Podcasts about moiety

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Best podcasts about moiety

Latest podcast episodes about moiety

Clandestino.
Clandestino 180 - Moiety

Clandestino.

Play Episode Listen Later Mar 7, 2023 104:48


Check out Moiety at https://soundcloud.com/rugged-society

Talkin’ Solutions: Highlighting Impact Driven Companies Doing Societal Good
#52: Recognizing the importance of creating a sustainable startup for long term success - Moiety Consulting Co-Founders: Lidia Martin & Emma Walsh

Talkin’ Solutions: Highlighting Impact Driven Companies Doing Societal Good

Play Episode Listen Later Jan 4, 2023 44:54


We're talking about the importance of creating a sustainable business in the 2023 premiere of the Talkin' Solutions podcast with the co-founders of Moiety Consulting Lidia Martin and Emma Walsh. Moiety Consulting creates tailored strategies to embed sustainability into your operations and policies by addressing your company's social and environmental impact. Lidia focuses on environmental policy and supply chains while Emma maximizes your marketing efforts. In this episode we discuss:

Essex Techno Ltd Podcast
Essex Techno Limited Podcast 124

Essex Techno Ltd Podcast

Play Episode Listen Later Apr 2, 2022 128:42


Tracklist: 1. Seething Flow - Odyssey 2. Gaston Sosa & Poli Siufi - Volemos Alto 3. EANP - Supreme 4. Cream - Astral (Teleport-X Mix) 5. Alex O'Rion - Aperon 6. Moshic - Redemption 7. Juan Beldarrein - Viaje A Venus (Fabri Lopez Mix) 8. Marley Hughes - When You (Drekaan's Selfish Psycho Mix) 9. Anyawa feat. Tanmaya Bhatnagar - Roshni 10. VONDA7 - Arrivals (Cornelius SA Mix) 11. Natasha Wax & Sofia Tellovec - Lunar 12. Essco - Transition (Back To The Roots) 13. EANP - Supreme (Jelly For The Babies Mix) 14. Fehrplay - Pandemonium 15. Rafael Cerato - Sekater (2022 Revisited) 16. Brdihz - Moments 17. Rick Pier O'Niel & David Weed - Cenote (Acabus Mix) 18. Axonia - Night Falls 19. Sound Synthesis - Journey Of Life 20. Terrence Dixon - Final Results 21. J_ulio feat. Inhumind - Habanero 22. I Promised Mom feat. Aquarius Heaven - Dark Disko Mafioso (Marc DePulse Mix) 23. Roman Avan - Affectus Tenebris (Univrz Mix) 24. Unknownfunction - Breathe 25. Bruno Bazzetti - Anything Is A Mistake (Saigg Mix) 26. Gabriel Arka - These Days 27. Moiety & Manik - Gunaro (Hugh Mane Mix) 28. Bio-Dreams - White Sands (Original Mix 1993) 29. David Holmes feat. Raven Violet - It's Over, We Run Out Of Love (Darren Emerson Mix) 30. Perel feat. Marie Davidson - Jesus Was An Alien (Club Edit)

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Fourteen

Sunshine Satellite Story Podcast

Play Episode Listen Later Feb 20, 2020 14:44


The story is drawing to a close. Moiety and Akedah have faced a variety of dragons and have learned from each one a different aspect of the transformative ability of chaos. Akedah, the overt representation of a hero, is being reborn as he wakes up from a death-like state and Moiety the covert representation of a hero has risen from the pressurized depths of hell, bringing back an unknown treasure (the dragon's pearl) to combine the known threat -the (mechanical heart). Both heroes learn that their main antagonists are not each other, but forces their own psyches. "It turns out that an eerie type of chaos can lurk just behind a facade of order - and yet, deep inside the chaos lurks an even eerier type of order." ― Douglas R. Hofstadter, Metamagical Themas: Questing for the Essence of Mind and Pattern 1979 Winner of the Pulitzer Prize in non-fiction for exploring mathematical relationships in creative processes. Akedah's eyes slammed open into a body filled with pain. Moiety was crying, or more accurately, weeping hysterically with snot free-flowing from her nose.  He coughed. His mouth and nose were full of cottony silkworm thread. "You came back!" As Akedah pushed through the layered wrappings of silk threads, he felt the pins and needles of blood flowing back into his small capillaries. It made him sneeze. Moiety was trying to hug him. He stiffened. For a brief moment, he considered utilizing her repentance and joy as a way to gain the upper hand in the relationship. Now that she wanted him, he could manipulate her more easily into being compliant. He would be able to use his approval or disapproval of her as endless currency to get what he wanted out of the relationship. He gave her a cold, distant look. "What is wrong?" she asked. Akedah continued in silence. She still looked like a wet cat. He remembered his half-finished book in the high mountain city and the importance that it had placed on his motivations above his actions. He considered the fact that the quality of his life would hinge not on what he accomplished but his motivations underpinning his accomplishments. All Akedah could think of was her back to him, sailing away in his boat. He wanted revenge on this woman for the way she had unfairly treated him, but he also knew from looking at his life book, that it did not matter. He would only be right in the end if he was able to forgive her. No one in all eternity, especially not the old Morning Star, would ever care if his personal grievances were resolved to his satisfaction. The thing that would give his life meaning would be his ability to overlook the insult, to despise Shame, and choose love despite spite.  Akedah again laid it down and let it go. He would be doing a lot of that. The North Wind was long gone now, a soft, warm sunset had taken her place. Akedah stood up and promptly fell back down. Moiety rushed to help him.  "You have been asleep for three days," the Leviathan informed him. "Your strength will return." But to Moiety, he said, "Do not cling to him. He has not finished the transformation. You cannot help him in this, Princess. You must allow him to gather his own strength, or his vitality will be stunted. He must generate his fortitude from within right now, or he will forever be dependent on women for succor." With considerable effort, the Viking pulled himself up to stand using a low tree branch for support, "What is that clicking sound?" Moiety listened. She could not hear anything but ocean waves and the residual breezes left behind in the jungle canopy from North Wind's impossibly long hair. Then, she noticed it, not so much as a sound but as a nagging feeling of urgency. Softly, the feeling reached her senses as an irritating sound. The muffled metronome was present and persistent, as persistent as time. It was sending out vibrations through the jungle soil like the concentric ripples on still water... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Thirteen

Sunshine Satellite Story Podcast

Play Episode Listen Later Feb 13, 2020 16:27


Moiety had been so firmly calcified into a selfish princess, that it took the heat from the gates of hell and the pressure of the deepest trench in the ocean to soften her soul enough to be released from its binding mold. In so doing, she had, with the help of her friend, the Leviathan, defeated the ancient Nidhogg Wyrm, the titan tapeworm responsible for draining the roots of the World Tree of gratitude. However, we must not forget that the only reason that Moiety is still alive and having this adventure is that the Viking volunteered to take her place, absorbing the wrath of the chameleon's curse. In chapter thirteen, we go back in time to follow the Viking's Vision.  "We don't feel fully known, understood, or valued by others or even ourselves—that's why we labor to prove ourselves, to get people to notice us, to make a name for ourselves, or try to be someone else. Imagine how in Heaven, all this gets replaced with an unbelievable clarity of who God created you to be—fully yourself, fully unique, for a unique relationship with your Creator." ― John Burke, Imagine Heaven Akedah began to die as he absorbed the chameleon's curse from Moiety's body. The glowworms surrounded him.  "We cannot stop the curse from killing you, but we can slow it down so that you will not immediately die."  Akedah felt the venom spreading through his limbs with an unmitigated crushing pain. The glowworms were overflowing with their organic juices. They carried the Viking into a spreading Live Oak tree and began working quickly to encapsulate him in silk, a process that would curb the rise of death's steady tide.  The last thing the Viking saw was entitled princess Moiety, disembarking in his boat. Akedah closed his eyes and slept. His spirit awoke and walked through his dreams. He was in a crowded city, surrounded by masculine women and feminine men. The landscape was defined by hard angles and stone. People walked into and out of each other's personal space without greeting. There were few children and even fewer aged, only a sea of family-less persons moving without eye contact like cold fish in dark water. Every person had a number attached to them.   Akedah wandered through these streets until he saw a woman standing outside what appeared to be a tavern. She was clearly upset, and clearly in contrast with the rest of the stoic environment. She did not have a number and was therefore not allowed inside. She had two small children, a girl, and a boy.  Akedah went inside the tavern to see what was wrong. Inside the dim lounge, there was a bath occupying the majority of the room. It was lit from the inside with a bluish light. It was surrounded by tables where patrons were drinking. It would not have been so strange if it had not been full of wiggling scarabs. Some of the patrons were stuffing the scarabs in their pockets and bags. Some of them were up to their elbows playing with the bugs. Others were sitting neck deep in the bug bath.  Despite his disdain for these curious habits of the natives, the Viking, was intrigued. While Akedah watched, a beautiful woman brought him a drink. He drank. The mother outside continued to pound on the glass window. She seemed to be calling out to a big man who was happily up to his beard in the bug bath. The man was numbly ignoring her. Midway through his drink, Akedah realized that he was not feeling half as repulsed by the scarabs as he had been. In fact, they were quite mesmerizing. Some of them appeared to be made of gold. Now he understood why the patrons were putting them in their pockets. The smiling woman reappeared with another drink. He had not even finished the first drink. Something about the woman banging on the glass out front made him distrust the women inside. He finished the first drink, while he watched the scarabs glistening valuably in the blue light. They were so smooth. He wanted to take some with... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Twelve

Sunshine Satellite Story Podcast

Play Episode Listen Later Feb 6, 2020 15:02


Chapter Twelve The Viking and the Princess The Leviathan and the Princess have discovered several things in the great trench in the deepest ocean: the roots of the World Tree, the Nidhogg wyrm, and the gates of the Abyss. The Nidhogg wyrm is a giant tapeworm sucking the energy out of the roots, and the ocean floor is slowly seeping into hell. The Leviathan uses his comparatively smaller size to infect the Nidhogg at a cellular level. Moiety has discovered that she can stop the influx of the universe into her black hole heart by utilizing the creative energy of the Universal Vibration that surrounds her. However, she has lost Odin's Scroll of Poetry, and the pieces of the wyrms tale that she cut off have come to life and are attaching themselves to the World Tree in a new effort to drain the World Tree of its gratitude. "There are only two kinds of people in the end: those who say to God, "Thy will be done," and those to whom God says, in the end, "Thy will be done." All that are in Hell, choose it. Without that self-choice there could be no Hell. No soul that seriously and constantly desires joy will ever miss it. Those who seek find. Those who knock it is opened." -CS Lewis, The Great Divorce"Please tell me more about what is in the abyss," Moiety inquired of the Leviathan. "It is the waterless places," replied the Leviathan   "The waterless places?" "The void, the nothing, outer darkness where there is weeping and gnashing of teeth. It is the fire that is never content with wood, the ground that is never filled with water, and the eye that is never satiated with seeing, and it is full of the spirits who are never grateful for being." "That sounds terrible. Are there really beings that are doomed to live inside of it." "Yes. But not in the way that you might think. The gates are locked from the inside. There is no one there who has not chosen to be there.  When they first get there, they just walk in, because it has a comfortable familiarity - the spirit can walk back out, but it will not. The whole earth is filled with the Creator's glory, leaving no room left over for self-glorification. Instead of joining into its Universal vibration, cursed spirits choose to engage in self-aggrandizement, shutting themselves out of the cosmos. The Creator, in mercy, allows them to abide in the outer darkness." Moiety thought about her mother's sisters, who spent much of their time together bickering. At any time, any one of them could just get up and leave, but they seemed to thrive on the intrigue generated by their scandals. No wonder the Abyss is made of fire and full of malice. Without wood, the fire goes out. Without words, there is no gossip, and without malice present, there is no consuming hatred.  The Leviathan was listening to Moiety's thoughts. "A great chasm was set between the Heavens and the Abyss for that excellent reason. The saints would delight in extinguishing the Abyssal fire, and the Abyss dwellers would utilize that sentiment to lure them in and entrap them." Moiety and the Leviathan searched along the roots of the mountain. The Abyss continued to consume the seafloor and release its pressure on the surface in the form of volcanic activity.  Moiety could hear the malodorous thoughts of the Abyss dwellers, "It's all your fault!" A woman's voice screamed. "If you would have just listened to Me!" A man's voice thundered. "That is Mine!" "I have a right!" "This is my property! Get off My land!" "Get out of My way!" The mob's cry perspired from the crack in a sulfuric stench. Finally, the Leviathan spotted the tip of the glowing scroll peeking out of the silt. It was slowly inching its way towards the fissure. There was no way to reach it in time. It was being sucked down into the Abyss. Moiety felt terrible. The one valuable thing that she had, she was losing, and only as soon as she realized that it had any value at all. She put her... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Eleven

Sunshine Satellite Story Podcast

Play Episode Listen Later Jan 30, 2020 15:08


Chapter Eleven The Viking and the Princess The holes that the Chameleon’s zombie army had drilled into Princess Moiety’s chest would have killed her if the Viking and the glowworms had not intervened. The princess -in tune with her character- demonstrated her gratitude to the Viking for his sacrifice by stealing his boat and fleeing the island. She would have sailed home quite happy with herself if she had not had so much trouble with the holes. The holes exposed the world around Moiety to the negative pressure inside her chest. Quite a few random objects and an ocean giant were sucked inside of her to establish equilibrium. On top of that, the mermaids were attacking. The only thing the princess could think to do was to light Odin’s scroll of poetry on fire to fight them off. This caused a tsunami. Moiety also discovered that in the light of the poetry scroll, things -such as mermaids- could be seen for their true nature.  When the tsunami from the burning scroll covered the island, Moiety was sure she was going to drown. Job’s Leviathan came along at the last moment and saved her from drowning. The Leviathan carried her down to the deepest part of the ocean to the base of the island. There they meet the Nidhogg, the organism infecting the roots of the World Tree. The Nidhogg attacked Moiety and Leviathan, and while the Leviathan was reeling from the blow, the Nidhogg looked for a way to enter into the princess’ chest cavity. ____________________ “With each book I write, I become more and more convinced that the books have a life of their own, quite apart from me.” “A book, too, can be a star, explosive material, capable of stirring up fresh life endlessly, a living fire to lighten the darkness, leading out into the expanding universe.” -Madeleine L’Engle ____________________ Moiety called out to the Leviathan again. “How do I fight this?”  Her mustard seed of resistance to the darkness was enough to pierce through the current and find the dragon’s mind. Hope sliced through his despairing thoughts. The child was finally fighting. God does not give us meaningless dreams. “Tell a story,” Leviathan commanded and began to muscle himself, tiredly, toward her.  The Nidhogg had disengaged his considerable length from the root system and was hovering above the current rushing into Moiety’s chest cavity.    “Tell a story.” Moiety balked. Why? What story could she tell when all the world was rushing into her black hole heart? “What do you mean?” “The word is near you, in your mouth and in your heart.” The dragon’s words crackled into her head like a flurry of sparks jumping from a smoldering log. Maybe the Leviathan was right, and the only way to fight against the depression of consumption was through the joy of production. Perhaps the best way to fight pain was to use it to her advantage, to harness the power of the chaos around her, and use it to produce something of value. Moiety inhaled and listened. She exhaled and repeated the rhythm that she heard around her: “The ladybug is done. It splits its ruddy mold, Splaying tiny fly wings Unfurling from their fold.” The current flowing into her chest cavity slowed ever so slightly, imperceptible to anyone but Moiety. Moiety again opened her mouth to translate the universal vibration that she heard in the ocean around her. “A still small voice Is only loud to quiet ears, Tiny drips dropping Flood a city after years.” The current slowed even more. Moiety closed her eyes, a redundancy in the stygian darkness, but somehow necessary to creation. The poem continued to reveal itself. “Marching ants move Like a military fleet Tiny blades of grass Are splitting concrete. Quiet, gentle zephyrs Turn the clouds into rain. The beach expands by sand: Grain by grain.” The current in Moiety’s chest stopped, and the water in the trench... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Ten

Sunshine Satellite Story Podcast

Play Episode Listen Later Jan 23, 2020 17:19


Chapter Ten The Viking and the Princess If Moiety had understood exactly what her problems were, we could say that she had been running away from them; however, the princess was only just now learning to call things by their right name. The tsunami that resulted from lighting Odin’s scroll of poetry on fire washed the misandrogynous mermaids away but it also flooded the entire island. The weight of the giant and the anchor inside of her was pulling her down despite the scroll’s magical ability to provide some buoyancy. Job’s Leviathan has been tasked to carry the princess down into the deepest part of the ocean to confront her final dragon. “When a person doesn't have gratitude, something is missing in his or her humanity. A person can almost be defined by his or her attitude toward gratitude.” -Ellie weisel “What is this place called?” Moiety asked Leviathan.  “We have traveled up the river Gioll to the headwaters, Hevergelmir, in the Nifleheim realm.” “Nifleheim?” Moiety asked, picking one of three words she did not know. “The frozen mist, the valley of the shadow of death, and the land of the dishonored dead. The River Gioll is the current flowing through this trench - the heart of Nifelheim. It is the boundary between the living and the dead, and here at its headwater, Hevergelmir, you will meet your third and final dragon, the wyrm, Nidhogg.” Leviathan exhaled a breath of light and Moiety watched the seafloor continue to creep down under the wall. “What does this mean?” she asked. “We are at the base of the World Tree and nearby are the gates of Hel. Listen with the light of Odin’s poetry scroll.” Moiety grew quiet in the darkness and for a moment heard nothing. And then peacefully a still small voice reached her ear. It was a quiet command coming from the current directed to the fires just below the surface, “The seas have lifted up their voice The flood lifts up the pounding waves ‘Hitherto shalt thou come, but no further:  and here shall thy proud waves be stayed.’ When sorrow of death encompasses And torrents make you afraid Walking through fire you will not be burned The flame will not set you ablaze.” So it was the peace in the current’s song and not the water itself that was holding back the hellfire below the surface.  “There is more that is born in a birth than a baby, more than sound that is sung in music, more than oxygen that sustains in the breath,” said Leviathan. “And more than the wetness in water that quenches the coal,” Moiety finished the dragon’s thoughts. Moiety and the dragon continued to follow the trench against the current in the darkness. Soon Moiety began to see what looked like thick branches and fallen logs suspended in the current. Moiety saw more branches as they traveled. The branches moved fluidly with the water. They were not, in fact, branches; this was an underwater thicket of loose hanging roots. She was impressed at how the massive Leviathan was able to lithely maneuver through the underwater canopy.  As the leviathan slowed his pace, Moiety was able to get a closer look at the roots. New tendrils were sprouting off from larger ones every second. Each one ended with a small loop encircled by a larger loop. The tendrils were very small, but they grew quickly. Moiety was able to feel a warm grateful vibration when she touched them.   Intricate as coral, and moreso. Moiety could see that only some of the roots were free floating in the water. Most of the roots were knitted up in complex and creative looped cables like the sailor's woolen tunics of the far northern islands. New root systems joined old root systems to make new designs and old root systems split apart to follow new paths. The whole system was a living, moving, changing canvas of decorative knots. As vibrant as it was, something was not whole about the root system. Some of the roots were... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Nine

Sunshine Satellite Story Podcast

Play Episode Listen Later Jan 16, 2020 15:45


Chapter Nine The Viking and the Princess The Princess had stolen the Viking’s boat and left him on the island. Honestly, she did not feel too bad about the decision. That is until she discovered the holes that the chameleon and his zombified army of automatons had drilled into her chest. Unfortunately, the Princess’s chest was full of nothing, and that nothing created a negative pressure gradient which threatened shrink and suck anything around it inside of it. So far, it had inhaled an anchor, a rope, and Aipaloovek the ocean giant. This was only part of Moiety’s struggles. The mermaids had also returned. Moiety accidentally summoned a tsunami by lighting Odin’s scroll of poetry on fire which washed the mermaids away, but it also flooded the entire island. The scroll which she had carelessly lit turned out to be a light that revealed the true nature and character of the objects which were illuminated by it. “It is not the end of the physical body that should worry us. Rather, our concern must be to live while we're alive - to release our inner selves from the spiritual death that comes with living behind a facade designed to conform to external definitions of who and what we are.”Elisabeth Kubler-RossMoiety was left standing on the water above the island. She was peering down through the clear water full of salt and light - a skin diver’s delight. There were none of the expected clouds of murky silt, everything was as if it had settled that way an eon ago as quiet and observant as dinosaur fossils pressed down beneath the burden of light like a dry autumn maple leaf resting in the hymnal pages.   Moiety had the sensation of spinning like a pot on a potter’s wheel. She had been here before, but she had been a different shape. Somehow she felt that she would be back again, but not in her present form. Everything changes as it stays the same.  The scroll was keeping Moiety on top of the water, but the weight of Aipaloovik, the boat anchor, and its rigging was starting to pull her slowly beneath the water, the heavy negative pressure was still inside her chest. Quiet. For now. Moiety was buoyant, yet irresistibly sinking.  Moiety tried to stay afloat. She tucked the scroll into her waistband and pumped her arms and legs, but the inexorable heaviness in her chest was drawing her below. The danger pressed in all around, and yet Moiety could not help but notice how exorbitantly beautiful it all was. She was hovering above the island treetops, and schools of flashing fish were darting through the trees like silver shooting stars. River beds, like rocky highways into the hills, lay exposed. Giant reaching octopus and eels, freed from the boundaries of the estuary, were ascending the heights along these river bed paths, exposing their treasures. They coiled and expanded, rolled over the rocks, reaching higher with childlike intelligence. Shy seahorses, fastidious shrimp, and all manner of colorful reef fish were darting through the jungle foliage that lay closest to the surface. The island was fecund, teeming with vibrant beauty, but it was the filtered sunlight that brought it to life. Curtains of lightrays shimmered freely in and around the rocks and trees. Nothing was hidden from its joy. Everything became alive in the illumination, eerie and innocent, boldly camouflaged. Moiety felt helpless to be drowning amidst such a strong life force. She took one last breath and slipped below the waves….. Something immense erupted up from the illuminated bottom. It churned the depths like a boiling cauldron. It torpedoed through the water next to Moiety’s sinking form leaving a glistening wake of white bubbles behind it. Moiety tumbled in the water, losing her breath. The creature was coming back. Moiety could see its eyes were as red as a sunrise. It snorted a flash of light, descended, and then scooped her up on it's armored back. It rose back to the surface. The monster did not have scales, Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Eight

Sunshine Satellite Story Podcast

Play Episode Listen Later Jan 9, 2020 18:19


Chapter Eight The Viking and the Princess The Chameleon, Shame, has damaged the Princess. Her body is full of holes where it tried to remove her heart. She is alive but dying and mostly zombified. The glow worms encased her in silk in an effort to slow down encroaching death but they cannot stop it themselves. In order to save the Princess this time Akedah must take her place. Akedah has lived up to his namesake. He has volunteered to take the woman’s place inside of the silk casing. Moiety will awake on the island alone for now. “By trying to grab fulfillment everywhere, we find it nowhere.” ― Elisabeth Elliot, (https://www.goodreads.com/work/quotes/55040) Moiety was dreaming lucid dreams. She was walking on a dark red marble floor with long veins of gold. The whole slab was a single cut placed in its entirety. A gray lighted pool steamed in the center of the room that was lit all around with beeswax candles. This was her childhood home, but she was not a princess in this house. She was there as a cleaning woman. She was trying to do her job, but she also did not want anyone to recognize her. The queen walked through the room, and Moiety turned away. The ocean giant, Aipaloovik knew she was there. He was looking for her, going from window to window and peering into the room. She kept moving. The water in the pool was sloping and heaving back and forth as if in an earthquake. Moiety felt no earthquake. She tried to stay hidden. Aipaloovik appeared in the window, behind Moiety. The windowpane was not glass, it was a laminar sheet of falling water. In her dream, Aipaloovik reached through the water to grab her with his huge yellow palm. Moiety gasped and sputtered awake into the reality of a wet humid jungle.  The first thing Moiety knew was that her chest hurt.  For some reason, she was wearing Akedah’s wet wool tunic. It was itchy and uncomfortable. She was holding a parchment with some barbaric runes.  Moiety wanted to get off this creepy island. The last thing she remembered was speaking to a flashy lizard with a curious offer. Something about making her wise. What happened? Was she wise now? Where was the grouchy barbarian? Moiety knew that if she was able to make it to the coast, that she could walk around the perimeter of the island. She would eventually find the longboat. Maybe Akedah was still there. Maybe he had left her. Moiety would have left him. Moiety started towards the light. She came up close to the edge. It was a dizzying distance to the bottom. The waves lapped over the crags jutting out of the water at asperous angles. No one would survive that fall she thought. Moiety made her way back to the boat and waited a full 52 minutes before deciding to set off in the longboat by herself.  It was a beautiful day. Recent rain had pulled the edge of the heat out of the air and beautiful full clouds were lining up in rows like children waiting for a drink at the water fountain. Moiety was moving along at a quick clip and looking forward to a new adventure. Except for the deep digging pain in her chest, life seemed absolutely grand. Moiety sailed on to sunset feeling utterly self-satisfied. The sun left its glow the western sky and the stars appeared, gently at first in the east and then swelling up the sky with blinking pinpricks of joy, they became the entire field of vision. A heat storm rose in the north. The billowing castles tossed plumes of lightning back and forth highlighting their architecture in waves of lucid red and giving the impression of a momentous royal celebration. What was that pain? it seemed to be getting worse the further away from the island that she got. It was heavy. Moiety lifted her shirt to see if she could find clues on her flesh. What she found, dropped her to the floor of the boat. There were six round holes up the sides of her chest tunneling neatly into its interior. Each one was easily deep and... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Seven

Sunshine Satellite Story Podcast

Play Episode Listen Later Jan 2, 2020 15:23


It turns out that a very normal looking tropical island is not what it appears to bel. Several tunnels inside the World Tree open out into various places on the island. The island is home to an evil chameleon wyrm with a mechanized zombified army of unfortunate creatures. It is also home to an ancient race of glow worms. The evil Chameleon wyrm was in the process of removing the princess’ heart of flesh to replace it with an eternal mechanical heart when the Viking found his way out of the World Tree and onto the stage of the Chameleon’s drama. He was able to defeat the wyrm, but a piece of the dragon’s tongue attached itself to Moiety’s ankle and would not come off. “Real magic  can never be made  by offering someone else's liver.  You must tear out your own,  and not expect to get it back.” ― Peter S. Beagle, The Last Unicor (https://www.goodreads.com/work/quotes/902304) Akedah crouched to examine the oddity. It did not look as though it was hurting her, but it was stuck. He tried to slide it off. It was just tight enough to refuse to pivot over her heel.  Moiety gave a slight moan. The woman was not dead after all. The Viking felt hope gaining momentum. She opened her eyes and rolled to the side. She vomited a heave of thin yellow bile. Akedah looked at her blank eyes. “Moiety,” he called “Princess.” Nothing, just a vacuous stare. She jerked like a string toy, stood, and began to lurch back into the forest following after the hoard of mechanized animals. Akedah followed. “Moiety!” he called again. Moiety’s body gave no discernible response as it continued striving through the forest and towards the sea cliffs. Her unclothed flesh stomped unhindered through thorn and mud, catching itself on low hanging vines all the while maintaining its mechanical march. Akedah was going to have to use force to intervene. The bright beautiful ocean loomed dangerously ahead. Akedah remembered the majestic but jagged boulders at the base of the cliffs. Moiety would be certainly dashed into pulp if she stepped from the precipice.  Akedah stepped up behind her and bearhugged her over her arms, lifting her bloody feet off the earth. She did not fight. She did not resist. She did continue to mindlessly strive for the cliffs. Her body twitched side to side in tractionless gait with calm suicidal effort. Akedah thought he was going to have to tether her body to a tree. As he was wondering where he was going to find a suitable cord, a warm moist wind began to blow across the island. Akedah took his brown wool tunic off to clothe the woman. He had to pin her torso to the ground with his knee, as her body rhythmically pushed against the earth, attempting to right itself. He sat down under a broom tree and pulled her into his lap while he considered what to do. The wind was picking up. Akedah felt a rumble, that he supposed was thunder. The rumble grew stronger with the rising wind. The island’s atmosphere was absurdly normal for an epic. There was nothing on the surface that would indicate that it was a cosmic battleground, and yet here it was, complete with dragons, stars, and World Tree wormholes all set against the milieu of a normal ocean breeze and a common warm summer evening downpour.  The downpour. It all seemed so suddenly meaningless. He remembered great warriors who had fought valiantly to win difficult battles only to die weeks later from infections. He also knew well the creeping depression that settled in amongst men after victory. A warrior cannot return to the family farm as a farmer. Wolves do not live in kennels. The warm, mundane rain was relentless. The woman’s body continued to jerk mechanically. Akedah looked at her. Her eyes were glazed. Her mouth was slobbering. There was nothing in her appearance that he would desire her. He was thoroughly exasperated and wanted nothing more than to rid himself of the annoyance. He pushed the feeling... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Six

Sunshine Satellite Story Podcast

Play Episode Listen Later Dec 19, 2019 20:39


Chapter Six The Viking and the Princess “War must be, while we defend our lives against a destroyer who would devour all; but I do not love the bright sword for its sharpness, nor the arrow for its swiftness, nor the warrior for his glory. I love only that which they defend.” ― J.R.R. Tolkien, The Two Towers Lately in our story, stiff-necked Moiety has been getting jerked about quite a bit. Akedah, the Viking, has had to be on his toes to keep up with the amount of rescuing the independent princess has been requiring. She had been in trouble with her mother, an ocean giant, mermaids, and even the generous North Wind.  So far the Viking has been able to keep Moiety alive by using gifts given to him by the gods of Asgard, but when he beached the longboat on a tropical island to make repairs, she wanders off on her own. While in the jungle Moiety encountered the Chameleon, an evil lizard who is building an army of half-living half-dead mechanical-biological automatons. The princess is just what she needs to complete her menagerie of zombie foot soldiers. She has paralyzed the princess and is planning on replacing her flesh heart with a mechanical one made out of loadstone. Akedah is also deep in the jungle looking for Moiety. He has encountered the natives, ten-thousand-year-old glowworm children whose job it has always been to wrap the newly born stars in a protective casing of silk to preserve their songs as they travel into space. The children bring him to an entrance to the World Tree where he encounters Vision inside, the triune sisters Past-Present-Promise, inside one of the tunnels in the World Tree. When we left Akedah he had gone through Vision and was nearing the exit point of the tree where the Chameleon’s army was preparing to perform their grisly operation….  And so begins Chapter VI Moiety was supine in the hollow of the dead tree. She would have noticed that the inside of the tree opened up to a tunnel of considerable length if she had been able to explore, but Moiety was in too much danger. She was conscious but unable to respond to her surroundings. Only her eyes moved, and she watched as the hoard of tiny mechanized beasts swarmed over her body and began the process of slowly boring into the spaces between her ribs. They were tunneling cavities into her chest to her heart, presumably to replace it with something else. Moiety felt the pain, but it was so intense that she also felt that she was outside her body, hovering over it, watching in suspended horror. There was not much blood, but there was the terrible smell of electrocauterized flesh. She vomited. She lay in it. --------- Akedah was coming to the end of the tunnel, and he saw what he thought looked like large ants on a dead bear. He balked with abhorrence when he realized what it was. He froze while his brain whirred in an attempt to categorize the abomination. All at once he lurched, laden with emotion, at the horrific scene batting and swatting at the tiny monsters with frenzied effort. He unthinkingly beat at her motionless body. When he was able to get a grip on her drug her out into the acrid red light. The automatons followed slowly, steadily, gripping, and climbing his leg. Then, suddenly, as if a poisonous gas had taken them, they all dropped, lifeless, to the ground.  Akedah assumed that Moiety was gone, but he picked her up to carry her just as he would have carried any battle-fallen warrior: with strength and sorrow and deep conviction. This is the power of flesh. Flesh only yields to a machine in a physical sense, but in a spiritual sense, flesh and spirit are stronger than any machine. Broken human hearts keep beating, pumping right through the greatest spiritual injuries  A strange thing happens when they are broken. A broken heart creates a rift through the realms for God to come near to the brokenhearted. It cuts right through the fortified walls of... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Five

Sunshine Satellite Story Podcast

Play Episode Listen Later Dec 12, 2019 16:58


“Courage is almost a contradiction in terms. It means a strong desire to live taking the form of a readiness to die.” ― G.K. Chesterton _______________________________________________________________ The light music grew heavier. The viking thought he could smell bread baking, or maybe it was lamb meat roasting. Then he realized it was neither. The music had grown so real that he could smell it too. It smelled like a childhood memory. It smelled like home. If home was more of a home than home had been. Like the happiness of a deeply rooted family during a winter solstice festival, it smelled good. The viking plunged his hands through the water’s surface and pulled his body underneath.  The glowworms moved in a cloud of current like floating lanterns under the water. Every light was unique and beautiful, a note in the Universal Sound. Their silk trailed behind them -echoing reverberations in the flow.    Akedah floundered and gasped, but then found it easy to breathe in the water. It was as if he was not breathing on his own, but was a part of a breathing organism.    “She is here at the womb of stars. She is the ancient red dragon poised to consume the child of the laboring woman clothed in the sun.” “Stars are songs, and lies are discordant interruptions in song reverberations.” “The princess is here. She has brought her heart with her.” “She has already swept a third of the stars from the sky.” Akedah could hear the soundless voices of the shining viscid children around him like thoughts bubbling up in his own mind. “Who is she? Who is the red dragon.” Akedah wondered to himself. In unison, the voices, rolled into his head, like rushing water.  “The void.” “The nothing.” “The always winter and never Christmas.” “The waterless places.” “The outer darkness, where there is weeping and gnashing of teeth.” The viking heard all this and comprehended in a moment’s breath. “She is the fire that is never satisfied with wood.” “The ground that is never satisfied with water.” “The eye that is never satisfied with seeing.” “The spirit who is never satisfied with being.” “The consumer.” “What does she consume?” “Love.” “Joy.” “Peace.” “Patience.” “Kindness.” The viking felt a tremor of horror in his spine despite his lack of understanding of the implications of this idea.  The current was winding through an open kelp forest. Great green stipes rose to the surface like giant beanstalks, anchored and buoyant, while the sandy white bottom rolled ever on like a submerged desert. The children and the viking swirled round and round. Up and over the kelp passing seals, rockfish, and even a grey whale hiding from killer whales until they stopped by a round wooden door lying exposed in the drifting sand. Akedah grasped it by it’s heavy metal handle and hoisted it open. Akedah could see a stone stairway. It was lit with torches and spiraled steeply down into the earth. Akedah had to pull himself through the water’s surface tension into the tunnel with the same force that he used to enter the water. It was not difficult, just surprising. A cold thermocline blasted Akedah in the chest. Akedah was not at the top of a stairway. He was at the bottom of a stairway sitting on top of the water: the base of a deep well.  _______ Moiety was lying without defense at the threshold of the enemy, and the enemy had exposed her. The Chameleon was crouched in detached cold blood at her feet, her belly dragged the ground. Her eyes moved. They darted and bounced in the dim like red dice on a green casino table. Her sides heaved, and like blacksmith bellows they blew heated air into the acrid night. “si-moom si-moom si-moom,” her dry inhalations and exhalations chanted, and with this sound a fecund hoard of biologically mechanized bloodless birds and lizards swarmed into the... Support this podcast

Sunshine Satellite Story Podcast
The Viking and the Princess Chapter Four

Sunshine Satellite Story Podcast

Play Episode Listen Later Dec 6, 2019 24:24


-4- “When you light a candle, you also cast a shadow.” ― Ursula K. Le Guin ___________________________ “No! You can’t tell me what to do,” Moiety fussed. “And if you would have just listened to me, and taken me home, instead of messing around with those mermaids, my father would of had your boat fixed already.” “Your mother was trying to ‘marry’ you to an ocean troll! You can’t be serious.”  “Well, I am serious. I am going to go find someone to help us.” “Haha,” the viking laughed, “the gods go with you,” and he compartmentalized his thoughts back to boat repairs, as the Princess Moiety paraded off into the jungle.  Time dragged. Moiety felt that she must have been plodding along for hours. The interior of the island was a dark tangled jungle. The ground was soft with gripping sucking black mud, and the princess, who was quite up to her knees in the sticky stuff, had a mind to go back and tell the viking what she thought about his lack of initiative in assisting her in her escapade. So far Moiety’s mind had been bouncing up and down on a carousel of similar thoughts. She had not noticed the thick silence asphyxiating the jungle air. Moiety turned abruptly. It occurred to her that she might not know the way back to the beach, and her stomach jerked in protest as she suddenly became aware of the sucking silence permeating the foliage. “Hello!” she called out. Her voice sounded alone. Moiety turned again. The jungle could not be completely devoid of life. It was a jungle. This time scanning her environment she noticed a lizard, who, as soon as she saw it, lost its grip on the tree and plopped like overripe fruit onto the path. It laid on its side, twitching. Moiety then fully opened her eyes to her surroundings, and saw that she was surrounded by birds and lizards - each one as silent and cold as silverware - each one watching her with unblinking, unseeing orange eyes. Moiety tried to remain proud. “I’m ok, I think the beach is back this way,” she told herself. Moiety was doing fairly well at remaining calm, and was making some progress out of the jungle, until she saw the monkey. The monkey was only about a meter in length, and was hanging passively upside down on a grape vine. When the monkey saw that Moiety had finally noticed her, she widened her eyes, and smiled a grotesque counterfeit smile, flashing a mechanical maw full of thousands of thin medical needles. She calmly advanced on Moiety’s position. Terror detonated in the princess’ mind.  Moiety backed up and fled towards what, she did not know. Nighttime fully gripped the island and Moiety’s resolve to be brave. Moiety was in full panic, but still vaguely aware that if the monkey had wanted to catch her, it would have done so by now. She continued to push through the muddy brambles, spiraling ever deeper into the bowels of the jungle.  Suddenly, as if it had been spontaneously created, a bright clearing appeared ahead through the tangled vines. It glowed red with an acrid phosphorescent light, and Moiety was drawn in to it like a shrimp to an angler fish.   The clearing was larger than it had originally appeared, it's red light seemed to billow out past where Moiety assumed the ocean should have been. The ground was dry and covered with wispy grasses. A skeletal tree protruded from dead center in the parched ground, its brittle branches scratched the stark sky. The thing that captivated the senses was a dinosaur sized chameleon lazily poised amongst the limbs.   The great lizard was pinching the tree with rounded claws, and the princess felt sure that the tree should be collapsing under her scaly weight. Its face was stunningly large and Moiety was nauseated at the thought that her whole body could fit inside that cavernous dragon mouth with room to move.  The chameleon opened and closed her mouth thoughtfully, revealing a muscular lump of tongue... Support this podcast

Raw + Unfiltered Dunedin Podcast
#30 | Sam Gasson + Marze | Moiety + Creative Process

Raw + Unfiltered Dunedin Podcast

Play Episode Listen Later Oct 4, 2018 79:00


Sam Gasson trained at Otago Polytechnic spending his early career with Andrew Speigel at Edgewater resort Wanaka.   After traveling to Europe to spend time in top kitchens and working as a private chef, he then found his way to Australia spending time with Melbourne's Sand Hill Road group before moving on to executive chef for Marrawah hospitality.   Sam is now Chef-Owner of Moiety in Dunedin’s warehouse precinct, delivering an ever changing menu showcasing good shit.Facebook | Instagram   ...We love that you follow us, but our relationship can be much deeper. Let us know what you are interested in, what is happening in your part of Dunedin (and beyond) or share some joy. Celebrate someone, let us all know about secrets you discover and share the love. S/outs: Propel FItness | Toast Bar | Vanguard Specialty Coffee Co | Great Wall Takeaways | Deep South NZ Clothing

Rádio Etiópia
THE ENEMY GOD DANCES WITH THE BLACK SPIRITS

Rádio Etiópia

Play Episode Listen Later Dec 3, 2017 80:00


THE.ENEMY.GOD.DANCES.WITH.THE.BLACK.SPIRITS by tony justerini 01. Vinc2 – Chason de Soie – By The Third Sea ,2017 (00.10) 02. Helios - Equal Ourselves – Moiety, 2012 (06.25) 03. Eluvium - The Motion Makes Me Last – Similes, 2010 (09.55) 04. Roger Waters – 5.06 AM.-Vinyl,7”, Single,1984 ( 15.00) 05. Takahiro Kido – Sweet Silence – In my Time, 2007 (19.30) 06. Rogério Samora, Rodrigo, Gabriel Gomes – Poema – Frágil, 2012 (24.00) 07. Hecq – I Am You – Nght Falls , 2006 (26.30) 08. Mogway – Special N – The Revenants, 2013 (33.40) 09. Barry Guy - After the Rain: III. Antiphon – The Tree of Life OST, 2011 (36.50) 10. Anoice . Drops, The Black Rain, 2012 (39.45) 11. Takahiro Kido – The Gentle Afternoon – Fleursy Music, 2005 (43.30) 12. Eluvium – Repose in Blue, Copia, 2007 (47.20) 13. Panoptique Electrical – Some Rooms Became Us – Yes to Desire 2009 (55.30) 14. Emily Jane White – Keeley , Blood / Lines, 2013 (01.01.00) 15. Folkaolic – Niks Song – Demo, 2006 (01.06.30) 16. Roger Waters – Dejà Vu – Is This the Life you Really Want, 2017 (01.09.40) 17. Eluvium – Prelude for Time Feelers – Copia, 2007 (01.14.00) ZÈ PEDRO - 1956 - 2017 Total time : 1.20.00 h photo by Alexandre Lopes www.radioetiopia.com http://radioetiopia.phase108.net/ https://instagram.com/radioetiopia/ https://radiolisboa.pt/

BoilingPoint Radio
BPR #16 ≈ Plant Based ≈ Guest Mix by GHS (DE)

BoilingPoint Radio

Play Episode Listen Later Sep 5, 2017 53:11


1 ~ Aus Feat King Aus On The Mic (Original Mix)by Nina Kraviz 2 ~ Au Bord De La Mer by Moomin 3 ~ Café Style by Toka Project 4 ~ Chantal by Leo Pol 5 ~ Nogoodnik / Iguacu Hash by Tito Wun 6 ~ Hardmood by Oskar Offermann & Moomin 7 ~ Youre Gone (Disco/House) by Moiety and Manik 8 ~ Lyk U Use 2 (feat. Andrés) by Moodymann 9 ~ No Hurry by FYI Chris 10 ~ The Best Is Yet To Come by Sweet Fruity Brunch 11 ~ 5am by Tito Wun

plant based moiety tito wun
Divorce, Dating & Empowered Living Show
Guest Gregory Wagner - Moriteyapp

Divorce, Dating & Empowered Living Show

Play Episode Listen Later Aug 23, 2017 25:31


WGSN-DB Going Solo Network (www.goingsolonetwork.com) presents Host, Rosalind Sedacca of Divorce, Dating & Empowered Living Show with Guest, Guest, Gregory WagnerCertified Divorce Financial Analyst (CDFA), Certification in Innovation and Strategy fromHarvard Business School MBA in Finance from Fordham University in Helping Co-Parents.Gregory Wagner is a 22-year former wall-street veteran, holding various senior and global roles over his career such as Chairman and CEO of a U.S. broker dealer and Global Head of Prime Services for the Royal Bank of Scotland in the UK. Greg is currently an entrepreneur and is a founder, CEO and board member for innovative companies including:Aeonic Ventures - An innovation accelerator for startups and entrepreneurs.Leanlab – a free space where startups can record and test their innovation ideas.Moiety, Inc. – A new free innovative productivity app for families and businesses with a strong focus on helping co-parents simplify complicated schedules.

Du coup
Émission du 10 décembre 2016

Du coup

Play Episode Listen Later Dec 10, 2016


Du coup revient cette semaine avec plein d'artistes à découvrir et à re-découvrir pour votre plus grand plaisir ! On est passé d'une vibe assez chill/house pour bien commencer, en passant par une touche plus funk, pour finir avec des rythmes plus deep ! On se retrouve la semaine prochaine, pour la dernière de l'année snif snif, bisous ! L'artiste de la semaine  Otter Berry - Touch your face Le coup de coeur des artistes Ludomir - Feel The Burn L'instant anglo Jengi Beats - Flight Mode Moiety & Manik - You're Gone Daniel Dubb - Empty Pockets   Le morceau nu-disco de la semaine Forgotten Funk - The things You Do Le morceau old school George Cynnamon - House Is A Feeling L'instant franco Stone Van Brooken - Wrong Way 3 monkeyzzz - Jason Gaffner - Losing My Mind Fonkynson - Greg Kozo - One Day Le sample de la semaine Alban DSM -  Moi ça me déprime la plage La soirée de la semaine Misstress Barbara - Don't Tease Me 

Richard Vasquez Podcast
1317.Moiety:CDJLiveMixBy:RichardVasquez.aka.Dr.Love.MB

Richard Vasquez Podcast

Play Episode Listen Later Dec 27, 2014 79:54


NewMusic Weekly Report 12.27.14 1.Moiety - Dauwd 2.JustUs - SubLevel.Asadinho 3.WhatDoYouWant - MiguelMigs.MeshellNdegeocello 4.SayYouWon'tEver - Wallflower.LarryHeard 5.Stranger - MihalisSafras 6.Heat - LeighD.Oliver 7.AnotherLover - AndyCompton.Rogiers 8.HereToStay - AtJazz.JulianGomes.Zano 9.Love'sGotMeHigh - TerrenceParker.Jimpster 10.MakeRoomForMe - AntonelloFerrari.JenniferWallace.JoeyNegro 11.FallenForYou - MaffBoothroyd.JamieLewis.LaurenMason 12.StillInLove - DevonJames.AviannaAcid.JPaulGetto 13.IWantYouTonight – Mateos&Mateos.VincentInc.Asadhino The definition of "Moiety" noun (pl. moieties) formal or technical each of two parts into which a thing is or can be divided. • Anthropology each of two social or ritual groups into which a people is divided, esp. among Australian Aborigines and some American Indians. • a part or portion, esp. a lesser share. • Chemistry a distinct part of a large molecule: the enzyme removes the sulfate moiety. All of my CDJ live mixes… are made with no sequencing... no auto sync. No particular genres, no particular destination... just a smooth JOURNEY into Beats . My style is Ultra-Eclectic House Music and EDM. no matter what the flavor is. The Mix is just long enough to fit on an 80 min CD-R which you can make yourself by downloading the mix to your iTunes.... making it into playlist and then burning it to a blank CD You will find the Download click on the bottom of the link page.All tracks herein were purchased from download stores or gifted me by the artists. Please do me a few favors. Find at least one track that you love and download the track from a digital download record store… like iTunes, Traxsource or Beatport. In this day where everyone expects free music, it is the ethical thing to do to support the genius artists who create this music. The other thing I humbly would ask you to do is to click on the "Follow" box in Podomatic and if it is not asking too much… a little comment would be such an exciting reward for me. https://itunes.apple.com/us/podcast/richard-vasquez-podcast/id884539676?mt=2 http://dj.beatport.com/richard_vasquez www.richardvasquez.podomatic.com https://soundcloud.com/richard-a-vasquez http://www.mixcloud.com/richardvasquez56211/ https://www.facebook.com/richard.vasquez.56211 https://www.facebook.com/richard.vasquez.5494 http://instagram.com/djrichardvasquez https://itunes.apple.com/us/album/back-to-love-remixes/id568804548 www.richardvasquez.podomatic.com http://www.mixcloud.com/richardvasquez56211/ http://www.youtube.com/watch?v=14nHUaU_7f8 https://www.facebook.com/photo.php?v=520018921351135&set=vb.171490112475&type=2&theater http://www.thechoicerevoice.blogspot.com http://richardvasquez.podomatic.com/entry/2014-02-17T07_00_00-08_00 http://www.mixcloud.com/nicksnothome/safari-zone-episode-1/ http://www.facebook.com/photo.php?v=520018921351135&set=vb.171490112475&type=2&theater

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06
Preparation of Polyfunctional Arylmagnesium, or Arylzinc Reagents Bearing a Triazene Moiety and Their Applications in Organic Synthesis

Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 02/06

Play Episode Listen Later Feb 28, 2007


We have prepared a number of polyfunctional arylmagnesium or arylzinc reagents bearing a triazene moiety. According to some of the methodologies, we applied them in the new synthesis of functionalized carbazoles, functionalized terphenyls, and natural products such as ellipticine, and 9-methoxyellipticine.

Chemie und Pharmazie - Open Access LMU - Teil 02/02
DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety

Chemie und Pharmazie - Open Access LMU - Teil 02/02

Play Episode Listen Later Jan 1, 1991


We have previously demonstrated that transferrin-polycation conjugates are efficient carrier molecules for the introduction of genes into eucariotic cells. We describe here a more specific method for conjugation of transferrin with DNA-binding compounds involving attachment at the transferrin carbohydrate moiety. We used the polycation poly(L-lysine) or the DNA intercalator, ethidium homodimer as DNAbinding domains. Successful transferrin-receptor-mediatedd elivery and expression of the Photinus pyralis luciferase gene in K562 cells has been shown with these new transferrin conjugates. The activity of the transferrin-ethidium homodimer (TfEtD) conjugates is low relative to transferrin-polylysine conjugates; probably because of incomplete condensation of the DNA. However, DNA delivery with TfEtD is drastically improved when ternary complexes of the DNA with TfEtD and the DNA condensing agent polylysine are prepared. The gene delivery with the carbohydrate-linked transferrin-polylysine conjugates is equal or superior to described conjugates containing disulfide linkage. The new ligation method facilitates the synthesis of large quantities (>lo0 mg) of conjugates. INTRODUCTION Transferrin-polycation conjugates are efficient carriers for the uptake of DNA into eucariotic cells (I). This gene transfer technique, termed tramferrinfection, is based on receptor-mediated endocytosis of DNA complexed with polycation-transferrin conjugates (2,3). Our initial conjugate synthesis (1) involved the modification of one to two amino groups on the transferrin molecule with the bifunctional reagent succinimidyl34 2-pyridy1dithio)propionate (SPDP), followed by ligation to similarly modified polycations (polylysine or protamine) through the formation of disulfide bonds. Because there are more than 50 lysines on the large (about 80 kDa) transferrin protein, the actual site (or sites) of ligation to the polycation is unknown with this method. In this paper we describe the synthesis of new transferrin conjugates that are ligated with DNA-binding compounds in a specific manner through modification of the transferrin carbohydrate moiety. The conjugates thus obtained are free of any groups derived from chemical linking agents, since the connecting atoms are already present within the starting compounds. The carbohydrate group acts as anatural spacer that puts a 32-atom distance between the transferrin and the DNA binding moiety. This spacer effect may be important for appropriate presentation of the ligand to its receptor. As a DNA-binding compound, the polycation polylysine was used, similar to the use described in ref 1 or to the asialo-orosomucoid conjugates prepared by Wu and Wu (4). We have also prepared a novel type of transferrin conjugate that contains the DNA intercalator ethidium homodimer (5) as the DNAbinding group and demonstrate successful receptormediated gene delivery with these conjugates. EXPERIMENTAL PROCEDURES Human transferrin (iron-free), conalbumin (iron-free), and poly(L-lysine) were obtained from Sigma. Liquid chro- Abbreviations used: FITC, fluorescein ieothiocyenate; TfEtD, traneferrin-ethidium homodimer conjugate; TfpL, traneferrinpolytL- lysine) conjugate; HEPES, 4-(2-hydroxyethyl)-l-piperazineethanesulfonic acid.