Podcasts about Profilin

In this episode of the "Chicken Dinner" podcast, Sam Panayotovich discusses a 14-3 football run, Kevin Durant's sports betting conundrum and Friday's big fight between Jake Paul and Mike Tyson. Special guests Nick Kalikas, Ryan Kramer, Sean Green and Corbie Craig join the show.SUBSCRIBE! Apple, Spotify and all other podcast platformsFOLLOW! X @chickenxdinner | IG and YT @betchickendinner

Episode:  E911 PERSONAL PODCAST – Stylin and Profilin    Description:  Intro: The house is one light.  Subscription: Covid strikes the house, good news about the move and Kelly NEEDS Steve to suffer.    Coupled with Chaos full episodes and bonus content subscriptions are available here:  Premium Content, including Additional 90 Day Fiancé episodes, coverage of other TLC and A&E shows and even some crime news along with more personal podcast episodes are available by subscription at:    Supercast: https://coupledwithchaosnetwork.supercast.tech/  Patreon:  https://www.patreon.com/coupledwithchaos  Apple: Coupled with Chaos Channel: https://podcasts.apple.com/us/channel/coupled-with-chaos/id6442522170    Contacts us:  Email: Coupledwithchaos@gmail.com    Web site: https://coupledwithchaos.com  Facebook: @Coupledwithchaos  Instagram: @Coupledwithchaos    Twitter: @CoupledwChaos   

TICKETS ON SALE NOW For the 2024 Hwy 127 Pickin' The Plateau!!! https://www.eventbrite.com/e/2024-hwy-127-pickin-the-plateau-reseller-event-tickets-811500669347?utm-campaign=social&utm-content=attendeeshare&utm-medium=discovery&utm-term=listing&utm-source=cp&aff=ebdsshcopyurl Follow Caleb On Whatnot https://www.whatnot.com/user/theoldpaths Bama Jaybird Resell YouTube Channel https://www.youtube.com/@UC-dsenCMD_t_6Z8lrq7B6Sw The Old Paths YouTube Channel https://www.youtube.com/channel/UCZSG4_YtxIJPlsk6Nvp84yg If you would like to help support the channel please consider using the links below! Thanks again! *****************************AFFLIATE LINKS**************************** When you click on the # sponsored links to various merchants on this site and make a purchase, this can result in this site earning a commission. # sponsored Link To Caleb's eBay Store https://ebay.us/6SuFMt Buy Caleb A Coffee https://www.buymeacoffee.com/eIsL9EyPvz Need a bank account?? Try Chime and get 100 dollars! https://chime.com/r/tonyanorris17 Find Josh On Instagram https://www.instagram.com/bamajaybirdresell/ Find Caleb On Instagram https://www.instagram.com/caleb_theoldpaths/ Find Caleb On Facebook https://www.facebook.com/caleb.norris.522 ---------------------------------------------------------------- How to listen to our audio podcast! 2 Old Guys Reselling Podcast on Spotify https://open.spotify.com/show/7CZDRWxeITHmh5W0nhDtGc 2 Old Guys Reselling Podcast on Amazon Music https://music.amazon.com/podcasts/5a155caf-e48a-4d73-9d74-eb302d12fc7c/2-old-guys-reselling-podcast 2 Old Guys Reselling Podcast on Apple Podcasts Connect https://podcasts.apple.com/us/podcast/2-old-guys-reselling-podcast/id1683575891 2 Old Guys Reselling Podcast on iHeartRadio https://iheart.com/podcast/113494540/ 2 Old Guys Reselling Podcast on Pocket Casts https://pca.st/zmhciakt 2 Old Guys Reselling Podcast on Radio Public https://radiopublic.com/2-old-guys-reselling-podcast-WdrB5r 2 Old Guys Reselling Podcast On Pandora https://www.pandora.com/podcast/2-old-guys-reselling-podcast/PC:1001070101 2 Old Guys Reselling Podcast On Overcast https://overcast.fm/itunes1683575891 2 Old Guys Reselling Podcast On Audible https://www.audible.com/pd/2-Old-Guys-Reselling-Podcast-Podcast/B0BZNQ3YVT?action_code=ASSGB149080119000H&share_location=pdp ----------------------------------------------------------------- If you would like to send us stuff send us an email!! 2oldguysreselling@gmail.com #podcast #sidehustle #resellingpodcast podcast eBay selling eBay reselling eBay sales eBay buying 2 Old Guys Sourcing Reselling Shed Storage --- Send in a voice message: https://podcasters.spotify.com/pod/show/2-old-guys/message

Google Search now supporting Discussion Forum and Profile Page Data Google Search now supporting Discussion Forum and Profile Page Data This week on Marketing O'Clock, get rich or data tryin', profiles, discussions, forums and organization structured data is here. Plus, a new addition to video campaigns is coming to Google Ads - and we'll give you the long and the SHORT of it. Also, Chrome is where the cart is - Merchant Center promotions can now show in the Chrome browser. Visit us at - https://marketingoclock.com/ Join our Discord Community - http://community.marketingoclock.com/ Join Marketing O'Pick'em (NFL picks) - https://www.runyourpool.com/p/j/fb9b6d706ebf46cc931ea45ab3668e47 -------------------------------------------------------------------------------------------------------------------------------------------- Intro - 00:00

#RingRust with my #VictoryRoad chat... & I pay musicular tribute to the 1st NWA World's Heavyweight title win of everybody's favourite apparently-immortal WWE Hall of Famer, in this week's #3WayDanceOff!  ~ ~ ~ I'd like to hear from you! Please drop me a line @ ring-rust@hotmail.com {Subject Line: Ring Rust} & let me know what you like {or dislike} about my show! I'm always on the lookout for constructive criticism {if you want playlists again, start giving me feedback, people!}  ~ ~ ~ Check out my #Unboxing videos, all that snazzy anti-social media & support all my shows http://markjabroni.mysite.com/ ~ ~ ~ RECORDED LIVE @ CHMR-FM Studios in Sunny St. John's NL! Learn more @ https://www.chmr.ca/ If you want to contribute to Betty Cisneros' Stage 4 Cancer treatment, please donate @ https://www.gofundme.com/f/help-betty-battle-her-cancer-away SHOW NOTES... 0:05:00 Pay-Per-Review: Impact Wrestling's Victory Road 1 0:07:03 Musicular Interlude 1 0:17:08 Pay-Per-Review: Impact Wrestling's Victory Road 2 0:19:36 Musicular Interlude 2 0:31:19 Pay-Per-Review: Impact Wrestling's Victory Road 3 0:33:19 Musicular Interlude 3 0:40:18 Pay-Per-Review: Impact Wrestling's Victory Road 4 0:42:33 Musicular Interlude 4 0:50:39 Assuming the Intermissionary Position 0:55:41 This Week's Macho Fact 1:04:43 Pay-Per-Review: Impact Wrestling's Victory Road 5 1:07:14 Musicular Interlude 5 1:16:39 Pay-Per-Review: Impact Wrestling's Victory Road 6 1:19:25 Musicular Interlude 6 1:28:17 This Week's 3-Way Dance-Off: Stylin' & Profilin' to the World's Heavyweight title!  1:39:55 Pay-Per-Review: Impact Wrestling's Victory Road 7 1:41:49 Musicular Interlude 7 1:48:47 Podcast Extra: Bet You Forgot These Wrestlers Were Superstars 2 Decades Ago 1:56:20 Musicular Interlude 8

Xhenis Rama është ndër të tjera, Personal Trainer, Asistente adminstrative, Infermiere dhe Kërcimtare. Nga ekperianca e saj në trajnim fizik të klientëve të ndryshëm, Xhenis ndan në podcast këshilla dhe vrejtje rreth kujdesit fizik vetjak por jo vetëm. Për më shumë vizitoni Profilin e saj në Instagram: rama_xhenis ( https://www.instagram.com/rama_xhenis/ ) ku mund ta kontaktoni për pyetje dhe interesa specifik. Qëndroni të shëndetshëm! Ky episod i Buchi Podcast do të fokusohet në temën e kujdesit për trupin dhe fitness. Dëgjuesit do të mësojnë rreth rëndësisë së kujdesit për trupin dhe mënyrave se si të arrijnë dhe të mbajnë një formë fizike të mirë. Faleminderit që na dëgjoni! Ju Lutemi për support duke e shpërndarë podcastin mes miqve tuaj, duke komentuar mendimet tuaja dhe normalisht bëni LIKE komentoni dhe shpërndajeni me miqtë tuaj. Mund gjithashtu të na supportoni ne Patreon ose BuyMeaCoffee! Na ndiqni ne Instagram ,Facebook dhe Tweeter! Degjim të kendshëm dhe Respekte! i-Optika Adressa Thoma Koxhaj, prane pallateve Cabej, Yzberisht, Tirane +10% ulje me kodin BUCHIPODCAST i-Optika IG : https://www.instagram.com/i_optika_/ Mund të na gjeni ne: Patreon: https://www.patreon.com/buchipodcast Facebook: https://www.facebook.com/buchi.podcast Instagram: https://www.instagram.com/buchi_podcast/ Buchi Podcast ofron një platformë unike për njerëzit që dëshirojnë të njohin më shumë rreth botës së podcasteve në gjuhën shqipe. Ky podcast ofron një gamë të gjerë të temave dhe pikëpamjeve që i bëjnë tërheqës për një audiencë të gjerë. Nga diskutimet mbi kulturën e teknologjisë, në diskutimet rreth politikës dhe shoqërisë, Buchi Podcast ofron diçka për secilin dëgjues. Përveç kësaj, Buchi Podcast është i realizuar me një cilësi të lartë të produksionit dhe përdor një stil të qartë dhe të kuptueshëm të prezantimit. Ky podcast siguron që dëgjuesit të ndihen të përfshirë në diskutimet dhe të ndjehen si pjesë e një komuniteti që ndajnë të njëjtat interesa. Buchi Podcast gjithashtu ofron një mënyre të re dhe unike për të mësuar në një mënyrë të argëtueshme dhe të këndshme. Në këtë podcast, nuk ka asnjë kufizim për temat që diskutohen, dhe kjo i jep dëgjuesve një mundësi të jashtëzakonshme për të mësuar diçka të re dhe të ndërlikuar në një mënyrë të thjeshtë dhe të kuptueshme. Në përgjithësi, Buchi Podcast është një podcast i vlerësuar për një audiencë të gjerë që dëshiron të njohë më shumë rreth botës nëpërmjet një platforme të qartë dhe të këndshme. Unë besoj se njerëzit do të gjejnë këtë podcast të dobishëm dhe tërheqës, dhe se do ta ndjekin me kënaqësi. Podcastin dhe Monologjet i gjeni ne çdo lloj platforme që ofron këtë shërbim. #buchipodcast #fitness #personaltrainer Nëse jeni një person që kërkon një podcast në shqip që trajton tema të ndryshme në një mënyre interesante dhe informuese, atëherë Buchi Podcast është pikërisht ajo që ju duhet.

Many people may have been told about learning styles. Which usually means they've been told they are either a visual learner, an auditory learner, or a hands-on learner. But what if that isn't quite right? What if we're not thinking about learning styles in the best way possible? Listen in as we discuss what learning styles are, what variables help to drive learning, and how may be able to use the learning styles in a more productive way.

Tim and AZ shot the shit about everything and nothing all at the same time! --- Send in a voice message: https://anchor.fm/popculturepros/message Support this podcast: https://anchor.fm/popculturepros/support

We talk all things wrestling, personal life, and about StarCast happening this July! Buy a ticket or watch on television as Ric Flair appears in his last match! The Roast of Ric Flair is Friday, July 29, 2022 and StarCast is Sunday, July 31!! --- Send in a voice message: https://anchor.fm/excusemevickie/message Support this podcast: https://anchor.fm/excusemevickie/support

Sean Magnum, Danny Vagrant, & Donnie Vagrant interview Danny Vapid, John Wayne Gacy impersonator at Thunder Over Louisville, Alfred Hitchcock, Ramones & Regis, & even more. --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app --- Send in a voice message: https://anchor.fm/sean0493/message Support this podcast: https://anchor.fm/sean0493/support

Well in honor of Cinco De Mayo coming up I decided it was time to do another Stylin' & Profilin' episode. I bring to the table three local craft Mexican Lagers for us to try. Joining me again is Chasta but for this episode we are joined by Dennis Willis of Soundwaves TV and Baby Huey.  See omnystudio.com/listener for privacy information.

It's time to get educational! That's right, as much as I love the fact that you listeners are learning about new and exciting breweries, I want to help teach you about the beers themselves as well. That's why, with the help of Chasta we are starting a new sub series in BONE-A-Fide Brews, Stylin' and Profilin'. We will take a look at the history and backstory of one specific style and because Chasta is the IPA queen we dive into the West Coast IPA, and drink some as well! See omnystudio.com/listener for privacy information.

Back into Human Design we go! This time, it's all about profiles, the unique archetypes and characters that each of us play. Jenn and Mik also talk about their new favorite reality show.Some things that we talked about in this episode:We just started watching Love Off the Grid (on Discovery+). It's an interesting ride of “homesteaders” trying to get their urban boos to love living off the grid as much as they do.Interested in the I Ching? You can pick up a copy on Amazon (this is the copy Jenn has). Jenn also wants to pick this one up too by Chetan Parkyn (THE BOOK OF LINES)*And this Human Design book by Chetan Parkyn (has more juicy content on profiles (including compatibility).* Join our Patreon community for more exclusive content.*Note: As Amazon Associates, we earn from qualifying Amazon purchases.Support the show (https://www.patreon.com/chakrasandshotguns)

"Roy Dale Cox, the Bama Boy from Birmingham, Alabama was born to entertain." Roy Dale Cox takes us on a journey on being a fashion creator and Icon. roydalecox.com Instagram.com/roydalecox --- Send in a voice message: https://anchor.fm/thefrontporchpod/message

Meet the man who started the Chosen Lawyer on the road to being the most stylish lawyer! Ed Arabian is the owner of Ed's Fine Imports - a leading provider of men's dress clothing and accessories. Ed has been styling the Chosen Lawyer for over a decade and he brought his fashion tips to the Chosen Life! On today's episode, Ed discusses the HOTTEST Men's fashion trends for 2022. He also shared the biggest fashion mistakes that men make and what they should wear on a date. If you are ready to style and profile - you don't want to miss this one! Please make sure to subscribe to our channel, hit the notification bell to learn of future episodes of The Chosen Life, and most of all, leave your comments below! We would love to hear from you and your thoughts on today's topics. The Chosen Life is all about community and we value your views! 0:00 - Intro: Meet Ed Arabian 20:54 - Hottest Men's Fashion Trends in 2022 30:06 - Biggest Fashion Mistakes Men Make 40:28 - What to Wear on a Date 55:33 - Outro ____________________________________________________________ STAY CONNECTED:

In this throwback episode of The Power Producers Podcast, David Carothers and co-host Kyle Houck interview Walt Goshert, Insurance LinkedIn Expert and Independent Marketing Consultant. Walt discusses the value of understanding workers' compensation and the benefits of using LinkedIn as a platform for building strategic relationships.   Episode Highlights: David introduces Walt Goshert. (1:25) Walt shares his background in the insurance business. (2:31) Walt says that he finds the insurance somewhat easy to understand, but at the same time boring as well. (4:45) Walt likes to create a process of making a person trust you to be their advisor. (4:57) Walt says that insurance has developed into a higher marketing type of discipline. (5:22) What are we doing in our interactions and relationships with people to allow us to be perceived as respected, trusted advisors? (9:50) Walt says that the producers should have the initiative to step up, initiate the relationship, and create the perception of value. (10:35) David shares that a lot of agents are not willing to go the extra mile to invest in learning and understanding something that will make them successful in the future. (12:27) David mentions that they view insurance as a commoditized shuffling of paperwork. (23:10) David says the Bureau of Labor Statistics shows that indirect costs of any claim across the general industry are anywhere between two and 20 times the direct cost. (23:49) Walt says that one of the criteria of being a great producer is you need to be curious and fascinated about businesses. (32:47) Walt says that video communication is a neat way to do business and also time-efficient for you and the client. (48:21) David shares that we need to change the way we think to deliver the message to people the way they wish to receive it, not how we perceive they need to hear it from us. (49:50) What are the three tips that Walt would give to a LinkedIn newbie? (55:15) What are the three things anybody can do to set themselves on the right course (55:23) Tweetable Quotes: “You can't force someone to make a change, they have to internalize making that change for their own benefit.” - Walt Goshert “We do business with people, we don't do business with businesses.” - Walt Goshert “You really need to plant your flag in a target market.” - Walt Goshert “Everyone needs to have a prospecting habit and a prospecting process that you religiously follow each and every day.” - Walt Goshert Resources Mentioned : David Carothers LinkedIn Kyle Huock LinkedIn Florida Risk Partners Walt Goshert LinkedIn floridariskpartners.com The Extra 2 Minutes                        

Steve's impeccable style is still on display in Dubai. He will also be going to a very special location for the upcoming Miss Universe pageant. Learn more about your ad-choices at https://www.iheartpodcastnetwork.com

We're back dudes! Talking Oktoberfest. Beers tight. You're tight.

Frank and Heather answer a listener question and discuss weddings, Footloose, the Taliban, and danger on set. --- Send in a voice message: https://anchor.fm/heather-whaley6/message

Germantown native, AyeshiA Smith, is both CEO and Fashion Designer of the next big global brand, AyeshiA, an all-inclusive luxury women's wear brand designed for women who appreciate timeless custom made pieces that creates confidence. AyeshiA's passion for fashion started at the age of five when she would often frequent Hancock Fabrics with her mom. At the age of thriteen she started sewing and repurposing denim jeans into designer handbags and selling them at her high school. Since then, she launched her business in January 2013 and the rest is history. She has showcased her line in several fashion shows across the country including LA Fashion Week and next up she will be showcasing her newest collection, “Memphis” at New York Fashion Week this September 11th and 12th. Balancing being the boss of her brand and a fulltime mom to her two kids is challenging, but so rewarding. AyeshiA has upcoming showcases in both New York and California in 2022. Be on the lookout for more to come with vibrant and timeless brand, AyeshiA.

Germantown native, AyeshiA Smith, is both CEO and Fashion Designer of the next big global brand, AyeshiA, an all-inclusive luxury women's wear brand designed for women who appreciate timeless custom made pieces that creates confidence. AyeshiA's passion for fashion started at the age of five when she would often frequent Hancock Fabrics with her mom. At the age of thriteen she started sewing and repurposing denim jeans into designer handbags and selling them at her high school. Since then, she launched her business in January 2013 and the rest is history. She has showcased her line in several fashion shows across the country including LA Fashion Week and next up she will be showcasing her newest collection, “Memphis” at New York Fashion Week this September 11th and 12th. Balancing being the boss of her brand and a fulltime mom to her two kids is challenging, but so rewarding. AyeshiA has upcoming showcases in both New York and California in 2022. Be on the lookout for more to come with vibrant and timeless brand, AyeshiA.

You need a stylist? Need a one stop shop? DevinAire is your place to go! Tune in and Check him out --- This episode is sponsored by · Anchor: The easiest way to make a podcast. https://anchor.fm/app Support this podcast: https://anchor.fm/aujahnae-coady/support

This is the interview with Joey C. and Antonio Brazil calling in from Dallas Texas. 

Special guest AJ Jones tells us what its like to be a male hair stylist in Appalachia. Numerous topics are talked about to include conspiracy theories, politics, becoming a Bohemian minimalist and being black in Appalachia. --- Support this podcast: https://anchor.fm/grunts-in-appalachia/support

Welcome back to the podcast, the CourtSideHeat Overtime edition podcast, as we break down EVERYTHING in the world of news. Today, we are breaking down some of these topics: the Cuomo's killers? BLM doesn't give a darn about Breonna Taylor's mom? Does China want MORE slaves? And finally, unity for all can happen and is happening. The media, and THEM, want us to be divided but for the majority of Americans we stand united... without the news, elitists, politicians, and/or any other agenda! This is a new podcast channel that is between 30-50 minutes long starting every other Wednesday at seven in the morning. Check this and more to start your day off right. You can find us on Spotify, SoundCloud, iTunes, Rumble, YouTube, DailyMotion, Google Podcasts, our website (CourtSideHeat.com), and even more and more places as we can find them! Also, head over to Stylin' Profilin' Gear shop as we are introducing the most amazing, high volume, and high in-demand swag you'll ever see! Click the link here to go to our Spreadshirt shop: https://shop.spreadshirt.com/stylin-p...​

Wooooooo! Giving a breakdown of the American brown ale. Make beer. Drink beer. Be tight.

Giving you the runaround on the old APA. Make beer. Drink beer. Be tight.

Join Rachael and Jen as they discuss how beer gets categorized into styles and share a few tips for entering beers into competitions.

Real Guys, Real Life, Real Situations...Real Funny?! The show about nothing & everything regarding social skillz returns. HoHs break their hiatus w/ a new HoH. The guys intro a new embarrassing segment & discuss shameful past choices in attire. They explore the efforts of humanity to stay up on the freshest duds versus those trail blazers who double down on one classic style profile. Some wisdom is sprinkled in on how to Costco better, like James Bond.

This week, Fleeger and Briggs discuss the World Series and the impact of analytics over common sense and feel. They also break down changes to the Maaters, decalre the winner in the Brady vs Belichick debate, and give their mid-season NFL power rankings. The guys wrap up the show by picking every Week 8 NFL game straight up and against the spread.

Welcome to Episode 16 of the But I Could Be Wrong Podcast. We're here to have fun, entertain, laugh, and inform. Subscribe for more exclusive But I Could Be Wrong content:http://bit.ly/YTBICBWNew videos every Thursday! Find us online:https://linktr.ee/buticouldbewrongAbe: @abe__cityJohn: @johntherapperPeter: @hundleyjunior#podcast #podcasts #buticouldbewrong

This week, Fleeger and Briggs discuss the World Series and the impact of analytics over common sense and feel. They also break down changes to the Masters, declare the winner in the Brady vs Belichick debate, and give their mid-season NFL power rankings. The guys wrap up the show by picking every Week 8 NFL game straight up and against the spread.

Join us as we welcome special guest Christie Grays Chambers, expert fashion stylist and consultant from I AM CHIC (www.iamchic.net) and consistently voted as Miami's best dressed business woman, as she provides her insights on the hot fashion trends for both men and women as we continue through the summer and enter the fall seasons.  Christie also shares her insights on great places to find your next wardrobe or two online or in person.  Learn more about Christie at www.iamchic.net and on Instagram at @i_am_chic_10.  

In this episode of The Power Producers Podcast, David Carothers and co-host Kyle Houck interview Walt Goshert, Insurance LinkedIn Expert and Independent Marketing Consultant. Walt discusses the value of understanding workers’ compensation and the benefits of using LinkedIn as a platform for building strategic relationships.   Episode Highlights: David introduces Walt Goshert. (1:25) Walt shares his background in the insurance business. (2:31) Walt says that he finds the insurance somewhat easy to understand, but at the same time boring as well. (4:45) Walt likes to create a process of making a person trust you to be their advisor. (4:57) Walt says that insurance has developed into a higher marketing type of discipline. (5:22) What are we doing in our interactions and relationships with people to allow us to be perceived as respected, trusted advisors? (9:50) Walt says that the producers should have the initiative to step up, initiate the relationship, and create the perception of value. (10:35) David shares that a lot of agents are not willing to go the extra mile to invest in learning and understanding something that will make them successful in the future. (12:27) David mentions that they view insurance as a commoditized shuffling of paperwork. (23:10) David says the Bureau of Labor Statistics shows that indirect costs of any claim across the general industry are anywhere between two and 20 times the direct cost. (23:49) Walt says that one of the criteria of being a great producer is you need to be curious and fascinated about businesses. (32:47) Walt says that video communication is a neat way to do business and also time-efficient for you and the client. (48:21) David shares that we need to change the way we think to deliver the message to people the way they wish to receive it, not how we perceive they need to hear it from us. (49:50) What are the three tips that Walt would give to a LinkedIn newbie? (55:15) What are the three things anybody can do to set themselves on the right course (55:23) Tweetable Quotes: “You can't force someone to make a change, they have to internalize making that change for their own benefit.” - Walt Goshert “We do business with people, we don't do business with businesses.” - Walt Goshert “You really need to plant your flag in a target market.” - Walt Goshert “Everyone needs to have a prospecting habit and a prospecting process that you religiously follow each and every day.” - Walt Goshert Resources Mentioned : David Carothers LinkedIn Kyle Huock LinkedIn Florida Risk Partners Walt Goshert LinkedIn floridariskpartners.com The Extra 2 Minutes                                                                                                     

Christopher Michaels and Matt Wylde explore the career and title reigns of the Man the Nature Boy Ric Flair. Download the New Single from Christopher Stolle Here: https://realmofthemistente.wixsite.com/realmofthemistent/copy-of-christopher-stolle-1 Publicist: SJ Promotions Contact: Steven Joiner Cell: (816) 605-4561 Email: Stevesjnetwork@gmail.com ------------------------------------------------------------------------------------------------------------------- Contact us: Email: realmofthemistentertainment@gmail.com Phone: (267) 490-3166 YouTube Edits: Chris Stolle using Hitfilm Software All content copyrighted under Realm of the Mist Entertainment, All Rights Reserved. Help is there: National Suicide Pervention Hotline: 1-800-273-8255 ------------------------------------------------------------------------------------------------------------------- Please Support Us: https://anchor.fm/rotm-entertain/support Support us here: https://www.subscribestar.com/realm-of-the-mist-entertainment And on Patreon: https://www.patreon.com/Realmofthemist Music: https://www.bensound.com ------------------------------------------------------------------------------------------------------------------- Now on Pandora: http://pandora.app.link/Z4uEsRezm3 Anchor.fm: https://anchor.fm/rotm-entertain Google Podcast: https://www.google.com/podcasts?feed=aHR0cHM6Ly9hbmNob3IuZm0vcy85Njg3NjY4L3BvZGNhc3QvcnNz Spotify: https://open.spotify.com/show/1tcvwQIHT0xD60glRXdfUa Breaker: https://www.breaker.audio/realm-of-the-mist-entertainment Pocketcast: https://pca.st/6WaV Radiopublic: https://radiopublic.com/realm-of-the-mist-entertainment-WDJbVl Realm of the Mist Youtube: https://www.youtube.com/realmofthemistentvape Sounds Dicey Gaming YouTube Channel: https://www.youtube.com/channel/UC0yrlmq0iU9pmo46yBpA73A Realm of the Mist Webite: https://realmofthemistente.wixsite.com/realmofthemistent Follow Realm of the mist on Twitter: @RealmMistent https://www.patreon.com/rss/Realmofthemist?auth=1ae4d4aec78817c4611690f484ed5ef5 --- Support this podcast: https://anchor.fm/rotm-ent/support

The two-time WWE Hall of Famer and 16-time World Champion looks back at his incredible retirement weekend ahead of the premiere of WWE 24 Ric Flair: The Final Farewell. Plus, Darren Young & Titus O’Neil discuss the current situation in the United States, JTG remembers his late friend and tag team partner Shad Gaspard, and Drake Maverick opens up about his Cinderella run in the Interim NXT Cruiserweight Championship Tournament. See omnystudio.com/listener for privacy information.

This week the guys are prompted to talk a bit about art style. They mention some creators who inspire them, drink a couple beers, touch on the end of the world and much more! // Become a Patreon and get early video versions of each episode // Visit www.tigshow.com/merch to pick up one of our new shirts, coasters or stickers! // Our intro is by Gary Fitzgerald with music by Grey Watson. You can follow the show on twitter, Untappd and instagram at @tig_show. // #comics #podcasts #beer #freelance #coronavirus #covid19 #artstyle #art --- Send in a voice message: https://anchor.fm/tigshow/message Support this podcast: https://anchor.fm/tigshow/support

Wooooo! Yup, this is the first time Ric Flair ventured into ROH territory and man it actually felt like he wanted to be there that first day! Promos in advance of the appearance, an autograph signing beforehand, and even some in-ring activity to cap off the show! Plus, a legendary video segment with Larry Sweeney that was recorded for a future Video Wire. Plus, the show is one heck of a sleeper, easily forgotten to the memory vault, but chock full of great matches, even if we get some weak finishes to go along with them. A six man tag that steals the show, a tag match full of drama and intensity, a hellacious opener, and more! It's “Stylin & Profilin” on this week's episode of An Honorable Mention, and as always we are hosted by the Creative Control Network, plus sponsored this week by Podcorn.com!0-0-0-0-0-0-0-0-0-0-0-Stylin & ProfilinCollinsville, IL3/13/09Dark Tag Team MatchShawn Schultz & Sigmon vs. Aaron Scott & Grizzly RedwoodDark MatchAlex Payne vs. Travis SawyerSingles MatchRoderick Strong vs. Jay BriscoeSHIMMER Title MatchMsChif (w/Delirious & Jimmy Jacobs) (c) vs. Daizee HazeFour Corner Survival MatchClaudio Castagnoli vs. Austin Aries vs. Silas Young vs. Kenny OmegaSix Man Tag Team MatchBobby Dempsey, El Generico & Kevin Steen vs. Sweet 'n' Sour Inc. (Chris Hero, Davey Richards & Eddie Edwards) (w/Larry Sweeney & Shane Hagadorn)Tag Team MatchKenny King & Rhett Titus vs. Irish Airborne (Dave Crist & Jake Crist)Singles MatchBryan Danielson vs. Bison SmithTag Team MatchJerry Lynn & Tyler Black vs. The Age Of The Fall (Delirious & Jimmy Jacobs)ROH World Title MatchNigel McGuinness (c) vs. Brent Albright -=-=-=-=-=-=-An Honorable Mention Podcast is hosted by Jeff Schwartz & Shane Hagadorn every Tuesday on the Creative Control Network. OUTLETS:iTUNES: https://itunes.apple.com/us/podcast/an-honorable-mention-with-shane-hagadorn-jeff-schwartz/id1348324250?mt=2TuneIn Radio: https://tunein.com/podcasts/Sports--Recreation-Podcasts/An-Honorable-Mention-p1101966/ Stitcher: https://www.stitcher.com/podcast/an-honorable-mention?refid=stprSpreaker: https://www.spreaker.com/show/an-honorable-mention-with-shane-hagadorn Castbox: https://www.castbox.fm/channel/id2083202 Podbean: https://www.podbean.com/podcast-detail/6xh9y-77731/An-Honorable-Mention-with-Shane-Hagadorn--Jeff-Schwartz-PodcastSpotify: https://open.spotify.com/show/3bT7qZVq6PN2JWJu21v0jMiHeartRadio: https://www.iheart.com/podcast/966-an-honorable-mention-w-sha-45911710/ListenNotes: https://www.listennotes.com/podcasts/an-honorable-mention-with-shane-hagadorn-0nVes3d7roi/Podcast Addict: http://podplayer.net/?podld=2360081Podchaser: https://www.podchaser.com/podcasts/an-honorable-mention-w-shane-h-625260SUPPORT THE SHOW:Patreon.com/AnHonorablePodADVERTISE WITH US:advertisecast.com/anhonorablementionwithshanehagadornjeffanhonorablemention@gmail.comFOLLOW US FOR THE LATEST NEWS & NOTES!Twitter & Instagram- @anhonorablepodTwitter & Instagram - @thejeffschwartzTwitter & Instagram - @hagadornshaneAudio by Zach Johnson - @RadioZTanhonorablemention.wixsite.com/mainTwitch.tv/AnHonorablePodFacebook.com/AnHonorablePodYoutube Search “An Honorable Mention”

Bob, Mike and Zaq discuss listener questions gathered from social media surrounding Bob's interview with Danny Hartley's cousin, Michelle.Today's Sponsors: wearfigs.com - Enter code "Truth" at checkout and get 15% off your first purchase. upstart.com/truth - Find out how low your Upstart rate is.

The Fire Tom Herman Podcast turns over a new leaf as Josh and Noah discuss a big week in the recruiting world of Oscar Giles and Big 12 Media Days.

Back from a two-week holiday break, the SwissPats welcome on Sophie Adang, of Stylemixers. Sophie is a fashion consultant based in Basel who shows people how to choose clothing that suits them. Suzi and Don also discuss how Don deeply scarred his animal-loving son while visiting Iceland, how many Swiss cities are ranked in Mercer’s Quality of Living Reports, which offers city-to-city comparisons for nearly 500 global assignment destinations, how A Tribe Called Quest assisted an Emmental cheese maker and what are the chances a third class is added to rail travel in Switzerland.

On this 14th day of the Government Shutdown, Pickles and Pizza (and D3L) are Podcasting from Sunny South Florida with a brand new episode, featuring Eddy Shipek aka “MR. SUNSHINE” — an Entrepreneur (Locksmith/Realtor), Live Video Social Media Guru, and Palm Beacher with a plethora of knowledge about Local History, an eye for fashion, and always emanating a positive aura. The following conversation covers various subject matters, including, but not limited to: Stylin’ and Profilin’, Motorcycle Outfits, Crazy Locksmith Stories, Mar-a-Lago, Palm Beach Life & Fashion, the Constitutional Necessities of the Electoral College, Patreon, ChangeTheTerms.org (who defines “Hate Speech”?), Kanye’s Tweets and “Pocahontas” Elizabeth Warren Announcing Her Potential Run for POTUS. This episode was produced by D3L at West Palm Beatz.  #MotorcycleOutfits #SunnySouthFlorida #PalmBeachStyle #PalmBeachFashion #KanyeWest #POYB #Podcast #BobbyPickles #TheRealMisterPickles #NotKidding #4GxG #MADEinaSWEATSHOPintheUSA

Hey guys, Landry Miller and Quinn Blakely here to hijack all the Channel Four and a Half Podcast Feeds to share with you all of the best moments and podcast episodes from our network with you! In this special show - you’ll hear all the best episodes of the year from, Opinions Like A-Holes, Digital Intercourse, Talk Show Incorporated, the barely legal Everybody Loves Amon, Happy Horror Coffee Break, Stylin and Profilin w/ Cam & Zam, and the People Person’s Paper Podcast! We have so many huge things coming in 2019 including a whole slew of new shows, content, and ways for you to listen - so stick with us - and we’ll save you from all harm!Happy New Year, friends! Check out our new sponsor PeggingToys.com they are the best in prostate play- check out their huge list of products both for prostate play, pleasure, and hygiene! Use our promo code “digital” at checkout to save 10% and support this show! Hosted by Tom King, Lauren TurnerAudio Engineering by Landry MillerExecutive Produced by Michael Zampino, Hilton Price, and Landry MillerPowered by Channel Four and a Half

Hey guys, Landry Miller and Quinn Blakely here to hijack all the Channel Four and a Half Podcast Feeds to share with you all of the best moments and podcast episodes from our network with you! In this special show - you’ll hear all the best episodes of the year from, Opinions Like A-Holes, Digital Intercourse, Talk Show Incorporated, the barely legal Everybody Loves Amon, Happy Horror Coffee Break, Stylin and Profilin w/ Cam & Zam, and the People Person’s Paper Podcast! We have so many huge things coming in 2019 including a whole slew of new shows, content, and ways for you to listen - so stick with us - and we’ll save you from all harm!Happy New Year, friends! This week's sponsor: Audible.com www.audibletrial.com/opinions

Hey guys, Landry Miller and Quinn Blakely here to hijack all the Channel Four and a Half Podcast Feeds to share with you all of the best moments and podcast episodes from our network with you! In this special show - you’ll hear all the best episodes of the year from, Opinions Like A-Holes, Digital Intercourse, Talk Show Incorporated, the barely legal Everybody Loves Amon, Happy Horror Coffee Break, Stylin and Profilin w/ Cam & Zam, and the People Person’s Paper Podcast! We have so many huge things coming in 2019 including a whole slew of new shows, content, and ways for you to listen - so stick with us - and we’ll save you from all harm!Happy New Year, friends!

Hey guys, Landry Miller and Quinn Blakely here to hijack all the Channel Four and a Half Podcast Feeds to share with you all of the best moments and podcast episodes from our network with you! In this special show - you’ll hear all the best episodes of the year from, Opinions Like A-Holes, Digital Intercourse, Talk Show Incorporated, the barely legal Everybody Loves Amon, Happy Horror Coffee Break, Stylin and Profilin w/ Cam & Zam, and the People Person’s Paper Podcast! We have so many huge things coming in 2019 including a whole slew of new shows, content, and ways for you to listen - so stick with us - and we’ll save you from all harm!Happy New Year, friends! Listen on Stitcher Listen on Itunes Watch a previous episode, here:

On this episode, Fred and Chris are joined by Devan Corbello of Corbello's Menswear to fashion trends, business, and candy. Fred Sebren is on a mission to inspire veterans and active duty military to reach their true potential.  He has dedicated his life to empowering those who have served by helping them restore their credit, get their personal finances in order, and build a life of their dreams through business ownership.  The recipe is simple.  Gain knowledge and take action!   

Patrick Bet-David and "The Nature Boy" Ric Flair take a trip down memory lane discussing Flair's days in high school, his close calls in life with drinking and girls, his health recovery and next entrepreneurial endeavors with The Ric Flair Collection | Stylin’ and Profilin’ with Mr. Custom Made.

On the heels of a big announcement about All In and Starrcast, your buddies return to talk ROH, relationships, Money in the Bank and more! Spend an hour with us and enjoy an awesome Wrestling Confession Wreturn and a classic FMK. The Nature Boy makes a visit, along with a press conference villain, a little boy, and one of the few mother's Kevin Owens sent well-wishes to this past Sunday. Strap in and Let's Podcast!

The PSBF co-hosts take a more unorthodox approach to this week's Cocktail Hour, as Anthony yells about the Penguins beating the Flyers, Jeffrey gives a behind the scenes look at how he wrote his God of War review, and Miguel recaps WrestleMania. Grab a drink and come hang out! Don't forget to check us out on Patreon, $2 gets you a shoutout on the show and $5 let's you choose your own topic for us to discuss! http://www.patreon.com/psbestfriends --- Send in a voice message: https://anchor.fm/psbestfriends/message

Ryan, Marques and Ricky discuss random topics such as gingerbread houses.  There was no agenda on this day because they were gone for so long.  Huge apologies.   Follow Ryan on twitter @gullyblanchard and Ricky @RdotCongoTweets.  We are also on Instagram @gullyblanchardpodcast.

This week the squad reunites and talks the WILD week of Rasslin'...we talk the up coming Jericho/Omega match at WrestleKingdom, the title changes at the UK TV tapings in WWE, the Ric Flair 30 for 30 doc and fo course the week that was in Black Rasslin'.

Damn, we had fun this week! Rog Smuve got to attend the Jay-Z 4:44 tour so we talked about it, the poor ticket sales, and how Jay-Z ranks as a live performer (03:00). We checked in with Tyrese (21:00) before discussing Meek Mill (24:00) and Liangelo Ball (30:00) and their latest legal troubles. After the awesome ESPN 30 for 30 documentary on Ric Flair, we took some time to talk about the Nature Boy and his 10,000 confirmed kills (36:00) before listing our Commandments for Social Media Etiquette (54:00). We wrapped the podcast with the weekly #MeToo report (1:10:00), Aldo’s Nothin’ NICE to Say about the home team (1:12:00), and broke some new music from our favorite Panamanian, Nesto DP (1:17:00).

The week in review jam packed in this short time. Starting with The Man.. The Nature Boy Ric Flair's 30 for 30. Koola tries to speed through Impact's Bound For Glory but Alex wants to be informed about it so he slows it down. We dive into the Shields necessity of being reunited again x 10. We're happy about finally seeing other cruiserweights and the UK tournament's contenders again. Sami & K.O. unhappy??? The viciousness of the UFC 217

A lose conversation about automotive styling. What happened to the coachbuilders? Current design trends.

“Whatcha gonna do when the Gorgeous Ladies of Wrestling run wild on you?!?” Rob and Shane are back to talk about Netflix’s newest series; GLOW. Listen in, “If ya will”, as the guys give a thumbs up to “The Women of the … Continue reading →

Thanks to the support of you and our welfare checks, we've officially reached 200 hours of Podcasting on the Four and a Half Network! To celebrate this milestone - we've invited all of your favorite podcasters! This episode: Quinn Blakely (Good, Bad, and the Holy Sh*t show), Ryan Green ( Lost Sock Podcast), Thomas King (Lost Sock Podcast), Landry Miller (Laundry Room Show, Divine Heretics, Podcast Archives, Lost Sock Podcast), Cam Porter (Stylin & Profilin with Cam and Zam), Ethan Sandoval (Shenanigans, Podcast Archives), Joan Wright (Shenanigans), Michael Zampino (Opinions Like A-Holes, Stylin & Profilin with Cam and Zam) & Cian Baker (Lost Sock Podcast)Get a free audio book and 30-day free trial by going to audibletrial.com/archiveFor your free photo session when you purchase a wedding plan, go to TwoPlanetProductions.comThompson Cigar CompanyPurchase ANY cigars in this category and your ENTIRE order ships free! Receive Free Shipping on $99+ Orders! Save up to 73% when you purchase 5 Pack Fever Cigars! Listen on itunes Click here for your free audio book Click here

We're on location for the first time at Cobblehaus Brewing in the heart of Coraopolis, Pennsylvania. We sit down with Scott the head brewer and owner for a little chit chat, learn about Olde World beers and styles, and compete to see who knows the most about old beers in a Stylin' and Profilin' trivia showdown. Wooooo! Beers: Cobblehaus Old Towne Alt Bier Cobblehaus Porch Swing Dampfbier Cobblehaus Black Bear Black IPA   (Production Note: this episode was recorded in a room where beer was concurrently being brewed, so please understand the change in audio from our usual episodes.)

EPISODE 14 - This episodes features Katie Van Patten (Pillow Fight Podcast), Daren Ebacher (Stand up), Michael Zampino (Stylin' & Profilin' with Cam and Zam, and Opinions Like A-Holes). We answer questions from our listeners, talk to a bug enthusiast, AND play an Archived Podcast on our show.  If you want us to answer one of your questions, please leave us a review on iTunes and include it in your nice words about us. 

This episodes features Katie Van Patten (Pillow Fight Podcast), Daren Ebacher (Stand up), Michael Zampino (Stylin' & Profilin' with Cam and Zam, and Opinions Like A-Holes). We answer questions from our listeners, talk to a bug enthusiast, AND play an Archived Podcast on our show. If you want us to answer one of your questions, please leave us a review on iTunes and include it in your nice words about us.

Today: Lauren Turner and Michael Zampino come by to discuss The Grunge, do a stand up competition, and talk some comedy gossip! Lauren Turner is hosting at The Venue Shrine tomorrow night (4/30)and Zampino's new podcast, "Stylin and Profilin w/ Cam & Zam" releases next Friday (5/5)

In this week's episode, Bob explains what went wrong in Jesse Eldridge's trial. We fill in the gaps from the prosecution's case, break down the defense's case and strategy, and Bob reveals a critical error that may have cost Jesse his life. Today's Sponsors: ziprecruiter.com/truth - to try it for free stamps.com - click the microphone and enter code TRUTH for a special offer #hernamewaskeow #freejesse #justiceforjesse #realcrimeprofile

In this totally tubular episode, we kick off the new year with our return to the classic era of the TMNT over a mega slice of cheesesteak pizza! In addition to our Turtle pickups and news, we discuss: April's Antiquities – The 1989 Playmates Sewer Playset Casey's Comic Classroom – Volume 1, Issue 7 of the Mirage series (Released May, 1986) Green Screen – '87 Fred Wolf Cartoon Season 2, Episode 4 entitled “The Mean Machines” (Aired October 22nd, 1988) Special thanks to Thaddeus Manning for our news segments this week and his message for our Turtle Comm! Here's the news we cover: 1. TMNT Adventures Vol. 13 – 5/9/17 (Issues 55-57) 2. TMNT/Batman Adventures TPB – 7/18/17 3. “TMNT 100 Project” – 2/22/17 4. Stephen Amel to appear at Wizard World Comic Con in New Orleans Ernest Memorial Center – January 6-8 5. NECA Releases Leonardo Figure from 90s Film Another special slice of pizza goes out to Brian for his email and Rob V. for his drawing of Casey and the Turtles that is part of this episode's picture collage! Check out Brian's bodacious article on TMNT Cereal at: http://www.cluttermagazine.com/news/2016/05/teenage-mutant-ninja-turtles-cereal-comparison

Rod and Karen discuss some of Rod's random thoughts, Brandi and Countess Vaughn squash the beef, Martin Shkreli's twitter gets hacked, troop leader attacked by bear, landslide hits China, Nicki Mina, blind meditation, Sandra Bland, Emma Akpan, woman charged with murder trying to do her own abortion, Game of Thrones changes approach to rape, woman reports loud sex, Oberlin College, Thomas Gilbert Jr update, woman harasses coworker with confederate flag, Penn Supreme Court justice sends racist emails, Monet Bell on Black Love, school presentations that kept it real, Barack Obama highway, woman beats husband for farting, woman drives over people in Las Vegas, man snorts coke in pizza shop, father falls from the attic and sword ratchetness. Twitter: @rodimusprime @SayDatAgain @TBGWT Email: theblackguywhotips@gmail.com Blog: www.theblackguywhotips.com Voice Mail: 704-557-0186 Sponsors: www.GetBevel.Com Code: TBGWT

In this episode, the girls talk shawl styling, and many other things in this longer-than-usual episode. Contest To win a copy of the lovely View from the Charles shawl pattern by the wonderful Jen of the Down Cellar Studio podcast, post in the thread on the Ravelry group. Finished Projects Susie finished her Wild Goose Shawlette, and it is fabulous. She found it to be a super quick knit, and very very enjoyable. Danie finished her Box Troll Mittens, and two adorable mice. Current Projects Danie knit more on her Corrugated Box, her Fuzzy Hat, her third mouse, and her Equatorial Nights cowl. She has carefully planned out milestones to complete it for the Oh! Loops Harry Potter KAL 2015. Susie worked a lot on her Rainbow Connection Socks, and a little on her Anemone shawl. She cast on a Candy Cane Stocking using Valley Yarns Northampton Sport in Ocean Heather and Natural, and a Glenfiddich sweater in Miss Babs Yowza in Cloak. Her best-laid fitting plans ran into a son of a stitch, but she’s re-plotted her course. Review Danie reviews Sun Valley Fiber‘s Yarn Buddy. New to Me Susie has been enjoying the audio podcast Skein Enable and the video podcast Stitched in Sweden. Project Reconsideration Susie reconsiders her Gridelin and her Aranami shawl.

Hosts Bryan McGuckin, Chris Sorrentino and John Szeluga share a few drinks while discussing birthdays, the recent leap second, Top Gear, riding the bus, Dr. Strange, Bud-blood, being short, the Heimlich Maneuver, Breakfast at Tiffany's, The A-Team, the Ghostbusters reboot, women in film, The Day the Clown Cried, Barbara Walters' interview with the Teenage Mutant Ninja Turtles, Sesame Street, Steampipe Alley, vintage toys, Big Trouble in Little China, Rick Flair, Fair Game (1995), Sharon Stone, Spider-Man, and more! www.SuperLiveAdventure.com

Oh this weeks show we review WWE's "Fastlane" PPV, Preview ROH's 13th anniversary PPV , Dave makes his selections for the New Japan Cup contest brackets, "Maniac" Matt calls in from his hospital bed recovering from the Rant's Black Achiever Awards Show and we wrap up black history month with a look back at the career of "Sweet" Daddy Siki

Beyond The Bell is “Stylin and Profilin” with the Horsemen Files: The History of the Four Horsemen. @SeanBeckerman breaks down the year 1988 as the Horsemen recruit their newest member Barry Windham! Get ready to...

#BeyondTheBell is “Stylin and Profilin” with the Horsemen Files: The History of the Four Horsemen. @SeanBeckerman breaks down the year 1989 as the Horsemen are reborn. JJ leaves, the Anderson’s return and they partner with...

Beyond The Bell is “Stylin and Profilin” as the Horsemen Files returns! In celebration of their induction into the 2012 WWE Hall of Fame, Beyond The Bell opens up the vault to the Horsemen Files:...

#BeyondTheBell is “Stylin and Profilin” with the Horsemen Files: The History of the Four Horsemen. @SeanBeckerman breaks down the year 1989 as the Horsemen are […]

#BeyondTheBell is “Stylin and Profilin” with the Horsemen Files: The History of the Four Horsemen. @SeanBeckerman breaks down the year 1989 as the Horsemen are […]

Beyond The Bell is “Stylin and Profilin” with the Horsemen Files: The History of the Four Horsemen. @SeanBeckerman breaks down the year 1988 as the […]

Join host, Garrett Miller, in welcoming STYLE TO THE STARS Guru George Brescia of George B Style!  George will get you Stylin' and Profilin' in no time flat!  Call in:  323 657-1493!  7:00-8:00 pm PST, Wednesday!

Join host, Garrett Miller, in welcoming STYLE TO THE STARS Guru George Brescia of George B Style!  George will get you Stylin' and Profilin' in no time flat!  Call in:  323 657-1493!  7:00-8:00 pm PST, Wednesday!

Join host, Garrett Miller, in welcoming STYLE TO THE STARS Guru George Brescia of George B Style!  George will get you Stylin' and Profilin' in no time flat!  Call in:  323 657-1493!  7:00-8:00 pm PST, Wednesday!

Join host, Garrett Miller, in welcoming STYLE TO THE STARS Guru George Brescia of George B Style!  George will get you Stylin' and Profilin' in no time flat!  Call in:  323 657-1493!  7:00-8:00 pm PST, Wednesday!

Join host, Garrett Miller, who welcomes the Stylist to the Start: George B Style (George Brescia).  George will get you Stylin' & Profilin'!  Call in:  323 657-1493!  7:00-8:00 pm PST, Wednesday night!

Join host, Garrett Miller, who welcomes the Stylist to the Start: George B Style (George Brescia).  George will get you Stylin' & Profilin'!  Call in:  323 657-1493!  7:00-8:00 pm PST, Wednesday night!

Beyond The Bell is “Stylin and Profilin” as the Horsemen Files returns! In celebration of their induction into the 2012 WWE Hall of Fame, Beyond […]

Beyond The Bell celebrates the 50th edition with some “Stylin and Profilin” as the Horsemen Files returns! In celebration of their induction into the 2012 […]

Beyond The Bell celebrates the 50th edition with some “Stylin and Profilin” as the Horsemen Files returns! In celebration of their induction into the 2012 WWE Hall of Fame, Beyond The Bell opens up the...

Tricky Moreira’s fifty minute live mix on This House Radio

Jared talks about his trip to the Star Trek Convention in Parsipanny, NJ fights with a WAWA manager and is just generally pissed off.

Jared talks about his trip to the Star Trek Convention in Parsipanny, NJ fights with a WAWA manager and is just generally pissed off.

Die akute Pankreatitis beginnt in den exokrinen Azinuszellen des Pankreas und wird durch verschiedene, bisher nicht vollständig geklärte, intrazelluläre Vorgänge ausgelöst. Das Hormon Cholezystokinin stimuliert Signaltransduktionskaskaden, welche über eine Reorganisation des Aktinzytoskeletts zu einer akuten Organentzündung führen. In dieser Arbeit wurde untersucht, ob ein über das Enzym RhoA vermittelter intrazellulärer Signalweg zu Aktin-bindenden Proteinen im Pankreas diese Reaktion hervorruft und durch Cholezystokinin reguliert werden kann. Die Ergebnisse der vorliegenden Arbeit bringen den Nachweis der Existenz der im Folgenden beschriebenen Signaltransduktionskaskade in exokrinen Azinuszellen: RhoA führt über eine Aktivierung von ROCK II zu einer Phosphorylierung der Zieluntereinheit MYPT1 der Myosinphosphatase und somit zu einer Hemmung des Gesamtenzyms. Dadurch transloziert die sowohl in der Zytosol- als auch in der Zytoskelettfraktion vorkommende, unphosphorylierte Form MYPT1 vollständig ins Zytosol. Die Myosinphosphatase führt zu einer Dephosphorylierung der MLC von Myosin. Die fast vollständig in der Zytoskelettfraktion exprimierte phosphorylierte Form pMLC transloziert im dephosphorylierten Zustand ins Zytosol. Durch die Interaktion mit MYPT1 kann MLC zu einer Aktinmyosinkontraktion und somit zu einer Reorganisation des Aktinzytoskeletts führen. Über alternative Signalwege bewirkt RhoA eine Aktivierung von mDia, welches mittels Profilin zu einer Aktinpolymerisation führt. Über ROCK II wird eine Aktivierung der LIMK durch RhoA vermittelt. Dadurch wird Cofilin vermehrt phosphoryliert, wodurch die Depolymerisation der Aktinfilamente gehemmt wird. Durch eine dosis- und zeitabhängige Stimulation mit physiologischen und supraphysiologischen Dosierungen Cholezystokinin wird der Signalweg über RhoA gehemmt. Dadurch kann eine Kontraktion des Aktinzytoskeletts stattfinden und es zu einer Fusion von Vesikeln und zu einer Inhibierung des regulären Sekretionsmechanismus der Pankreaszellen kommen. Da die Hemmung von Aktin-modulierenden Proteinen eine bedeutende Rolle bei der Organfunktion und Entwicklung der akuten Pankreatitis spielt, trägt diese Arbeit dazu bei, sowohl die physiologischen als auch die pathophysiologischen Vorgänge innerhalb der Azinuszellen näher zu charakterisieren. Dies könnte zu einem besseren Verständnis der dieser Erkrankung zugrundeliegenden Mechanismen führen und somit einen therapeutischen Ansatz bei der akuten Pankreatitis darstellen.

Wed, 12 Sep 2007 12:00:00 +0100 https://edoc.ub.uni-muenchen.de/7427/ https://edoc.ub.uni-muenchen.de/7427/1/Shivalkar_Madhuri.pdf Shivalkar, Madhuri

The major goal of the project was the investigation of proteins that regulate dynamic rearrangements of the actin cytoskeleton in Dictyostelium discoideum amoebae. Among the proteins studied in detail were (i) D. discoideum vasodilator stimulated phosphoprotein DdVASP and a new profilin isoform as putative regulators of filopodia formation, and (ii) the Ste20-like kinases Krs1 and Severin-Kinase as members of signalling cascades towards the actin cytoskeleton. Filopodia are bundles of actin filaments projecting from the cell surface. They are found on a variety of cell types and are needed among others, for cell adhesion, sensory and exploratory functions. Filopodia are frequently found associated with sheet-like arrays of actin filaments called lamellipodia and membrane ruffles. The function of the VASP homolog from D. discoideum in filopodia formation was studied using molecular, biochemical and cell biological approaches. The protein sequence of DdVASP shares a significant homology to the members from other species. The protein harbours two Ena/VASP homology domains EVH1 and EVH2 separated by a polyproline stretch. The EVH2 domain is characterised by a G-actin binding site (GAB), an F-actin binding site (FAB) and a tetramerisation domain. As a tetramer the DdVASP protein can nucleate actin polymerization and bundle actin filaments. The in vitro nucleating activity of DdVASP is salt dependent and its nucleating activity is completely abolished at 100 mM salt. However, the F-actin bundling activity as determined by the low speed sedimentation assay was not disturbed. The ability of DdVASP to influence the binding of capping protein to the growing ends of the actin filaments was tested through elongation of capped F-actin seeds and by depolymerization of capped filaments upon dilution below the critical concentration of the barbed ends. Results from both sets of experiments showed that DdVASP cannot remove capping protein from the barbed ends. The D. discoideum formin dDia2, which was previously reported to be essential for filopodia formation could elongate the capped F-actin seeds. In vitro biochemical data led to the conclusion that the bundling activity of DdVASP is the essential in vivo function to stabilise actin filaments in emerging filopodia. To test this hypothesis, a DdVASP null mutant was isolated. As expected the mutant failed to produce any filopodia. Expression of wild type DdVASP, but not DdVASPFAB, rescued the phenotype suggesting the importance of the bundling activity of DdVASP in filopodia formation. To confirm that the data obtained with DdVASP were not species specific, key biochemical functions of HsVASP were also tested. The results indicated that VASPs are functionally well conserved throughout evolution. During this study, a third profilin isoform, profilin III, was further characterised. Specific interaction between profilin III and DdVASP was discovered. Profilin III shares a limited homology at the amino acid level with the other two and well studied profilins. Polyclonal antibodies that recognise only the profilin III isoform showed that in wild type cells profilin III represents less than 1% of all profilins. This suggests a distinct role for profilin III, because a low protein concentration argues against an actin sequestering function. Immunolocalisation studies showed profilin III in filopodia and enriched at their tips. Cells lacking the profilin III protein show defects in cell motility during chemotaxis. The second part of the project dealt with the characterisation of two D. discoideum Germinal Centre Kinases (GCK). The catalytic domain of Krs1 was found to be highly homologous to the catalytic region of human MST1 and MST2 from the GCK-II subfamily. The regulatory region harbours the putative inhibitory domain (aa 330-379) and a possible multimerization (SARAH) domain (aa 412-458) described for GCKs in higher organisms. This SARAH region spans about 50 amino acid residues, is located at the extreme carboxyl terminus and most likely forms an  - helical coiled-coil motif. GFP-Krs1 overexpressing wild type cells showed an enrichment of the kinase in the cell cortex, and motility of these cells during aggregation was reduced. Krs1 knockout strains exhibited only subtle differences to wild type cells. Severin kinase is encoded by the gene svkA, and phylogenetic analysis groups it into subfamily GCK-III, along with human MST3, MST4 and YSK/SOK-1. Immunoblot analysis with polyclonal antibodies showed an uniform expression level throughout development. Gene disruption of svkA resulted in cells that had problems to divide both in submerged or in shaking cultures. Though the motility and chemotaxis of these cells remain unaltered compared to the wild type cells, the movement of the multicellular slugs is disturbed. In addition, development was delayed and the mutant formed aberrant fruiting bodies.

Die vorliegende Arbeit hatte zum Ziel, (i) die regulatorische Funktion bestimmter integraler Membranproteine im Dictyostelium Zytoskelett und (ii) die biochemische Interaktion einer Kinase eines infektiösen Bakteriums mit Aktin aus der Wirtszelle genauer zu analysieren. (i) Die ubiquitären Mitglieder der CD36/LIMPII-Familie sind integrale Membranproteine, die als Lipidrezeptoren und Zelladhäsionsproteine in der Plasmamembran oder - mit bisher unbekannter Funktion - in Membranen endosomaler Vesikel vorkommen. In Dictyostelium discoideum führte die Inaktivierung eines lysosomalen Membranproteins aus dieser Gruppe zur Suppression des Phänotyps einer Profilin-minus Mutante. Im Zuge der vollständigen Sequenzierung des D. discoideum-Genoms konnte festgestellt werden, daß es neben diesem DdLmpA noch die beiden weiteren homologen Proteine DdLmpB und DdLmpC gibt. Da der Mechanismus der Suppression des Profilin-minus Phänotyps ungeklärt ist, wurden die beiden Isoformen im Rahmen der vorliegenden Arbeit genauer charakterisiert. Sowohl für DdLmpB wie auch für DdLmpC konnte die familientypische Membran-Topologie einer Haarnadelstruktur nachgewiesen werden. Dabei weist die zentrale, lumenale Domäne beider Proteine zahlreiche Glykosylierungen auf. Durch Immunofluoreszenz und Saccharosegradienten wurde die Lokalisation der drei Isoformen an endolysosomalen Vesikeln nachgewiesen. Es stellte sich dabei heraus, daß die drei DdLmp-Proteine in unterschiedlichen Vesikelpopulationen auftraten. Auch “pulse-chase“-Experimente mit TRITC-Dextran und nachfolgender Markierung der Vesikel mit DdLmp-spezifischen Antikörpern ergaben unterscheidbare Zeitmuster für die Rekrutierung der Membranproteine in Vesikeln. Die für DdLmpA oft beobachtete Kolokalisation mit Makropinosomen konnte z.B. für DdLmpB und DdLmpC nur selten festgestellt werden. Nach zahlreichen Versuchen und der Konstruktion von verschiedenen Vektoren konnte am Ende der praktischen Arbeiten eine DdLmpB-minus Mutante im Wildtyp-Hintergrund isoliert werden. (ii) Im zweiten Teil der Arbeit wurde die in der Literatur beschriebene Interaktion zwischen Aktin und der Kinase YopO, die durch Yersinia enterocolitica als Effektorprotein in die Wirtszelle transloziert wird, biochemisch genauer untersucht. Es konnte festgestellt werden, daß G-Aktin und nicht F-Aktin für die Aktiverung der YopO-Kinase verantwortlich ist. Dabei tritt Nichtmuskel-Aktin im Vergleich zum Muskel-Aktin als ein deutlich besserer Aktivator von YopO auf. Obwohl die aktivierte Kinase in vivo das Aktin-Zytoskelett beeinflußt, ist Aktin offensichtlich kein Substrat von YopO. Mittels Fluoreszenzspektroskopie konnte gezeigt werden, daß sowohl die native Kinase YopO als auch das durch Punktmutation inaktivierte YopO K269A die Polymerisierungskinetik von Aktin behindern. Für eine mutmaßliche Aktin-Binderegion von 20 Aminosäuren aus dem C-terminalen Ende konnte hingegen kein Effekt beobachtet werden. Der Einfluß von aktinbindenden Proteinen, aktinmodifizierenden Substanzen und YopO-bindenden GTPasen auf die Aktivierung der Kinase durch Aktin deutet darauf hin, dass die Aktivität der Kinase in der Wirtszelle nicht nur durch Aktin alleine, sondern auch durch weitere Zytoskelett-Komponenten reguliert wird.

A large number of actin-binding proteins regulates the dynamics of the actin cytoskeleton. Here we report the identification and characterization of two new proteins in Dictyostelium discoideum: the novel cytoskeleton protein villidin and a third profilin isoform. One goal of the project was to analyze the features of the villidin sequence in detail and to investigate the protein by molecular, biochemical and cell biological approaches. Villidin has a calculated molecular mass of 190,000 Da. Based on the domain structure of the protein, villidin can be assigned to the gelsolin/villin-family as well as to the WD-repeat family. In principal, the group of the WD-repeat proteins includes regulatory proteins which are involved in signal transduction and other important cellular processes. The N-terminus of villidin harbours between four and eight of the specific WD-repeats and forms probably a β-propeller structure. The WD-domain is followed by an intervening domain of 400 amino acids that leads to the second characteristic villidin domain at the C-terminus. This part of the sequence exhibits a similarity to villin, though the first of the six villin domains is absent in villidin. However the typical headpiece is present. Villidin mRNA and protein are expressed in low amounts during growth and early aggregation, they are increased during development and reach highest levels at the tipped aggregate stage. The protein is present in the cytosol as well as in the cytoskeletal and the membrane fraction. These biochemical results are in agreement with the immunofluorescence data. The endogenous villidin is homogeneously distributed throughout the cytosol and localizes at vesicular structures. Colocalization experiments lead to the assumption that these structures might belong to a still unknown population of vesicles. GFP fusion proteins with the villin homology domains show a similar distribution, whereas GFP fusions of the N-terminal part encompassing the WD-repeats are present in F-actin rich regions at the plasma membrane and on internal membranes during motility, pinocytosis and phagocytosis. Mutants lacking villidin do not show an aberrant phenotype during growth and development but are defective in motility and phototaxis in the multicellular slug stage. Based on this defect and the multidomain structure of the protein, villidin might be involved in signal transduction processes leading to phototactic movement. The second part of the project dealt with a new gene which codes for a third profilin isoform in D. discoideum. The well-known Dictyostelium profilin isoforms I and II are able to interact with G-actin, PIP2 and poly-L-prolin. As in the case of profilin I and II, profilin III is encoded by a single gene. In contrast to profilin I and II, the transcription of profilin III is not developmentally regulated. All three isoforms show the typical limited homology at the amino acid level. The recombinant Profilin III protein cosediments with poly-L-prolin, inhibits the actin-polymerisation and the PIP2 interaction of profilin III competes with the G-actin affinity. The low expression of Profilin III mRNA in growing and developing cells suggests a distinct role of profilin III because a low protein concentration argues against an actin sequestering function.

Profilin is an ubiquitous cytoskeletal protein whose function is fundamental to the maintenance of normal cellular physiology. Site-directed mutagenesis of profilin II from Dictyostelium discoideum by PCR resulted in the point mutations W3N and K114E, whereby the W3N profilin is no longer able to bind to poly-(L)-proline concomitant with a slight reduction in actin-binding, whereas the K114E profilin shows profound decrease in its ability to interact with actin but its affinity for poly-(L)-proline remained unaltered. The in vivo properties of the point-mutated profilins were studied by expressing either W3N or K114E in the profilin-minus D. discoideum mutants which have defects in the F-actin content, cytokinesis and development (Haugwitz et al., 1994). Expression resulted in normal cell physiology, a reduction in the F-actin content, and a complete development. Interestingly, only cells which overexpressed W3N could restore the aberrant phenotype, while the K114E profilin with its fully functional poly-(L)-proline binding and its strongly reduced actinbinding activities rescued the phenotype at low concentrations. Both the wild-type and pointmutated profilins are enriched in phagocytic cups during uptake of yeast particles. These data suggest a) that a functional poly-(L)-proline binding activity is more important for suppression of the mutant phenotype than the G-actin binding activity of profilin, and b) that the enrichment of profilin in highly active phagocytic cups might be independent of either poly-(L)-proline or actin-binding activities. To have a better understanding of the in vivo role of profilin, D. discoideum profilin II has been tagged at its C-terminus with the green fluorescent protein (GFP) with a 100-aa linker separating profilin and GFP. This fusion construct was introduced in D. discoideum profilinminus cells and expression of the fusion protein could restore the aberrant phenotype partially. The partial rescue might be due to the uneven expression of the fusion protein leading to mixed populations even after repeated recloning. The profilin-GFP transformants showed normal cell morphology, could be cultivated in shaking suspensions, and could develop fruiting bodies which closely resembled those of the wild-type. In vivo studies revealed the distribution of the fusion protein in highly active regions of the cells such as phagocytic cups, macropinocytotic crowns, cell cortex and at the leading edges of locomoting cells. Thus profilin appears to play a significant role in the regulation of the dynamic actinbased cellular processes. A second actin-regulatory protein from D. discoideum namely, severin, a Ca2+-dependent Factin fragmenting and capping protein, was also investigated via fusion to GFP at its C-terminus. Although severin is a very active F-actin fragmenting protein in in vitro assays, the severin null D. discoideum mutant exhibits normal growth, cell motility, chemotaxis and development. Examination of the live dynamics of severin-GFP should clarify the in vivo role of severin and other functionally redundant cytoskeletal proteins. The 70 kDa severin-GFP fusion protein has been sufficiently expressed and partially purified from the severin null cells whereby in vitro assays confirmed the ability of this fusion protein to sever F-actin only in the presence of Ca2+. Data from confocal microscopy showed that the fusion protein was transiently detected in macropinocytotic crowns, phagocytic cups, membrane ruffles, at the leading edges of motile cells and cell-cell contacts of aggregating cells in directed motion. These data suggest an in vivo role for severin in the remodulation of existing F-actin structures as supported by the in vitro data. The highly dynamic cytoskeleton also plays a significant part in the defence of the cells against pathogens. The behaviour of the actin cytoskeleton of cultured mammalian cells in response to Yersinia enterocolitica infection was examined by confocal microscopy with the aid of GFP-tagged actin, cofilin and profilin II. The translocated Yersinia outer proteins (Yops) encoded by a virulence plasmid in the wild-type bacteria have been observed to disrupt the actin microfilaments, resulting in diffuse actin staining which subsequently disappeared completely upon prolonged bacterial infection. In addition, F-actin structures resembling phagocytic cups were found at the sites of bacterial adherence, suggesting the likelihood of the involvement of the Rho family of small GTPases in the regulation of the actin cytoskeleton. The secreted Yops appeared to have no major effect on the distribution of GFP-profilin whereas the staining pattern of GFP-cofilin seemed to be modified by the Yops, resulting in a decrease in length of the actin-cofilin rods and a diffuse localization of cofilin. The exact mechanisms of interaction between the Yops and their host targets remain to be determined. However, a clearer insight into the interaction between pathogens and the host cytoskeleton will certainly aid in the cellular defence and the prevention of pathogenesis.

Fri, 1 Jan 1988 12:00:00 +0100 http://epub.ub.uni-muenchen.de/8579/ http://epub.ub.uni-muenchen.de/8579/1/Mueller_Guenter_8579.pdf Magdolen, Viktor; Oechsner, Ulrich; Müller, Günter; Bandlow, Wolfhard Magdolen, Viktor; Oechsner, Ulrich; Müller, Günter und Bandlow, Wolfhard (1988): The intron-containing gene for yeast profilin (PFY) encodes a vital function. In: Molecular and Cellular Biology, Vol. 8, Nr. 12: pp. 5108-5115. Chemie und Phar