Podcasts about line1

The guys discuss why interest rates shouldn't get cut, AMPs new mega Qantas points bank account, how Club Med reinvented itself, Adam claims victory over powerful investors as Cettire's share price collapses, LVMH reign comes to an end and Adir's bookstore heartbreak.00:00 - Intro + LVMH15:17 - Instagram Ads20:50 - Bookstore Experiences25:36 - Curb Your Enthusiasm Story28:40 - Vaccines32:36 - Temple & Webster39:10 - Cettire46:00 - Club Med & The Eighth Power53:38 - LinkedIn Censorship?57:34 - Skip the Line1:05:25 - Qantas Points1:21:10 - LE's New Loyalty Program 1:28:00 - Interest RatesThis week's sponsors:Netwealth: ⁠www.netwealth.com.au⁠Telstra: ⁠www.telstra.com.au/tbtc⁠Vanta: ⁠www.vanta.com/contrarians⁠ Thanks for listening!Join us on LinkedIn: https://www.linkedin.com/company/the-contrarians-with-adam-and-adir-podcastSubscribe on YouTube for all our video content: https://https://www.youtube.com/@ContrariansPodcastFollow us on Instagram: https://www.instagram.com/contrarianspodFollow us on TikTok: https://www.tiktok.com/@contrarianspod

Most (if not all) previous Ambientblog mixes always had some dark moments in them, they are never 'just' relaxing. You can only feel release if you also felt tension. This time, I wanted to avoid the darkness and create a 'lighter mood mix'. Not of the 'Ibiza Chillout Lounge' kind, but one that can be played in the background and is still exciting enough to listen to. I hope I succeeded, but of course, you're the one to decide about that!With two hours, Cloudwatching is longer than usual. Only a few tracks are included in their full length, but most are heavily edited or even just short fragments. Also, they are often overlayed, so you'll hear the music in a different context. The extensive playlist below may help you track down the original in their full-length versions. I really hope you'll enjoy watching these -imaginary- clouds! Tracklisting Start Length Artist - TitleAlbum Title, Year, Label 00:00 3:58 Francisco López - Untitled #373Untitled, 2019, Esc.Rec00:17 4:09 Lawrence English - Pre-Approach - CitizenApproach, 2022, Room4001:47 6:04 William Basinski & Janek Schaefer - … On Reflection (Four)… On Reflection, 2022, Temporary Residence Limited06:15 4:02 Arve Henriksen, Eivind Aarset, Jan Bang - The Swans Bend Their Necks Backward To See GodThe Height Of The Reeds, 2018, Rune Grammofon09:22 2:59 Masayoshi Fujita - HarpBook Of Life, 2018, Erased Tapes11:12 4:07 Mombi Yuleman - Mental TelepathyHours Lost, 2022, ZeroK13:14 2:31 Asmus Tietchens - Paralelle Ebene 1Parallelen, 2022, Line14:26 2:41 Abul Mogard - Like WaterStrega Music, 2022, Make Noise Music16:14 2:48 Keda - La Lune De CoréeHwal, 2015, Bruit Clair18:31 4:27 Tapani Rinne & Teho Majamäki - ReflectionOn the Border, 2022, Signature Dark22:34 4:01 Radboud Mens - ConvolutionContinuous, 2022, ERS26:02 3:03 Alex Haas & Michel Banabila - The Woods (ft. Bill Laswell)Singles (2020-2022), 2022, Tapu Records27:58 2:01 Imperial Valley - Fields North Of Kane Spring, Oct 8, 1938Imperial valley III, 2022, Folded Rime29:03 3:47 James Murray - ClearingsCareful Now, 2022, Home Normal31:41 2:02 Raum - DaughterDaughter, 2022, Yellow Electric32:04 3:05 Tierro Cosmico - Eres NocturnaFigments Of Wonder, 2022, Neotantra34:29 2:34 Emmanuel Holterbach - Rivage (Shore)Le Rêve, L'Ombre Et La Vision, 2022, Canti Magnetici35:09 3:51 Holland Patent Public Library - A Road I've Passed But Never TakenSongs To Fall Asleep At The Wheel To, 2022, Dear Life Records38:34 2:11 Thomas Ragsdale & Richard Arnold - The ViewChasing Waves, 2022, Frosti39:24 3:50 David Cordero & Kenji Kihara - GrazalemaKomorebi, 2022, Home Normal42:27 2:14 Abul Mogard - The Rain Has GoneFor Ukraine Vol. 3, 2022, Headphone Commute44:06 3:43 Robyn Miller - GateroomRiven - The Soundtrack, 1998, Virgin47:34 2:12 From The Mouth Of The Sun - Paint & StoriesA Broken House, 2021, Dronarivm49:01 4:23 Olga Wojciechowska - Recreating WorldsFor Ukraine Vol. 3, 2022, Headphone Commute52:50 3:13 Biosphere - Aura In The Kitchen With The CandlesticksDeparted Glories, 2016, Smalltown Supersound55:35 1:46 Jolanda Moletta - Spell II: Daydream And NightbloomNine Spells, 2022, Ambientologist56:32 1:48 Budhaditya Chattopadhay - DhvaniAnthology Of Exploratory Music From India, 2021, Unexplained Sounds Group57:01 6:29 Cell - GeigerOnwards System, 2022, Ultimae Records1:02:46 1:33 Clarice Jensen - FearEsthesis, 2022, FatCat Records1:03:19 4:06 Erik Wøllo - Peace BellsSojourns, 2022, Projekt1:06:30 4:07 Tarotplane - AuximenesAeonium, 2022, Constellation Tatsu1:09:30 2:39 Norman W. Long - Return & RecoveryReturn & Recovery, 2022, Line1:10:51 3:08 Marine Eyes & Antarctic Wastelands - EnshroudedSustain Series, Vol. 3, 2022, Ambientologist1:13:54 1:34 Ryoji Ikeda - Data.Tron (2007-14)Music For Installations Vol. 1, 2021, Codex Edition1:14:37 2:07 Masayoshi Fujita - PonsBird Ambience, 2021, Erased Tapes1:16:39 3:32 Tetsuroh Konishi - Kevaan AaniaScenes, 2021, Off-Recordlabel1:19:38 3:30 Madeleine Cocolas - PresenceSpectral, 2022, Room401:22:17 1:49 Pinkcourtesyphone - Comfortable PredictabilityAll Intensive Purposes, 2022, Room401:23:51 4:10 Clarice Jensen - LoveEsthesis, 2022, FatCat Records1:26:16 3:47 Radboud Mens - ModularMovement, 2022, ERS1:29:16 2:08 Arve Henriksen - The Farmers Of Sonic AlchemyMurimorphosis, 2021, Arve Music1:31:18 2:10 Daigo Hanada - KageSatori, 2022, Moderna Records1:33:04 3:21 Ivan Cebrian - Snow In SilenceInside/Outside, 2022, Oigovisiones1:35:53 3:34 Robert Rich & Luca Formentini - First DayFor Sundays When It Rains, 2022, Soundscape Productions1:39:20 1:48 Sofie Birch & Antonina Nowacka - OutroLanguoria, 2022, Unsound / Mondoj1:41:03 1:50 Sven Laux & Fione - StayTomorrow Everything Could Be Different, 2022, Whitelabrecs1:42:07 4:16 Loscil - Dub For CascadiaEndless Falls, 2010, Kranky1:45:50 2:22 Powlos & Holt - Our Shimmering BreathSustain Series, Vol. 3, 2022, Ambientologis1:47:48 3:33 Anne Chris Bakker - Johan (In Memoriam)A Sketch In Leaving, 2022, Whitelabrecs1:50:45 2:34 Glåsbird - SirsaHimalaya, 2022, Whitelabrecs1:53:15 1:04 Trio Ramberget - D Minor Postlude24 Ways Vol. 1, 2022, Piano And Coffee Records1:54:11 2:08 Galya Bisengalieva - The CrashHold Your Breath: The Ice Dive OST, 2022, One Little Independent Records1:55:48 4:11 Madeleine Cocolas - EnfoldSpectral, 2022, Room40

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements LINE1 retrotransposons between species. University of Southern California professor Joseph Hacia discusses studies profiling phytanic acid levels in red blood cells obtained from humans and captive non-human primates all on low phytanic acid diets. Emory University professor James Rilling discusses the difference of arcuate fasciculus between human and non-human primate brains and how the specialization of speech has helped humans evolve. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37527]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Identifying cellular and molecular differences between human and non-human primates is essential to the basic understanding of the evolution and diversity of our own species. While preserved tissues are the main source of comparative studies between humans, chimpanzees and bonboos, the samples do not accurately represent the traits of live cell behavior and cannot be genetically manipulated. UC San Diego professor Carol Marchetto discusses how a comparative gene expression analysis of human and non-human primates revealed differences in the regulation of a class of transposable elements (Long Interspersed Nuclear Element 1 or LINE1 retrotransposons) between species. Series: "CARTA - Center for Academic Research and Training in Anthropogeny" [Humanities] [Science] [Show ID: 37377]

Brian Orlando and Om Patel go back to basics in part one of our walkthrough of each of the Scrum Events! In this episode, we talk about the Daily Scrum and the Sprint Retrospective. We discuss our experiences and the different ways to approach and facilitate each event.0:00 Daily Scrum - 3 Questions13:36 Daily Scrum - Walk the Board19:37 Why Do the Daily Scrum at All?28:47 Daily Scrum for a New Team33:44 Retrospectives42:45 On-Demand Retro May Have Helped51:00 Faux Statuses56:06 Stopping the Line1:03:43 Retro Topic WrapAlso available on Youtube: https://www.youtube.com/watch?v=fHxmvad9W50

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.22.214262v1?rss=1 Authors: Shiraishi, Y., Koya, J., Chiba, K., Saito, Y., Okada, A., Kataoka, K. Abstract: We introduce our novel software, nanomonsv, for detecting somatic structural variations (SVs) using tumor and matched control long-read sequencing data with a single-base resolution. Using paired long-read sequencing data from three cancer cell-lines and their matched lymphoblastoid lines, we demonstrate that our approach can identify not only somatic SVs that can be captured with short-read technologies but also novel ones especially those whose breakpoints are located in repeat regions. In addition, we have developed a workflow for classifying mobile element insertions while elucidating their in-depth properties such as 5' truncations, internal inversion as well as source sites in the case of LINE1 transductions. Finally, we identify complex SVs probably caused by replication mechanisms or telomere crisis by examining the co-occurrence of multiple somatic SVs in common supporting reads. In summary, our approaches applied to cancer long-read sequencing data can reveal various features of somatic SVs and will lead to further understanding of mutational processes and functional consequences of somatic SVs. Copy rights belong to original authors. Visit the link for more info

Lonya and Jeremy take the TWiV team beTWIXt primate immunodeficiency virus proteins Vpx and Vpr and how they counteract transcriptional repression of proviruses by the HUSH complex. Hosts: Vincent Racaniello, Dickson Despommier, Rich Condit, Kathy Spindler, and Brianne Barker Guests: Leonid Yorkovetskiy and Jeremy Luban Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Pan American Society for Clinical Virology San Diego and Houston Regional Meetings Vpr and Vpx counter HUSH (Nat Micro) Vpr and Vpx counter HUSH (bioRxiv version) Schematic of HUSH complex (Lehner lab) Olive colobus monkey sound credit Image credit: William (Ted) Diehl Letters read on TWiV 516 Timestamps by Jolene. Thanks! Weekly Science Picks Brianne - The Chimp and the River by David Quammen Rich- James Cook’s First Voyage Journal (20 sec Google Earth summary) Kathy- High resolution Antarctica map NYTimes 46MB Maps (more info and 43 tb maps) Dickson- Jellyfish Jeremy - Pictorial Guide to Living Primates; All The World's PrimatesLonya- Chougui et al. HIV-2/SIV Vpx counters HUSH Vincent - Research!America Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

We continue our hangout with Nate & Liv and as the whiskey settles in, they tell us the story of their most recent trip to Japan.  They discuss some sexy BDSM-style hotel rooms (see the image for Episode 24A) and Nate revisits his old sex dojo for some solo fun!  We talk about exploring solo play and the conversations that occur behind close doors leading up to that point. Mentioned in this episode: Line1_n_Line2 - Nate & Liv

We were invited to a onesie-themed pajama party where we were going to play board games/card games and maybe watch some Netflix and chill but we brought a game of our own that somehow led to a wild and unpredictable Saturday night where "chill" turned into DP sex with a lesbian couple.  We also have the first in our series of listener "car ride home" stories at the end of the podcast and linked here below.  A couple from Miami shares their car ride home story here.  She was so wet... Mentioned in this podcast Line1_n_Line2 @Twitter

感谢热心听友“大声Helen”帮忙听写本篇文稿!Ryan (R): A Beijing couple has decided to break tradition and used the subway for their wedding party’s transportation. Crazy or cool? What’s going on? Bob (B): Well, I mean, Niu Hongling and I have beenfighting over this story since... but is actually does calm down to whether this is a wedding for human beings or for cats.R: How does it come down to that Bob?Niu Honglin (N): I have nothing to do with it.B: Because I think it would totally be different if this was cats? Anyway it was a story. OK. So hiring a limousine.. It’s a headache. The whole wedding thing is a headache, isn’t it? For couples who want to get married. So much they have to think about getting from A to B, moving the whole wedding party around that kind of things. It’s a huge expense. And in these days, when we’ve got to think about the environment and you know the kind of things that... What would we rather spend our money on? Do we rather spend it on luxury limousines? Or do we want to maybe spend it on things for the new house or something like that? Then you think it yourself, I KNOW! Let’s have the wedding in the subway. Good idea or bad idea, I don’t know.N: Well, I have to justify for this specific couple. Well, in their case, they didn’t actually have the wedding in the subway. They are just using subway as a transportation choice. So they decided to go from home to the hotel using the subway, but wearing traditional Chinese wedding customs. And they walked into the subway station with their bridesmaid and best-man. And actually it’s very sweet, cuz the bridegroom checked the regulation beforehand to make sure that they didn’t violate any rules. And they set out at 8am Saturday to avoid disturbing traffic peak hours, keeping the ride low key without any special music or arrangements during the trip. So I believe it’s a cute and sweet idea and they didn’t disturb anyone. They kind of spread their joy and they have made their contribution to promoting a green idea of living.R: I want to talk about how Bob wants to spread joy with the cat in the subway as he was referencing... I do believe? I don’t know. But you know, looking at this, I mean I don’t think we can comfortably say nobody was a little annoyed. Someone is gonna have to take the cab, not the cab, the subway. Because the cabs are maybe a little more expensive if you are cab hailing, maybe taking the subway on a Saturday. So in which case, if you’re taking the subway in the morning, for whatever reason, on a Saturday someone is, at 8am, then do you think this would annoy you? I seem to be under the impression anyone waking up at 8am on a Saturday is grouchy. Right, I would be, I would be grouchy and then I see these people having a great time, getting married... And I am single, I’m doing something at 8am in the morning. B: Oh, he’s bitter.R: And I’m like rub it in my face, could you just take your taxi with your tinted windows, So I don’t even have to see it. Bob.B: Just calm down. R: You’re gonna on the side with me Bob?B: No, not in the slightest. So do you think it’s a good idea or not a good idea before I go onto cats?R: Oh, God.N: I think it’s a good idea for them. Because they are not taking a limo. They are taking the subway. And I feel like they are not really disturbing anyone. And also a lot of netizens have responded, saying that renting a limo is not that important and their way of doing this is environmental-friendly. And it’s possibly faster concerning Beijing’s traffic conditions. So I’m agree with them doing it.B: Do you know, I think it depends on which subway line that this was going to happen on. If it’s subway Line1, then I reckon that there’s a lot of grumpy people. R: Yeah, me.B: You, for example. Sitting there, being grumpy and saying go get married somewhere else.R: Bring cats.B: But on some lines, I reckoned, on Line6. This would be fine. Everything is a bit more chilled on Line6.R: I don’t take Line6 often, so I can’t say. But definitely on Line1...B: And we’re talking about Beijing Subway here?R: Yes, definitely on Line1. I would be a little bit grumpy. Niu Honglin?N: Yeah, for me, as long as you are happily married, you will spend your day with your loved ones, friends and relatives. You can do whatever you want as long as you are not bothering anyone, you have my bless.B: But use cats.R: How did cats ever get into this? You got to explain yourself here.B: Cuz everybody loves cats.R: That’s not true. Some people love dogs.B: Or dogs. Okay, I think more people like cats. But if you’re going to do something which is really sort of... When it comes to society like getting in people’s way. Then you just would think, okay I gotta do something to make people feel happy, in which case, take a cat with you. And then, everybody would be happy. No, you didn’t get away with more. I have done it myself.R: See, this is where it starts. This is what I’m worried about guys. And I want your opinions. I starts with weddings. But then it ends up being with Bob with cats on the subway. And who knows how many cats Bob will bring with him on the subway?B: I’m not a crazy cat-man. I’m not gonna bring you like 10 cats or something like that.N: Well, let me bring the subject a little more to human wedding instead of cat wedding. For those of you who really want some creative ideas. You can also take some water ways, you can also take a bike and also a horse might be a nice choice. For those of you who want to have some adventure, you can get married on an airlift. And also you can rent a party bus to transport all your guests, relatives and friends. So whatever you want to do, just be happy and creative.R: Guys, gotta know, quick closing statements, cute or annoying? What do you think Bob?B: I think, annoying on Line1 and probably quite entertaining on Line5.R: Oh my God.N: Cute and happy and full of pink bubbles.B: And cats.R: Or maybe cats. That could definitely just be you, Bob, on this one.

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The Twivsters discuss how reverse transcriptase encoded in the human genome might produce DNA copies of RNA viruses in infected cells. Links for this episode Should variola virus be destroyed? (poll at virology blog) DNA complementary to non-retroviral RNA viruses (Sci Rep) Integration of arenavirus DNA into cell genome (virology blog) Infectious respiratory syncytial DNA (PNAS) Tysabri and PML JC and PML (J Imm Res) Igor Koralnik laboratory Jean-Luc Doumont (Kathy's pick, TWiV 268) Bullet points kill (TED) Letters read on TWiV 288 Weekly Science Picks Rich - LuvalampsAlan - ExperimentVincent - American Society for Virology on FacebookKathy - LEGO female scientistsDickson - The Oldest Living Things in the World by Rachel Sussman Listener Pick of the Week Basel - A Treatise on the small-pox and measles by Abu-Bakr Al-Razi Send your virology questions and comments (email or mp3 file) to twiv@twiv.tv

Stochastic, environmentally and/or genetically induced disturbances in the genome-wide epigenetic reprogramming processes during male germ-cell development may contribute to male infertility. To test this hypothesis, we have studied the methylation levels of 2 paternally (H19 and GTL2) and 5 maternally methylated (LIT1, MEST, NESPAS, PEG3, and SNRPN) imprinted genes, as well as of ALU and LINE1 repetitive elements in 141 sperm samples, which were used for assisted reproductive technologies (ART), including 106 couples with strictly male-factor or combined male and female infertility and 28 couples with strictly female-factor infertility. Aberrant methylation imprints showed a significant association with abnormal semen parameters, but did not seem to influence ART outcome. Repeat methylation also differed significantly between sperm samples from infertile and presumably fertile males. However, in contrast to imprinted genes, ALU methylation had a significant impact on pregnancy and live-birth rate in couples with male-factor or combined infertility. ALU methylation was significantly high-er in sperm samples leading to pregnancy and live-birth than in those that did not. Sperm samples leading to abortions showed significantly lower ALU methylation levels than those leading to the birth of a baby. Copyright (C) 2011 S. Karger AG, Basel