Podcasts about b95

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Best podcasts about b95

Latest podcast episodes about b95

Lights Camera Jackson Podcasts
B95.5 — 2025 Oscars Predictions

Lights Camera Jackson Podcasts

Play Episode Listen Later Mar 2, 2025 6:53


I join host Jim Cerqua live on B95.5 (WYJB-FM) for 2025 Oscars predictions and analysis in some of the major categories, plus reflections on the careers of the late Gene Hackman and Michelle Trachtenberg.

The InEVitable
2024 Pebble Beach Car Week with Nilu27's Sasha Selipanov, Pininfarina's David Amante, & Lamborghini's Mitja Borket

The InEVitable

Play Episode Listen Later Aug 29, 2024 68:59


The future of hypercars! MotorTrend's Ed Loh & Jonny Liebermna take the show on the road to 2024 Pebble Beach Classic Car Forum for a panel discussion with three top designers - Sasha Selipanov (Founder/CEO Nilu27), Davide Amantea (Chief Design Officer Automobili Pininfarina), & Mitja Borkert (Design Director, Automobili Lamborghini)!0:33 - Pebble Beach Car Week!3:30 - Today's guests...5:43 - Panel introductions.7:22 - The InEVitable. Project 2036.8:52 - The Nilu Hypercar; defining the future of sports cars.12:46 - Pininfarina Battista & B95 inspired by Batman's Bruce Wayne.15:33 - Lamborghini Temerario.19:25 - The difficulty of the modern design process.22:40 - The rapid feedback loop of today.25:05 - Honoring heritage.27:26 - Pininfarina's Bruce Wayne car collab with Warner Brothers & DC Universe.30:11 - Legacy.38:23 - Does speed still define the hypercar?43:40 - Designing an experience.46:14 - Design principles.51:45 - Design & Engineering collaboration.58:32 - Favorite wedge car of all time?01:00:28 - Questions from the audience.

One Degree the Podcast
KANYE STOLE MY LINE | CK ON ONE DEGREE

One Degree the Podcast

Play Episode Listen Later May 9, 2024 84:20


The dynamic host of B95's Morning Juice crew and Fox Sports Radio, as he dives into the world of hip-hop, radio, and sports on this special episode. From dissecting the latest beef involving Kendrick Lamar  and Drake, to revealing the behind-the-scenes of his radio journey, CK shares intriguing insights and anecdotes. Discover how CK's encounters with famous personalities challenged his perceptions, and gain valuable motivation and inspiration from his unique perspective. Tune in for a captivating conversation that goes beyond the headlines, only on the One Degree Podcast.

This Could Get Me Fired
The Radio Life of CK - Episode: 127

This Could Get Me Fired

Play Episode Listen Later Mar 22, 2024 53:25 Transcription Available


He is the co-host of the "CK & CARMEN MORNING SHOW" on B95 in Fresno...Let's get to know about his wild radio journey. Ladies and gentlemen, CK.

SmartEducation Podcast
Onderneemster Stefanie Broes (Moonbird) vertelt hoe ademhaling kan worden ingezet als oplossing voor slaap- en stressproblemen

SmartEducation Podcast

Play Episode Listen Later Sep 5, 2023 43:18


In deze aflevering snijden we het onderwerp van innovatie in de zorg aan. Onze gast is niemand minder dan Stefanie Broes, de oprichter van Moonbird. Stefanie deelt haar persoonlijke verhaal en vertelt bevlogen over de missie die haar drijft in de wereld van de gezondheidszorg. Samen met haar broer Michael heeft ze een baanbrekend tactiel apparaat ontwikkeld dat fungeert als een ademcoach. Dit apparaat biedt begeleide ademhalingsoefeningen die individueel kunnen worden afgestemd en de voortgang kan worden bijgehouden via biofeedback in de bijbehorende app. Hoe werkt het, waar komt de naam Moonbird vandaan, voor wie? Stefanie onthult het mysterie achter deze intrigerende naam. Opvallend is ook het snelle traject van hoe Stefanie van idee naar een concreet product is gegaan. Luisteraars die zelf met een innovatief idee spelen, zullen zeker waardevolle tips oppikken voor hun eigen ondernemersreis. En opnieuw een winactie! We maken graag iemand blij die na het luisteren naar deze aflevering zelf met de Moonbird aan de slag kan gaan. https://www.moonbird.life/nl/ https://www.linkedin.com/in/stefanie-broes-58275b75/ https://en.wikipedia.org/wiki/B95_(red_knot) https://www.youtube.com/watch?v=P01HM6p6wp4 https://smarteducation.be/ https://smarteducation.be/zorg-doet-je-goed/ Surf naar https://smarteducation.be/stefanie-broes-in-de-zorg-doet-je-goed-podcast  en WIN!

CAL RADIO
DJ CAL Last Call w/ Visa on The Radio On B95 Fresno (04.26.22)

CAL RADIO

Play Episode Listen Later Apr 27, 2022 41:56


DJ CAL plays on B95 FRESNO and iHeart Radio. Hosted by Visa On Radio on the “Last Call” Weekday MixshowCatch him live 11PM PST on Tuesday & Thursday Nights on FM radio or on the iHeart Radio App!Enjoy my mixes & make sure to SHARE and leave a comment, let me know what you think or you want to hearYou can follow Visa on the radio on their socials @visaontheradioS T A Y C O N N E C T E DFacebook: www.facebook.com/DJCAL1/ Instagram: www.instagram.com/djcal1/ Twitter: www.twitter.com/dj_cal1

Fresno's Best
Monisha Mann, Case Manager for Equus Workplace Solutions and Former Radio DJ

Fresno's Best

Play Episode Listen Later Apr 14, 2022 48:23


Today, we have Monisha Mann on the show. Mann is a case manager for Equus Workplace Solutions where she helps people leaving the prison or the jail system find stable employment. She also was once the DJ on B95 in Fresno, working a radio DJ for 17 years. We had lots to talk about in terms of vulnerability, having a public image, bias, how to help former incarcerated transition to a more fulfilling life and more. Books: How to Do the Work: Recognize Your Patterns, Heal from Your Past, and Create Your Self by Dr. Nicole LePera Wendy's Got the Heat by Wendy Williams My Voice: A Memoir by Angie Martinez I'll Show You by Derrick Rose

CAL RADIO
DJ CAL Last Call w/ Visa on The Radio On B95 Fresno (01.04.22)

CAL RADIO

Play Episode Listen Later Jan 5, 2022 40:20


DJ CAL plays on B95 FRESNO and iHeart Radio. Hosted by Visa On Radio on the “Last Call” Weekday MixshowCatch him live 11PM PST on Tuesday & Thursdays Nights on FM radio or on the iHeart Radio App!Enjoy my mixes & make sure to SHARE and leave a comment, let me know what you think or you want to hearYou can follow Visa on the radio on their socials @visaontheradioS T A Y C O N N E C T E DFacebook: www.facebook.com/DJCAL1/ Instagram: www.instagram.com/djcal1/ Twitter: www.twitter.com/dj_cal1

CK & Carmen On Demand
CK talks with Fresno State women's soccer players Kassandra, Chais and head coach Brian

CK & Carmen On Demand

Play Episode Listen Later Aug 13, 2021 4:11


Fresno State celebration 100 years of Fresno State athletics and the first big celebration is with the Women's Soccer team as they play #2 Santa Clara...They joined CK and Carmen on B95!

CK On Sports
Nicole Lynn the first Black woman to represent a Top 3 NFL draft pick talks with CK

CK On Sports

Play Episode Listen Later Jul 12, 2021 8:52


CK On Sports had the pleasure of talking with Sports Agent Nicole Lynn about her rags to riches journey on becoming the first Black woman to represent a Top 3 NFL draft pick ...Listen to CK daily on B95 and Fox Sports Radio

CK On Sports
Clovis North vs Buchanan - The Rivalry CK talks w Coby Lindsey and James Gambrell

CK On Sports

Play Episode Listen Later Apr 9, 2021 7:42


Great time with these two awesome Athletic Directors, Coby Lindsey and James Gambrell as they get ready for the rivalry game tonight. They joined CK on B95!!!

CK & Carmen On Demand
DMX Dead at the age of 50...Gone far too soon!!!

CK & Carmen On Demand

Play Episode Listen Later Apr 9, 2021 6:42


One of the greats DMX dead at the age of 50...CK and Carmen on B95!

CK & Carmen On Demand
Celebrating International Women's Day with Her Body Bar, The Look Salon and Spoil Me Wax

CK & Carmen On Demand

Play Episode Listen Later Mar 8, 2021 12:23


Listen to Donetta from Her Body Bar, Veronica from The Look Salon and Isabel from Spoil Me Wax on International Women's Day on B95 with #CKandCarmen

body international women salon spoil celebrating international women's day b95
CK & Carmen On Demand
Celebrating International Women's Day with Julia Lopez from KSEE24/CBS 47, Fitness Sharan and MC JB from JJ Fad

CK & Carmen On Demand

Play Episode Listen Later Mar 8, 2021 22:02


Listen to Julia Lopez from KSEE24/CBS 47, Fitness Sharan and MC JB from JJ Fad on International Women's Day on B95 with #CKandCarmen

fitness international women lopez sharan celebrating international women's day jj fad b95
CK & Carmen On Demand
Celebrating International Women's Day with Margot from ABC30

CK & Carmen On Demand

Play Episode Listen Later Mar 8, 2021 13:24


Listen to ABC 30's Margot Kim on International Women's Day on B95 with #CKandCarmen

abc international women celebrating international women's day b95
CK & Carmen On Demand
Celebrating International Women's Day with MC JB from JJ FAD, Dez Washington, The Blink Studios and Aisha Nikole

CK & Carmen On Demand

Play Episode Listen Later Mar 8, 2021 26:47


Listen to MC JB from JJ FAD, Dez Washington, Candyce from The Blink Studios and Aisha Nikole on International Women's Day on B95 with #CKandCarmen

washington studios international women blink nikole candyce celebrating international women's day jj fad b95
Memories of a Moonbird
Patricia González, Biologist

Memories of a Moonbird

Play Episode Listen Later Jan 18, 2021 74:58


For more than 30 years, she’s been working in conservation to protect shore birds around the planet. She’s not only a Biologist and Field Ornithologist, but she's also the actual scientist who banded B95, the world famous Red Knot who was nicknamed "Moonbird," and whom my podcast and website were founded in honor of.Zooming in all the way from Argentina, she was kind enough to share with me the history of the real Moonbird, a look back on her career, and her views of the future of the Earth.Her name is Patricia González, and it’s my honor to welcome, on this very special 50th episode of the show, the fabulous woman known around the globe as the ”Mother of Moonbird.”Learn more about birds at https://www.birds.com/ and https://www.allaboutbirds.org/Patricia's Scientific Contributions at https://www.researchgate.netIf you enjoy this podcast, please leave a short review on Apple Podcasts by clicking here.Don't miss another episode! Sign up for the Moonbird email newsletter by clicking here!What about social media? Follow Daniel and the Moonbird Podcast:Instagram: instagram.com/memoriesofamoonbird/Facebook: facebook.com/MemoriesofaMoonBird/YouTube: youtube.com/MemoriesofaMoonBird/Twitter: https://twitter.com/moonbirdpodcastPinterest: pinterest.com/memoriesofamoonbird/You can also get early access to the episodes and support the show all-at-once! Click the link below!Support the show (https://www.patreon.com/moonbird)

Km42 - Running - Trail - Lifestyle
58. Courir tous les jours en juillet : bilan et réponses à vos questions

Km42 - Running - Trail - Lifestyle

Play Episode Listen Later Aug 5, 2020 65:46


Le mois de juillet est terminé depuis quelques jours et il est temps donc pour moi de faire un bilan de mon défi de courir tous les jours au mois de juillet. Et comme vous m’avez posé de nombreuses questions, je vais m’en servir comme fil rouge de cet épisode. Et je termine par mon conseil et ma « découverte » la plus importante.Les questions posées :Quelle était ta motivation première. Quelles ont été tes difficultés ?Combien de kilomètres au final ?As-tu alterné course longues et courses petites ou pas forcément ?Avais-tu un programme précis ou faisais tu au feeling ?Comment je me sens ?Est-ce que j’ai ressenti de la lassitude physique et mentale ?Pas de douleurs ?A quel moment tu as fait tes étirements ?As-tu eu des baisses de forme, si oui comment as-tu géré ?As-tu perdu du poids ?Tu recommandes ? Tu recommences ?Tu penses continuer en août ?As-tu envie de poursuivre l’expérience ?Ma routine de réveil articulaire : https://www.instagram.com/tv/B95-2-0nLK5/Mon astuce proprioception en me lavant les dents : https://www.instagram.com/tv/Bz0_Noin1v6/-----Soutenir le podcast via Patreon : https://www.patreon.com/km42Le site du podcast avec toutes les notes d’épisode et les archives : https://km42.runLa nouvelle chaîne YouTube Le Mouvement par Bertrand Soulier : http://bertrand.video/mouvementRejoindre le Hamsters Running Club : https://membres.hamstersrunning.club/————————Je m’appelle Bertrand Soulier. Il y a quelques années j’étais un gros hamster dépressif de 105 kilos. Depuis je fais du trail et j’ai couru mon premier marathon. KM42 est un podcast personnel sur ma vision du running et un mode de vie sportif. Je ne gagnerai jamais de course mais j’ai remporté la plus belle des victoires.Vous abonner sur iTunes : https://podcasts.apple.com/fr/podcast/km42-running-trail-lifestyle/id1427857153?mt=2Vous abonner sur Spotify : https://open.spotify.com/show/6xm6xOyAwrfjA729TlnNeE?si=2O-7vMwGRi6dVoYJ8JrxcgTwitter : http://twitter.com/bertrandsoulierMon blog : https://bertrandsoulier.comInstagram : https://www.instagram.com/bertrandsoulierMe retrouver sur Strava : https://www.strava.com/athletes/bertrandsoulierMusic from https://filmmusic.io"Upbeat Forever" by Kevin MacLeod (https://incompetech.com)License: CC BY (

CAL RADIO
DJ CAL - B95 - 4Th July Mix Fri & Sat

CAL RADIO

Play Episode Listen Later Jul 5, 2020 74:49


Here's my Fourth Of July Weekend mixes featured on B95Originally aired 07.04.2020Make sure to subscribe on Apple Podcasts and Mixcloud Select to stay up to date with future mixes.If you enjoyed the Mixes reach out to me on my socials @djcal1S T A Y C O N N E C T E Dwww.Deejaycal.comFacebook: www.facebook.com/DJCAL1/ Instagram: www.instagram.com/djcal1/ Twitter: www.twitter.com/dj_cal1

That's How You Feel? Podcast
Interview w/ B95 DJ and Republik Night Club owner, DJ Ren Rock

That's How You Feel? Podcast

Play Episode Listen Later Jun 26, 2020 57:07


Prince is frustrated with Don B sending his post-workout selfies. When playing Justin Bieber in the whip, don't disturb Prince or he's gonna be HEATED. Don B addresses why it's On Sight w/ "Karen's." The guys touch base on the MLB starting the season and Avery Bradley deciding not to play out the rest of the NBA season. Also, their thoughts on JR Smith possibly joining the Lakers.(31:00) We have a special guest joining the show for episode 6. Our guest is a DJ for popular 559 Hip-Hop & R&B radio show, B95. He also is the owner of one of the hottest nightclubs in the central valley, Republik, located in Visalia, Ca. DJ Ren Rock tells his story on how he got into DJing, building and operating his business, and meeting celebrities such as Drake and Kendrick Lamar. Dope interview! Make sure to follow our Instagram page, @thtshowyoufeel, to get up to date news in sports, new music droppin', future guests, and much much more!

THE TOP with Richie Phillips
Albany Radio Legends : WGNA's DICK STARK plus the UPSTATE DOWNSTATE SONG

THE TOP with Richie Phillips

Play Episode Listen Later Nov 18, 2019 38:00


It's Top Talent :Radio Legends -Series 1: Fun interview with old friend and former colleague from radio station WGNA in Albany NY, "Dick Stark After Dark".  There are fun anecdotes from our time on the air, we discuss the changes in the radio biz, famous folks we ment, plus there's a clip of a song he cowrote with his brother David - recorded in Nashville- PLUS special surprise guests, including some other legends-yes- my 17 year morning radio partner and great friend - SEAN MCMASTER from my years on the  SEAN AND RICHIE SHOW.  (Sean and wife Andrea are now on B95.5 in Albany).   We also hear from our old boss, former program director and radio icon RONALD "BUZZ" BRINDLE, PLUS a distraught listener of Dick's who was callously insulted by him on the air years ago.  He gets equal time on this podcast!    I also include at the end (36 minutes in) a novelty song that I wrote years ago to answer the question " Where's the better place to live- upstate or downstate New York? It's my "RHYMATORIAL" hopefully for your enjoyment!                          (New service! I'll write a song for that special someone -delivered by email- I call this "RichieRhymes".  Samples at the link below                             My website https://hirerichie.com/richie-rhymes                               Songs Dick has co-written with brother David :                            https://wwwreverbnation.com/davidstark/songs                            His bio link:                            https://www.linkedin.com/in/richardstarkcharlotte                    (New service! I'll write a song for that special someone -delivered by email- I call this "RichieRhymes".  Samples at the link below                             My website https://hirerichie.com/richie-rhymes                                                    

DJ Ren Rock's Podcast
DJ Ren Rock Block Party Mix 04

DJ Ren Rock's Podcast

Play Episode Listen Later Dec 20, 2018 76:13


Best of 2018 Sad! - Xxxtentacion Better Now - Post Malone Leave Me Alone - Flipp Dinero God's Plan - Drake Walk It Talk It - Migos ft. Drake Look Alive - BlocBoy JB ft. Drake Wake Up In The Sky - Gucci Mane, Bruno Mars & Kodak Black How Low FAED EDIT - Ludacris Nonstop - Drake Praise The Lord - Asap Rocky WORKINME - Quavo King's Dead - Jay Rock, Kendrick Lamar & Future Going Bad - Meek Mill ft. Drake Lucid Dreams - Juice WRLD Runaway NOVA EDIT - Kanye West Trip - Ella Mai B.I.D. - Tory Lanez Backin It Up - Pardison Fontaine ft. Cardi B One Kiss - Calvin Harris & Dua Lipa Electricity - Silk City I Found You - Benny Blanco & Calvin Harris Runaway DJ SCENE EDIT - Galantis Jackie Chan - Tiesto & Dzeko Mr. Brightside BEATBREAKER BOOTLEG - The Killers Win DJ SCENE EDIT - Jay Rock FEFE - 6ix9ine ft. Nicki Minaj I Like It - Cardi B, Bad Bunny & J Balvin Booty - Blac Youngsta No Stylist HMC EDIT - French Montana ft. Drake Sicko Mode - Travis Scott ft. Drake Flip The Switch - Quavo ft. Drake Boo'd Up CHIZZLE & REVER DEEP RMX - Ella Mai Gonna Love Me - Teyana Taylor Nice For What - Drake The Light - Ty Dolla Sign Twerk - City Girls ft. Cardi B Lemon - N.E.R.D. ft. Rihanna Ric Flair Drip - Offset & Metro Boomin Kika - 6ix9ine ft. Tory Lanez Uproar - Lil Wayne ZEZE - Kodak Black ft. Travis Scott & Offset In My Feelings - Drake Dura - Daddy Yankee Te Bote RMX - Nio Garcia, Darell, Casper ft. Bad Bunny, Nicky Jam & Ozuna Taki Taki - DJ Snake ft. Cardi B & Selena Gomez MIA - Bad Bunny ft. Drake Magenta Riddim - DJ Snake Taste - Tyga ft. Offset Big Bank - YG ft. 2 Chainz & Big Sean Finesse RMX - Bruno Mars & Cardi B Thank U, Next - Ariana Grande No Brainer - DJ Khaled ft. Justin Bieber

CK & Carmen On Demand
CK and Carmen Save Another Thanksgiving!!!

CK & Carmen On Demand

Play Episode Listen Later Nov 20, 2018 4:51


CK and Carmen Save Thanksgiving on B95!!! Check it out!!

CK & Carmen On Demand
#CKandCarmen's What's Trending 3/28

CK & Carmen On Demand

Play Episode Listen Later Mar 29, 2017 2:36


Find out the latest trends with CKandCarmen Daily on B95!!

DJ Ren Rock's Podcast
DJ Ren Rock Spring '14 Podcast #14

DJ Ren Rock's Podcast

Play Episode Listen Later Mar 17, 2014 48:44


www.DJRENROCK.com Fareast Movement - The Illest Beyonce - Drunk In Love DIPLO RMX Ta Ku - Electronic Relaxation J. ESPINOSA EDIT Milo & Otis - Love 2 Love U Jazmin Sisters - You Jhene Aiko ft. Kendrick Lamar - Stay Ready Beyonce - Partition Derek King ft. Kirko Bangz & Sage The Gemini - Jump YG ft. Drake - Who Do You Love Dillion Francis & DJ Snake - Get Low DJ Rapture ft. Lil Kee - Shake It Right Chris Brown - Loyal WEST COAST VERSION Savvy Sosa - Like I Mean It Adrian Marcel ft. The Kid Ryan - 2 AM TKR RMX The Kid Ryan - Run That Trey Songz - Na Na Raw Smoove ft. YG - Yo Goodie Showtek - We Like To Party Hard Rock Sofa & Eva Shaw - Get Down Pharrell - Happy YEE BOOTLEG The Chainsmokers - #Selfie ENCORE BOOTLEG Fedde Le Grand - Back & Forth GECKO BOOTLEG Syn Cole - Miami 82 AVICII RMX K Camp ft. Kwony Cash - Money Baby Drake - Trophies ELECTRIC BODEGA RMX Schoolboy Q - Studio Schoolboy Q - Man Of The Year Que - OG Bobby Johnson

DJ Ren Rock's Podcast
DJ Ren Rock Nov/Dec '13 Podcast #13

DJ Ren Rock's Podcast

Play Episode Listen Later Nov 19, 2013 31:36


www.DJRenRock.com Childish Gambino - 3005 Jhene Aiko - The Worst Drake ft. Jhene Aiko - From Time Eric Bellinger ft. Problem - I Don't Want Her Sage The Gemini - Don't You Justin Bieber - All Bad Tyga & Justin Bieber - Wait For A Minute Kid Ink ft. Chris Brown - Show Me Paul Wall ft. Kid Ink - Gettin' Tho'd TWRK - Nolia Clap Yo Gotti ft. Young Jeezy - Act Right Sean Kingston ft. Yo Gotti - Hold That Dev ft. Sage The Gemini - Kiss It Ty $ & Joe Moses ft. Kurupt - Tricks Jay-Z - Tom Ford Nipsey Hussle ft. Dom Kennedy - Checc Me Out

DJ Ren Rock's Podcast
DJ Ren Rock October '13 Podcast #12

DJ Ren Rock's Podcast

Play Episode Listen Later Sep 27, 2013 38:31


Krewella - Live For The Night W&W RMX Martin Garrix - Animals BOOTLEG Deorro - Yee EPIC REWORK Hardwell & W&W - Jumper Kaskade - Atmosphere GTA RMX Steve Angello - SLVR Fedde Le Grand - No Good BOOTLEG TJR - What's Up Suckaz BOOTLEG Capital Cities - Safe and Sound VICE RMX ATCQ - Scenario COVA & STEEL RMX Calvin Harris - I'm Not Alone Vice ft. Mike Taylor - World Is Our Playground DJ Snake ft. Lil Jon - Turn Down For What Kid Ink ft. Chris Brown - Show Me DJ Snake x Yellow Claw x Spanker - Slowdown Chris Brown ft. Nicki Minaj - Love More DJ Katch - The Horns Mike Will Made It - 23 CAKED UP RMX Diplo - Butter's Theme

DJ Ren Rock's Podcast
DJ Ren Rock Power 106 Jump Off Mix

DJ Ren Rock's Podcast

Play Episode Listen Later Jun 11, 2012 34:15


www.DJRENROCK.com Set 1 Calvin Harris ft. Ne-Yo-Let's Go Felli Fel-Boomerang Pitbull ft. Chris Brown-International Love JUMP SMOKERS RMX DJ Exodus-Put Yo Hands Up! The Wanted-Glad You Came Kid Ink-Time Of Your Life MIKE D RMX Jay-Z & Kanye West-In Paris Drake ft. Lil Wayne-HYFR Waka Flocka-Grove St. Party Kanye West-Mercy Set 2 Tyga-Betta Have My Money LIZ-Bounce E40-Function Chris Brown-Turn Up The Music MIAMI LIFE RMX Rihanna-Where Have You Been Cobra Starship-You Make Me Feel Kirko Bangz-Drank In My Cup MIKE D RMX Royal Society-Clap Your Hands Drake ft. Lil Wayne-The Motto MOOMBAHTON RMX The Finnaticz-Thun Thun Thun Travis Porter ft. Tyga-Ayy Ladies Tyga-Make It Nasty Rihanna ft. Chris Brown-Birthday Cake

Marathon TV
Extremlauf zur Euro2008 von München nach Klagenfurt über 454 Kilometer - 8.Tag

Marathon TV

Play Episode Listen Later Sep 19, 2008


Um 10:00 Uhr wurde wir mit Fanfaren in Mautendorf verabschiedet. Dort fand gerade ein Mittelalterfest statt. Die Strecke führte uns von Mauterndorf über Tamsweg über die B95 bis auf die Turracherhöhe. Die mit 1763 Meter der höchste Punkt unserer Gipfeltour nach Klagenfurt war. Nach erreichen der Turracherhöhe ging es wieder Bergab in Richtung Ebene Reichenau. Von starken Regenfällen begleitet trafen wir dann in Reichenau am Gemeindeamt ein. Die Streckenlänge betrug 56 Kilometer. Weitere Informationen über den lauf sind unter www.europameisterschaftslauf.de abrufbar.

Fakultät für Biologie - Digitale Hochschulschriften der LMU - Teil 02/06

Epstein-Barr Virus (EBV) is involved in several human malignancies via its latent gene products, which interact with cellular proteins and mimic discrete functions of cellular signalling pathways. Enigmatically, more than 90% of the human population carries this human tumour virus but virus-associated tumours are relatively rare. Most studies on EBV have been carried out in vitro and ex vivo on EBV-transformed human B cells or on human biopsies. Established in vivo model systems do not reflect the main aspects of EBV-associated diseases in humans. This limited tool set is the result of EBV’s inability to infect cells of non-human origin, which lack the surface receptor for EBV. My PhD work aimed at engineering a transgenic mouse, which carries a conditionally inactivated EBV genome. This study took advantage of the well-established techniques of mouse genetics in order to stably integrate the entire EBV genome into the murine genome. This approach would not only overcome the inability of EBV to infect animal cells but it would also permit to study the complete virus in an immunocompetent and easy-to-handle living organism. I undertook two routes to establish a transgenic mouse with the complete EBV genome inserted. One route was based on the site-specific integration into the hprt locus of murine embryonic stem cells. The other route engaged pronucleus microinjection of the EBV DNA into fertilized murine oocytes. In addition, the EBV genome was genetically manipulated prior to its introduction into murine cells. On the basis of the E.coli cloned EBV strain B95.8, I constructed a novel EBV mutant with unique features. This EBV targeting construct (InvTarg) allows conditional expression of EBV’s latent genes via a Cre/loxP system. Such approach prevents potentially adverse effects of EBV’s latent genes on embryonic development but allows their expression in almost any chosen cellular compartment for which specific Cre-expressing mice are available. The InvTarg recombinant EBV genome is 185 kb in size, based on a bacterial replicon, and therefore belonging to Bacterial Artificial Chromosomes (BACs). Two genetically modified and inversely oriented loxP sites were introduced in E.coli cells at the predetermined sites of the InvTarg, and the bracketed segment was inverted by Cre recombinase, disrupting transcription of almost all viral latent genes. In transgenic animals this inversion can be reverted and the latent genes can be re-activated at will by cross-breeding with Cre-expressing mouse (re-inversion). The ability of Cre to invert the big fragment was verified in infection experiments with human primary B cells. As expected, the ‘inverted’ EBV construct, such as InvTarg, failed to transform primary B cells, when the viral latent genes were not expressed. Despite sustained efforts, both gene delivery techniques did not lead to a transgenic mouse with the entire EBV genome inserted, but resulted in the integration of only subgenomic segments of the InvTarg recombinant EBV DNA. A number of technical problems were identified during this work, indicating more specific direction for further research. On the basis of the experience gained here, the project of an EBV transgenic mouse can be carried on. In addition, the InvTarg maxi-EBV conditional vector might be employed in other experimental conditions, like different cell types or distinct stages of cell differentiation, for studies on latent EBV genes.