Podcasts about escherichia coli

La dupla conformada por Alejandra Orozco y Gabriela Agúndez consiguieron su pase a la final de clavados en plataforma de 10 metros, la atleta belga Claire Michel fue hospitalizada tras la prueba del triatlón, pues aunque se aseguró que las aguas donde se llevan a cabo las pruebas están en óptimas condiciones, la joven se contagió con la bacteria Escherichia Coli. Hosted on Acast. See acast.com/privacy for more information.

La dupla conformada por Alejandra Orozco y Gabriela Agúndez consiguieron su pase a la final de clavados en plataforma de 10 metros, la atleta belga Claire Michel fue hospitalizada tras la prueba del triatlón, pues aunque se aseguró que las aguas donde se llevan a cabo las pruebas están en óptimas condiciones, la joven se contagió con la bacteria Escherichia Coli. Hosted on Acast. See acast.com/privacy for more information.

Are human brains growing larger over time, and how does that impact dementia risk? Find out about this and more in today's PeerDirect Medical News Podcast.

Les infections urinaires sont des infections localisées au niveau de la vessie. Provoquées le plus souvent par la bactérie Escherichia Coli, les infections urinaires sont plus fréquentes chez les femmes que chez les hommes. Diagnostiquées et soignées à temps, elles sont en général sans gravité. Une mauvaise prise en charge peut, en revanche, engendrer des complications, notamment pour les personnes fragiles. Quels sont les premiers signes d'une infection urinaire ? Quels conseils peut-on donner aux personnes qui souffrent d'infections urinaires à répétition ? Dr Ruxandra CALIN, infectiologue à l'hôpital Tenon APHP Paris et chercheur au sein de l'équipe « inflammation et immunité des muqueuses », à l'Institut Cochin-Institut Pasteur et au Centre d'Immunologie et des Maladies Infectieuses de Sorbonne Université.Retrouvez l'émission en entier ici :Les infections urinaires

Les infections urinaires sont des infections localisées au niveau de la vessie. Provoquées le plus souvent par la bactérie Escherichia Coli, les infections urinaires sont plus fréquentes chez les femmes que chez les hommes. Diagnostiquées et soignées à temps, elles sont en général sans gravité. Une mauvaise prise en charge peut, en revanche, engendrer des complications, notamment pour les personnes fragiles. Quels sont les symptômes de l'infection urinaire ? Quels sont les facteurs favorisant ces maladies ? Et enfin, comment prendre en charge une cystite aggravée ? Dr Ruxandra Calin, infectiologue à l'Hôpital Tenon APHP Paris et chercheur au sein de l'équipe « inflammation et immunité des muqueuses », à l'Institut Cochin-Institut Pasteur et au Centre d'Immunologie et des Maladies Infectieuses de Sorbonne Université Dr Jean Massande, chirurgien urologue andrologue, président de la Société Gabonaise d'urologie, ancien Chef du Service d'urologie du Centre Hospitalier Universitaire de Libreville. ► En début d'émission, à 100 jours du début des Jeux Olympiques de Paris-2024, nous retrouvons la chronique sport du Dr Jean Marc Sène, médecin du sport et auteur de Le sport : je me lance, aux éditions In PressToutes les chroniques sont accessibles ici.Programmation musicale :► Kokoko – Mokili ► Amber Mark – What it is.

Les infections urinaires sont des infections localisées au niveau de la vessie. Provoquées le plus souvent par la bactérie Escherichia Coli, les infections urinaires sont plus fréquentes chez les femmes que chez les hommes. Diagnostiquées et soignées à temps, elles sont en général sans gravité. Une mauvaise prise en charge peut, en revanche, engendrer des complications, notamment pour les personnes fragiles. Quels sont les symptômes de l'infection urinaire ? Quels sont les facteurs favorisant ces maladies ? Et enfin, comment prendre en charge une cystite aggravée ? Dr Ruxandra Calin, infectiologue à l'Hôpital Tenon APHP Paris et chercheur au sein de l'équipe « inflammation et immunité des muqueuses », à l'Institut Cochin-Institut Pasteur et au Centre d'Immunologie et des Maladies Infectieuses de Sorbonne Université Dr Jean Massande, chirurgien urologue andrologue, président de la Société Gabonaise d'urologie, ancien Chef du Service d'urologie du Centre Hospitalier Universitaire de Libreville. ► En début d'émission, à 100 jours du début des Jeux Olympiques de Paris-2024, nous retrouvons la chronique sport du Dr Jean Marc Sène, médecin du sport et auteur de Le sport : je me lance, aux éditions In PressToutes les chroniques sont accessibles ici.Programmation musicale :► Kokoko – Mokili ► Amber Mark – What it is.

Dr. Jessica Chen, a bioinformatician at CDC in Atlanta, and Sarah Gregory discuss E. coli O157:H7 strain linked to leafy greens-associated outbreaks.

La doctora en microbiología, profesora e investigadora de EHU, Miren Basaras, quiere completar una cartografía interior que nos muestre qué es lo que somos. Quiénes somos los seres vivos que conformamos nuestro cuerpo. Bacterias, virus y hongos comparten nuestro cuerpo. ...

La doctora en microbiología, profesora e investigadora de EHU, Miren Basaras, quiere completar una cartografía interior que nos muestre qué es lo que somos. Quiénes somos los seres vivos que conformamos nuestro cuerpo. Bacterias, virus y hongos comparten nuestro cuerpo. ...

Vous venez de boire la tasse, certes, mais la tasse d'eau de mer. Est-ce dangereux ? Est-ce une bonne idée d'en faire une habitude ?  Peut-on boire de l'eau de mer ? Alors, en petite quantité, vous ne risquez pas l'hospitalisation. Dans le cas de notre exemple, vous en avez bu une petite quantité, c'est très désagréable en bouche mais vous ne risquez rien, ou pas grand chose en tout cas. Et ce pas grand chose, c'est d'une part les micro plastiques polluants contenus dans l'eau de mer, merci les humains. Donc déjà ça, ce n'est pas jojo. Mais en plus de ça, vous profitez de la faune et la flore contenus dans cette eau de mer. Allant des algues et planctons aux bactéries, notamment Escherichia Coli, La fameuse E Coli à l'origine de troubles intestinaux et autres gastroentérites. En plus grandes quantités, imaginons que vous soyez naufragé et que vous n'avez plus que cette option. L'eau de mer contient du sel. 35gr au litre en moyenne.  Bien que les humains puissent ingérer de petites quantités de sel en toute sécurité, la teneur en sel de l'eau de mer est bien supérieure à celle qui peut être traitée par le corps humain.  De plus, lorsque nous consommons du sel dans le cadre de notre alimentation quotidienne, nous buvons également des liquides qui aident à diluer le sel et à le maintenir à un niveau sain. Les cellules vivantes dépendent du chlorure de sodium (sel) pour maintenir les équilibres et réactions chimiques du corps ; cependant, trop de sodium peut être mortel. Les reins humains ne peuvent produire qu'une urine moins salée que l'eau salée. Par conséquent, pour éliminer tout l'excès de sel absorbé en buvant de l'eau de mer, vous devez uriner plus d'eau que vous n'en avez bu. Finalement, vous mourez de déshydratation alors même que vous avez plus soif. Learn more about your ad choices. Visit megaphone.fm/adchoices

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.08.01.551406v1?rss=1 Authors: Parada, C., Yan, C.-C., Hung, C.-Y., Tu, I.-P., Hsu, C.-P., Shih, Y.-L. Abstract: The Min system contributes to the spatiotemporal regulation of division sites in Escherichia coli. The MinD and MinE proteins of this system self-organize into oscillatory waves in the form of concentration gradients. How the intracellular Min protein concentration gradients are coordinated with cell growth to achieve spatiotemporal accuracy of cell division is unknown. Here, we report that the MinD concentration gradient becomes progressively steeper as cells elongate, suggesting that the division inhibitory activity at the midcell also decreases with cell growth. Interestingly, the oscillation period appears relatively stable across different cell lengths. Similar features were found in cells under carbon stress conditions, but the gradient was even steeper, likely favoring division at shorter cell lengths. The length-dependent variation of the concentration gradient was further examined in silico using a reaction-diffusion model, which not only supported the above features, but also revealed a decrease in the midcell concentration as the shape of the gradient becomes steeper in growing cells. This growth-dependent regulation of the midcell concentration of MinD may be coupled with the FtsZ ring formation through the MinD-interacting protein MinC. We found that the variable concentration gradients occur by coordinating the reaction rates of the recruitment of MinD and MinE to the membrane and the recharging of MinD with ATP in the cytoplasm. In conclusion, this work uncovers the plasticity of MinD concentration gradients during interpolar oscillations throughout cell growth, an intrinsic property integrated during cell division. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Every chicken breeder knows this poultry type produces a significant amount of manure. It is approximately one cubic meter of waste for six months per chicken, which creates a mess in your environment and spreads unpleasant odors. Besides, fresh chicken manure can contain harmful bacteria such as Escherichia Coli and Salmonella. One of the solutions is to learn how to compost chicken manure before using it safely as an excellent addition to the soil. This procedure eliminates pathogenic microorganisms and unpleasant smells and allows you to enrich the soil with essential nutrients.  Full:  How to Compost Chicken Manure? (Step by Step Guides) 

3.22 Escherichia Coli (E. Coli) Microbiology review for the USMLE Step 1 Exam Esherichia coli (E. coli) is a lactose-fermenting, gram-negative rod causing various diseases, ranging from mild gastroenteritis to septic shock. E. coli diseases can be divided into two categories: intestinal infections and extraintestinal infections (outside the GI system). E. coli is part of the normal flora in the human GI tract, but some strains are pathogenic. Four pathogenic strains of E. coli are Entertoxigenic E. coli (ETEC), Enterohemorrhagic E. coli (EHEC), Enteroinvasive E. coli (EIEC), and Enteropathogenic E. coli (EPEC). ETEC causes watery diarrhea and is commonly found in food and water in areas with inadequate sanitation and is the most important cause of travelers' diarrhea. EHEC, also called Shiga toxin-producing E. coli (STEC), causes bloody diarrhea and is associated with contaminated food (spinach, sprouts, lettuce, fruit, undercooked beef). Shiga toxin produced by EHEC causes cell death in the GI tract and can lead to hemolytic uremic syndrome (HUS) in about 10% of cases. EIEC causes dysentery-like symptoms such as abdominal pain, fever, and bloody diarrhea. EPEC causes diarrhea, malnutrition, and growth retardation, especially in children. Extraintestinal infections include urinary tract infections (UTIs), neonatal sepsis, and pneumonia (incomplete list)  

Magic mushrooms are giving researchers hope in treating conditions like anxiety and depression, a new study may have found the key to increasing photosynthesis efficiency in plants, and research shows that we love being reached out to by old friends! Mushrooming Minds'They Broke My Mental Shackles': Could Magic Mushrooms be the Answer to Depression? by Josh Jacobshttps://www.theguardian.com/science/2019/jun/10/magic-mushrooms-treatment-depression-aztecs-psilocybin-mental-health-medicinePsilocybin Produces Substantial and Sustained Decreases in Depression and Anxiety in Patients with Life-Threatening Cancer: A Randomized Double-Blind Trial by Roland R. Griffiths, et al.https://dx.doi.org/10.1177%2F0269881116675513Rapid and Sustained Symptom Reduction Following Psilocybin Treatment for Anxiety and Depression in Patients with Life-Threatening Cancer: A Randomized Controlled Trial by Stephen Ross, et al.https://doi.org/10.1177/0269881116675512Psilocybin-Induced Decrease in Amygdala Reactivity Correlates with Enhanced Positive Mood in Healthy Volunteers by Rainer Kraehenmann, et al.https://doi.org/10.1016/j.biopsych.2014.04.010Therapeutic Mechanisms of Psilocybin: Changes in Amygdala and Prefrontal Functional Connectivity during Emotional Processing after Psilocybin for Treatment-Resistant Depression by Lea J. Mertens, et al.https://doi.org/10.1177%2F0269881119895520Limbic System: Amygdala by The University of Texas McGovern Medical Schoolhttps://nba.uth.tmc.edu/neuroscience/m/s4/chapter06.htmlSuper Plants Scientists Resurrect Ancient Enzymes To Improve Photosynthesis by Krishna Ramanujanhttps://cals.cornell.edu/news/2022/04/scientists-resurrect-ancient-enzymes-improve-photosynthesisImproving the Efficiency of Rubisco by Resurrecting Its Ancestors in the Family Solanaceae by Myat T. Lin, et al.https://doi.org/10.1126/sciadv.abm6871Scientists Take Step to Improve Crops' Photosynthesis, Yields by Krishna Ramanujanhttps://news.cornell.edu/stories/2021/08/scientists-take-step-improve-crops-photosynthesis-yieldsSmall Subunits Can Determine Enzyme Kinetics of Tobacco Rubisco Expressed in Escherichia Coli by Myat T. Lin, et al.https://doi.org/10.1038/s41477-020-00761-5Plz Call Your Friends“Unexpectedly reaching out to a friend is more appreciated than people assume, new study shows” by Douglas Heingartnerhttps://www.psychnewsdaily.com/reaching-out-more-appreciated-than-assumed/“The Surprise of Reaching Out: Appreciated More Than We Think” by Peggy J. Liu, SoYon Rim, Lauren Min, and Kate E. Minhttps://www.apa.org/pubs/journals/releases/psp-pspi0000402.pdfFollow Curiosity Daily on your favorite podcast app to get smarter with Calli and Nate — for free! Still curious? Get exclusive science shows, nature documentaries, and more real-life entertainment on discovery+! Go to https://discoveryplus.com/curiosity to start your 7-day free trial. discovery+ is currently only available for US subscribers.Find episode transcripts here: https://curiosity-daily-4e53644e.simplecast.com/episodes/mushrooming-minds-super-plants-plz-call-your-friends

I parametri del batterio Escherichia Coli nel mare della riviera emiliano-romagnola sono rientrati nei limiti in tutti i punti dove si erano registrate anomalie. Ad annunciare il ritorno alla normalità è stato l'assessore all'Ambiente dell'Emilia-Romagna, Irene Priolo, in una conferenza stampa sul tema.

Dr. Don and Professor Ben talk about the risks of plant based deli meat and soft cheeses for a pregnant woman. Dr. Don - risky ☣️ Professor Ben - risky ☣️ ‎Brief Nut Milk Cheese Risk Analysis Viability of Shiga Toxin–producing Escherichia Coli, Salmonella, and Listeria monocytogenes Within Plant Versus Beef Burgers During Cold Storage and Following Pan Frying | Journal of Food Protection Listeria Found Inside Vegan Food Plant | Food Safety News

Dr. Don and Professor Ben talk about the risks of plant based deli meat and soft cheeses for a pregnant woman.Dr. Don - risky ☣️ Professor Ben - risky ☣️ ‎Brief Nut Milk Cheese Risk Analysis Viability of Shiga Toxin–producing Escherichia Coli, Salmonella, and Listeria monocytogenes Within Plant Versus Beef Burgers During Cold Storage and Following Pan Frying | Journal of Food Protection Listeria Found Inside Vegan Food Plant | Food Safety News

S.H.U trois lettres pour Syndrome Hémolytique et Urémique, une maladie qui touche environ 160 enfants par an et qui est causée par la bactérie E. coli (Escherichia coli). Depuis le début de l'année 2022, on observe une recrudescence des cas (75 en tout dont 2 décès). En cause, certaines pizzas industrielles. Marine, le cauchemar du SHU elle l'a vécu l'été dernier, avec sa fille Augustine 3 ans, qui a été violemment touchée par ce syndrome. Docteur Cyrielle Parmentier, néphrologue pédiatre à l'hôpital Armand Trousseau à Paris fait le point avec nous sur le Syndrome Hémolytique Urémique.

In this episode, we review the high-yield topic of Escherichia coli from the Microbiology section. Follow Medbullets on social media: Facebook: www.facebook.com/medbullets Instagram: www.instagram.com/medbulletsofficial Twitter: www.twitter.com/medbulletsIn this episode --- Send in a voice message: https://anchor.fm/medbulletsstep1/message

Polo Ramírez junto a Maríajosé Soto analizaron la conferencia de prensa del presidente de Estados Unidos y Alejandra Mullet recomendó las mejores marcas para celiacos. Además, conversó con el Dr.David Montero. Investigador del Instituto de Ciencias Biomédicas (ICBM), de la Facultad de Medicina de la Universidad de Chile, sobre la inyección contra esta bacteria,

Dr. Don and Professor Ben talk about the risks from a drink where the bartender has handled lemon slices with their bare hands. Don - not risky

Dr. Don and Professor Ben talk about the risks from a drink where the bartender has handled lemon slices with their bare hands.Dr. Don - not risky

I microrganismi sono ovunque e costituiscono la stragrande maggioranza della biodiversità sul nostro pianeta

¡Bienvenido Galeno! ⚕ Te invito a que visites mi https://linktr.ee/dra.espinoza para tener acceso a mayor contenido bibliográfico y te sea de mayor utilidad. ¡ÉXITO EN TODO!

Microbiologia médica de Murray --- Send in a voice message: https://anchor.fm/las-poderosas-celulas-nk/message

Escherichia Coli Ein Kurzvortrag über das E.Coli-Bakterium von Sukadev Bretz, dem Gründer von Yoga Vidya. Hier findest du: Seminare mit Sukadev Seminarübersicht Yoga Vidya YouTube Live Kanal Online Seminare Video Seminare Yoga Vidya kostenlose App Yoga Vidya Newsletter Kochrezepte Ayurvedische Ernährung Forum Onlineshop Schon ein kleiner Beitrag kann viel bewegen... Spende an Yoga Vidya e.V.! »

Escherichia Coli Ein Kurzvortrag über das E.Coli-Bakterium von Sukadev Bretz, dem Gründer von Yoga Vidya. Hier findest du: Seminare mit Sukadev Seminarübersicht Yoga Vidya YouTube Live Kanal Online Seminare Video Seminare Yoga Vidya kostenlose App Yoga Vidya Newsletter Kochrezepte Ayurvedische Ernährung Forum Onlineshop Schon ein kleiner Beitrag kann viel bewegen... Spende an Yoga Vidya e.V.! »

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.11.11.379065v1?rss=1 Authors: Araujo, T. S., Scapin, S. M., Andrade, W., Fasciotti, M., de Magalhaes, M. T., Almeida, M. S., Lima, L. M. T. d. R. Abstract: The hydrolysis of asparagine and glutamine by L-asparaginase has been used to treat acute lymphoblastic leukemia for over four decades. Each L-asparaginase monomer has a long loop that closes over the active site upon substrate binding, acting as a lid. Here we present a comparative study two commercially available preparations of the drug containing Escherichia coli L-Asparaginase 2, performed by a comprehensive array of biophysical and biochemical approaches. We report the oligomeric landscape and conformational and dynamic plasticity of E. coli type 2 L-asparaginase (EcA2) present in two different formulations, and its relationship with L-aspartic acid, which is present in Aginasa, but not in Leuginase. EcA2 shows a composition of monomers and oligomers up to tetramers, which is mostly not altered in the presence of L-Asp. The N-terminal loop of Leuginase, which is part of the active site is flexibly disordered, but gets ordered as in Aginasa in the presence os L-Asp, while L-Glu only does so to a limited extent. Ion-mobility spectrometry mass spectrometry reveals two conformers for the monomeric EcA2, one of which can selectively bind to L-Asp and L-Glu. Aginasa has higher resistance to in vitro proteolysis than Leuginase, and this is directly related to the presence of L-Asp. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.30.362111v1?rss=1 Authors: Come, J. A. A. d. S. S., Zhuang, Y., Li, T., Brogi, S., Gemma, S., Liu, T., da Silva, E. R. Abstract: Arginase is a metalloenzyme that plays a central role in Leishmania infection. Previously, rosmarinic and caffeic acids were described as antileishmanial and as a Leishmania amazonensis arginase inhibitor and now, we describe the inhibition of arginase in L. amazonensis by rosmarinic acid analogs (1-7) and new caffeic acids derived amides (8-10). Caffeic acid esters and amides were produced by means of the engineered synthesis in E. coli and tested against L. amazonensis arginase. New amides (8-10) were biosynthesized in E. coli cultured with 2 mM of different combinations of feeding substrates. The most potent arginase inhibitors showed Ki(s) between 2 - 5.7 M. Compounds 2-4 and 7 inhibited L-ARG through a noncompetitive mechanism, and 9 showed a competitive inhibition. By applying an in silico protocol we determined the binding mode of compound 9. The competitive inhibitor of L-ARG targets key residues within the binding site of the enzyme establishing a metal coordination bond with the metal ions as well as a series of hydrophobic and polar contacts supporting its micromolar inhibition of L-ARG. These results highlight that the dihydroxycinnamic-derived compounds can be used as the basis for the development of new drugs using a powerful tool based on the biosynthesis of arginase inhibitors. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.17.343962v1?rss=1 Authors: Gong, W., Guerler, A., Zhang, C., Warner, E., Li, C., Zhang, Y. Abstract: Genome-wide protein-protein interaction determination (or interactome) remains a significantly unsolved problem in structural biology. The difficulty is twofold since high-throughput experiments (THEs) have often high false-positive rate in assigning PPIs and PPI quaternary structure is much more difficult to solve than tertiary structure using traditional structural biology techniques. We proposed a uniform pipeline to address both difficulties which recognizes PPIs by combining multi-chain threading with high-throughput experimental (HTE) data through naive Bayesian classifiers, where quaternary structures are then constructed by mapping monomer models with the dimeric frameworks through interface-specific structural alignments. The pipeline was applied to the Escherichia coli genome and created 35,125 confident PPIs that is 4.5-fold higher than HTE alone. Graphic analyses of the PPI networks reveal a scale-free cluster size distribution, which was found critical to the robustness of genome evolution and the centrality of functionally important proteins that are essential to E. coli survival. Finally, high-confidence complex structure models were constructed for all predicted PPIs built on quaternary threading templates, which show a close consistency with the experimental structures solved after the modeling. These results demonstrate the significant usefulness of threading-based homologous modeling in both genome-wide PPI network detection and complex structural construction. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.05.326769v1?rss=1 Authors: Midgett, C. R., Talbot, K. M., Day, J. L., Munson, G. P., Kull, F. J. Abstract: Infections caused by the gram-negative bacteria enterotoxigenic Escherichia coli (ETEC), Vibrio cholerae, Shigella flexneri, and Salmonella enterica are among the most common enteric pathogens and infect billions of people each year. These bacteria control expression of virulence factors using a genetic network of transcriptional regulators, some of which are modulated by small molecules as has been shown for ToxT, an AraC family member from V. cholerae. In ETEC the expression of many types of adhesive pili is dependent upon the AraC family member Rns. We present here the 3 [A] crystal structure of Rns and show it closely resembles ToxT. Furthermore, Rns contains a ligand, decanoic acid, that inhibits its activity in a manner similar to the fatty acid mediated inhibition observed for ToxT and the S. enterica homologue HilD. Rns crystallized as a dimer via an interface similar to that observed in other dimeric AraC's. Together, these results support our hypothesis that virulence controlling AraC family members are regulated by fatty acids in a common manner in a number of enteric pathogens. Furthermore, for the first time this work identifies a small molecule capable of inhibiting ETEC virulence, providing a basis for development of therapeutics against this deadly human pathogen. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.24.311373v1?rss=1 Authors: Ng, W. Abstract: Compositional changes in growth medium represents dynamic interplay between cell growth, biomass formation, and energy maintenance, with concomitant decrease in nutrients and increase in secreted metabolites and metabolic byproducts. Such information is important for quantifying microbial physiological response both at the population and cellular level, with respect to understanding subtle differences in microbial growth response, as well as supporting model building efforts in metabolic engineering. With the desire to understand molecular weight changes in components of LB Lennox medium as well as broth fractions present during cultivation of Escherichia coli DH5 (ATCC 53868) at 37 oC, 400 rpm stirring and 1 VVM aeration in a 1 L bioreactor, this study used a combination of gel filtration chromatography (GFC) and reversed phase high performance liquid chromatography (RP-HPLC) for determining changes to molecular weight of different fractions of the growth medium. Experiment results revealed the difficulty of fractionating the culture broth with RP-HPLC, where no distinct peaks of narrow retention time width were obtained. More importantly, the column used for GFC was unable to differentiate small molecular weight changes on the order of a few tens to few hundred Da through a refractive index detector. Together, GFC and RP-HPLC highlighted the difficulty of fractionating LB Lennox culture broth into different distinct fractions. Finally, the study validated the use of 194 nm as detection wavelength for visualizing the chromatogram of LB Lennox medium eluted from a C-18 reversed phase column during liquid chromatography. Collectively, GFC and RP-HPLC could not fractionate LB Lennox broth of E. coli DH5 into distinct fractions for further analysis by identification techniques such as mass spectrometry. Given the inherent complexity of complex medium such as LB Lennox, clean separation of the medium into every component with high purity may be impossible. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.09.18.303305v1?rss=1 Authors: Semeraro, E. F., Marx, L., Mandl, J., Frewein, M., Scott, H., Prevost, S., Bergler, H., Lohner, K., Pabst, G. Abstract: We have revised a previously reported multi-scale model for (ultra) small angle X-ray (USAXS/SAXS) and (very) small angle neutron scattering (VSANS/SANS) of live Escherichia coli based on compositional/metabolomic and ultrastructural constraints. The cellular body is modelled, as previously described, by an ellipsoid with multiple shells. However, scattering originating from flagella was substituted by a term accounting for the oligosaccharide cores of the lipopolysaccharide leaflet of the outer membrane including its cross-term with the cellular body. This was mainly motivated by (U)SAXS experiments showing indistinguishable scattering for bacteria in the presence and absence of flagella or fimbrae. The revised model succeeded in fitting USAXS/SAXS and differently contrasted VSANS/SANS data of E. coli ATCC 25922 over four orders of magnitude in length scale, providing specifically detailed insight into structural features of the cellular envelope, including the distance of the inner and outer membranes, as well as the scattering length densities of all bacterial compartments. Consecutively, the model was also successfully applied to E. coli K12, used for our original modelling, as well as for two other E. coli strains, detecting significant differences between the different strains in terms of bacterial size, intermembrane distance and its positional fluctuations.These findings corroborate the general applicability of our approach to quantitatively study the effect of bactericidal compounds on ultrastructural features of Gram-negative bacteria without the need to resort to any invasive staining or labelling agents. Copy rights belong to original authors. Visit the link for more info

This episode brought to you by Chipotle.Theme music is "Lovesick" written and performed by Mors Ontologica.

This episode brought to you by Chipotle.Theme music is "Lovesick" written and performed by Mors Ontologica.

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.27.258715v1?rss=1 Authors: Haslinger, K., Hackl, T., Prather, K. L. Abstract: O-methyltransferases are ubiquitous enzymes involved in biosynthetic pathways for secondary metabolites such as bacterial antibiotics, human catecholamine neurotransmitters, and plant phenylpropanoids. While thousands of putative O-methyltransferases are found in sequence databases, few examples are functionally characterized. From a pathway engineering perspective, however, it is crucial to know the substrate and product ranges of the respective enzymes to fully exploit their catalytic power. In this study, we developed an in vitro prototyping workflow that allowed us to screen ~30 enzymes against five substrates in three days with high reproducibility. We combined in vitro transcription/translation of the genes of interest with a microliter-scale enzymatic assay in 96-well plates. The substrate conversion was indirectly measured by quantifying the consumption of the S-adenosyl-L-methionine co-factor by time-resolved fluorescence resonance energy transfer rather than time-consuming product analysis by chromatography. This workflow allowed us to rapidly prototype thus-far uncharacterized O-methyltransferases for future use as biocatalysts. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.03.235192v1?rss=1 Authors: Gifford, I., Dasgupta, A., Barrick, J. E. Abstract: Due to their universal presence and high sequence conservation, rRNA sequences are used widely in phylogenetics for inferring evolutionary relationships between microbes and in metagenomics for analyzing the composition of microbial communities. Most microbial genomes encode multiple copies of ribosomal RNA (rRNA) genes to supply cells with sufficient capacity for protein synthesis. These copies typically undergo concerted evolution that keeps their sequences identical, or nearly so, due to gene conversion, a type of intragenomic recombination that changes one copy of a homologous sequence to exactly match another. Widely varying rates of rRNA gene conversion have previously been estimated by comparative genomics methods and using genetic reporter assays. To more directly measure rates of rRNA intragenomic recombination, we sequenced the seven Escherichia coli rRNA operons in 15 lineages of cells that were evolved for ~13,750 generations with frequent single-cell bottlenecks that reduce the effects of selection. We identified 34 gene conversion events and estimate an overall rate of intragenomic recombination events between rRNA copies of 3.2 x 10-4 per generation or 5.3 x 10-5 per potential donor sequence. This rate varied only slightly from random expectations between different portions of the rRNA genes and between rRNA operons located at different locations in the genome. This accurate estimate of the rate of rRNA gene conversions fills a gap in our quantitative understanding of how ribosomal sequences and other multicopy elements diversify and homogenize during microbial genome evolution. Copy rights belong to original authors. Visit the link for more info

Gram negative bacilli - Escherichia coli

Un batterio di Escherichia Coli è stato creato in laboratorio sintetizzandone completamente il DNA. --- Cambridge scientists create world’s first living organism with fully redesigned DNA - The Guardian

"Imperativer Harndrang" ist die deutlich interessantere und wissenschaftliche Bezeichnung, wenn sich unzählige Male am Tag unsere Blase meldet. Normalerweise entleert sich unser Hohlorgan Blase 5-6-mal am Tag, es sei denn, es haben sich Bakterien eingeschlichen, die eine Blasenentzündung verursachen. Alles fängt an mit einem Stechen, Ziehen und dem ständigen Drang aufs Klo zu gehen. Hollywood Schauspieler Tom Hanks sagte zurecht in seinem Film The Green Mile "es ist als müsste ich Rasierklingen pissen", als er an chronischer Blasenentzündung litt. Klingt lustig, aber als Betroffener wünscht man sich nichts mehr als eine schnelle Genesung. So gilt es Escherichia Coli, oder Enterokokken Bakterium, welches meist der Hauptgrund für eine Harnwegs- oder Blasenentzündung ist, schnell den Garaus zu machen. Nicht immer sind Antibiotika notwendig. In leichten, unkomplizierten Fällen können Haus- oder Schmerzmittel genügen. Was wir auf jeden Fall über diese lästige Irritation unserer Blase wissen sollten, berichtet uns die erfahrene Dr. Anja Herold. Sie ist Urologin in Berlin Charlottenburg und zu Gast im Vita Talk mit Antje Radünz. Und falls es unterwegs mal wieder ganz schnell gehen muss hier ein kleiner Tipp to go: Mit "Toilet Finder" finden Sie schnell ein WC in Ihrer Nähe. Die App hat mehr als 150.000 stille Örtchen im Repertoire weltweit.

On the Dr. Patrick Flynn show Ross and Dr. Patrick talk about the burning topic of UTI's and how a simple sugar compound, known as D-Mannose, combats the main bacteria knows as  Escherichia Coli or simply 'E Coli' for short. Send your questions to: askdrpatrick@twwclinics.com Find a clinic: https://twwclinics.com/clinics/ Find a lab test: http://www.twwlabs.com/

Betrachtungen und Reflektionen zu Escherichia Coli. Simple und komplexe Gedankengänge über Escherichia Coli in diesem Audio Podcast. Eine Ausgabe des Naturheilkunde Podcasts von und mit Sukadev Bretz, Yogalehrer bei Yoga Vidya. Anmerkung: Gesundheitliche Informationen in diesem Podcast sind nicht gedacht für Selbstdiagnose und Selbstbehandlung, sondern Gedankenanstöße aus dem Gebiet der Naturheilkunde. Bei eigener Erkrankung brauchst … „Escherichia Coli“ weiterlesen

Show notes so you can follow along at home: * [Sonny Perdue](https://en.wikipedia.org/wiki/Sonny_Perdue) * [CRISPR](https://en.wikipedia.org/wiki/CRISPR) * [Dr. Richard Linton](https://norocore.ncsu.edu/about/our-team/dr-richard-linton/) * [USDA, Risk Assessment & Cost-Benefit Analysis](https://www.usda.gov/oce/risk_assessment/forums.htm) * [Agricultural Communications Major, Auburn University](http://bulletin.auburn.edu/undergraduate/collegeofagriculture/agcommunications_major/) * [FSIS Mass Media Campaign Plan](https://www.fsis.usda.gov/wps/wcm/connect/8f313f7f-57bc-465b-b78d-c8204db01e48/BFS_Theoretical_Framework.pdf?MOD=AJPERES) * [Loitering](http://www.dictionary.com/browse/loitering) * [Fortunate Son, Do By Friday podcast](http://dobyfriday.com/45) * [How to Make Mind-Blowingly Delicious Burgers with Sous Vide - YouTube](https://www.youtube.com/watch?v=sIoo3TqKe-M) * [Food Safety Talk 85: I'm the jerky police (with Dan Benjamin)](http://foodsafetytalk.com/food-safety-talk/2015/12/18/food-safety-talk-85-im-the-jerky-police) * [Food Safety Talk 79: You’re Into Botulism Country (with Merlin Mann)](http://foodsafetytalk.com/food-safety-talk/2015/8/6/food-safety-talk-79-youre-into-botulism-country-with-merlin-mann) * [Death Wish Nitro: Safety Recall – Death Wish Coffee Company](https://www.deathwishcoffee.com/pages/important-safety-notice-recall-death-wish-nitro) * [A Weeknight Meal That Needs No Introduction - The New York Times](https://www.nytimes.com/2017/09/22/dining/sausage-peppers-recipe.html?&_r=0) * [Ewen Todd](http://ewentoddconsulting.com/2.html) * [Food Waste : ReFED | Rethink Food Waste](http://www.refed.com/?sort=economic-value-per-ton) * [Debra Palmer, Nutritional Sciences at Rutgers SEBS)](http://nutrition.rutgers.edu/faculty/debra-palmer.html) * [Transfer of Escherichia Coli to Lemons Slices and Ice during Handling](http://www.ccsenet.org/journal/index.php/jfr/article/view/69114/37663) * [Canadian Center of Science and Education FAQs](http://web.ccsenet.org/faqs.html) * [Multistate Outbreak of Multidrug-Resistant Campylobacter Infections Linked to Contact with Pet Store Puppies](https://www.cdc.gov/campylobacter/outbreaks/puppies-9-17/index.html) * [Trendy beer names, invented by neural network](http://lewisandquark.tumblr.com/post/166075083502/trendy-beer-names-invented-by-neural-network) * [Don’t use a neural network to name your next pub](http://lewisandquark.tumblr.com/post/165081046962/dont-use-a-neural-network-to-name-your-next-pub)

Initial detection and reporting by clinical microbiology laboratories is a sentinel marker for foodborne outbreak surveillance systems. Initiation of a public health investigation is reliant on the rapid initial identification of pathogens of interest. Diagnostics for Escherichia coli have evolved to reduce identification turnaround time, incorporating technologies for rapid identification (MALDI-TOF MS) and serogrouping (O157 antiserum or latex agglutination). Reporting of these isolates to a public health agency may initiate further laboratory investigations, such as pulsed-field gel electrophoresis, for confirmation that isolates may be related to a common source (clonal population).

Fri, 11 Mar 2016 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/19244/ https://edoc.ub.uni-muenchen.de/19244/1/Meyer_Christian.pdf Meyer, Christian

Sat, 18 Jul 2015 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/18586/ https://edoc.ub.uni-muenchen.de/18586/1/Gerhofer_Carolin_E.pdf Gerhofer, Carolin

Das Einkomponentensystem CadC in Escherichia coli zählt zur Gruppe der ToxR-ähnlichen Transkriptionsregulatoren und aktiviert bei niedrigem pH-Wert die Expression des cadBA-Operons, einem Säure-induzierbaren Lysin-Decarboxylase-System. Transkriptionsregulatoren der ToxR-Familie zeichnen sich durch einen gemeinsamen modularen Aufbau aus und bestehen aus einer periplasmatischen Sensordomäne, einer Transmembranhelix und einer zytoplasmatischen Effektordomäne. Die Signalwahrnehmung, -weiterleitung und -verarbeitung erfolgt bei den ToxR-ähnlichen Transkriptionsregulatoren innerhalb eines einzelnen Proteins. Die molekularen Mechanismen der Reizwahrnehmung durch CadC sind bekannt, die Signalweiterleitung und -verarbeitung im Zytoplasma sind hingegen weitgehend ungeklärt. In CadC ist ein zytoplasmatischer Linker (51 Aminosäuren) essentiell für die Signaltransduktion von der sensorischen Domäne zur DNA-Bindedomäne. Im ersten Teil dieser Arbeit wurde der Mechanismus der Signalweiterleitung von der sensorischen Domäne zur DNA-Bindedomäne untersucht. Mit Hilfe der Kernspinresonanzspektroskopie konnte gezeigt werden, dass die Linkerregion unstrukturiert vorliegt. Im Rahmen einer umfangreichen Mutagenesestudie wurde beobachtet, dass sowohl eine Vielzahl an Aminosäuresubstitutionen (Veränderungen der Ladung, der Rigidität oder der Wahrscheinlichkeit zur Bildung einer α-Helix) als auch die Verlängerung des CadC-Linkers zu keiner funktionellen Beeinträchtigung führte. Jedoch wurde die Signalverarbeitung im Zytoplasma durch Verkürzung des Linkers modifiziert und verursachte ein invertiertes Expressionsprofil des Zieloperons cadBA oder die Entkopplung der Expression vom externen pH. Der Linkerregion in CadC konnte keine Rolle in der Oligomerisierung zugeordnet werden. Unabhängig vom Linker wurde in einer in vivo Interaktionsstudie eine pH-abhängige Interaktion (pH < 6,8) zwischen CadC-Monomeren gezeigt. Im zweiten Teil dieser Arbeit wurde die Röntgenkristallstruktur (2,0 Ångström) und in einem parallelen Ansatz die NMR-Struktur (0,46 backbone RMSD) der zytoplasmatischen Effektordomäne in CadC als erste dreidimensionale Struktur der DNA-Bindedomäne eines ToxR-ähnlichen Regulators aufgeklärt. In der Struktur von CadC1-107 wurde ein „winged Helix-Turn-Helix“-Motiv aus der Familie der OmpR-ähnlichen Transkriptionsregulatoren beobachtet. Im Gegensatz zu der Topologie bereits gelöster OmpR-ähnlichen Regulatoren enthält CadC am Übergang von DNA-Bindedomäne und Linkerregion einen zusätzlichen β-Strang (β-Strang 7), welcher sich stabilisierend auf die DNA-Bindung auswirken könnte. Im dritten Teil dieser Arbeit wurde der DNA-Bindemechanismus von CadC an den cadBA-Promotor untersucht. In in vitro Versuchen zur Bindung von löslichen CadC-Varianten an DNA konnte eine sehr geringe Dissoziationsrate beobachtet werden. Somit ist nicht die Affinität zur DNA sondern die Stimulus-abhängige Interaktion von CadC mit der α-Untereinheit der RNA-Polymerase essentiell für die Aktivierung des cadBA-Operons. Außerdem wurden, basierend auf der Kristallstruktur der DNA-Bindedomäne von CadC Aminosäuresubstitutionen durchgeführt. Die Aminosäure His66 in der Erkennungshelix α3 ist an der Interaktion mit der großen Furche der DNA beteiligt, während die Aminosäuren Lys95 und Arg96 die Interaktion mit der kleinen Furche der DNA vermitteln. Die Ergebnisse dieser Arbeit postulieren ein Modell zur Signalverarbeitung in CadC, in welchem die Signalwahrnehmung im Periplasma zu konformationellen Veränderungen des unstrukturierten CadC-Linkers führt und somit die räumliche Positionierung der DNA-Bindedomänen im CadC-Dimer ermöglicht wird.

* ¿Conspiranoia? ¿Quiénes son los Illuminati? ¿Están subordinados a entidades no humanas, no físicas, con distinto nivel de evolución? ¿Una enfermedad espiritual en la razón de ser de los Illuminati? * Fundación Bill and Melinda Gates compra acciones de Monsanto (Agente Naranja; PCB). ADN de la Escherichia Coli en los alimentos. El ejército privado de Monsanto. * Planet Parenthood y la... eugenesia. Monsanto y Greenpeace. ¿Por qué Greenpeace no afecta a ciertos países? Una inversión incoherente en Shell. La World Wildlife Fund (WWF). El Club de Roma. Guerra en África. El negocio de la venta de antigripal para la falsa pandemia de Gripe A. * Podrás encontrar los enlaces relacionados en la entrada correspondiente de nuestro sitio web: https://alfilodelarealidad.com/