Podcasts about aeromonas

Entre el 4 y 7 de diciembre se realizó en Pucón el XLV Congreso Chileno de Microbiología, organizado por Sociedad de Microbiología e Chile (Somich), donde hubo ponencias de los diversos campos de la microbiología a través de simposios especializados en microbiología general, acuícola, clínica, ambiental, industrial, alimentaria, agrícola, y veterinaria. En este congreso participó el Centro de Investigaciones Biológicas Aplicadas (CIBA) a través de su director y académico de la Universidad San Sebastián, sede Patagonia, Dr. Marcos Godoy, quien presentó un estudio genético de los últimos 16años de la Anemia Infecciosa del Salmón (ISAV). Por su parte, Diego Caro, jefe técnico de CIBA, dio a conocer un estudio sobre comunidades bacterianas en biofiltros de sistema de Recirculación en Acuicultura (RAS). En tanto, el investigador del centro, Marco Montes de Oca, expuso un trabajo relacionado con la genética de aeromonas. Acá el detalle de cada presentación en las voces de sus protagonistas. --- Send in a voice message: https://podcasters.spotify.com/pod/show/entrevistas-radio-sago/message

There Are No FakesGreat Places to Visit at Rutgers | Rutgers UniversityNorth Carolina State University Memorial Belltower - WikipediaKen Kesey - WikiquoteNeil Young's Ditch TrilogyFLOOD - Tired Eyes: An Appreciation of Neil Young's Ditch TrilogyShovels & Rope(1) Bob Marley - Roots, Rock, Reggae - YouTubeSaxapahaw, NCHoundmouthSun RoomTRAVIS SCOTTLOVETT OR LEAVE IT | Asheville, NC's Official Travel Site(19) Ziggy_Sobotka on X: “19 years ado today, #TheWire Season 3 Ep4: “Hamsterdam” premiered on HBO (10/10/04) “Look, we grind and y'all try to stop it. That's how we do. Why you got to go and fuck with the program?” -Fruit https://t.co/fsXsgUMKes” / X(19) Ziggy_Sobotka on X: “Hamsterdam made it on to Google Earth as a pharmacy. It has fourteen 5 star reviews. https://t.co/oMweOOpOnf” / XFood Safety Talk 53: Raw Milk Hamsterdam — Food Safety TalkJury Duty (TV Series 2023– ) - IMDb516. Horchata — Risky or Not?Frontiers | Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factorsSafety of sushi questioned by researcherTriortho cresyl phosphate “Ginger Jake” disaster—United States,1930s - ScienceDirectGinger Jake and the blues: a tragic song of poisoning - PubMedjake leg blues lyrics - Google SearchJake Leg: An Affliction and the Blues It Inspired : NPRAppearance: Food Safety Talk 254 — Liss is MoreThe Trick is Not Minding That It Hurts - Lee LeFever dot com(1) Lawrence of Arabia Trick is not minding that it hurts - YouTubeThe Subtle Art of Not Giving a F*ck: A Counterintuitive Approach to Living a Good Life: Manson, Mark: 9780062457714: Amazon.com: BooksTextExpander: #1 Text Replacement & Keyboard Shortcut AppSharpen the Saw: Exploring Covey's Final Habit | Art of ManlinessWake County Ensures Food Safety Takes Center Stage at NC State Fair | Wake County GovernmentRisk Factors for Foodborne Illness in Temporary Eating Establishments in North Carolina - International Association for Food ProtectionTara in a Smoothie cause of Liver Disfunction | Marler Bloghttps://www.marlerblog.com/files/2023/10/A-food-product-as-a-potential-serious-cause-of-liver-injury.pdfResearchers estimate the cost of foodborne infections in two African nations | Food Safety NewsFrontiers | Economic costs related to foodborne disease in Burkina Faso and Ethiopia in 2017Attribution of country level foodborne disease to food group and food types in three African countries: Conclusions from a structured expert judgment study - PMCDr Lucia Anelich – ICMSF | International Commission on Microbiological Specifications for Foods

Con capacidad total se realizará durante esta jornada la primera versión de Aqua Bacteria, organizado por el Centro de Investigaciones Biológicas Aplicadas (CIBA), el que se llevará a cabo a contar de las 14 horas en el hotel Cabañas del Lago, en Puerto Varas. En este seminario uno de los exponentes será el Doctor, Matías Poblete, de Veterquímica, quien expondrá sobre las "estrategias de inmunización para aeromonas salmonicida en la fase de agua dulce". Región Acuícola de Radio Sago conversó con el especialista, quien entregó detalles de su presentación. --- Send in a voice message: https://podcasters.spotify.com/pod/show/entrevistas-radio-sago/message

Episode 123: Spontaneous Bacterial Peritonitis.  Kaitlen defines spontaneous bacterial peritonitis (SBP) and also explains the diagnosis and management.  Written by Kaitlen Roy-Ross, MS4, Ross University School of Medicine. Moderated by Hector Arreaza, MD. Definition:An ascitic fluid infection with no obvious surgically treatable intra-abdominal source (bowel perforation, abscess, perforated ulcer). Commonly seen in patients with cirrhosis and ascites. Patients may have symptoms of fever, abdominal pain, abdominal tenderness, altered mental status, and hypotension.Etiology: The most common pathogens (75%) are gram-negative aerobic organisms. Klebsiellapneumoniae accounts for 50% of the cases. Gram-positive aerobic bacteria (Streptococcus pneumoniae or viridans group streptococcus) account for the remaining cases. Some report E. coli as the most common cause of SBP. Random information: in Korea, Aeromonas hydrophila is an important pathogen of SBP during the summer. Diagnosis: To diagnose SBP, a paracentesis should be performed to analyze the ascitic fluid prior to treating the patient with antibiotics.The ascitic fluid should be analyzed for the following: PMN (Polymorphonuclear cell) count: > or = to 250 cells/mm3 Aerobic and anaerobic culturesSerum ascites albumin gradient (serum albumin-ascitic albumin): this measures portal pressure.If the gradient is > 1.1 = portal HTN is present (cirrhosis, heart failure, large liver malignancy, alcoholic hepatitis, portal vein thrombosis) – SBP is likely.If the gradient is 6 mg/ suggests a gallbladder perforation. No SBP.Treatment:The treatment for spontaneous bacterial peritonitis is broad-spectrum antibiotics. Empiric treatment is indicated if a patient with ascites has any of the following:Temperature > 100 FAbdominal pain or tendernessAltered mental statusPMN in ascitic fluid > 250 (but if there is bacteria in ascitic fluid, start antibiotics stat)Alcohol-induced hepatitis*Important note: Patients on beta blockers should have them permanently discontinued prior to treatment for SBP as beta blockers are associated with worse outcomes. In one study, patients on beta blockers had a 58% increase in mortality risk compared to patients not treated with beta-blockers. Beta-blockers were also associated with higher rates of hepatorenal syndrome and longer lengths of hospital stay.1st line treatment- 3rd generation Cephalosporin Cefotaxime 2g IV Q8H (preferred) or Ceftriaxone 2 g per day2nd line treatment- Carbapenems. Usually reserved for patients with severe disease/critical illness.3rd line- Fluoroquinolones- Cipro 400 mg IV BID to patients with normal renal function. (Patients should not get this if they already receiving it prophylactically.)Duration of treatment:5 days, then re-assess the patient's PMN count:PMN 250 or greater than pre-treatment PMN count > look for a surgical source of infection.If PMN is > 250 but less than pre-treatment value, continue ABX for 48 more hours and then repeat paracentesis. Note: In general, ascitic fluid PMN count should be reduced by at least 25% after 48 hours of antibiotic therapy.Renal failure is the major cause of death in patients with SBP and develops in 30-40 % of the patients. We can decrease this risk by administering IV albumin. IV albumin should be given when the creatinine is > 1 mg/dl, the blood urea nitrogen is > 30 mg/dl, or the total bilirubin is > 4 mg/dl. Treatment with octreotide or midodrine is helpful if renal failure develops.Prevention:Antibiotic prophylaxis can be given to patients with risk factors for SBP. Some risk factors include prior history of SBP, variceal hemorrhage, or an ascites fluid protein concentration of

Finally found some good stories, so we're back! This episode: How slime molds encode and use memories built into their own bodies! Download Episode (4.6 MB, 6.7 minutes) Show notes: Microbe of the episode: Aeromonas salmoncida News item   Takeaways Despite being single-celled organisms, slime molds have fairly complex behavior, including a basic form of memory. They often grow as a network of tubes of cytoplasm branching out from one place to find and exploit new sources of food in their environment. When these tubes connect to new food, other less productive branches of its body shrink away.   As it turns out, this body form serves a role in memory also. This study determined that the slime mold's tubes undergo constant squeezing, which moves cell contents around and also shrinks them. When tubes are connecting to a food source though, they secrete a softening agent that allows the pressure to expand the tubes instead of shrinking them. These larger tubes consequently are capable of transporting more softening agent farther away to newer food sources, so the history of food discoveries is recorded in the slime mold's own body, which also influences its responses to new discoveries.   Journal Paper: Kramar M, Alim K. 2021. Encoding memory in tube diameter hierarchy of living flow network. Proc Natl Acad Sci 118. Other interesting stories: Bacteria-derived gene editing tool TALEN better than CRISPR in some cases Live microbes in oceans produce more hydrocarbons than oil seeps introduce, priming microbes to break down oil (paper)   Email questions or comments to bacteriofiles at gmail dot com. Thanks for listening! Subscribe: Apple Podcasts, Google Podcasts, Android, or RSS. Support the show at Patreon, or check out the show at Twitter or Facebook.

On this week's The Sci-Files, your hosts Chelsie and Danny interview Courtney Harrison.Courtney is a second-year master's student in the Department of Fisheries and Wildlife at the Michigan State University Aquatic Animal Health Laboratory. Her research investigates the effects infectious diseases have on lake whitefish (Coregonus clupeaformis) and their subsequent recruitment to the Great Lakes. Recently, lake whitefish commercial yields have been declining, along with abundance and growth. Similarly, there have been declines in early life stages (i.e., eggs and fry), and juvenile recruitment of lake whitefish to the Great Lakes.Few studies have assessed lake whitefish health, and these same studies discovered the presence of microbial pathogens in adult Great Lakes lake whitefish populations. Even more interesting is that the pathogens found are known to cause high mortality and can be shed in reproductive tissue or vertically transmitted from infected parent to offspring in other salmonids. Her research will determine disease status in both wild adult and juvenile lake whitefish aggregations within Lakes Superior, Michigan, and Huron. She will also assess the ability of viral hemorrhagic septicemia virus (VHSv) and three bacterial pathogens (Renibacterium salmoninarum, Carnobacterium sp., and Aeromonas salmonicida subsp. salmonicida), all which have been recovered from Great Lakes lake whitefish, to cause disease and/or mortality in juvenile lake whitefish in laboratory experimental challenges. Data generated from my study can be used in future recruitment models to address the uncertainty caused by disease-associated losses in current models.If you're interested in talking about your MSU research on the radio or nominating a student, please email Chelsie and Danny at scifiles@impact89fm.org. You can ask questions for our future episodes here. Check The Sci-Files out on Twitter, Facebook, Instagram, and YouTube!

O artigo que discutimos nesse episódio se chama “Response of Gut Microbiota to Metabolite Changes Induced by Endurance Exercise”, ou seja “Resposta da Microbiota intestinal a mudanças metabólicas induzidas por exercícios intensos”, mais especificamente uma meia-maratona e foi publicado em abril de 2018 na Frontiers in Microbiology, por um grupo chinês da Third Military Medical University. Resumidamente o que os autores fizeram foi comparar a microbiota intestinal de corredores antes e depois de correr uma meia-maratona. No Microlitros de Notícias vamos saber sobre as novidades na identificação de microrganismos pela técnica de MALDI TOF; As dificuldades de se estudar infecções nas membranas fetais com uma breve entrevista do pesquisador Dr. Dave Aronoff da Vanderbilt University; e a descoberta de resistência em Aeromonas à baixas concentrações de antibióticos. Na Filogenia da Ciência, teremos a vida e pesquisa do microbiologista Robert Koch, esse mesmo… o do Postulado de Koch.   Tópicos comentados nesse episódio Microbiota intestinal Alteração da Microbiota induzida por exercícios intensos Meia-maratona Identificação de microrganismos MALDI TOF Membranas fetais Dave Aronoff Instrumented Fetal Membrane on a Chip (IFMOC) Resistência à baixas concentrações de antibióticos Robert Koch Postulado de Koch Antraz   Referências desse episódio 2018.Zhao X, Zhang Z, Hu B, Huang W, Yuan C e Zou L. Response of Gut Microbiota to Metabolite Changes Induced by Endurance Exercise. Front Microbiol. 2018.  Vrioni G, Tsiamis C, Oikonomidis G, Theodoridou K, Kapsimali V e Tsakris A. MALDI-TOF mass spectrometry technology for detecting biomarkers of antimicrobial resistance: current achievements and future perspectives. ATM. 2009. Piseth Seng, Michel Drancourt, Frédérique Gouriet, Bernard La Scola, Pierre-Edouard Fournier, Jean Marc Rolain e Didier Raoult. Ongoing Revolution in Bacteriology: Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry. Clin Infect Dis. 2017. Gnecco JS, Anders AP, Cliffel D, Pensabene V, Rogers LM, Osteen K e Aronoff DM.  Instrumenting a Fetal Membrane on a Chip as Emerging Technology for Preterm Birth Research. Curr Pharm Des. 2010. Blevins SM, Bronze MS. Robert Koch and the 'golden age' of bacteriology. 2010. Sociedade Brasileira de Microbiologia. Robert Koch: grande descobridor de pequenas bactérias. Biblioteca Virtual em Saúde Adolpho Lutz. Robert Koch. Noel Prize.org. Robert Koch - Biographical   Sobre o Podcast Microbiando A ideia do Microbiando é discutir artigos científicos de ponta em todas as áreas da microbiologia e imunologia. Vamos utilizar uma linguagem bem acessível para destrinchar esses artigos para vocês, mas sem perder o rigor científico e analítico necessário para essa tarefa. Além de discutir artigos nós teremos o quadro Microlitros de Notícias, onde nossos microbiologistas e imunologistas de plantão irão abordar pequenas reportagens e trazer novidades para vocês. No quadro filogenia da Ciência vamos contar um pouco sobre a vida de grandes personalidades que revolucionaram a Microbiologia e Imunologia com suas descobertas. Você pode nos ouvir no Spotify, Google Podcast, Player FM, Podcast Addict, CastBox, Blubrry Podcasting, iTunes e outros agregadores de podcasts.   Contatos E-mail: microbiando@micro.ufrj.br Twitter Facebook Instagram   Expediente Produção Geral: Rosana Ferreira Hosts: Rosana Ferreira Equipe de Pauta/Gravação: Cláudia M. d'Avila-Levy, Juliana Echevarria, Leandro Lobo, Mateus Godoy, Rosana Ferreira, Sidcley Lyra, Cecília Vieira, Gabriel Martins, Gustavo Meira, Michel Leon e Úrsula Lopes. Edição: Hugo Marins/NNOTEM (Núcleo de Novas Tecnologias e Mídias/IBCCF) Trilha Sonora: Daniel Vasquez   O podcast Microbiando tem o apoio do Instituto de Microbiologia Professor Paulo de Góes e do Instituto de Biofísica...

Vincent, Michael, and Elio review necrotizing fasciitis, and a link between surface remodeling in gram-positive and gram-negative bacteria.

Extracellular proteome of Aeromonas hydrophila AH-1, Albumin and Hsp70 in corneal epithelial wound repair, Arginine-mediated protein expression in intestinal Caco-2 cells, 3D-LC-MS/MS proteomics

Extracellular proteome of Aeromonas hydrophila AH-1, Albumin and Hsp70 in corneal epithelial wound repair, Arginine-mediated protein expression in intestinal Caco-2 cells, 3D-LC-MS/MS proteomics

1. Der Aufbau des Membranpotentials und die ATP-Synthese in H. halophilus wurden durch Cl- nicht beeinflußt. Zusammen mit den Ergebnisse früherer Studien gibt es keinen Hinweis darauf, daß Cl- an der primären Bioenergetik von H. halophilus beteiligt ist. 2. Es wurde ein unter Hochsalzbedingungen induziertes, Cl--abhängiges Transportsystem für das kompatible Solut Betain identifiziert und charakterisiert. Dieses System transportiert Betain mit einer maximalen Geschwindigkeit von Vmax.= 14,0 ± 0,2 nmol/min x mg Protein und hat einen Km-Wert für Betain von 72,8 ± 10,4 µM. Die Ergebnisse der durchgeführten Hemmstoffstudien deuten darauf hin, daß die Betain-Aufnahme in H. halophilus über einen primären Transportmechanismus erfolgt. 3. Die Beweglichkeit von H. halophilus auf Weichagarplatten zeigt eine klare Cl--Abhängigkeit. In elektronenmikroskopischen Untersuchungen konnte festgestellt werden, daß die Flagellenbildung in H. halophilus Cl--abhängig ist. Das Flagellin wurde gereinigt, und es wurde ein spezifisches Antiserum dagegen hergestellt. 4. Immunologische Analysen ergaben, daß die Synthese des Flagellins Wachstumsphasen-abhängig war. In der log-Phase und in der frühen stationären Phase wurden große Mengen an Flagellin nachgewiesen, während die Flagellinkonzentration in der späten stationären Phase zurückging. Interessanterweise war die Flagellinsynthese zu jedem Zeitpunkt des Wachstums Cl--abhängig; in Abwesenheit von Cl- war kein Flagellin nachzuweisen. Dies ist der erste Nachweis einer Cl--abhängigen Proteinproduktion in einem Prokaryonten. 5. Es wurde eine Plasmid-Genbank aus chromosomaler DNA von H. halophilus generiert, die 5807 Klone mit einer durchschnittlichen Fragmentgröße von 4415 Bp enthält. Dies entspricht einer Wahrscheinlichkeit von 99,8%, daß sämtliche Bereiche des Genoms von H. halophilus abgedeckt wurden. Die für das Flagellin (fliC) und die β-Untereinheit der F1FO-ATP-Synthase (atpD) aus H. halophilus kodierenden Gene wurden mit Hilfe von Koloniehybridisierungen in der Genbank identifiziert. Anschließend wurden Teile dieser Gene kloniert und sequenziert. 6. Northern-Blot- und RT-PCR-Analysen zeigten, daß die Transkription von fliC durch Cl- um den Faktor 2 stimuliert wird. Dies ist der erste Nachweis einer durch Cl- stimulierten Transkription eines Gens mit bekannter Funktion. 7. Versuche zur Substitution von Cl- durch kompatible Solute ergaben, daß Glutamat, Succinat und Fumarat die Cl--Abhängigkeit des Wachstums von H. halophilus aufheben können. Für Glutamat wurde gezeigt, daß dies auf die nicht Cl--abhängige Aufnahme von Glutamat zurückzuführen ist. Für die Beweglichkeit und die Flagellinsynthese wurde gezeigt, daß Glutamat Cl- nicht effektiv substituieren kann. 8. Mit Hilfe von 2D-gelelektrophoretischen Studien konnten 5 weitere Cl-- abhängig synthetisierte Proteine in H. halophilus nachgewiesen werden. Die Identifizierung dieser Proteine erfolgte durch N-terminale Sequenzierung und nachfolgender Suche nach ähnlichen Proteinen in Datenbanken. Zwei davon, YvyD und SodA, gehören zum σB-Regulon von B. subtilis. YvyD ist von besonderem Interesse, da es als σ-Faktor modulierendes Protein an der Cl-- abhängigen Signaltransduktionskette, die von der Wahrnehmung des Reizes zur Genexpression führt, beteiligt sein könnte. Ein drittes Protein (YhfK) ist Aspartatund Glutamat-Semialdehyd-Dehydrogenasen sehr ähnlich. Das vierte Protein ist der ATP-bindenden Untereinheit verschiedener ABC-Transporter sehr ähnlich. Das fünfte identifizierte Protein, LuxS, ist in Gram-negativen an der Biosynthese von Autoinduktoren beteiligt. 9. Teile der Gene, die in H. halophilus für YvyD bzw. LuxS kodieren, wurden mit Hilfe von degenerierten Oligonukleotiden per PCR amplifiziert, kloniert und sequenziert. 10. In Wachstumsversuchen konnte gezeigt werden, daß 11 von 44 darauf untersuchten Arten Gram-positiver und Gram-negativer Bakterien eine Cl-- abhängige Osmotoleranz aufweisen. Dies waren: Aeromonas hydrophila, Bacillus megaterium, Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Paracoccus denitrificans, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Thermus thermophilus und Vibrio fischeri.