Podcasts about ctls

This episode, hosted by Alex Usher, features a discussion with Mary C. Wright from Brown University about Centers for Teaching and Learning (CTLs) in higher education. Wright, the author of a recent book on CTLs, shares insights from her research on the evolving aims and strategies of these centers across U.S. campuses. The conversation explores CTLs as change agents, advocating for inclusive learning environments despite structural challenges. Wright introduces the HITS framework, categorizing CTLs based on their change strategies—Hubs, Incubators, Temples, and Sieves—and discusses their role in policy implementation and leadership. 

Last November, Caterpillar launched its 255 and 265 compact track loaders (CTLs). They were also on exhibit during 2024 World of Concrete, where we were thrilled to talk with Kevin Coleman, senior product specialist for Caterpillar, about them. In this podcast, we discuss the versatility, cab comfort and why Kevin calls them the “more machines:” because they have more power; more lift; more legroom; more comfort; and much more. Listen to the podcast to learn more.

Josh Eyler, acclaimed author of How Humans Learn, delves into the pivotal role of storytelling in teaching, learning, and educational development and some of the systemic issues around grading practices tackled in his upcoming book, Scarlet Letters. He offers reflections and stories about his own developmental journey leading different CTLs and shares insights and inspiration for others engaged in educational development and institutional change. Josh Eyler is Director of the Center for Excellence in Teaching & Learning and Director of the Think Forward Quality Enhancement Plan at the University of Mississippi, where he is also Clinical Assistant Professor of Teacher Education. He previously worked on teaching and learning initiatives at Columbus State University, George Mason University, and Rice University. A sought-after speaker for his expertise about the science of learning and about compassion in education, especially in connection with students, grades, and mental health, he has spoken at college and universities across the country. Eyler is the author of the book How Humans Learn: The Science and Stories behind Effective College Teaching (West Virginia University Press, 2018), which Book Authority named one of the “100 Best Education Books of All Time”. Called a “splendid repository of ways to rethink how we teach college” by the Los Angeles Review of Books, it was named a “Book of the Year” in the Chicago Tribune. His second book is forthcoming from Johns Hopkins UP in 2024. Scarlet Letters: How Grades are Harming Children and Young Adults, and What We Can Do about It, is about one of the most urgent issues in education today, grading and alternative assessment. Transcript

Have Centers for Teaching and Learning (CTLs) actually created change in higher education teaching? Have they been able to demonstrate this change? How have their strategies evolved and how are they connecting with institutional priorities for larger scale changes? Today we speak with Mary Wright, Associate Provost for Teaching and Learning at Brown University and author of the newly released book, Centers for Teaching and Learning: The New Landscape of Higher Education (2023), for which she surveyed over 1,200 CTLs in universities across the U.S. In this episode, Dr. Wright helps answer these questions and dispels other dead ideas about CTLs. ResourceCenters for Teaching and Learning: The New Landscape of Higher Education (2023) by Mary Wright, published through JHUPress. Use promo code HCTL23 in the check-out for a discount (active through 7/7/24). 

MDJ Script/ Top Stories for July28th Friday Publish Date: July 27 Thursday   Commercial: Henssler :15   From the Henssler Financial Studio, Welcome to the Marietta Daily Journal Podcast Today is Friday July 28th and happy 75th birthday to legendary NBC producer Dick Ebersol ***Ebersol*** I'm Dan Radcliffe and here are the stories Cobb is talking about, presented by Engineered Solutions of Georgia 1. 1 dead, 2 hospitalized in Cobb County house fire 2. Acworth police traffic stop leads to arrest of man charged with multiple felonies 3. And the Cobb commission hikes water fees over GOP opposition   All of this and more is coming up on the Marietta Daily Journal Podcast. Commercial : ESOG   STORY 1: fire   One person died, and two others were hospitalized due to a house fire on Merry Oak Road, off Hurt Road, according to Cobb Fire. The victim was identified as Nancy J. Martin, 75. The fire broke out around 5:30 a.m., and two residents, a woman and her child, managed to escape through a window before firefighters arrived. Firefighters rescued the three trapped individuals, initiating resuscitative efforts. The woman who escaped was injured but conscious, and the child was unharmed. The house was completely destroyed by the fire. The cause of the fire remains unknown and is being investigated. A family friend, Cole Long, expressed deep sorrow, describing the victim as a mother figure to him.....................……... Read more about this at mdjonline.com   Story 2: traffic   A routine traffic stop in Acworth led to a chase and the arrest of Youssef Amer, a 25-year-old man from Kennesaw. The stop was initially for lacking insurance and registration on his vehicle, but police discovered that Amer was wanted for several serious offenses, including aggravated assault and a probation violation related to armed robbery and aggravated battery. After setting up a perimeter and conducting an extensive search, officers, with assistance from other agencies, successfully located and apprehended Amer. During the arrest, they also found a large quantity of marijuana and THC oil in his possession. Amer is now facing multiple charges and is being held without bond at the Cobb jail.   Story 3: water   Cobb County residents will experience a 7.5% rise in their water bills from October 1, the second increase in two years. The Cobb Board of Commissioners approved the hike with a 3-2 vote, with two Republicans opposing. Commissioner JoAnn Birrell is against rate increases while the county transfers a portion of the water system's revenues to the general fund. The transfer percentage dropped from 7% to 6% in the recent budget. The chief financial officer, Bill Volckmann, stated that the reduction would put $2.5 million back into the water system. The water transfer is legal, but some commissioners are concerned about its impact on water rates. The increase is necessary due to higher costs in chemicals, biosolids disposal, wholesale water, materials, and the need for infrastructure replacements and upgrades. For the average residential water and sewer customer, the monthly increase will be $5.45.   we'll be right back   Break: CU of GA – Elon – Dayco   STORY 4: shuttle   The Cumberland Community Improvement District (CID) has introduced a new self-driving shuttle called the "Cumberland Hopper" that operates on two routes. The shuttle, manufactured by Navya and operated by Beep, can seat eight passengers and is part of an eight-month pilot program to collect data on moving people through the busy commercial district via self-driving vehicles. The routes include a back-and-forth run on a pedestrian bridge connecting Cobb Galleria to The Battery Atlanta and a loop around the Galleria office park, stopping at various locations. The ultimate goal is to have a fleet of shuttles connecting major destinations in Cumberland, offering alternative transportation options for residents and visitors. The shuttle is monitored remotely and operates on a virtual rail system. Attendees praised the shuttle's comfort and potential to enhance mobility options in the area.   STORY 5: divided   The Cobb Board of Commissioners approved a $1.2 billion fiscal year 2024 budget, representing a 3.8% increase from the previous year. The budget includes 34 new staff positions and raises of 3-4% for some employees. It allocates funds for various initiatives, such as a micro transit program, emergency warming shelters, a family advocacy center, and a countywide grant writer. The budget also adds $1 million to improve the county's permitting process for businesses. The county's general fund will increase by 3.4%, while the fire fund will grow by 13.6%. The budget passed in a party-line vote, with two GOP commissioners opposing due to concerns about the millage rate and spending.   Story 6: teachers   Around 600 new teachers recently attended the Cobb Schools New Teacher Academy, with some coming from neighboring school districts and even other states due to Cobb's reputation as one of the best places to teach. The district's focus on employees and programs like Georgia's BEST, which offers tuition-free advanced degrees, attracted many veteran educators. Superintendent Chris Ragsdale highlighted the district's commitment to student success, safety, and the use of the Cobb Teaching and Learning System (CTLS). During the academy, educators learned about CTLS and engaged in content-specific sessions to prepare for the upcoming school year. Building relationships with students was emphasized, and the new teachers were encouraged to make a positive difference every day.   We'll be back in a moment   Break: Drake- Ingles 6- G.O.N.   Story 7: Pigs   The 22nd Annual Pigs & Peaches BBQ Festival, presented by Williams Elleby Law Firm, will take place on August 18th from 6 to 10 p.m. and August 19th from 10 a.m. to 10 p.m. at Adams Park in Kennesaw. The festival, now in its 22nd year, offers two days of music, food, beer, and family-friendly activities, attracting around 65,000 attendees annually. The event includes over 100 vendor booths, a Kid Zone with inflatables and amusements, and a Kansas City Barbeque Society sanctioned competition with cash and prizes awarded to winners. Headlining acts include Drake Milligan and Allie Colleen. The festival concludes on August 19th with a fireworks finale at 9:30 p.m. VIP Experience tickets are available for purchase and include various amenities. Other activities like the Pigs & Peaches Cornhole Classic and Parents Night Out are also scheduled. More information can be found on the festival's website or by contacting Kennesaw Parks & Recreation.   Story 8: Clemmons   Chase Clemmons, a former Etowah point guard, hosted a back-to-school event at the Salvation Army's Marietta facility before starting his basketball journey with Kennesaw State's team. He distributed free bookbags, school supplies, and other essentials to help families in need. Clemmons, who values giving back to the community, expressed his desire to make a positive impact as a student-athlete. The event, sponsored by Jersey Mike's and TEN91 Sports, was a success, with over 50 bookbags given out to 70 kids. After leading Etowah to the Region 6-6A championship, Clemmons now joins Kennesaw State's basketball program, aiming to make a strong first impression.   Story 9: AVP   The AVP Pro Beach Volleyball Tour's Gold Series Atlanta Open will take place at Atlantic Station from August 4th to 6th. The event will feature top professional beach volleyball athletes competing for men's and women's titles, including night sessions under the lights. The tournament has a rich history in Atlanta, and past champions include renowned names like Misty May-Treanor and Karch Kiraly. Notable athletes expected to compete include Taryn Kloth and Kristen Nuss, who made history by winning last year's Atlanta Open in their professional debut. The event will be streamed live on ESPN+ and TSN+ in Canada. Tickets are available for purchase on the AVP website.     We're back with final thoughts after this     Break: UMC 15 - Glover Park. - Henssler 60 Signoff- Thanks again for hanging out with us on today's Marietta Daily Journal podcast. Read more about all our stories, and get other great content at MDJonline.com. Did you know over 50% of Americans listen to podcasts weekly? Giving you important news about our community and telling great stories are what we do. Make sure you join us for our next episode and be sure to share this podcast on social media with your friends and family. Add us to your Alexa Flash Briefing or your Google Home Briefing and be sure to like, follow, and subscribe wherever you get your podcasts. www.cuofga.org  www.esogrepair.com  www.daycosystems.com  www.drakerealty.com  www.gon.com  www.ingles-markets.com  www.henssler.com  www.unitedmilitarycare.org  www.elonsalon.com  See omnystudio.com/listener for privacy information.

This episode is about mutations especially types of mutations and their effects on proteins, genetic variation and viral antigenic drift and also answers some great questions asked by listeners about a range of things that often confuse students, such as why we are not still immune to a virus that has undergone antigenic shift, or why we have both NK cells and Tc cells (CTLs) when they both seem to do the same thing ... and more. Ask your questions for discussion on the upcoming podcast: - Email to biologypodcast@gmail.com © 2022 Andrew Douch This work is copyright. Apart from any use permitted under the Copyright Act 1968, no part may be reproduced by any process without prior permission from Andrew Douch. Requests and enquiries concerning reproduction rights should be made in writing at: biologypodcast@gmail.com Disclaimers: 1. The explanations provided in this podcast are given in good faith but no responsibility will be taken for their accuracy. 2. The opinions expressed in this podcast are my own. They do not represent the opinions of the VCAA or any other organisation or government body. 3. No guarantee is made that the podcast makes a thorough coverage of all aspects of the course, or that all things contained in the course are relevant to VCE Biology Units 3 and 4. --- Send in a voice message: https://anchor.fm/biologypodcast/message

John Deere is a name widely known in agriculture and construction. For landscapers, machines that are commonly turned to for a variety of tasks in excavation, loading, trenching and more, are compact track loaders and skid steers. On this episode of the Grass Roots podcast, Green Industry Pros Editor Ryan Whisner talks with John Deere Product Marketing Manager Gregg Zupancic about some of the benefits and applications for the CTLS and skid steers as it relates to landscaping.

In this episode of Connected to the Land Podcast with 4Rivers Equipment, Fred Eichler, and Neil Motley, Branch Manager at 4Rivers Equipment in Strasburg, CO talks through skid steers, compact track loaders (CTLs), and compact wheel loaders. These versatile machines prove to be some of the handiest equipment at 4Rivers Equipment. Learn about floatation and traction, dozer attachments, bale accumulators, and more! This one will get you thinking about all the different use cases for these compact machines! 4Rivers Sponsored Technician Program: https://www.4riversequipment.com/company/careers/service-career-4rivers-equipment/techsponsorship/

Eric Zieser, Case Construction Equipment product manager, talks about three of the driving forces behind the redesign of the Case B Series skid steers and compact track loaders. The new machines honed in on operator comfort, vehicle performance, and durability and reliability of the machine. One of the biggest improvements is Case has integrated the operational systems with new control and operator interfaces so customers can better utilize the units. An 8-in. LCD display provides the operators better control of the machine. Plus, a standard backup camera offers better visibility to the rear of the machine. According to Zieser, Case wanted to leverage the technology that was already available in the units and make it easier and more intuitive for operators to use.

Eine kritische Vorraussetzung für eine effektive Krebstherapie stellt die Identifizierung von Tumor-spezifischen oder Tumor-assoziierten Antigenen (TAAs) dar. Diese Antigene sollten Peptidsequenzen besitzen, die an MHC-Moleküle binden. Auch sollten diese von Tumorzellen prozessiert und auf MHC-Molekülen präsentiert werden. Ein weiteres TAA-Kriterium stellt die Überexpression im Tumor dar, was eine Erkennung durch T-Zellen ermöglicht und folglich eine Tumor-spezifische Immunantwort nach sich ziehen soll. Bei der B-chronischen lymphatischen Leukämie (B-CLL) wurden bislang nur wenige Tumorantigene identifiziert, die als potentielle Zielstrukturen für eine Generierung einer spezifischen T-Zellantwort in Frage kommen. Daher sind die Bestrebungen groß, neue B CLL-assoziierte Antigene zu identifizieren. In dieser Arbeit wurden die Moleküle hTERT, CD23 und CD229 hinsichtlich ihrer Möglichkeiten untersucht, als TAAs bei der B-CLL zu fungieren. Die katalytische Untereinheit der humanen Telomerase Reverse Transkripase (hTERT), stellt ein universelles Tumorantigen dar, das in einer Vielzahl verschiedener Krebstypen, einschließlich hämatopoetischer Erkrankungen, exprimiert wird, jedoch nicht oder nur in geringem Maße bei adulten, gesunden, differenzierten Zellen detektierbar ist. Der humane Niedrig-Affinitätsrezeptor für IgE, auch bekannt als CD23, ist auf verschiedenen hämatopoetischen Zellen zu finden. Bei der B-CLL wird CD23 konstitutiv exprimiert und atypisch auf den malignen B-Zellen reguliert im Vergleich zu normalen B-Lymphozyten. Dies hat eine starke Überexpression des Moleküls zur Folge. Das Humane Ly9 oder CD229, das Homolog zum murinen Ly9, ist ein Mitglied der SLAM- (signaling lymphocyte activation molecule) Familie, die Singnalrezeptoren repräsentieren. CD229 interagiert mit Antigen-spezifischen Rezeptoren und vermittelt so die Zelladhäsion zwischen Lymphozyten und anderen Zellen. Die Überexpression von CD229 auf hämatopoetischen Zellen wurde in einer Publikation beschrieben, die gezeigt hat, dass 12/15 B-CLL Patienten positiv für das Molekül waren. Ein Merkmal eines Tumorantigens/TAAs stellt die Überexpression in einem Tumor im Vergleich zum Normalgewebe dar. Alle drei Antigene wurden bezüglich ihres Expressionsprofils untersucht, und es konnte eine eindeutige Überexpression verglichen mit normalen Zellen nachgewiesen werden (RT-PCR, FACS-Analysen). Die Immunogenität und MHC-Restriktion (hier HLA-A0201) der ausgewählten Peptide wurde mit Hilfe in vitro generierter zytotoxischer T-Zellen (CTLs) von gesunden Spendern gezeigt, die Antigen-spezifisch durch Stimulation mit autologen, Peptid-beladenen Dendritischen Zellen (DCs) expandiert wurden. Die endogene Prozessierung und Präsentation der potentiellen Tumorantigene, genauer der verschiedenen hTERT-, CD23- und CD229-entstammenden Peptide, konnte mit Hilfe Antigen-spezifischer CTLs von gesunden Spendern nachgewiesen werden, da diese spezifisch die HLA-A0201+ B-Zell-abstammende Zelllinie Ramos, HLA-A0201+ naive B-CLL Zellen und Peptid-beladene T2-Zellen in MHC-I-restringierter Weise erkannten (IFN-γ-ELISPOT Assays, [Cr51]-release Assay). Diese Experimente lassen auf eine natürliche Prozessierung und Präsentation der hTERT-, CD23- und CD229-entstammenden Peptide via HLA-A0201 auf den B-CLL Zellen schließen. Des Weiteren konnten autologe hTERT-, CD23- and CD229-spezifische T-Zellen von B-CLL Patienten in Gegenwart von autologen CD40L-aktivierten B CLL Zellen und interessanterweise auch durch autologe, naive, maligne B-Zellen expandiert werden, die einen deutlichen Anti-leukämischen Effekt zeigten (IFN-γ-ELISPOT Assays, Dimerfärbungen). Ein möglicher Grund für die Generierung einer T-Zellantwort mit autologen naiven B-CLL Zellen als Stimulatoren scheinen das von den B-CLL Zellen vermittelte Mikromilieu (Zytokinprofil) darzustellen. Anhand der Quantität und Qualität der erzielten CTL-Reaktivitäten gegen die drei unterschiedlichen Moleküle zeigte sich, dass vor allem die Oberflächenmoleküle CD229 und CD23 als neue TAAs bei der B-CLL geeignet scheinen. hTERT ist ebenfalls in der Lage, eine Antigen-spezifische T-Zellreaktion zu induzieren, jedoch mit geringerer Effizienz. Zusammenfassend konnte in dieser Arbeit gezeigt werden, dass CD229 und CD23 natürlich prozessiert und als TAAs bei der B-CLL präsentiert werden, die die Expansion autologer Tumor-spezifischer T-Zellen erlauben. Daher stellen sie geeignete Zielstrukturen für T-Zellbasierte, immuntherapeutische Strategien und ein Immunmonitoring bei dieser hämatologischen Erkrankung dar. Für hTERT gilt dies jedoch nur bedingt.

In situations where well-established approaches such as surgery, radiation therapy and chemotherapy fail to help cancer patients, immunotherapy has the potential to be an effective alternative. Tumour cells can sometimes be distinguished from corresponding normal cells due to their expression of tumour-associated antigens (TAAs), most of which are unaltered self-molecules. These molecules must be presented to the immune system in the context of danger in order to achieve their specific recognition. If dendritic cells (DCs), the most potent professional antigen-presenting cells, are loaded with RNA, they will translate the RNA into protein, process the protein into peptides and present the peptides within MHC molecules (pMHC) on their surface to cytotoxic T-lymphocytes (CTLs) and T-helper cells in a stimulatory manner. These effector cells can, in turn, recognise tumour cells. The goal of these studies was to find optimal conditions for producing a DC-based vaccine for cancer patients using TAAs in the form of RNA. The studies were designed to quantitate RNA transfer into DCs, to determine the intracellular stability of transfected RNA in DCs and to analyse the kinetics of protein expression and the generation of functional pMHC ligands that could activate effector memory CTLs. Simultaneous activation of CTLs with specificities for different antigens minimises the potential for tumour escape through immune selection of tumour variants showing loss of individual antigens. Thus, generation of multiplex pMHC ligands for CTLs may improve clinical efficiency. On the other hand, peptide competition for MHC molecules within the DC may limit pMHC ligand generation. This central immunological question was addressed by comparing DCs loaded with total cellular tumour RNA, amplified total cellular tumour mRNA and pools of defined single-species tumour-antigen cRNAs versus individual single-species tumour-antigen cRNAs for their capacity to display various pMHC ligands and activate CTLs of corresponding specificities. Experiments performed with RNA encoding the enhanced green fluorescence protein (EGFP), a reporter protein, showed that the highest efficiency of RNA transfection into DCs was achieved with electroporation, reaching levels of 90% positive cells. The fact that mature DCs expressed more EGFP than immature DCs suggests that this stage of DC maturation will be optimal for vaccine development. Importantly, electroporation and RNA transfer did not alter the expression of antigen-presenting and co-stimulatory molecules on the surface of DCs. The melanoma model was chosen for extensive analyses because its characterisation at the cellular and molecular levels has made it a very informative model for understanding cancer immunity. In addition to total cellular melanoma RNA, single-species cRNAs were used encoding the melanoma-associated antigens, tyrosinase, Melan-A and CDK4-R24C. Antigen presentation was detected with the help of effector memory CTL clones specific for each of these antigens. The CTL stimulatory capacity of RNA-transfected DCs was higher if they were allowed one day to recuperate from electroporation and to produce pMHC complexes. Tyrosinase cRNA dose-finding showed that more RNA would indeed result in higher stimulatory capacities of transfected DCs. Kinetics of tyrosinase cRNA degradation, similar to kinetics of EGFP cRNA degradation, revealed that the amount of transfected RNA rapidly decreased inside the DCs within 1.5 hr after electroporation. The smallest decrease was observed with the highest amount of RNA applied in electroporation. The kinetics of RNA degradation and protein half-life will be important parameters to consider in defining the right time-frame for T-cell activation by engineered DCs. When reverse transcription PCR (RT-PCR) was performed with total cellular melanoma RNA samples to generate amplified mRNA, the Melan-A, tyrosinase and CDK4/CDK4-R24C message was amplified 62-fold, 24-fold and 2-fold, respectively. These differences likely reflect variations in the expression levels of the corresponding antigen message in melanoma cells from which the RNA was isolated. Approximately 17240-fold more CDK4-R24C message, at least 500-fold more tyrosinase message and at least 480-fold more Melan-A message was found in single-species cRNAs when the same masses of single-species cRNA and amplified melanoma mRNA samples were compared. This explained why electroporation of single-species cRNAs into DCs yielded the highest DC stimulatory capacities. Combinations of tyrosinase, Melan-A and CDK4-R24C cRNAs were studied for their capacity to induce satisfactory levels of T-cell stimulation when presented by DCs. Here it was demonstrated that antigen competition was not a critical factor, since CTL responses to pooled RNAs were not inhibited even though competition for MHC class I molecules may have occurred within the DCs. DCs also developed CTL stimulatory capacities, but at much lower levels, using amplified melanoma mRNA. Two antigen-specific CTL clones displayed higher reactivities upon exposure to pMHC produced naturally by RNA-transfected DCs than to synthetic peptides pulsed onto DCs. In one case, this could be explained by a post-translational modification of the peptide, which normally occurs within cells. Since this particular modification was not represented in the synthetic peptide, which was chosen from the protein sequence, the synthetic peptide was not well recognised. This demonstrated that the use of RNA technology eliminates the need to know the correct sequences of immunogenic peptides. Thereby, DCs are better than scientists at choosing antigens and their epitopes for presentation to T-cells. These data provided a better understanding of antigen presentation by DCs based on the use of RNA, giving insight into antigen competition and paving the way for the use of pooled RNAs of defined species for the development of a multiplex vaccine. They also allowed a precise protocol for efficient T-cell activation to be defined. Further experiments will demonstrate whether quantitative differences detected in antigen presentation between DCs loaded with total cellular tumour RNA and amplified total cellular tumour mRNA versus single-species tumour-antigen cRNAs have an impact on de novo T-cell priming in vitro and in vivo.

Interactions between regulatory T lymphocytes and other cells are assumed to occur at the level of the cell surface. T cells which suppress the generation of specifically effector cells have been described as having antigenic, idiotypic, allotypic and I-region specificity1−4. Other T suppressor cells generated by in vitro cultivation with or without mitogenic stimulation5,6 have suppressive activity for T and B cells but no specificity can be assigned to them. These T suppressor cells (Ts) inhibit various lymphoid functions—this either reflects their polyclonal origin or indicates that the structures recognized by the Ts receptors must be common for many cell types. Carbohydrates on cell membrane-inserted glycoproteins or glycolipids might function as specific ligands for recognition by cellular receptors or soluble factors. Almost all cell-surface proteins of mammalian cells are glycosylated. There is evidence for lectin-like carbohydrate binding proteins not only in plants7 but also in toxins8, viruses9, prokaryotic cells10 and even mammalian cells, including T cells11. A functional role for these lectin-like proteins has been described for slime moulds and suggested for the selective association of embryonic cells12,13. We report here that addition of a monosaccharide can counteract the effect of T suppressor cells during the generation of alloreactive cytotoxic T cells (CTLs) in vitro.