Podcasts about luxr

Kate Rutter shares her thoughts on finding work in a challenging economy, how to design for great creative exchanges, and why she pushes back against authority. Highlights include: ⭐ How have you pushed back against overly rigid hierarchies? ⭐ Why did you take a part-time retail job at a retail store? ⭐ How does the quality of the question influence the quality of the idea? ⭐ Why isn't UX overly kind to late career-stage practitioners? ⭐ How are great creative exchanges like great sex? ====== Who is Kate Rutter? Kate is a Senior Adjunct Professor at California College of the Arts, where, for the past five years, she's been teaching undergraduates creativity and storytelling, and masters' students the foundations of experience design. She is also the Principal of Intelleto, the consulting practice through which she creates and facilitates visual explanations that make complex ideas simple, memorable and shareable. Before starting Intelleto, Kate pioneered the UX learning track at Tradecraft, an immersive learning program for product designers. She also co-founded the online education company Luxr.co, that helped early-stage entrepreneurs to find product/market fit. During her 20 years in the field, she's been a very generous contributor, including sharing her knowledge since 2015 through the “What's Wrong with UX” podcast, which she co-hosts alongside her good friend, Laura Klein.  ====== Find Kate here: LinkedIn: https://www.linkedin.com/in/katerutter/ Twitter: https://twitter.com/katerutter Website: http://intelleto.com/ ====== Liked what you heard and want to hear more? Subscribe and support the show by leaving a review on Apple Podcasts (or wherever you listen). Follow us on our other social channels for more great Brave UX content! YouTube: https://www.youtube.com/TheSpaceInBetween/ LinkedIn: https://www.linkedin.com/company/the-space-in-between/  Instagram: https://www.instagram.com/thespaceinbetw__n/  ====== Hosted by Brendan Jarvis: LinkedIn: https://www.linkedin.com/in/brendanjarvis/ Website: https://thespaceinbetween.co.nz/ Twitter: https://twitter.com/brendanjarvis/

In this brand new series (recorded a while ago so forgive the old LUXR intro), the Gang - Ellie, Jamie, Pablo and Skander - take on Jeff Gibbs and Michael Moore's 2019 film "Planet of the Humans".   Let's not kid ourselves. This film ruffled some feathers. We talk about what we think it got right and wrong, the back and forth between Gibbs/Moore and Mckibben from 350 org as well as how the film could've been improved.   Feel free to suggest a film we should review by email: risingwiththetide@gmail.com   Or any other social: Linktr.ee/risingwiththetide

*Episode 10 of our archived LUXR series*    In the final episode of our retired podcast, your hosts Skander and Jonny talks with Dr. Duncan McLaren, Professor in practice and research fellow at Lancaster University's LEC We discuss what carbon removal actually means, practically, its potential for positive change and climate outcomes, but also how companies have perverted the term for profit. Duncan tells us about his research into climate negotiations, climate justice and the uneven results of climate change, affecting the poorest first and hardest. Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next! 

 *Episode 9 of our archived LUXR series*    Your host Skander talks with Dr. Rebecca Whittle, human geographer and lecturer at Lancaster University. We discuss community gardening and alternative agriculture systems, her research on the topics as well as a more general discussion around emotional wellbeing: How do we deal with the emotional responses to climate change? How do we harness that raw emotional power but also how do we appease our fears and make peace with the nature that's all around us.   Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next! 

*Episode 8 of our archived LUXR series*   Your hosts Ellie and Skander talk with Joanna Haigh, retired Professor Emeritus at Imperial College London. Joanna tells us about her time as co-director of the Grantham Institute and as IPCC Lead Author, her career highlights and lows. We also discuss climate denialism, the relationships between science and government as well as geoengineering issues. Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!   

* Episode 7 of our old Podcast *   Your hosts Ellie and Skander talk with Gordon Walker, Professor in the University of Lancaster. For decades now, Gordon has researched the ins and outs of human/nature relationships. We discuss how to use natural energy flows, cycles and rhythms to best de-energise and de-carbonise our societies, as well as the effects of heat waves on care homes and the experience of flood impacts in the context of climate change.    Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next! 

* Ep 6 of our archived LUXR Series *   Your hosts Jonny and Skander talk with David Tyfield, Reader at the University of Lancaster. David's research focuses on critically analysing knowledge-based systems as well as mobility and climate issues in China and around the globe. We discuss the complex role of China in the climate crisis, science's obessession with techne/episteme and how to build a better scientific method.  Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!

*Ep. 5 of our archived LUXR series*    Your hosts Jamie and Skander talk with lecturer in International Relations at the School of Global Studies for the University of Sussex, Dr. Andrea Brock. Andrea researches political ecology, with a particular focus on exctractivism, environmental/social justice and the violence associated with environmental developments. We discuss her expertise on the decarbonisation divide, the dangers of offsetting and the road to a better world for all of us.   Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!

*Ep. 4 of our archived LUXR series*    Your hosts Jamie and Skander talk with postdoctoral fellow at the Center for Development and Environment (SUM) for the University of Oslo, Dr. Alexander Dunlap. Alexander specialises in critical anthropology and political ecology and talks to us about the negative socio-economic impacts of wind energy developments, resistance in the face of land-grabbing and corruption around mining operations. Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!

*Ep. 3 of our archived LUXR series*    Your hosts Josh and Skander talk with Lancaster University Senior Lecturer Dr. Benjamin Neimark who specialises in political ecology. Ben talks to us about bioprospecting in Madagascar, the fuel emissions and carbon "boot-print" of the US Military as well as his views on environmental movements at LU and abroad.    Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!

*Ep. 2 of our archived LUXR series*    Your hosts Pablo and Skander interview Lancaster academic Petter Terenius who specialises in Ocean Thermal Energy Conversion and Renewable Energy solutions. Petter teaches us about the potential of OTEC and of using heat from data centers to power generators. We delve into the relationship between tech and climate, as well as a bit of eco-minded philosophy.  Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!  Love and Rage

*Ep. 1 of our archived LUXR series*  Your hosts Ellie and Skander talk with Lancaster University Teaching Fellow and UCU Branch Secretary Dr. Emily Heath who specialises in earth sciences. In our first ever episode, we discuss the current environmental scene and situation in Lancaster, and learn from Emily's years of fighting for change at and outside of the University. Let us know your thoughts at risingwiththetide@gmail.com as well as what you'd like us to talk about next!

Kate Rutter is an adjunct professor of design at the California College of the Arts and a principal at Intelleto, with decades of experience in product design and management, infusing time at Adaptive Path and Luxr. In this episode of the Product Science Podcast, we talk about how to hire for right traits, work-life balance, and how to adapt a hacker/DIY ethos in your work. Read the show notes to learn more.

Working on teams is great because we get to share our skill sets and learn from one another as we build. However, there are moments that prove to be challenging like… … when you think you’re in agreement with your team, get to work, then days later the decision gets shot down. All that wasted time, energy, and effort! Or… … when you’re excited to try something new, but you can’t because you have to get approval from multiple people, some who don’t even know who you are! Dealing with the all the drama can be demotivating and stifle innovation. So how do you get groups people out of their own way in order to make decisions, deliver on innovations, expose weaknesses in strategy, and is it even possible? Yes, it is and my goal in today’s episode is to show you how! I’ve invited Janice Fraser who is the Director on the People Team at Pivotal Labs. Janice has a rich 16-year career with notable accomplishments such as being the CEO and Founder of Luxr, CEO of Adaptive Path, and more. We’ll be focusing the conversation on showing how to govern teams that are self-governing through a concept known as balanced teams, and much of what we’re going to be showcasing is based on the principles and practices of Extreme Programming  by Kent Beck. While much of what we’ll cover applies to building software products, Janice has also applied it to creating policies and processes for enterprises and the Obama administration. This is a really meaty episode, where we’ll be getting into the weeds and covering: How to make decisions in the face of inadequate information How the old way of creating limited ownership and understanding, and balanced teams have shared ownership based on vertical and horizontal expertise How extending an invitation to NOT buy releases pressure from a team feeling like they need to change or adopt new approaches immediately Why decisions often don’t stick and how the UBAD model (understanding, belief, advocacy, decision) leads to decision making that lasts How to create your “Island of Freedom” where you can have autonomy with boundary conditions you’ve agreed upon with your team and boss Why trust is a two-way street: gaining the trust to experiment and sharing news even if it’s bad Why trust includes a safety net of knowing that people are going to make mistakes If you’ve been wondering how to make a shift in your organization, and have struggled to get people onboard, then you’ll want to watch this episode! One of my favorite quotes from Janice from the episode is the following: “Agreement happens when people agree to stop talking. People stop talking when they feel understood.  Understanding happens between individual pairs of brains.” __  FemgineerTV is produced as a partnership between Femgineer and Pivotal Tracker. San Francisco video production by StartMotionMEDIA.

Bacteria constantly need to monitor their environment and adapt the bacterial group-coordinated behaviour to changing habitats like nutrition alterations or host variations. Commonly cell-cell communication via acyl homoserine lactones (AHLs)is used to synchronise the behaviour of a bacterial population dependent on cell size. This process is referred to as quorum sensing (QS) and predominantly occurs in Gram-negative bacteria. The typical QS system consists of a LuxI-synthase that synthesises AHLs, and a LuxR-type receptor, which then responds to these AHLs. Upon AHL-binding, the LuxR-type receptor regulates the expression of different target genes and thus influences several processes, like biofilm formation, virulence, antibiotic production or cell-cell interaction. Interestingly, many proteobacteria possess additional LuxR homologs, but lack a cognate LuxI-type synthase. Those LuxR-type receptors are referred to as LuxR orphans or LuxR solos and can expand the regulatory QS network. Photorhabdus species are insect pathogenic bacteria, living in symbiosis with entomopathogenic nematodes. They all possess an exceptionally high number of LuxR solos, but lack LuxI homologs and therefore do not produce AHLs. The function of these LuxR solos, their role in cell-cell communication and the identification of their cognate signalling molecules in Photorhabdus species is the main focus of this work. In this thesis a novel signalling molecule used for QS could be identified for the first time in P. luminescens. This novel QS molecule is an α-pyrone named photopyrone (PPY) and produced endogenously by the photopyrone synthase (PpyS). The PPYs are specifically recognized by the LuxR solo regulator PluR, which then activates expression of the pcf (Photorhabdus clumping factor) operon leading to cell clumping of P. luminescens cells. Moreover, the PpyS/PluR quorum sensing system and its induced cell clumping contribute to the overall toxicity of P. luminescens. Furthermore, a second novel signalling molecule sensed by a LuxR solo of Photorhabdus species could be identified besides PPYs. The insect and human pathogenic bacteria P. asymbiotica lacks a PpyS homolog as well as a LuxI homolog, but harbours a pcf operon and a homologue to PluR, which is named PauR. The signalling molecule sensed by the LuxR-type receptor PauR could be identified, which is neither an AHL nor a PPY. PauR recognises a 2,5-dialkylresorcinol (DAR) produced by the DarABC pathway. Upon binding of the cognate signalling molecule, Summary XII PauR activates expression of the pcf operon. This also leads to cell clumping in P. asymbiotica. Furthermore, the DarABC/PauR QS system also contributes to the overall pathogenicity of P. asymbiotica against Galleria mellonella insect larvae. A bioinformatics approach revealed a high number of LuxR solos present in P. temperata and P. asymbiotica like in P. luminescens. Thereby, several conserved motives of amino acids could be identified, which are potentially important for signalbinding and -specificity. Variations in these amino acid motifs are assumed to reflect the overall variety of signals that can be sensed by LuxR solos. Furthermore, the specificity of the two LuxR solos PluR and PauR towards their cognate signalling molecules, PPYs and DARs, respectively, was analysed. Thereby, it could be shown that the previously identified conserved amino acid motives in the signal-binding domain (SBD), the TYDQCS-motif of PluR and the TYDQYI-motif of PauR, are essential but not sufficient for ligand-binding. Similar as for AHLs, it was unclear how the signalling molecules PPYs and DARs can cross the bacterial cell membrane. In the last part of this thesis the import mechanism for the Photorhabdus-specific signalling compounds PPYs and DARs were identified. Initial evidence could be provided that the membrane-integrated transporter FadL is mainly involved in the import of these hydrophobic compounds, and that they are not transported via simple diffusion across the cell membrane, which is assumed for AHLs. In conclusion, the data that is compiled presents two LuxR solos of Photorhabdus species adapted to sense and respond to novel non-AHL signalling molecules used for QS. Therefore, this thesis reveals that cell-cell communication via LuxR-type receptors goes far beyond AHL-signalling in nature.

Bacteria produce and excrete signaling molecules, so called autoinducers, which allow them to monitor their population density and/or their environment in a process best known as quorum sensing. The Gram-negative marine bacterium Vibrio harveyi regulates certain virulence factors like type III secretion, siderophore production, and exoproteolytic activity as well as biofilm formation and bioluminescence using quorum sensing. The bacterium produces three different autoinducers: HAI-1, a N-(3-hydroxybutyryl)-D-homoserine lactone, AI-2, a furanosylborate diester, and CAI-1, a (Z)-3-aminoundec-2-en-4-one. The autoinducers are recognized by the hybrid sensor kinases LuxN, LuxQ and CqsS. All information is transferred to the phosphotransfer protein LuxU and the response regulator LuxO via phosphorelay and further transduced into the copy number of the master regulator LuxR. LuxR induces/represses a multitude of genes/operons (>100) including the lux-operon responsible for the production of bioluminescence. In order to understand how single cells behave within an autoinducer-activated community, autoinducer-induced processes were investigated in a homogeneous environment over time. Analysis of wild type single cells with respect to bioluminescence revealed that even at high cell densities only 70% of the cells of a population were bright. Moreover, fractionation of the population was found for autoinducer-controlled promoters (of genes coding for bioluminescence, exoproteolytic activity, and type III secretion) using reporter strains containing promoter::gfp fusions. These results indicated phenotypic heterogeneity of a genetic homogeneous population and were independent of the used cultivation medium, temperature or strain. An artificial increase of the autoinducer concentrations resulted in an all-bright cell population similar as observed for a luxO deletion mutant. Both, wild type and deletion mutant switched to biofilm formation at high cell density. However, the capability of the mutant to produce biofilm was significantly reduced. These data suggest that a population of the non-differentiating bacterium Vibrio harveyi takes advantages of division of labor. In addition, a temporal variation of the autoinducer concentrations over time was found. The extracellular concentrations of the three autoinducers and quorum sensing-regulated functions of Vibrio harveyi were monitored in a growing culture. In the early and mid-exponential growth phase only AI-2 was detectable and bioluminescence was induced. In the late exponential growth phase both, HAI-1 and AI-2 reached their maximum values, bioluminescence stayed high and exoproteolytic activity was induced. The stationary phase was characterized by equal concentrations of HAI-1 and AI-2, exoproteolytic activity reached its maximum, and CAI-1 activity was detectable in the culture fluids. Furthermore, only a stable and mature biofilm was formed, when HAI-1 and AI-2 were present in the above described ratios over time. CAI-1 had no influence on the biofilm formation in Vibrio harveyi. These results demonstrate that not the cell density per se is important, but that autoinducers rather control the development of a Vibrio harveyi population.

Quorum Sensing (QS) is a process that enables bacteria to communicate via chemical signalling molecules, which are called autoinducers (AI). When a threshold concentration of QS molecules is reached, the bacteria start their QS regulated gene expression e.g. bioluminescence (Vibrio fischeri), virulence factor secretion (Pseudomonas aeruginosa), biofilm formation (Burkholderia cepacia), sporulation, and mating. It was found that many Gram-negative bacteria use acylated homoserine lactones (AHLs or HSLs) as autoinducers. Due to the broad biological functions of HSLs, the interest in detection and analysis of HSLs is increasing for medical, biotechnological and agricultural applications. In the past years, numerous analytical methods have been developed for HSLs. Conventional analysis, which usually combines chromatography, mass spectrometry (MS), and nuclear magnetic resonance (NMR) has been very successfully applied for identification and quantification of HSLs. But normally, conventional analysis requires many steps of sample preparation, e.g. extraction, pre-concentration and optimisation of conditions to separate individual HSL molecules. In addition, many sensitive bioreporter assays have been developed using different LuxR responsive promoters, which contain LuxR family functional proteins but lack the HSL synthase. A combination of different bioassays is strongly recommended, since no bioreporter is sensitive to all HSLs. Alternatively, in this study, an anti-HSL antibody based immunochemical detection method has been successfully developed and established. HSL molecules consist of a homoserine lactone ring and an acyl side chain (4-18 carbon atoms), and they differ only from side chain length and substitution at C3 atom. Regarding the variation of the molecule structures, four HSL haptens, named HSL1, HSL2, HSL3 and HSL4, were designed for antibody and assay development. HSL1 and HSL3 have a long chain (C11-COOH), but HSL1 has an -oxo and HSL3 has an –OH functional group at the C3 position. In comparison, HSL2 (C5-COOH) and HSL4 (C9-COOH) have shorter side chains and no substitution on the C3 atom. The haptens were synthesised and were covalently coupled to the C-terminal COOH-group of the NH2-residues (lysines) of the carrier proteins (BSA/OVA). Using these HSL hapten-conjugates, rat and mouse anti-HSL monoclonal antibodies (mAbs) were produced, screened and further characterised with enzyme-linked immunosorbent assays (ELISAs). Corresponding to hapten structures, the antibodies showed different selectivities to HSLs with different substitution on C3 position and chain length. Eight mAbs (HSL1-1A5, HSL1-8E1, HSL1/2-2C10, HSL1/2-4H5, HSL4-4C9, HSL4-5H3, HSL4-5E12 and HSL4-6D3) were selected from about 200 mAbs and characterised in detail using coating antigen and enzyme tracer formats. It was demonstrated that the new assays have HSL detection ranges from nM to low µM, which is sensitive enough for detection of HSLs in natural samples according to literature. Interestingly but not surprisingly, AHLs mAbs have at least 20 times higher sensitivity against hydrolysed HSLs (named HSs) than original HSLs, because the conjugation and immunisation conditions, e.g. pH and temperature, for mAb development resulted in HSL hydrolysis. This property of antibodies additionally offers a new sensitive method to detect quorum quenching (QQ) relevant homoserines (HSs), which are important degradation products of HSLs. Comparable results have been obtained by Biacore and Aqua-Optosensor biosensors for HS (L) characterisation. Based on these properties of the mAbs, a detection method of HSLs and HSs in biological samples has been developed and optimised. With the comparison of the real samples before and after hydrolysis treatment, the assays could simply present the relative HSL- and HS- contents in the samples. Similar to bio-reporters, the identification or quantitation of single HSL molecules is not possible only using immunoassay due to the broad recognition of HSLs and HSs. For this purpose, a combination with conventional chemical analysis is a must. However, as a novel sensitive HSL and HS detection method, the developed immunoassays have the advantages of being fast, cost effective and having low sample volume requirement. Using the direct or indirect fluorescence signals of fluorophore labelled anti-HSL mAbs, the in situ experiments with modified Burkholderia cepacia biofilm on ibidi slide (plastic flow chamber from ibidi GmbH) and Pseudomonas putida inoculated barley root have been carried out. Unfortunately, the in situ tests were not successful, mainly due to remaining unspecific background signals. Nevertheless, a few steps, e.g. fluorophore labelling, biofilm formation, and surface blocking have been optimised. The door of HSL in situ tests with the antibodies is still open, if a suitable specific visualising detection method could be found in the future. Certainly, the antibodies can also be broadly applied for many other immunochemical techniques, such as immunosensors or immunoaffinity columns for characterisation or pre-screening of HSLs/HSs, as have been demonstrated successfully.

Die Signaltransduktionskaskade des komplexen Quorum sensing-Systems in Vibrio harveyi umfasst die drei Hybridsensorkinasen LuxN, LuxQ und CqsS, das Histidinphosphotransferprotein LuxU und den Antwortregulator LuxO. Bei niedriger Zelldichte funktionieren die Hybridsensorkinasen als Autokinasen. Die Phosphorylgruppe wird zunächst intramolekular übertragen und anschließend auf LuxU und LuxO weitergeleitet. Phosphoryliertes LuxO aktiviert die Expression von fünf regulatorischen RNAs, die im Zusammenspiel mit dem RNA-Chaperon Hfq die Translation der mRNA des Masterregulators LuxR inhibieren. Bei hoher Zelldichte wird die Kinaseaktivität der Hybridsensorkinasen durch die jeweiligen Autoinduktoren (LuxN: HAI-1, LuxQ: AI-2, CqsS: CAI-1) inhibiert, sodass es zum Abschalten der Phosphorylierungskaskade und zur Anreicherung von LuxR kommt. Im Rahmen dieser Arbeit wurden die Proteine LuxN und LuxO biochemisch näher charakterisiert. Mit Hilfe diverser Methoden konnte die Topologie des Membranproteins LuxN, zusammen mit der Lage des N-Terminus, gelöst werden: das Protein besteht aus neun Transmembrandomänen mit einem periplasmatisch lokalisierten N-Terminus. Im Zuge der biochemischen Charakterisierung von LuxN wurden zwei an der Bindung des Autoinduktors HAI-1 beteiligte Aminosäuren identifiziert. Das Membranprotein LuxN wurde erfolgreich gereinigt und in Escherichia coli- und V. harveyi-basierten Proteoliposomen rekonstituiert. Ebenso wurde der in Form von Inclusion Bodies heterolog in E. coli überproduzierte Antwortregulator LuxO gereinigt und renaturiert. Das renaturierte Protein konnte erstmalig mit dem niedermolekularen Phosphodonor [γ-32P]-Acetylphosphat markiert werden und eine Bindung an die DNA in phosphorylierter und nicht-phosphorylierter Form im Bereich der hypothetischen σ54- und LuxO-Bindestelle gezeigt werden.

Background: Photorhabdus luminescens and Yersinia enterocolitica are both enteric bacteria which are associated with insects. P. luminescens lives in symbiosis with soil nematodes and is highly pathogenic towards insects but not to humans. In contrast, Y. enterocolitica is widely found in the environment and mainly known to cause gastroenteritis in men, but has only recently been shown to be also toxic for insects. It is expected that both pathogens share an overlap of genetic determinants that play a role within the insect host. Results: A selective genome comparison was applied. Proteins belonging to the class of two-component regulatory systems, quorum sensing, universal stress proteins, and c-di-GMP signalling have been analysed. The interorganismic synopsis of selected regulatory systems uncovered common and distinct signalling mechanisms of both pathogens used for perception of signals within the insect host. Particularly, a new class of LuxR-like regulators was identified, which might be involved in detecting insect-specific molecules. In addition, the genetic overlap unravelled a two-component system that is unique for the genera Photorhabdus and Yersinia and is therefore suggested to play a major role in the pathogen-insect relationship. Our analysis also highlights factors of both pathogens that are expressed at low temperatures as encountered in insects in contrast to higher (body) temperature, providing evidence that temperature is a yet under-investigated environmental signal for bacterial adaptation to various hosts. Common degradative metabolic pathways are described that might be used to explore nutrients within the insect gut or hemolymph, thus enabling the proliferation of P. luminescens and Y. enterocolitica in their invertebrate hosts. A strikingly higher number of genes encoding insecticidal toxins and other virulence factors in P. luminescens compared to Y. enterocolitica correlates with the higher virulence of P. luminescens towards insects, and suggests a putative broader insect host spectrum of this pathogen. Conclusion: A set of factors shared by the two pathogens was identified including those that are involved in the host infection process, in persistence within the insect, or in host exploitation. Some of them might have been selected during the association with insects and then adapted to pathogenesis in mammalian hosts.