR RNA. Erns-ELISAs and real time RT-PCR seem to be suitable for detecting BVDV in dried ear notch samples of PI animals. Before appliance in BVDV eradication programs, the diagnostic sensitivity and specificity of tests using ear biopsies have to be evaluated by studies in the field with an adequate number of samples and an appropriate monitoring.

Recombinant parapoxvirus vaccine against classical swine fever: comparative characterization of immune reactions in swine Despite good efforts in eradication of classical swine fever (CSFV) within the EU massive disease outbreaks cannot be excluded. Therefore an “intervention vaccination strategy” is one option in legal EU combat strategies against CSF. At the moment an E2 subunit vaccine is licensed in the EU but possibly can be replaced by an improved vaccine to achieve optimal stimulation of the humoral as well as the cellular immune responses. The aim of the present work was the generation of a new Parapoxvirus ovis vector expressing the E2 glycoprotein of CSFV which was named ORFV D1701VrVE2. It could be successfully demonstrated that his vector vaccine efficiently expresses the foreign gene even in cells of swine as a non permissive host. It was shown to be completely avirulent for pigs. The vector is a potent stimulator of IFN-α and VSV antiviral activity in porcine PBMC supernatants. In several animal immunisation experiments with swine ORFV D1701VrVE2 induced CSFV-neutralizing serum antibodies already after a single application. Immunized piglets were protected against lethal CSFV challenge and did not develop fever. Vaccinated and challenged animals recovered from the characteristic CSFV-induced B-cell reduction. The distribution of the vaccine dose over four intra-muscular injection sites (multi-site application) led to a rapid induction of neutralizing antibodies and to solid protection from CSF after a single vaccination. In contrast to single site vaccinated animals, multi-site vaccinated piglets did not transmit the challenge virus to a naive sentinel. Two successive vector virus applications in a homologous prime-boost regimen did not provoke IFN-γ producing cells among PBMC. However, a heterologous prime-boost regimen as a combination of prime with baculovirus expressed glycoprotein E2 followed by boost with the parapoxvirus vector induced high numbers of IFN-γ producing cells. A similar beneficial effect became evident when the challenge infection mimicked the booster vaccination after a single vector prime. In contrast when the challenge CSFV was applied after a homologous prime with modified live CSFV vaccine the immunised piglets responded with lower numbers of IFN-γ producing cells as well as lower titres of CSFV-neutralizing serum antibodies. In additional experiments the adjuvant effect of lipoprotein L-OprI of Pseudomonas aeruginosa was tested in combined administration with baculovirus expressed E2 in piglets using sub-optimal doses of the E2 protein. The adjuvant showed a beneficial effect on the formation of CSFV neutralizing serum antibodies but had no influence on the number of IFN-γ producing cells in piglets before challenge. As another important CSFV subunit the immunogenic properties of the E. coli produced non structural protein NS3 have been tested. Although NS3 induced high amounts of IFN-γ producing cells in PBMC of vaccinated piglets their serum antibodies did not neutralize CSFV and even after two booster vaccinations the animals were not protected against lethal CSFV challenge.

In der vorliegenden Arbeit wurden die immunogenen Eigenschaften des Nichtstruktur-proteins 3 (NS3) des Virus der Bovinen Virusdiarrhö (BVDV) erstmals im natürlichen Wirt charakterisiert. Hierzu wurden sechs Kälber dreimal im Abstand von vier Wochen mit BVDV-NS3-rekombinantem modifiziertem Vacciniavirus Ankara (MVA) intramuskulär immunisiert (>108 infektiöse Einheiten). Vier Kälber, die mit LacZ-rekombinantem MVA (>108 infektiöse Einheiten) immunisiert wurden, bildeten die Kontrollgruppe. Fünf Wochen nach der letzten Immunisierung erfolgte eine Belastungsinfektion mit dem BVD-Virusisolat PT810 (106 kulturinfektiöse Dosen50). Für die Untersuchung der BVDV-spezifischen zellulären Immunitätsmechanismen wurden die Lymphozyten aller Tiere mit infektiösem BVDV-PT810 in vitro restimuliert und mit Hilfe verschiedener Testsysteme untersucht. Eine zytofluorometrische Nachweismethode (Fluoreszenzfarbstoff: Carboxyfluorescein Diacetate Succinimidyl Ester [CFSE]) diente der Detektion einer antigenspezifischen Lymphozytenproliferation. Die Aktivität BVDV-spezifischer zytotoxischer T-Lymphozyten (CTL) gegen autologe, BVDV-infizierte Zielzellen wurde mit Durchflusszytometrie gemessen. Mit Hilfe einer Real Time PCR wurde die Interleukin-2 (IL-2) und 4 (IL-4) mRNA-Synthese untersucht. Ein kommerziell erhältlicher ELISA (Bovigam) diente dem Nachweis der γ-Interferon-Synthese. Über einen Zeitraum von 19 Tagen nach der intranasalen BVDV-Testinfektion wurden die Kälber täglich klinisch untersucht, die Gesamtleukozytenzahl im peripheren Blut bestimmt, quantitative BVDV-Isolierungen aus den Leukozyten durchgeführt und BVDV-spezifische Antikörper mit Serumneutralisationstesten gemessen. Bereits vor der Belastungsinfektion ließ sich bei allen Kälbern, die mit BVDV-NS3-rekombinatem MVA immunisiert wurden, eine antigenspezifische Lymphozytentransformation nachweisen. Ebenso konnte bei vier Tieren der Versuchsgruppe (N = 6) nach der dritten Immunisierung eine deutliche BVDV-spezifische Zytotoxizität gegenüber autologen Hodenzellen detektiert werden. Nach der BVDV-Belastungsinfektion war die spezifische Zytotoxizität bei fünf der sechs BVDV-NS3-MVA-Impftiere höher als bei den Tieren der LacZ-Kontrolle. Im Gegensatz zur Kontrollgruppe ließ sich bei der Versuchsgruppe nach der dritten Immunisierung ein deutlicher Anstieg der IL-2 und IL-4 mRNA-Syntheserate von in vitro mit BVDV-restimulierten Lymphozyten nachweisen. Bei fünf der sechs Kälber war zu diesem Zeitpunkt auch γ-Interferon detektierbar. Der Nachweis von BVDV-neutralisierenden Antikörpern war vor der Testinfektion bei keinem Kalb möglich. Nach der Testinfektion konnte ein spezifischer Priming-Effekt für alle immunologischen Parameter, einschließlich der Bildung BVDV-neutralisierender Antikörper, festgestellt werden. Erwartungsgemäß wurden bei beiden Tiergruppen keine Krankheitssymptome beobachtet. Das Ausmaß der Leukopenie infolge der Testinfektion war gleich. Dagegen war der semiquantitativ bestimmte BVDV-Titer in Blutproben der Kontrollgruppe an den Tagen 3-13 nach der Infektion etwa doppelt so hoch (1,9-fach) wie bei der Versuchsgruppe (p = 0,016; signifikant). Im Mittel war bei der Versuchgruppe an 6,7 Tagen und bei der Kontrollgruppe an 8,0 Tagen BVDV reisolierbar (p = 0,121; nicht signifikant). Neben den beachtlichen immunologischen Stimulationseffekten wurde die Reduktion der Viruslast als Hinweis für eine protektive Wirkung des BVDV-NS3 gewertet.