Podcasts about aeromonas

Fishhook injuries are common, surprisingly nuanced, and honestly a little intimidating until you've removed a few. In this first episode of our Minor Procedures series, we'll reel in the essentials of pediatric fishhook removal, helping you take the bait on four classic removal techniques, procedural planning, anesthesia strategies, and post-removal management. We'll discuss when to pull back, when to advance, when not to get hooked on a single technique, and how to avoid turning a simple procedure into the one that got away. Along the way we'll cover sedation, antibiotics, wound care, and practical pearls to help you land these cases with confidence. Learning Objectives Compare and select among the four major fishhook removal techniques based on hook characteristics, depth of penetration, and anatomic location. Apply evidence-based approaches to analgesia, anxiolysis, procedural sedation, and post-removal management for pediatric fishhook injuries. Identify situations requiring escalation of care, including ocular involvement, contaminated water exposure, tendon or joint involvement, and circumstances where routine management may not be sufficient. References Gammons MG, Jackson E. Fishhook removal. Am Fam Physician. 2001;63(11):2231-2236. Prats M, O'Connell M, Wellock A, Kman NE. Fishhook removal: case reports and a review of the literature. J Emerg Med. 2013;44(6):e375-e380. doi:10.1016/j.jemermed.2012.11.058 Doser C, Cooper WL, Ediger WM, et al. Fishhook injuries: a prospective evaluation. Am J Emerg Med. 1991;9(5):413-415. doi:10.1016/0735-6757(91)90204-w Transcript This episode used an AI-generated transcript created in Descript as an initial draft. The transcript was subsequently edited, expanded, and refined by the author with assistance from OpenAI's ChatGPT (GPT-5.5). Final editorial decisions and content responsibility remain with the author. Welcome to PEM Currents: The Pediatric Emergency Medicine Podcast. As always, I'm your host, Brad Sobolewski, and today we're gonna start a new series on minor procedures. These are the types of procedures that we perform all the time in the emergency department. They're not the subject of multicenter trials or big keynote lectures, but these are the things that patients and families remember, and trust me, they will remember them whether you do them well or not. First up, fishhook removal. So I'm hoping to reel in some listeners with this one, and so hopefully you'll take the bait, and by the end of this episode you'll understand exactly what angle I'm coming from. And hopefully I'm just not trying to make a bass of myself. So anyway, fishhook removal sounds really simple until you actually start doing it. There's not just one technique. There are four classic approaches, and I'll talk about them all, and which one you choose depends on the hook, whether there's a barb, how deep it is, where it's located, your personal experience with different techniques. Fishhook injuries in children are usually minor and most commonly involve the hands and head, though I've seen them stuck in other body parts as well. Most can be managed in the emergency department or urgent care setting with local anesthesia and basic equipment Of course, if there's concern for tendon involvement, joint penetration, neurovascular compromise, if it's anywhere near the eyeball, you should stop and rethink your plan. You know, so ortho, if it's embedded deeply in a joint, um, anything that involves the eye itself isn't necessarily an emergency department procedure, and I'm not talking about the eyebrow, I'm talking about the globe. Fortunately, that's very rare, but that's definitely an ophthalmology conversation. And so before you even think about removing, you need to understand the hook. Is this a single hook or is this a treble hook? A treble hook is a type of fishing hook that has three individual hooks and barbs arranged in a triangular formation, and they're all fused to a single shank and eye. The eye is where the line gets tied to the hook. Is it freshwater or saltwater? How long has it been there? Is it an old rusty one that was sitting in your garage? Was it underwater for a few hours and then it got hooked in the skin? And honestly, how cooperative is the kid gonna be? Because unlike actual fishing, this is one of the procedures where patience beats blunt force. So the simplest technique is retrograde removal. This is exactly what families think you're gonna do before you walk in the room. You know, just pull it out the way it went in. But that's not how hooks are designed. They have the barb. They're designed to stay in the fish. So most of the hooks that I've removed are barbed hooks, and so you can't just back them out. If you try to pull a hook out the way it came in, it's gonna catch and tug on the tissue, it's gonna lead to more pain, bleeding and tissue distortion and not really gonna get you anywhere. So just pulling it out doesn't work, and family probably would have already tried that at home. The technique I end up using most often is advance and cut. And it kind of sounds wrong the first time you explain it to a family because your solution to removing the hook is to continue to advance the hook, but mechanically, this makes the most sense. So you advance the point of the hook through the skin until the barb exits completely, then use either really good trauma shears or heavy wire cutters to cut the hook in between the shank and the barb. If it's in a location where you have, uh, enough room, I like to hold a hemostat real close to the skin, grabbing the hook. Then I cut near the barb, get the pointy part out of the way, remove the hemostats, and then back it through the skin. This is considered the most reliable technique, and in most reviews it's described as being nearly universally successful, even for larger hooks. In children, I think this needs to be the go-to technique because success matters. You just gotta get it done on the, the first attempt. Kids don't tolerate multiple failed attempts very well. Um, obvious downside is that you create a second puncture wound, but in practice, that puncture is usually controlled and much less traumatic than repeated unsuccessful pulling. Depending on where the skin's at, you may actually need to put a little bit of tension or pressure against the skin to get that hook to poke through. Ultimately, this advance and cut method is the one that you should spend the most time learning and teaching to your trainees. The string yank technique is the one that often is seen at summer camps and on YouTube videos. You loop string or heavy suture or even fishing line around the bend of the hook, apply downward pressure to the shank to disengage the barb, and then pull quickly in line with the shaft of the hook. When it works, it yanks it out almost instantly. That's why the YouTube videos are popular. One second there's a fishhook in the finger, and the next there isn't. The advantage is that this can sometimes just be performed without anesthesia and can even be done at home. The disadvantage is obvious if you work with children. This requires cooperation. Younger kids, anxious kids, a treble hook, something that's deeply embedded, like this isn't gonna work all that well, and it's, again, less reliable with bigger and deeply embedded hooks. The last technique is needle cover. This one gets less attention. It seems elegant, but in practice it's actually pretty hard to do, especially in smaller kid parts. You insert an 18-gauge needle alongside the entry tract until the bevel of that needle covers the barb, and then pull both out together The advantage is that you avoid creating a second puncture wound, and you can minimize tissue trauma. The disadvantage is it's really complex technically. Maintaining alignment of both the hook and needle can be tricky because they sort of like roll and move around. And if you want to do this one, it's probably easier for smaller and medium-sized hook rather than larger embedded or treble hooks. And as you might imagine in the literature, there's not really any randomized trials comparing these techniques. Most of what we know comes from prospective observational studies, case series, procedural experience, and expert review. Advance and cut seems to have the broadest success across scenarios. String yank does earn some points for field use and avoiding local numbing. Needle cover is hard to do, but if the parent is absolutely adamant that you don't create a second hole, then that's probably your best option. And as with any procedure, you should probably be facile in multiple techniques in case the first one doesn't work. You don't just want to stand there and flounder. Anyway, most fishhook removals in children can be done with local anesthesia alone. One percent Lido with or without epi is usually enough. Depending on the location, you may need to do a digital block or a field block instead of just injecting directly around the hook because local infiltration itself can distort the anatomy and actually make removal harder. So that's why I like blocking the digit or doing a little bit of a field block around it. If you have time, a topical anesthetic before local infiltration can be a nice gesture. LMX or EMLA can be really helpful, especially for really anxious kids or kids who are escalating before you even start setting up. They take about forty to sixty minutes. About forty-five minutes is probably ideal. So if you can get that put on in triage, that's actually a, a great technique. So if you know you're going to inject to numb to get the fishhook out, and you need a little bit of extra time to get child life or other personnel in the room, by all means, put a topical anesthetic there. It only absorbs into the outer two millimeters, but it'll help with the poke, not necessarily the burning that happens once the lidocaine is in the tissue. And now that we've talked about pain, I think it's also important to talk about anxiolysis. Most kids that have embedded fishhooks don't need full procedural sedation. If it's right next to the eye, like in the eyelid, then that might be beneficial, especially in a preschool-aged kid or younger. Plenty of them do need some anxiolysis. Um, intranasal or oral midazolam is probably, uh, the most popular option. It's got rapid onset in about twenty minutes, no IV, some amnesia. Recent pediatric data suggests that point four or point five milligrams per kilogram may perform better than lower doses, uh, for the intranasal. If you've got nitrous oxide, that's another nice option for cooperative kids. It provides anxiolysis and analgesia with rapid recovery and a very low rate of adverse respiratory events. Fishhook removal is actually one of those procedures where nitrous can feel disproportionately helpful because the procedure itself is often quick, and the hardest part is just reducing the fear and helping the kid hold still for about thirty to sixty seconds. I think ketamine still has a role. I alluded to when I might use that earlier. Occasionally, you walk into the room and then there's a deeply embedded treble hook, a really anxious child, a failed attempt prior to you being there. And ultimately, yes, IV procedural sedation with ketamine should be on the table, and it's as always an excellent option. And never, ever underestimate distraction. Hopefully, you work in a place where there are child life specialists because they are wonderful. They are magic. But you've got videos, you know, music, VR, parents. I mean, sometimes the difference between success and failure is a working iPad. And then finally, the question of antibiotics. So fishhook removal does not automatically equal a course of antibiotics. A prospective series of one hundred fishhook injuries found prophylactic antibiotics were unnecessary for uncomplicated soft tissue injuries that didn't involve the cartilage or tendon. So if you've got a contaminated wound, a delayed presentation, you know, it was already in an established infection, though I've never actually seen someone impale a fishhook into an area of cellulitis. There's tendon involvement, joint involvement, or, you know, gross water exposure. Well, then maybe consider antibiotics. Freshwater injuries do raise concern for organisms like Aeromonas. Saltwater injuries introduce concern for Vibrio species and occasionally Mycobacterium marinum enters the conversation or the tissue. Um, saltwater injuries are often treated with doxycycline plus a third-generation cephalosporin. You recognize the doxy decisions in younger children require some additional consideration. Freshwater injuries could push you towards broader Gram-negative coverage, but, but honestly, for most fishhook injuries, especially in healthy children, you're just dealing with skin flora. So once I get the hook out, I make sure there's no other retained foreign bodies, like little pieces of the hook or little pieces of the barb. I irrigate with saline or tap water, maybe a hundred mLs for a smaller hook, more for bigger hooks or grossly contaminated wounds. Make sure that there's full neurovascular function and normal range of motion. Antibiotic ointment, simple dressing, update their tetanus shot if it's not been within five years, and explain to the family that the good news is that this is really a forgiving injury most of the time. Once the hook is out, these generally heal really well. We don't need to suture them back up. We're not worried about long-term damage. Tell the parents to watch out for increasing redness, worsening pain, pus drainage, fever, or other systemic symptoms, trouble moving the area, especially if it was around a digit, you know, numbness or anything else that makes you concerned that infection has started instead of healing. Families will almost always ask jokingly when they can fish again. Honestly, usually pretty quickly. Just don't put the wound under water until it's healed, and don't stand directly behind whoever is casting. And now for some take-home points. Fishhook removal is a simple and straightforward procedure where technique really matters. You have to know what type of hook is embedded in the skin. Retrograde does work for superficial or barbless hooks, but most fishhooks that I've seen have barbs because they are designed to stay in the fish. Advance and cut is probably the most broadly successful technique. String yank works if you're a YouTuber. Needle cover is really, I think, only for those scenarios where the family does not want a second hole. It's really actually hard to do. Local anesthesia is enough for most kids, so injecting with lidocaine. If you have time, LMX or EMLA helps with the poke a little bit. Routine antibiotics are not usually necessary. And if there's ocular involvement or if it's in a joint, call an ophthalmologist or an orthopedist. Honestly, this is one of those procedures that's really satisfying once you get comfortable with it. I love doing it with our residents and trainees. Families come in expecting something dramatic, and by the time they leave, they're surprised by how straightforward it was. And I guarantee that this is a story that they will tell for years and years. And if you do a good job and make it a good experience and perhaps even a lighthearted one, they are going to remember that. And yeah, you'll be part of somebody's fishing story. So I hope you did enjoy this first episode on minor procedures. I'm gonna do additional ones like these along the way because, you know, I think that they don't get a lot of love when it comes to traditional education. If you've got any ideas for future procedures or topics, please send them my way. As the kids would say, like, rate, and review. If you leave a review on your favorite podcast site, that would really help other people discover the show. I podcast because I think it's a great way to teach, and I've been doing so since 2013. And yes, you can remove a fishhook. Don't let this straightforward procedure become the one that got away. For PEM Currents: The Pediatric Emergency Medicine Podcast, this has been Brad Sobolewski. See you next time.  

Entre el 4 y 7 de diciembre se realizó en Pucón el XLV Congreso Chileno de Microbiología, organizado por Sociedad de Microbiología e Chile (Somich), donde hubo ponencias de los diversos campos de la microbiología a través de simposios especializados en microbiología general, acuícola, clínica, ambiental, industrial, alimentaria, agrícola, y veterinaria. En este congreso participó el Centro de Investigaciones Biológicas Aplicadas (CIBA) a través de su director y académico de la Universidad San Sebastián, sede Patagonia, Dr. Marcos Godoy, quien presentó un estudio genético de los últimos 16años de la Anemia Infecciosa del Salmón (ISAV). Por su parte, Diego Caro, jefe técnico de CIBA, dio a conocer un estudio sobre comunidades bacterianas en biofiltros de sistema de Recirculación en Acuicultura (RAS). En tanto, el investigador del centro, Marco Montes de Oca, expuso un trabajo relacionado con la genética de aeromonas. Acá el detalle de cada presentación en las voces de sus protagonistas. --- Send in a voice message: https://podcasters.spotify.com/pod/show/entrevistas-radio-sago/message

There Are No FakesGreat Places to Visit at Rutgers | Rutgers UniversityNorth Carolina State University Memorial Belltower - WikipediaKen Kesey - WikiquoteNeil Young's Ditch TrilogyFLOOD - Tired Eyes: An Appreciation of Neil Young's Ditch TrilogyShovels & Rope(1) Bob Marley - Roots, Rock, Reggae - YouTubeSaxapahaw, NCHoundmouthSun RoomTRAVIS SCOTTLOVETT OR LEAVE IT | Asheville, NC's Official Travel Site(19) Ziggy_Sobotka on X: “19 years ado today, #TheWire Season 3 Ep4: “Hamsterdam” premiered on HBO (10/10/04) “Look, we grind and y'all try to stop it. That's how we do. Why you got to go and fuck with the program?” -Fruit https://t.co/fsXsgUMKes” / X(19) Ziggy_Sobotka on X: “Hamsterdam made it on to Google Earth as a pharmacy. It has fourteen 5 star reviews. https://t.co/oMweOOpOnf” / XFood Safety Talk 53: Raw Milk Hamsterdam — Food Safety TalkJury Duty (TV Series 2023– ) - IMDb516. Horchata — Risky or Not?Frontiers | Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factorsSafety of sushi questioned by researcherTriortho cresyl phosphate “Ginger Jake” disaster—United States,1930s - ScienceDirectGinger Jake and the blues: a tragic song of poisoning - PubMedjake leg blues lyrics - Google SearchJake Leg: An Affliction and the Blues It Inspired : NPRAppearance: Food Safety Talk 254 — Liss is MoreThe Trick is Not Minding That It Hurts - Lee LeFever dot com(1) Lawrence of Arabia Trick is not minding that it hurts - YouTubeThe Subtle Art of Not Giving a F*ck: A Counterintuitive Approach to Living a Good Life: Manson, Mark: 9780062457714: Amazon.com: BooksTextExpander: #1 Text Replacement & Keyboard Shortcut AppSharpen the Saw: Exploring Covey's Final Habit | Art of ManlinessWake County Ensures Food Safety Takes Center Stage at NC State Fair | Wake County GovernmentRisk Factors for Foodborne Illness in Temporary Eating Establishments in North Carolina - International Association for Food ProtectionTara in a Smoothie cause of Liver Disfunction | Marler Bloghttps://www.marlerblog.com/files/2023/10/A-food-product-as-a-potential-serious-cause-of-liver-injury.pdfResearchers estimate the cost of foodborne infections in two African nations | Food Safety NewsFrontiers | Economic costs related to foodborne disease in Burkina Faso and Ethiopia in 2017Attribution of country level foodborne disease to food group and food types in three African countries: Conclusions from a structured expert judgment study - PMCDr Lucia Anelich – ICMSF | International Commission on Microbiological Specifications for Foods

Con capacidad total se realizará durante esta jornada la primera versión de Aqua Bacteria, organizado por el Centro de Investigaciones Biológicas Aplicadas (CIBA), el que se llevará a cabo a contar de las 14 horas en el hotel Cabañas del Lago, en Puerto Varas. En este seminario uno de los exponentes será el Doctor, Matías Poblete, de Veterquímica, quien expondrá sobre las "estrategias de inmunización para aeromonas salmonicida en la fase de agua dulce". Región Acuícola de Radio Sago conversó con el especialista, quien entregó detalles de su presentación. --- Send in a voice message: https://podcasters.spotify.com/pod/show/entrevistas-radio-sago/message

Episode 123: Spontaneous Bacterial Peritonitis.  Kaitlen defines spontaneous bacterial peritonitis (SBP) and also explains the diagnosis and management.  Written by Kaitlen Roy-Ross, MS4, Ross University School of Medicine. Moderated by Hector Arreaza, MD. Definition:An ascitic fluid infection with no obvious surgically treatable intra-abdominal source (bowel perforation, abscess, perforated ulcer). Commonly seen in patients with cirrhosis and ascites. Patients may have symptoms of fever, abdominal pain, abdominal tenderness, altered mental status, and hypotension.Etiology: The most common pathogens (75%) are gram-negative aerobic organisms. Klebsiellapneumoniae accounts for 50% of the cases. Gram-positive aerobic bacteria (Streptococcus pneumoniae or viridans group streptococcus) account for the remaining cases. Some report E. coli as the most common cause of SBP. Random information: in Korea, Aeromonas hydrophila is an important pathogen of SBP during the summer. Diagnosis: To diagnose SBP, a paracentesis should be performed to analyze the ascitic fluid prior to treating the patient with antibiotics.The ascitic fluid should be analyzed for the following: PMN (Polymorphonuclear cell) count: > or = to 250 cells/mm3 Aerobic and anaerobic culturesSerum ascites albumin gradient (serum albumin-ascitic albumin): this measures portal pressure.If the gradient is > 1.1 = portal HTN is present (cirrhosis, heart failure, large liver malignancy, alcoholic hepatitis, portal vein thrombosis) – SBP is likely.If the gradient is 6 mg/ suggests a gallbladder perforation. No SBP.Treatment:The treatment for spontaneous bacterial peritonitis is broad-spectrum antibiotics. Empiric treatment is indicated if a patient with ascites has any of the following:Temperature > 100 FAbdominal pain or tendernessAltered mental statusPMN in ascitic fluid > 250 (but if there is bacteria in ascitic fluid, start antibiotics stat)Alcohol-induced hepatitis*Important note: Patients on beta blockers should have them permanently discontinued prior to treatment for SBP as beta blockers are associated with worse outcomes. In one study, patients on beta blockers had a 58% increase in mortality risk compared to patients not treated with beta-blockers. Beta-blockers were also associated with higher rates of hepatorenal syndrome and longer lengths of hospital stay.1st line treatment- 3rd generation Cephalosporin Cefotaxime 2g IV Q8H (preferred) or Ceftriaxone 2 g per day2nd line treatment- Carbapenems. Usually reserved for patients with severe disease/critical illness.3rd line- Fluoroquinolones- Cipro 400 mg IV BID to patients with normal renal function. (Patients should not get this if they already receiving it prophylactically.)Duration of treatment:5 days, then re-assess the patient's PMN count:PMN 250 or greater than pre-treatment PMN count > look for a surgical source of infection.If PMN is > 250 but less than pre-treatment value, continue ABX for 48 more hours and then repeat paracentesis. Note: In general, ascitic fluid PMN count should be reduced by at least 25% after 48 hours of antibiotic therapy.Renal failure is the major cause of death in patients with SBP and develops in 30-40 % of the patients. We can decrease this risk by administering IV albumin. IV albumin should be given when the creatinine is > 1 mg/dl, the blood urea nitrogen is > 30 mg/dl, or the total bilirubin is > 4 mg/dl. Treatment with octreotide or midodrine is helpful if renal failure develops.Prevention:Antibiotic prophylaxis can be given to patients with risk factors for SBP. Some risk factors include prior history of SBP, variceal hemorrhage, or an ascites fluid protein concentration of

Finally found some good stories, so we're back! This episode: How slime molds encode and use memories built into their own bodies! Download Episode (4.6 MB, 6.7 minutes) Show notes: Microbe of the episode: Aeromonas salmoncida News item   Takeaways Despite being single-celled organisms, slime molds have fairly complex behavior, including a basic form of memory. They often grow as a network of tubes of cytoplasm branching out from one place to find and exploit new sources of food in their environment. When these tubes connect to new food, other less productive branches of its body shrink away.   As it turns out, this body form serves a role in memory also. This study determined that the slime mold's tubes undergo constant squeezing, which moves cell contents around and also shrinks them. When tubes are connecting to a food source though, they secrete a softening agent that allows the pressure to expand the tubes instead of shrinking them. These larger tubes consequently are capable of transporting more softening agent farther away to newer food sources, so the history of food discoveries is recorded in the slime mold's own body, which also influences its responses to new discoveries.   Journal Paper: Kramar M, Alim K. 2021. Encoding memory in tube diameter hierarchy of living flow network. Proc Natl Acad Sci 118. Other interesting stories: Bacteria-derived gene editing tool TALEN better than CRISPR in some cases Live microbes in oceans produce more hydrocarbons than oil seeps introduce, priming microbes to break down oil (paper)   Email questions or comments to bacteriofiles at gmail dot com. Thanks for listening! Subscribe: Apple Podcasts, Google Podcasts, Android, or RSS. Support the show at Patreon, or check out the show at Twitter or Facebook.

On this week's The Sci-Files, your hosts Chelsie and Danny interview Courtney Harrison.Courtney is a second-year master's student in the Department of Fisheries and Wildlife at the Michigan State University Aquatic Animal Health Laboratory. Her research investigates the effects infectious diseases have on lake whitefish (Coregonus clupeaformis) and their subsequent recruitment to the Great Lakes. Recently, lake whitefish commercial yields have been declining, along with abundance and growth. Similarly, there have been declines in early life stages (i.e., eggs and fry), and juvenile recruitment of lake whitefish to the Great Lakes.Few studies have assessed lake whitefish health, and these same studies discovered the presence of microbial pathogens in adult Great Lakes lake whitefish populations. Even more interesting is that the pathogens found are known to cause high mortality and can be shed in reproductive tissue or vertically transmitted from infected parent to offspring in other salmonids. Her research will determine disease status in both wild adult and juvenile lake whitefish aggregations within Lakes Superior, Michigan, and Huron. She will also assess the ability of viral hemorrhagic septicemia virus (VHSv) and three bacterial pathogens (Renibacterium salmoninarum, Carnobacterium sp., and Aeromonas salmonicida subsp. salmonicida), all which have been recovered from Great Lakes lake whitefish, to cause disease and/or mortality in juvenile lake whitefish in laboratory experimental challenges. Data generated from my study can be used in future recruitment models to address the uncertainty caused by disease-associated losses in current models.If you're interested in talking about your MSU research on the radio or nominating a student, please email Chelsie and Danny at scifiles@impact89fm.org. You can ask questions for our future episodes here. Check The Sci-Files out on Twitter, Facebook, Instagram, and YouTube!

O artigo que discutimos nesse episódio se chama “Response of Gut Microbiota to Metabolite Changes Induced by Endurance Exercise”, ou seja “Resposta da Microbiota intestinal a mudanças metabólicas induzidas por exercícios intensos”, mais especificamente uma meia-maratona e foi publicado em abril de 2018 na Frontiers in Microbiology, por um grupo chinês da Third Military Medical University. Resumidamente o que os autores fizeram foi comparar a microbiota intestinal de corredores antes e depois de correr uma meia-maratona. No Microlitros de Notícias vamos saber sobre as novidades na identificação de microrganismos pela técnica de MALDI TOF; As dificuldades de se estudar infecções nas membranas fetais com uma breve entrevista do pesquisador Dr. Dave Aronoff da Vanderbilt University; e a descoberta de resistência em Aeromonas à baixas concentrações de antibióticos. Na Filogenia da Ciência, teremos a vida e pesquisa do microbiologista Robert Koch, esse mesmo… o do Postulado de Koch.   Tópicos comentados nesse episódio Microbiota intestinal Alteração da Microbiota induzida por exercícios intensos Meia-maratona Identificação de microrganismos MALDI TOF Membranas fetais Dave Aronoff Instrumented Fetal Membrane on a Chip (IFMOC) Resistência à baixas concentrações de antibióticos Robert Koch Postulado de Koch Antraz   Referências desse episódio 2018.Zhao X, Zhang Z, Hu B, Huang W, Yuan C e Zou L. Response of Gut Microbiota to Metabolite Changes Induced by Endurance Exercise. Front Microbiol. 2018.  Vrioni G, Tsiamis C, Oikonomidis G, Theodoridou K, Kapsimali V e Tsakris A. MALDI-TOF mass spectrometry technology for detecting biomarkers of antimicrobial resistance: current achievements and future perspectives. ATM. 2009. Piseth Seng, Michel Drancourt, Frédérique Gouriet, Bernard La Scola, Pierre-Edouard Fournier, Jean Marc Rolain e Didier Raoult. Ongoing Revolution in Bacteriology: Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry. Clin Infect Dis. 2017. Gnecco JS, Anders AP, Cliffel D, Pensabene V, Rogers LM, Osteen K e Aronoff DM.  Instrumenting a Fetal Membrane on a Chip as Emerging Technology for Preterm Birth Research. Curr Pharm Des. 2010. Blevins SM, Bronze MS. Robert Koch and the 'golden age' of bacteriology. 2010. Sociedade Brasileira de Microbiologia. Robert Koch: grande descobridor de pequenas bactérias. Biblioteca Virtual em Saúde Adolpho Lutz. Robert Koch. Noel Prize.org. Robert Koch - Biographical   Sobre o Podcast Microbiando A ideia do Microbiando é discutir artigos científicos de ponta em todas as áreas da microbiologia e imunologia. Vamos utilizar uma linguagem bem acessível para destrinchar esses artigos para vocês, mas sem perder o rigor científico e analítico necessário para essa tarefa. Além de discutir artigos nós teremos o quadro Microlitros de Notícias, onde nossos microbiologistas e imunologistas de plantão irão abordar pequenas reportagens e trazer novidades para vocês. No quadro filogenia da Ciência vamos contar um pouco sobre a vida de grandes personalidades que revolucionaram a Microbiologia e Imunologia com suas descobertas. Você pode nos ouvir no Spotify, Google Podcast, Player FM, Podcast Addict, CastBox, Blubrry Podcasting, iTunes e outros agregadores de podcasts.   Contatos E-mail: microbiando@micro.ufrj.br Twitter Facebook Instagram   Expediente Produção Geral: Rosana Ferreira Hosts: Rosana Ferreira Equipe de Pauta/Gravação: Cláudia M. d'Avila-Levy, Juliana Echevarria, Leandro Lobo, Mateus Godoy, Rosana Ferreira, Sidcley Lyra, Cecília Vieira, Gabriel Martins, Gustavo Meira, Michel Leon e Úrsula Lopes. Edição: Hugo Marins/NNOTEM (Núcleo de Novas Tecnologias e Mídias/IBCCF) Trilha Sonora: Daniel Vasquez   O podcast Microbiando tem o apoio do Instituto de Microbiologia Professor Paulo de Góes e do Instituto de Biofísica...

Vincent, Michael, and Elio review necrotizing fasciitis, and a link between surface remodeling in gram-positive and gram-negative bacteria.

Extracellular proteome of Aeromonas hydrophila AH-1, Albumin and Hsp70 in corneal epithelial wound repair, Arginine-mediated protein expression in intestinal Caco-2 cells, 3D-LC-MS/MS proteomics

Extracellular proteome of Aeromonas hydrophila AH-1, Albumin and Hsp70 in corneal epithelial wound repair, Arginine-mediated protein expression in intestinal Caco-2 cells, 3D-LC-MS/MS proteomics

1. Der Aufbau des Membranpotentials und die ATP-Synthese in H. halophilus wurden durch Cl- nicht beeinflußt. Zusammen mit den Ergebnisse früherer Studien gibt es keinen Hinweis darauf, daß Cl- an der primären Bioenergetik von H. halophilus beteiligt ist. 2. Es wurde ein unter Hochsalzbedingungen induziertes, Cl--abhängiges Transportsystem für das kompatible Solut Betain identifiziert und charakterisiert. Dieses System transportiert Betain mit einer maximalen Geschwindigkeit von Vmax.= 14,0 ± 0,2 nmol/min x mg Protein und hat einen Km-Wert für Betain von 72,8 ± 10,4 µM. Die Ergebnisse der durchgeführten Hemmstoffstudien deuten darauf hin, daß die Betain-Aufnahme in H. halophilus über einen primären Transportmechanismus erfolgt. 3. Die Beweglichkeit von H. halophilus auf Weichagarplatten zeigt eine klare Cl--Abhängigkeit. In elektronenmikroskopischen Untersuchungen konnte festgestellt werden, daß die Flagellenbildung in H. halophilus Cl--abhängig ist. Das Flagellin wurde gereinigt, und es wurde ein spezifisches Antiserum dagegen hergestellt. 4. Immunologische Analysen ergaben, daß die Synthese des Flagellins Wachstumsphasen-abhängig war. In der log-Phase und in der frühen stationären Phase wurden große Mengen an Flagellin nachgewiesen, während die Flagellinkonzentration in der späten stationären Phase zurückging. Interessanterweise war die Flagellinsynthese zu jedem Zeitpunkt des Wachstums Cl--abhängig; in Abwesenheit von Cl- war kein Flagellin nachzuweisen. Dies ist der erste Nachweis einer Cl--abhängigen Proteinproduktion in einem Prokaryonten. 5. Es wurde eine Plasmid-Genbank aus chromosomaler DNA von H. halophilus generiert, die 5807 Klone mit einer durchschnittlichen Fragmentgröße von 4415 Bp enthält. Dies entspricht einer Wahrscheinlichkeit von 99,8%, daß sämtliche Bereiche des Genoms von H. halophilus abgedeckt wurden. Die für das Flagellin (fliC) und die β-Untereinheit der F1FO-ATP-Synthase (atpD) aus H. halophilus kodierenden Gene wurden mit Hilfe von Koloniehybridisierungen in der Genbank identifiziert. Anschließend wurden Teile dieser Gene kloniert und sequenziert. 6. Northern-Blot- und RT-PCR-Analysen zeigten, daß die Transkription von fliC durch Cl- um den Faktor 2 stimuliert wird. Dies ist der erste Nachweis einer durch Cl- stimulierten Transkription eines Gens mit bekannter Funktion. 7. Versuche zur Substitution von Cl- durch kompatible Solute ergaben, daß Glutamat, Succinat und Fumarat die Cl--Abhängigkeit des Wachstums von H. halophilus aufheben können. Für Glutamat wurde gezeigt, daß dies auf die nicht Cl--abhängige Aufnahme von Glutamat zurückzuführen ist. Für die Beweglichkeit und die Flagellinsynthese wurde gezeigt, daß Glutamat Cl- nicht effektiv substituieren kann. 8. Mit Hilfe von 2D-gelelektrophoretischen Studien konnten 5 weitere Cl-- abhängig synthetisierte Proteine in H. halophilus nachgewiesen werden. Die Identifizierung dieser Proteine erfolgte durch N-terminale Sequenzierung und nachfolgender Suche nach ähnlichen Proteinen in Datenbanken. Zwei davon, YvyD und SodA, gehören zum σB-Regulon von B. subtilis. YvyD ist von besonderem Interesse, da es als σ-Faktor modulierendes Protein an der Cl-- abhängigen Signaltransduktionskette, die von der Wahrnehmung des Reizes zur Genexpression führt, beteiligt sein könnte. Ein drittes Protein (YhfK) ist Aspartatund Glutamat-Semialdehyd-Dehydrogenasen sehr ähnlich. Das vierte Protein ist der ATP-bindenden Untereinheit verschiedener ABC-Transporter sehr ähnlich. Das fünfte identifizierte Protein, LuxS, ist in Gram-negativen an der Biosynthese von Autoinduktoren beteiligt. 9. Teile der Gene, die in H. halophilus für YvyD bzw. LuxS kodieren, wurden mit Hilfe von degenerierten Oligonukleotiden per PCR amplifiziert, kloniert und sequenziert. 10. In Wachstumsversuchen konnte gezeigt werden, daß 11 von 44 darauf untersuchten Arten Gram-positiver und Gram-negativer Bakterien eine Cl-- abhängige Osmotoleranz aufweisen. Dies waren: Aeromonas hydrophila, Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Paracoccus denitrificans, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Thermus thermophilus und Vibrio fischeri.