Let's Talk Micro

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Clinical Microbiology explained in simple terms, suitable for students and microbiologists alike. Hosted by Luis Plaza, a Medical Laboratory Scientist, he also talks about his experiences as a clinical microbiologist.

Luis Plaza

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    • May 29, 2025 LATEST EPISODE
    • weekly NEW EPISODES
    • 33m AVG DURATION
    • 197 EPISODES


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    Latest episodes from Let's Talk Micro

    190: Aztreonam-avibactam

    Play Episode Listen Later May 29, 2025 34:50


    This week Luis is joined by Dr. Callan Bleick and Dr. Mohammed Al Musawa to talk about an antibiotic that was recently approved by the Food and Drug Administration (FDA): aztreonam-avibactam.  Our guests wrote a review about this drug and they joined the podcast to talk about it. What is aztreonam? What is avibactam? Why combine them? What type of activity has it demonstrated against beta-lactamase producing organisms? When will it be available? Tune in for more information.   Link to review:    https://accpjournals.onlinelibrary.wiley.com/doi/10.1002/phar.4629   Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    189: Outbreak Detection System

    Play Episode Listen Later May 22, 2025 34:31


    There are many challenges when it comes to outbreak detection. It involves surveillance, time, and effort among other factors. Some of these challenges are linking multiple patients to an outbreak, or investigating a potential outbreak that turns out to be separate occurrences of an infection. In this episode Luis is joined by Dr. Alex Sundermann to talk about an outbreak detection system developed by his team at the University of Pittsburgh Medical Center. What components did they use to develop this system? What were the major outcomes? Is there a potential for laboratories to develop a system like this one?   Link to article: https://doi.org/10.1093/cid/ciaf216 Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    188: Haemophilus: ALA, susceptibilities, and more

    Play Episode Listen Later May 15, 2025 25:27


    This week Luis continues going over Haemophilus. This time he goes over biochemicals, testing systems, and susceptibility testing. What is the ALA test? What commercial systems are able to identify Haemophilus? What about for Haemophilus influenzae? What is HTM agar? Are there any vaccines for H. influenzae? Tune in to find out more. More information on  H. influenzae vaccines:  https://www.cdc.gov/hi-disease/vaccines/index.html   Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    187: Haemophilus

    Play Episode Listen Later May 8, 2025 26:53


    In this episode Luis continues going over organisms. This time he goes over the genus Haemophilus. We start with an overview of the different species of Haemophilus. Why can't Haemophilus grow on blood agar? What are the X and V factors? Satellite phenomenon? What is the gram stain? What about media? Tune in for the first part of a series about Haemophilus.   Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    186: Talking CPEP Fellowship

    Play Episode Listen Later May 1, 2025 51:27


    If you are part of the clinical microbiology community, you might have heard of a CPEP Fellowship, or might have met someone pursuing one. In this episode Luis is joined by Dr. Samuel Goodfellow to talk about the  Subcommittee on Postgraduate Educational Programs (CPEP) Fellowship. Dr. Goodfellow is currently participating in one and he joins us to go over the different components of it. What is a CPEP Fellowship? What is the application process like? What are the daily activities of a fellow? What kind of training does it involve? Tune in to learn about  this program and how it prepares you for your career in clinical microbiology. Information on CPEP Fellowships: https://asm.org/fellowships/cpep Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    185: The Path to Microbiology: their journey pt. 3

    Play Episode Listen Later Apr 24, 2025 80:29


    What led or inspired you to choose the field of microbiology or laboratory medicine? Has your profession changed within the field? In this series where microbiologists and people from laboratory medicine talk about their careers, Luis is joined by Dr. Andrea Prinzi and Lisa Parlich. Andrea is a microbiologist that works in the industry and the co-host of the AMR series in this podcast. Lisa is a microbiologist who works now as an Infection Preventionist.  What was their start in their lab like? What challenges did they encounter along the way? What led them to choose their profession? Tune in for another great episode.    Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    184: Lariocidin

    Play Episode Listen Later Apr 17, 2025 35:48


    Discovery of new potential antimicrobial compounds is important as we take many measures to combat antimicrobial resistance (AMR). In this episode Luis is joined by Dr. Manoj Jangra and Dr. Dmitrii Travin to discuss lariocidin: a potential antibiotic. What is the mechanism of action? What are lasso peptides? Tune in to listen about the discovery of this potential antibiotic, organisms it has shown activity against, challenges, and more.    Link to article: https://www.nature.com/articles/s41586-025-08723-7   Questions? Feedback? Send those to letstalkmicro@outlook.com   Want to support the podcast? Here's how:   Venmo: https://venmo.com/u/letstalkmicro   Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    183: 101 Topics for Clinical Microbiology Lab Leaders

    Play Episode Listen Later Apr 10, 2025 30:51


    There are many challenges in the microbiology laboratory. As a leader, you have to deal with things like validations, verifications, and knowledge of regulations among other things. As a microbiologist in a non-leadership position you need to be familiar with some of these as well. With so much information out there, where do we go? In this episode Luis is joined by Dr. Rebekah Martin, author of the “101 Topics for Clinical Microbiology Laboratory Leaders” book. This book was made in order to bring all of this information to one place. In the episode Dr. Martin discussed the process of writing the book, what type of content it has, and more.  Link to purchase the book:  https://www.wiley.com/en-us/101+Topics+for+Clinical+Microbiology+Laboratory+Leaders%3A+Accreditation%2C+Verification%2C+Quality+Systems%2C+and+More-p-9781683674474   Questions? Feedback? Send those to letstalkmicro@outlook.com   Want to support the podcast? Here's how:   Venmo: https://venmo.com/u/letstalkmicro   Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

    182: TBT- Next Generation Sequencing

    Play Episode Listen Later Apr 3, 2025 47:29


    The field of Microbiology continues to change. Organisms were primarily identified in the clinical laboratory by biochemical testing. Then MALDI-TOF came to the lab and was incorporated into the workflow. Now labs are bringing next-generation sequencing (NGS). What is NGS? How does it work? Have you heard of Nanopore technologies? In this episode Dr. Jose Alexander and Daniel Navas from Advent Health Orlando, join the podcast to talk about NGS. They brought it to their laboratory and discuss the whole process, including the validation, challenges, and more. This episode was originally published on February 9th, 2024 Guest: Dr. Jose Alexander Daniel Navas Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

    181: Microbiology CAP Checklists: Latest Updates

    Play Episode Listen Later Mar 27, 2025 26:02


    Is your laboratory accredited by the College of American Pathologists (CAP) ? Are you familiar with their checklists? What are the latest updates to the microbiology checklist? In this episode Luis is joined by Dr. Christi Wojewoda to discuss the latest updates to the microbiology checklist: media quality control and nomenclature.  Why are some media exempt and some non-exempt? Regarding nomenclature, do we need to report the new name or the old name? Tune in to find out.   ASM IQCP template link: https://asm.org/protocols/individualized-quality-control-plan-iqcp   Questions regarding checklists? Contact CAP at accred@cap.org Questions? Feedback? Send those to letstalkmicro@outlook.com Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

    CLSI M100-Ed35: updates and more

    Play Episode Listen Later Mar 20, 2025 42:06


    The latest edition of the CLSI M100 has been published. What are the latest updates? One of them is the deletion of breakpoints for Burkholderia cepacia complex. What are the recommendations for susceptibility testing? Dr. April Bobenchik returns to the podcast to talk to Luis about the latest updates. She also talks about the M45. Will there be an update to the M45? Tune in to find out. Link to CLSI free resources: https://clsi.org/all-free-resources/Link to webinar: https://clsi.org/standards/products/microbiology/documents/m100/Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    179: Eikenella

    Play Episode Listen Later Mar 13, 2025 25:41


    This week Luis continues going over organisms. This time he goes over Eikenella. Are there any other Eikenella species other than Eikenella corrodens? What is the morphology of E. corrodens? How about biochemicals? What are the members of the HACEK group? What is the HACEK group known for? Tune in to learn about this organism, which is seen in infections such as bite wounds, periodontitis, endocarditis, and more. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    178: Talking Micro with Janet Hindler

    Play Episode Listen Later Mar 6, 2025 45:46


    Talk about a great career! In this episode Luis is joined by Janet Hindler. Janet is a microbiologist with a career that has spanned over many decades. Just like most Medical Laboratory Scientists, she started working in the lab and early on she had a passion for microbiology. Then she got involved in work that took her beyond the bench such as the Clinical Laboratory Standards Institute (CLSI) and the Center for Disease Control and Prevention (CDC). In this episode Janet talks about her career and offers her opinions on mentorship and the value of networking. Tune in for a great episode!Link to ASM's biographical feature: https://journals.asm.org/doi/10.1128/jcm.00511-22Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    177: MLS Clinical Certificates : Loyola University Chicago

    Play Episode Listen Later Feb 28, 2025 28:48


    In this episode Luis is joined by Dr. Kristen Pesavento from Loyola University in Chicago. She was a guest in 2023 when she came to talk about the MS in MLS degree that her institution was offering. This time she joins the podcast to talk about clinical certificates. They are offered on three subjects of the clinical laboratory. These certificates allow you to sit for the categorical certification from the American Society for Clinical Pathology (ASCP) on the areas of Hematology, Blood Banking, and Microbiology. Join in and listen to this episode to get more information on these certificates. Microbiology certification episode: https://rss.com/podcasts/letstalkmicro/1654480/Loyola University MS in MLS episode: https://rss.com/podcasts/letstalkmicro/888449/Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    176: Microbiology kits: Carolina Distance Learning

    Play Episode Listen Later Feb 21, 2025 25:42


    Does your institution teach distance microbiology courses? What do you use to teach students those critical skills required in microbiology? In this episode Luis is joined by Brittany Jackson, a specialist from the Carolina Distance Learning section of the Carolina Biological Supply company. Brittany talks about the kits for microbiology students. Which materials are in those kits? Gram stains? Media? Tune in to learn more.Link to website: https://carolinadistancelearning.com/ready-to-ship/Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    175: The Path to Microbiology: their journey pt. 2

    Play Episode Listen Later Feb 14, 2025 59:51


    A great conversation between three microbiologists! In this episode Luis is joined by Kelli Maddock and Jessica D'Angelo to talk about their careers. Kelli is a previous guest and is currently a section head in microbiology. Jessica is a microbiology supervisor. What inspired them to pursue a career in the laboratory and microbiology? What challenges have they encountered? What is their favorite bug?Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    174: The Path to Microbiology: their journey

    Play Episode Listen Later Feb 7, 2025 75:14


    If you work in laboratory medicine or microbiology, what led or inspired you to choose that career? What have you learned? Do you have a different occupation within the field than the one you started with? This episode is part of a series that features members of the laboratory and microbiology community, who come in and share their journey. They talk about their positions, what they have enjoyed, what challenges they have encountered, and more.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    173: Acid- fast bacilli (AFB) : An Introduction

    Play Episode Listen Later Jan 31, 2025 39:08


    It's time to talk about AFB! What is acid fastness? What are some stains used for acid-fast bacilli? In this episode Luis is joined by Dr. Filipe Cerqueira to talk about AFB. He goes over stains, media, processing of AFB cultures, Runyon groups, and more. He also talks about Nontuberculous mycobacteria (NTM) and provides examples of organisms. Tune in for another great episode . Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    172: Talking AMR: Macrolides and Lincosamides

    Play Episode Listen Later Jan 24, 2025 53:03


    The next episode of the AMR series is here! This time Luis and Andrea are joined by Sonali Kalathiya, a pharmacist and Medical Science Liaison. Sonali joins the podcast to talk about two classes of antibiotics: macrolides and lincosamides. Have you performed a D-test in the lab? What is the purpose of the test? If you have performed it and perform susceptibilities for erythromycin and clindamycin, then you are familiar with these classes of drugs. Tune in to learn more. Also, in the episode Sonali asks Luis about the origin of the Let's Talk Micro logo. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    171: Microbiology and Blood Bank Simulation Lab

    Play Episode Listen Later Jan 17, 2025 38:20


    A simulation laboratory? In this episode Luis is joined by Emily Matthys, an Assistant Professor at Northern Michigan University. Emily teaches at a Medical Laboratory Sciences program and she joins the podcast to talk about a simulation laboratory that was implemented for blood bank and microbiology for medical laboratory technician students. As some labs become more centralized, and end result might be diminished exposure to some areas for medical laboratory science students undergoing their clinical rotations. How does this simulation lab help students? What do the students do in this lab? Tune in for another great episode.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    170: Sulopenem

    Play Episode Listen Later Jan 10, 2025 32:16


    Let's Talk Micro kicks off 2025 with a great new episode. This time Luis is Joined by Dr. Sam Aitken to talk about sulopenem. Have you heard about this oral antibiotic? It was approved by the Food and Drug Administration (FDA) for the use of uncomplicated urinary tract infections (uUTIs). This is an oral penem, and the news of its release has caused different reactions in social media by members of the Microbiology/Infectious Diseases community. What is sulopenem? Why is it combined with probenecid (Orlynvah)? What are the concerns regarding sulopenem? When are carbapenems appropriate for treatment? Tune in for a great episode .Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    169: Health Care Linens: outbreaks and more

    Play Episode Listen Later Dec 20, 2024 35:37


    Working in the microbiology field you might be familiar with outbreaks and health care associated infections (HAI). What about linens in health care? What outbreaks have been associated with health care linens (HCLs)? Are there any regulations regarding their use? What about the MOULD study? Luis is joined by Dr. Alex Sundermann to talk about HCLs, regulations, mold-associated outbreaks, and more. Link to Dr. Sundermann's article:https://www.infectioncontroltoday.com/view/health-care-linens-underrecognized-risk-infection-prevention-controlQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    168: Pasteurella: species, media, and more

    Play Episode Listen Later Dec 13, 2024 30:01


    Following the episode with Dr. Kelli Maddock, Luis decided to talk about his favorite organism: Pasteurella! This time Luis goes into more detail. What are some species of Pasteurella? Which animals are they found on? Which species are isolated from humans? This episode also covers media, biochemicals, diseases, and more.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    167: Talking AMR: Oxazolidinones

    Play Episode Listen Later Dec 6, 2024 42:21


    Luis and Andrea are back to talk antibiotics! This time they are joined by David Allen, an infectious diseases (ID) pharmacist to talk about another class : oxazolidinones. How familiar are you with this class? Have you performed testing on linezolid in the lab? Then you might be somewhat familiar. What is the mechanism of action? Intrinsic resistance? What organisms does this class have activity against? Tune in to find out.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    166: Pasteurella multocida: capsular groups and more

    Play Episode Listen Later Nov 29, 2024 31:54


    A Pasteurella multocida episode! In this episode Luis is joined by Dr. Kelli Maddock to talk about P. multocida. Kelli was a previous guest and this time returns to talk about the capsular types of P. multocida. What are these types? What diseases are associated with them? What about hemorrhagic septicemia? Kelli also talks about a model developed to look for hemorrhagic septicemia types of P. multocida using MALDI-TOF MS. Don't miss out on a great episode!Link to Dr. Maddock's article: https://www.sciencedirect.com/science/article/pii/S0167701224001799?via%3DihubQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    groups ko venmo maddock pasteurella maldi tof ms
    165: E. coli: NDM and PBP variants

    Play Episode Listen Later Nov 22, 2024 34:42


    As a new microbiologist sometimes terms like beta-lactams, beta-lactamases, or penicillin-binding proteins (PBPs) can be confusing. Are you familiar with the New-Delhi metallo-beta-lactamase (NDM) and its variants? How many are there? How does this enzyme affect beta-lactam drugs?How about NDM-5? In this episode Luis is joined by Dr. Raneem Pallotta, an infectious diseases pharmacist. In the episode we go over these terms. Dr. Pallotta also talks about an NDM-producing E. coli that harbors PBP3 variants. How do these variants affect beta-lactam antibiotics? Tune in for a great episode.Link to Dr. Pallotta's article: https://www.contagionlive.com/view/ndm-producing-e-coli-harboring-pbp-insert-variants-emerges-as-new-threatLink to other articles: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-017-1012-8https://pmc.ncbi.nlm.nih.gov/articles/PMC5527577/https://pubmed.ncbi.nlm.nih.gov/34800710/https://pubmed.ncbi.nlm.nih.gov/32040179/https://pubmed.ncbi.nlm.nih.gov/25634992/https://www.idsociety.org/practice-guideline/amr-guidance/Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    164: MicroClinUy (En Español/ In Spanish)

    Play Episode Listen Later Nov 20, 2024 62:47


    En este episodio en español, Luis conversa con la Dra. Verónica Seija. La Dra. Seija es especialista en microbiología y tiene un blog, redes sociales y un podcast donde habla sobre microbiología bajo el nombre MicroClinUY. ¿ Qué motivó a la Dra. a comenzar a hablar sobre microbiología? ¿Qué tipo de contenido encontramos en sus redes sociales y el podcast? ¿Dónde encuentran el podcast? Sintonizen y no se pierdan de una gran conversación. Enlace al podcast: https://www.microclin.uy/podcastEnlace a la página web de MicroClinUy: https://www.microclin.uyPreguntas? Comentarios? Envíen un correo electrónico a letstalkmicro@outlook.comDesean apoyar al podcast? Pueden hacerlo de la siguiente forma:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    163: Talking AMR- Glycopeptides

    Play Episode Listen Later Nov 15, 2024 56:58


    Luis and Andrea return with a new episode of the AMR series. This time, they are joined by Dr. Nikunj Vyas, and they go over glycopeptides. Whether you are a microbiologist, provider or an ID pharmacist, you are familiar with vancomycin. What is the mechanism of action of glycopeptides? Is there intrinsic resistance? How does a difference in minimum inhibitory concentration (MIC) affect treatment? Tune in to learn more. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: Veterinary Microbiology

    Play Episode Listen Later Nov 8, 2024 38:05


    Throwback to a great episode! Have you worked in a veterinary laboratory? What kind of training do you need? Are you able to work in one as an MLS? Have you thought about what a culture from a veterinary specimen might look like? How do you work those up? What about antimicrobials and guidelines? Kelli Maddock and Sarah Gefroh from the North Dakota State University Veterinary Diagnostic Laboratory join the podcast to answer these questions. They are Medical Laboratory Scientists with experience in human medicine who switched to veterinary medicine.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: Biochemicals- catalase and coagulase

    Play Episode Listen Later Oct 24, 2024 24:07


    One of the first episodes published and one of the most downloaded episodes. In it Luis goes over catalase and coagulase. What are biochemicals? How are they useful in the identification of bacteria? In this episode I start going over biochemicals: catalase and coagulase, two of the most commonly used biochemicals in Clinical Microbiology.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: Media

    Play Episode Listen Later Oct 17, 2024 22:10


    The first episode! Share it with students and peers, In it Luis talks about agar. What is agar? What do we use it for? Various types of agar are discussed like blood agar and MacConkey along with their uses.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    159: A novel microbicide for Neisseria gonorrhoeae

    Play Episode Listen Later Oct 10, 2024 25:26


    A microbicide for Neisseria gonorrhoeae? In this episode Luis is joined by Dr. María Rendón from the University of Arizona. Dr. Rendón joins the podcast to talk about a novel microbicide that was developed which has activity against N. gonorrhoeae . How does it work? Does it affect commensal Neisseria? What about methylation signatures? Tune in to learn more about this microbicide.Link to articles referenced in episode:http://microbialcell.com/researcharticles/2019a-so-microbial-cell/https://www.cell.com/action/showPdf?pii=S1931-3128%2819%2930347-6Link to study: https://journals.asm.org/doi/abs/10.1128/aac.00794-24?doi=10.1128/aac.00794-24Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    158: Joint Infections PCR Panel

    Play Episode Listen Later Oct 3, 2024 24:22


    Are you familiar with the BioFire system? If you work in microbiology you probably are. How about a joint infections panel for this system? In this episode Dr. Angelica Moran and Jekzaly Arellano join the podcast to talk about a study they did evaluating this panel. Why are joint infections difficult to diagnose? Tune in to learn more.Link to article: https://journals.asm.org/doi/10.1128/jcm.00182-24Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    157: Lactobacillus

    Play Episode Listen Later Sep 26, 2024 24:23


    This week Luis continues going over gram-positive rods: this time Lactobacillus. What is the morphology of Lactobacillus? Biochemicals? Which species are typically seen in humans? Is it pathogenic or normal flora? What about susceptibilities? All great questions. Tune in to find out about this genera. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    156: Actinotignum schaalii

    Play Episode Listen Later Sep 19, 2024 26:27


    If you work with MALDI-TOF MS and you read urine cultures, you probably have seen this organism : Actinotignum schaalii. Is this organism significant? What is the gram stain? Biochemicals? Other questions come up such as we do report it? Do we perform susceptibilities ? Tune in to learn about this emerging pathogen and get answers to all these questions.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    ko venmo maldi tof ms
    155: ASCP: Microbiology Certifications

    Play Episode Listen Later Sep 12, 2024 42:18


    Those of you that work in the laboratory as Medical Laboratory Scientists know that you need to be certified. How can you get certified in microbiology? Even if you haven't completed a Medical Laboratory Sciences program, is there a way to get certified? In this episode Dr. Amanda Harrington joins Luis to answer questions regarding certification in Microbiology from the American Society for Clinical Pathology (ASCP), including information about the Specialist in Microbiology Certification.Link to certifications in Microbiology from ASCP: https://www.ascp.org/content/board-of-certification/get-credentialed?srsltid=AfmBOoo4i6_pt4DLUEKUJmclab7AJkFp1bZlPGibNnIqLb-jgp3JGgvO# Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    154: Dengue

    Play Episode Listen Later Sep 5, 2024 58:46


    Dr. Rodney Rohde returns to the podcast! This time he joins Luis to talk about dengue. You might hear reports about increases in cases in some areas, so it was a good time to talk about it. What is dengue? What type of virus it is? Vectors? Prevention? Is there a vaccine? Tune in to learn more about this virus.Link to Dr. Rohde's dengue article: https://asm.org/articles/2024/august/dengue-is-spreading-is-warmer-weather-to-blameMore information about dengue: https://www.cdc.gov/dengue/index.htmlLink to Dr. Rohde's Podcast: https://www.contagionlive.com/view/from-pathogen-to-infectious-disease-diagnosis-taking-on-potential-standard-of-care-diagnostic-opportunitiesQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    153: Bone and Joint Infections

    Play Episode Listen Later Aug 29, 2024 38:16


    If you work in a clinical microbiology laboratory, you might have worked with bone and joint samples. In this episode we dive deeper into bone and joint infections. Luis is joined by Dr. Joan Gomez-Junyent, an Infectious Diseases physician at Hospital Del Mar in Barcelona, Spain. Dr. Gomez goes over different types of infections. What organisms are typically seen? Is there a typical treatment of choice? How about antimicrobial resistance? Tune in to hear Dr. Gomez go over these questions and more. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    152: Potential antibiotics: lolamicin

    Play Episode Listen Later Aug 22, 2024 27:26


    Season 4 is here! Tune in for the first episode. Luis talks to Dr. Paul Hergenrother about lolamicin. Dr. Hergenrother is a Professor of Chemistry at the University of Illinois in Urnaba-Champaign. He was previously a guest in the podcast (fabimycin episode) and this time he returns to talk about another potential antibiotic candidate: lolamicin. What is lolamicin? What is the mechanism of action? What organisms does it have activity against? Does it affect the gut microbiota? Link to Dr. Hergenrother's article: https://www.nature.com/articles/s41586-024-07502-0Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: Streptococcus anginosus group

    Play Episode Listen Later Aug 15, 2024 21:00


    Originally published in May 26, 2022, in this episode Luis continues going over beta hemolytic strep: this time Streptococcus anginosus group. What are the species that comprise this group? What is the Lancefield group? What about biochemicals and morphology? Have you heard of Streptococcus pseudoporcinus? Tune in to find out.Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: LPC-233: An LpxC inhibitor

    Play Episode Listen Later Aug 1, 2024 18:24


    Originally published in November 30 2023, in this episode, Dr. Pei Zhou from the Duke University School of Medicine joins the podcast and talks to Luis about LPC-233. This is a compound that shows bactericidal activity against some gram-negative rods. LPC-233 is an LpxC inhibitor. What is LpxC? How does LPC-233 works? What are some challenges of developing LpxC inhibitors? Tune in to find out.Link to article: https://doi.org/10.1126/scitranslmed.adf5668Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: Fabimycin: an antibiotic candidate

    Play Episode Listen Later Jul 25, 2024 21:27


    Originally published on October 13, 2022, in this episode Luis talks to Dr. Paul Hergenrother from the University of Illinois. Dr. Hergenrother talks about Fabimycin, an antibiotic candidate for gram negative bacteria. What is Fabimycin's mechanism of action? To which bacteria does it show activity against? Any intrinsic resistance? Tune in to find out.Link to article: https://pubs.acs.org/doi/10.1021/acscentsci.2c00598Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    151: Ace the Boards: Microbiology

    Play Episode Listen Later Jul 18, 2024 33:27


    A great way to end season 3! In this episode Dr. Cullen Lilley talks to Luis about his textbook : A Concise Review of Clinical Microbiology . Dr. Lilley is a pathology resident, with a Microbiology background, and a Let's Talk Micro listener! Tune in to learn about what is in this microbiology textbook, what is Ace my Path, and more. Link to Ace My Path: https://www.acemypath.comLink to textbook: https://a.co/d/gGDiZ8ZQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    150: Sterile body fluids and Multiplex PCR

    Play Episode Listen Later Jul 11, 2024 38:24


    In the laboratory we routinely use molecular testing to identify organisms in blood cultures. We also incubate body fluids in blood culture bottles. What if we could use those same tests to identify organisms from body fluids? In this episode Christina Cox and Joel Mortensen join the podcast to talk about this. They performed a study where they used the cobas® eplex system (GenMark) to identify organisms recovered from sterile body fluid cultures. Tune in to hear about the results and the applications of this in the clinical laboratory.Link to article: https://doi.org/10.1128/jcm.01611-23Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    TBT: Respiratory cultures

    Play Episode Listen Later Jul 4, 2024 41:39


    Throwback Thurdsday! This week shows an episode originally released in 2022 where Luis talks to Andrea Prinzi about respiratory cultures. What are the types of respiratory cultures? What are the pathogens? What is the significance of yeast in respiratory cultures? Sputum rejection? Tune in to this episode to learn more about these cultures.Link to article about sputum rejection that Dr. Prinzi mentions in the episode: https://journals.asm.org/doi/pdf/10.1128/jcm.16.4.627-631.1982Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    149: Talking Microbiome

    Play Episode Listen Later Jun 27, 2024 38:20


    In this episode Luis is joined by Dr. Crystal Icenhour to talk about the microbiome, more specifically, the human microbiome. What organisms typically make up the microbiome? Do they change between areas of the body? What are some applications for the microbiome? Tune in for another great episode. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    148: Bonus: Live from ASM Microbe 2024

    Play Episode Listen Later Jun 25, 2024 29:44


    ASM Microbe 2024 took place in Atlanta Georgia from June 13-17. Check out this bonus episode where Luis discusses his two events, favorite posters, and more. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    147: Research in Medical Laboratory Science: moose, bears, ESBL, and more

    Play Episode Listen Later Jun 20, 2024 75:56


    Research in MLS? In this episode Grace Leu-Burke, director of the Medical Laboratory Sciences (MLS) program at the University of Alaska in Anchorage, returns to the podcast. She talks to Luis about the research program the MLS students are involved in. They culture scat from moose, bears, and geese, perform identification and susceptibility testing, and more. What kind of antimicrobial resistance is found in this sample population? Tune in to learn more about this research. Link to JAM meeting registration: https://www.labjam.org/event/cc75ef76-49db-4175-8440-d194382bcf05/websitePage:d132f4da-b01b-4c7c-9ea8-5866e2b0f8caTitle of Grace's Talk: “Emerging Antimicrobial Resistance, A One Health Model”Thursday July 11th at 9:30amQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    146: Urinary Tract Infections (UTIs)

    Play Episode Listen Later Jun 13, 2024 25:05


    In this episode Luis talks to Dr. Madeline Barron about urinary tract infections (UTIs). Clinical Microbiologists work closely with urine cultures and recover many organisms and perform susceptibility testing. However some questions come to mind. What causes a UTI ? What organisms are typically seen in UTIs? Any treatment options other than antimicrobials? Tune in to find out.Madeline Barron's article: https://asm.org/articles/2024/may/why-urinary-tract-infections-uti-recurQuestions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    145: Talking AMR: Polymyxins (lipopeptides)

    Play Episode Listen Later Jun 6, 2024 41:42


    Luis and Andrea are back to talk AMR! This time they are joined by Dr. Alyssa Walker to go over polymyxins . As usual they go over important questions such as mechanism of action, intrinsic resistance, applying the interpretation of one drug to another, and more. What media used in the laboratory has colistin in it? Don't miss another great episode. Questions? Feedback? Send those to letstalkmicro@outlook.comWant to support the podcast? Here's how:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

    144: Serie en Español: Tinción de Gram

    Play Episode Listen Later Jun 4, 2024 42:42


    Regresa la serie en Español. Luis y Maridaliz discuten la tinción de gram. Cómo se prepara la laminilla? Cúales son los reactivos y el proceso? Qué diferencia hay entre bacterias gran-positiva y gram-negativa? Sintonizen a este episodio para aprender sobre una de las pruebas mas importantes en Microbiología Clínica. Preguntas? Comentarios? Envíen un correo electrónico a letstalkmicro@outlook.comDesean apoyar al podcast? Pueden hacerlo de la siguiente forma:Venmo: https://venmo.com/u/letstalkmicroBuy me a Ko-fi: https://ko-fi.com/letstalkmicro

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