Podcasts about ck2

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.05.05.539436v1?rss=1 Authors: Smythe, E., Alshahrani, F., Zhu, Z., Ferreira, F., Maib, H., Pruzina, S., Robinson, D., Murray, D. H., McComb, A. Abstract: Epidermal growth factor receptor (EGFR) signalling results in a variety of cell behaviours, including cell proliferation, migration and apoptosis, which depend on cell context. Here we have explored how the Rab5GEF, Rme-6, regulates EGFR signalling by modulating endocytic flux. We demonstrate that Rme-6, which acts early in the endocytic pathway, regulates EGFR trafficking through an endocytic compartment that is competent for ERK1/2 signalling. While overexpression of Rme-6 results in enhanced ERK1/2 nuclear localisation and c-Fos activation, loss of Rme-6 results in aberrant ERK1/2 signalling with increased cytoplasmic ERK1/2 phosphorylation (Thr202/Tyr204) but decreased ERK1/2 nuclear translocation and c-Fos activation, the latter leading to decreased cell proliferation. Phosphorylation of ERK1/2 by protein kinase 2 (CK2) is required for its nuclear translocation and our data support a model whereby Rme-6 provides a scaffold for a population of CK2 which is required for efficient nuclear translocation of ERK1/2. Rme-6 is itself a substrate for CK2 on Thr642 and Ser996 and phosphorylation on these sites can activate its Rab5GEF activity and endocytic trafficking of EGFR. Together our results indicate that that Rme-6 co-ordinates EGFR trafficking and signalling to regulate the assembly and disassembly of an ERK1/2 signalosome. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.04.11.536369v1?rss=1 Authors: Wang, C.-Y., Zuo, Z., Kim, K., Bellen, H. J., Lee, H. K. Abstract: Wnt signaling plays an essential role in developmental and regenerative myelination in the CNS. The Wnt signaling pathway is comprised of multiple regulatory layers; thus, how these processes are coordinated to orchestrate oligodendrocyte development remains unclear. Here we show CK2, a Wnt/{beta}-catenin signaling Ser/Thr kinase, phosphorylates Daam2, inhibiting its function and Wnt-activity during oligodendrocyte development. Intriguingly, we found Daam2 phosphorylation differentially impacts distinct stages of oligodendrocyte development, accelerating early differentiation followed by decelerating maturation and myelination. Application towards white matter injury revealed CK2-mediated Daam2 phosphorylation plays a protective role for developmental and behavioral recovery after neonatal hypoxia, while promoting myelin repair following adult demyelination. Together, our findings identify a novel regulatory node in the Wnt pathway that regulates oligodendrocyte development via protein phosphorylation-induced signaling complex instability and highlights a new biological mechanism for myelin restoration. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.02.27.530364v1?rss=1 Authors: Berkane, R., Ho-Xuan, H., Glogger, M., Sanz-Martinez, P., Cano-Franco, S., Juretschke, T., Gonzales Cardenas, A., Glaesner, T., Beli, P., Husnjak, K., Doetsch, V., Grumati, P., Heilemann, M., Stolz, A. Abstract: Selective autophagy of the ER (ERphagy) is an important regulator of ER remodeling and critical to maintain cellular homeostasis upon environmental changes. ERphagy receptors link the ER with autophagic membrane thus regulating ERphagy flux. We recently showed that members of the FAM134 family play overlapping and distinct roles during stress-induced ERphagy. Yet the mechanisms on how they are activated remain largely unknown. In this study we analyzed mTOR-mediated dynamic phosphorylation of FAM134 as a trigger of FAM134-driven ERphagy. An unbiased screen of kinase inhibitors revealed CK2 to be essential for FAM134B- and FAM134C-driven ERphagy upon mTOR inhibition. Identified dynamic phosphorylation sites on FAM134C in cells were fitting with predicted CK2 targeting sites, indicating a direct regulatory role of CK2 in FAM134-driven ERphagy. Using super-resolution microscopy, we showed that activity of CK2 is essential for the formation of high-density clusters of FAM134B and FAM134C. Consistently, FAM134B and FAM134C proteins carrying point mutations of selected Serin residues, within their reticulon homology domain, are unable to form high-density clusters. In addition, we provide evidence that the ubiquitination machinery is required for ERphagy and that FAM134B and FAM134C clustering is activated by phospho-dependent ubiquitination. Treatment with CK2 inhibitor SGC-CK2-1 prevents Torin1-induced ERphagy flux as well as ubiquitination of FAM134 proteins and consistently, treatment with E1 inhibitor suppresses Torin1-induced ERphagy flux. Therefore, we propose CK2 dependent phosphorylation of ERphagy receptors precedes ubiquitin-dependent ERphagy flux activation. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Dr. Al Grauer hosts. Dr. Albert D. Grauer ( @Nmcanopus ) is an observational asteroid hunting astronomer. Dr. Grauer retired from the University of Arkansas at Little Rock in 2006. travelersinthenight.org Today's 2 topics: - Greg Leonard discovered C/2021 A1 (Leonard) in the constellation Canes Venatici. - Richard Kowalski 750' diameter asteroid 2021 CK2 while sitting on his living room couch!    We've added a new way to donate to 365 Days of Astronomy to support editing, hosting, and production costs.  Just visit: https://www.patreon.com/365DaysOfAstronomy and donate as much as you can! Share the podcast with your friends and send the Patreon link to them too!  Every bit helps! Thank you! ------------------------------------ Do go visit http://www.redbubble.com/people/CosmoQuestX/shop for cool Astronomy Cast and CosmoQuest t-shirts, coffee mugs and other awesomeness! http://cosmoquest.org/Donate This show is made possible through your donations.  Thank you! (Haven't donated? It's not too late! Just click!) ------------------------------------ The 365 Days of Astronomy Podcast is produced by the Planetary Science Institute. http://www.psi.edu Visit us on the web at 365DaysOfAstronomy.org or email us at info@365DaysOfAstronomy.org.

Part two of our look at cancer research at VA includes a conversation with Dr. Khalil Ahmend, Professor of Laboratory Medicine and Pathology at the University of Minnesota. Dr. Ahmed has been a Senior Research Career Scientist, Minneapolis VA since 1978. For over forty years, Dr. Ahmed's long-standing interest has been in protein CK2 (formerly casein kinase 2 or II). He investigation of its therapeutic targeting for cancer therapy and how treatments might be effective for stopping progression to resistant growth or improving response setting the stage for potential ground-breaking translation into the clinic.

Welcome to the Paradox Podcast! Paradox Interactive podcast about the business of video games where try to explain why we do the stuff we do.Today, Shams and Daniel, discuss 2 equally horrifying scenarios:What happens when we screw up a game release?Possibly worse – what are the implications of a greatly successful game launch?Timestamps:0:15 – Introduction & excuses tbh.4:38 – Why can't everything be Valheim?8:26 – CK3's success & its consequences.19:09 – What happens when a game release don't go well?27:45 – The value of taking risks33:13 – Wrapping upYou can also find today's episode on:• Spotify: https://pdxint.at/2HFshtu​• iTunes: http://pdxint.at/2BnBWBo​• Player.fm: http://pdxint.at/2EWTCnl​• Poddtoppen: http://pdxint.at/2CiqXGw​• Acast: http://pdxint.at/2ExBD9lWelcome​ Thanks for listening, and see you in the next episode!Paradox on YouTube:---------------------------------ParadoxInteractive - http://youtube.com/ParadoxInteractiveTrailers, Feature Breakdowns, Dev Diaries, and more.ParadoxExtra - http://youtube.com/ParadoxExtraGameplay of our video games, convention coverage, and other fun things from Paradox!Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategyGameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and Imperator.Support the show (http://paradoxplaza.com)

charity worth it?where do you see yourself in 5 years?how do you feel about marriage?cost of livingtax and living abroadwow addictionnew pcck2 game of thrones modCk3new year's resolutionsworking too muchlong term relationships OR better off single?empire of sin good game?pdxcon and consnew camhair transplant

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.29.359380v1?rss=1 Authors: Yu, D., Zarate, N., Cuccu, F., Yue, J. S., Brown, T. G., Tsai, W., Mansky, R., Jiang, K., Kim, H., Nanclares, C., Nichols-Meade, T., Larson, S. N., Gundry, K., Zhang, Y., Benneyworth, M., Öz, G., Cvetanovic, M., Araque, A., Gomez-Pastor, R. Abstract: Huntington's Disease (HD) is a neurodegenerative disorder caused a polyglutamine expansion in the HTT protein. This mutation causes HTT misfolding and aggregation, preferentially affecting neurons of the basal ganglia. Other aggregation-prone proteins like alpha-synuclein (-syn), mostly associated with Parkinson's disease (PD), has recently been involved in motor deficits in HD, but its mechanism of action is unknown. Here we showed that -syn serine 129 phosphorylation (-syn-pS129), a posttranslational modification linked to -synucleinopathy, is highly phosphorylated in the brain of symptomatic zQ175 HD mice. We demonstrated that such phosphorylation is mediated by Protein Kinase CK2 alpha prime (CK2'), which is preferentially induced in striatal neurons in HD. Knocking out one allele of CK2' in zQ175 mice decreased -syn-pS129 in the striatum and ameliorated several HD-like symptoms including neuroinflammation, transcriptional alterations, excitatory synaptic transmission dysfunction and motor deficits. Our data suggests CK2'-mediated synucleinopathy as a key molecular mechanism for striatal neurons degeneration in HD. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.20.258863v1?rss=1 Authors: Barak-Broner, N., Singer-Lahat, D., Chikvashvili, D., Lotan, I. Abstract: The polybasic juxtamembrane region (5RK) of the plasma membrane neuronal SNARE, syntaxin1A (Syx), was shown by us to act as a fusion clamp in PC12 cells, making release dependent on stimulation by Ca2+. By using a Syx-based FRET probe, we demonstrated that 5RK is absolutely required for a depolarization-induced Ca+2-dependent, close-to-open transition (CDO) of Syx that involves the vesicular SNARE synaptobrevin2 and occurs concomitantly with Ca2+-triggered release. Here, we investigated the mechanism underlying the 5RK requirement, and identified phosphorylation of Syx at Ser-14 (S14) by protein kinase CK2 as a crucial molecular determinant. Following biochemical verification that both endogenous Syx and CSYS are constitutively S14 phosphorylated in PC12 cells, dynamic FRET analysis of phospho-null and phospho-mimetic mutants of CSYS and the use of a CK2 inhibitor revealed that it is the S14 phosphorylation that confers the 5RK requirement. Concomitant amperometric analysis of catecholamine release revealed that the phospho-null mutants do not support release, spontaneous and evoked. Collectively, these results identify a functionally important CK2 phosphorylation site in Syx that is required for 5RK-regulation of CDO and for concomitant Ca2+-triggered release. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.06.12.148825v1?rss=1 Authors: Cai, Y. D., Xue, Y., Truong, C., Del-Carmen Li, J., Ochoa, C., Vanselow, J. T., Murphy, K. A., Li, Y. H., Liu, X., Kunimoto, B. L., Zheng, H., Zhao, C., Zhang, Y., Schlosser, A., Chiu, J. C. Abstract: Circadian clocks orchestrate daily rhythms in organismal physiology and behavior to promote optimal performance and fitness. In Drosophila, key pacemaker proteins PERIOD (PER) and TIMELESS (TIM) are progressively phosphorylated to perform phase-specific functions. Whereas PER phosphorylation has been extensively studied, systematic analysis of site-specific TIM phosphorylation is lacking. Here, we identified phosphorylation sites of PER-bound TIM by mass spectrometry, given the importance of TIM as a modulator of PER function in the oscillator. Among the twelve TIM phosphorylation sites we identified, at least two of them are critical for circadian timekeeping as mutants expressing non-phosphorylatable mutations exhibit altered behavioral rhythms. In particular, we observed that CK2-dependent phosphorylation of TIM(S1404) promotes nuclear accumulation of PER-TIM heterodimers by inhibiting the interaction of TIM and nuclear export component, Exportin 1 (XPO1). We postulate that proper level of nuclear PER-TIM accumulation is necessary to facilitate kinase recruitment for the regulation of daily phosphorylation rhythm and phase-specific transcriptional activity of CLOCK (CLK). Our results highlight the contribution of phosphorylation-dependent nuclear export of PER-TIM heterodimers to the maintenance of circadian periodicity and identify a new mechanism by which the negative elements of the circadian oscillator (PER-TIM) regulate the positive elements (CLK-CYC). Finally, since the molecular phenotype of tim(S1404A) non-phosphorylatable mutant exhibits remarkable similarity to that of a mutation in human timeless that underlies Familial Advanced Sleep Phase Syndrome (FASPS), our results revealed an unexpected parallel between the functions of Drosophila and human TIM and may provide new insights into the molecular mechanisms underlying human FASPS. Copy rights belong to original authors. Visit the link for more info

Just  some very NSFW banter during a typical CK2 game. For those who     don't  know Crusader Kings 2 is a dynasty simulator where players   create  a   family in medival europe and try to survive and rise in the   ranks of    great houses of europe. Taken out of context some of the   topics    discussed are quite jarring to the sensibilities. I assume if   you are    listening to this you already know all of this. We are  doing a     megacampaign spanning from the easiest start date in CK2  through to   the   end then we are going to convert it for Europa  Universalis 4 and   play  it  through to the end of that game, basically  a a victory lap   before  CK3  comes out to celebrate which is in my  opinion a perfect   game.

Just  some very NSFW banter during a typical CK2 game. For those who    don't  know Crusader Kings 2 is a dynasty simulator where players  create  a   family in medival europe and try to survive and rise in the  ranks of    great houses of europe. Taken out of context some of the  topics    discussed are quite jarring to the sensibilities. I assume if  you are    listening to this you already know all of this. We are doing a     megacampaign spanning from the easiest start date in CK2 through to   the   end then we are going to convert it for Europa Universalis 4 and   play  it  through to the end of that game, basically a a victory lap   before  CK3  comes out to celebrate which is in my opinion a perfect   game.

Just  some very NSFW banter during a typical CK2 game. For those who   don't  know Crusader Kings 2 is a dynasty simulator where players create  a   family in medival europe and try to survive and rise in the ranks of    great houses of europe. Taken out of context some of the topics    discussed are quite jarring to the sensibilities. I assume if you are    listening to this you already know all of this. We are doing a    megacampaign spanning from the easiest start date in CK2 through to  the   end then we are going to convert it for Europa Universalis 4 and  play  it  through to the end of that game, basically a a victory lap  before  CK3  comes out to celebrate which is in my opinion a perfect  game.

Just  some very NSFW banter during a typical CK2 game. For those who   don't  know Crusader Kings 2 is a dynasty simulator where players create  a   family in medival europe and try to survive and rise in the ranks of    great houses of europe. Taken out of context some of the topics    discussed are quite jarring to the sensibilities. I assume if you are    listening to this you already know all of this. We are doing a    megacampaign spanning from the easiest start date in CK2 through to  the   end then we are going to convert it for Europa Universalis 4 and  play  it  through to the end of that game, basically a a victory lap  before  CK3  comes out to celebrate which is in my opinion a perfect  game.

Just some very NSFW banter during a typical CK2 game. For those who   don't know Crusader Kings 2 is a dynasty simulator where players create  a  family in medival europe and try to survive and rise in the ranks of   great houses of europe. Taken out of context some of the topics   discussed are quite jarring to the sensibilities. I assume if you are   listening to this you already know all of this. We are doing a   megacampaign spanning from the easiest start date in CK2 through to the   end then we are going to convert it for Europa Universalis 4 and play  it  through to the end of that game, basically a a victory lap before  CK3  comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

Just some very NSFW banter during a typical CK2 game. For those who don't know Crusader Kings 2 is a dynasty simulator where players create a family in medival europe and try to survive and rise in the ranks of great houses of europe. Taken out of context some of the topics discussed are quite jarring to the sensibilities. I assume if you are listening to this you already know all of this. We are doing a megacampaign spanning from the easiest start date in CK2 through to the end then we are going to convert it for Europa Universalis 4 and play it through to the end of that game, basically a a victory lap before CK3 comes out to celebrate which is in my opinion a perfect game.

It's been a minute since we've talked about Crusader Kings 2, so why bring it up now? Holy Fury - the latest major DLC - is one of the most significant changes to date, moving CK2 even closer toward RPG than grand strategy game. Is this the expansion that restores Fraser's interest in the game? Is this really the last expansion? What is the future of CK2? Why does T.J. go on and on and on and on about succession laws? Can you really be a duck? T.J. and Fraser answer all these questions and more.

Discuss this episode on our forums: https://pdxint.at/2PpwHoWIn this episode Ashkan Namousi, Producer at Paradox Interactive, talk about what a producer does and how working together with external game teams work. How do you avoid the game developer Stockholm Syndrome.The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Spotify: https://pdxint.at/2HFshtu Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Discuss this episode on our forums: https://pdxint.at/2PpwHoWIn this episode Blondie, Streaming Director at Paradox Interactive, hot off The Grandest LAN talk about streaming at Paradox and what this Grandest LAN is all about.The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Discuss this episode on our forums: https://pdxint.at/2PpwHoWIn this episode Paula Thelin, Event Manager at Paradox Interactive talks about what it takes to go to events with a games publisher, and the massive amounts of work it takes to create PDXCON.The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Discuss this episode on our forums: https://pdxint.at/2PpwHoWSusie McBeth, Senior Community Manager at Paradox Interactive, is invited onto the podcast to talk about what a community manager does and the challenges of the position. Social Media is only 10%.The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Recently, we announced and released Cities: Skylines for Nintendo Switch. In this episode of the Paradox Podcast we invite Jakob Munthe to discuss this announcement, release, and the Nintendo Direct in which we announced the port. The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Recorded at Gamescom, this episode of the Paradox Podcast brings on Sebastian Forsström, Product Marketing Manager for Stellaris: Console Edition to talkg about what it's like marketing a game on console and how it differs from PC. The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Recorded at Gamescom, this episode has a wide range of topics, including the announcement of Stellaris: Console Edition, the announcement of BattleTech: Flashpoint, trade shows, demographics, and more. The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development and Acquisitions) tries to shed a light on a part of the industry which is seldomly spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

In this episode of the Paradox Podcast Shams and Daniel discuss the recent acquisition of Harebrained Schemes and what this means for both companies. Also, acquisitions in general. The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development & Acquisitions) tries to shed a light on a part of the industry which is seldom spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Cette semaine, David nous parle de la série Evil Geniuses, Mathieu a joué au mod Game of Thrones de CK2 et Alex va nous parler du volume 1 de la série Thanos de Marvel comics.

Cette semaine, David nous parle de la série Evil Geniuses, Mathieu a joué au mod Game of Thrones de CK2 et Alex va nous parler du volume 1 de la série Thanos de Marvel comics.

Now on Spotify: https://pdxint.at/2HFshtu In this episode of the Paradox Podcast, Shams and Daniel discuss PDXCON 2018, and it's worth to Paradox as a company, at PDXCON 2018. The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development & Acquisitions) tries to shed a light on a part of the industry which is seldom spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Now on Spotify: https://pdxint.at/2HFshtu In this episode of the Paradox Podcast, Shams and Daniel discuss accessibility and difficulty in video games. Also there's a breakdown of what went down at GDC. The Paradox Podcast is a podcast about the business of video games, brought to you by Paradox Interactive. Daniel Goldberg (Marketing & Communications) and Shams Jorjani (Business Development & Acquisitions) tries to shed a light on a part of the industry which is seldom spoken of. Soundcloud: http://pdxint.at/2EYDl15 iTunes: http://pdxint.at/2BnBWBo Player.fm: http://pdxint.at/2EWTCnl Poddtoppen: http://pdxint.at/2CiqXGw Acast: http://pdxint.at/2ExBD9l Paradox on YouTube: --------------------------------- ParadoxInteractive - http://youtube.com/ParadoxInteractive Trailers, Feature Breakdowns, Dev Diaries, and more. ParadoxExtra - http://youtube.com/ParadoxExtra Gameplay of our video games, convention coverage, and other fun things from Paradox! Paradox Grand Strategy - http://youtube.com/ParadoxGrandStrategy Gameplay of our Grand Strategy Games. CK2, EU4, HOI4, Stellaris, and more?Support the show (http://paradoxplaza.com)

Gender blurring is a rejection of gender stereotypes and saluting an androgynous lifestyle. This trend has manifested itself in many ways: Calvin Klein's gender-free fragrance CK2, the declassification of toys by Disney and Amazon, and even Playboy's toned-down issue in March 2016. This trend will continue to impact the media, consumer ideals, and industries in the near future.        

TJ Hafer and Rowan Kaiser joing Rob Zacny to discuss the latest expansion to CK2, Horse Lords. Horse Lords allows the player to assume the role of the eastern hordes, galloping across Europe and the CK2 interface. Has CK2 finally been stretched to its limit?

Paradox returns to World War II with the upcoming Hearts of Iron IV and a recent press event found Rob, Rowan, Fraser, and Gamers with Jobs' Sean Sands playing a preview build of the simulation. The results? Mixed feelings. Amidst glimmers of hope for an improved HoI on the order of the successful EUIV and CK2, the panelists found a confusing interface, a lack of direction, and plenty of room for improvement before the final build.

Activity of the kinase CK2 promotes the generation of intracellular zinc waves by stimulating the zinc channel ZIP7.