Podcasts about maldi tof ms

A Pasteurella multocida episode! In this episode Luis is joined by Dr. Kelli Maddock to talk about P. multocida. Kelli was a previous guest and this time returns to talk about the capsular types of P. multocida. What are these types? What diseases are associated with them? What about hemorrhagic septicemia? Kelli also talks about a model developed to look for hemorrhagic septicemia types of P. multocida using MALDI-TOF MS. Don't miss out on a great episode!Link to Dr. Maddock's article: https://www.sciencedirect.com/science/article/pii/S0167701224001799?via%3DihubQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

If you work with MALDI-TOF MS and you read urine cultures, you probably have seen this organism : Actinotignum schaalii. Is this organism significant? What is the gram stain? Biochemicals? Other questions come up such as we do report it? Do we perform susceptibilities ? Tune in to learn about this emerging pathogen and get answers to all these questions.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

Eating the right foods can seem impossible when you're healing candida overgrowth and SIFO! The good news is, there are still great food options that can also help you heal! In this episode, I'll give you a list of 7 foods to eat every day, what foods to personalize to your needs, and what to avoid in order to fix candida overgrowth and SIFO. Watch now!  

In this episode I continue talking about MALDI-TOF MS. This time I go over the advantages and disadvantages. How has MALDI-TOF helped Clinical Microbiology? How does it compare to traditional identification methods and the workflow? What about disadvantages? Tune in to find out.

Following the webinar on MALDI-TOF MS, we think that now it is a good time to go over MALDI-TOF MS. This topic was discussed two years ago, but since there are new listeners, now is a good time to go over it again. Are you familiar with MALDI-TOF? How does it work? How does it help Clinical Microbiology? Tune in to find out.

As we enter into the holiday season, many of us look forward to celebrating long-standing traditions with family and friends, which is no different from us here on the podcast! While not necessarily as long-standing as some of the other classic holiday traditions, after 2.5 years on air, we on this podcast have established our own end-of-year tradition, which is to take a look back at some of our favorite papers or more intriguing manuscripts published in the Journal of Clinical Microbiology over the past year. And so as you'll see, thanks to the different areas of expertise and general interest among our panel today, we've selected quite a variety of papers to discuss, ranging from evaluation of new blood culture systems to use of metagenomics for infective endocarditis and to the potential application of interferon gamma release assays for detection of Histoplasma infections. And so, suffice it to say, there will be something of interest for everyone listening. But, for those watching today, you are clearly getting an extra special treat as you get to see us do this episode wearing our best holiday gear and accessories.  Guest: Dr. Trish Simner. Links: Nasal Swab Performance by Collection Timing, Procedure, and Method of Transport for Patients with SARS-CoV-2. DOI: https://doi.org/10.1128/JCM.00569-21 Multicenter Postimplementation Assessment of the Positive Predictive Value of SARS-CoV-2 Antigen-Based Point-of-Care Tests Used for Screening of Asymptomatic Continuing Care Staff. DOI: https://doi.org/10.1128/JCM.01411-21 Laboratory Safety: Handling Burkholderia pseudomallei Isolates without a Biosafety Cabinet. DOI: https://doi.org/10.1128/JCM.00424-21 The clinical utility of 2 high-throughput 16S rRNA gene sequencing workflows for taxonomic assignment of unidentifiable bacterial pathogens in MALDI-TOF MS. DOI: https://doi.org/10.1128/JCM.01769-21 Performance of Fully Automated Antimicrobial Disk Diffusion Susceptibility Testing Using Copan WASP Colibri Coupled to the Radian In-Line Carousel and Expert System. DOI: 10.1128/JCM.00777-21 Benefits Derived from Full Laboratory Automation in Microbiology: A Tale of Four Laboratories. DOI https://doi.org/10.1128/JCM.01969-20 Reflex Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of the SpeeDx ResistancePlus GC Assay. DOI: https://doi.org/10.1128/JCM.00089-21 Comparative Performance of Latest-Generation and FDA-Cleared Serology Tests for the Diagnosis of Chagas Disease. DOI: https://doi.org/10.1128/JCM.00158-21 Diagnosing Pulmonary Tuberculosis by Using Sequence-Specific Purification of Urine Cell-Free DNA. DOI: https://doi.org/10.1128/JCM.00074-21 Indeterminate QuantiFERON Gold Plus Results Reveal Deficient Interferon Gamma Responses in Severely Ill COVID-19 Patients. DOI: https://doi.org/10.1128/JCM.00811-21 Visit journals.asm.org/journal/jcm to read articles and/or submit a manuscript. Follow JCM on Twitter via @JClinMicro

David Murray, M.D., Ph.D., explains Mayo Clinic Laboratories' innovative approach to screening for plasma cell disorders and monitoring of patients being treated for multiple myeloma. This testing method, called MASS-FIX, employs matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, or MALDI-TOF MS. It offers significant advantages over traditional testing approaches and marks the first major breakthrough in multiple myeloma screening since gel electrophoresis was developed in the 1960s.

Tuberculosis is a prevalent and potentially deadly infectious disease with a worldwide incidence of more than 10 million new cases each year, and in 2015 alone, 1.4 million deaths were attributed to TB.  Much of the world relies on microbiologic techniques to detect tuberculosis including smear microscopy and mycobacterial culture which has a very long sample to answer timeframe. Both methods also have low clinical sensitivity for paucibacillary TB cases which account for more than 60% of new TB cases each year in emerging endemic areas such as China.  PCR-based methods are faster but also have limitations.  In the May 2018 issue of Clinical Chemistry, Dr. Tony Hu and his colleagues described a new peptide-based approach to detecting TB that overcomes those issues by using antibody conjugated nanoparticles, or nanodiscs, to bind specific peptides present in digested serum samples from cases of tuberculosis.  These are then detected by benchtop matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, or MALDI-TOF MS.

Initial detection and reporting by clinical microbiology laboratories is a sentinel marker for foodborne outbreak surveillance systems. Initiation of a public health investigation is reliant on the rapid initial identification of pathogens of interest. Diagnostics for Escherichia coli have evolved to reduce identification turnaround time, incorporating technologies for rapid identification (MALDI-TOF MS) and serogrouping (O157 antiserum or latex agglutination). Reporting of these isolates to a public health agency may initiate further laboratory investigations, such as pulsed-field gel electrophoresis, for confirmation that isolates may be related to a common source (clonal population).

Sat, 31 Jan 2015 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/17991/ https://edoc.ub.uni-muenchen.de/17991/1/Sauerbrey_Kerstin.pdf Sauerbrey, Kerstin

The application of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry or MALDI-TOF MS, to microbial identification is revolutionizing clinical microbiology, providing rapid identification with minimal sample preparation and potential cost savings.

Sat, 9 Feb 2013 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/15464/ https://edoc.ub.uni-muenchen.de/15464/1/Rettinger_Anna_Lena.pdf

The aim of this study was to examine whether the classical biochemical differentiation methods in relation to clostridial analysis can be replaced by newer molecular biological techniques such as real-time PCR and by MALDI-TOF MS. For this purpose, 118 potentially pathogenic Clostridium spp. isolates were compared at the Bavarian Health and Food Safety Authority using the above mentioned methods. On the basis of the obtained results a recommendation concerning the general approach to the detection of Clostridium spp. in the food industry has been elaborated.

Very recently a novel method for differentiation of bacteria and fungi has been developed, that is, identification by means of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). This differentiation relies on the exact measurement of species-specific protein spectra of ribosomal proteins. It is at least as accurate as conventional biochemical differentiation methods, but provides results within minutes. In addition to differentiation of bacteria and yeasts grown on agar plates, direct identification is feasible from positive blood cultures as well as from urine samples of patients suffering from urinary tract infections. Future developments of MALDI-TOF MS for clinical microbiological purposes include the detection of beta-lactamase and carbapenemase activity as well as genotyping of bacteria below the species level.

Background: Atopic and non-atopic wheezing may be caused by different etiologies: while eosinophils are more important in atopic asthmatic wheezers, neutrophils are predominantly found in BAL samples of young children with wheezing. Both neutrophils as well as eosinophils may secrete matrix metalloproteinase 9 (MMP-9). Considering that MMP-9 plays an important role in airway wall thickening and airway inflammation, it may influence the development of obstructive airway phenotypes in children. In the present study we investigated whether genetic variations in MMP-9 influence the development of different forms of childhood asthma. Methods: Genotyping of four HapMap derived tagging SNPs in the MMP-9 gene was performed using MALDI-TOF MS in three cross sectional study populations of German children ( age 9-11; N = 4,264) phenotyped for asthma and atopic diseases according to ISAAC standard procedures. Effects of single SNPs and haplotypes were studied using SAS 9.1.3 and Haploview. Results: SNP rs2664538 significantly increased the risk for non-atopic wheezing ( OR 2.12, 95% CI 1.40-3.21, p < 0.001) and non-atopic asthma (OR 1.66, 95% CI 1.12-2.46, p = 0.011). Furthermore, the minor allele of rs3918241 may be associated with decreased expiratory flow measurements in non-atopic children. No significant effects on the development of atopy or total serum IgE levels were observed. Conclusions: Our results have shown that homozygocity for MMP-9 variants increase the risk to develop non-atopic forms of asthma and wheezing, which may be explained by a functional role of MMP-9 in airway remodeling. These results suggest that different wheezing disorders in childhood are affected differently by genetic alterations.

Expression of aquaportin-1 in renal cell carcinoma, Hemoglobin depletion from erythrocyte cytosol for 2D gel proteomics, Serum peptide profiling using novel magnetic C18 beads offline coupled to MALDI-TOF MS, "goProteomics" website.

Expression of aquaportin-1 in renal cell carcinoma, Hemoglobin depletion from erythrocyte cytosol for 2D gel proteomics, Serum peptide profiling using novel magnetic C18 beads offline coupled to MALDI-TOF MS, "goProteomics" website.