Podcasts about yops

We apologize for the lack of info, but Soundcloud's description limitations have our hands tied. Also, better late than never on this Podcast Episode

Live Performance by Strawbitty Yops on the Kindie Rock Stars Podcast, featuring songs from their latest album What Does Love look Like: Live Songs Featured:What Does Love Look LikeLet's Get ExcitedLevel UpYouTube Video Link:  https://youtu.be/BWvdtOEqdk8Kindie Rock Stars showcases some of the best children's bands and musicians, with artist stories, song stories, songs and live performances.www.patrickadamsbooks.com/kindierockstarshttps://www.strawbittyyops.com/

I'm so excited to have Strawbitty Yops in studio this weeks on Radio Active Kids! I can't wait to chat with them & have them perform some tunes! We'll play their new song ft. Carrie Ferguson, Lavender Blues, & Ants on a Log, and we also have new songs by Aaron Nigel Smith & Fyütch, Soul Push covering Imagination Movers, The BenAnna Band (ft. Strawbitty Yops, Queer Kid Stuff, Mr. Nick Davio & more), Micah and Me, Martin and Rose, fleaBITE & Levity Beet, Ashley Mills Monaghan (also ft. Music with Michal & uncle dox), Carrie Ferguson, #BisexualHarry, Yam On ft. Marshall Allen of the Sun Ra Arkestra, Paper Bagg Band, Brighter Light Brigade ft. Mista Cookie Jar, & Stuart Stotts!!! Here's the playlist!

This week on Radio Active Kids, I'm so excited to interview the awesome new kindie band Strawbitty Yops! Also, new tunes by Music with Michal, Joanie Leeds & Fyütch, Will's Jams & Seeka Sings, Billy Kelly, Captain Festus McBoyle, Stacey Peasley, Steve Elci & #JillLamoureux on a comp by One Little Finger, The Relative Minors, Little Miss Ann & No Parking Studio, Levity Beet Music, fleaBITE, Key Wilde & Mr Clarke, Shawny, #JamesandtheMaraudersMap on a comp by Wizrocklopedia Compilation Club, Jump for Joy Music, Cee Bee Teatime, & Whoopie Chicken! Here's the playlist!

Live Performance by Strawbitty Yops on the Kindie Rock Stars Podcast, featuring the songs: Live Songs Featured:This PersonThe Mad SongMusic Video Featured:All the ColorsYouTube Video Link:  https://youtu.be/Lg_yBJAgvsYKindie Rock Stars showcases some of the best children's bands and musicians, with artist stories, song stories, songs and live performances.www.patrickadamsbooks.com/kindierockstarshttps://www.strawbittyyops.com/

The audio version of Strawbitty Yops live performance of their songs Winter Magic and Let's Make some music on the Kindie Rock Stars Podcast.Check out the YouTube video of the performance here:https://youtu.be/iw5Sk_fGmRcWebsitewww.strawbittyyops.com

En plática con el Inge presenta: Yops Martínez Ing. en Mecatrónica egresado del TECNM campus Cuautla. Cuenta con varios años de experiencia en la industria como jefe de planos y diseño. Docente de universidad y preparatoria en el estado de Morelos. Tallerista en varios eventos tecnológicos nacionales. CEO de Robot Rumble Mexico, Responsable legal de Robot Rumble Internacional Contest and Meeting Fest. --- Send in a voice message: https://anchor.fm/ing-piedra/message

In today's ep, the Bubble Buds are back to talk about Katie-Ellen's case of the Marchies, Amitai's case of pandemic privilege, and the inherent dorkiness of hobbies. Katie-Ellen explains what Highland games are and Ami cops to his romantic death wish. This week, Katie-Ellen is horny for the new Lola Bunny and Amitai is horny for the YouTube channel Solid Content (https://youtube.com/c/SolidContent). Make sure to get your tickets for SHOW OF GO OFF, Katie's Online Comedy Variety Show on March 13th. Tickets are by donation to Swan Vancouver. Info at https://www.eventbrite.com/e/show-off-go-off-v-sogo-march-2021-edition-tickets-143909744921 Hosted on Acast. See acast.com/privacy for more information.

Content Note: This episode deals heavily with matters of loss, grieving and addiction. We've made it to our Season Finale! Thanks to everyone who stuck with the first season of IBTFY and helped build this little community. Our final Season 1 guest is LeeAnn Yops, storyteller, comedian and 90210 fan site curator. LeeAnn came to IBTFY to share her love of Luke Perry, right down to "dating" a mini-Luke doll and appearing on the Steve Harvey Show, the 90210 fan community and her experiences dealing with the loss of a favorite cultural icon and a loss much closer to home in rapid succession. Visit LeeAnn's 90210 site here: https://www.bh9021whoa.com/ Check out Appetite here: https://www.facebook.com/AppetiteRnRS/ You can find I'll Be There For You on Apple Podcasts, Stitcher, Spotify or wherever you like to listen to podcasts. We release new episodes every other Sunday to help you beat those Sunday scaries. Please tell your friends, subscribe, give us those sweet reviews! If you have questions, feedback or want to be a guest, you can reach us on most social platforms at @IBTFYPod or illbethereforyoupod@gmail.com. We're booking guests for Season 2, so get at us! Thanks for listening, loves.

GUESTS - Jae Luna & Yip Yops TRACK LIST St. Lucia - Bigger Brasstracks, Thirdstory - Too Far Too Fast Sleeping Lion, Cass Miller - How We Know Yates - Under a Bridge (Premiered on wefoundnewmusic.com) Wafia - I'm Good RuthAnne - Liquid Yoshi Flower - Just Cuz We’re Paranoid Doesn’t Mean They’re Not Onto Us LeyeT - Let Me Know Goldwash - Rhythm Is Frozen The Marías - Cariño Jae Luna - Interview Yip Yops - Interview

YIP YOPS - INTERVIEW by WE FOUND NEW MUSIC

Finally, the crew enters the pirate city of Riddleport! Hey, Grokka the Cruel, here comes trouble!   The awesome background music and ambiance mostly comes from Tabletop Audio.  https://tabletopaudio.com/

On this episode of BSing with Badasses we BS with (and try not to fangirl/boy over) Yip Yops! We chat about what goes into their crazy rad live show, how they met, what they have coming down the pipeline, and more!!

In episode 23 Kristin is joined by fellow 90's kid LeeAnn Yops to discuss Nightmare on Elm Street. LeeAnn is a Chicago comic, storyteller, and creator of Appetite for Rock and Roll- a storytelling show about music and concert experiences that she produces with Kristin and Sara Stock. She is also the creator of bh9021whoa.com - a fan site based on the culture of everyone's favorite zip code (Beverly Hills 90210). The two discussed high school, how Freddy's pedophilia was truly downplayed in the film, and Nancy's mom......oh man Nancy's mom. Follow LeeAnn on Twitter: @LeeAnnYops Appetite is the second Wednesday of every month at the Elbo Room https://www.facebook.com/AppetiteRnRS/ Follow BH9021whoa on Instagram to see all the 90's stars LeeAnn rubs elbows with: @bh9021whoa

Recent studies have identified the tumor suppressor CYLD as a key regulator of NF -κB, a transcription factor that promotes cell survival and oncogenesis, as well as host defence to infection. In th e pr esent study, we investigated the role of tumor suppressor CYLD in regulation of innate immune responses of mice to Y. enterocolitica infection and for comparison to Salmonella Typhimurium infection . Yersinia is an extracellular multiplying bacterium that ensures its extracellular growth by injecting virulence proteins (Yops) into host cells by the injectisome Ysc-T3SS , which interfere with several signaling pathways (such as MAPK and NF-κB cascades), resulting in the inhibition of phagocytosis, oxidative burst and cytokine production. In contrast, Salmonella Typhimurium is endowed with 2 T3SS which inject effector proteins to induce pathogen uptake and intracellular replication. Surprisingly, we found that Cyld-/- mice were more resistant to Y. enterocolitica than Cyld+/- mice in contrast to Salmonella Typimurium infection which appeared to be CYLD- independent. These results suggest that CYLD acts as a detrimental factor for host survival during early Y. enterocolitica infection. Furthermore, we showed that Yops-mediated inhibition of host defense mechanisms , such as phagocytosis, oxidative burst, NF-κB, cytokine production and p38 activation is attenuated in Cyld-/--phagocytic cells in respect of Cyld+/- cells. Taken together, this study provides for the first time, an empirical demonstration of a pathogen-specific contribution of a tumor suppressor gene and its encoded protein, respectively, CYLD, to infection susceptibility in a manner that seems to be independent of its tumor suppression mechanism. This is another example of the extraordinary complexity of the pathogen/host cell interactions.

Die Pathogenität von enteropathogenen Yersinien-Spezies wird von einem Virulenzplasmid pYV kodiert. Das pYV kodiert für den Typ-III-Sekretions-/Translokationsapparat und die Yops. Sechs Yop-Effektorproteine sind bekannt, die in die eukaryote Zelle transloziert werden: YopE, YopH, YopM,YopO/ YpkA, YopP/ YopJ und YopT. Die intrazellulären Angriffspunkte und Wirkungen der einzelnen Yops auf die Zielzelle sind Gegenstand aktueller Forschung. In dieser Arbeit wurden erstmals systematisch potentielle Zytoskelettveränderungen von Thrombozyten durch Inkubation mit Yersinia-Monosekretionsmutanten YopH, YopE, YopO, YopP, YopM und den Yersinia-Monodeletionsmutanten DeltaYopH, DeltaYopE, DeltaYopT, DeltaYopO und DeltaYopM untersucht. Im zeitlichen Verlauf adhärieren uninfizierte Thrombozyten durch eine Zunahme der Fläche durch Ausbildung von Filopodien und Lamellipodien. In den durchgeführten Experimenten ähneln die mit der YopP-Monosekretionsmutante inkubierten Thrombozyten uninfizierten Thrombozyten, d.h. YopP zeigt als Einziges der untersuchtes Yops keinen Einfluss auf das Zytoskelett. Mit der YopH, YopM, YopE bzw. YopO-Monosekretionsmutante inkubierte Thrombozyten zeigen kleinere Zellflächen und eine Dominanz von abgerundeten Zellen. Dies deutet auf eine schlechtere Adhäsion der Zellen an der extrazellulären Matrix hin. In mit WA-C(pTRANS,pCJYM-HA) infizierten HeLa-Zellen konnte YopM zunächst im Zytosol, nach 2-4h Infektion zusätzlich im Zellkern lokalisiert werden. Ausserdem zeigten mit WAC(pTRANS,pCJYM-HA) infizierte HeLa-Zellen Zytoskelettveränderungen: ein ungeordnetes Zytoskelett mit Vakuolen, vereinbar mit einer Ablösung der Zelle von der Matrix. Kaya et al. konnten im Affinitätsblot in vitro eine Interaktion von YopM mit der intrazellulären Cystein-Protease Calpain zeigen. Wegen der morphologischen Ähnlichkeit zwischen mit WA-C(pTRANS,pCJYM) inkubierten bzw. mit Calpeptin, einem Calpain-Inhibitor, inkubierten Thrombozyten wurde die von Kaya et al. dargestellte Interaktion zwischen YopM und Calpain in vivo in dieser Arbeit überprüft. Die Präzipitationsversuche aus infizierten HeLa-Zellen über Calpain sowie über YopM ergaben keinen Hinweis auf eine Interaktion von YopM und Calpain. McDonald et al. publizierten RSK 1 und PRK 2 als intrazelluläre Interaktionspartner von YopM. Funktionelle Studien über die Proteine RSK 1 und PRK 2 lassen darauf schließen, dass sie regulierende Einflüsse auf das Zytoskelett, die Translation und das Zellüberleben haben. Diese Funktionen und die beobachteten Einflüsse von YopM auf das Zytoskelett der Zielzelle sind mit einem Modell vereinbar, in dem YopM die Zytoskelettveränderungen durch Interaktion mit RSK 1 und PRK 2 hervorrufen könnte. Diese Hypothese muss durch zukünftige Forschungen geklärt werden.

Pathogene Yersinien injizieren während einer Infektion über ihr TypIII-Sekretionssystem bakterielle Moduline, sogenannte Yops, in Immunzellen, um das Immunsystem zu stören. Zu Beginn dieser Arbeit war bekannt, dass die injizierten Yersinia enterocolitica-Moduline YopE und YopT das Aktinzytoskelett angreifen. Mit dem Ziel der näheren Charakterisierung der zugrunde liegenden Mechanismen wurde insbesondere der Effekt von Y. enterocolitica-YopE auf aktinregulierende Signaltransduktionswege in menschlichen Endothelzellen (HUVEC) untersucht. Zu diesem Zweck wurde ein Yersinia-Stamm hergestellt, welcher YopE als einzigen Effektor transloziert. Mit diesem Stamm infizierte ruhende HUVEC zeigten eine Veränderung des Aktinzytoskeletts ähnlich wie nach Mikroinjektion von dominant negativem N17Rac, was auf eine Inaktivierung von Rac hindeutete. Zur weiteren Untersuchung wurden in infizierten Endothelzellen durch extrazelluläre Stimuli einzelne Rho-GTPasen aktiviert und die dabei ausgebildeten Aktinstrukturen beobachtet. Dabei ergab sich keine Beeinträchtigung der Neubildung CDC42- und Rho-vermittelter Aktinstrukturen (Filopodien und Stressfasern) durch YopE, jedoch eine spezifische Hemmung Rac-induzierter Lamellipodien. Frühere Untersuchungen hatten demonstriert, dass es sich bei YopE um ein sogenanntes GAP („GTPase activating protein“) handelt, welches in vitro die Proteine Rho, Rac und CDC42 hemmt. Die Ergebnisse der vorliegenden Arbeit weisen darauf hin, dass in Endothelzellen transloziertes YopE höchst selektiv auf die Ras-ähnliche GTPase Rac wirkt, jedoch keinen Effekt auf CDC42 oder Rho ausübt. Diese Ergebnisse zeigen, dass YopE von Yersinia Rho- GTPase-abhängige Signaltransduktionswege mit einer bemerkenswerten Spezifität in primären Zielzellen beeinflussen kann. Überdies wurde die genannte Spezifität auch in primären Makrophagen nachgewiesen. Weiterhin zeigte sich im HUVEC-Infektionsversuch, dass die Hemmung der typischerweise Rho-vermittelten Aktin-Stressfasern YopT-abhängig ist. Morphologische Veränderungen von Aktinstrukturen, wie sie typischerweise bei der Unterbrechung von CDC42- oder Racvermittelten Signalen vorkommen, wurden nicht beobachtet. In Zusammenhang damit konnte gezeigt werden, dass genannter Effekt auf eine chemische Modifikation und folgliche Inaktivierung von RhoA zurückzuführen ist (Zumbihl et al., 1999). Damit unterscheiden sich YopT und YopE in ihrem Wirkmechanismus und spezifischen Zielmolekül, greifen andererseits jedoch beide direkt an Rho-GTPasen an. Sie könnten deshalb synergistisch bei der Pathogenität von Y. enterocolitica wirken. Darüber hinaus könnten YopE und YopT aufgrund ihrer Spezifität zukünftig als wertvolle Hilfsmittel zur Untersuchung zellulärer Regulationsvorgänge dienen

Profilin is an ubiquitous cytoskeletal protein whose function is fundamental to the maintenance of normal cellular physiology. Site-directed mutagenesis of profilin II from Dictyostelium discoideum by PCR resulted in the point mutations W3N and K114E, whereby the W3N profilin is no longer able to bind to poly-(L)-proline concomitant with a slight reduction in actin-binding, whereas the K114E profilin shows profound decrease in its ability to interact with actin but its affinity for poly-(L)-proline remained unaltered. The in vivo properties of the point-mutated profilins were studied by expressing either W3N or K114E in the profilin-minus D. discoideum mutants which have defects in the F-actin content, cytokinesis and development (Haugwitz et al., 1994). Expression resulted in normal cell physiology, a reduction in the F-actin content, and a complete development. Interestingly, only cells which overexpressed W3N could restore the aberrant phenotype, while the K114E profilin with its fully functional poly-(L)-proline binding and its strongly reduced actinbinding activities rescued the phenotype at low concentrations. Both the wild-type and pointmutated profilins are enriched in phagocytic cups during uptake of yeast particles. These data suggest a) that a functional poly-(L)-proline binding activity is more important for suppression of the mutant phenotype than the G-actin binding activity of profilin, and b) that the enrichment of profilin in highly active phagocytic cups might be independent of either poly-(L)-proline or actin-binding activities. To have a better understanding of the in vivo role of profilin, D. discoideum profilin II has been tagged at its C-terminus with the green fluorescent protein (GFP) with a 100-aa linker separating profilin and GFP. This fusion construct was introduced in D. discoideum profilinminus cells and expression of the fusion protein could restore the aberrant phenotype partially. The partial rescue might be due to the uneven expression of the fusion protein leading to mixed populations even after repeated recloning. The profilin-GFP transformants showed normal cell morphology, could be cultivated in shaking suspensions, and could develop fruiting bodies which closely resembled those of the wild-type. In vivo studies revealed the distribution of the fusion protein in highly active regions of the cells such as phagocytic cups, macropinocytotic crowns, cell cortex and at the leading edges of locomoting cells. Thus profilin appears to play a significant role in the regulation of the dynamic actinbased cellular processes. A second actin-regulatory protein from D. discoideum namely, severin, a Ca2+-dependent Factin fragmenting and capping protein, was also investigated via fusion to GFP at its C-terminus. Although severin is a very active F-actin fragmenting protein in in vitro assays, the severin null D. discoideum mutant exhibits normal growth, cell motility, chemotaxis and development. Examination of the live dynamics of severin-GFP should clarify the in vivo role of severin and other functionally redundant cytoskeletal proteins. The 70 kDa severin-GFP fusion protein has been sufficiently expressed and partially purified from the severin null cells whereby in vitro assays confirmed the ability of this fusion protein to sever F-actin only in the presence of Ca2+. Data from confocal microscopy showed that the fusion protein was transiently detected in macropinocytotic crowns, phagocytic cups, membrane ruffles, at the leading edges of motile cells and cell-cell contacts of aggregating cells in directed motion. These data suggest an in vivo role for severin in the remodulation of existing F-actin structures as supported by the in vitro data. The highly dynamic cytoskeleton also plays a significant part in the defence of the cells against pathogens. The behaviour of the actin cytoskeleton of cultured mammalian cells in response to Yersinia enterocolitica infection was examined by confocal microscopy with the aid of GFP-tagged actin, cofilin and profilin II. The translocated Yersinia outer proteins (Yops) encoded by a virulence plasmid in the wild-type bacteria have been observed to disrupt the actin microfilaments, resulting in diffuse actin staining which subsequently disappeared completely upon prolonged bacterial infection. In addition, F-actin structures resembling phagocytic cups were found at the sites of bacterial adherence, suggesting the likelihood of the involvement of the Rho family of small GTPases in the regulation of the actin cytoskeleton. The secreted Yops appeared to have no major effect on the distribution of GFP-profilin whereas the staining pattern of GFP-cofilin seemed to be modified by the Yops, resulting in a decrease in length of the actin-cofilin rods and a diffuse localization of cofilin. The exact mechanisms of interaction between the Yops and their host targets remain to be determined. However, a clearer insight into the interaction between pathogens and the host cytoskeleton will certainly aid in the cellular defence and the prevention of pathogenesis.