Podcasts about P22

A new MP3 sermon from Antioch Presbyterian Church (PCA) is now available on SermonAudio with the following details: Title: "The Urgent Gospel, P22, Our Compassionate Shepherd" Subtitle: Mark: The Urgent Gospel Speaker: Kelley Buffaloe Broadcaster: Antioch Presbyterian Church (PCA) Event: Sunday Service Date: 7/18/2021 Bible: Mark 6:30-44 Length: 29 min.

A new MP3 sermon from Antioch Presbyterian Church (PCA) is now available on SermonAudio with the following details: Title: "The Urgent Gospel, P22, Our Compassionate Shepherd" Subtitle: Mark: The Urgent Gospel Speaker: Kelley Buffaloe Broadcaster: Antioch Presbyterian Church (PCA) Event: Sunday Service Date: 7/18/2021 Bible: Mark 6:30-44 Length: 29 min.

A new MP3 sermon from Antioch Presbyterian Church (PCA) is now available on SermonAudio with the following details: Title: "The Urgent Gospel, P22, Our Compassionate Shepherd" Subtitle: Mark: The Urgent Gospel Speaker: Kelley Buffaloe Broadcaster: Antioch Presbyterian Church (PCA) Event: Sunday Service Date: 7/18/2021 Bible: Mark 6:30-44 Length: 29 min.

www.simonstephenson.com Bio: So, I'm an author and screenwriter (and once upon a time in a galaxy far, far away I was a medical doctor). I am originally from Edinburgh in Scotland but currently live in Los Angeles, California. I've had stops in Glasgow, London and San Francisco along the way. I like all kinds of books and most kinds of movies (except horror, urgh) and love just about everything that has four legs except the chairs in my dentist's waiting room. My spirit animal is P22, our local friendly neigborhood mountain lion. I have not yet seen him, but live in daily hope. My first book, Let Not The Waves Of The Sea' was a memoir about the loss of my older brother, Dominic, in the Indian Ocean tsunami. The title comes from Khalil Gibran's book 'The Prophet', and a particular passage that resonated with me. People have told me that book is more uplifting than it sounds, but you can of course decide that for yourself if you wish. My new novel is called 'Set My Heart To Five', (that title I made up all by myself!) and it is the story of a dentist android called Jared - a 'bot' - who begins to experience feelings. The story unfolds in 2054, in a world where we humans have managed to lock ourselves out of the internet by forgetting the names of our favorite teachers and first pets. It is kind of a love letter to feelings, to the movies many of us grew up on, and the American west. So hopefully there is something for everyone... @copyright by authors on the air

https://www.simonstephenson.com Bio: So, I'm an author and screenwriter (and once upon a time in a galaxy far, far away I was a medical doctor). I am originally from Edinburgh in Scotland but currently live in Los Angeles, California. I've had stops in Glasgow, London and San Francisco along the way. I like all kinds of books and most kinds of movies (except horror, urgh) and love just about everything that has four legs except the chairs in my dentist's waiting room. My spirit animal is P22, our local friendly neigborhood mountain lion. I have not yet seen him, but live in daily hope. My first book, Let Not The Waves Of The Sea' was a memoir about the loss of my older brother, Dominic, in the Indian Ocean tsunami. The title comes from Khalil Gibran's book 'The Prophet', and a particular passage that resonated with me. People have told me that book is more uplifting than it sounds, but you can of course decide that for yourself if you wish. My new novel is called 'Set My Heart To Five', (that title I made up all by myself!) and it is the story of a dentist android called Jared - a 'bot' - who begins to experience feelings. The story unfolds in 2054, in a world where we humans have managed to lock ourselves out of the internet by forgetting the names of our favorite teachers and first pets. It is kind of a love letter to feelings, to the movies many of us grew up on, and the American west. So hopefully there is something for everyone... @copyright by authors on the air

Below are the topics covered in today's listener calls mini-episode (with timestamps). For instructions on submitting your own questions, go to calnewport.com/podcast.  - Balancing work and school. [3:34] - Deep work and ADHD. [8:28] - Building career capital as an account executive. [15:15] - Do I know Naval Ravikant? P22:35] - Art without social media. [27:31] - Pursuing a PhD purely for passion. [33:53]Thanks to Jay Kerstens for the intro music and Mark Miles for mastering.

Tony and Billy talk with Tony Lee, film producer and author of The Cat that Changed America, the story of Los Angeles' P22 mountain lion. --- Support this podcast: https://anchor.fm/wildlife-observer-network/support

Tony and Billy talk with Tony Lee, film producer and author of The Cat that Changed America, the story of Los Angeles' P22 mountain lion.

Award winning documentary filmmaker and author Tony Lee Moral tells us how we can photograph wildlife at night, and how that can help animals. Tony also tells us about the fascinating story of P 22, a mountain lion who lives in Los Angeles' Griffith Park.

He's one of the more famous pumas in LA - known as "P22" he's been living in Griffith Park for years. And in that time, Mike has been monitoring his progress. Today, he has an update.

www.simonstephenson.com/ So, I'm an author and screenwriter (and once upon a time in a galaxy far, far away I was a medical doctor). I am originally from Edinburgh in Scotland but currently live in Los Angeles, California. I like all kinds of books and most kinds of movies (except horror, urgh) and love just about everything that has four legs except the chairs in my dentist's waiting room. My spirit animal is P22, our local friendly neigborhood mountain lion. I have not yet seen him, but I live in daily hope. My first book, Let Not The Waves Of The Sea' was a memoir about the loss of my older brother, Dominic, in the Indian Ocean tsunami. The title comes from Khalil Gibran's book 'The Prophet', and a particular passage that resonated with me. People have told me that book is more uplifting than it sounds, but you can of course decide that for yourself if you wish. My new novel is called 'Set My Heart To Five', (that title I made up all by myself!) and it is the story of a dentist android called Jared - a 'bot' - who begins to experience feelings. The story unfolds in 2054, in a world where we humans have managed to lock ourselves out of the internet by forgetting the names of our favorite teachers and first pets. It is kind of a love letter to feelings, to the movies many of us grew up on, and the American west. So hopefully there is something for everyone.... VOX VOMITUS: Sometimes, it's not what goes right in the writing process, it's what goes horribly wrong. Host/Gothic Horror novelist Jennifer Anne Gordon (with help from co-hosts/authors Allison Martine and Trisha Mckee) chat with some of the best authors of the day. www.jenniferannegordon.com www.trishamckee.com www.afictionalhubbard.com/ www.patreon.com/JenniferAnneGordon

https://www.simonstephenson.com/ So, I'm an author and screenwriter (and once upon a time in a galaxy far, far away I was a medical doctor). I am originally from Edinburgh in Scotland but currently live in Los Angeles, California. I like all kinds of books and most kinds of movies (except horror, urgh) and love just about everything that has four legs except the chairs in my dentist's waiting room. My spirit animal is P22, our local friendly neigborhood mountain lion. I have not yet seen him, but I live in daily hope. My first book, Let Not The Waves Of The Sea' was a memoir about the loss of my older brother, Dominic, in the Indian Ocean tsunami. The title comes from Khalil Gibran's book 'The Prophet', and a particular passage that resonated with me. People have told me that book is more uplifting than it sounds, but you can of course decide that for yourself if you wish. My new novel is called 'Set My Heart To Five', (that title I made up all by myself!) and it is the story of a dentist android called Jared - a 'bot' - who begins to experience feelings. The story unfolds in 2054, in a world where we humans have managed to lock ourselves out of the internet by forgetting the names of our favorite teachers and first pets. It is kind of a love letter to feelings, to the movies many of us grew up on, and the American west. So hopefully there is something for everyone.... VOX VOMITUS: Sometimes, it's not what goes right in the writing process, it's what goes horribly wrong. Host/Gothic Horror novelist Jennifer Anne Gordon (with help from co-hosts/authors Allison Martine and Trisha Mckee) chat with some of the best authors of the day. www.jenniferannegordon.com www.trishamckee.com www.afictionalhubbard.com/ www.patreon.com/JenniferAnneGordon

Dejavu! No episódio de hoje, saiba como foram as grandes vitórias de Nelsinho Piquet e Ricardo Zonta, entenda a performance arrasadora de Rubens Barrichello saltando de P22 para P4 e ainda descubra quais foram os Dejavus da Corrida do Milhão. --- Send in a voice message: https://anchor.fm/daerodinamica/message

We're just not sure using Holocaust victims on your album art is the best way to remember them, ya know? Submit music to demolistenpodcast@gmail.com. Become a patron (please) at https://www.patreon.com/demolistenpodcast. Call and leave a message at (260)222-8341.Queue: Infiltrate, PM, Late Bloomer, Herbwurm, P22, Crush the Demoniac, Internal Rot, Execution Hour, Optimal Crimehttps://dontpanicitsadistro.bandcamp.com/album/a-lifelong-fighthttps://executionhour.bandcamp.com/https://ironlungpv.bandcamp.com/album/grieving-birth-lp-lungs-153https://lifelairregretrecords.bandcamp.com/album/fugitive-soundzhttps://p22p22.bandcamp.com/https://herbwurm.bandcamp.com/

Hi, everyone. Welcome back to BeProvided Conservation Radio. Today is a special edition of a live Facebook event and you can find the full video version on my Facebook or you can also find. A recorded version on YouTube. This is an abbreviated audio version of that interview with highlights about P-22 and the current vote on the California Endangered Species Act for Southern CA mountain lions.   This interview was with Beth Pratt, California regional Executive Director for the National Wildlife Federation, and co-creator of Save LA Cougars, which is a campaign to build one of the largest crossings over highway 101, one of the busiest highways in LA.   Beth is also the author of, When Mountain Lions are Neighbors: People and Wildlife Working it out in California. In this book, she shares hopeful stories from people coexisting with wildlife throughout California. Such as coyotes living in San Francisco, Grey Fox raising a family on the campus of Facebook, raptors in cities, backyard wildlife and much much more.   In the audio interview below, Beth shares stories of her background in wildlife conservation as well as the adventurous story of P-22, Hollywood's (and California's) most famous mountain lion. If you have not heard of P-22 then listen in to see how this cat is coexisting in one of the most populated area of our country within an area of only 8 square miles.  

so so sleepy.DOWNLOAD RECORDINGsubscribe to the podcast here:  http://feeds.feedburner.com/5432fun(intro by omar)Hiccup “Tides” from Imaginary EnemiesJib Kidder “All Small Scavengers” from Napkin BulletproofMANE “Bougainvilla” from S/THate Club “Passive // Agressive” from No, seriouslyRAYS “Lost In A Cage” from RAYSRamona (WA) “Lessons Learned” from Ramona & Hard Sulks SplitHard Sulks “Okay I Believe You, But My Body Just Won't” from Ramona & Hard Sulks SplitJeremy Waun “WARM SONG” from NOUMENONwarm trash “replace my” from condensedchristian fitness “footballers have feelings too” from slap bass hunksThe Cherries “Vakashin” from Self Titled 2Reptaliens “Prequel” from Prequel/ Olive BoyMARBLED EYE “FEAST” from EP 2Corbezzolo “Hey Corbo” from MidnightComfort Food “Rubber Pickles” from Waffle FrolicZach Phillips & Calvin Grad “Spiderglass” from HeadlightTotally Mild “The Next Day” from Down TimeBen Varian “4″ from this bird got a peanutfurniture “Moss” from Impish GrinEMPTY HEADS “Peeled Back” from NORMALITY$3.33 “+” from DRAFTOld Maybe “Ugly Love Me” from Piggity Pinklittle star “Calming Ritual #2″ from Little StarLoose Tooth “Day Old Glory” from Big DayWas “Reconnect” from S/TP22 “Leather Pa” from Beat Session Vol. 3FORMICA MAN “Lobster Tail For Sick Guy” from FORMICA MANMozart “The Monster” from The TickRobedoor “The Tunnel” from New Age Sewagethe spirit of the beehive “ricky (caught me tryin’)” from pleasure suckSulk “Wish I Couldn't” from Dog Swamp + morePie Face Girls “Get On The Floor” from Formative Yearsbenno, va “danger cat” from flood epLongface “The Pit of Neverending” from The Life & Death of Longface¡Socorro! “Point at Stars” from Flavor of the Day

P22 is a mountain lion that lives under the Hollywood sign in Griffith Park. He is one of the most urban large cats in the world with one of the smallest home ranges (8 mi2). As part of his research with Los Angeles carnivores, Dr. Seth Riley has been following P22 since 2012 and joins us to tell his remarkable story. The survival of P22 for over 10 years shows how large predators and people can survive together, but also highlights the challenges the Los Angeles lion population faces for the future as the remaining natural areas get more and more isolated. The story of P22 shows that there is hope for these big cats, and Seth ends with a bold proposal that could not only ensure the future of Hollywood lions, but help other species as well.

On episode #61, Wesam is joined by LODGE 49 and FOR THE PEOPLE star, OLIVIA SANDOVAL! They discuss her breakout role in TV’s FARGO, how death is not binary, P22, how she overcame her lowest moment, current events and more…Enjoy!

On episode #61, Wesam is joined by LODGE 49 and FOR THE PEOPLE star, OLIVIA SANDOVAL! They discuss her breakout role in TV’s FARGO, how death is not binary, P22, how she overcame her lowest moment, current events and more…Enjoy!

This week we welcome back Cody from Walther Arms. We discuss the entire Walther product line from the PPQ, to the PPQ SF, to the PPK, to the P22 and more. We also talk about the very unique "try before you buy" and "money back" guarantees that Walther has to offer. To learn even more, feel free to visit www.waltherarms.com.  This podcast made possible in part by Gear Websites. Check out some #TacticalLeprechaun Approved Gear Over In The Amazon Influencer Store. You can also follow or support our efforts over on the CloverTac Patreon page. Keep in touch with our monthly newsletter available over on the CloverTac Website. ---------- CloverTac Productions Original Content Credit: Christopher Dover chris.dover@clovertac.com Music Graciously Provided By: Violet Heart http://www.violetheartmusic.com

Beth Pratt-Bergstrom was inspired by the NWF’s (National Wildlife Federation) Ranger Rick magazine as a child. Now, as the executive director of the California region of the NWF, she is inspiring others to embrace nature and wildlife—and our ability to coexist in urban spaces. She and Jason met at King Gillette Ranch to discuss her experiences in nature, how P22—the cougar living in Griffith Park—changed her perspective, and why she is leading the charge on establishing a wildlife overpass in Los Angeles.   Links: savelacougars.org When Mountain Lions are Neighbors Book: https://savelacougars.myshopify.com/products/when-mountain-lions-are-neighbors-book P22 Documentary: https://thecatthatchangedamerica.com Save LA Cougars VR Experience: http://savelacougarsvr.com/ Recent Cougar Kittens: http://www.latimes.com/local/lanow/la-me-ln-new-mountain-lion-kittens-20180905-story.html   http://www.bethpratt.com Ted X Talk: https://www.youtube.com/watch?v=pMO8-f70nFY Beth’s Facebook: https://www.facebook.com/bethpratt1 P22’s Facebook: https://www.facebook.com/p22mountainlionofhollywood/   Certified Wildlife Habitat Program: https://www.nwf.org/Garden-for-Wildlife/Certify   Call us and leave a message (up to 3 minutes): 1-818-925-0106

The delightful Patrick McDonald (@patrickmcd0nald) is obsessed with the Los Angeles resident mountain lion P-22. We discuss the popularity of this celebrity feline, his diet of muledeer, raccoons and occasionally a koala (who pays us a visit), and how we should all see ourselves in P22: a displaced Mountain Lion who crossed two freeways and survived but is soldiering on in Los Angeles. We also hear a harrowing tale of Patrick encountering a pack of coyotes. Finally, we close with perhaps the most successful installation of Dolphin Chamber to date. You can see Patrick on stage at UCB Franklin Los Angeles in the one-man show “Straight White Ally” on May 17, June 5, and July 5, more info and tickets here: https://franklin.ucbtheatre.com. Catch him on Twitter @patrickmcd0nald @patrickmcd0nald. Also check out Patrick’s Jimmy Buffet podcast Parrothead Podcast on Twitter @parrotheadpod. Follow Convince Me on Twitter @ConvinceMePod and rate and review us on iTunes, pleeeeease. See acast.com/privacy for privacy and opt-out information.

The delightful Patrick McDonald (@patrickmcd0nald) is obsessed with the Los Angeles resident mountain lion P-22. We discuss the popularity of this celebrity feline, his diet of muledeer, raccoons and occasionally a koala (who pays us a visit), and how we should all see ourselves in P22: a displaced Mountain Lion who crossed two freeways and survived but is soldiering on in Los Angeles. We also hear a harrowing tale of Patrick encountering a pack of coyotes. Finally, we close with perhaps the most successful installation of Dolphin Chamber to date. You can see Patrick on stage at UCB Franklin Los Angeles in the one-man show “Straight White Ally” on May 17, June 5, and July 5, more info and tickets here: https://franklin.ucbtheatre.com. Catch him on Twitter @patrickmcd0nald @patrickmcd0nald. Also check out Patrick’s Jimmy Buffet podcast Parrothead Podcast on Twitter @parrotheadpod. Follow Convince Me on Twitter @ConvinceMePod and rate and review us on iTunes, pleeeeease. See acast.com/privacy for privacy and opt-out information.

P22 by Ilyaspeaker

Michelle wants to know some stuff about type and who Brandi looks to for inspiration for type, or letters, or typography, or.. ..lettering? It's a fun time you guys. :50 Welcome!!! Michelle’s week was super productive. She went to a wedding AND listened to an entire season of a podcast. NPR’s Invisibilia…AND binged the latest season of OITNB. Dang. 3:07 Michelle’s song for the week: Selena Gomez, Bad Liar 6:20 Brandi’s week was very similar to Michelle’s. Wedding times, 4th of July, and then it was time to straight veg. She camped out on the couch and watched a new show… Handmaids Tale, iZombie and Fear The Walking Dead. 7:00 Handmaids Tale has so many great uses of coloring, Grays and reds!

Yes, we’re the first ones to say it – both Mercedes drivers got lucky in Spa. Nico Rosberg won his 20th Grand Prix because of Lewis Hamilton’s grid penalties. In the same breath, we’ve got to agree that Hamilton was lucky to make it to the podium (from P21) thanks to the Safety Car period and the red flag. Though we wonder why Lewis Hamilton didn’t win the 44 lap Grand Prix, if 44 is his lucky number! Only 9 points separate the duo, which way will the remainder of the season go? Formula 1 returned after the summer break with a bang at Spa – it was a race of collisions and crashes (mostly into Ferraris). Was Vettel at fault at the first corner? Vettel should have a ‘How to Overtake Max Verstappen’ class with mandatory attendance for Raikkonen. Max Verstappen has been accused of dangerous driving, but of course the FIA won’t do anything about it. He’s THE crowd puller, at the moment. Though Niki Lauda believes that Max needs to visit a psychiatrist! Lauda says that he will call Jos if Max does not listen – Parent Teacher Meeting for the teenager in Monza? Also, did the young sensation not win the ‘Driver of the Day’ in Spa because his entire Orange Army was at the race track? Of course, Hamilton did find support in his Barmy Army. Force India love affair with Spa continued, though Hulkenberg may have been robbed of a podium finish. The team’s survival is in question if Sergio Perez moves teams in 2017. Maybe finishing 4th in the Constructors’ Championship will earn them the deficit. Will Kevin Magnusen be fit for Monza – and if not, will Renault make the popular decision of getting Carmen Jorda to drive? And was Fernando Alonso’s P22 to P7 more noteworthy than Hamilton’s recovery drive? With newer talent gaining popularity and faster cockpits, will Alonso have a chance for his final flourish at all? Over the summer break, we learnt that Bernie Ecclestone plans to sell Formula 1's radio messages (with a premium charge for listening to Raikkonen’s radio?). On that note, Minttu signed up for a lifetime of realtime Raikkonen humour! Hamilton hung out with Nicole over summer, and she was a real-life Pussy Cat (Doll). Also, our favourite moment of the summer break, Ricciardo calling bull-sh** on Hamilton’s tweet! Nico Rosberg has never won at Monza, will it be yet another ‘first’ for him? And will Hamilton equalize Fangio and Senna’s record for maximum poles? There’s a lot of Formula 1 this week and on our podcast, tune in! (Season 2016, Episode 27) The Inside Line F1 Podcast is hosted by Mithila Mehta and Kunal Shah. This Formula One podcast offers a unique humourous view on the sport. Follow us on Facebook: http://bit.ly/insidelineonfacebook and on Google+: http://bit.ly/insidelineongoogleplus Follow on Twitter: Mithila Mehta (http://www.twitter.com/mith_m) and Kunal Shah (http://twitter.com/kunalashah) Subscribe on iTunes Store: http://bit.ly/insidelinef1podcast RSS for Google, Android, Blackberry or Windows phones: http://bit.ly/insideline (You will need a Podcast player!) SoundCloud: http://bit.ly/insidelineonsoundcloud TuneIn Radio: http://bit.ly/insidelinef1podcast-tuneinradio MixCloud: http://www.mixcloud.com/insidelinef1podcast/ Spreaker: http://www.spreaker.com/user/insidelinef1podcast DailyMotion: http://www.dailymotion.com/inside-line-f1-podcast And hear our ever popular episodes from the previous weeks: 1. Ferrari Were Forced To Re-sign Kimi Raikkonen (http://bit.ly/29hLA7I) 2. Hamilton Must Be Pissed Off With Lauda (http://bit.ly/22r6XsP) 3. Le Mans Says No To Formula 1 (http://bit.ly/22nSUUU) 4. Max Verstappen, Talent of the Century (http://bit.ly/1OxcZWf) 5. Verstappen & Hamilton, F1's Dream Team? (http://bit.ly/29KyEJx) #F1 #Formula1 #FormulaOne #CarRacing #AutoRacing #Ferrari #Mercedes #Mclaren #Honda #RedBull #Motorsport #Motorracing #Sports #Podcast #RedBullRacing #MaxVerstappen #LewisHamilton

P5. Mrs. Brice had twenty-five mice. 布莱斯太太有25只老鼠。 P6. She fed her mice the finest cheese. 她用最好的奶酪来喂养她的老鼠。 P7. She washed and dried them behind their ears, so they were always clean. 她经常给老鼠们洗澡并把他们的耳后擦干，所以老鼠们总是非常干净。 P8. Mrs. Brice loved to sing for them. When she played the piano, twenty-four little mice danced around her. 布莱斯太太很喜欢为老鼠们唱歌。 当她弹钢琴的时候，24只小老鼠围着她跳舞。 P9. One very small mouse danced on top of her hand. He was afraid to fall between the keys. 一只非常小的老鼠在她手背上跳舞，却又害怕掉进琴键间。 P10. When Mrs. Brice went to bed, twelve little mice slept on one side of her. Twelve little mice slept on the other side. One very small mouse slept on the clock, in case he wanted to know what time it was. 当布莱斯太太躺床上睡觉时， 0 00 12只小老鼠睡在她一侧，12只小老鼠睡在她另一侧， 非常小的那只小老鼠睡在时钟上，免得他不晓得时间。 P11.In the morning, Mrs. Brice did exercises. She stretched her arms and legs. She bent over and touched her toes with her fingers. 清晨，布莱斯太太做运动。 她伸展胳膊和腿，她弯腰，用手指触碰足尖。 P12.“One, two, three, four, five, six.... One, two, three, four, five, six ....” “1,2,3,4,5,6......1,2,3,4,5,6......” P13.Twenty-four little mice did exercises too. 24只小老鼠也跟着做运动。 P14. They streched, they bent, 他们做伸展运动，他们弯腰 P15. they touched their toes. One very small mouse kept on sleeping. 碰碰自己的足尖。 非常小的那只小老鼠还在睡觉。 P16. “It it time for our walk,” said Mrs. Brice. Twelve little mice walked in front of her. Twelve little mice walked in back. One very little small mouse sat on top of Mrs. Brice hat, so he could see where they were going. P17. He saw a cat. P18. Twelve little mice ran this way. Twelve little mice ran that way. P19. One very small mouse jumped down to the ground and ran this way and that. P20. He ran so many different ways, the cat got tired of chasing him and went back. to whatever he had been doing. P21. “What a clever little mouse you are,” said Mrs. Brice. “Now we can go to buy some food.” P22. Twenty-four little mice sat in a cart and enjoyed the ride. One very small mouse sat in front. P23. They went up one aisle. P24. They went down another. P25. Mrs. Brice bought food in cans, food in jars, cold food, hot food. P26. “Now we can go home,” said Mrs. Brice. Twenty-four little mice were glad. But one very small mouse kept on leading the way. He led them to the dairy counter. P28. Mrs. Brice bought a nice, big cheese. P29. Then she and her mice went home to eat it. P30. After they ate, Mrs. Brice sang and played the piano. P31. Twenty-four mice danced around her. P32. One very small mouse kept right on eating.

The showdown you never knew you were waiting for.  The dueling histories of the Griffith Observatory and the Getty Center.

James Grieshaber on books, Emigre, P22 and his own work as a type designer.File Download (14:03 min / 13 MB)

Diese Arbeit bietet einen Einblick in die Variabilität von Phagengenomen und die Anpassungsfähigkeit des Systems Phage. Dazu wurde die modulare Architektur der Phagengenome unter verschiedenen Gesichtspunkten analysiert: • In einer natürlichen Phagenpopulation aus Naturisolaten von Salmonella typhimurium wurde die Modulzusammensetzung innerhalb einer bestimmten Gruppe von Genen, der Lysisgenkassette, untersucht. Hierbei existiert prinzipiell ein großes Variationspotential, das jedoch in der Natur bei weitem nicht in dem Maße genutzt wird, wie es auf Grund der Anzahl der existierenden modularen Allele zu erwarten wäre. Offensichtlich spielen auch Selektionsvor- oder -nachteile eines Allels oder einer Allelkombination, der zeitliche Aspekt sowie die jeweilige Isolationsbedingung des Phagen eine Rolle. • Durch Simulation von Superinfektionen unter Laborbedingungen wurde die Häufigkeit untersucht, mit der unterschiedliche superinfizierende Phagen durch Rekombination auf den Genpool eines Prophagen zurückgreifen. In diesem Zusammenhang wird die Rolle der zweiten Immunitätsregion ImmI von P22 untersucht. • Zur Untersuchung der Evolution bei Phagen im klassischen Sinn auf Nukleotidebene wurden die Gene 3 der Verpackungsregion verschiedener lambdoider Phagen analysiert. Insgesamt zeigt sich, dass trotz des enormen Variationspotentials, das den lambdoiden Phagen aufgrund der Vielzahl der nachgewiesenen Allele in den einzelnen Kassetten zur Verfügung steht, insgesamt bis heute nur ein geringer Teil der theoretisch denkbaren Modulkombinationen nachgewiesen werden konnte.