Podcasts about sdhi

As biological technologies continue to advance, many growers are exploring how best to integrate them into their farming operations. Nevada Smith, Head of Marketing North America, and Robert Blundell, Research Plant Pathologist, both with Pro Farm Group, highlight the role of biological pesticides and biofertilizers in sustainable winegrowing. Biological pesticides, derived from microbial sources or natural products such as plants, fungi, bacteria, or nematodes, play a crucial role in pest management by inhibiting or delaying growth or directly causing pest mortality. Understanding which biological products to use and when to apply them within an integrated pest management system is essential for maximizing their effectiveness. Biofertilizers, which enhance plant health and resilience to abiotic stresses, are another key tool for sustainable viticulture. Nevada and Robert discuss the growing importance of these technologies in improving soil health and supporting long-term agricultural productivity. Resources:         REGISTER: 5/9/25 Biochar Field Day 117: Grapevine Mildew Control with UV Light 123: What is Happening in Biologicals for Pest Management and Plant Health 266: Soft Pesticide Trial: Powdery Mildew, Downy Mildew, Botrytis, and Sour Rot Healthy Soils Playlist Integrated Pest Management (IPM) Principles ProFarm What are Biopesticides? Vineyard Team Programs: Juan Nevarez Memorial Scholarship - Donate SIP Certified – Show your care for the people and planet   Sustainable Ag Expo – The premiere winegrowing event of the year Vineyard Team – Become a Member Get More Subscribe wherever you listen so you never miss an episode on the latest science and research with the Sustainable Winegrowing Podcast. Since 1994, Vineyard Team has been your resource for workshops and field demonstrations, research, and events dedicated to the stewardship of our natural resources. Learn more at www.vineyardteam.org.   Transcript [00:00:00] Beth Vukmanic: As biological technologies continue to advance, many growers are exploring how to best integrate them into their farming operations. [00:00:13] Welcome to Sustainable Wine, growing with Vineyard Team, where we bring you the latest in science and research for the wine industry. I'm Beth Vukmanic, executive director. [00:00:23] In today's podcast, Craig McMillan, critical resource Manager at Niner Wine Estates. With Longtime SIP certified Vineyard in the first ever SIP certified winery speaks with Nevada Smith Head of Marketing North America and Robert Blundell research plant pathologist, both with Pro Farm Group. Together, they highlight the role of biological pesticides and bio fertilizers in sustainable wine. Growing [00:00:49] biological pesticides are derived from microbial sources or natural products such as plants, fungi, bacteria, or nematodes. They play a crucial role in pest management by inhibiting or delaying growth or directly causing pest mortality [00:01:04] Understanding which biological products to use and when to apply them within an integrated pest management system is essential for maximizing their effectiveness. [00:01:13] Bio fertilizers, which enhance plant health and resilience to abiotic stresses are another key tool for sustainable viticulture, Nevada and Robert discussed the growing importance of these technologies and improving soil health and supporting long-term agricultural productivity. [00:01:30] If you're gonna be in Paso Robles, California on May 9th, 2025. Join us at Niner Wine Estates for a Biochar Field day. This interactive morning features live demonstrations and expert discussions on the benefits of biochar for soil health and sustainable farming. Learn how to integrate biochar into your farming operations through practical insights and hands-on experiences. Go to vineyard team.org/events or look for the link in the show notes to get registered. [00:02:00] Now let's listen in.   [00:02:05] Craig Macmillan: My guest today are Nevada Smith. He is Head of Marketing North America and Robert Blundell, who's a research plant pathologist, both with Pro Farm Group. Thank you for being on the podcast [00:02:15] Rob Blundell: Thank you, Craig. [00:02:16] Nevada Smith: Thank you. [00:02:18] Craig Macmillan: Today we're gonna be talking about bio pesticides and we might as well start with the the basics. What is a biological pesticide? Robert, why don't you start? [00:02:26] Rob Blundell: Yeah, that's a good question, Craig. And and you know, honestly, it's. So when I first was kind of thinking about this, it's not as simple explanation as you might think. It's a constantly kind of evolving term and depending on who you are asking, you can get a, a very different answer. And it's, it's really kind of this large umbrella term. [00:02:42] . It's kind of a microbially based product or natural product typically derived from a plant, fungi, bacteria, nematode, you know. That pretty much has the ability to inhibit or delay the growth or, you know, cause the death of a pest. [00:02:56] And you know, with the term biological pesticide, pesticide being extremely broad whether it's, you know, insect, fungi, even rodent, you know, rodent sides, things like that. So yeah, again, it's a very broad term and different, different grooves, different commodities are gonna kind of have their own explanation. [00:03:09] Even the EU has a different, I think definition versus the EPA as well. So it's an evolving, evolving term. [00:03:15] Craig Macmillan: What about you, Nevada? Do you have anything to add to that? [00:03:17] Nevada Smith: I'm kind of with Robert, it's almost like sustainability. What does that mean? It means to me, I get to keep farming every year. But I think for everyone else it might have different definitions. And I think basically the, the premise is, is it's biologically based. It's based on a living organism, something that we can repeat, regrow, and, you know, the societal part of it, bio pesticide, it means it's acting or killing or helping mitigate pest. For proform have a biologically based strategy. And so we, that's what we deliver is those type of tools. [00:03:50] Craig Macmillan: One of the major pets on grapes is powdery mildew. Around the globe. Probably the major pest overall, I would say fungal disease. I have been seeing a lot of increase in the use of bio pesticides specifically for powdery mildew, some in organic systems, some in more traditional sustainability oriented systems. [00:04:09] What kind of mechanisms are there out there in the biological world for managing powdery mildew and how does that, how do they work? Nevada, do you wanna start? [00:04:18] Nevada Smith: Yeah, so for biological pesticides, there's sort of different categories and I'll even. Even throwing some sort of organic pesticides as well into this whole mix. I think as a grower or a wine processor, you have a choice and it's like, either I'm going conventional, I'm looking to maximize my value proposition on my vineyard or my process my wines. And so one of the ways we really think about this is how do you integrate bio pesticides into the overall spray for bio mildew, like our winemaker at our place they always say, Hey, if it's more than 3% power mildew it's a no go. It's a bad day for us. And so for us to take the risk on our farm. For a biologicial pesticide, we had to have some data to really get us excited about it. [00:05:02] Overall, we wanna see performance. We need to see at least seven to 10 days. And I think that's maybe the biggest challenge a powerdy mildew issue is depending on what sort of climate and what variety of grapes you're growing is how long does it take me to get across the vineyard? [00:05:17] It's really what it comes down to. [00:05:18] And you know, maybe from a pathology point of view, Robert has some perspective. [00:05:24] Rob Blundell: The way we want to kind of think about powdery mildew is it's, you know, it's, it's always gonna be there. It's gonna be present. And biologicals, when used in the right way, can be a fantastic you know, tool in the arsenal. For, for growers or farmers against a deadly pathogen like this. [00:05:38] Growers really need to kind of consider the goal of using a biological, because there's so many different mechanisms of action of a biological, I mean, it can be live, it can be live, it can be the, you know, the spent fermentation product of a biological, which is gonna work very differently versus an actual liable organism you're gonna put in your field. [00:05:53] So kind of having a clear mindset from the, from the start is gonna be crucial to knowing. What kind of biological do you use? And also importantly, kind of when to use it as well. Because you can have drastically different outcomes based on like the time of your, you know, the time of venue production and then, and then the time of the season as well. [00:06:09] But yes yeah, ultimately there's broad, broad mechanism of actions. So if we're putting something on there live you know, you know, with something like powder mildew, this, pathogen functions because it attaches onto leaves. So we have these overwintering structures called cassia. [00:06:24] So these are basically the dormant structures that are gonna help powerdy mildew, survive. That's why it's been around for so long. That's why it's, it comes back every year. So it basically shuts down, it's fungal mycelium into these dormant hard structures. And then every year it basically reawakens around spring when we get the rainfall. [00:06:39] So we're gonna get ASCO spores. These are specialized spore structures within that kind of dormant structure. They get released out. So, you know, with the, with the weather coming in this week, that's gonna be, huge out there right now. So we're gonna get the release of those spores. [00:06:51] They're gonna land on that leaf. So really that's kind of our prime target of having protection is when they're gonna be landing and then adhesing to that leaf. So with something like a biological, if we can get that onto that leaf and then, you know, that's kind of our line of defense really. We want to be setting like a line of defense early in the season. [00:07:08] Know we have a product regalia. So that gets on there. It has these antimicrobial compounds, which the first point of contact is gonna. Prevent you know, it's gonna help mitigate that interaction between the leaf and the pathogen acts as kind of that medium layer. And then it's also gonna boost the plant's natural defense. [00:07:24] So how powdery mildew you kind of functions it. Once it gets on that leaf, it has a very specialized structure. Call it, they would call it a whole story or an appium, depending on where you are in the world and specialized structure that will kind of get through that cell wall, under that cell membrane and then sucks out the nutrients from the leaf so we can get a biological on the early to boost that plant defense, boost those, you know, defense fight hormone pathways. [00:07:46] We're gonna kind of mitigate that as a an initial point of contact. And then hopefully that's gonna set us off for a you know, a good season after that. But the time, yeah, the timing is definitely crucial. [00:07:55] Nevada Smith: I think to add to Robert's point is really to start your season off right and clean. So that's why as growers or as winemakers, you choose to use some sulfur to kind of mitigate, which is not necessarily a bio pesticide, but it could be organic, you know, depending on what your source of there. But those tools to me, are foundational for getting a clean start if you start bad, and it's gonna be a hell of a year all year long. [00:08:20] And I think that's the biggest challenge of bio pesticide uses overall is. Where do they fit, what growers they fit in? And it's not a solution for all, for sure. I mean, if you're growing Chardonnay or Pinot Noir on the Sonoma Coast in a foggy bank off of Bodega Bay, tough times, you know? But if you're in Pastor Robles, maybe in the Napa Valley in the valley where it's a little bit drier, you go in cab. Issue. You probably can integrate a nice bio pesticide program into it, and I think that's the secret. [00:08:58] Craig Macmillan: You mentioned regalia. What is the actual ingredient in regalia? What does it come from? [00:09:03] Rob Blundell: Yeah, so for Regalia the active ingredient comes from giant knotweed, so Ray Nectria. So that's a giant knotweed extract essentially that's been procured and then optimized in r and d and then applied typically as a folia spray for, for grape vines. [00:09:17] Craig Macmillan: And then the plant reacts to that, and that's what increases the plant defense mechanism. [00:09:22] Rob Blundell: Yeah, yeah, pretty much. There's kind of a few, few tiers of how, you know, Regilia kind of functions. So yeah, so we do that kind of initial application pretty much as soon as you, you have any green tissue, you know, really that's a great time to kind of get that on there. And then so the plant is gonna respond to that so typically a plant, defence pathway. [00:09:39] We have salicylic acid, so that is a key phyto hormones. So phyto hormones are kind of the driving force behind the plant defense. And this is very, you know, this is typical for all kind of pathogens, all kind of crops really. So you're gonna have a pathogen interact and we'll have its initial interaction with a plant. [00:09:55] And then you're gonna get this initial, like, response straight away from a plan. It's gonna be, Hey, I, my defenses are up. I, I sense this as a foreign agent. Basically I need to, you know, protect myself. So you get this upregulation of fighter hormones. They're very regulated. Pathways that then have these cascading effects to ultimately kind of therefore have longer term defense. [00:10:14] So you have an upregulation of fighter hormones. This is gonna signal to the plant that, Hey, I need to strengthen my cell walls, for example. So I'm gonna send more liening cell lignin being a crucial component a cell. wall . That's something we see upregulated as a result of regalia. So we get that increase in phyto hormones, we'll get lignin sent to the cell wall. [00:10:32] We get an increase in antioxidants as well to kinda help break down the pathogen as well. Limiteds effects we get polyphenols various other kind of antimicrobials as a result. So we have kind of direct effects, but then crucially with regalia, so we're gonna have the plant initially respond to its application, and then when the pathogen does. [00:10:50] Come around for a, an attack. That plan already kind of is, is heightened its responses, it's ready for it, so it's gonna be a faster kind of response time and therefore what we kind of consider more of a, a longer term defense response. [00:11:02] Craig Macmillan: Are there other modes of action, perhaps ones that are live? [00:11:05] Nevada Smith: Yeah. And that, I think that's a great point. Is there, you know, the, the bacillus category has been a big category the last dozen years or so. And this could be anything waiting from a bacillus subtles to bacillus Emli. There's other bacilli out there too. And I think they're more of an integrated approach. [00:11:22] So I conventional our farm vineyards. We're gonna just rotate it in there. So just like if you're straight organic or you're straight bio pesticide, it'd be a regalia, as an example, rotated with a bacillus product. We happen to have one as well, a very nice one called Sargus. But there's other great solutions out there in the marketplace today. There's other living organisms as well. There's some products in the Streptomyces categories as well. They're used in grow rotation, but I think to me as a grower and as a winemaker myself. I'm just looking for integration, IPM strategy all the way along. And depend on how, what your guard rails are for farming that would dictate what your options are overall. [00:12:07] Craig Macmillan: So, , to you, Robert, , how do these actually work? Like bacillus subtilis and things? [00:12:11] How do they actually either prevent or treat powdery mildew in grape. [00:12:15] Rob Blundell: Yeah, good question. So for Bacillus with Star in particular so we're actually not looking to treat powdery mildew kind of outright with this product itself. That's more where regalia is gonna come as a benefit. So actually Bacillus is great for something like botrytis in grapes. So, and this is really, really where we can kind of combine regalia and stargus together for a very effective program. [00:12:34] Kind of a one-two punch. So we, you have a live bacillus product. So we have spores that are gonna colonize a surface. So whether that's being the soil, you know, microbia the leaves or the berries, and with botrytis infecting berries causing damage, necrotic lesions in those berries, that's where something like stargus , a bacillus product can be applied to those berries to effectively colonize it. [00:12:55] And again, kind of creating like a nice. Kind of shield essentially from pretty much all fungal pathogens work the same. They have to attach, then they have to penetrate to essentially, hold on. So if we can kind of form a physical, kind of physical barrier, that's gonna be great. So for a lot of the Bacillus products they produce a suite of antimicrobials. [00:13:13] So star for our company we have a suite of antimicrobials that produces, so we have things like Itur, Phin, these are all really good antimicrobials. They're gonna have a direct effect on it. So those spores will be able to, you know, colonize the berry, for example, and then help Yeah. Prevent prevent powerdy mildew So you have this live culture essentially that's on the grapes and it's producing compounds, and that's where the, the antimicrobial comes in or the antifungal comes in. [00:13:40] Nevada Smith: Yes. And. [00:13:47] So there's two registrations from an EPA standpoint. There's the live bacteria count, which people are familiar with from back in the day when there was bts, right cells ths for worm protection. And so we measure the CFUs, which is a colony forming unit. So the bacteria, and there's a minimum threshold that we have for our product as well as anybody else that registers their bacteria. Just sort of a quality control thing for the grower to know this is the level we produce. What we. Seeing the production for our solution is really around the chemical compounds being created in the fermentation process, this lipopeptides cycle. And so that's what's important to know that there's some differentiation. [00:14:25] And I always use the example, I'm a huge basketball fan and you know, there's a difference between Michael Jordan and myself. I'm not at his level. And so not all bacilli are created equal, but they all do have some performance values for them. And obviously, you know, the more you can look into science and whether it be uc, extension and the Gubler Eskalen models and local trial researchers will give you the value proposition each of these products brings to you. [00:14:50] Craig Macmillan: Now, this is something that I, I don't think I've heard before and I wanna make sure that I heard it correctly. So, some of the protection is actually coming from things that are being produced during the fermentation production of the bacteria themselves. And so these are side things. And then that makes it into the final product. [00:15:05] Nevada Smith: Yeah, that's actually the most important thing on foliar. So holistically for bacillus, and this is a very broad brush here unless you're in a tropical environment like bananas in. Columbia or Costa Rica, you're not growing more spores on the leaf surface. You might have that happen a little bit depending on sort of your micro environments. What you really want is coverage and then that eradicates. [00:15:29] The way that the the bacillus really works, it really pokes holes into the cell wall of power mildew. So that's, and it just kinda leaks out and dies. And so it's botrytis , and or powder mildew. That's the major effects that it has on these pest diseases. [00:15:43] But in those rare examples, I'll tell you, we've seen some results of our products being used in crops and tropical environments. If it can grow, it's creating more value. Now let's talk about something different. You put bacillus. Sargus into the ground in a soil treatment. It has tremendous effects on colonizing around the roots. [00:16:01] And so that's where bacillus is actually known in its natural environment into the soil profile. So that's where we really see that the one two value. Now, that's not what we're using it for in grapes. Grapes, is for foliar control of. And mild diseases. But we have many other crops that we use bacillus for like corn, for root management and prolification around the diseases down there. [00:16:27] Craig Macmillan: Do you have anything to add to that, Robert?  [00:16:29] Rob Blundell: Yeah, so that's, yeah, excellent points from Nevada. So yeah, kind, kind of getting, talking about how we can use bacillus, you know, actually to go into the soil. So something like nematodes, you know, that's, that's a huge issue in grapes always has been. It's where we have, you know, root stocks engineered over the years to have, you know, nematode resistant root stocks. [00:16:43] Again, not, not kind of the primary purpose of what we'd be looking to use stargus, and vineyards, but again, having a soil colonizer is fantastic. You know, a lot of the. The majority of diseases, especially in like the row crops, they're coming from the below ground. You know, you've got the pythium and lettuce. [00:16:57] You've got like sclero, things like that, huge kind of soil-borne pathogens. So again, having something that you can add to the soil, you know, the soil already has its own fantastic suite of, naturally present. You know, bacteria, fungi, that's, you know, like Nevada said, that's what we got ab baus from, stargus from. [00:17:12] So we're just kind of adding to that to kind of help boost the fight. And we can always kind of think of the interaction between pathogens and plants as kind of this arms race. There's a ways, you know, the pathogen kind of gets ahead by evolving slightly, and then you have the ho response from the plant and then the, the microbiome as well. [00:17:27] So we're just trying to kind of tip the scales and our balance is how a good way to kind of think of biologicals as well. And I think as you were mentioning, kind of the, the fermentation process, and that's where we get our microbials from. [00:17:37] Every microbe has primary metabolites. That's what's key to basically the survival of a microbe. But then we have secondary metabolites, and these are very highly specialized products that get produced. For bacillus, during that fermentation process, this is a, you know, these are unique metabolites. You know, metabolites are produced by the majority of. Micros, but the in particular can produce these like fantastic suite of very unique metabolites. So that's where the, a non-life product kind of comes into itself as well. By us able to understand what are those metabolites we're producing same fermentation, can we optimize those? And then do we, do we even need a live product as a result of that? [00:18:12] Craig Macmillan: Um, it sounds like this could have a really dramatic impact or role in fungicide resistance management. I. What is that role? Or are we talking about going over completely to biological for a program or are we including in a rotation with other materials? What about organic growing where we have a, a little smaller suite of things that we can use? [00:18:35] Nevada Smith: , I'll start with that if you don't mind. [00:18:36] I think it's a great question and where I see it fitting is most synthetic pesticides for disease control are really affecting the mitochondria on the inside of the dupo. And where I see it fitting is the sort of one, two, I would say contact plus systemic. That's an a de-risk, your resistance management issues. But B, increase the likelihood that those products work better and longer. [00:19:02] So today we position a product like Sargus other bacillus products in the marketplace to be in combination with a. SDHI chemistry, like Luna would be an example of that, or Pristine. We would see those integrated in the cycle of sprays, which is, it's very similar to why you use sulfur with those products as well. [00:19:23] But I think, you know, as a winemaker, I want less sulfur my crop as possible, but obviously I want, as a farmer too, I want it to be clean as can be. So it's kind of this yin and yang overall. [00:19:33] But for resistance management, I think you have to really think about the whole approach. And once again, back guardrails. Of what your restrictions are for you as a farmer and maybe the winemaker working together with them. How do you really get to the. And, you know, I, it's kind of a joke too, but we talked about earlier the word sustainability be very broad. Stroke. Well, I'm wanna farm into the future years. I wanna have that vineyard for a hundred years and not to replant it. So I'm really trying to keep as clean as possible all the time, especially for the over wintering stuff. And so to me early often protection, control contact plus systemic is the approach that we take at our farm as well. [00:20:10] Craig Macmillan: When we say earlier, are we talking bud break, two inches, four leaves?   [00:20:15] Nevada Smith: For powder. Yeah. But then we could debate, you know, on these opsis issues and can cane issues. [00:20:24] Craig Macmillan: When would I wanna put on a bacillus? [00:20:27] Nevada Smith: I would start with a sulfur spray about bud break here, and then kind of rotate back into the bloom time for the first bloom spray, about 50% bloom, more or less. I kind of time it too, and if it's a little later, I'm okay with that. That would be the major time where I get the first shots on and that we, I would start with regalia, for example, just because it's a different mode of action. And then I'd come back with the bacillus here about seven to 10 days later. [00:20:51] Craig Macmillan: And would you then include synthetic materials as well, I'm assuming. [00:20:55] Nevada Smith: Yeah, on our farm we would typically our biggest issue is getting across the, the vineyard. And so we're looking to start off with a synthetic material first, just so we can get a nice, well, sulfur first, sorry. That probably like A-S-D-H-I chemistry. And then I'd start to think about how can I integrate my approaches to, being softer chemistry based through the rest of the season. [00:21:17] Craig Macmillan: Does that make sense to you, Robert? [00:21:19] Rob Blundell: Yes. And actually I'm just gonna jump back a little bit in our conversation. I just add a few more details kind of on this approach as well. So yeah, a little bit earlier, I kinda mentioned this arms race between the pathogen and the host and, you know, the available treatments that we have and really kind of a huge benefit of. Adding a biological, say, into your conventional program or just introducing more biologicals in general for your, your fungicides is you know, as, as Nata was saying, you know, a lot of the conventional chemistry is targeted in that mitochondria. It's a very specialized kind of function. It's there, it does a great job when it works well, but then. [00:21:51] We get pathogen resistance, obviously. So there's kind of two types of resistance. You get qualitative resistance and quantitative. So qualitative is when there is a kind of sudden or abrupt loss in the ability of say, a fungicide to work. And then you have quantitative where it's kind of more of a gradual decline in effectiveness. [00:22:08] And then you get kind of these varying levels of fungicide sensitivity versus that qualitative where you're having either resistant or a sensitive is isolate. And this. It's great. We're talking about grapes and powerdy mildew, 'cause this is one of like, this is like the classic textbook example. We kind of get taught in pathology about this because powerdy mildew, it has these really quick cycling times, produces a number of generations per season, very easily dispersed. [00:22:28] So this is such a high risk kind of category for this fungicide resistance. So again, if we have just a whole range of availabilities in terms of different fungicide options, you know, chemistry, soft chemistry, biologicals various other options, we're just kind of increasing our chances of really. Just well, and one not having any pathogen resistance. [00:22:49] Because again, as soon as you have that, then you have you, you really lose your options for your chemistries. So again, just, you know, introducing a few biologicals here and there, especially for, you know, grapes on the West coast, which is the amount of sprays we're having to do in other states where you have less sprays, you can kind of get away with kind of not considering your approach a little bit more. [00:23:05] You don't have to kind of. Do your frack checks as much because maybe you're only doing one or two sprays. But here we have to be very, very concerned with our, you know, what products we're using and then at what timing they're using. So again, just having a biological to really kind of take the pressure off some of those chemistries is a, is a huge a huge, valuable source of preserving the life of your chemistry. [00:23:23] And then have, like Nevada said, you know, having sustainable wines for the years to come. [00:23:28] Craig Macmillan: Actually, that made me think of something. Is there a risk of resistance being developed to biological strategies? [00:23:38] Rob Blundell: Yeah, that's, that's a really good question. So yes. [00:23:41] It's kind of a newer question. Yeah. So again, with a lot of these chemistries being very, very site specific function, all you have to do is have a very small mutation in your, say, powerdy mildew, to overcome that. And typically with biologicals, the typically, I say typically the mode of action is a little bit more broad. [00:23:57] So very rarely are you gonna have an extremely like. , so like a lot of the chemistries buy into certain receptors that their job that do that really well. Biologicals don't tend to do that as much. They're more of a broad spectrum. That's why we see a, like for our fungicides, we see a range of control against a lot of different, you know, powerd mildew, we've got ascomiscies,, Presidio, my seeds, they pretty much do well across a range because they are more broad spectrum. [00:24:19] Not to say that in time we're gonna start to see a decline. It's, you know, again, it's kind of really how we consider using them. And we. Whether we wanna like, fully rely on them or hey, that's, let's, let's use more of a, a combined approach. So again, we just really make that sustainable as well. [00:24:33] So kind of to answer your question definitely it comes with risk but kind of inherently due to the more broad spectrum nature of biologicals, we're not too worried about the kind of resistance that we've seen developed as a result of c chemistries in that very, very specific function of a chemistry. [00:24:48] Craig Macmillan: That makes a lot of sense. I know that you had mentioned you're farming in a more traditional fashion, Nevada, but your products, and obviously I know some folks in the organic area. What role do biologicals play in an organic fungicide program? Nevada? [00:25:03] Nevada Smith: I think it's definitely at the core of your foundation of seeing how you are gonna approach powerdy, mildew and botrytus. Is it a typical, you know, seven spray system, which I'd say it's kind of typical for the northern coast markets or the coastal range. Or if you're in the valley floor are you more in that three to five applications for bio pesticides and, and what timing and how you're approaching those things are critical overall to assessing those on the organic. [00:25:30] You don't have to be just organic. You could be, from a theoretical point of view, you can just choose to be this type of farmer, which is, I want to choose softer chemistries. And I think that's the mixed bag that we deal with with customers, a crop and the crop advisors out there. [00:25:44] Rob Blundell: Yeah, and I was gonna say just to kinda add to that as well. So again, regardless whether you're doing organic or chemistry or biologicals, you know. Really key as well. Foundation is just having good cultural control as well. Something we haven't really touched on today, but again, you can really increase the effectiveness of your biological, your chemistry based on what you're doing in, in the vineyard. [00:26:02] So, you know, things like, you know, canopy thinning, so if you're using say, a biological, you wanna try to colonize those berries, you wanna kind of thin out that kind of piece. You're getting a better spray coverage. You're also gonna, you know, reduce the humidity and that kind of pee of things like mildew you know, effective pruning in dry conditions. [00:26:18] Navar was kind of talking about opsis, some of those canker pathogens. So those grapevine trunk diseases, that is still the most effective way to control a grapevine trunk disease is just to prune under the right conditions. 'cause you need that wound, that pruning wound to heal when it's, you're not gonna get a, let's see, you know, we got that ring coming in this week. [00:26:33] So, grapevine trunk disease is dormant on those on the, on the parts of the vine. They're gonna be airborne. So you need to make sure there's a very good dry window. So again, like cultural practice is always, always key to whatever approach or biologicals or chemicals. [00:26:46] Nevada Smith: I think the add to that, one of the biggest things I remember, I wanna say it's like in 2010, I saw Gubler trials, Gubler, uc, Davis, you know, famous for everything. And he had the trial and all he did was pull leaves. On the bunch closures, and I was like, wow, that looked amazing. And I said, what? What spray did you have on there? [00:27:02] And they're like, nothing. We just pulled leaves and just literally that airflow coming across there, drying out, I assume it was just drying out the spores was amazing. I was like, wow. But then I started doing the cost analysis as a grower. I'm like, I can't send a crew there and pull leaves all the time. So, [00:27:19] Craig Macmillan: Yeah, it's true. I mean, and that's why it's a mix of things. I think. It's integrated pest management. You, you know, you do want to get some airflow through there. You will probably do some canopy management, whether you do shoot thinning or leaf removal. Some of that also helps with coverage. [00:27:32] Right. So using a mix of cultural and chemical or pesticide techniques is probably, probably wise. I'm not a pest control advisor, so I probably shouldn't say that. I. But I think I, you, they're not the first folks that have, have reminded me of that. And sometimes I know that, I think we kind of forget. [00:27:49] I wanna change topics a little bit. There's a, I don't wanna say new, but new to me. Area bio fertilizers a totally different kind of strategy for plant nutrition Nevada. What is a bio fertilizer? What, how do they work? What is it and how does it work? [00:28:05] Nevada Smith: So bio fertilizers can be a multitude of things, but once again, back to bio based on living organisms prior living organisms. We happen to have one that we're just launching this year into the grape industry called Illustra. It's based on this unique technology, UBP. Universal biological platform. I'm not trying to be a billboard ad here, but the reason why I'm bringing it up is it, it's really is a platform, which is interesting about it because it's, it's a technology that we can change and manipulate depending on how we go through the production cycle. And so we're creating tools that are more made for abiotic stresses. [00:28:39] And so we're trying to deal with different stresses that. Crop can deal with. And so right now the core market that we've been using these products , for is like soybeans and corn. [00:28:49] But as we think about the permanent crop markets of grapes, tree nuts, citrus, it's a little bit different as far as cycle and how you approach it. And so what we've seen through the data, these bio fertilizers is really trying to mitigate abiotic stresses. So what we're really mitigating is one, like you, you think about herbicide applications. You kind do a banded application near the tree trunk into about a third of the spray row. That herbicide usually hits that tree trunk. [00:29:14] There is a cause and effect on the grapevine itself. What if you could put a tool down that was sprayed on the same time to mitigate that stress or de-stress it from even how much time and pressure it's having? So. Our product is really one of those tools today that's really focused on mitigating biotic stresses. [00:29:30] Other things I can think about as a farmer is like salinity in the soil. The roots are pushing. You have water issues in California. We all talk about that. How do you mitigate the plant that still maximize the yield? So. Choosing the bio fertilizer today that's really focused on that, not just being a typical, you know, can 17 or un 30 twos based nitrogen based products. [00:29:51] This is something else to bring into the marketplace. They're kind of more niche based, depending on what you're dealing with. But there there's several out there. There's, seaweed extracts would be a big one, right? That people use a lot around farms. There's humic, andic acids, organic acids in general. So those are the kind of the buckets of items today that farmers are choosing for bio fertilizers. [00:30:14] Rob Blundell: Hmm. Yeah. And I can yeah, touch a little bit more on the, on the UBP illustrate product as well in terms of kind of how, how that really functions. And as Navar said, it's, you know, helping bounce back after, say, some herbicide damage, promoting that early season boost in biomass. [00:30:27] So, you know, a product like this, this UBP will basically kind of. Inducing cell division. So in you know, increasing mitochondrial activity, more cell division essentially leads to more chlorophyll, more photosynthesis graded by a mass production. And it's actually done by acidifying the cell wall. So we acidify a cell wall. You get more what we have these, there's proton pumps on these cell wall. [00:30:48] We're basically pumping in more protons, increasing the rate of that cell division. So we're basically yeah, boosting that in ocean season biomass. Therefore having that. You know, quicker resilience to say, you know, abiotic stresses like no said, whether it's salinity, salt, drought, water, things like that. [00:31:02] So yeah, numerous, numerous benefits of some of these fertilizers. [00:31:07] Craig Macmillan: Which actually talking about antibiotic stress, that it reminds me of something. I want to apply it to this, but I also want to go back. If you're using a live material, a bacillus or something, or if you have a, a bio fertilizer that may is are there living things in bio fertilizers. [00:31:22] Nevada Smith: There can be, [00:31:24] uh [00:31:24] Craig Macmillan: be. Okay. [00:31:25] Nevada Smith: We don't have anything in ours today, but I think there are, let's call the word impregnated Fertilizers. With living organisms. It could be trico, dermas, it could be other things, bacillus. And those are good, good tools to use. [00:31:39] The hard part is like, you know, now we start to open the can of worms around like compost tea, like what's in there. And I think that's the biggest challenge that growers, those things do work as a whole. But then you start to run into the quality assurance, quality control. And I think that's where companies invest in the bio pesticide industry are really trying to. Tell the story and not just be perceived as snake oils and saying, Hey, replicated work we measure to this level, like CFU content and here's what we expect results to be consistently. [00:32:08] And this is sort of the shelf life issues and we're kind of getting as a, you know, the world evolves. I think there's just this environmental things that people choose to do. And I think, you know, everything works. Just a question of how you integrate it into your own farming systems. [00:32:24] Craig Macmillan: So speaking of environmental factors and antibiotic stress one thing that's occurred to me is that if I have something that's that's out there, either that's living or maybe maybe a fragile compound, how do things like drought and heat affect these materials in the field? [00:32:38] Rob Blundell: Yeah. Yeah, very good question. I think historically that was always kind of. What people thought of the negative of biologicals were like, well, is only gonna work under certain conditions. You know, where, where have you tested it? So yeah, it's, it's a good question as well. [00:32:50] It's , case by case dependent you know, certain extremes and temperatures, various conditions as well are gonna have effects on, you know, the, the longevity of that. But we, you know, we try to test it under. There a variety of conditions. And then for particularly something you know, with our fungicides as well for, for the grape industry, you know, these new be tested on a variety of key varietals as well. [00:33:10] You know, it's, Hey, it might work for Chardonnay but not for Sauvignon Blanc. So that's important to evaluate as well, rather than just bring a product to market that like you, it's only gonna work on very certain aspects of a, of the single industry. [00:33:22] Craig Macmillan: So heat as an example, , you have a fair amount of confidence that I can apply something in the, in the heat if I have a hot, dry condition in the summer that it's not going to. Break down those materials that are there from the fermentation or kill the live organism. We, we think there's a fair amount of resilience here. [00:33:39] Rob Blundell: Yeah, again, definitely gonna be dependent on the, the type of microbe and the type of metabolite that it's producing. But you know, microbes in nature are exposed to these extreme conditions just naturally anyway, you know, so we have epi amplified slipping on the surface of products. So on the surface of. [00:33:54] Structures. So like a grapevine, like a leaf. They're obviously out there and exposed to the elements every single day. And then the soil is a, is a chaotic environment. There's a lot going on in the soil. So microbes are just, you know, extremely resilient in nature themselves. So there's gonna be a, again it's gonna vary depending on, you know, the microbe and, and the product we're using. [00:34:12] But there's good efficacy. [00:34:16] Craig Macmillan: What's the future? What is the future looking like for biological products, living or extra? [00:34:23] Nevada Smith: for the marketing hat on myself, not the farmer side. [00:34:27] It, I think everything's coming down to specialized sprays. And if I had to vision what the features look like to me, it's gonna be about. Seeing robots down the vineyard. They have 18 different things and their little mechanisms and there's, they're just, they're analogizing what's going on in that grape cluster itself. [00:34:44] They're spot spraying three or four things and they're going down the next level. That to me, is where we're gonna get down to the future, where the grapes themselves will naturally grow less chemicals to be used overall. [00:34:54] but if you need to go through and really take care of a problem, you're gonna go through and take care of a problem. And I think that's where it's become very exciting to me. You're gonna put less of a prophylactic spray across all systems, and you're kind of really create some microenvironments where you think that Vine number seven got sprayed a lot. Vine number 21 has not been sprayed all season. Wonder why? Let's go check it out. Let's understand and investigate. [00:35:18] The other big thing I think in grapes that's really interesting from exploratory research and development side for our company is like viruses. Viruses have not been addressed and it's becoming an issue. It's something I want to kind of explore and put on our docket of, you know, assessment stuff and how we can take new technologies to really improve virus transmissions. How do you mitigate once you have a virus? And it still produce that vine for another 10 plus years. So it gets quality and quantity out of it. Those are the kind of things interesting to me. [00:35:50] Craig Macmillan: Robert. [00:35:51] Rob Blundell: Yeah, definitely. Yeah, really good point, Sarah as well. And yeah, viruses in particular is, is something we see about in the grapevine industry. And yeah, often biological companies we're focused on, you know, the, the fungal issues, the bacteria, the, the nematodes. So that's, that's a huge area that really needs some more dedication. [00:36:06] So there's gonna be some great technologies available for that in the future. Yeah, I think to speak to no Nevada's points on kind of the future of it, I think like a really kind of custom tailored approach is gonna be available for those that want it. Particularly from the pathology side of my interest. [00:36:19] I think precision monitoring and detection of disease is just, I. Advancing leaps and bounds. So again, like, you know, going out there and doing scouting, hopefully people are gonna have a lot better tools available, available to 'em in the near future to really kind of understand crucial times in their season where disease is coming in. [00:36:36] And then again, like I. Just having better tools to kind of really actually di inform us of the pathogen as well that's present rather than just again, a lot of, a lot of diseases is hard to pinpoint to an exact pathogen. We're lucky in grapes, powerdy, mildew, and, botrytis are very obvious. We know what those are, we think are some of the row crops. [00:36:52] It could be a whole host of things. We've got nematodes, we've got various sore pathogens that we can't actually see. So I think yeah, improving disease diagnosis and detection, having these precision tools is gonna be a huge part of the future where biologicals can integrate themselves in as well. [00:37:07] Craig Macmillan: That sounds pretty exciting. I wanna thank you both for being on the program. This has been a really great conversation. My guests today we're Nevada Smith. He is the head of Marketing North America and Robert Blande, who's a research plant pathologist, both with Pro Farm Group. Thanks for being on the podcast. [00:37:22] Nevada Smith: Appreciate you. [00:37:23] Rob Blundell: Thank you very much, Craig. It was a pleasure. [00:37:25] Craig Macmillan: And to our listeners, thank you for listening to Sustainable Wine Growing Vineyard team. [00:37:29] Nevada Smith: Craig, one more thing. We gotta just drink more wine.  [00:37:40] Beth Vukmanic: Thank you for listening. [00:37:41] Today's podcast was brought to you by Vineyard Industry Products serving the needs of growers since 1979. Vineyard industry products believes that integrity is vital to building long-term customer, employee, and vendor relationships. And they work hard to provide quality products at the best prices they can find. Vineyard industry products gives back investing in both the community and the industry. [00:38:06] Make sure you check out the show notes for links to Pro Farm, an article titled, what are Bio Pesticides Plus Related Sustainable Wine Growing Podcast episodes. 117 Grapevine Mildew Control with UV Light 123. What's happening in biologicals for pest management and plant health? 266 Soft pesticide trial for powdery mildew, downy mildew, botrytis and sour rot, and a healthy soils playlist. [00:38:34] If you'd like the show, do us a big favor by sharing it with a friend, subscribing and leaving us a review. You can find all of the podcasts on vineyard team.org/podcast, and you can reach us at podcast@vineyardteam.org. Until next time, this is Sustainable Wine Growing with the Vineyard team.   Nearly perfect transcription by Descript

To understand fungicide resistance in the vineyard, a group of United States scientists formed the Fungicide Resistance Assessment Mitigation and Extension (FRAME). Sarah Lowder, Assistant Professor and Viticulture Extension Specialist at the University of Georgia describes a nationwide sampling project to test for resistance markers. To get samples, they are promoting a new collection method called glove sampling. This process leverages the time that fieldworkers are in the field running their hands through the vines. They collect samples by rubbing their gloved hands on a cotton swab and sending the sample to the lab. Research shows that glove sampling results are very similar to spore trapping, a process that samples the air flowing through the vineyard. Resources:         117: Grapevine Mildew Control with UV Light 219: Intelligent Sprayers to Improve Fungicide Applications and Save Money A Rapid Glove-Based Inoculum Sampling Technique to Monitor Erysiphe necator in Commercial Vineyard Fisherbrand™ Plastic Handled Cotton Swabs and Applicators Grape FRAME Networks Glove swab sampling tutorial for collecting grape powdery mildew (video) Glove Swab sampling tutorial for collecting grape powdery mildew - silent (video) Identification of Putative SDHI Target Site Mutations in the SDHB, SDHC, and SDHD Subunits of the Grape Powdery Mildew Pathogen Erysiphe necator Rapid sampling technique to monitor Erysiphe necator more effective than visual scouting Sarah Lowder Vineyard Team Programs: Juan Nevarez Memorial Scholarship - Donate SIP Certified – Show your care for the people and planet   Sustainable Ag Expo – The premiere winegrowing event of the year Sustainable Winegrowing On-Demand (Western SARE) – Learn at your own pace Vineyard Team – Become a Member Get More Subscribe wherever you listen so you never miss an episode on the latest science and research with the Sustainable Winegrowing Podcast. Since 1994, Vineyard Team has been your resource for workshops and field demonstrations, research, and events dedicated to the stewardship of our natural resources. Learn more at www.vineyardteam.org.   Transcript 2024-07-04_235_Sarah Lowder - glove sampling for mildew_Otter [00:00:00] Craig Macmillan: Our guest today is Sarah Lawder. She is Viticulture Extension Specialist and an Assistant Professor in the Department of Horticulture at the University of Georgia. And today we're going to talk about some exciting new advancements around monitoring for powdery mildew and other related topics. Welcome to the podcast, Sarah. [00:00:16] Sarah Lowder: Thank you very much, Craig. I'm very excited to be here. [00:00:18] Craig Macmillan: First though, I want to start with something I just found out about that applies to this, and that is the grape frame networks. Can you tell us exactly what that is and kind of how it came about and what it does? Okay. Thanks, Craig. [00:00:29] Sarah Lowder: Absolutely. Yeah, the FRAME Network is part of a USDA SCRI grant. This is a project that was funded several years ago, I believe in 2017, as part of the Specialty Crop Research Initiative Project System. And FRAME Networks literally stands for Fungicide Resistance Assessment Mitigation and Extension. networks. And so this was a grant that was really intended to look and learn so much more about how fungicide resistance works in the vineyard for powdery mildew specifically. But this was a group, a very large group of scientists from all across the country from a wide range of different disciplines looking into how we can better address fungicide resistance for vineyards and for powdery mildew specifically. [00:01:12] Craig Macmillan: But there's also applications either now or in the future for other fungal diseases like downy mildew, et cetera. [00:01:18] Sarah Lowder: Absolutely. Yep. They are currently working on getting a new iteration of this grant, Frame 2 as it were that will hopefully be able to address also resistance in Downy Mildew and Botrytis Bunch Rot. [00:01:29] Craig Macmillan: Fantastic. This is really exciting because what I saw was that there are people at Washington State, Oregon State in California, and then on the East Coast. So hopefully this work will continue. What exactly kinds of things does Frame do? [00:01:45] Sarah Lowder: Yeah, so we had a multi pronged approach for this project. One of the big parts that I was a part of is looking at the assessment of the fungicide resistance. So one of the things that we did was collect powdery mildew from all across the country, from states and vineyards all over, all over the place, and then look for different fungicide resistance markers in that mildew. That can hopefully help us much more quickly assess if we have fungicide resistance present in a sample. One of the big groups of fungicides that we looked at was the QOI fungicides, the Sturbulurins, also sometimes they're called or the FRAC Group 11, with FRAC is the Fungicide Resistance Action Committee. Is what the FRAC group would be for that and those just kind of group your different fungicides based on the mode of action. So how they work on the different diseases that they're attempting to control. And so we were looking at this group, this group 11, the QOI products which operate by attaching to the mitochondria, so the powerhouse of the cell. And just preventing them from being able to produce energy and so the, the spores die and you don't get any more growth of that disease. But because it's one of those products that works like a binding site if you get resistance presence it just means that your fungicide can no longer attached to the disease and then it can grow in the presence of that fungicide and then you can even no matter how much you spray that product it can still continue to grow. By looking at there's one particular mutation that occurs that causes that resistance in this group of products and we're able to run a test much more quickly than you can otherwise do a lot of different fungicide resistance testing And tell whether or not you had QOI resistant or sensitive Mildew in your field so you knew whether or not you could use that product or not [00:03:35] Craig Macmillan: What about frac group three? That's another one that we have known Resistance issues with the demethylation inhibitors. Is that part of the project as well? [00:03:43] Sarah Lowder: Absolutely Absolutely, the DMIs demethylation inhibitors, absolutely, the FRAC3 Group 3 are a group of products that have a little bit more complicated unfortunately of a resistance pattern rather than just having one particular mutation. There, there are a bunch of different things going on in those products. But we do have a marker that can help us get a sense for the resistance. It's not as necessarily reliable as the QOI. Frack Group 11 product resistant testing, but we can test that as well and give us an idea of whether or not we might have resistance to DMIs present in the field as well. [00:04:19] Craig Macmillan: Are there other frack groups that have potential or have found some resistant populations coming down the pike? [00:04:28] Sarah Lowder: Absolutely. Anything that has a very specific mode of action really has some potential for having a resistance develop in a population. [00:04:36] Craig Macmillan: Great. [00:04:38] Sarah Lowder: We're, we're looking at some of the modes of action. We have not heard as much of field failures due to resistance to a lot of other products, but we wanted to, like, kind of get a, get a jump on looking for some of those other modes of action to see if we can develop. Test to be able to monitor that, monitor that much more quickly before resistance becomes as much of an issue. And we were able to get a lot of that information for like the SDHI groups, which is the group 7 products. Although again, we're not really hearing too much yet of any field failures for powdery mildew control from the SDHIs. As well as looking much more into some of the mechanisms for how group 13, quinoxafen how well how the resistance might work in that system. All of those are stuff that we were able to do because of the Frame Network project team. [00:05:26] Craig Macmillan: That's really great. I hope that we can continue that. That's really important work. In order to do that kind of thing, you need to get samples. And you and your colleagues are promoting a new method for monitoring for powdery mildew and collecting, I guess, ASCA spores, I would imagine, is what you're collecting. Tell me about it. There's videos on YouTube. It's really exciting. [00:05:47] Sarah Lowder: Absolutely. [00:05:47] Craig Macmillan: It involves a glove. [00:05:49] Sarah Lowder: Indeed, we call them glove swabs, which is one of the projects that I was able to help lead as a part of the system. Because you hit the nail on the head when you are trying to monitor fungicide resistance. The first step that you have to do is actually find the mildew. And you want to find this before you have a control problem. You want to find it while there's still not very much mildew present in the field. We looked at, we looked at a bunch of different ways. Not everything worked very well. But one of the ideas that we had was, oh, We have vineyard workers moving throughout the field all the time. Could we potentially use these people who are already in the field doing normal stuff to also potentially collect powdery mildew? And we found that as you're moving through the vineyard, as you're moving your hands along the canopy, you're able to collect powdery mildew. Conidia spores, actually, it's generally, it's their summer spores. are collecting and you're able to take a, just a cotton swab. So many of us have taken a COVID test that you've had to stick up your nose. It's a very similar, similar swab that you take with your hands. And you can stick that in a tube and send it to the lab for processing. This system worked, I think, way better than any of us were expecting it to. It was actually much better than trying to go out there and look for it physically. And you can do it much faster because it's just literally, you can be walking through the vineyard with your hands in the canopy, take a swab. and collect it, and you can find that so much faster if you're already having to do a lab test for that product. [00:07:16] Craig Macmillan: And also the potential here is that, let's say I've got a crew out and we're doing a shoot thinning in the spring, so they're handling the canopy all the time, we could swab some gloves and then send that out to be tested and get an idea what's out there. [00:07:27] Sarah Lowder: Absolutely. Absolutely. And as you're already testing for whether the field is out there, you can also use the same test to tell whether or not you have resistant mildew to the QI fungicides. [00:07:38] Craig Macmillan: Is this quantitative? [00:07:41] Sarah Lowder: So, it is run on a quantitative quantitative PCR. So, in theory, it does give you some information on how much mildew is present. In the QI QPCR test, in the test that tests for the fungicide resistance pattern, it tests a piece of DNA in the mitochondria, and you can technically have different numbers of things. Mitochondria per cell, individual cell, and so the, the quantitative, it does give you that information, but it's not necessarily the most reliable information. So as, as a scientist, I'm always hesitant to say that, oh, you can get quantitative information, but it, in theory, it does give you a little bit of information about how many spores at least you were able to collect. You can tell a little bit of that information. [00:08:21] Craig Macmillan: Can it tell me something along the lines of, I can go out and sample at the very beginning of the season, maybe even post bud break, and say, oh, we have nothing there yet, and then come by later and test and say, oh, we have something there now. Is there a qualitative aspect to it? [00:08:41] Sarah Lowder: Absolutely. So we tested it and compared the glove swabs to visual scouting to compare it to someone actually physically going out and looking and found that the glove swabs were much more sensitive. They were able to find the powdered mildew much faster, and also much sooner than the OR than our visual eyes were able to do So. But we also tested it in comparison to impaction spore traps. So these are tests that actually had come out of a lot of the lab that I worked in the foliar pathology lab with Walt Mahaffey at the USDA for a couple years previously with like Lindsay Thiessen that they were able to test these impaction spore traps which sample the air that flows through the vineyard. We're collecting that many of the vineyards in the Willamette Valley of Oregon which we did a lot of the testing in that area as well as in Areas like Napa, California have a bunch of these spore traps present that we were able to test it in comparison to these spore traps, which have been used pretty widely in the system. And what we found was that our glove swabs were showing us very similar information to these impaction spore traps which was really cool because a lot of these growers absolutely do use already these impaction spore traps to look at when they're going to initiate their fungicide spray programs. So, in theory, yes. You could absolutely potentially use these glove swabs as a fungicide initiation indicator. [00:10:01] Craig Macmillan: Yeah, that's what I was getting at, and that's, that's a really exciting technology. Because obviously we're trying to control the amount of material we put out there, and obviously part of resistance management is being careful what you put out and when. And so having a good idea of what the timing should be, I think, is really important. And then of course, as everybody knows, People who listen to this podcast know, by the time you see it, it's too late, baby. [00:10:24] Sarah Lowder: Absolutely. [00:10:24] Craig Macmillan: You know, it's, it's, it's, you're chasing a ghost. And so, here's, here's our Ghostbuster tool, where we can catch that ghost before before it comes too much of a problem. Now you said, send these off for, to a lab for analysis. What what labs are we talking about? [00:10:39] Sarah Lowder: The initial frame grant project is technically at a, has come to an end and so the funding that originally funded a lot of this testing has ended, but now we're able to supply all of these protocols and testing materials and stuff to a lot of the different private labs elsewhere. So I know one of the private labs that does the impaction trap testing. service in the William Valley of Oregon has taken over that process, as well as I know a bunch of different labs like I know at UGA here, where I am currently, they have some tools and able to do some of those testings or do some of those tests as well with the qPCR. And so even if you, a lab where you send a lot of your materials, if they happen to have a qPCR present and you're strongly interested in doing it, you can talk to them to see if they might potentially be willing to add this into their repertoire. [00:11:24] Craig Macmillan: So, this technology is starting to make it out into the world of commercial plant pathology. [00:11:29] Sarah Lowder: Yes. [00:11:30] Craig Macmillan: I may have a plant pathology lab that I already work with, I can talk to them, I can make some phone calls, and for instance, a commercial lab in Oregon, they're a business, so they still may do samples from California or from Washington or from Ohio. [00:11:44] Sarah Lowder: Yeah, potentially. [00:11:45] Craig Macmillan: It's not limited by territory. [00:11:46] Sarah Lowder: Correct. You can send these samples and as long as you're not leaving your samples out in the sun and in the heat for a long period of time they can be shipped off elsewhere as well. [00:11:55] Craig Macmillan: Where do you see this technology going? What's, what's the future here? We, we, we've just kind of touched on it a little bit, but what, in your, when you close your eyes and imagine the long term impact of your work, where do you see this kind of thing going? [00:12:08] Sarah Lowder: One, I would love to see a lot of this information being used more regularly to be able to test this a little faster. And eventually, one day, we may be able to test for all of these different resistance genes, all of these different resistance patterns within the same sample. So you could be like, okay as a normal part of my practice, I'm going out and taking these glove swabs or whatever else that I'm looking to test. Even if I don't use a glove swab, but I can come in and take my powdery mildew sample and send it off to the lab and get a, just a score sheet of exactly what fungicide products could be used or could be not used. That would be really awesome, because no one wants to buy a product that it's not going to work, and no one wants to put a product out that's not going to work for any reasons, environmentally or financially. In the wallet or just for labor purposes. It's just a win, win, win situation. If you know, you don't have to use a product and instead you could choose a different one. [00:13:01] Craig Macmillan: Oh, this might be a related, very practical matter. So gloves, touching vines, no problem. I can have workers. I could have actually my VIT tech go down a row and then swipe the canopy. Where do I get the swabs because we're talking about something has to be a sterile protected swab, just like the COVID test swab. Where do I get those? [00:13:17] Sarah Lowder: Yeah so a lot of the times, or at least for a lot of this test that we did originally they were groups that we sent out, or like just kits that we would send out to the different cooperating practices. But essentially, it is literally the same tool. It's a polystyrene cotton swab tip and we normally will put them into these little half tubes that kind of keep their tip protected, and then you will stick it back into the plastic wrap that it came in, and then wrap it up so that it stays more protected. But it is, it is literally the same tool that you often will use for those COVID tests, which made it really hard to get that right at the beginning of the pandemic. [00:13:54] Craig Macmillan: Where, where can I get them? Where can I order them from? [00:13:56] Sarah Lowder: So I can send a link for the type of material that we're looking for. And then you can shop around for the same tip at a couple different locations and find the best price. [00:14:06] Craig Macmillan: That'd be perfect. We'll put a link to that in the show notes. So folks, you can go check that out and find the materials that you need. And then you can also shop around for the labs and see who's doing what. And again, if a lab is getting a lot of phone calls. That's going to encourage them to adopt this new technology. What is kind of the, the current state of the nation, if you will, in terms of fungicide resistance, what are the hot new topics, what's getting funded? What are people working on and where are they working on it? [00:14:33] Sarah Lowder: Fungicide resistance is certainly a hot topic that has a lot of different research happening in a lot of different areas. I could literally fill your entire podcast probably with talking about this topic. One thing that I'm very excited about is to hopefully see this Frame 2 iteration happen that is, being submitted for funding for this next funding cycle this year, but it is looking to hopefully also add in testing for downy mildew and betritis bunch rot, which are also two big diseases that can cause a lot of issues. Especially now that I'm over here in Georgia, I'm very excited to hopefully get to test much more of the downy mildew which can be quite the struggle in the vineyard. And we're getting much more information on a lot of the other modes of action that we're working with. I'm excited to see a lot of the information come out about some of the quinoxephins. Some of this information that we have started to work on and we haven't seen much field resistance to these products yet. But if we continue to use them, then they may still pop up. And so hopefully making sure that we can get ahead of the game for these other products. [00:15:37] Craig Macmillan: I'm very happy about that because I'm old enough to remember when the DMIs came out and it was like, Oh my god, it's a secret. It's the silver bullet. We're never gonna have to use sulfur again. Yay, and then it was literally within a couple of years they failed in some places. Now, the Fungicide Resistance Action Committee is publishing better information, more information, more accessible information every year about what's new about the different code groups. And they're also now putting a category in, or they have for a long time, but they have a category in there about the potential for resistance. So they'll say, yeah, there's known resistant populations for this. This one has got high potential, even if we haven't found it yet. And that's directing research in that direction. And I think that's really important that we stay ahead of these things. things, looking at where the potential is, as well as where there's known issues. So that's really fantastic. This is going on at what OSU, MSU, I think, Tim Miles in Michigan Walt Mahaffey in Oregon. I think Washington state. [00:16:35] Sarah Lowder: Mm hmm. Michelle Moyer is the viticulture specialist at Washington state university, who's been the project director for this project, led with Walt Mahaffey, who is the foliar plant pathologist at the USDA located in Corvallis. the horticultural crops units. And then a whole, a whole group of scientists from across the nation. There are people at UC Davis. There's people at Michigan State University. Even while I worked on this project in my role at Oregon State, I'm at with the USDA in Corvallis, Oregon. I'm now here at the University of Georgia working with Phil Brannon, who was the University of Georgia, Scientist working on this project and who actually was the person that convinced me to actually apply for the job that I currently now sit in. [00:17:20] Craig Macmillan: That's great. You know, we do still have a little bit of time left. This is a huge topic, but I found it interesting as I was doing research on you and looking at your publications. One of the things you've been involved in is research on collective action. and information transfer among growers related to disease management. And I am really fascinated by this topic. My background is in sociology. So my background is in people, talking to people, doing people stuff. And as we know, things, not just diseases, but also insects like mealybugs, etc. It's an area wide issue. And what you do or do not do on one farm affects what happens or doesn't happen on another farm. Can you tell us just a little bit about what that research was like, what you did? [00:17:57] Sarah Lowder: Absolutely. So that was really coming from how do we branch out from some of the fungicide resistance? Like how do we use a lot of this information that we are getting? One of the things that we were realizing is that some of the vineyards that we were working with, that we were getting some of these tests from, even organic vineyards who were not using any, they weren't using the QIs. They also weren't using any other synthetic product. We're still seeing sometimes very high rates. of the fungicide resistance to these different products. And what we really found was that a lot of the information that we were able to look at was really so much more useful on that larger scale when we were able to look at all of this data in the aggregate. And so one of the things, which I'm going to talk about my experience with the Oregon State, in the William Valley Vineyard growers especially, that they took all of this information, that they took the data, powdery mildew information that we are getting from their impaction spore traps. They were taking the fungicide resistance data that we were giving them, and they were sharing all this information with each other. And we're able to say, Hey, oh, I was using this product, you know, on my field this year and next year. And then I started to see a lot of issues. You didn't see it immediately, but you saw it pretty closely after I did, even though you followed the patterns elsewhere. And so they were able to take a lot of that information and aggregate it and share together and be able to use that much more quickly. I didn't talk too much about how much of the fungicide resistance that we were seeing. We definitely did see some differences in some of the states. that we were looking at, although it was generally pretty high rates of resistance to the QI products across the nation. That degree was less in a lot of the places in Oregon where a lot of this information started and where they shared a lot of this information from the get go. So one of the things that we wanted to look at was how does this information come across and then what do people do with that information? Absolutely. So when you get into the area wide management, when you, when you start talking about it, you're like, okay, while I manage my property, Unfortunately, a lot of these diseases aren't just staying on my property. If we could, if we could keep everyone isolated, then we wouldn't have to talk about a lot of this information management information management across all these different regions. What do we do once we have that knowledge and once we're sharing all of these spores back and forth even if we don't necessarily want to share that. But if we can also share some of the information on what we're all seeing, we can all manage it a little better. Even if it's just as simple as like, Oh, my sprayer was not calibrated correctly and I'm starting to see More powdery mildew in my vineyard blocks. If you communicate that to your neighbor, then they may be able to increase their spray intervals a little bit more in order to not lose their crop because they're going to have to deal with a much higher crop load. And they could be looking at the spore information. They could be looking at any of these monitoring effects that they're collecting. But if you can know that a source population nearby is going to be pushing more spores more readily, that's even before it's hitting your vineyard. So it's getting that information even a little faster than you would with some of the spore monitoring efforts, if that makes sense. [00:21:01] Craig Macmillan: That does make sense. And so, in terms of the collecting the information, this doesn't just have to be glove swabs or, you know, impaction traps. This simply can be people saying, hey, I'm seeing mildew pressure that's pretty intense, or I'm seeing it earlier, or whatever. Other people are saying, I'm not seeing it yet, but I know I'm downwind of you, for instance. Or, I know that my conditions are still very conducive, so I might be able to, like you said, increase my spray intervals, maybe check my calibration, check my coverage. It's a, it's a heads up. Basically from from one grower to another. How is this information shared? [00:21:34] Sarah Lowder: So there are lots of different ways that this information is shared One of the ways that we wanted to look is at just what did people find the most useful when they were Looking for different information on either new diseases or old diseases or all that kind of thing And some of the stuff that we were finding was that people really found their colleagues, just those person to person conversations that they were having to be the most useful information when managing any diseases. And while people certainly found, which I was happy to hear, that people found their viticulture specialists, their extension agents, to be extremely useful when helping to find this information, they really were still, the the most important part was their colleagues and their neighbors, the other growers in the area. And so you can look at different ways that people communicate, which we, one of the things that we did was look at a communication network. So just exactly how is that information flowing in the system? And there are certain people, which this may not necessarily be a huge surprise. But there are certain people that a lot of people go to, to learn more information about stuff, especially as someone that is looking at a region and be like, Ooh, I have one, this super great new tool, mate, I have something even cooler than the glove swabs. And now I need to tell. the growers about it. I need to see if we can actually use this in a commercial way that'll be viable. You can go to some of these more influential individuals within a region and then hopefully see that information spread a little bit more quickly than it might otherwise if you just kind of picked your people based on just where they were located or even just the closest ones you get your hands on. As it were. [00:23:09] Craig Macmillan: Gotta find the node. Gotta find the hub, [00:23:11] Sarah Lowder: find the node. . [00:23:14] Craig Macmillan: This is an interesting topic to me because one of the things that I think we've lost from a cultural standpoint is the coffee shop. People who are now retired growers have talked to me about how you're on your farm. You know, before sunup you get things running, you get going. People are doing what they're doing. Everything's fine. And then you go to the coffee shop about nine o'clock and everybody's there. And that's where the information would get transferred. And this goes back to the, you know, basic farming decades ago. We don't do that anymore. What we do is we're there at Sunup before Sunup, we get things running, we get done, then we're back in the truck and we're on the phone and we're going to the next ranch and we're not connecting with people. In that kind of social conversational way, the way that we used to. And so hopefully we can revive some of that either through meetings or through internet or through just simply networks, like you said, if there's a relationship, you can just simply call somebody and say, Hey, I'm starting to see this and hopefully we can build those social networks and see the collective benefits [00:24:12] Sarah Lowder: and find more ways to bring us together. [00:24:15] Craig Macmillan: Find more ways to bring us together in a world that seems to be forcing us apart, right? No. On this topic of managing diseases and monitoring diseases, is there one thing you'd recommend to our listeners around this? [00:24:27] Sarah Lowder: There are lots of different diseases in lots of different ways that a lot of things are managed and we talked a little bit about Collective action in the sense that a lot of times some diseases are more effectively managed on a much wider scale than an individual Farming unit so like you could do the best practices in your own vineyard but if you have Someone next door doing lawn best practices that could just mitigate all of the hard work that you just put into it. But it's hard to say anything on the large scale other than the fact that Talking more with those around you has a much wider benefit for disease management on all scales And just also grow better cultural practices, better more information on learning. I may be someone in the academia realm, but I always think that the more you can learn, the better. And your neighbors are in the same game as you are, especially in vineyards. I feel like the mantra of the rising tide lifts all boats is very much in effect. [00:25:24] Craig Macmillan: So we can learn from extension and from all that great stuff that's out there, but we can also learn from each other. Sarah, where can people find out more about you and your work? [00:25:31] Sarah Lowder: Yeah, so now that I'm over here at the University of Georgia, where I'm most easy to find is through the Viticulture Extension website. It's called the UGA Viticulture Blog. We post a lot of information relevant to those growers in the southeast, but we also have a blog posting that sometimes will go out via email if you would like to sign up for that, backlog of what we've posted. It's the easiest way to find me. I'm all around the University of Georgia system. [00:26:00] Craig Macmillan: Fantastic. Thank you so much. Our guest today was Sarah Lowdre. She's a Denture and Viticulture Specialist and Assistant Professor in the Department of Horticulture at the University of Georgia. Thanks for being here. This was a great conversation. [00:26:10] Sarah Lowder: Thank you, Greg. I had a great time. Nearly perfect transcription by Descript

Western Australian growers are urged to be on alert following the discovery of succinate dehydrogenase (SDHI, Group 7) resistance in spot form of net blotch (SFNB) in barley crops in the WA grainbelt. The resistance has been uncovered by the Centre for Crop and Disease Management (CCDM), which is a co-investment by the GRDC and Curtin University. In this podcast, CCDM researcher Dr Fran Lopez shares the details of this significant discovery and its consequences for the grains industry. Contact details: Dr Fran Lopez fran.lopezruiz@curtin.edu.au More information: https://grdc.com.au/news-and-media/news-and-media-releases/national/2020/september/sdhi-resistance-in-sfnb-of-barley-discovered-for-the-first-time-in-australia

SDHI Resistance in South Australia Did you hear about the fungicide resistance discovery in the Yorke Peninsula last year? Net form net blotch (NFNB) of barley was declared fungicide resistant to the SDHI fungicide fluxapyroxad. In this episode, CCDM fungicide resistance researcher Fran Lopez-Ruiz joins Megan Jones as they discuss the situation, talking about why it happened, how to manage it, and how this can be prevented in other Australian regions. First up they talk to Central Ag Solutions agronomist Sam Holmes who was the first to notice an issue with fungicide control and to alert SARDI. Sam talks about the factors that likely led to the resistance problem, including barley on barley rotation, the same variety grown throughout the region, and early sowing. Next they talk to Tara Garrard, SARDI cereals pathologist who advises on the best way to manage resistance in the region, by avoiding SDHI fungicides, avoiding the more susceptible varieties, and avoiding a barley on barley rotation. In the podcast Fran comments further on the fungicide resistance discovery, and why Australian growers are at higher risk of developing resistance, due to the larger paddock sizes, the limited modes of actions that are available and also the tighter rotations of the same variety. This podcast can be listened to on Apple Podcasts, Soundcloud, Stitcher, Spodify, Tune in, Pocketcasts and more, just search “Crop Disease Podcast”.

On the Teagasc Tillage Edge podcast this week, Michael Hennessy was joined by Deirdre Doyle, Technologist in Oak Park, Carlow who chatted about disease control in spring barley.   Deirdre explained that crops were very clean at the moment, partly aided by the recent dry weather, and by reasonably good varietal resistance to fungal diseases.  She pointed out that, despite the lack of disease at the moment, growers need to walk their crops before the application of the first fungicide. Deirdre was involved in research which re-looked at fungicide timings in spring barley.  She described the key timings are at mid to late tillering for the first timing and awns emerged for the final fungicide.  She also noted that a mix of key actives (triazole plus strob/SDHI) at a 50% rate will be sufficient in most cases.   As Chlorothalonil (Bravo) cannot be used after May 20th Deirdre explained there is no role for this fungicide in disease control this year either at the first or second fungicide timing in spring barley.  Growers can use Folpet at the final timing and the new chemistry Revysol will also be a useful addition.  For more episodes and information from the Tillage Edge podcast go to https://www.teagasc.ie/crops/crops/the-tillage-edge-podcast/ The Tillage Edge is a co-production with LastCastMedia.

With 330 samples sent in during the 2019 season from 173 farmers, net blotch resistance in the South of the WA grainbelt is now less of a mystery. In the next Crop Disease Podcast, CCDM Director Mark Gibberd joins Megan Jones as they discuss the results from the Barley Disease Cohort Project, where about 15 per cent of samples carried the mutation associated with fungicide resistance – CYP51A F489L – which were widespread throughout the grainbelt. First up they talk to Grass Patch farmer Dan Sanderson, who sent in a few barley leaves in September last year, and received a negative result for the mutation. Dan talks about his disease management strategies and how the result will help him manage disease going forward. Next they talk to Chris Robinson, a Farmanco Agronomist based in Kojonup, to hear his take on the results and what his clients will do differently this season, including the application of an SDHI fungicide to the program if needed. Mark then finishes with some key recommendations for managing net blotch resistance, and the next steps for the project, which aims to provide economical, regionally relevant advice for growers managing fungicide resistance. This podcast can be listened to on Apple Podcasts, Soundcloud, Stitcher, Spodify, Tune in, Pocketcasts and more, just search “Crop Disease Podcast”.

Dans notre Point Chaud de ce mercredi 15 avril, on recevait Sylvie Nony, membre de l'association Alerte Pesticides Haute Gironde et également membre de La Clé des Ondes. Les associations Alerte pesticides Haute Gironde (APHG) et Générations Futures Bordeaux (GF-Bx) ont écrit à la préfète de Gironde pour lui demander de ne pas valider la charte des riverains actuellement en consultation publique sur le site de la Chambre d'agriculture. Distances ridicules, pas d'abandon des CMR Cette charte qui doit rentrer en application en juillet ne répond pas aux exigences de protection des riverains, et c'est la raison pour laquelle les associations environnementales ne l'ont pas signée. La question ne se réduit pas à un débat sur des distances, de toutes façons ridicules. Elle est surtout celle de la nature des produits, et les associations n'ont eu de cesse de la soulever pour obtenir un véritable engagement des organisations agricoles à abandonner au moins les plus dangereux d'entre eux (les fameux CMR, cancérigènes mutagènes reprotoxiques, ou les perturbateurs endocriniens, ou encore les fongicides SDHi). Elles n'ont pas eu gain de cause, mais continueront de se battre pour cette cause essentielle. Quant à la consultation, elle interroge les citoyens girondins non pas sur la pertinence des mesures proposées dans la charte, qui, pour l'essentiel, relèvent de la simple application des règlements ou d'une déontologie élémentaire, mais (de façon tout à fait tendancieuse) sur leur satisfaction vis à vis des différents acteurs. Dans le questionnaire, rien ne permet au citoyen répondant de comprendre que cette charte, si elle est validée, permettra de réduire les distances minimum de pulvérisation prévues par la loi du 27 décembre 2019, déjà ridiculement faibles. 40 000 personnes directement concernées rien qu'en Haute Gironde Voilà pourquoi les deux associations ont décidé d'organiser une autre consultation que l'on trouve en ligne aussi, afin d'exposer de façon pédagogique et claire les enjeux de cette charte qui va conditionner les pratiques agricoles à venir et donc l'environnement de nombreux girondins. Pour ne prendre que l'exemple de la Haute Gironde, une étude de l'Observatoire régional de santé et de l'environnement a évalué que les 2 344 personnes habitent à 5 mètres des vignes, 7 600 à 10m, et 29 813 à 50m. Soit un total de presque 40 000 personnes sur une population de 86 000 habitants. C'est dire que la proportion de la population impactée est énorme ! Le mois d'avril est celui de la reprise des traitements, et à l'inquiétude habituelle s'ajoute celle de l'épidémie actuelle de Covid-19. Les associations demandent en outre à la préfète d'interdire au moins les produits nocifs ou irritants pour les voies respiratoires. C'est une question de santé publique majeure. On trouvera l'argumentaire des associations en ligne sur le site d'APHG](http://alertepesticideshautegironde.fr/consultation-bidon/) et sur le [site de GF Bx Photo de Une : Villeneuve-de-Blaye en vue aérienne (Google Map)

A topical monthly podcast on a number of issues brought to you by Syngenta. This month we cover: - Global grain consumption and currency fluctuations - Spring barley opportunities and advice - Launching a new SDHI fungicide into the market - Brassica agronomy - Agronomy update AMISTAR TOP® and SOLATENOL™ are Registered Trademarks of a Syngenta Group Company. AMISTAR TOP (MAPP 12761) contains azoxystrobin and difenoconazole. A formal application for the approval of SOLATENOL™ has been submitted to HSE-CRD for evaluation. All other brand names used are Trademarks of other manufacturers in which proprietary rights may exist. Use plant protection products safely. Always read the label and product information before use. For further product information including warning phrases and symbols refer to www.syngenta.co.uk.

Syngenta's third Cereals podcast is on new fungicide technology in wheat. We spoke to experts Bill Clark and Iain Hamilton, and 4th generation farmer Jeremy Margesson for their take on the role of new generation SDHI fungicide technology in winter wheat. Guest speakers:Bill Clark, NIAB TAG Commercial Technical DirectorIain Hamilton, SyngentaJeremy Margesson, Farmer