Podcasts about cp26

Mich's Monday Mantra "Stop & Take In Everything Around You"===

Professor Prue Morgan is head of department at Monash University, Melbourne - Australia. She is a self professed yes person and expert in adults with cerebral palsy. We discuss her career journey, her passion for cerebral palsy and teaching philosophy.4:45 – Career path8:00 – Why cerebral palsy10:40 – Drop of in care for adults with CP11:50 – Filling the gaps in CP care13:00 – Keeping the passion14:20 – Neuroplastic potential vs compensate for success16:05 – key principles of working with CP18:30 – Team and importance of seating and positioning21:40 – Fatigue in CP23:30 – Barriers to participation in CP26:25 – Resources to assist CP knowledge for therapists30:15 – Prue's PhD34:00 – Transitioning into Academia35:30 – Teaching tips for successful student placements40:15 – Value of observation43:00 – Importance of feedback to grow and learn44:55 – Developing evaluation skills in students46:20 – Committee work and taking opportunitiesPrue's Monash Uni profilePrue's research output

Join Duc and Vince, as we chat with Jamie Gruber and Benoit Malige about building partnerships and leveraging human capital. Jamie and Benoit are Co-founders of the Multifamily and More group, a real estate investment club, helping its members achieve their financial and lifestyle goals through real estate investment. With their fantastic partnership, they were able to take the company from just being in a local market to expanding nationallly. The Advantages of Having a Co-founder Starting a business is a big responsibility and a lot of work. You can only do so much as a single founder. It can be discouraging for people who do not want to share their control of decision-making; however, there are so many advantages when you have a Co-founder. About Multifamily and More: We interview guests on virtual platforms, organize meetups in multiple cities and host events and mixers all aligned with our goal. We exist because we were sick of REIAs existing just to make money without giving anything back. We're committed to adding value and being a fresh face in the real estate meet up space. Go to MultifamilyAndMore.com to find out more! Outline of the episode: ·        [01:01]The key takeaways of the episode. ·        [01:46]How did Jamie and Benoit meet, and why did they decide to form a partnership? ·        [03:09]The story of Jamie and Benoit's journey of how they started to earn passive cash flow. ·        [05:23]Three ways you can build a passive income. Work hard on building credibility. ·        [07:36]Reasons why should you start building passive cash flow. ·        [09:52]Most recent deal they invested in and having the ability to overcome their struggles caused by the pandemic. ·        [14:39]What's their plan on the overall exit? ·        [18:14]Figure out what works best for you. Relationships in business are essential. ·        [19:50]Having good tenants depends on how well you screen them. Always maintain communication. ·        [24:21]What are Jamie and Benoit's biggest mistakes in the business? It's incredible how their opposite traits balance their strategic partnership so well. ·        [29:49]How did the Multifamily and More blow up from just being a local market?  ·        [36:20]What makes Multifamily and More different than the other investment clubs? ·        [38:52]The systems, technologies, and applications that they're using for their business. ·        [42:56]Being authentic, looking at small cuts that you can make on the expense side, and leveraging the scale where it's possible. ·        [45:57]Never Split the Difference, Interview with the Devil, Think and Grow Rich, How to Win Friends and Influence People. ·        [50:29]Where are Jamie and Benoit's favorite destinations?

7月，上海CP26漫展因为一件意想不到的事出圈了——一位身穿JK制服的女生因为拍照姿势露出了安全裤，而"意外"地被很多网友讨伐。 然而问题是，既然都穿了安全裤，露了不应该是正常的吗？为何相比起拍照的人，这位女生受到了更多责骂？为什么我们被要求穿内衣，但露出内衣又会成为一件不雅的事？所谓的穿衣自由的界限又在哪里？从这件事的舆论上看，"电子裹脚布"肉眼可见地裹挟着当代女性。0202年了，就别搞安全裤套娃了吧。 收听指北： 1.如何看待漫展JK女生事件中，其他女性的讨伐声？ 2.内衣悖论之：不能不穿，穿了又不能让人看出来穿了 3.被高度性化的女性身体 *节目观点仅代表主播本人，不代表看理想平台或赞助商。 本期「没理想好物推荐」：爱慕NYC芭尔无托背心式文胸。点击瞅瞅

7月，上海CP26漫展因为一件意想不到的事出圈了——一位身穿JK制服的女生因为拍照姿势露出了安全裤，而"意外"地被很多网友讨伐。 然而问题是，既然都穿了安全裤，露了不应该是正常的吗？为何相比起拍照的人，这位女生受到了更多责骂？为什么我们被要求穿内衣，但露出内衣又会成为一件不雅的事？所谓的穿衣自由的界限又在哪里？从这件事的舆论上看，"电子裹脚布"肉眼可见地裹挟着当代女性。0202年了，就别搞安全裤套娃了吧。 收听指北： 1.如何看待漫展JK女生事件中，其他女性的讨伐声？ 2.内衣悖论之：不能不穿，穿了又不能让人看出来穿了 3.被高度性化的女性身体 *节目观点仅代表主播本人，不代表看理想平台或赞助商。 本期「没理想好物推荐」：爱慕NYC芭尔无托背心式文胸。点击瞅瞅

Read the blog post:https://www.michrxconsulting.com/conversation-with-dr-christina-madison-part-2===

Talking Manpower sits down with Mr. Maurice Yaglom, Director, Manpower Policy, Programming and Allocation, Office of the Surgeon General. Mr. Yaglom speaks about his role, what Manpower and Force Managers are responsible for at OTSG and he looks back at his career.

Talking Manpower sits down with Col. Keith Rivers, Commandant, Army Force Management School. Col. Rivers discusses the importance of the school, its relevance to CP26 and what value FA50's and CP26's bring to the Army

Talking Manpower sits down with Col. Keith Rivers, Commandant, Army Force Management School. Col. Rivers discusses the importance of the school, its relevance to CP26 and what value FA50's and CP26's bring to the Army.

Talking Manpower continues its conversation with Mr. Ed Emden, Chief, Civilian Workforce Transformation. Mr. Emden discusses employee engagement, the Federal Employee Viewpoint Survey and some of the upcoming CWT initiatives.

Talking Manpower sits down with Mr. Ed Emden, Chief of Civilian Workforce Transformation. Mr. Emden discusses his time as a CP26 intern, his career path and explains all about CWT.

Talking Manpower sits down with Mr. Ed Emden, Chief of Civilian Workforce Transformation. Mr. Emden discusses his time as a CP26 intern, his career path and explains all about CWT.

Talking Manpower continues its conversation with Mr. Paul Hilton, Deputy Chief of Staff, G-8 at U.S. Army Training and Doctrine Command.

Talking Manpower speaks with Mr. Paul Hilton, Deputy Chief of Staff, G-8 at U.S. Army Training and Doctrine Command about his role and his experience as an FA50 Officer.

Talking Manpower sits down with Mr. Bill Kapaku, Senior CPI Specialist at Assistant Chief of Staff for Installation Management. During the discussion Mr. Kapaku speaks his role in ACSIM and speaks about Lean Six Sigma.

Talking Manpower finishes its conversation with Ms. Ellen Helmerson, who is the G-8 at U.S. Army Training and Doctrine Command.

Talking Manpower continues its conversation with Ms. Ellen Helmerson.

Talking Manpower sits down with Ms. Ellen Helmerson, U.S. Training and Doctrine Command Deputy Chief of Staff, G-8, to talk about her roles and responsibilities.

Talking Manpower continues its conversation with Mr. Jim Davis, Chief, Manpower and Management Division at U.S. Army Europe in Weisbaden, Germany. Mr. Davis speaks about interagency coordination and the benefits to working in Germany.

Talking Manpower sits down with Mr. Jim Davis, Chief, Manpower and Management Division at U.S. Army Europe in Weisbaden, Germany. Mr. Davis speaks about his role and some the complexities of working in Europe.

Talking Manpower continues its conversation with Ms. Alyssa Buxbaum, Management Analyst at the Resource Management Office, U.S. Army Garrison - Stuttgart

Talking Manpower continues its conversation with Ms. Susan Anthony, Director of Manpower and Force Analysis at U.S. Army Training and Doctrine Command.

Talking Manpower speaks with Ms. Alyssa Buxbaum, Management Analyst at the Resource Management Office, U.S. Army Garrison - Stuttgart

Talking Manpower speaks with Ms. Susan Anthony, Director of Manpower and Force Analysis at U.S. Army Training and Doctrine Command.

Ms. Tina Manns, Chief of Manpower and Force Management at U.S. Army Yuma Proving Ground, speaks about working an an overseas environment, why CP26's are resource managers and getting out of your comfort zone.

Ms. Tina Manns, Chief of Manpower and Force Management at U.S. Army Yuma Proving Ground, speaks about working an an overseas environment, why CP26's are resource managers and getting out of your comfort zone.

Talking Manpower sits down with Ms. Karen Taylor, Chief of the Manpower and NATO Agreements Division, U.S. European Command

Talking Manpower continues its conversation with Mr. Jay Aronowitz about his career and his thoughts on leadership.

Talking Manpower continues its conversation with Mr. Jay Aronowitz about his career and his thoughts on leadership.

Talking Manpower speaks with Mr. Jay Aronowitz about his career and his thoughts on leadership.

Talking Manpower speaks to Lt. Col. Ed Serrano, Chief Functional Area 50 Personnel Development Office about FA50's role in Army Manpower.

Talking Manpower speaks with Col. Keith Rivers, Deputy Commander, U.S. Army Force Management Support Agency about the role his organization plays in the Army Manpower field.

Talking Manpower continues its conversation with Dr. Robert Steinrauf, Chief of Plans and Resources Directorate, Deputy Chief of Staff G-1 about leadership.

Talking Manpower speaks with Mr. Robert Lebron from the Army Force Management School about the importance of professional education.

Talking Manpower speaks with Dr. Robert Steinrauf, Chief of Plans and Resources Directorate, Deputy Chief of Staff G-1 about leadership.

Talking Manpower speaks with Ms. Frances Rivera who is a Manpower Analyst assigned to the Office of the Assistant Chief of Staff for Installation Management about her career path and having a 'Ah-ha' Moment

Talking Manpower speaks with JB Brown about how to develop Manpower skills and how to prepare for an opportunity to work overseas.

CP26 Proponency Chief Ms. Beryl Hancock speaks to AFN Stuttgart about the Manpower and Force Management Course hosted in Stuttgart, Germany, Sept. 12, 2014.

CP26 Proponency Chief Ms. Beryl Hancock speaks to AFN Stuttgart about the Manpower and Force Management Course hosted in Stuttgart, Germany, Sept. 12, 2014.

Etwa 30% aller Gene codieren für Membranproteine (MP). Trotz ihrer hohen Relevanz, speziell im medizinischen Bereich, stellt die Analyse von MP aufgrund ihrer physikalisch-chemischen Eigenschaften ein häufiges Problem in der Proteinbiochemie dar. Diese Arbeit soll eine Einsicht in die Problematik geben sowie Lösungsansätze aufzeigen, um den Umgang mit diesen Polypeptiden zu vereinfachen. Ein geeignetes Modellsystem zum Studium der Eigenschaften membranintegraler Proteine und Peptide sowie zur Verbesserung der bestehenden Analysemethoden stellte die Thylakoidmembran der Plastide dar. Um das funktionelle Proteom der Thylakoidmembran zu definieren, wurden die Proteinkomplexe der Thylakoidmembran von Gerste (Hordeum vulgare) über hochauflösende 2D-Blue Native /SDS-Polyacrylamidgelelektrophorese (PAGE) getrennt. Das Gelsystem erlaubte die Isolation der photosynthetisch aktiven Proteinkomplexe PSI/LHCI, PSII, LHCII, Cytochrom b6/f und ATPase in unterschiedlichen Assemblierungszuständen. Im Fokus der Untersuchungen stand die Charakterisierung der isolierten Subkomplexe von PSII. Die Identifikation der Komplexuntereinheiten erfolgte nach enzymatischem In-Gel Verdau und massenspektrometrischer Analyse der entstandenen Peptide (offline nanoESI-MSMS). MP > 10 kDa wurden ausschließlich über Peptide aus den löslichen Abschnitten identifiziert. Die Analyse der niedermolekularen Untereinheiten (< 10 kDa) wurde auf Ebene des Gesamtproteins nach Extraktion aus den Komplexbanden der BN-PAGE realisiert. Dabei konnten dem mono- und dimeren PSII-Subkomplex folgende niedermolekularen UEn zugeordnet werden: PsbE, PsbF, PsbI, PsbK, PsbL, PsbM, PsbTc und PsbX. Da kein Unterschied in der Zusammensetzung des mono- und dimeren PSII-Subkomplexes existierte, konnte eine Beteiligung einer der niedermolekularen UEn an der Ausbildung des dimeren PSII-Subkomplexes im Rahmen der Assemblierung nicht bestätigt werden. Die Lichtsammelproteine (LHCP) des LHCII wurden nach 2D BN/SDS-PAGE auf Ebene der Superkomplexe oder abgetrennt als Mono- und Trimerer LHCII-Subkomplex identifiziert, wobei das Trimer durch das Fehlen der minoren LHCP (CP29, CP26 und CP24) charakterisiert war. Die für Membranproteine der Thylakoide ungewöhnlich hydrophilen LHCP erhielten die benötigte Hydrophobizität zur Durchspannung der Membran über die Bindung von Pigmenten (Chlorophyll). Eine eindeutige Unterscheidung der Genprodukte von Lhcb1-3 war trotz extremer Sequenzhomologie über die Detektion eines charakteristischen Peptids im N-terminalen Bereich der maturen Sequenz möglich. In Gerste wurde somit jeweils eine Form von Lhcb2 und 3, sowie sechs Isoformen von Lhcb1 identifiziert. Um den In-Gel Verdau von Proteinen nach elektrophoretischer Trennung zu vereinfachen und zu standardisieren, wurde ein Reaktionsgefäß (OMX-S®) aus Polypropylen entwickelt. Im Zuge der Anpassung des konventionellen Protokolls zum In-Gel Verdau von Proteinen für OMX-S® wurde ein optimiertes Verdauprotokoll entwickelt, das ohne die Reaktionsschritte Entfärbung, Reduktion & Alkylierung der AS Cystein sowie eine multiple Extraktion zur Anreicherung der entstandenen Peptide auskommt. Die Erhöhung der Reaktionstemperatur auf 50°C und die Verkürzung der Diffusionsstrecke für die Protease erhöhten zudem die Effizienz des Verdaus und führten zu einer Reduktion der gesamten Prozesszeit von 6-24 h auf 1 h. Welche Auswirkung die Auslassung einzelner Reaktionsschritte auf die Peptidausbeute hatte, wurde nach differentieller Isotopenmarkierung der generierten Peptide mittels massenspektrometrischer Analyse quantifiziert. Da jeder Prozessierungsschritt eine potentielle Quelle für Verluste darstellte, waren die Peptidausbeuten im Vergleich zum konventionellen In-Gel Verdau äquivalent oder sogar besser. Unabhängig vom verwendeten Verfahren, fehlten die membranintegralen Peptide in den Spektren. Folglich wurde die Detektierbarkeit und Signalintensität von tryptischen Peptiden in Abhängigkeit von verschiedenen Faktoren untersucht. Dabei ergab sich eine direkte Korrelation zwischen der Proteinmenge einer Bande und der Anzahl, der nach Verdau detektierten Peptide. Die Untersuchungen an Peptiden aus löslichen und membranintegralen Proteinen ergaben, dass die Hauptursache für das Fehlen letzterer, nicht auf den Einfluss bestimmter AS auf die Ionisierbarkeit, die Sequenzlänge und/oder die Hydrophobizität zurückzuführen war. Entscheidend für die Abwesenheit der membranintegralen Peptide war vielmehr die schlechte Zugänglichkeit der Schnittstellen für die Protease, aufgrund unzureichender Denaturierung der Sekundärstruktur bzw. der Aggregation hydrophober Abschnitte im Rahmen der Probenaufarbeitung.

Plastid chromosomes from the variety of plant species contain several conserved open reading frames of unknown function, which most probably represent functional genes. The primary aim of this thesis was the analysis of the role of two such ORFs, designated ycfs or hypothetical chloroplast reading frames, namely ycf9 (ORF62) and ycf10 (ORF229, cemA). Both were analyzed in Nicotiana tabacum (tobacco) via their inactivation using biolistic plastid transformation. A new experimental protocol, based on pulsed-field gel electrophoresis (PFGE), was established to reliably assess the homoplastomic state of transformed plants. 1. Functional analysis of the ycf9 gene product: The inactivation of ycf9 in N. tabacum as well as in Chlamydomonas reinhardtii yielded a homoplastomic mutant phenotype after several rounds of regeneration under selective pressure. The mutant plants grew photoautotrophically, but displayed two clear phenotypes, a light-sensitive one, increasing with the light intensity, and a dwarf phenotype under low-light combined with temperatures below 20°C. The ycf9 gene product was exclusively located in PSII core complexes. This localization was based on the isolation of protein complexes released from thylakoids by controlled, partial lysis, followed by sucrose density gradient centrifugation or 2D gel electrophoresis. This finding revised data of the literature. Biochemical analysis indicated an involvement of the protein in the interaction of the light harvesting antenna II complex (LHCII) with PSII cores. In particular, PSII-LHCII supercomplexes could no longer be isolated from transplastomic tobacco plants. Furthermore, the minor chlorophyll a/b-binding proteins CP26, and to a lesser extent CP29, were substantially reduced under most growth conditions analyzed, in both, tobacco and photoautotrophically grown Chlamydomonas mutants (Swiatek et al. 2001). The gene was therefore renamed psbZ. The ∆psbZ-related alterations in the supramolecular organization of PSII complexes were accompanied by considerable modification in (i) the phosphorylation pattern of PSII subunits, (ii) the rate of deepoxydation of xanthophylls, and (iii) the kinetics and amplitude of non-photochemical quenching. The proposed position of PsbZ in close proximity to CP43 enables the protein to interact with PSII cores to elicit an adaptation process in response to excess light excitation. The molecular mechanism underlying this energy dissipation process remains to be investigated. 2. Functional analysis of the ycf10 gene product: Biolistic plastid transformation was also used to inactivate the ycf10 reading frame in tobacco. After several rounds of regeneration under selective pressure, homoplastomic plants were obtained. Northern analysis uncovered co-transcription of ycf10 within the psaI-ycf4-ycf10-petA gene cluster, with at least two promotor regions upstream of the psaI gene. The mutant plants grew photoautotrophically and developed dark green leaves with numerous pale green to white regions, the latter devoid of photosynthetic activity. The loss of ycf10 did not affect photosynthetic activity, as indicated by unaltered chlorophyll fluorescence. The tobacco ycf10 gene product was localized in the chloroplast inner envelope membrane. Neither protein composition of stroma or thylakoid fractions, nor the stability of the photosynthetic protein complexes were affected in the mutant plants. In contrast, CO2- dependent oxygen evolution was strongly reduced, with a maximum rate of Ci-dependent photosynthesis being approximately 50% lower than in wild-type plants. Two explanations can account for the observed phenomenon: (i) de-regulation of carbonconcentrating mechanisms in transformed cells, or (ii) an indirect effect on CO2-uptake in ∆ycf10 plants. 3. Pulsed-field gel electrophoresis is an ideal tool to verify the homoplastomic state of transformed plants: To enhance the sensitivity of detection of heteroplastomic states, and to distinguish between plastome-located wild-type segments in transplastomic material and promiscuous DNA, a new approach was developed. Customary Southern and PCR techniques are not sensitive enough or not discriminating the latter alternatives, respectively. Pulsed-field gel electrophoresis allows to isolate virtually contamination-free plastid DNA. Plastid DNA isolated this way lacked traces of nuclear and mitochondrial DNA at a detection level of 50 DNA molecules. This excludes that gene-specific PCR amplification products originate from promiscuous nuclear or mitochondrial gene copies. Therefore, PFGE appears to be an ideal tool to investigate the homoplastomic state of transformed plants, especially when combined with radiolabeled probes and Southern techniques.