Podcasts about diploid

Potato DNA is unique from human and most animal DNA, becuase they're of tetraploid origin, meaning they have four copies of each chromosome in their DNA – two from each plant parent, as opposed to one. Because of the vast number of tetraploid varieties, potato varieties become closely related to one another and more genetically similar. Decreased genetic diversity leads to varieties being more vulnerable to different diseases, and less adapted to climate change.One step scientists are taking to increase genetic diversity is working with potato relatives that are tetraploid or diploid. Wild diploid potatoes have two copies of each chromosome in their DNA, making their genetics easy to work with. Diploid potatoes are smaller, but hold true breeding potential because of their unexplored genetic traits.AAFC research scientist Bourlaye Fofana discusses the work he and his team are doing with breeding diploid potatoes – from the desirable traits of the 50 most promising diploid clones, especially as it relates to drought resistance, to why taste-testers do indeed play an important role in in the scientific process.

BUFFALO, NY- October 25, 2023 – A new research paper was published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 15, Issue 19, entitled, “Live while the DNA lasts. The role of autophagy in DNA loss and survival of diploid yeast cells during chronological aging.” Aging is inevitable and affects all cell types. Thus, yeast cells are often used as a model in aging studies. There are two approaches to studying aging in yeast: replicative aging, which describes the proliferative potential of cells, and chronological aging, which is used for studying post-mitotic cells. In this new study, while analyzing the chronological lifespan (CLS) of diploid Saccharomyces cerevisiae cells, researchers Tuguldur Enkhbaatar, Marek Skoneczny, Karolina Stępień, Mateusz Mołoń, and Adrianna Skoneczna from the Polish Academy of Sciences and Rzeszów University discovered a remarkable phenomenon: ploidy reduction during aging progression. “To uncover the mechanism behind this unusual process we used yeast strains undergoing a CLS assay, looking for various aging parameters.” Cell mortality, regrowth ability, autophagy induction and cellular DNA content measurements indicated that during the CLS assay, dying cells lost their DNA, and only diploids survived. The researchers demonstrated that autophagy was responsible for the gradual loss of DNA. The nucleophagy marker activation at the start of the CLS experiment correlated with the significant drop in cell viability. The activation of piecemeal microautophagy of nucleus (PMN) markers appeared to accompany the chronological aging process until the end. “Our findings emphasize the significance of maintaining at least one intact copy of the genome for the survival of post-mitotic diploid cells.” During chronological aging, cellular components, including DNA, are exposed to increasing stress, leading to DNA damage and fragmentation in aging cells. The researchers propose that PMN-dependent clearance of damaged DNA from the nucleus helps prevent genome rearrangements. However, as long as one copy of the genome can be rebuilt, cells can still survive. “The observations we made in aging research using yeast as the eukaryotic cell model may help to understand the mechanisms that prevent aneuploidy during aging or cancerogenesis in cells where chromothripsis has occurred.” DOI - https://doi.org/10.18632/aging.205102 Corresponding author - Adrianna Skoneczna - ada@ibb.waw.pl Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205102 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, genome instability, lifespan, autophagy, double-strand breaks About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at https://www.Aging-US.com​​ and connect with us: SoundCloud - https://soundcloud.com/Aging-Us Facebook - https://www.facebook.com/AgingUS/ X - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

Breaking down Too Bird's Melbourne 2 with the whole Too Birds crew, Mr. Society, Realname & Teether. Dan & Maia dig into one of the best Australian releases of the year, what exactly inspires the group's experimental hip-hop sound, as well as what ever happened to Melbourne 1? TRACKS PLAYED: ‘688aud' ‘Monstera Heaven' ‘Gambit' ‘Loudest' ‘Showers' ‘God's Pharmacy' ‘7000000000 Chumps' ‘I'm The Peak' ‘Easy 2 Love feat. Slug Christ' ‘I Live Here feat. Diploid' ‘Snake City (Take me There…)'. See omnystudio.com/listener for privacy information.

Intro: Serene Ailment Incantation Praxis from cold_storage   Mindy Meng Wang An Improvisation Through Time and Space 穿越时光的即兴  (Music in Exile, 2020) Forbidden City, A Cold Moon 故宫 . 冷月Clown's New Trick 小丑的新把戏Thoughts in the Rain 落雨思 Dimensions of a Strange Land 异境 Australian Art Orchestra, Reuben Lewis, Tariro Mavondo, Peter Knight Closed Beginnings (2021)We Too, Roar Firefly Jars Creases of Gods Sweet Sticky Revolution Diploid I AM YOURS. AND I AM HERE AGAIN (Heavy Machinery, flash fwd 2021)

After yesterdays broadcast on DI FM we present you the 86th episode of the Spring Tube podcast. During 2 hours here for you another selection of some of the brightest this month's stuff from Spring Tube and other labels. Your hosts are SlanG and Technodreamer. For our August 2021 episode we have a new music from Michael & Levan and Stiven Rivic feat. Rick Pier O'Neil, Ewan Rill, Audioglider, Monojoke, Diploid, and many others. In a last 30 minutes of this month's podcast we have a special review mix of Da Funk's 'Personal Space' compilation album just released on our label. Have a nice listening *available for download Tracklist: PART 1 – SlanG 01. Banaati & Aryan Saleh - Wide Awake [Songspire] 02. Diploid - Way Too Long [ICONYC] 03. Jonny Vee - Momentum [Spring Tube Limited] SPRLTD150 04. Krav - No Sleep [Batavia] 05. Supernova - Lovely Summer Dreams [Blackboard] 06. Apriori - Can't Stop Loving You (Ewan Rill 'Poolside' Remix) [RSNNC] 07. Does It Matter feat. Lewyn - Breathe Again [Songspire] 08. Storgards & Laurence Lane feat. Daniel Gidlund - Ejen [Where The Heart Is] 09. Audioglider - A Month Of Sundays [Manual] 10. VASSY feat. Sultan + Shepard - Somebody New (Sebastian Davidson Remix) [Armada Chill] PART 2 – Technodreamer 01. Kei How - Gessyoku [Spring Tube Limited] SPRLTD151 02. Monojoke - Sacred Leafs [Nature Rec.] 03. Kabi & K Loveski - Whisper From The Moon [Juicebox] 04. EMPHI - Stockholm Syndrome [Deepwibe Underground] 05. KYOTTO - Sweet Escape (Alan Cerra Remix) [Dopamine White] 06. A-Mase - Introvertum [Amase Digital] 07. Ruben Karapetyan - Never Again [Movement] 08. Michael & Levan and Stiven Rivic, Rick Pier O'Neil - Monsoon [Univack] 09. Jorge Viana - Kites In The Ground [Transensations] PART 3 – Da Funk 'Personal Space' review mix 01. Da Funk - Choke [Spring Tube / Acryl] SPR306PS19 / ACRM084 02. Subsky - Strawberry Fields (Da Funk's Double Dragon Dub) [Spring Tube] SPR306PS19 03. Da Funk - Ruffle Up [Spring Tube] SPR306PS19 04. Phrakture feat. Maria Carmela - Curve Sound (Da Funk's Recovery Dub) [Spring Tube] SPR306PS19 05. Da Funk - You Got Me [Spring Tube] SPR306PS19

Today on the Mushroom Hour Podcast we are joined by Dr. Michael Perlin, Professor in Biology at the University of Louisville. Dr. Perlin's interests drew him to study host/pathogen interactions at the University of Chicago and his PhD work centered on the evolution of bacterial resistance to certain classes of antibiotics/antimicrobials. As he was near to completing his degree, a friend of his stopped by the lab and introduced him to a different microbial system: a fungal pathogen that infects flowering species in the Carnation family and replaces the pollen of the flower with fungal spores. From that point he was hooked. Over the years he has continued to work on bacterial resistance, but in the last 10 years, he has shifted the focus of his lab exclusively to that on fungal plant pathogens, with projects encompassing three different types of pathogens on different hosts and the use of the yeast, Saccharomyces cerevisiae, as a model tool in the exploration of some questions more easily investigated in this well-developed system. Dr. Perlin has roughly 70 peer-reviewed scientific articles in reputable journals, including Nature; mBio; BMC Genomics; Genes, Genomes, Genetics (GGG); Fungal Genetics and Biology; Journal of Bacteriology; Eukaryotic Cell; and Molecular Microbiology. As a member of a number of scientific organizations and a prolific teacher and mentor, Dr. Perlin has a far-reaching influence on his field and has directly played a role in the careers of many PhD students. I'm excited to learn more about how fungal phytopathogens that have a massive impact on human culture.   TOPICS COVERED:   Journey into the Life Sciences   Influence of Dr. Stephen Lerner   Antibiotic Era & Understanding Microbial Evolution in Preventing Antibiotic ResistanceFungal Phytopathogen Interactions   Emerging Disease from Fungal Plant Pathogens as Primary Threat to Modern Human Society   Physiology and Life Cycle of Smut Fungi  Systems Studied Over Three Decades of Research at University of Louisville  Extrapolating Insights from Fungal Pathogens to Other Systems  Dimorphic Switch from Benign Yeast Form to Infectious Filamentous Form  Signaling Pathways in Cells Triggering Dimorphic Transformation  Precautionary Principle in Science   Protein “Effectors” Produced by Pathogens that Manipulate Hosts   Perlin Lab and Collaborative Science   Studying Mitochondria Inheritance in Model Fungal Systems   EPISODE RESOURCES:   Dr. Perlin Website: https://louisville.edu/biology/faculty/perlin   Stephen Lerner (Inspiration): https://photoarchive.lib.uchicago.edu/db.xqy?one=apf1-10357.xml   Microbotryum violaceum (fungus): https://en.wikipedia.org/wiki/Microbotryum_violaceum   Ustilago maydis (fungus): https://en.wikipedia.org/wiki/Corn_smut   Coccidia (pathogen): https://www.sciencedirect.com/topics/medicine-and-dentistry/coccidia   Ophiocordyceps unilateralis (fungus): https://en.wikipedia.org/wiki/Ophiocordyceps_unilateralis   

In part 3  of our "Triploid Trilogy", Dr. Brett Hartis, Lead Scientist at Duke Energy brings his expertise and experience to our Grass Carp conversation.  Dr. Hartis  has to manage these very large extensive Reservoirs for Duke Power and he shares how he  has worked with Triploid Sterile Grass Carp.Dr. Hartis shares his knowledge and experience to answer these questions and more:What is the difference between Diploid and Triploid Grass Carp?What does a grass carp eat?Why and when do you need Grass Carp  in your pond or lake?You don't want to miss his Grass Carp stocking strategies, his results, and how he got these results.  Come Sit with us,  on Sitting Dockside!Have questions or want to tell us your thoughts on this subject? Join our Facebook Community:  https://www.facebook.com/groups/PWNRA/  Want help with in your area with your pond or lake?  Check out our trusted industry sponsors at our P.W.N.R.A Sponsor Directory .Support the show (http://www.pwnra.org)

In Folge 2 beschäftigen wird uns mit dem aktiven und passiven Stofftransport und klären dabei die Begriffe Diffusion, Osmose, Zellteilung und Reduktionsteilung. Ein Begleitvideo findest du unter: https://youtu.be/EIFlBcacpnc Fragen, die wir in dieser Folge klären werden sein: Wie kommen Stoffe in die Zelle und aus der Zelle heraus? Und warum muss Stofftransport überhaupt sein? Was ist der Unterschied zwischen Filtration, Diffusion und Osmose? Wie vermehrt sich eine Zelle und worin unterscheidet sich die Zellteilung einer "normalen" Zelle von der einer Keimzelle? Viel Spaß beim Lernen! UPDATE: David hat mich auf einen Fehler hingewiesen, vielen Dank dafür, David! In Minute 2:01 spreche ich von der Synthese von Membranproteinen im glatten endoplasmatischen Retikulum. Die werden aber im rauen endoplasmatischen Retikulum produziert. Also, sorry für den Versprecher. Zum Glück brauchen wir das aber nicht so tief wissen :-) Hier kannst du mich und den Podcast unterstützen: https://steadyhq.com/wissensreise

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.10.27.356907v1?rss=1 Authors: Heller, D., Vingron, M. Abstract: Motivation: With the availability of new sequencing technologies, the generation of haplotype-resolved genome assemblies up to chromosome scale has become feasible. These assemblies capture the complete genetic information of both parental haplotypes, increase structural variant (SV) calling sensitivity and enable direct genotyping and phasing of SVs. Yet, existing SV callers are designed for haploid genome assemblies only, do not support genotyping or detect only a limited set of SV classes. Results: We introduce our method SVIM-asm for the detection and genotyping of six common classes of SVs from haploid and diploid genome assemblies. Compared against the only other existing SV caller for diploid assemblies, DipCall, SVIM-asm detects more SV classes and reached higher F1 scores on two recently published assemblies of the HG002 individual. Availability and Implementation: SVIM-asm has been implemented in Python and can be easily installed via bioconda. Its source code is available at github.com/eldariont/svim-asm. Contact: vingron@molgen.mpg.de Supplementary information: Supplementary data are available online. Copy rights belong to original authors. Visit the link for more info

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.08.29.273722v1?rss=1 Authors: Ye, T., Ma, W. Abstract: The recently developed Hi-C technique has been widely applied to map genome-wide chromatin interactions. However, current methods for analyzing diploid Hi-C data cannot fully distinguish between homologous chromosomes. Consequently, the existing diploid Hi-C analyses are based on sparse and inaccurate allele-specific contact matrices, which might lead to inaccurate modeling of diploid genome architecture. Here, we present ASHIC, a hierarchical Bayesian framework to model allele-specific chromatin organizations in diploid genomes. We developed two models under this Bayesian framework: the Poisson-multinomial (ASHIC-PM) model and the zero-inflated Poisson-multinomial (ASHIC-ZIPM) model. The proposed ASHIC methods impute allele-specific contact maps from diploid Hi-C data and simultaneously infer allelic 3D structures. Through simulation studies, we demonstrated that our methods outperformed existing approaches, especially under low coverage and low SNP density conditions. Additionally, we applied ASHIC-ZIPM to a published diploid mouse Hi-C data and studied the active/inactive X chromosomes and the H19/Igf2 imprinting region. In both cases, our method produced fine-resolution diploid chromatin maps and 3D structures, and provided insights into the allelic chromatin organizations and functions. To summarize, our work provides a statistically rigorous framework for investigating fine-scale allele-specific chromatin conformations. Copy rights belong to original authors. Visit the link for more info

Meanjin/Brisbane post-punk 4-piece CNT EVN burst onto the local music scene in 2018. They put out a self-titled and self-released digital demo in September of that same year and recently released their EP “Tryna” on Team Glasses Records in early May of 2020. Saturday 25th July will see Cnt Evn play at The Zoo as part of the ‘Anti-social Series’ along with Brisbane punk rock band Total Pace. This episode features an interview by my good friend Chris Preindl with Cnt Evn guitarist Anthony which originally aired on his radio show Swiss Air on 4zzz last month around the release of TRYNA. After the interview I have included a live recording that I captured at Fat Louie’s in Brisbane at their show with Diploid, Lizzard Wizzard, Common Enemy, Hexmere and Oratory on 1st of February 2020. Follow CNT EVN: https://www.facebook.com/cntevnband https://www.instagram.com/cntevnband https://cntevn.bandcamp.com/ Cnt Evn’s new EP ‘Tryna’ is available here: https://teamglasses.bandcamp.com/album/tryna Tickets for Total Pace & Cnt Evn on 25 July at The Zoo, Brisbane https://zoo.oztix.com.au/outlet/event/0e57c539-0df1-4282-af6d-385cb15fae0e?fbclid=IwAR2GxgMSY6usMA2kufsl9dq1SPKIVGlN1wOHQDQzc3syc0OqcCJgcLIqPDU Swiss Air on 4zzz (every Monday 1pm – 3pm on 102.1FM in Brisbane) Artwork in cover image by Rae Hatch Team Glasses Podcast is created on the traditional lands of the Turrbal and Jagera people. We pay respects to their elders past present and emerging. Sovereignty was never ceded.

Holy cow, it's the final episode of 2019! After watching their beautiful EP launch, I caught up with Sean & Tam of Obscura Hail to chat about Fenn Is Cool, Woodes, Diploid, Georgia Maq, LVIV & Eternal Crush.Thanks for listening in 2019 friends! We'll be sharing our 2019 year wrap across two parts (as usual) in early January.Intro music is 'Crash Pad' by AtaleinOutro music is 'Swear Jar' by Obscura Hail For information regarding your data privacy, visit Acast.com/privacy See acast.com/privacy for privacy and opt-out information.

  There are a lot of weird Tick stories in the news lately.  From stories of exsanguinated cows in North Carolina, to books about government conspiracy and bioweapons testing of ticks as delivery vehicles for disease, it seems everybody's trying to latch onto tick and tick-borne disease stories.  Acarologist Dr. Danielle Tufts, visits with us to help debunk some of the outright myths about ticks, to tell us more about how these diseases are transferred, and to get a better grip on how frightened we should be, and what we can do to protect ourselves from the millions of tiny vampires waiting out there. Photos of tick lifecycle and academic articles on relevant tick species are attached to the show notes. Some terms from the show you might not be familiar with: Serology - the scientific study or diagnostic examination of blood serum, especially with regard to the response of the immune system to pathogens or introduced substances. We discuss three types of chromosomal polyploidy: Diploid - containing two complete sets of chromosomes Triploid - containing three complete sets of chromosomes Aneuploid - contains an abnormal number of chromosomes

"Melbourne’s Diploid return with ‘Everything Went Red’, an all-out assault of sludge-ridden punk, doom-laden screamo, and blackened grind. The new record shows just how much the band’s relentless touring, writing and rehearsing has paid off. On ‘Everything Went Red’, influences from noise acts like The Body and Merzbow can be heard alongside a shift towards slower, grinding tempos of sludge and doom. When mixed with their base of powerviolence, screamo, punk and grind, the effect is immense." Ben talked to Reece about the new album, Everything Went Red, his approach to noise and interest in serial killers. Aired 26th October 2017. Tune in to SYN's Punk and Metal Night, Moshpit, on 8pm-11pm Thursdays, on SYN 90.7 FM & Digital, and online at www.syn.org.au. Facebook: https://www.facebook.com/Moshpitonsyn/ Follow us on Twitter and Instagram: @MoshpitSYN Hashtag: #MoshpitonSYN Website: www.SYN.org.au/Moshpit Subscribe to YouTube Channel: https://www.youtube.com/user/moshpitonsyn?sub_confirmation=1 Omny Podcast: http://omnyapp.com/shows/moshpit Subscribe on iTunes: https://itunes.apple.com/au/podcast/moshpit-backstage-podcast/id1103834864?ls=1

EPISODE 10 (Part 1) – SIBERIAN HELL SOUNDS I went over to Dan Young’s house after work late on a Saturday night to talk about his band, the Brisbane-based blackened hardcore of SIBERIAN HELL SOUNDS and their new release ‘SVENGALI”. LINKS: https://www.facebook.com/Siberianhellsounds https://siberianhellsounds.bandcamp.com/ MUSIC: ‘A Cult Will Rise’ by Siberian Hell Sounds (from ‘split with NØNE’) ‘Tension’ by Siberian Hell Sounds (from ‘S/T EP’) ‘Finding a Way’ by NØNE (from ‘split with Siberian Hell Sounds’) ‘⍲’ by Siberian Hell Sounds (from ‘⍲⍕⍎⍱’) ‘Ishtar’ by Siberian Hell Sounds (from ‘split with Travels’) ‘Charges of Torture’ by Siberian Hell Sounds (from ‘The PCP EP’) ‘Ripen’ by Siberian Hell Sounds (from ‘Svengali’) ‘Seed’ by Siberian Hell Sounds (from ‘Svengali’) ‘Ingest’ by Siberian Hell Sounds (from ‘The PCP EP’) ‘Odium’ by Hexis (from ‘split with This Gift is a Curse’) ‘Change’ by Blight Worms (from ‘I’) ‘Coping’ by Diploid (from ‘split with Lytta’) ‘Collapse and Still Lost’ by Gvrrls (from ‘Protect Us From the Ones We Love’) ‘Tomo’ by BØG (from ‘split with ITHAQUA’) ‘Preach Chaos’ by Shadowölf (from ‘Crust Wizard’) ‘Lilin’ by Melchior (from ‘It Means Nothing to Me’) ‘Franky und Kator’ by Iron Worzel (from www.reverbnation.com/ironworzel3/songs ) ‘Sixteen’ by Whitehorse (from ‘Raised into Darkness’) ‘Human Capital’ by Ashmouth (from ‘S/T’) ‘Maraud’ by Sundr (from ‘Loss’) ‘Cursed’ by Drowning Horse (from ‘Sheltering Sky’) ‘Issues’ by SHACKLΣS (from ‘Forces to Regress’) ‘Boot Straps’ by Descent (from ‘S/T’ demo) ‘Cops Get Pissed’ by Shitgrinder (from ‘split with Coffin Birth’) ‘Fast World’ by Perpetrator (from ‘Cursed’) ‘Youth Undone’ by Endless (from ‘Three Song Tape’) ‘Conditions’ by Deafcult (from ‘Ten Years (Thank You)’ comp) ‘‘Svengali’ by Siberian Hell Sounds (from ‘Svengali’)

EPISODE 9 - DIPLOID On their recent trip up to Queensland to play shows in Brisbane & Ipswich, I spoke with Mariam Benjamaa, Reece Prain & Scott Mclatchie of Melbourne-based blackened hardcore/noise band DIPLOID at Kangaroo Point Cliffs, Brisbane. NB: The second part of this podcast had some technical difficulties with my recording device while we were driving from the Ipswich back to Dan from Siberian Hell Sounds’ place in Brisbane, where Maz, Reece and Scott were staying. I decided to include it as we had an interesting discussion about the themes and lyrics on ‘Is God Up There?’ and I didn’t want it to not be included. It’s a rough listen but worth I if you can stick with it. LINKS: https://www.facebook.com/diploidmelb https://diploid.bandcamp.com/ https://www.facebook.com/artascatharsis https://www.facebook.com/LifeLairRegretRecords MUSIC: “Doomed From The Start” by Diploid (from ‘Is God Up There?’) “Corrections” by Diploid (from ‘Is God Up There?’) “Blood & Ash” by Rebirth (from ‘Crucible’) “Wrath of the Immaculate” by XLAIRX ( from ‘Wrath of the Immaculate’) “Your Own Good” by Mt. Zero (from ‘split w/ Flare Up’) “Until I’m More” by Diploid (from ‘Is God Up There?’) “Barrels” by Diploid (from ‘Is God Up There?’) “Spoils of Ripe” by Blind Girls (from ‘He Who Swallowed the Bloom’) “Wuornos” by Diploid (from ‘Is God Up There?’) “In the Toybox” by Diploid (from ‘Is God Up There?’) “Back Alley Abortion” by Diploid (from ‘Human’) “I Don't Remember Your Face Anymore” by Diploid (from ‘Is God Up There?’) “People Like Haley” by Diploid (from ‘Is God Up There?’)

I chat to Rose Wadsworth & James Rolph from the socially conscious hardcore punk band MASOCHIST (Northern Rivers, NSW) after their set at the Indooroopilly Hotel in Brisbane with Rebirth, Unravel, Hurricane Death and Endless. LINKS: https://www.facebook.com/mschist http://masochistpunk.bandcamp.com MUSIC: “Homewrecker” by Masochist (from ‘Malevolent’) “A Missed Chance For A Meaningful Abortion” by Dangers (from ‘Anger’) “Hostile” by Masochist (from ‘Malevolent’) “Just Ace” by Grinspoon (from ‘A Guide to Better Living’) “Ready to Fight” by Negative Approach (from ‘s/t’) “You Make Us Sick” by Outbreak (from ‘You Make Us Sick’) “Rage” by Angel Du$t (from ‘Xtra Raw’) “Disease” by Gouge Away (from ‘Disease’) “Coward” by Krimewatch (from ‘Demo’) “You Don’t Tell Me” by Super Unison (from ‘Auto’) “Walk Away” by Super Unison (from ‘S/T’ 7”) “Don’t Start” by Punch (from ‘S/T’ LP) “Lilin” by Melchior (from ‘It Means Nothing to Me’) “Thronebreaker” by Dispossessed (from ‘Insurgency’) “Nothing For You” by Masochist (from ‘Malevolent’) “Six Pack” by Black Flag (from ‘Damaged’) “Corrections” by Diploid (from ‘Is God Up There?’) “Not Welcome” by Masochist (from ‘Malevolent’) “Shame” by Masochist (from ‘Malevolent’)

I chat to Drew Eicke about his label Naked Noise Records in the Brisbane City Botanic Gardens, Queensland. NAKED NOISE RECORDS https://www.facebook.com/Naked-Noise-Records-642297932518574 http://nakednoiserecords.bandcamp.com/ SONGS SNIPPETS IN THIS EPISODE: “Deny” by Puke from “s/t” “Assimilation” by Ash Mouth from “s/t” “Swine Brutality (II)” by Decayed Race from “s/t” “Anti-Life” by Frame313 from “To Serve… To Protect… To Kill” “Total Overload” by Mindsnare from “Gasman” “Let the Words Roll By” by Miles Away from “Tide” “Success” by CUM from “Success” “Human Shit Grinder” by Shitgrinder from “split w/ Coffin Birth” “Disgraced” by Ill Natured from “Depth of Despair” “In the Toybox” by Diploid from “Is God Up There?” “Human Capital” by Ash Mouth from “s/t” “Consequence” by The Fevered from “Blackout” “No Blindfold” by Race Machine from “They Live, We Sleep” “Backbreaker” by Manhunt from “split w/ Internal Rot” “Soothing” by Satanic Surfers from “666 Motor Inn” “Drained” by Scumguts from “s/t” “Pointless” by Puke from “s/t” “Misery Guts” by Hurricane Death from “s/t” “Nuke G20” by Terminator 2 from “s/t demo” “Humming Milter” by Full of Hell from “collab w/ Merzbow” “Acid Death” by Avian Terror from “split w/ Wrong”

This week on Feast Yr Ears, host Harry Rosenblum is joined by Jules Opton-Himmel, owner of the Walrus and Carpenter oyster farm. Located in Charlestown, Rhode Island, Walrus and Carpenter sits on a six-acre lease in the pristine waters of Ninigret Pond. Since launching in 2009, it has grown from a small passion project to one of the most recognized brands in the state. The goal from the beginning has been to work with the best chefs and thought leaders within the culinary world, mixing hard science with a commitment to sustainable food. Tune in to hear them discuss farming oysters, how they get to market, and what happens next.

By use of a rapid technique, initial rates of D-glucose transport were obtained during the lifespan in vitro of a commercially available strain of human embryo lung fibroblasts (Flow 2000). The apparent Km of the D-glucose carrier did not change during senescence in vitro: = 1.8 mM (range 1.3-2.3) in phase II, = 1.8 mM (range 1.5-2.2) in phase III. Transport rates remained constant in stationary phase II cultures, which had completed between 30% and 80% of their replicative lifespan. A wide variation, however, was observed in terminally differentiated cells (phase III), which showed a two- to threefold increase in average cell size and protein content. In some senescent cultures, glucose transport calculated on a per cell basis was also two-to threefold increased, while it was strongly decreased (-75%) in others. When calculated per unit of cell water, protein, and surface area, respectively, transport rates in phase III cultures ranged from values established for stationary phase II cultures down to very low values. Detaching cells flushed off from senescent cultures did not show measurable rates of glucose transport into the inulin impermeable cell space. Present evidence argues against the idea that an impairment of D-glucose transport might precede loss of replicative potential in aging human fibroblasts. Instead our data indicate that the transport capacity of cell membrane finally decreases during postreplicative senescence in terminally differentiated cells.

The influence of ionising radiations on ageing is still controversial. Since Hayflick established the concept that diploid cells have finite lifespan in vitro, human diploid fibroblast (HDF) cultures have been recognised as a potent experimental model for cytogerontological investigations. In this study HDF cultures in phase II were exposed to acute irradiation with either X-rays on fast neutrons. The replicative potentials and labelling indices with [3H]thymidine were measured post irradiation until the cultures ceased growth in phase III. Cell mortality was measured by cloning. The apparent loss in replicative potential of irradiated mass cultures was wholly attributable to the loss of viable clonogenic cells. The current concept of precocious clonal senescence in vitro as a late effect of irradiation in clonogenic survivors is not supported by the present experiments. Instead, our results suggest that exposure to a single dose of ionising radiations either causes total replicative incapacitation (killing) of HDF cells and their progeny early after irradiation or leaves their replicative potentials unperturbed.