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Latest podcast episodes about these dna

Dark Side of Wikipedia | True Crime & Dark History
Full Interview With Suspected Gilgo Beach Killer Rex Heuermann

Dark Side of Wikipedia | True Crime & Dark History

Play Episode Listen Later Jul 19, 2023 20:34


Rex Heuermann, a former public official, faces serious charges linked to the unsolved Long Island Serial Killer case. The charges were brought forward due to an exhaustive investigation that utilized advanced forensic techniques and leveraged digital footprints Heuermann left behind.   The initial link to Heuermann was made through comprehensive cell tower data analysis, connecting him to calls made to victims' families using what has been referred to as the Barthelemy Phone. Suspicious activities on this phone, including calls to victims' families and unusual cessation of activity, coincided with Heuermann's travel schedule.   Further investigation revealed Heuermann's illicit use of burner phones and online accounts under fictitious names. These were primarily used to engage with sex workers and conduct disturbing searches related to sadistic, torture-related, and child pornography. Additionally, these burner accounts were used to track developments in the investigation into the serial murders, revealing Heuermann's attempt to stay one step ahead of the investigators.   In a significant discovery, a burner email account linked to Heuermann, registered under the pseudonym "Thomas Hawk," was found to have conducted thousands of searches about sex workers, disturbing pornography, and ongoing serial killer investigations.   Heuermann's residence has also been implicated in the investigation. Forensic investigations revealed that DNA samples from his residence matched those found on female hairs on three of the victims, effectively linking the crimes directly to his household. These DNA profiles were matched with a high degree of certainty, excluding most of the North American population.   Another damning piece of evidence was the recovery of a male hair from one of the victim's burlap wrappings. Advanced DNA analysis matched this hair to Heuermann, further solidifying his involvement in the crimes.   The evidence indicates that Heuermann not only had direct contact with the victims but also went to great lengths to evade detection, including using burner phones, fake online accounts, and careful monitoring of the ongoing investigation.   Rex Heuermann is in custody, facing multiple charges related to the Long Island Serial Killer case. If convicted, he will likely spend the rest of his life in prison without the possibility of parole. As the case unfolds, it serves as a stark reminder of the cruel capacity of individuals and the vital importance of ongoing advancements in forensic and investigative techniques. Want to listen to ALL our Podcasts AD-FREE? Subscribe through APPLE PODCASTS, and try it for 3 days free: https://tinyurl.com/ycw626tj Follow Our Other Cases: https://www.truecrimetodaypod.com The latest on Chad & Lori Daybell, The Murder of Ana Walshe, Alex Murdaugh, Bryan Kohberger, Lucy Letby, Kouri Richins, Justice for Harmony Montgomery, The Murder of Stephen Smith, The Murder of Madeline Kingsbury, and much more! Listen at https://www.truecrimetodaypod.com

Catching the Long Island Serial Killer
5: Full Interview With Suspected Gilgo Beach Killer Rex Heuermann

Catching the Long Island Serial Killer

Play Episode Listen Later Jul 19, 2023 20:34


Rex Heuermann, a former public official, faces serious charges linked to the unsolved Long Island Serial Killer case. The charges were brought forward due to an exhaustive investigation that utilized advanced forensic techniques and leveraged digital footprints Heuermann left behind.   The initial link to Heuermann was made through comprehensive cell tower data analysis, connecting him to calls made to victims' families using what has been referred to as the Barthelemy Phone. Suspicious activities on this phone, including calls to victims' families and unusual cessation of activity, coincided with Heuermann's travel schedule.   Further investigation revealed Heuermann's illicit use of burner phones and online accounts under fictitious names. These were primarily used to engage with sex workers and conduct disturbing searches related to sadistic, torture-related, and child pornography. Additionally, these burner accounts were used to track developments in the investigation into the serial murders, revealing Heuermann's attempt to stay one step ahead of the investigators.   In a significant discovery, a burner email account linked to Heuermann, registered under the pseudonym "Thomas Hawk," was found to have conducted thousands of searches about sex workers, disturbing pornography, and ongoing serial killer investigations.   Heuermann's residence has also been implicated in the investigation. Forensic investigations revealed that DNA samples from his residence matched those found on female hairs on three of the victims, effectively linking the crimes directly to his household. These DNA profiles were matched with a high degree of certainty, excluding most of the North American population.   Another damning piece of evidence was the recovery of a male hair from one of the victim's burlap wrappings. Advanced DNA analysis matched this hair to Heuermann, further solidifying his involvement in the crimes.   The evidence indicates that Heuermann not only had direct contact with the victims but also went to great lengths to evade detection, including using burner phones, fake online accounts, and careful monitoring of the ongoing investigation.   Rex Heuermann is in custody, facing multiple charges related to the Long Island Serial Killer case. If convicted, he will likely spend the rest of his life in prison without the possibility of parole. As the case unfolds, it serves as a stark reminder of the cruel capacity of individuals and the vital importance of ongoing advancements in forensic and investigative techniques. Want to listen to ALL our Podcasts AD-FREE? Subscribe through APPLE PODCASTS, and try it for 3 days free: https://tinyurl.com/ycw626tj Follow Our Other Cases: https://www.truecrimetodaypod.com The latest on Chad & Lori Daybell, The Murder of Ana Walshe, Alex Murdaugh, Bryan Kohberger, Lucy Letby, Kouri Richins, Justice for Harmony Montgomery, The Murder of Stephen Smith, The Murder of Madeline Kingsbury, and much more! Listen at https://www.truecrimetodaypod.com

The Infatu Asian Podcast
Ep 81: Exploring our Ancestry - Looking at Our DNA Test Results

The Infatu Asian Podcast

Play Episode Listen Later Jun 14, 2023 47:01


In honor of Father's Day.. we figured we'd look into our DNA!   I've been wanting to take a DNA test since they came out, so I figured it'd be fun to do it on the air while we record.  These DNA kits can help fill in the picture of where your ancestors came from.  Two friends and I ordered a kit from Ancestry.com  We got our results and *tried* to wait until this recording before taking a look.  It was definitely fun, and in some cases VERY interesting! I'm not sponsored by them, but Ancestry.com is having a sale right now, and you can get a kit for $59 if you order before June 18th, 2023!  (They're normally $99, but they go on sale around Black Friday too) Special thanks to Alan and Sarika, who agreed to take the tests and reveal their results with me on the air.   As I always mention, you can write to us at: infatuasianpodcast@gmail.com, and please follow us on Instagram and Facebook @theinfatuasianpodcast  Please follow us wherever you get your podcasts.  We could use your ratings and reviews over at Apple. And now Spotify! Our Theme: “Super Happy J-Pop Fun-Time” by Prismic Studios was arranged and performed by All Arms Around  #ancestry #dnatest #23andme #bioinformatics #asianpodcast #asian #asianamerican #infatuasian #infatuasianpodcast #aapi #veryasian  #asianamericanpodcaster #asianauthor #representationmatters  

Passion Struck with John R. Miles
Dr. Kara Fitzgerald on How to Become a Younger You by Reversing Your Biological Age EP 174

Passion Struck with John R. Miles

Play Episode Listen Later Aug 11, 2022 61:01 Transcription Available


In today's episode of Passion Struck with John R. Miles, I interview Dr. Kara Fitzgerald about her groundbreaking work in understanding how you can reverse your biological age and become a younger you. | Brought to you by Gusto. Go to (https://www.gusto.com/passionstruck)  for three months free. Dr. Kara Fitzgerald, ND, IFMCP, is the first-ever recipient of the Emerging Leadership Award from the Personalized Lifestyle Medicine Institute in recognition of her work on DNA methylation. Dr. Fitzgerald directs a large, integrative in-person and virtual clinical practice in Connecticut. She is engaged in clinical research on the DNA methylome using a diet and lifestyle intervention developed in her clinic. Her first study was published in the journal Aging. Earlier this year, she published Younger You: Reduce Your Bio Age and Live Longer, Better, an application-based program based on the study. * Purchase Younger You: https://amzn.to/3BZwFiP  (Amazon Link) --► Get the full show notes: https://passionstruck.com/dr-kara-fitzgerald-become-younger-you/  --► Subscribe to My Channel Here: https://www.youtube.com/c/JohnRMiles --► Subscribe to the podcast: https://podcasts.apple.com/us/podcast/passion-struck-with-john-r-miles/id1553279283 *Our Patreon Page: https://www.patreon.com/passionstruck. Thank You to Our Sponsors This episode of Passion Struck with John R. Miles is brought to you by Gusto, which provides cloud-based payroll, benefits, and human resource management software for businesses based in the United States. For three months free, go to https://www.gusto.com/passionstruck. What I Discuss With Dr. Kara Fitzgerald About How You Reverse Your Biological Age Through Lifestyle and Diet Choices Dr. Fitzgerald and I discuss how there is a link between aging and chronic disease, and it has everything to do with epigenetics and their effect on aging. Biological age looks at specific patterns of a key epigenetic mark called DNA methylation. These DNA methylation patterns can reliably predict how fast our body is aging. Methylation is as essential to our bodies as breathing and is happening in our bodies and within our cells all of the time. Therefore, if you can give yourself the DNA methylations of a younger you, you can reset your biological aging process. She provides key ways to do this throughout our interview that are based on her years of experience backed by clinical trials.  Why do most adults spend the last 16 years of their lives suffering from chronic conditions and taking multiple medications? Why reversing biological age is key. Why it's epigenetics and not your genetics that determines your level of health and age How is epigenetic expression greatly influenced by DNA methylation? How do our diet and lifestyle choices impact DNA methylation? The results of her research and a groundbreaking clinical trial showed a reduction of biological age by three years. Why it is our daily choices and routines that add years to your life or reduce them Her diet and lifestyle program which increases healthspan and reduces your biological age. And so much more. Where to Find Dr. Kara Fitzgerald: * Website: https://www.drkarafitzgerald.com/  * LinkedIn: https://www.linkedin.com/in/kara-n-fitzgerald-b77265b/  * Instagram: https://www.instagram.com/drkarafitzgerald/  * Twitter: https://twitter.com/kfitzgeraldnd  * Facebook: https://www.facebook.com/DrKaraFitzgerald/  Show Links * My solo episode on how to reverse aging: https://passionstruck.com/the-reverse-aging-effect-stay-young-and-healthy/  * My solo episode on how to heal from the consequences of abuse: https://passionstruck.com/heal-from-the-shattering-consequences-of-abuse/  In my interview with Colin O'Brady on his new book The 12-Hour Walk, we discuss how to conquer your mind: https://passionstruck.com/colin-obrady-12-hour-walk-transform-your-life/  * My interview with Air Force Veteran, Entrepreneur, and Speaker D.J. Vanas about unleashing the warrior within: https://passionstruck.com/dj-vanas-on-unleashing-your-warrior-within/  * My interview with screenwriter and director Abi Morgan on the power of Hope in our lives: https://passionstruck.com/abi-morgan-on-the-importance-of-hope/  * My interview with Kara Robinson Chamberlain on how she escaped from a kidnapp ning by a serial killer: https://passionstruck.com/kara-robinson-chamberlain-be-vigilant/  * My interview with Jean Oelwang on the power of partnerships: https://passionstruck.com/jean-oelwang-what-will-you-love-into-being/  * My interview with Katy Milkman, Ph.D. on how to create lasting behavior change: https://passionstruck.com/katy-milkman-behavior-change-for-good/  * My solo episode on why micro choices matter: https://passionstruck.com/why-your-micro-choices-determine-your-life/ * My solo episode on why you must feel to heal: https://passionstruck.com/why-you-must-feel-to-find-emotional-healing/   -- John R. Miles is the CEO, and Founder of PASSION STRUCK®, the first of its kind company, focused on impacting real change by teaching people how to live Intentionally. He is on a mission to help people live a no-regrets life that exalts their victories and lets them know they matter in the world. For over two decades, he built his own career applying his research of passion struck leadership, first becoming a Fortune 50 CIO and then a multi-industry CEO. He is the executive producer and host of the top-ranked Passion Struck Podcast, selected as one of the Top 50 most inspirational podcasts in 2022. Learn more about John: https://johnrmiles.com/  ===== FOLLOW JOHN ON THE SOCIALS ===== * Twitter: https://twitter.com/Milesjohnr * Facebook: https://www.facebook.com/johnrmiles.c0m * Medium: https://medium.com/@JohnRMiles​ * Instagram: https://www.instagram.com/john_r_miles * LinkedIn: https://www.linkedin.com/in/milesjohn/ * Blog: https://johnrmiles.com/blog/ * Instagram: https://www.instagram.com/passion_struck_podcast * Gear: https://www.zazzle.com/store/passion_sruck_podcast

Neuroepic: Nature, Nurture, Food, Family, Brains
34. Epigenetics of Drug Addiction in the Midst of the Opioid Crisis

Neuroepic: Nature, Nurture, Food, Family, Brains

Play Episode Listen Later Aug 6, 2022 9:32


Prescription painkillers are vital for proper medical treatment for patients who endure chronic or acute pain. Opioids in particular are commonly prescribed due to their effectiveness in treating pain, however these drugs are known to be highly addictive and can have terrible effects if overused or abused. Opioids such as Vicodin, morphine, and fentanyl are known to be addictive and can lead to severe consequences when mistreated. How these drugs and the addiction it causes affect the brain is not yet fully understood, however every year we learn more and come closer to painting a full picture of the way the brain is affected by these drugs. Recent studies suggest opioid consumption leads to changes in DNA and DNA storage systems that have direct, long-lasting effects on brain circuitry as well as behavior. These DNA modifications, specifically those located in brain cells, have spawned an increasing amount of research which has led to the development of the field of neuroepigenetics. The field of neuroepigenetics looks at these DNA modifications that have been caused by life choices and the environment, and how they affect cell structure and function. These studies will potentially give us a better understanding of how and why opioids cause the effects they do, and hopefully inspire ways in which they can still be used without their detrimental effects.

The Research Like a Pro Genealogy Podcast

Today's episode of Research Like a Pro is about the DNA Standards in the Board for Certification of Genealogist's Genealogy Standards manual. These DNA standards were added in 2019 as part of the second edition of Genealogy Standards. We discuss some applications of the DNA standards to our own work and ideas for how to meet them. Links How to Write and Publish a Proof Argument with DNA Evidence by Nicole at Family Locket Genealogy Research in the 21st Century Part 4: Learning to Use DNA and Standards by Nicole at Family Locket Genealogy Standards - book by the Board for Certification of Genealogists; affiliate link to Amazon (we receive a portion of the proceeds but the price isn't changed) ICAPGen Rubrics: --Level 1 Four-generation Project Self-Assessment --Level 3 Final Project Grading Rubric How to Prepare DNA Research Reports for Sharing by Nicole at Family Locket - includes sample permission forms for publication Informed Consent Agreement and Beneficiary Agreement - by Blaine Bettinger at The Genetic Genealogist Professional Genealogy: Preparation, Practice & Standards - edited by Elizabeth Shown Mills - this is the 2nd edition of ProGen; affiliate link to Amazon (we receive a portion of the proceeds but the price isn't changed) Research Like a Pro eCourse Study Group - more information and email list Research Like a Pro: A Genealogist's Guide by Diana Elder with Nicole Dyer on Amazon.com Thank you Thanks for listening! We hope that you will share your thoughts about our podcast and help us out by doing the following: Share an honest review on iTunes or Stitcher. You can easily write a review with Stitcher, without creating an account. Just scroll to the bottom of the page and click "write a review." You simply provide a nickname and an email address that will not be published. We value your feedback and your ratings really help this podcast reach others. If you leave a review, we will read it on the podcast and answer any questions that you bring up in your review. Thank you! Leave a comment in the comment or question in the comment section below. Share the episode on Twitter, Facebook, or Pinterest. Subscribe on iTunes, Stitcher, Google Play, or your favorite podcast app. Sign up for our newsletter to receive notifications of new episodes. Check out this list of genealogy podcasts from Feedspot: Top 20 Genealogy Podcasts

the bioinformatics chat
#44 DNA tagging and Porcupine with Kathryn Doroschak

the bioinformatics chat

Play Episode Listen Later Apr 29, 2020 45:00


Porcupine is a molecular tagging system—a way to tag physical objects with pieces of DNA called molecular bits, or molbits for short. These DNA tags then can be rapidly sequenced on an Oxford Nanopore MinION device without any need for library preparation. In this episode, Katie Doroschak explains how Porcupine works—how molbits are designed and prepared, and how they are directly recognized by the software without an intermediate basecalling step. Links: Porcupine: Rapid and robust tagging of physical objects using nanopore-orthogonal DNA strands (Kathryn Doroschak, Karen Zhang, Melissa Queen, Aishwarya Mandyam, Karin Strauss, Luis Ceze, Jeff Nivala)

People Behind the Science Podcast - Stories from Scientists about Science, Life, Research, and Science Careers
540: Developing DNA Vaccines and Treatments for Cancer and Other Diseases - Dr. David Weiner

People Behind the Science Podcast - Stories from Scientists about Science, Life, Research, and Science Careers

Play Episode Listen Later Feb 10, 2020 43:34


Dr. David Weiner is Executive Vice President, Director of the Vaccine & Immunotherapy Center, and the W.W. Smith Charitable Trust Professor in Cancer Research at The Wistar Institute. He is also Emeritus Professor of Pathology and Laboratory Medicine at the Perelman School of Medicine at the University of Pennsylvania. In the lab, David and his colleagues are creating drugs using the same DNA codes and signals that our bodies use naturally. The drugs they are creating are natural compounds that can be given to people to prevent them from getting sick or to help them be healthier. One area of David’s research focuses on developing DNA vaccines to prevent illness. These DNA vaccines are designed to create specific proteins that trigger the immune system to respond to fight particular pathogens. David and his wife enjoy reading and going on walks with their dog Ruby. Ruby is a Shih Tzu Yorkie mix that they rescued after a recent hurricane in Puerto Rico. David received his B.S. in biology from the State University of New York at Stony Brook, his M.S. in biology from the University of Cincinnati, and his  Ph.D. in developmental biology from the University of Cincinnati College of Medicine. Afterwards, David worked as a research fellow in the Department of Pathology and Laboratory Medicine at the University of Pennsylvania before joining the faculty there. He held a joint position as Assistant Professor of Pathology and Laboratory Medicine at The Wistar Institute and the University of Pennsylvania School of Medicine from 1990-1993. David returned to The Wistar Institute in 2016 to accept his current positions. Among his many awards and honors, David has been elected as a Fellow of the American Association for the Advancement of Science and the International Society for Vaccines. He has also received the NIH Director’s Transformative Research Award, the Vaccine Industry Excellence Award for Best Academic Research Team, the prestigious Hilleman Lectureship from the Children’s Hospital of Philadelphia, a Stone Family Award from Abramson Cancer Center for his groundbreaking work on DNA vaccines for cancer immune therapy, and the Scientific Achievement Award from Life Sciences Pennsylvania. In addition, David was named a "Top 20 Translational Researchers" in 2016 by Nature Biotechnology.

SIBO SOS™ with Shivan Sarna
008: Why Are You Having These Symptoms & How To Overcome Them w/ Dr. Megan Taylor

SIBO SOS™ with Shivan Sarna

Play Episode Listen Later May 30, 2019 23:59


Finally getting to the route of your SIBO symptoms is usually more than just heading to the doctor for a blood test. There are so many other factors and indicators at play! From DNA tests and gene markers to vitamin deficiencies and dehydration, a little exploration into your health can go a long way. Continuing my conversation with Dr. Megan Taylor, in this episode, we’re talking about how to help manage your SIBO symptoms. Treating SIBO can involve a number of different approaches, starting with, but not limited to, completing one of those ultra-popular DNA tests. These DNA tests not only give you ancestry information, but they’re also full of gene indicators and variants that will help you figure out your current health problems, and insight into whether you’ll be more susceptible to certain conditions in the future. SIBO patients can have a hard time breaking down, digesting, and utilizing the food and drinks we consume. We talk about what the impact of being unable to break down stress neurotransmitters has on our body - as it’s often more subtle than you might think. Vitamins and minerals should be supplemented, for quite a lot of the population, but in particular for SIBO patients. We should be looking at including extra B vitamins and iron into our daily routine. Often, and because of the way SIBO presents itself, there might be a worry of having too many B vitamins in our system - but current research shows that the body is able to expel what extra it has with no impact on the system. SIBO patients might find that they’re chronically thirsty and, even though they drink plenty of water every day, their thirst is real and constant. This is due to a disconnect within the adrenal glands, and Megan recommends using colorful sea salt in water a few times a day to help out sodium levels. Finally, Megan shares how coffee impacts the adrenal glands of SIBO patients and whether or not we should be drinking coffee every day. Current research indicates that, in moderation, coffee is actually good for us… but does that apply when you have SIBO? Do you drink coffee every day? How do you supplement your vitamins and minerals? Have you ever taken a DNA test like 23andme? In This Episode: What gene variants will make you more or less susceptible to certain conditions Why SIBO patients have a hard time breaking down stress neurotransmitters and what impact this has What high B vitamin levels can indicate for SIBO patients What vitamins and supplements you should take when you have SIBO How much water SIBO patients should drink every day Why everyone should be including extra trace minerals in their water What the connection between coffee and your adrenal glands is   Quotes: “With a b12 deficiency, you end up not having as much cell division happening, so you end up with bigger cells because cells haven’t been able to go through the division process.” (9:49) “Many SIBO patients have adrenal and salt balance issues in our bodies. Our adrenal glands are helpful for regulating salt in our blood. And that can contribute to hydration and not being able to hold onto our water.” (16:03) “All the research out there is telling us that coffee in moderation, just like alcohol in moderation, can actually be really helpful. There’s a lot of antioxidants, among other things, in coffee.” (19:13) Links Find Dr. Megan Taylor Online | Facebook Join the SIBO SOS 2019 Speaker Series Learn more about SIBO SOS Online | Facebook Join the SIBO SOS Facebook Community   Check out the full episode post here Keep up with everything SIBO SOS™ here Follow Shivan on Facebook | Twitter | Vimeo Join the SIBO SOS™ Facebook Community Get access to the complete SIBO SOS™ course catalog

The mindbodygreen Podcast
108: You're Probably Vitamin D Deficient & Why It Matters With Steven Gundry, M.D.

The mindbodygreen Podcast

Play Episode Listen Later Mar 25, 2019 68:30


Want to live longer? Us too. Steven Gundry, M.D., is here to tell us how to do just that. As a heart surgeon and best-selling author, and author of the new book The Longevity Paradox: How to Die Young at a Ripe Old Age, Dr. Gundry is doing what it takes to live a long, healthy life. When he was on the mbg podcast last February, he talked about avoiding lectins, grains, and sugar—all tentpoles to his New York Times best-selling book The Plant Paradox: The Hidden Dangers in “Healthy Foods That Cause Disease and Weight Gain. Now, he’s back with even more insight on bettering our health and debunking the many myths of longevity. At the core of our conversation is the microbiome. In his practice in California, Dr. Gundry has been seeing what he calls “superolds” or people living beyond 100 years old. He’s found the common denominator in these individuals is a diverse, healthy microbiome. “It’s actually the microbiome, and its interface with the wall of your gut that will determine what happens to you,” Dr. Gundry shared. While it may seem genetics determine our health and longevity, he pointed out that our genome only plays about 6 percent into what’s going to happen to us and about 94 percent is related to our lifestyle: the food we eat and the people we’re surrounded by. In simple terms, it means we can do something to change the trajectory of our health. On his list for how to live to 100 and beyond is getting enough Vitamin D. “People with the highest vitamin D levels have the longest telomeres,” he explained. These DNA-protein structures protect our chromosomes and the longest ones are the most telling of our longevity. Unfortunately, there’s a high chance you’re not getting enough as 80 percent of Southern Californians are Vitamin D deficient (that says a lot). Dr. Gundry pointed out that it can take 40,000 IU a day of Vitamin D to raise someone’s Vitamin D levels, which he says is vital to our health. This episode will leave you with more knowledge about what’s going on in your gut and inspire you to make changes that could add years on your life. To contact Colleen or Jason with comments, questions, or speaker ideas, please email podcast@mindbodygreen.com. For all sponsorship inquiries, please email sales@mindbodygreen.com. Want to join our podcast email newsletter? Sign up here!

Ben Coomber Radio
#389 – DNA Testing: Science or Snake Oil?

Ben Coomber Radio

Play Episode Listen Later Jan 9, 2019 44:30


Had one of these DNA tests to optimise your diet & fitness? I have. These DNA testing kits are now everywhere and promise big things. To test athletic potential, to optimise diet, training & supplements, and more. But what does the science say, is there legitimacy in the claims? Do we know much about DNA right now, and what our genes say about us? And where does it sit on the ethical spectrum, telling people some of this data…

Chemie und Pharmazie - Open Access LMU - Teil 02/02
Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes. Toward a synthetic virus-like gene-transfer vehicle.

Chemie und Pharmazie - Open Access LMU - Teil 02/02

Play Episode Listen Later Jan 1, 1992


Complexes containing plasmid DNA, transferrin-polylysine conjugates, and polylysine-conjugated peptides derived from the N-terminal sequence of the influenza virus hemagglutinin subunit HA-2 have been used for the transfer of luciferase or beta-galactosidase marker genes to K562 cells, HeLa cells, and BNL CL.2 hepatocytes. These DNA complexes mimic the entry of viruses into cells, as they contain functions for (i) the packaging of the nucleic acid with polylysine, (ii) the attachment to the cell and receptor-mediated endocytosis with transferrin as a ligand, and (iii) the release from endosomes by using membrane-disrupting influenza peptides. The presence of these influenza peptide conjugates in the DNA complexes renders the complexes active in membrane disruption in a liposome leakage assay and results in a substantial augmentation of the transferrin-polylysine-mediated gene transfer.