Podcasts about operon

Subdulgence Tracklist: DKK SB81 – Ravens Call Minor Forms, Kublai – Do Remember Zero T, Steo – Stop Start Kublai – No Unity Paradox – Desolator The Sauce – Bang Your Head Dunk – Back To My Roots Break – Something Like That Rider Shafique, Minor Forms – Time Ticking The Sauce, Critical Impact – Grimiest Ever Unglued – Secret Foghorn Total Science – Run It Zero T – Cut Dem Minor Forms, Kublai – Future Motion Toby Ross – Guestlist Sl8r – Sunset Boulevard UFO! – Level Up Yoofee – Freak Hippoflip – No Retreat Ternion Sound – Let Me Out Oxossi – Reflections E s p – Open Mind Sling Wave – Juggernaut Ternion Sound – Scrambled Abstrakt Sonance – Elephant War Dub Cesco – Juan40 Hippoflip – Haha Ok Toast, Unkey – Focusing Roklem & Sebalo – Moving Forward Operon 1. ‘one big puff' - Neo-Geo (flight pattern) 2. ‘slipped' - Motiv (innerground) 3. ‘take you there' - Chase & Status (ram) 4. ‘contrast' - Operon (8ball audio) 5. ‘deep blue (Myth remix)' - NC-17 (rebel music) 6. ‘take my breath away' - Paul T, Serum & Edward Oberon (V-Recordings) 7. ‘tekapo' - Unglued (hospital) 8. ‘out of air' - Motiv (fokuz) 9. ‘INO' - Talix (onesevenfour) 10. 'time to think of something better' - Mage (fokuz) 11. ‘little treasures' - Random Movement (liquid V) 12. ‘alone' - Surreal (fokuz) 13. ‘got no trouble' - operon (8ball audio) 14. ‘sunglasses' - Motiv & Surreal (innerground) 15. ‘do it (random movement remix)' - Surplus (saucy vibes) 16. ‘ditty bitty' - Random Movement (flightpattern) 17. ‘wild ride' - Dava (together with Ukraine) 18. ‘never stop (flowrian remix) - Enea (beatalistics) 19. ‘zodiac' - Break (symmetry) 20. ‘come & reprazent' - Zero T (symmetry) 21. ‘tamtoumb' - Dedman (rebel music) 22. ‘cure me feat' lolo (dj die remix)' - Redlight (polydor) 23. ‘come in and swing' - Operon (unreleased dub) 24. ‘the test' - Urbandawn (together with Ukraine) 25. ‘guilty pleasures' - Saikon (innerground) 26. ‘Bristol' - Technimatic (shogun audio) 27. ‘falling feat' Chimpo)' - Slay & Sl8r (V-recordings) 28. ‘visions' - Mackadena, Emery & Dreazz (fokuz) DKK Bakey – Aftershun Dub

Welcome Back to Breaking Beats Drum and Bass Mixshow Episode 61. Got Another Packed show with some Fine Drum and Bass Tunes included from the likes of Rueben, Operon, Break, Prestige, DJ Marky, Dub Phizix, Screamarts, Dawn Wall, Chase & Status and Disaszt to name a few. Also as mentioned were performing our first Live Gig all details can be found here: https://www.youtube.com/redirect?event=video_description&redir_token=QUFFLUhqbFgtcERvdTJHTFdzX3RfdWlDY0RiZkNyTVZGZ3xBQ3Jtc0ttS0RKMjF2V1IxZjFVY19hRXEtT1FPZmpYenhzVS1JQUlLZ2hxekVfblhydWFiZExJWXMyM2ktdDhRMy1JQjdCcXBySGRVNWtYSmdyT1VYQnQ5eEZnSmU0SGtocnI4R18zM0hXWmllYzhxRWxLU0xnMA&q=https%3A%2F%2Fwww.skiddle.com%2Fwhats-on%2FLondon%2FELECTROWERKZ%2FLONDON-ELECTRONIC-ARTS-FESTIVAL%2F36171150%2F&v=X4xN7Qsz0_w So come along say hi :) Hope you enjoy this episode

Lux Operon joins Taebyn this week as co-host. We make terrible jokes, learn about ring-tailed lemurs in Lux's Better Know a Species, hear some That's Just Stupid stories from Rayne, Lux dusts off the portable transfurmation ray to run an experiment on Taebyn, and then Taebyn gives us a stellar math lesson. All this and more in this episode of Bearly Furcasting Feat. Taebyn!Support the show

Unser Kurs auf der Webseite: https://www.selbstorientiert.org/shop/Genregulation-I-Kurs-mit-Karteikarten-&-Texten-p429173265 Unser Kurs auf Amazon: Unser YouTube-Video: https://www.youtube.com/watch?v=bvZMjijl_ag --- Send in a voice message: https://anchor.fm/selbstorientiert/message

Unser Kurs auf der Webseite: https://www.selbstorientiert.org/shop/Genregulation-I-Kurs-mit-Karteikarten-&-Texten-p429173265 Unser Kurs auf Amazon: Unser YouTube-Video: https://www.youtube.com/watch?v=bvZMjijl_ag --- Send in a voice message: https://anchor.fm/selbstorientiert/message

Part 2 of 2 - Dr. Marcus Curcija continues the discussion with Jane Vadiveloo, Founding CEO and William Tilmouth, Founding Chair of Children's Ground. Jane and William have worked together for over 15 years, culminating in the foundation and direction of Children's Ground. Children's Ground is an organisation led by Aboriginal communities, creating a new future for children filled with promise, justice, hope and empowerment. Children's Ground works with children, families and communities that face the greatest exclusion and live with injustice and disadvantage every single day. We unpack how Stakeholder Engagement and Corporate Social Responsibility can empower First Nations people and ensure that the economic, cultural, and social justice first nations people is upheld and promoted. William Tilmouth was born in Mparntwe (Alice Springs) and is an Arrernte man. A member of the stolen generations, William was taken to Croker Island where he lived and attended school with many other children sent there as a result of Government policy of that era. Insight Into Impact connected with Children's Ground after watching In My Blood it Runs. A documentary which arose from the work and families who lead Children's Ground. The documentary follows the story of ten-year-old Dujuan, a child-healer, a good hunter and speaker of three languages. As Dujuan shares his wisdom of history and the complex world around him, the audience witness his spark and intelligence. Yet Dujuan is ‘failing' in school and facing increasing scrutiny from welfare and the police. As he travels perilously close to incarceration, his family fights to give him a strong Arrernte education alongside his western education lest he becomes another statistic. His grandmothers lead Children's Ground and key advisors to the film include the Chair, CEO and cultural leaders of Children's Ground. We encourage you to follow the link below and watch the documentary. We also encourage you to follow the work on Children's Ground and learn how you can support both these organisations. Childrens Ground: https://childrensground.org.au/ In My Blood It Runs: https://inmyblooditruns.com/ Insight Into Impact: https://insightintoimpact.com.au/ Insight Into Impact acknowledges and honours First Nations peoples, paying respect to Elder's past and present as custodians of this land. Music by Operon

Part 1 of 2 - We welcome Jane Vadiveloo, Founding CEO and William Tilmouth, Founding Chair of Children's Ground. Jane and William have worked together for over 15 years, culminating in the foundation and direction of Children's Ground. Children's Ground is an organisation led by Aboriginal communities, creating a new future for children filled with promise, justice, hope and empowerment. Children's Ground works with children, families and communities that face the greatest exclusion and live with injustice and disadvantage every single day. We unpack how Stakeholder Engagement and Corporate Social Responsibility can empower First Nations people and ensure that the economic, cultural, and social justice first nations people is upheld and promoted. William Tilmouth was born in Mparntwe (Alice Springs) and is an Arrernte man. A member of the stolen generations, William was taken to Croker Island where he lived and attended school with many other children sent there as a result of Government policy of that era. Insight Into Impact connected with Children's Ground after watching In My Blood it Runs. A documentary which arose from the work and families who lead Children's Ground. The documentary follows the story of ten-year-old Dujuan, a child-healer, a good hunter and speaker of three languages. As Dujuan shares his wisdom of history and the complex world around him, the audience witness his spark and intelligence. Yet Dujuan is ‘failing' in school and facing increasing scrutiny from welfare and the police. As he travels perilously close to incarceration, his family fights to give him a strong Arrernte education alongside his western education lest he becomes another statistic. His grandmothers lead Children's Ground and key advisors to the film include the Chair, CEO and cultural leaders of Children's Ground. We encourage you to follow the link below and watch the documentary. We also encourage you to follow the work on Children's Ground and learn how you can support both these organisations. Childrens Ground: https://childrensground.org.au/ In My Blood It Runs: https://inmyblooditruns.com/ Insight Into Impact acknowledges and honours First Nations peoples, paying respect to Elders past and present as custodians of this land. Music by Operon

Bearly is gone this week, but Lux Operon fills in. Special guest Strongbob Fox joins Taebyn and Bearly on a pre-recorded interview. Taebyn explains Scrutinatinty. What does Cilantro taste like? Did this Episode stay on the tracks or was it off the rails? Why was Bearly the subject of so many bad jokes? Do Theraminists get angry? This episode is stranger than most, so tune in and give us a listen! You won't be disappointed. (we hope)Support the show (https://ko-fi.com/bearlyfurcasting)

As explored previously on the Insight Into Impact Podcast, waste management is a pressing concern facing communities across the globe. Although several locations are using technology and innovative techniques to tackle this challenges, unfortunately, there are many local councils and municipalities that are still seeking to better understand the problem and find scalable solutions that will improve land management and resource allocation. Enter Emma Avery, who has made it her personal mission to mitigate waste and save families money by educating and empowering caregivers to better understand the benefits of cloth nappies. Her mission does not stop there, as she also engages with public and private entities so they support and implement education and infrastructure that helps to reduce the volume of waste headed to landfills. Emma Avery is a waste and sustainability professional with over 10 years experience in local and state government delivering community sustainability education programs. In 2019, she founded Cloth Baby | modern cloth nappies, filling an education gap to help parents avoid disposable nappy waste. From this, she realised that there was more to be done in the field of waste education and established her consulting business EA Sustainability, working with community groups and government to communicate new changes in waste management. Emma runs workshops for new parents to understand modern cloth nappies so that they start and continue to use reusable nappies for their baby in a supported environment. She sees the bigger issue with disposable nappy waste and believes that the education for using reusables is only the beginning of what can be achieved to avoid disposable nappy waste to landfill. In addition to waste management, this episode also reveals the power of innovation and stakeholder engagement when faced with a pressing challenge. Emma details how she looked inward to uncover the passion, motivation, and ingenuity needed diagnose a waste management solution that can be promoted and scaled nationally. Music by Operon

For this episode, Angus talks about his experience promoting geotourism, which really overlays with so many of the underlaying fundamentals of this podcast – stakeholder engagement & management, understanding the perspectives of community members, measuring impact, and working with various stakeholder groups to achieve a desired outcome. An exploration geologist by profession, Angus has conducted tourism marketing, business management, and lobbying services as founder (1993) of Leisure Solutions. He also serves as Coordinator, National Geotourism Strategy for the Australian Geoscience Council and as a member and inaugural chair of the Geotourism Forum of Ecotourism Australia. After 25 years working in information and communication technology and manufacturing industry development roles in an executive capacity, he has been engaged in ecotourism activities in Queensland's Scenic Rim as an eco-certified tour operator as well as developing geotourism in the Red Centre, Blue Mountains and Flinders Ranges national landscape areas. Due to this experience, Angus has worked with all sorts of stakeholder groups in order to have lands recognised for their value. This includes going to community meetings, meetings with industry representatives to discuss how geotourism and heritage preservation can impact their business, and it also includes dealing with many mining businesses so they understand and appreciate the historical value of the ground they may be working on. Throughout this episode, Angus discusses the barriers and challenges he has experienced and what he has done in response to bring positive impact to the community members located within the destinations he has evaluated. Music by Operon

Cameron Price connects, informs, and inspires social entrepreneurs as the convenor of the Social Enterprise Community. Based in Melbourne, Australia, the Social Enterprise Community is a tribe of people who are passionate about using for-purpose businesses to drive social impact. It is made up of members from across the world who are change-makers and social entrepreneurs seeking to evaluate and increase the positive environmental and social impact of programs. The Community also supports members by teaching them how to establish or scale-up their social impact venture. The Social Enterprise Community regularly offers a Masterclass – where leading social enterprise experts discuss their work and how they are assisting other social enterprises to be more innovative and market ready. The Community also features a Roundtable that invites all members receive mutual support, discuss shared experiences, and the exchange advice. In summary, members band together to learn from each other's' mistakes, collectively troubleshoot challenges, and explore opportunities to collaborate. For more information, please reach out to Cameron Price on Linkedin: https://www.linkedin.com/in/cameronpr1ce/ To join the Social Enterprise Community visit: https://www.socialenterprise.community/ Music by Operon

At our core, the Insight into Impact Podcast is about preparing business owners and managers with the knowledge they need to implement principles and practices that will enable their organisations to be more sustainable and achieve greater social or environmental impact. Therefore, every week, we feature a new guest that can provide in-depth, specialised input on a topic that can be integrated into strategy and operations. For this episode, we have invited Indi Tansey, who has owned and operated her own tech business, which supported the development of innovative sustainability tech solutions. Over the past few years, Indi has become a huge advocate for the Sustainable Development GOALS (SDGs) and now actively encourages every business to do what they can to make a difference and generate a social & environmental impact. Her involvement with the SDGs has also seen her become involved with young professionals and artists that are using art to communicate and promote the future they want. This art exhibition is not to be missed and will resonate with all members of the audience. Indi is also the Director at Women in Technology which is an informative and supportive environment for women and young ladies looking to make their mark/ get involved in science, technology, engineering, and mathematics – fields that have historically been dominated by men. Overall, this Insight into Impact episode explores Indi's journey from initially trying to make her mark in tech to now being being a leading voice in sustainable development and the adoption/ integration of the SDGs into everyone's life / best business practice. Here are some links that Indi references throughout her interview: Invisible Women book UNAA QLD facebook page for SDG art exhibition GapSquare - gender pay gap technology Impactr - launching early 2021 Everledger - blockchain supply chain transparency Eugene - genetic testing got boosting female founders grant Teachstarter and their green futures club SDG action manager EcoBiz - CCIQ program for business sustainability WiT board readiness course Indi's blog about Women in Technology Indi's LinkedIn Profile: https://www.linkedin.com/in/inditansey/ Music by Operon

The purpose of the Insight into Impact Podcast is to inform individuals about how they can improve their business by adopting more sustainable principles and practices. Every episode we talk about steps and insights that will improve business acumen and result in increased economic prosperity. For our fifth episode, we brought in Rosemary Addis to talk about the Sustainable Development GOALS, impact measurement and management, impact investing, and impact-washing. Although these topics have all been previously mentioned, eluded to, and discussed on the Podcast, Rosemary brings in a higher view to tie everything together and explain what these topics mean to the business world, no matter of size or capacity. She also goes into detail about what everyone can do to implement sustainable principles and practices and make a difference. Rosemary's work has been influential. Her insights have helped guide enterprise, bond, and investment decision-making across the world. For those seeking to better understand sustainability and what it means to them and for those who have struggled to understand the ‘bigger picture', this episode is not to be missed. Linkedin: https://www.linkedin.com/in/rosemary-addis-am-94a1bb15/ Music by Operon

Stakeholder engagement and management is an essential aspect of doing business. As such, this episode features Andres Recalde, Director at Kuya Silver. Andres is an in-demand stakeholder engagement and management specialist for the mining and extractive industries across Latin America. Within this episode, Andres will discuss the importance of ‘social license' and why it is essential for companies to be a ‘good neighbour'. Andres also discusses how companies must be prepared to use their resources to build trust and ensure they back-up their promises. Understanding common goals between companies and the community is the first step in ensuring dialogue and a productive relationship between the two entities. He also details his work resettling entire villages and the importance of understanding the perspectives of all community members and ensuring that everyone is heard. Linkedin: https://www.linkedin.com/in/arecalde/ Social Practice Forum: www.socialpracticeforum.org Music by Operon

More and more, the ‘circular economy' is being discussed as a solution to climate change, resource management, and economic disparity. Many businesses are implementing circular economy strategies into their operations and there is a good chance that your local council or community organisation is talking about how to integrate circular economy practices into your local recycling, waste management, or other resources-based process. However, one of the main problems around ‘circular economy' is the amount of confusion that surrounds it. So, Insight into Impact has invited a circular economy advocate, spokesperson and educator onto the show to clear any misunderstandings. As the Founder of Circular Economy Pioneers Australia, Chelsea Mclean brings enthusiasm for all things circular economy. Throughout the episode, she discusses what the circular economy is, what the benefits are, where it originated, and what frameworks are most pertinent to certain markets. Chelsea also gives details about how ‘circular' Australian and circular economies are at the moment Various circular business models are explored, along with each's pros and cons. Chelsea provides real-world examples for each and discusses some of the issues/ challenges that impact their ability to be integrated at scale. Linkedin Profile: https://www.linkedin.com/in/chelseamcleanprandmarketing/ Music by Operon

The journey of this Podcast series is to uncover how stakeholders can achieve more sustainable results. To this end, for the second episode, it was appropriate to explore ‘resilience' because as a society we are currently looking to emerge from this pandemic with a new, more profound appreciation for leadership and recovery practices. Many communities across Australia and in Asia have called upon Leigh Bennet, Executive Director, Leadership Solutions Consultant and Facilitator for The Institute for Tourism Leadership Australia, to design and deliver their resilient leadership and mentoring capacity building programs. Through his guidance, these communities are emerging stronger than prior to the pandemic because now they have the tools to overcome adversity and recover personally, organisationally, and as a community. In this episode, Leigh discusses how in order to achieve the sustainable outcomes that we desire as a society, as an organisation, or even personally, we need to be able to recover – to “bounce back” from issues and challenges in a productive, meaningful way so that those around us are inspired to focus on positive impact solutions. Leigh also explores his integrated approach to understand, assess, and build personal resilience, which includes the ability to recover and respond with commitment and optimism.. In addition to being a highly sought after Resilience mentor, Leigh was asked to be come onto the Podcast because of his keen ability to speak very thoroughly and knowledgeable about a subject. Listening to this show, it will become evident as to why Leigh has made a career out of facilitating outcomes for councils, organisations, and stakeholder groups. Find Leigh on Linkedin: https://www.linkedin.com/in/leighbennett1/ Email: leigh@tourismleadership.com.au Music by Operon

For our first episode, we unpack risk. Risk was identified as a topic our listeners should be familiar with as we progress through this podcast series. Understanding the need to gather perspectives and identify issues and challenges is integral to risk management, sustainability evaluation, and impact measurement practices. Therefore, by better understanding risk and how to manage risk, a logical conclusion can be drawn that outcomes can become more sustainable and impactful for intended beneficiaries. As Head of Risk, Bupa Asia, Rodney Gollo joins to the podcast not in an official capacity, but as a risk practitioner who has experience managing risk for companies. Rodney reviews different ways of managing risk and dives into how organisations can mitigate risk by having systems in place to better understand perspectives. Being prepared and responsive is a form of risk management that helps organisations simplify the complexity of the world we are living in today. At the end of the podcast, Rodney presents a checklist for both companies and investors to better appreciate and manage potential risks associated with ESG, or environmental, social, and governance, factors. Find Rodney on Linkedin: https://www.linkedin.com/in/rodney-gollo-20083315/ Music by Operon

This week we chat with Lux Operon and learn about bioluminescence. Listener Mail includes some packages and Halloween takes over the podcast. Come waste time with Bearly and Taebyn. You might have fun!Support the show (https://ko-fi.com/bearlyfurcasting)

More terminology of the Lac Operon mechanism, refined ideas from the first part.

Discusses why gene expression regulation occurs @ transcription level. Discusses the Jacob Monod Lac Operon's structure (ZYA) and function (lactose metabolism) and how the repressor (@operator site) controls this process.

Listen to the special guest mix from Operon (Golden Orb Records, Offworld, Fokuz Recordings, Influenza Media UK, Camino Blue Germany, Santorin 2000) https://soundcloud.com/operon

Listen to the special guest mix from Operon (Golden Orb Records, Offworld, Fokuz Recordings, Influenza Media UK, Camino Blue Germany, Santorin 2000) https://soundcloud.com/operon

A teaching assistant explains the lac operon, using props to demonstrate how the lactose metabolism machinery works.

The D.R.U. Show #9 - 13/Jul/2009 Tracklist: Mr. Distance & Joe Syntax - Void [Hustle Audio dub] Linden - Sabotage [Broken Audio dub] Decem & Glen-E-Ston - Star City Saga [dub] Operon ft. Manta Ray - Airports Division - Deep Roots Scenic & Advisory - Don't Tell Me [dub] Lenzman - Marseille Crix & D-fect - All I Ever Needed [dub] Brooklyn - Someone (Naibu Remix) Undersound - Into The Woods [dub] Simplifciation & Edward Oberon - Loveline [Digital Blus dub] LJ High - Trouble (rework) [dub] Loxy & Isotone - Ancients Fre4knc - The Tube Druid - Lightheaded [dub] Proxima - Serengeti Raiden - M82 London Elektricity - Just One Second (Apex Remix) Fourward - Lucid dreams LAOS - Everything Deadmau5 - I Remember (J Majik & Wickaman Remix) Druid - Burning [dub] Camo & Krooked - Get Funky LAOS - We All Druid - The Power [dub] Random Movement - Her Song Syncopix - Nightlistener Glamour For Better - Architeks Of Disco (John B Remix) Druid - The Blue Yonder [Deep Instinct dub] Greg Packer - Meltdown Redeyes - Poetry In Motion Zero T - Why Would You

The D.R.U. Show #9 - 13/Jul/2009 Tracklist: Mr. Distance & Joe Syntax - Void [Hustle Audio dub] Linden - Sabotage [Broken Audio dub] Decem & Glen-E-Ston - Star City Saga [dub] Operon ft. Manta Ray - Airports Division - Deep Roots Scenic & Advisory - Don't Tell Me [dub] Lenzman - Marseille Crix & D-fect - All I Ever Needed [dub] Brooklyn - Someone (Naibu Remix) Undersound - Into The Woods [dub] Simplifciation & Edward Oberon - Loveline [Digital Blus dub] LJ High - Trouble (rework) [dub] Loxy & Isotone - Ancients Fre4knc - The Tube Druid - Lightheaded [dub] Proxima - Serengeti Raiden - M82 London Elektricity - Just One Second (Apex Remix) Fourward - Lucid dreams LAOS - Everything Deadmau5 - I Remember (J Majik & Wickaman Remix) Druid - Burning [dub] Camo & Krooked - Get Funky LAOS - We All Druid - The Power [dub] Random Movement - Her Song Syncopix - Nightlistener Glamour For Better - Architeks Of Disco (John B Remix) Druid - The Blue Yonder [Deep Instinct dub] Greg Packer - Meltdown Redeyes - Poetry In Motion Zero T - Why Would You

Hallo und herzlich Willkommen zur 13ten Folge unseres AbiTour Podcasts. Heute beschäftigen wir uns mit dem Thema Genregulation. Wir werden uns die Genregulation bei Prokyroten anschauen und beide Modelle (lac-Operon und trp-Operon) intensiv besprechen.Diese Folge wurde ebenfalls von Rosanna und Mathias geschrieben, also Vielen Dank nochmals an dieser Stelle! Viele Grüße Olli & Sven.

In dieser Arbeit wurde die Methode der subtraktiven Hybridisierung angewandt, um neue Virulenzfaktoren zu finden, die spezifisch für hochpathogene Yersinia enterocolitica Stämme sind. Hierfür wurde die DNA eines nicht pathogenen „Treiber”-Stammes (Y. enterocolitica NF-O, Biotyp 1A) gegen die DNA eines hochpathogenen „Tester”-Stammes (Y. enterocolitica WA-314, Biotyp 1B) subtrahiert. Mit Hilfe der subtraktiven Hybridisierung konnten verschiedene Tester-spezifische Sequenzen ermittelt werden, die sowohl für bereits bekannte als auch neue potentielle Virulenzmarker kodieren. In dieser Arbeit konnte ein neues TypII-Sekretionscluster, genannt yts1 (Yersinia TypII Sekretion 1), ermittelt werden. Das yts1-Gencluster umfasst ein 13 kb großes Operon-ähnliches Modul, welches die Gene yts1C-S enthält. Mittels reverser Transkription/PCR konnte eine bevorzugte Transkription bei 37 °C gezeigt werden. Southern Blot-Analysen sowie PCR haben gezeigt, dass das yts1-Gencluster nur in den hochpathogenen Y. enterocolitica Stämmen vorkommt. Dagegen sind yts1-Gene weder in schwachpathogenen sowie apathogenen Y. enterocolitica Stämmen noch in Y. pseudotuberculosis- und Y. pestis-Isolaten zu finden. Durch Inaktivierung des yts1E-Gens in Y. enterocolitica wurde eine Mutante hergestellt und hinsichtlich Mauspathogenität mit dem Mutterstamm verglichen. Bei oraler Infektion der Mäuse erwies sich die yts1E-Mutante als attenuiert (geringere Keimzahlen) in Leber und Milz im Vergleich zum Mutterstamm. Im Gegensatz dazu konnte bei intravenöser Infektion der Mäuse kein Unterschied zwischen Mutante und Mutterstamm festgestellt werden. Dies könnte ein Hinweis darauf sein, dass das TypII-Sekretionssystem die Erregerdissemination von den Peyer-Plaques in Milz and Leber fördert. Das yts1-Sekretionscluster grenzt stromabwärts an ein Gen, welches für ein potentielles Chitin-Bindungsprotein (ChiY) kodiert. ChiY ist ein mögliches Substrat des Yts1-Sekretons. Sequenzanalysen sagen voraus, dass ChiY ein 55-kDa Protein mit zwei definierten Chitin-Bindungsdomänen ist, von denen sich die eine Domäne am N- und die andere am C-Terminus des Proteins befindet. Es konnte gezeigt werden, dass rekombinantes ChiY Chitin bindet. Sequenzanalysen des zugänglichen fast kompletten Genoms von Y. enterocolitica 8081 (Biotyp 1B) führten zum Nachweis eines möglichen zweiten TypII-Sekretionscluster, das in dieser Arbeit als yts2 bezeichnet wird. Wie mittels PCR gezeigt werden konnte, kommt yts2 - im Gegensatz zu Y. pseudotuberculosis und Y. pestis - in allen getesteten pathogenen und apathogenen Y. enterocolitica-Stämmen vor. Reverse Transkriptionsanalysen/PCR zeigten, dass die yts2 - Gene bevorzugt bei 27 °C abgelesen werden. Mittels subtraktiver Technik konnte auch ein neues Insertionselement (IS1330) charakterisiert werden. Durch Southern Blot-Analysen konnte gezeigt werden, dass IS1330 nur in pathogenen Y. enterocolitica Serotypen vorkommt und somit für die epidemiologische Typisierung dieser Spezies eingesetzt werden kann. Diese Arbeit repräsentiert einen neuen Ansatz zur Aufklärung von unterschiedlichen intraspezifischen Genomsequenzen von Y. enterocolitica mit Hilfe der subtraktiven Hybridisierung, um unser Verständnis der genetischen Vielfalt und Heterogenität dieser bakteriellen Spezies zu erweitern. Das zum ersten Mal hier beschriebene yts1-Cluster repräsentiert einen neuen Lokus, der eine wichtige Rolle für die Pathogenese der hochpathogenen Y. enterocolitica Stämme spielt.