Podcasts about pc4

Michael Freedman is a mathematician who was awarded the Fields Medal in 1986 for his solution of the 4-dimensional Poincare conjecture. Mike has also received numerous other awards for his scientific contributions including a MacArthur Fellowship and the National Medal of Science. In 1997, Mike joined Microsoft Research and in 2005 became the director of Station Q, Microsoft's quantum computing research lab. As of 2023, Mike is a Senior Research Scientist at the Center for Mathematics and Scientific Applications at Harvard University. Patreon (bonus materials + video chat): https://www.patreon.com/timothynguyen In this wide-ranging conversation, we give a panoramic view of Mike's extensive body of work over the span of his career. It is divided into three parts: early, middle, and present day, which respectively include his work on the 4-dimensional Poincare conjecture, his transition to topological physics, and finally his recent work in applying ideas from mathematics and philosophy to social economics. Our conversation is a blend of both the nitty-gritty details and the anecdotal story-telling that can only be obtained from a living legend. I. Introduction 00:00 : Preview 01:34 : Fields Medalist working in industry 03:24 : Academia vs industry 04:59 : Mathematics and art 06:33 : Technical overview II. Early Mike: The Poincare Conjecture (PC) 08:14 : Introduction, statement, and history 14:30 : Three categories for PC (topological, smooth, PL) 17:09 : Smale and PC for d at least 5 17:59 : Homotopy equivalence vs homeomorphism 22:08 : Joke 23:24 : Morse flow 33:21 : Whitney Disk 41:47 : Casson handles 50:24 : Manifold factors and the Whitehead continuum 1:00:39 : Donaldson's results in the smooth category 1:04:54 : (Not) writing up full details of the proof then and now 1:08:56 : Why Perelman succeeded II. Mid Mike: Topological Quantum Field Theory (TQFT) and Quantum Computing (QC) 1:10:54: Introduction 1:11:42: Cliff Taubes, Raoul Bott, Ed Witten 1:12:40 : Computational complexity, Church-Turing, and Mike's motivations 1:24:01 : Why Mike left academia, Microsoft's offer, and Station Q 1:29:23 : Topological quantum field theory (according to Atiyah) 1:34:29 : Anyons and a theorem on Chern-Simons theories 1:38:57 : Relation to QC 1:46:08 : Universal TQFT 1:55:57 : Witten: Donalson theory cannot be a unitary TQFT 2:01:22 : Unitarity is possible in dimension 3 2:05:12 : Relations to a theory of everything? 2:07:21 : Where topological QC is now III. Present Mike: Social Economics 2:11:08 : Introduction 2:14:02 : Lionel Penrose and voting schemes 2:21:01 : Radical markets (pun intended) 2:25:45 : Quadratic finance/funding 2:30:51 : Kant's categorical imperative and a paper of Vitalik Buterin, Zoe Hitzig, Glen Weyl 2:36:54 : Gauge equivariance 2:38:32 : Bertrand Russell: philosophers and differential equations IV: Outro 2:46:20 : Final thoughts on math, science, philosophy 2:51:22 : Career advice   Some Further Reading: Mike's Harvard lecture on PC4: https://www.youtube.com/watch?v=TSF0i6BO1Ig Behrens et al. The Disc Embedding Theorem. M. Freedman. Spinoza, Leibniz, Kant, and Weyl. arxiv:2206.14711   Twitter: @iamtimnguyen   Webpage: http://www.timothynguyen.org

Unplanned extubation in the PCICU patient is a serious concern with many untoward associations. Who is at highest risk for this complication? Why is this complication associated with worse outcomes in general? How does race or ethnicity impact the likelihood of this complication? We speak with Assistant Professor of Pediatrics at the University of Cincinnati and pediatric cardiac critical care expert, Dr. Tanya Perry about a recent PC4 registry study on unplanned extubation in the PCICU. doi: https://doi.org/10.1097/pcc.0000000000003235

Nikki Davis speaks with Dr Gemma Skaczkowski, a Research Fellow from the University of South Australia, about her systematic review: The nature and impact of patient and public involvement in cancer prevention, screening and early detection research. Nikki Davis is a member of PC4's consumer community and is our guest host for this episode. In this conversation they discuss: -What constitutes patient involvement -What research stage most commonly involves consumers -And the benefits of patient and consumer involvement This review found that involvement is increasing but more involvement is needed across each step of the research process. You can read the full review here: https://www.sciencedirect.com/science/article/abs/pii/S0091743522004777?via=ihub

#自作PC #ゲーミングPC #パーツ構成 #人生初 #ポッドキャスト #クドラジ #Podcast #kudoradi 本日の一言「お金も時間もかなりかかったけど、PCに詳しくなったし組み立てはめちゃくちゃ楽しかった！PCの動作も格段に良くなったので非常に満足しています！！」 --- ▼関連サイト パーツ構成 CPU：intel インテル CPU 第13世代 Core i7-13700K CPUクーラー：Noctua NH-U12A chromax.black, 120mm シングルタワー CPUクーラー (ブラック) マザーボード：ASUS INTEL 第12世代・13世代CPU(LGA1700)対応Z790チップセット ATX マザーボードTUF GAMING Z790-PLUS D4 メモリ：シー・エフ・デー販売 CFD販売 CFD Standard デスクトップ用 メモリ DDR4 3200 (PC4-25600) 16GB×2枚 288pin DIMM 相性保証 W4U3200CS-16G SSD：ウエスタンデジタル Western Digital SN850X 1TB ヒートシンク搭載【PS5動作確認済み】PCIe Gen4×4 NVMe (読取り最大 7,300MB/秒) 内蔵SSD WDS100T2XHE-EC GPU（グラフィックカード）：ASUSTek GeForceRTX 3080 搭載 V2 OC Edition 10GB GDDR6X TUF-RTX3080-O10G-V2-GAMING ケース：Fractal Design Define 7 Black TG ミドルタワーPCケース E-ATX 対応 強化ガラス(ダーク)モデル 電源：エフエスピー 80 PLUS GOLD 規格 ATX 電源 セミモジュラー タイプ Hydro GT PROシリーズ 850W [ HGT-850 ] ブラック --- ▼関連エピソード Kudo、自作PC組むってよ。その他、アニメ・ゲーム・映画の話題も。 --- ▼お便りフォーム（Message Form） こちら からクドラジへお便りを送ることができます。意見、感想、質問、アドバイス、どんなものでも構わないので気軽に送ってみてください！ --- ▼週間アニメ感想らじお（仮） with ぽぽくりーむ 編集版：Amazon Music・Apple Podcasts・Spotify Live：Radiotalk --- ▼Kudo's Games ゲーム実況動画も配信中！現在配信中のタイトル↓ ヘブンバーンズレッド、英雄伝説 黎の軌跡II -CRIMSON SiN-、モノクロームメビウス 刻ノ代贖 --- ▼クドラジサポート（Kudo's Radio Support） こちらからクドラジの活動をサポートすることができます。100円からサポートが可能です。 --- ▼Amazon Music Unlimited 1億曲以上が"自由"に聴き放題！HD・ULTRA HD音質での視聴も可能！Dolby Atmos・360 Reality Audioによる空間オーディオ体験も！無料体験に登録 してクドラジと推しのアーティストを応援しよう！ --- ※AmazonMusicPartner & AmazonアソシエイトのリンクURLにより収入を得ています。 その他各種リンクはこちら から！（Other links）

In this episode Kristi Milley continues her conversation with Professor Jon Emery, Herman Chair of Primary Care Research at the University of Melbourne, and Director of PC4. And Professor Michael Jefford, Medical Oncologist and Director of the Australian Cancer Survivorship Centre at the Peter MacCallum Centre. Jon and Michael are part of a team of experts who have published a series of three papers around Cancer Survivorship in the Lancet. And for those of you who aren't in research, The Lancet would be the silver medallist at the Olympics in medical journals. Today, we're discussing the first two papers in the series: management of clinical issues experienced by survivors, and improved models of care for cancer survivors.

Today we are speaking with Professor Jon Emery, Herman Chair of Primary Care Research at the University of Melbourne, and Director of PC4. And Professor Michael Jefford, Medical Oncologist and Director of the Australian Cancer Survivorship Centre at the Peter MacCallum Centre. Jon and Michael are part of a team of experts who have published a series of three papers around Cancer Survivorship in the Lancet. And for those of you who aren't in research, The Lancet would be the silver medallist at the Olympics in medical journals. Today, we're discussing the first two papers in the series: management of clinical issues experienced by survivors, and improved models of care for cancer survivors.

Sarah Tabbutt, MD, PhD (UCSF Benioff Children's Hospital) and Angela Bates, MD (University of Alberta Stollery Children's Hospital) discuss the current state and future of medical registries in pediatric cardiac critical care in context of their work with PC4 and PPHNet and how to leverage registries for return on investment. Host/Editor: Raj Sahulee, DO (NYU Hassenfeld Children's Hospital); Producer: David Werho, MD (UC San Diego).

Look out …it's a HOST SWAP! member of the PC4 Community Advisory Group; Louise Bailey took reign of the microphone in this episode of Research Round-up to interview our own PC4 National Manager Dr Kristi Milley and Ms Sophie Chima from PC4 about their new paper published in the journal Health Expectations, Long-term consumer involvement in cancer research: Working towards partnership. Show notes are located here: http://pc4tg.com.au/research-round-up-july-2021-with-special-host-louise-bailey-and-guests-dr-kristi-milley-sophie-chima

The boys hop on to talk PC4, Team Challenge, and The Road Trip Snack Draft plus ALOT MORE!!! Follow FlowTKast: https://linktr.ee/flowtkast  Support FlowTK: https://flowtkallday.com  Support the PODCAST: https://www.patreon.com/Flowtkast --- Support this podcast: https://anchor.fm/flowtkast/support

AtrociousGameplay joins JW for this week's episode of Tag Team! Follow the two as they talk about content creation, PC4, Chilling Reign, and more!

Jake in Cali, Nick in PA, and joined by Zach Lesage in Canada?!?! Triple P talks to the PC 2 Champ on the Chilling Reign's meta, and his thoughts on V-Union. We also dive into the PC4 and Team Event. Zach Lesage is a professional Pokemon TCG player that has been on the show before in one of our first bonus episodes after winning Player's Cup 2. We are super happy to have him on again with his hot takes and analysis of the current state of the game. Credits: Creators: Jake Abrams and Nick Yurko the “Duke of Hobbies” Producer: Nick Yurko the “Duke of Hobbies” Editor: Nick Yurko the “Duke of Hobbies” Host(s): Jake Abrams and Nick Yurko the “Duke of Hobbies” Whimsy Watch: Charlie “Bromack” Ligato Guest(s): Zach Lesage Youtube Channel: Link Twitter: @ZachLesagePTCG Artwork: Logo - Jake Abrams and Nick Yurko Featured: Zach Lesage's Youtube Logo Songs: Intro/Outro: Friday Fugue by Trevor Garrod Advertisement: Fantasyland by Quincas Moreira Whimsey Watch: Shadowing by Corbyn Kites All songs are from the Youtube Audio Library. Websites: Stream: twitch.tv/pittsburghpokemonpodcast Twitter: @PittPokePod YouTube: Pittsburgh Pokemon Podcast Facebook: Pittsburgh Pokemon Podcast Join the Discord Server: https://discord.gg/kEKGgZ8

The Oncology Journal Club - Delivering Oncology News DifferentlyThe Oncology Podcast, brought to you by Oncology News Australia, is proud to present Episode 35 in our series The Oncology Journal Club.Do you think sugary drinks could be responsible for the increased rates of colorectal cancer that we are seeing in young adults? Join us this week as Hans Prenen finds out.Craig Underhill talks us through the TITAN Study and the problem of not acknowledging investigators. And Eva Segelov talks to Kristi Milley about getting meaningful engagement from consumers.You'll also hear the amazing article of the week on Chernobyl and climate change, the final installment of Eva's Manifesto for Good Trial Design and some wonderfully quirky quick bites too.Just a quick note – Eva started her analysis of Bishal Gyawali's paper in Episode 33 and  Episode 34. With the usual top quality banter, papers you won't hear of anywhere else and expert analysis from our Hosts, you are in for another great episode of The Oncology Journal Club!Full bios and the list of all papers discussed are available on our website.For the latest oncology news visit www.oncologynews.com.au and for regular oncology updates for healthcare professionals, subscribe for free to get the weekly The Oncology Newsletter.The Oncology Podcast - An Australian Oncology Perspective

Celio's Network joins us for a discussion on PTCG, life, content creation, and more! JW and Luke team up for this week's episode and even give you a breakdown of what to expect in PC4.

Carter and Carl have a ton of stuff on their minds this week. From the VGC twitter drama, to the Rumble data, to prepping for PC4 in a few weeks, Carter and Carl are here to support you all! Go check out Arcadex, it's the tool we use for all our data gathering and calculations --- https://arcadex.io If you want to support what we do now that our ad cents are gone, you can! Use this link to help us grow! --- https://anchor.fm/littlerootlessons/support Follow us on Twitter --- https://twitter.com/LRLessons | https://twitter.com/MuzikalVGC | https://twitter.com/Mrmissouri25 You can also find us on Twitch! --- https://twitch.tv/Muzikal | https://twitch.tv/MrMissouri25 For other inquiries, feel free to contact us at littlerootlessons@gmail.com Join the Littleroot Lessons Discord! --- https://discord.gg/8tvrcgRcTT Intro | GlitchxCity | Pokemon Diamond and Pearl: Legendary Battle Remix | https://www.youtube.com/watch?v=GM-9R44lMxg Outro | GlitchxCity | Pokemon Diamond and Pearl: Route 225 Lofi Remix | https://www.youtube.com/watch?v=CfAP_Al0uTg --- Support this podcast: https://anchor.fm/littlerootlessons/support

Joe Bernard hops on to talk about his "Life is Just a Game" Invitational win, casting PC3, and much more. The boys pay respects the the Hegster tournament series, talk upcoming PC4, and more! Follow OmniPoke https://twitter.com/OmniPoke https://www.youtube.com/channel/UC9k2vZA_jd83-4gMzRupYrQ Glimwood Challenge: https://play.limitlesstcg.com/tournament/6046bab378bab44bc84b5416/details Follow FlowTKast: https://linktr.ee/flowtkast Support FlowTK: https://flowtkallday.com Follow Aaron: https://twitter.com/dragonownz4 --- Support this podcast: https://anchor.fm/flowtkast/support

Hello everyone! and welcome back to some more Littleroot Lessons! Today, the guys look over their PC4 teams that they used this past weekend, and they talk about the end of a bunch of community events and the start of a big new event they have been working on for a while! Make sure to check out Arcadex, it's the tool we use for all our data gathering and calculations --- https://arcadex.io If you want to support what we do now that our ad cents are gone, you can! Use this link to help us grow! --- https://anchor.fm/littlerootlessons/support Follow us on Twitter --- https://twitter.com/LRLessons | https://twitter.com/Muzikal_33 | https://twitter.com/Mrmissouri25 You can also find us on Twitch! --- https://twitch.tv/Muzikal | https://twitch.tv/MrMissouri25 For other inquiries, feel free to contact us at littlerootlessons@gmail.com Join the Littleroot Lessons Discord! --- https://discord.gg/DY6KzcF Intro | GlitchxCity | Pokemon Diamond and Pearl: Legendary Battle Remix | https://www.youtube.com/watch?v=GM-9R44lMxg Outro | GlitchxCity | Pokemon Diamond and Pearl: Route 225 Lofi Remix | https://www.youtube.com/watch?v=CfAP_Al0uTg --- Support this podcast: https://anchor.fm/littlerootlessons/support

Hello everyone! and welcome back to Littleroot Lessons! Today, Carter and Carl got a bunch of data from the Double Elimination stage of the North America Region, and the teams that made it into the Global Qualifiers for PC3, all so you can be prepared for PC4! Check out Nimbasa City Post on twitter! --- https://twitter.com/NimbasaCityPost Make sure to check out Arcadex, it's the tool we use for all our data gathering and calculations --- https://arcadex.io If you want to support what we do now that our ad cents are gone, you can! Use this link to help us grow! --- https://anchor.fm/littlerootlessons/support Follow us on Twitter --- https://twitter.com/LRLessons | https://twitter.com/Muzikal_33 | https://twitter.com/Mrmissouri25 You can also find us on Twitch! --- https://twitch.tv/Muzikal | https://twitch.tv/MrMissouri25 For other inquiries, feel free to contact us at littlerootlessons@gmail.com Join the Littleroot Lessons Discord! --- https://discord.gg/lrlessons Intro | GlitchxCity | Pokemon Diamond and Pearl: Legendary Battle Remix | https://www.youtube.com/watch?v=GM-9R44lMxg Outro | GlitchxCity | Pokemon Diamond and Pearl: Route 225 Lofi Remix | https://www.youtube.com/watch?v=CfAP_Al0uTg --- Support this podcast: https://anchor.fm/littlerootlessons/support

In this episode of Nursing Australia, hosted by Suzanne Blackeby, we hear: - The latest Nursing Australia News (01:25)The rollout begins, a nurse steps in during a COVID vaccine overdosing error, the WHO warn of ‘long COVID' and The Royal Commission into Aged Care final report handed down. - APNA's campaign for MBS items (03:47)The Australian COVID-19 Vaccination program is up and running with thousands of Australians expected to be vaccinated in the coming weeks, including residents in Aged Care.  While this implementation has been rushed, it's great to have it underway and we wish all aged care nurses well as they take the lead.A major concern for general practices nurses has been the Medicare Benefits Scheme items for the rollout. APNA understands that not providing a way for a nurse to bill for their time to give the COVID vaccines would restrict Australia's 14,500 practice nurses from playing the fullest role they can in the rollout. In the last few days and after significant engagement with government, APNA welcomes the removal of some of the barriers that would have constrained practice nurses.MBS factsheet: http://www.mbsonline.gov.au/internet/mbsonline/publishing.nsf/Content/CC1972BC986AAC8ECA25867D00078BDD/$File/factsheet-vacsmar21.pdf APNA COVID webpage: https://apna.asn.au/hub/news/coronavirusinfofornurses  - APNA Insurance – March special: https://apna.asn.au/membership/insurance  - Vaccine hesitancy and what to do (10:15)To ensure nurses are up to date with the latest on the COVID vaccine rollout, APNA are hosting a special series of live webinars. The recordings of these are available here on Nursing Australia. To view the slides for the webinars, listen to Nursing Australia via the APNA website or if you are listening to audio only via a podcast listening app. In the third webinar Julie Leask took us through Vaccine hesitancy and what to do. Julie is professor and social scientist at the University of Sydney. She studies what people think and do about it, programs and policies. She has a background in public health and prior to that in nursing and midwifery. She is visiting fellow at the National Centre for Immunisation Research and Surveillance and advisor to the World Health Organization.Webinar slides: https://apna.asn.au/files/DAM/1%20Education/Vaccine%20hesitancy%20and%20what%20to%20do%20-%20Slides.pdf Subscribe to The Connect: https://apna.asn.au/workflows/subscribe  - Cancer in primary health care (01:01:26)Primary health care is playing an increasingly important role throughout a patient's cancer journey, from prevention through to palliative care. We're joined by Kristy Millie, the national manager of the primary care collaborative cancer clinical trials group, PC4. She's also the host of ‘Research Roundup', a podcast for people interested in cancer in primary care. Listen to ‘Research Roundup': http://pc4tg.com.au/tag/podcast-research-roundup - Nurse wellness with Dr Avi: Engagement (01:04:27)For our nurse wellness segment in this episode Dr Avi is back to speak about nurse wellbeing, resilience and self-care and ultimately self-leadership.

Look out …it’s a HOST SWAP! Previous guest, and former host of PC4’s other podcast ‘Cheers with Peers’; Sibel Saya took reign of the microphone in this episode of Research Round-up to interview our own PC4 National Manager Dr Kristi Milley. Kristi slid into the guest spotlight to answer questions about her most recent paper entitled “Look who's talking now: Cancer in primary care on Twitter. An observational study“. Kristi’s paper was published in BJGP Open. Kristi and Sibel discuss how often health care professionals and researchers are in their own little ‘twitter bubble’ and the importance of collectively thinking about how they approach engaging with consumers using social media. Kristi went on to highlight how the complexity of cancer diagnosis and role of primary care can regularly be lost in the 280 characters of a tweet. She hopes the results of this study open the door to investigating the role of twitter and other platforms in connecting health care professionals and researchers with the public and in turn the public with research, as well as the message of the importance of primary care right across the cancer continuum.

This month the girls talk to Kristi, a scientific researcher. Researchers make and question information from investigations, experiments, and trials. She also is the National Manager of PC4 a trials group that helps researchers get funding for cancer research. The girls ask about how she works out questions to research. What do researchers do? What's the weirdest thing she's ever researched? Show notes here www.kidsverse.com.au/kidsverse-researcher

Dr Kristi Milley spoke to Dr Karolina Lisy a senior research fellow at Peter MacCallum Cancer Centre and the Australian Cancer Survivorship Centre as well as a member of PC4’s Early Career Research Network. Karolina recently published a paper in the Journal of Clinical Medicine entitled “Sharing Cancer Survivorship Care between Oncology and Primary Care Providers: A Qualitative Study of Health Care Professionals’ Experiences” Karolina emphasised current care for cancer survivors is falling short. We need to shift the model of care and ensure primary care is well represented within this model. Karolina highlighted that patients who receive follow up care from their GP are more satisfied, this form of care is often more convenient, cheaper for the health care system and addresses survivorship needs beyond surveillance. Overall, it is a more holistic approach to care.  Moving forward, Karolina hopes that this study will provide guidance on the best way forward. Show notes are located here : http://pc4tg.com.au/research-round-up-november-2020-with-karolina-lisy/

Welcome to episode 02 of Dear Downloads. Conor and Luke discuss the ends and outs of building PCs, new PS5 info and other tidbits. Looking at building an affordable PC or an overclocked to the max PC, take a listen we discuss our trials and tribulations of creating our own PC setups. ------ show notes ------ been watching archer Evangelion 3+1 releasing in japan 1.32.2021 - when do you think it w ill hit the USA? According to Sony, the PS5 is approximately 390mm (15.4 inches) tall, 260mm (10.24 inches) deep and 104mm (4.09 inches) wide PlayStation NOW $9.99 a month AND on PC, compete with game pass. And include Exclusives, playstations like oh okay hold my beer. PS v8.00 update??? Look at current ps4 changes to broadcast what ps5 experience will be like.., LR I think ps is going to talk about UI on 10/15 FYI https://youtu.be/7TBPrYJDoDE Ps 5 voice chat info & UI https://blog.playstation.com/2020/10/16/details-on-new-voice-chat-functionality-coming-to-ps5/ You ever play destiny? Question from Sam @TheHailst0rm on Twitter “will you branch out to other fields of discussion, like PC builds, and specs, etc.?... Just general guidelines when building your first gaming rig when you're on a budget (where to save money, etc.)” affordable PC build based on alpha gaming YouTube page but why I altered it and based on my discussions with elgato / CORSAIR Problems with OG computers and why did i rebuild Affordable PC build by alpha gaming / modified = $615 total (does not include case / harddrive / operating system / monitor / keyboard / mouse) PCS can be $$$ $25 Thermaltake SMART 600W ATX 12V V2.3/EPS 12V 80 PLUS Certified Active PFC Power Supply PS-SPD-0600NPCWUS-W $165 AMD Ryzen 5 3600 6-Core, 12-Thread Unlocked Desktop Processor with Wraith Stealth Cooler $130 Asus TUF B450M-Plus Gaming AMD Ryzen 2 AM4 DDR4 HDMI DVI-D M.2 mATX Motherboard $230 GeForce GTX 1660 OC 6G Graphics Card, 2X Windforce Fans, 6GB 192-Bit GDDR5, Video Card $65 SOLD OUT Team T-FORCE VULCAN Z 16GB (2 x 8GB) 288-Pin DDR4 SDRAM DDR4 3000 (PC4 24000) Intel XMP 2.0 Desktop Memory Model TLZGD416G3000HC16CDC01 The one unknown is the big navi announcement on 10.28 - what will AMD bring to the ray tracing table vs Nvidia - we some idea based off of what xbox and PS5 graphics but obviously the decrete G cards will provide improvements based off of the SoCs ------ thank you ------ If you have any questions or any thoughts for a new episode topic or game/tech related things we can talk about… you can write us on twitter @deardownloads, thank you all for listening and we will see you next week, buh bye…

Dr Kristi Milley spoke to Dr Rebecca Bergin research fellow at the Cancer Council Victoria and member of PC4’s Early Researcher Career Network. Rebecca recently published a paper in the journal of Cancer Policy entitled “Optimal care pathways: A national policy to improve quality of cancer care and address inequalities in cancer outcomes?" Rebecca highlights that the OCPs specify the key features of cancer care across the whole pathway - from prevention to end of life.  Rebecca emphasises that primary care is an integral part of the OCPs and explains how they have had a unifying effect in Australia in establishing the best cancer care that all people should receive.

Dr Kristi Milley spoke to Sibel Saya who is a Genetic Counsellor and PhD student at University of Melbourne as well as a past host of PC4’s other podcast Cheers with Peers.  Sibel recently published a paper in Public Health Genomics entitled “A Genomic Test for Colorectal Cancer Risk: Is This Acceptable and Feasible in Primary Care?”  Genomic tests can predict risk and tailor screening recommendations for colorectal cancer and primary care could be suitable for their widespread implementation.

Hello listeners of Technically Unbothered, This is my ninth episode for my podcast. The experience of writing the script, recording, and editing were fun! Thank you for taking the time to listen to it and I hope you learned something new from it. If you wish to chat with me and other tech nerds please join The Technically Unbothered Facebook group. Have a Technically Unbothered day! Parts talked about in this episode I7-4790k https://ark.intel.com/content/www/us/en/ark/products/80807/intel-core-i7-4790k-processor-8m-cache-up-to-4-40-ghz.html Fx 9590 https://www.amd.com/en/products/cpu/fx-9590 Gtx 1070 https://www.ebay.com/itm/EVGA-Geforce-GTX-1070-SC-Superclocked-8GB-Graphics-Card/223709998818?epid=28026511535&hash=item341627aee2:g:97gAAOSwms1ecVnb Rx 580 8gb https://www.ebay.com/itm/PowerColor-RED-DEVIL-Radeon-RX-580-DirectX12-AXRX-580-8GBD5-3DH-OC-8GB-256-Bit/324102817647?hash=item4b7608876f:g:SgUAAOSw13hebDOt G skill trident z ram https://www.ebay.com/itm/G-SKILL-TridentZ-RGB-Series-16GB-2-x-8GB-288-Pin-DDR4-SDRAM-DDR4-3600-PC4-288/302871228796?epid=6025937185&hash=item468488317c:g:BY0AAOSwE71c8keD Corsair rgb vengeance pro https://www.amazon.com/CORSAIR-Vengeance-3200MHz-Desktop-Memory/dp/B07D1XCKWW Ryzen 5 1600 af https://www.amazon.com/AMD-Processor-Wraith-Stealth-Cooler/dp/B07XTQZJ28/ref=sr_1_2?dchild=1&keywords=ryzen+5+1600+af&qid=1585324865&s=electronics&sr=1-2

As an amateur competing against professionals, DAC Assistant Bowling Manager Perry Crowell IV, aka PC4, delivered a stunning performance at the U.S. Open during a national TV broadcast in February 2020. DAC members and longtime bowlers Harry Brookes and Mark Stackpoole offer color commentary and Club bowling history. Perry's strike songs:Hip Hop Hooray by Naughty by Nature I Want to Dance with Somebody by Whitney HoustonPerry's bowling balls are made by Hammer Bowling.Perry is sponsored by Vise Inserts and Apparel EFX.

This week we review a recent report of the PC4 group assessing the impact of inter hospital open communication and collaboration on surgical outcomes. In what manner did participation in this collaborative CICU registry improve outcomes for different hospital surgical outcomes? Why was the improvement not homogeneous between centers? How long did it take for the impact of collaboration to positively improve outcomes? We discuss these and other questions with first author Dr. Michael Gaies, Associate Professor of Pediatrics - University of Michigan. doi: 10.1016/j.jacc.2019.09.04

This week we review a recent report of the PC4 group assessing the impact of inter hospital open communication and collaboration on surgical outcomes. In what manner did participation in this collaborative CICU registry improve outcomes for different hospital surgical outcomes? Why was the improvement not homogeneous between centers? How long did it take for the impact of collaboration to positively improve outcomes? We discuss these and other questions with first author Dr. Michael Gaies, Associate Professor of Pediatrics - University of Michigan. doi: 10.1016/j.jacc.2019.09.04

It's our 10th anniversary! So we've decided to release a Bonus Research Round-up to celebrate our 10th anniversary. Dr. Kristi Milley and Sophie Chima talk to Professor Jon Emery, Herman Professor of Primary Care Cancer Research at the University of Melbourne and Director of PC4 and Emily Habgood about the recent publication ‘The Chest Australia Trial: a randomised controlled trial of an intervention to increase consultation rates in smokers at risk of lung cancer’. Lung cancer has one of the lowest survival outcomes of any cancer because of the high occurrence of late-stage diagnosis; with stigma often holding patients back from seeking consultations. Professor Emery was the lead researcher for the CHEST Australia trial, which aimed to reduce lung cancer death through increased early detection. Show notes are available here http://pc4tg.com.au/research-round-up-february-2019-bonus-episode-jon-emery-emily-habgood/

An integral feature of being an Early Career Researchers (ECR) is where to access support. Recognising your community, peers, organisations, all are which are devised to support ECRs. Research can be a lonesome endeavour and encouraging and engaging in support mechanisms is crucial to success. In this episode, Sibel Saya and National Manager of PC4, Dr Kristi Milley, discuss how to most effectively engage in support networks. She also examines new ways of engaging with the research community via social media platforms like Twitter. Show notes are available here http://pc4tg.com.au/cheers-with-peers-episode-5-kristi-milley-and-sibel-saya/

The Research Round-up team interview Associate Professor of General Practice, Joel Rhee from the University of Wollongong. Joel Rhee is Chair of Specific Interest Network of Cancer and Palliative Care of the Royal Australian College of General Practitioners (RACGP) and PC4’s Clinical Oncology Society of Australia representative. In this episode we discuss Associate Professor Rhee’s recent BMJ publication General practitioners (GPs) and end-of-life care: a qualitative study of Australian GPs and specialist palliative care clinicians. GPs are well placed to be included in end-of-life care for patients with life-limiting illnesses, and yet GPs have different understandings of their role. Joel discusses the important barriers and facilitators to GP involvement in end-of-life care; and why the Australian health care system needs to better support GPs. Show notes are located here http://pc4tg.com.au/research-round-up-january-2019-joel-rhee/

PC4 is a small protein with unique DNA-binding properties that affects transcription and has presumptive roles in DNA repair and genome stability. It was originally isolated from a cofactor fraction termed the ‘‘upstream stimulatory activity’’ (USA) of HeLa cell nuclear extracts. The cofactor has been shown to broadly enhance RNA polymerase II-driven gene transcription in the presence of activators (e.g., hormone receptors, viral activators, cell-specific and ubiquitous activators). Although such data imply that PC4 is a very important factor in vivo, human tumor cell lines with PC4 knockdowns are without obvious phenotypes. To further study the in vivo role of PC4, we constructed constitutive and conditional knockout mouse models as well as knockout embryonic stem cells. Mammalian PC4 is here shown to be an essential factor during early embryogenesis. PC4-/- embryos develop normally until E5.5, but then degenerated around E7.5. PC4 knockout ES cell lines were generated from PC4-/- blastocysts (E3.5), which develop normally from 2-cell stage embryos. All PC4 knockout ES cell lines displayed a severe proliferation deficit phenotype, which could be partially rescued by re-expression of human PC4. The reduced proliferation was not due to an increase in cell apoptosis. Occasionally, PC4 knockout ES cells undergo tetraploidy apparently as a survival mechanism to circumvent the loss of PC4. Knocking down PC4 in mouse embryonic fibroblasts also resulted in reduced proliferation rates. These data indicate that PC4 is important for cell proliferation in embryos. Moreover, in vitro embryoid body formation and in vivo teratoma formation assays provided preliminary evidence for an important role of PC4 in differentiation. Differentiated ES cells displayed alterations in germ-layer specific gene expression, that are in agreement with morphological abnormalities observed in histological analyses of PC4-/- embryos at E6.5 and E7.5. Thus, depletion of PC4 results in reduced proliferation and impaired differentiation, the consequence of which appears to be of gastrulation arrest in early embryos. In an attempt to understand the underlying mechanisms of this phenotype, differential gene expression in ES knockout and wild-type cells was studied. Microarray and qRT-PCR analyses revealed more than 2 fold alterations in expression of many genes in knockout ES cells as compared with wild-type cells. These include enhanced expression of p21, Rb1, and Ddit4l, and lower expression of Sfmbt2, Tdrd12, and Dppa3, suggesting a specific direct or indirect physiological role of the cofactor. Nevertheless, the previously proposed role of PC4 in p53 expression and function was not confirmed using the knockout model. Taken together, this work represents the first description of the physiological functions of PC4 during mammalian embryogenesis.

DNA lesions arising from environmental and endogenous sources induce various cellular responses including cell cycle arrest, DNA repair and apoptosis. Although detailed insights into the biochemical mechanisms and composition of DNA repair pathways have been obtained from in vitro experiments, a better understanding of the interplay and regulation of these pathways requires DNA repair studies in living cells. In this study we employed laser microirradiation and photobleaching techniques in combination with specific mutants and inhibitors to analyze the real-time accumulation of proteins at laser-induced DNA damage sites in vivo, thus unravelling the mechanisms underlying the coordination of DNA repair in living cells. The immediate and faithful recognition of DNA lesions is central to cellular survival, but how these lesions are detected within the context of chromatin is still unclear. In vitro data indicated that the DNA-damage dependent poly(ADP-ribose) polymerases, PARP-1 and PARP-2, are involved in this crucial step of DNA repair. With specific inhibitors, mutations and photobleaching analysis we could reveal a complex feedback regulated mechanism for the recruitment of the DNA damage sensor PARP-1 to microirradiated sites. Activation of PARP-1 results in localized poly(ADP-ribosyl)ation and amplifies a signal for the subsequent rapid recruitment of the loading platform XRCC1 which coordinates the assembly of the repair machinery. Using similar techniques we could demonstrate the immediate and transient binding of the RNA Polymerase II cofactor PC4 to DNA damage sites, which depended on its single strand binding capacity. This establishes an interesting link between DNA repair and transcription. We propose a role for PC4 in the early steps of the DNA damage response, recognizing and stabilizing single stranded DNA (ssDNA) and thereby facilitating DNA repair by enabling repair factors to access their substrates. After DNA lesions have been successfully detected they have to be handed over to the repair machinery which restores genome integrity. Efficient repair requires the coordinated recruitment of multiple enzyme activities which is believed to be controlled by central loading platforms. As laser microirradiation induces a variety of different DNA lesions we could directly compare the recruitment kinetics of the two loading platforms PCNA and XRCC1 which are involved in different repair pathways side by side. We could demonstrate that PCNA and XRCC1 show distinct recruitment and binding kinetics with the immediate and fast recruitment of XRCC1 preceding the slow and continuous recruitment of PCNA. Introducing consecutively multiple DNA lesions within a single cell, we further demonstrated that these different recruitment and binding characteristics have functional consequences for the capacity of PCNA and XRCC1 to respond to successive DNA damage events. To further study the role of PCNA and XRCC1 as loading platforms in DNA repair, we extended our analysis to their respective interaction partners DNA Ligase I and III. Although these DNA Ligases are highly homologous and catalyze the same enzymatic reaction, they are not interchangeable and fulfil unique functions in DNA replication and repair. With deletion and mutational analysis we could identify domains mediating the specific recruitment of DNA Ligase I and III to distinct repair pathways through their interaction with PCNA and XRCC1. We conclude that this specific targeting may have evolved to accommodate the particular requirements of different repair pathways (single nucleotide replacement vs. synthesis of short stretches of DNA) and thus enhances the efficiency of DNA repair. Interestingly, we found that other PCNA-interacting proteins exhibit recruitment kinetics similar to DNA Ligase I, indicating that PCNA not only serves as a central loading platform during DNA replication, but also coordinates the recruitment of multiple enzyme activities to DNA repair sites. Accordingly, we found that the maintenance methyltransferase DNMT1, which is known to associate with replication sites through binding to PCNA, is likewise recruited to DNA repair sites by PCNA. We propose that DNMT1, like in DNA replication, preserves methylation patterns in the newly synthesized DNA, thus contributing to the restoration of epigenetic information in DNA repair. In summary, we found immediate and transient binding of repair factors involved in DNA damage detection and signalling, while repair factors involved in the later steps of DNA repair, like damage processing, DNA ligation and restoration of epigenetic information, showed a slow and persistent accumulation at DNA damage sites. We conclude that DNA repair is not mediated by binding of a preassembled repair machinery, but rather coordinated by the sequential recruitment of specific repair factors to DNA damage sites.