Podcasts about sea phages

In this episode, Celina Tanbari from the University of Ottawa interviews Dr. Adam Rudner about the fascinating world of bacteriophages—viruses that target and kill bacteria. Dr. Rudner is the coordinator of the University of Ottawa's Phage Hunters program, a member of the SEA-PHAGES and SEA-GENES programs, and an Associate Professor in the Department of Biochemistry, Microbiology, and Immunology. Dr. Rudner shares with us his expert opinion on a study that isolated bacteriophages from human skin to kill infection-causing bacteria. The discussion delves into the therapeutic potential of phages, their involvement in biofilms and lysogeny, and the challenges associated with utilizing phages in medicine. This episode was produced and written by Emma Etchells Foisy, hosted by Celina Tanbari, and audio edited by Sam Rogers. Copyrights are reserved to BEaTS Research Radio and the University of Ottawa.If you are interested in learning more about the paper we discussed today, you can find it through the following link: https://pmc.ncbi.nlm.nih.gov/articles/PMC10117716/Learn more: https://www.uottawa.ca/faculty-medicine/dr-adam-rudner 0:03 | BEaTS and host introduction.0:38 | Introducing Dr. Rudner.1:05 | What are bacteriophages?1:41 | Phages as a possible solution to antibiotic-resistant bacteria 2:41 | What are biofilms, and how do they affect bacterial infections?5:01 | Phage BE01 and how lysogeny can affect phage therapy.8:52 | Phage BE04 and the potential of phages in replacing antibiotics. 10:55 | Squashing the stigma, phages only attack bacteria.12:35 | Potential challenges with phage therapy. 14:08 | Dr. Rudner's take-home message. 14:36 | Thank yous and credits!Soundtrack, Chillout by AudioCoffee | https://www.audiocoffee.net/Music promoted by https://www.chosic.com/free-music/all/Creative Commons CC BY-SA 3.0https://creativecommons.org/licenses/by-sa/3.0/

Matters Microbial #62: Should I Stay, or Should I Go—How Bacteriophage are Released from Host Cells October 23, 2024 Today, Dr. Jolene Ramsey, of the Biology Department of Texas A&M University and Affiliate of the Center for Phage Technology, joins the #QualityQuorum to discuss how bacteriophages release themselves from host cells, her efforts to teach students to work with the primary literature, and her own path to the microbial sciences.   Host: Mark O. Martin Guest: Jolene Ramsey Subscribe: Apple Podcasts, Spotify Become a patron of Matters Microbial! Links for this episode A truly wonderful reminder video about bacteriophages. A link with a 3D printer design of capsid model pieces (personally, I am really interested in making one of these!). A video demonstrating how the capsid model pieces self assemble—something like real viruses can? An essential book about bacteriophage authored by my late friend Merry Youle. A fine book describing how bacteriophages can be used to fight bacterial diseases. This “first person” book by #MattersMicrobial podcast guest Steffanie Strathdee about how she was able to use bacteriophages to save her husband's life is a must read. A link to the Citizen's Phage Library. A link to the truly fabulous (yes, I am jealous I am not part of this program) CURE program SEA-PHAGES and SEA-GENES for undergraduate students. A remarkable illustration of T4 bacteriophage bursting from host cells by the scientist-artist David Goodsell. The organization that designed Dr. Ramsey's laboratory logo. A link to the Clinical Genome Curation for Human Genes.   A link to the CACAO website for biocuration competition. A recent Ramsey lab mini-review on phage classification. A link to the Center for Phage Technology. The Ramsey lab Instagram page. The Ramsey lab YouTube channel. Dr. Ramsey's laboratory website. Dr. Ramsey's faculty website. Intro music is by Reber Clark Send your questions and comments to mattersmicrobial@gmail.com

Hannah Deyell from the University of Ottawa interviews Dr. Adam Rudner on his current research and experience. Dr. Adam Rudner is an Associate Professor at the University of Ottawa in the Department of  Biochemistry, Microbiology and Immunology, a member of the SEA-PHAGES and SEA-GENES programs, and the coordiantor of uOttawa Phage Hunters. The primary research focuses of Dr. Rudner's team are exploring the potential applications for baceteriophage therapy, building essential research skills, and exploring bacteriophages with interesting genomic sequences. In this episode, Dr. Rudner explores how bacteriophages can target antibiotic resistance for patients with disseminated bacterial infections, how his lab is working to increase the pool of available phages, and how personalized medicine can be used to improve patient outcomes with phage therapy. Learn more: https://rudnerlab.med.uottawa.ca/Adam_Rudner_Lab/Home.html00:00  | Introduction01:00  |  Dr. Adam Rudner's recent research focuses and ongoing projects 02:13  |  The roles of bacteriophages and how they function to treat bacterial infections03:04  |  Advantages of bacteriophage therapy over tradtional antibiotics in the context of antibiotic resistance 04:13  |  Challenges associated with research related to bacteriophages / limitations of bacteriophage therapy 05:44  |  Primary motivators for using engineered bacteriophages as a treatment for 15-year old cystic fibrosis patient07:51  |  The purpose of engineering bacteriophages to effectively treat the patient10:23  |  The undergraduate SEA-PHAGES Program 11:48  |  Dr. Adam Rudner's research advice to undergraduate students 13:17  |  ConclusionCredits for music: Soundtracks by the Underground Drive. All rights reserved. Listen morehttps://music.apple.com/ca/artist/the-underground-drive/1571062779https://open.spotify.com/artist/4sCJG8TMQyTZ9FDd1JjJmR

Life: our planet is teeming with it. The staggering diversity of the biological world is visible everywhere, from tiny insects to sprawling trees, from orchids and corgis to mold and lemurs and banana slugs and oyster mushrooms and . . . you get the idea. But even the boundless wonder of the macroscopic world may pale in comparison to all the life we cannot see. Jocelyn and Bradley are joined this week by Dr. Hannah Gavin, a veritable Willy Wonka of microbes, who introduces us to a vast, complex world of dazzling drama invisible to the naked eye but present all around us—and even within us. Hannah shares how being “professionally curious” led her to research questions at the intersection of microbiology, ecology, and human health. She discusses the far-reaching potential of the gut microbiome to impact our physiology; what differentiates “beneficial” bacteria from pathogens; and how microbes can perform valuable environmental services like digesting plastic. Along the way, she explains the differences between viruses and bacteria and bacteriophages (oh my!), and how viruses challenge our understanding of life itself. The friends also discuss how the COVID-19 pandemic has opened new spaces for dialogue between scientists and non-scientists, creating opportunities for us to learn together about the sometimes terrifying, sometimes beautiful, always awe-inspiring power of the microbiological world.Follow Hannah on Twitter @micro_cultured, and learn more about her amazing work at the links below!Harvard’s Microbial Sciences Initiative: https://msi.harvard.edu/SEA-PHAGES program: https://seaphages.org/“World of Viruses” exhibit (Harvard Museums of Science and Culture): https://hmsc.harvard.edu/world-viruses“A Fascinating World of Viruses” (Hannah on the HMSC Connects! podcast):https://hmscconnects.podbean.com/e/a-fascinating-world-of-viruses-with-microbiologist-hannah-gavin/“The Deadliest Being on Planet Earth – The Bacteriophage” (Kurzgesagt): https://youtu.be/YI3tsmFsrOgRelated episodes:Turkey, Stuffing, and Other Gastronomic Experiments (Soon Kiat Lau): https://podcasts.apple.com/us/podcast/24-soon-kiat-lau-turkey-stuffing-other-gastronomic/id1471423633?i=1000458033346 Our Soils, Ourselves (Yamina Pressler): https://podcasts.apple.com/us/podcast/39-yamina-pressler-our-soils-ourselves/id1471423633?i=1000469510259Everyone Has Herpes (Lisa Poppe): https://podcasts.apple.com/us/podcast/8-lisa-poppe-everyone-has-herpes/id1471423633?i=1000446370166

In this episode of Cell Cultured, Sid and now co-host Neeshil discuss the use of antibodies to eradicate disease spread by mosquitoes and organ systems modeled on microchips. They also talk about how sea phages, collected by students all over the country including at Pitt, were used to treat a nasty bacterial infection.

Bacteriophages (“phages”), or bacterial viruses, are the most abundant biological entity on the planet, and the microbial world is shaped by these predators and parasites. The ability of bacteriophages to specifically target and kill their prey is being explored as an alternate therapy to antibiotics against various bacterial diseases. Dr. Graham Hatfull is a professor at the University of Pittsburgh who studies Mycobacterium tuberculosis, the causative agent of tuberculosis, and the phages that infect it. Hatfull directs the Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program along with the Howard Hughes Medical Institute. Hatfull talks about how the SEA-PHAGES program has allowed entering students at more than 100 colleges and universities around the country to discover thousands of new phages, how phages isolated from the program were used to save the life of a patient infected with Mycobacterium, what the prospects are for phage therapy being used as treatment for other diseases, how bacterial resistance to phage infection impacts phage therapy, and how important research experience can be for students. microTalk was joined by Dr. Jimmy Ballard when this podcast was recorded at the ASM Microbe 2019 conference in San Francisco, CA. The microCase for listeners to solve is about Buck Shott, an aging Western movie stunt double who comes down with a potentially fatal infection after filming an action scene for “The Old, the Ancient, and the Geriatric”. Participants: Karl Klose, Ph.D. (UTSA) Graham Hatfull, Ph.D. (University of Pittsburgh) Jimmy Ballard, Ph.D. (University of Oklahoma) Janakiram Seshu, Ph.D. (UTSA) Mylea Echazarreta (UTSA)

The TWiV team notes the passing of Tom Steitz, an outbreak of acute flaccid myelitis in the US, a continuing Ebola virus outbreak in DRC, respiratory vaccinia due to inhalation of ground up rabbit skin, and how a human papillomavirus capsid protein directs virus-containing endosomes towards the nucleus. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Sea Phages program and application materials Tom Steitz, 78 (NY Times) AFM outbreak, US (CIDRAP) AFM surveillance (CDC) Acute Flaccid Paralysis and Enteroviral Infections (Curr Inf Dis Rep) EV-D68 neurotropism (bioRxiv) Ebola virus outbreakcontinues in DRC (CIDRAP) Vaccinia virus respiratory infection (Int J Inf Dis) HPV L2 penetrates endosome, brings virus retro (Cell) Letters read on TWiV 515 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - Germ City-Microbes and the Metropolis Museum Exhibition Alan- Nikon 2018 Small World in Motion competition Kathy- Ion Torrent Sequencing (video) Dickson- 2018 Nobel Prizes Economics Kathy- Ocean-centric map Vincent - Intelligence Matters Podcast: Anthony Fauci on threats from epidemics Listener Picks Gonsalo - Cells at Work (Manga, Anime) Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiVumvirate reviews this years crop of Nobel Prizes, and how cells prevent leakage of mitochondrial double-stranded RNA into the cytoplasm, which would otherwise lead to the production of interferon. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Sea Phages program and application materials Plant biologists penalized by CNRS (The Scientist) 2018 Nobel Prize in Medicine (pdf) 2018 Nobel Prize in Chemistry (pdf) 2018 Nobel Prize in Physics (pdf) 2018 Nobel Peace Prize (Nobel) Mitochondrial dsRNA triggers IFN (Nature) Letters read on TWiV 514 Timestamps by Jolene. Thanks! Weekly Science Picks 1:28:33 Alan- Compound Interest chemistry graphics Kathy- Writing letters of recommendation Guidelines Trix & Psenka Schmader et al. Madera et al. Dickson- Japan Fireworks Vincent - The Game of T-Cells and Apple Park Lego Listener Picks Steve - Shomu's Biology Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Bill Jacobs talks about developing mycobacterial genetic tools and using them to discover ways to shorten TB treatment. He also talks about the SEA-PHAGES program that allows high-school students to participate in phage discovery. Host: Julie Wolf Subscribe (free) on iPhone, Android, RSS, or by email. You can also listen on your mobile device with the ASM Podcast app. Julie's biggest takeaways: The challenges of working with an easily aerosolized bacterium are aided by complementary studies on a noninfectious relative. M. smegmatus doesn’t colonize mammals and grows slower, giving researchers the opportunity to acclimate themselves to working with mycobacterial cultures. Jacobs was the first scientist to introduce DNA into M. tuberculosis using a phasmid - part plasmid, part mycobacterial phage. The first phage came from Jacobs’ dirt yard in the Bronx, so he named it BxB1 for the Bronx Bomber. Another phage, TM4, became the workhorse phasmid when Jacobs cloned an E. coli cosmid sequence into a nonessential part of the phage genome. It replicates in E. coli as a plasmid but becomes a phage inside Mycobacteria, facilitating manipulation. The shuttle phasmids allowed transposon delivery to make transposon libraries, and the creation of gene knockouts. To this day, we use Ziehl-Neelsen staining to differentiate acid-fast mycobacteria from gram-positive or gram-negative bacteria - the mycolic acids on the outer part of the envelope make up some of the longest microbial lipid chains. But mycobacteria can regulate its acid-fast positive or negative status; the acid-fast negative organisms are a persistent population that are often ignored inside of patients. 99.99% of M. tuberculosis bacteria are not persistent, but the last 0.1% have entered into a persistent state expressing many stress proteins that help them become refractory to killing. A normal course of antibiotic chemotherapy for patients is six months. If infected with a strain resistant to the two frontline drugs, that time goes up to two years. The problem is even greater in extremely multidrug resistant (XDR) strains. What we really need is a way to understand persistence and a way to shorten chemotherapy. That’s why were were absolutely amazed when we discovered that cysteine with isoniazid completely sterilizes Mtb cultures in vitro and in vivo! The culture is sterilized because the bacteria can’t form persisters. Vitamin C co-treatment with antibiotics may lead to a shortened course of therapy for TB treatment. Neutralizing antibodies to the herpesvirus glycoprotein have been the dogma for protecting from herpes. Jacobs and his colleagues discovered that a vaccine based on a glycoprotein-knockout virus confers sterilizing immunity not through neutralizing antibodies but through antibody-dependent cell cytoxicity (ADCC). This ADCC response may also be important to develop a more effective TB vaccine.   Featured Quotes (in order of appearance): “You’ll never know how bad your aseptic technique is until you start working with tuberculosis!” “I think part of the reason I had the opportunity to develop genetics for TB - it’s not like it wasn’t important to do - but a lot of people were disappointed when working with the organism.” “We’re about to take TB genetics to where yeast genetics is.” “One of the tubicle bacilli’s greatest powers or one of its most important phenotypes is that it has the ability to persist, which means it has the ability to tolerate killing effectors, either killing by the immune system or killing by bactericidal drugs.” “I took students to the Bronx Zoo, and over by the zebra pen, I sniffed and said ‘I smell a phage!’ In fact, that’s not crazy - anyone who plants flowers knows what good soil smells like, and in the good soil, you’re smelling the bacteria that live in the soil, the Streptomyces and Mycobacteria. I reached down and grabbed that dirt, and when we went back to work we isolated BxE1.” “I’ve never met a phage I wasn’t excited about!” “I now believe that most pathogens do not ‘want’ ADCC antibodies to be made, and they have immune evasion strategies where they skew the immune response to get the wrong antibodies. Since the time we published our first paper, numerous groups have shown that correlates of protection for HIV, for influenza, and for Zika, turn out to be ADCC antibodies.” “Genetics is the mathematics of biology!”   Links for this episode Bill Jacobs lab site NYTimes story on 1993 rapid diagnostic test using luciferase AACJournal: Vitamin C potentiates the killing of Mycobacterium tuberculosis by the first-line tuberculosis drugs isoniazid and rifampicin in mice Cell: Origins of highly mosaic mycobacteriophage genomes SEA-PHAGES program eLife: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity mBio: Dual-reported mycobacteriophages (Φ2DRMs) reveal preexisting Mycobacterium tuberculosis persistent cells in human sputum Tuberculosis - Its cause, cure and prevention [1914] (pdf)   Send your stories about our guests and/or your comments to jwolf@asmusa.org.  

Ann Findley, Chris Gissendanner, and Allison Wiedemeier from the University of Louisiana Monroe join for discussion of their SEA-PHAGES program and cluster prediction using the Phage Enzyme Tool (PET) (available at: http://tinyurl.com/pudnr79).

Ann Findley, Chris Gissendanner, and Allison Wiedemeier from the University of Louisiana Monroe join for discussion of their SEA-PHAGES program and cluster prediction using the Phage Enzyme Tool (PET) (available at: http://tinyurl.com/pudnr79).

Recent improvements to Phamerator are highlighted and the new SEA-PHAGES app is revealed!. Signals: http://tinyurl.com/mr52vwj PMID of papers discussed: 23945046.

Recent improvements to Phamerator are highlighted and the new SEA-PHAGES app is revealed!. Signals: http://tinyurl.com/mr52vwj PMID of papers discussed: 23945046.