Podcasts about ten simple rules

Guests Eriol Fox | Allen “Gunner” Gunn | Leslie Hawthorn | Abby Cabunoc Mayes Panelist Richard Littauer Show Notes In this special episode of Sustain, Richard has a discussion with guests and fellow hosts Eriol Fox, Abby Cabunoc Mayes, Leslie Hawthorne, and Gunner, about the recent virtual Sustain event discussing the current state and future of sustaining open source software. The conversation covers a broad range of topics, from the impact of AI on open source and the complexity of corporate funding, to the importance of succession planning and the need for a continued focus on equity and inclusion. The group also express gratitude to the community and highlight the necessity of creating safe spaces for deep and meaningful discussions about the human aspects of open source. Future events and potential topics are also teased. Hit the download button now! [00:01:45] Gunner shares an event summary starting on the evolution of SustainOSS, and talks about the topics ranging from usage metrics, donor programs, geopolitical barriers in FOSS, and details the working sessions. [00:03:34] Everyone shares their personal takeaway from the event. [00:09:57] We hear about the conversations that were missed at the event and what everyone would like to talk about in the future about sustaining open source. [00:17:56] Gunner briefs us on the next event with a possibility of another forum focused entirely on AI, and Richard proposes exploring digital sovereignty and how it intersects with open source principles. [00:19:39] We end with final thoughts from everyone: Gunner expresses gratitude for the community, Abby is grateful for the space and conversations, Leslie gives a shoutout to UN Open Source Week for fostering global cooperation, Eriol praises Jonah Duckles for putting out “Ten Simple Rules for Academic Open Source Collaborations with Industry,” and Richard encourages listeners to keep contributing and engaging with the Sustain community. Links SustainOSS (https://sustainoss.org/) podcast@sustainoss.org (mailto:podcast@sustainoss.org) richard@sustainoss.org (mailto:richard@sustainoss.org) SustainOSS Discourse (https://discourse.sustainoss.org/) SustainOSS Mastodon (https://mastodon.social/tags/sustainoss) SustainOSS Bluesky (https://bsky.app/profile/sustainoss.bsky.social) SustainOSS LinkedIn (https://www.linkedin.com/company/sustainoss/) Open Collective-SustainOSS (Contribute) (https://opencollective.com/sustainoss) Richard Littauer Socials (https://www.burntfen.com/2023-05-30/socials) Eriol Fox Website (https://erioldoesdesign.github.io/) Allen “Gunner” Gunn LinkedIn (https://www.linkedin.com/in/allen-gunn-acab/) Leslie Hawthorn LinkedIn (https://www.linkedin.com/in/lesliehawthorn/) Abby Cabunoc Mayes Website (https://abbycabs.github.io/) Monki Gras 2025 (https://monkigras.com/) UN Open Source Week 2025 (https://opencommons.org/UN_Open_Source_Week_2025) Ten Simple Rules for Academic Open Source Collaborations with Industry by Jonah Duckles, Dan Sholler, Beth Duckles (https://orgmycology.com/10-simple-rules-for-academic-industry-collab/) Credits Produced by Richard Littauer (https://www.burntfen.com/) Edited by Paul M. Bahr at Peachtree Sound (https://www.peachtreesound.com/) Show notes by DeAnn Bahr Peachtree Sound (https://www.peachtreesound.com/)

We've mentioned in the past that the code we write isn't maintaining heartbeats or being used in life-critical settings, but what if your code is? NASA happens to be a company who has code that is life and mission critical and it's very possible it won't even be accessible once it leaves earth. In this […]

We've mentioned in the past that the code we write isn't maintaining heartbeats or being used in life-critical settings, but what if your code is? NASA happens to be a company who has code that is life and mission critical and it's very possible it won't even be accessible once it leaves earth. In this […]

If we want to change our politics, let's start by chucking out the media's obfuscating narratives and pinpointing the incentives that drive it. Bradley lays out his Ten Rules that Demystify Politics. Plus, why prosecutors haven't laid a glove on Trump, what Elon ought to do with Twitter and the amazing debut of Stupid Business Pitches.

dessanをゲストに迎え、CRISPRの仕組みを利用した様々な技術や遺伝子回路、これからの発展について話しました。Show notes The Nobel Prize in Chemistry 2020…The Nobel Prize in Chemistry 2020 was awarded jointly to Emmanuelle Charpentier and Jennifer A. Doudna “for the development of a method for genome editing.” Scientifc Background on the Nobel Prize in Chemistry 2020 A TOOL FOR GENOME EDITING…ノーベル財団による詳細なCRISPR研究のレビュー、そしてなぜDoudnaとCharpentierの二人が受賞に値するのかについて説明している。 76. The Chimeric RNA, Researchat.fm…ゲノム編集についてdessanをゲストに迎えて話しました。 A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science 2012…CharpentierとDoudnaによるノーベル賞につながる論文の一つ。CRISPR–Cas9システムがこの論文によってその大枠が明らかにされた。 Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 2013…Feng Zhang labによるヒト細胞におけるゲノム編集技術の報告。 RNA-Guided Human Genome Engineering via Cas9. Science 2012…George Church labによるヒト細胞におけるゲノム編集技術の報告も同時に掲載された。 First rounders: Feng Zhang (Podcast)…Feng Zhangが出演したNatute Biotechnologyのポッドキャスト。おすすめです。 26. Cool tech googlability, Researchat.fm…RNAを標的にできるCas13bについては、エピソード26で紹介しました。 Cas14 (crisp_bio)…“Cas14は、PAMに依存しないssDNA切断活性に加えて、PAMに依存するdsDNA切断活性も帯びている” CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature 2019…CasX Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration. Nature 2019…トランスポゾン型のCasシステムの報告。 RNA-programmed genome editing in human cells. eLife 2013…Doudna labによるヒト細胞におけるゲノム編集技術の報告。FengやChurchらよりも少しだけ遅かった。 Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9. Nature Communications 2014 Ep52. Split into a row Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 2013…Double nicking (2つのgRNAとCas9 nickase)によるより正確なゲノム編集方法が示された。 Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 2015…Cas9を用いた転写の活性化手法。 Live visualization of chromatin dynamics with fluorescent TALEs. Nature Structural & Molecular Biology 2013 … TALENを用いた染色体の特定領域のイメージング方法 Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell 2013…dCas9-EGFPによる生細胞のイメージング技術。SpCas9の場合は、D10AとH840Aの２つの変異を入れることで、DNAに結合するが切断しないdead Cas9 (dCas9)として利用することができる。 Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. Nature Communications 2017…ガイドRNAにMS2 loopをたくさんつなげることで (14個!)、明るい輝点を得ることができる。 CRISPR-mediated live imaging of genome editing and transcription. Science 2019…こちらは蛍光標識したガイドRNAを利用した生細胞イメージング方法。 A protein tagging system for signal amplification in gene expression and fluorescence imaging. Cell 2014…Sun tagとCas9を用いたイメージング方法。 Split Green Fluorescent Proteins: Scope, Limitations, and Outlook…Split GFP Programmable RNA tracking in Live Cells with CRISPR/Cas9. Cell 2016…PAMmerによるSpCas9のmRNAイメージング CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization. Cell 2018 … CRISPR-GO：CRISPR技術、核内でのゲノム空間構造、ポッドキャスト内ではゲノム同士を寄せるという説明をしていましたが、今調べてみると特定のゲノム領域と核膜やカハール体への再配置ということでした。 Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping. Nature Communications 2017 … こちらがCRISPRの仕組みを用いることで人工的に染色体内部にループを作成した論文。 Transcriptional repression mediated by repositioning of genes to the nuclear lamina. Nature 2008 … LacO-LacIの仕組みを用いることでゲノムの特定領域にLacO arrayを差し込み、核膜に局在させたLacIに結合させることである遺伝子領域を核膜側に誘導しようとした論文。最初にこの論文を読んだ時はそのアイデアにたまげました。 9. One-shot beautiful experiment (Researchat.fm)…人工的なDNA領域へ細胞内の情報（細胞系譜）を書き込む技術についてエピソード9で話しました。 CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria. Nature 2017…George Churchらは、Cas1-Cas2システムによって馬の動画をバクテリアゲノム書き込み、それを読み出すことに成功した。 Multiplex recording of cellular events over time on CRISPR biological tape. Science 2017…コピー数の異なる2つのプラスミドをCas1-Cas2で取り込ませて、細胞内で人工的な時計のような仕組みを実現した。 Single-Nucleotide-Resolution Computing and Memory in Living Cells. Molecular Cell 2019…Tim Liu Labによる複雑な遺伝子回路の実現。DOMINOについては、プロモーター配列を標的にしているのではなくオペレーター配列でした。 Rewritable multi-event analog recording in bacterial and mammalian cells. Science 2018…David Liu labから報告されたガイドRNAによって連鎖する遺伝子回路（カスケード）の実現。 Terminal Deoxynucleotidyl Transferase, TdT…テンプレートに依存しないDNA合成を可能にする酵素。 Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nature Biotechnology 2012…GFP Timer Permanent genetic memory with >1-byte capacity. Nature Methods 2014 Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science 2016…自分で自分のガイドRNAを編集することで、理論的には無限に情報を書き込む方法が提案されたが、領域が壊れてしまう問題もある。 Ten Simple Rules to Win a Nobel Prize. ヘンリー・ブラッグ (Wikipedia) iPS細胞 (Wikipedia) 国境なき医師団 Human Genome Project Xiaowei Zhuang Expansion microscopy (Wikipedia) Renato Dulbecco (Wikipedia) Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nature Biotechnology 2019…LEAPER crisp_bio … 世界広しといえでも、これだけCRISPRの最新情報がまとまっているサイトはCRISP_BIOさんの他に世の中には存在しません。日本語でCRISPRの最先端情報を追える喜び。CRISP_BIOさん、いつもありがとうございます。 Editorial notes 1分でわかるとか無理なのですが、一方で言葉を尽くせばわかる可能性についても同時に信じておりますので…(soh) 思い出しながらどんどん話しているので、後から聞き返すと細部が間違っていたりしています。気になった方はshow notesをご参照ください。(dessan) いい感じのグルーヴがみられてよかったです。ポッドキャストやってきてよかったです。(tadasu) 最初に喋らんと出番が無くなる！と思ってこれまでの流れをまとめてみたんですが細かく色々ミスってました…(coela)

Racial and ethnic diversity within the Science, Technology, Engineering and Math (STEM) fields remains low, and many scientists and researchers are seeking solutions to help address racism in their workplaces. To help, assistant professor and environmental scientist in the Department of Environmental Science and Studies for DePaul’s College of Science and Health, Dr. Bala Chaudhary, collaborated with another researcher of color to create the “Ten Simple Rules for Building an Antiracist Lab.”

NEB社の巨大な製品カタログの楽しみ方、多様な制限酵素の世界とその巧妙な仕組みについて話しました。 第123回　ポッドキャストで語るサイエンスとその魅力(実験医学) NEB…日本語のNEBウェブサイト。NEBはNew England BioLabsの略でした(BioScienceではありませんでした。)大変申し訳ございませんでした。 NEB Literature Portal…カタログのまとめページ。 NEB Catalog & Technical Reference…これがNEBが誇る400ページ超のカタログ！ Eric Lander (Wikipedia) DNA Structure and Classic experiments (Youtube)…Eric Landerの超おすすめなMITの授業動画。見よう！ 制限酵素の基礎知識 (ThermoFisher)…とてもわかり易いのでぜひ。 ファージ (Wikipedia) DNAトポイソメラーゼ (Wikipedia) ニック EcoRI…このエピソードで例として紹介しました。GAATTCという配列を認識して切断する。 回文配列(パリンドローム) DNA ligase…DNA断片同士を連結させることができる。 Gibson Assembly…配列の相補性を利用して２つ以上のDNA断片を試験管内で連結する方法。Dan Gibson博士が作ったのでみながそう呼ぶようになった。彼が自分でつけたわけではない。 DNAメチル化 Talen…ゲノム編集法のうちの一つ。 メタゲノム解析 (Online ISSN : 1881-6681) CasX enzymes comprise a distinct family of RNA-guided genome editors…地下水にすむバクテリアから単離されたCas-X オペロン (Wikipedia) IRES (Wikipedia)…Internal Ribosomal Entry Siteの略 REBASE…制限酵素のデーターベース。 IUPAC Codes - Bioinformatics.org…DNAの縮重塩基はこのように表現されます。 正規表現 Homing endonuclease I-SceI…ホーミングエンドヌクレアーゼの一種でとても長い認識配列を持つ。 I-SceI mouse model…ゲノム中にI−SceIサイトをもったマウスに対してI-SceIを発現させると二本鎖切断応答を誘導できる。この研究 (Nat. Comm 2016)など。 Miné-Hattab and Rothstein, Nat Cell Biol. (2012)…I-SceIを誘導して染色体動態の変化を観察した名論文 MmeI EcoP15I CAGE法…FANTOMの基幹技術。MmeIを用いたトリックが秀逸。 DpnI (NEB)…メチル化されたDNAのみを切断する DamID (DNA adenine methyltransferase identification) RNA splint ligase (NEB)…“SplintR LigaseはRNA splint上のDNAをライゲーションする” NEB podcast Science podcast Nature podcast iBiology デヴィッド・リンチ Ten Simple Rules to Win a Nobel Prize マシュー・メセルソン ショ糖密度勾配法…初期分子生物学の重要技術。正直やったことがないです… DNAの半保存的複製 ep7. In the golden age of molecular biology…メセルソン先生とmRNA発見の話についても触れています。 Twitter @researchat_fm Researchat.fm Editorial notes 普段あまり考えずに使っていた制限酵素について、もともとの機能や精製方法を知りとても面白かった。みんなカタログを読もう！(soh) DNA修復はすでにノーベル賞が来ていますね。メセルソン先生の偉業の数々もいつかしっかりまとめて話したい。(tadasu)

There's nothing simple about solving racial inequity in science, but here is one place to start.

Our guest for this episode is Dr. Holly Bik. Holly obtained her PhD in Molecular Phylogenetics at the University of Southampton, working with John Lambshead at the Natural History Museum of London in conjunction with the UK National Oceanography Center. She completed postdoctoral appointments with Dr. Kelley Thomas at the University of New Hampshire and Dr. Jonathan Eisen at UC Davis before starting her faculty position. In addition to her research, Holly is invested in science communication. She serves as an associate editor for the popular marine blog Deep-Sea News and maintains an active presence on Twitter (@hollybik). Holly has co-authored a number of peer-reviewed articles on the use of social media and online tools in academia, including “An Introduction to Social Media for Scientists” in PLoS Biology and “Ten Simple Rules for Effective Online Outreach” in PLoS Computational Biology. In this episode, we discuss the first paper to come out of Holly’s lab at UC Riverside , entitled “Nematode-associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats” (Schuelke et al., 2018). Holly elaborates on the unexpected results from this paper and talks about the many challenges associated with collecting and analyzing marine sediments. In addition to the technical aspects of this paper, we also talk about time management and how Holly set aside time to write a draft in one week. She tells us about her 6-month-long personal work/life balance experiment in time-tracking and shares what she learned from this experience. We discuss the concept of Deep Work and why she continues to fill out weekly review worksheets to help manage stress and productivity. At the time of this recording, Holly was in the process of moving her lab from UC Riverside to The University of Georgia where she is currently an Assistant Professor in the Department of Marine Sciences. We talk about the process of moving and the factors Holly considered when making this important career decision. Holly explains that it's important for early career researchers to understand how long things take, and also be okay with the fact that some things are just going to take way longer than you expect. SHOW NOTES: Paper: Schuelke, T., Pereira, T. J., Hardy, S. M., & Bik, H. M. (2018). Nematode‐associated microbial taxa do not correlate with host phylogeny, geographic region or feeding morphology in marine sediment habitats. Molecular Ecology, 27(8), 1930-1951. A few of Holly’s Twitter threads: Data-driven time management https://twitter.com/hollybik/status/1133750210331496450 Concept of ‘deep work’ https://twitter.com/hollybik/status/1133751166091685888?s=20 Work life balance: https://twitter.com/hollybik/status/1133751739599876106?s=20 The Monday Motivator - weekly emails that provides positive energy, good vibes, and a productivity tip from the National Center of Faculty Development and Diversity Cal Newport (author of Deep Work) https://www.calnewport.com/about/ @hollybik @ehaswell @baxtertwi @taprootpodcast

111719 Ten Simple Rules Part 9 by revnorman

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Jon and Jeff do a deep-dive into the PLOS: Computational Biology paper "Ten Simple Rules for Better Figures." This one is kind of nerdy, but at this point, isn't that what y'all want?

In Part 5 of our “Moses & Me” series, Rev. Louis Timberlake explores the laws of God and the rules we are called to follow as Christians.

Ped is moonlighting on Tom. If elected, Tom will not serve. They both explore the consequences of convenience, of speeding, of firearms, of bedtime eggs, of sleeping possums, and placebos. Support the podcast on Patreon or by purchasing a T-shirt. Discuss the podcast on Reddit. Review the podcast on Apple Podcasts. Moonlighting Pedcast [00:00] Ped on Two Tims Talking Hello Internet Electoral Tomfoolery [09:29] chuckyc17 on the subreddit is up to some mischief Class Secretary Shermanesque statement Robert’s Rules of Order The Wikipedia is amazing [15:28] Death of a salesman Ten Simple Rules for Editing Wikipedia Reading and Libraries [21:22] Typesetting How Libraries Are Becoming Modern Makerspaces (The Atlantic) Consequences of Convenience [29:44] “The Tyranny of Convenience” by Tim Wu (NY Times) Waiting in Line to See Star Wars: 1977–2000 Humanity’s future according to Wall-E On a Lighter Note: Speeding [41:56] Traffic Warnings How Engineering Decreases Car Crash Fatalities (MrEngineeringGuy) Consequences of Firearms [49:38] The CDC Can’t Fund Gun Research (Wired) Dickey Amendment (1996) Embrace the Cognitive Dissonance - “Put that on a T-shirt” On a Lighter Note: Bedtime Story Eggs [59:23] Peanut Butter Cookies Free-range eggs Consequences of Wildlife [1:06:37] Possums Baby Skunks Baby Mice “This is the part of the job I hate.” (The Simpsons) Consequences of Placebos [1:14:36] Homeopathy is junk science… …but the Placebo Effect is real Music by Lee Rosevere (CC by 4.0)

[fullwidth background_color="" background_image="" background_parallax="none" parallax_speed="0.3" enable_mobile="no" background_repeat="no-repeat" background_position="left top" video_url="" video_aspect_ratio="16:9" video_webm="" video_mp4="" video_ogv="" video_preview_image="" overlay_color="" overlay_opacity="0.5" video_mute="yes" video_loop="yes" fade="no" border_size="0px" border_color="" border_style="" padding_top="20" padding_bottom="20" padding_left="0" padding_right="0" hundred_percent="no" equal_height_columns="no" hide_on_mobile="no" menu_anchor="" class="" id=""] [one_full last="yes" spacing="yes" center_content="no" hide_on_mobile="no" background_color="" background_image="" background_repeat="no-repeat" background_position="left top" border_size="0px" border_color="" border_style="" padding="" margin_top="" margin_bottom="" animation_type="" animation_direction="" animation_speed="0.1" class="" id=""] [fusion_text] In reviewing my Wikipedia page, I noticed some information that is either wrong or narrowly slanted to promote a negative impression of me. We've tried to correct the issue, but Wikipedia makes it very difficult for anyone directly connected to the person or subject of the page to make changes, claiming "Conflict of Interest". This opens the door, however, for those who have a good grasp of editing Wikipedia but who have a decided "left" slant to claim the substance and the direction of the information. Once it's out there, it is very difficult to fix. Wikipedia wants editors to be unrelated to the subject to make contributions. Below are a few things that should be added to the page. Please feel free to make these additions as you would like. Please note that changes need to be discussed in the "Talk" page, first. Here are Ten Simple Rules for Editing Wikipedia. [/fusion_text][/one_full][one_full] [title size="1" content_align="left" style_type="" sep_color="" class="" id=""]On Minimum Wage[/title][fusion_text] Eliminate the minimum wage so teenagers and other low-skill workers can get jobs, and acquire the on-the-job training necessary to earn higher wages in the future. It should not be illegal for people who lack skills to accept employment. The minimum wage punishes workers by legally preventing them from offer their services below a specified legal minimum. If they do not have the skills to justify that minimum, it is illegal for them to work, even if they can offer some value to employers, just not enough to cover the minimum wage mandated by government. So instead of entry level jobs, that will lead to higher skills and wages in the future, many individuals are rendered legally unemployable. People have a right to accept any job that they personally prefer to unemployment. Government should not substitute its judgement for the judgment of workers.[/fusion_text][/one_full] [title size="1" content_align="left" style_type="" sep_color="" class="" id=""]The Housing Market[/title][fusion_text]The government should stay out of housing completely. Abolish agencies that loan money or guarantee mortgages etc. no FHA, Fannie, Freddie. No Department of Housing and Urban Development. There should be a free market in housing. What I actually said on the home mortgage deduction was that, absent the repeal of the entire income tax, we should have a flat rate tax with no deductions including home mortgage, but that would include a much lower rate, so that the removal of the home mortgage deduction did not result in higher taxes. It just eliminates the government- created incentive to buy verses rent.[/fusion_text] Peter Schiff Says The Real Financial Crisis Is About to Hit ... Peter Schiff: When Will the Dollar Bubble Burst? | Wall Street ... The Real Crash: America's Coming Bankruptcy [title size="1" content_align="left" style_type="" sep_color="" class="" id=""]On Gold[/title] [one_full][one_half spacing="yes" last="no"]Peter Schiff's Euro Pacific Bank Introduces a Silver Backed Debit...

Episode 89 - The Lange Money Hour Special: Interview with John Bogle of The Vanguard Group - TOPICS COVERED: Introduction of Guest – John Bogle; Investment vs. Speculation; Profound Conflict of Interests in Money Management Businesses; What Bought on the 2008-2009 Financial Crisis?; Fiduciary Standard and Disclosure of Fees Should Be Enforced; Index Fund Investing = A Winning Strategy; Bogle’s Ten Simple Rules for Investment Success; Don’t Speculate to Get Higher Yields; The Value of Hiring a True Fiduciary Advisor