#ExpertAnswers - an InsideScientific Podcast

Dr. Jack Vanden Heuvel from Penn State University answers questions from a recent webinar discussing the use of cell-based reporter assays for nuclear receptor profiling to efficiently prioritize drug candidates and minimize off-target effects. For more information or to watch the webinar, click here.

Steven Loo-Yong-Kee highlights Artisan Bio's STAR-CRISPR system for optimized gene editing in cell therapy, with a focus on the genetic modification of T cells for cancer immunotherapy.

Sam Sternberg discusses a novel CRISPR-Cas9 system using programmable, RNA-guided transposase and highlighting its implications for genome engineering in cell and gene therapies.

Kaitlynn Arnholt and Elaine Nam discuss how the collaboration between ADInstruments and Vernier Science Education enhances lab-based learning.

Kosuke Minamihata and Jes Kuruvilla discuss how synthetic growth factors work and give application examples using these synthetic peptides for cell therapy and regenerative medicine.

Fan Wu and Lidor Spivak discuss automating electrophysiological data analysis using artificial intelligence and introduced a new cloud-based platform applying these methods.

Nataliia Beztsinna and Marten Hornsveld describe how the tumor microenvironment modulates clinical responses and how patient-derived ex vivo and engineered in vitro models can be used for pre-clinical immuno-oncology testing.

Frauke Greve and Anna Borgström from InSphero highlight the latest advancements in hepatic safety assessment using 3D liver spheroids.

Gethin Evans delves into his experience teaching in the life sciences pre-, during, and post-pandemic and how his department invested in technology to assist with teaching content and delivery during the pandemic.

Jana Kusch, PhD and Marc Demolder, MSc, answer questions from a recent webinar where they discuss their experience with technology-enhanced education.

Patricia Halpin answers questions from a recent webinar where she discusses how she harnesses technology to engage her physiology students in an online clinical pathophysiology course.

Marcel Perret-Gentil presents six simple-to-implement techniques to significantly improve surgical outcomes, to lessen post-op mortality and improve data yield after surgery.

Davide Boido and Antoine Bergel discuss their work on how state-of-the-art multimodal neuroimaging can be coupled with functional ultrasound to study neurovascular coupling across vigilance states.

Jastyn A. Pöpplau and Marilena Hnida discuss their research on prefrontal networks in developing head-fixed mice, and the role of these networks in cognition and models of neurodevelopmental diseases. 

Artur Fedorowski explains CV dysautonomia as a specific sequalae of acute COVID-19 and guides the audience in the diagnostic work-up of PASC patients with suspected cardiovascular complications.

Connar Westgate discusses his research involving intracranial pressure measurements and the importance of understanding this physiological parameter for maintaining normal central nervous system function.

Audrey Dubourg and Gareth Guenigault from CN Bio discuss the value that an organ-on-a-chip approach can bring to drug discovery and development workflows, and how the regulatory landscape is shifting to encourage their widespread adoption.

Sarah Greising discusses the current pathophysiologic understanding of the skeletal muscle remaining following traumatic musculoskeletal injuries.

Marrit Putker, Saskia De Man, and Pirouz Daftarian from Crown Bioscience discuss T cell-directed immunotherapy on the cancer-immunity cycle, including mechanisms and targets capable of modulating and enhancing T cell activity and the application of methods and models including organoids and high content imaging.

Dudley Lamming, PhD, explains that “We Are More Than What We Eat”; Sex and Genetic background are important factors in observing responses to diet in mice.

Phil Griffiths draws from his experience as a telemetry user to discuss some important considerations for designing telemetry studies.

Cole McLarty, Nick Glover and Dr. Timothy Hacker focus on Pressure-Volume Loop collection and data analysis for cardiovascular research.

George Portugal discusses microelectrode array (MEA) technology used for cardiac research and some best practices for integrating this technique into your research protocols.

Dennis Turner delves into dementia syndrome, the metabolic changes that occur, and the importance of proper physiological monitoring of animal models.

Rajendra Kumari and Gera Goverse provide an overview of the cancer immunity cycle and present several methods, techniques, and model systems for immuno-oncology research.

Oscar Perez Leal and Jon Weidanz discuss the basis of the FAST-HDR gene-editing system to create custom cell lines, and present case studies highlighting several applications.

Amy Sheng provides an overview of antibody screening platforms and presents applications and case studies using the Beacon® platform for antibody discovery.

Ejvind Mørtz discusses the benefits and applications of mass spectrometry (MS)-based host cell protein (HCP) analysis and why HCP ELISAs are insufficient for documenting product-related impurities.

Andrew Taylor provides an overview of processes by which drugs are metabolized and talks about the importance of drug metabolism and drug-drug interaction studies.

Giacomo Rossitto answers questions from a recent webinar where he shares his research on heart failure and the related lymphatic dysfunction in patients.

Jonathan Brestoff explains how energy expenditure can be better described thanks to a new bioinformatic algorithm known as Clambake.

Michael Sturek reviews features of macrovascular atherosclerosis and microvascular dysfunction that underlie ischemic events and the need for appropriate animal models for optimal translation.

David Barefield discusses his work investigating the role of the Myosin binding protein H-like (MyBP-HL) protein in atrial dysfunction.

Eduardo Marbán dives into the complexities of regenerative cardiovascular medicine and the future directions for cell therapies.

Jason Rogers and Eliala Salvadori answer questions from a recent webinar that discussed research involving infant emotional communication patterns and coded behaviors.

Bradley Palmer answers questions from a recent webinar where he highlighted and demonstrated the challenging preparation of cardiac tissue slices and measurement of force and calcium.

Adrián Rodríguez-Contreras and Barbara Lind discuss their work on neurovascular coupling in awake head-fixed and anesthetized mice, with a focus on the effects of anesthetics.

Ana Briones discusses her research on the effects and potential therapeutic targets of excessive inflammation and vascular damage in hypertension.

Licy Yanes Cardozo expands on her research exploring the role of androgens on cardiovascular physiology in cis and transgender patients.

Aaron Phillips discusses questions from a recent webinar on his research involving neural hemodynamic control in pre-clinical and clinical models.

Matthew Sparks discusses his latest findings on a system of vascular-epithelial in the kidney that regulates pressure natriuresis.

Valeria Fumagalli and Nancy Kouassi discuss the Syrian golden hamster model of COVID-19, and present a new inhalation exposure-based mouse model using aerosolized SARS-CoV-2. 

Dr. Bonnie Lun from Fortis Life Sciences discusses applications and advantages of using nanobodies for diagnostic and therapeutic research, and highlights Fortis/Abcore's nanobody discovery services.

Peter Ansell and Dr. Marian Navratil discuss the use of novel RNA-based gene expression profiling technologies for clinical and preclinical oncology research.

Louise Naylor focuses on the benefits of exercise to optimize and maintain the cardiovascular system.

Michael Long and Kari Hoffman investigate the neural mechanisms of learning, memory, and behavior using high-density silicon probes from Diagnostic Biochips in small and large animals.

Guto Montezano discusses the role of endothelial inflammation and ACE2 biology in COVID-19 infections and the resulting long-term effects.

Bastiaan Bijl and Dylan Mooijman provide an introduction to single-cell sequencing including potential applications with user cases.

Edward LeCluyse and Paul Gallant discuss their novel all-human hepatic triculture system including its benefits and applications.

Franck Lebrin and Denis Vivien discuss their work on microvascular and functional ultrasound imaging in stroke and neurovascular diseases.

Yuning Chen discusses strategies involved in recombinant antibody production and introduces a novel antibody expression platform to facilitate antibody library generation.