Podcasts about oocyte

Take a sneak peak at this month's Fertility & Sterility! Articles discussed this month are:   4:08 Classification system of human ovarian follicle morphology: recommendations of the National Institute of Child Health and Human Development - sponsored ovarian nomenclature workshop  12:32 Impact of Prednisone on Vasectomy Reversal Outcomes (iPRED Study): Results from a Randomized, Controlled Clinical Trial 21:38 Triggering oocyte maturation in IVF treatment in normal responders: a systematic review and network meta-analysis 33:57 Parental Balanced Translocation Carriers do not have Decreased Usable Blastulation Rates or Live Birth Rates Compared to Infertile Controls  45:28 A re-look at the relevance of TSH and thyroid autoimmunity for pregnancy outcomes: Analyses of RCT data from PPCOS II and AMIGOS View Fertility and Sterility May 2025, Volume 123, Issue 5:  https://www.fertstert.org/issue/S0015-0282(25)X0004-2 View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peek at this month's Fertility & Sterility! Articles discussed this month are:   01:47 Puberty progression in girls with Turner syndrome after ovarian tissue cryopreservation 14:55 Optimal Restoration of Spermatogenesis following Testosterone Therapy using hCG and FSH 27:58 Human embryos with segmental aneuploidies display delayed early development: a multi-centre morphokinetic analysis 39:56 Neurodevelopmental or behavioural disorders in children conceived after assisted reproductive technologies: A nationwide cohort study 45:23 Efficacy and safety of estetrol (E4) 15 mg/drospirenone (DRSP) 3 mg combination in a cyclic regimen for the treatment of primary and secondary dysmenorrhea: A multicenter, placebo-controlled, double-blind, randomized study 51:13 Ovulation trigger versus spontaneous LH surge on live birth rate following frozen embryo transfer in a natural cycle: a randomized controlled trial 60:29 A Cost Analysis of Clomiphene Citrate, Letrozole and Gonadotropin with Intrauterine Insemination using Outcome Data from the AMIGOS Trial   View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peek at this month's Fertility & Sterility! Articles discussed this month are:   01:46 It is time to rethink coordination of fresh oocyte retrievals with microscopic testicular sperm extraction 02:55 Rescue intracytoplasmic sperm injection improved cumulative live birth rate for cycles with second polar body extrusion rate

Take a sneak peek at this month's Fertility & Sterility! Articles discussed this month are:   01:03 (Not) My body, my choice? - Should physicians be facilitating gestational carrier arrangements in the absence of medical indication? 05:22 Morphological changes of endometriomas during pregnancy and after delivery detected using ultrasound 12:17 Hormone-free vs. follicle-stimulating hormone–primed infertility treatment of women with polycystic ovary syndrome using biphasic in vitro maturation: a randomized controlled trial 24:37 Association of in vitro fertilization with severe maternal morbidity in low-risk patients without comorbidities 33:59 The association between primary ovarian insufficiency and increased multimorbidity in a large prospective cohort (Canadian Longitudinal Study on Aging) 41:28 Target trial emulation of preconception serum vitamin D status on fertility outcomes: a couples-based approach 52:59 Predicting a successful match among applicants to reproductive endocrinology and infertility fellowship View Fertility and Sterility February 2025, Volume 123, Issue 2:https://www.fertstert.org/issue/S0015-0282(24)X0015-1 View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peek at this month's Fertility and Sterility! Articles discussed this month are:   02:59 Preimplantation genetic testing for aneuploidy is associated with reduced live birth rates in fresh but not frozen donor oocyte in vitro fertilization cycles: an analysis of 18,562 donor cycles reported to Society for Assisted Reproductive Technology Clinic Outcome Reporting System 19:37 Sperm concentration remains stable among fertile American men: a systematic review and meta-analysis 26:02 Preovulation body mass index and pregnancy after first frozen embryo transfer in patients with polycystic ovary syndrome and insulin resistance 36:54 A retrospective comparison of the impact of industry payments on assisted reproductive technology practice and outcomes 44:55 Preimplantation genetic testing for aneuploidy in unexplained recurrent pregnancy loss: a systematic review and meta-analysis 61:03 Initiation and outcomes of women pursuing planned fertility preservation 65:49 First successful ovarian cortex allotransplant to a Turner syndrome patient requiring immunosuppression: broad implications   View Fertility and Sterility October 2025, Volume 123, Issue 1: https://www.fertstert.org/issue/S0015-0282(24)X0014-X View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peek at this month's Fertility and Sterility! Articles discussed this month are:   01:48 Trustworthiness criteria for meta-analyses of randomized controlled studies: OBGYN journal guidelines 06:24 Playing the long game for reproductive rights 08:04 Limiting the number of fresh donor oocytes inseminated with sperm as a strategy to minimize supernumerary embryos 15:38 Effectiveness of degradable polymer film in the management of severe or moderate intrauterine adhesions (PREG-2): a randomized, double-blind, multicenter, stratified, superiority trial 26:37 Aneuploidy rates and likelihood of obtaining a usable embryo for transfer among in vitro fertilization cycles using preimplantation genetic testing for monogenic disorders and aneuploidy compared with in vitro fertilization cycles using preimplantation genetic testing for aneuploidy alone 38:13 Antimüllerian hormone levels are associated with time to pregnancy in a cohort study of 3,150 women 47:50 Racial disparities in the outcomes of euploid single frozen-thawed embryo transfer cycles – analysis of the Clinical Outcome Reporting System of the Society for Assisted Reproductive Technology 2016–2018 data 57:14 No association between celiac disease and female infertility: evidence from Mendelian randomization analysis   View Fertility and Sterility October 2024, Volume 122, Issue 6:  https://www.fertstert.org/issue/S0015-0282(24)X0013-8 View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peek at this month's Fertility and Sterility! Articles discussed this month are:   04:20 LGBTQ+ family building: progress but lots more to do/Therapeutic donor insemination for LGBTQ+ families: a systematic review 09:52 Confirmation and pathogenicity of small copy number variations incidentally detected via a targeted next-generation sequencing-based preimplantation genetic testing for aneuploidy platform 23:40 Trends and outcomes of fresh and frozen donor oocyte cycles in the United States 34:11 A survey study evaluating donor gamete utilization rates, patient satisfaction, and fertility treatment outcomes according to desired race and ethnicity 39:29 Racial and ethnic disparities in wait times for donor oocytes 41:16 Return rates and pregnancy outcomes after oocyte preservation for planned fertility delay: a systematic review and meta-analysis 49:23 Longer duration to optimal endometrial thickness in women with premature ovarian insufficiency is associated with clinical pregnancy rate in donor egg cycles 56:30 Feasibility and efficacy of a subcutaneous catheter for controlled ovarian stimulation 01:00:23 International Committee for Monitoring Assisted Reproductive Technology world report: assisted reproductive technology, 2015 and 2016 View Fertility and Sterility October 2024, Volume 122, Issue 5:  https://www.fertstert.org/issue/S0015-0282(24)X0012-6 View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peek at this month's Fertility & Sterility! Articles discussed this month are:   03:06 Embryos derived from single pronucleus are suitable for preimplantation genetic testing 09:59 Clinical factors impacting microdissection testicular sperm extraction success in hypogonadal men with nonobstructive azoospermia 24:46 Educational gradients in the prevalence of medically assisted reproduction births in a comparative perspective 32:14 Minimum number of mature oocytes needed to obtain at least one euploid blastocyst according to female age in in vitro fertilization treatment cycles 40:02 In vitro fertilization and perinatal outcomes of patients with advanced maternal age after single frozen euploid embryo transfer: a propensity score-matched analysis of autologous and donor cycles 46:57 Imprinting disorders in children conceived with assisted reproductive technology in Sweden View Fertility and Sterility October 2024, Volume 122, Issue 4:  https://www.fertstert.org/issue/S0015-0282(24)X0010-2 View Fertility and Sterility at https://www.fertstert.org/  

Fertility and Sterility On Air brings you the best of ASRM 2024! In Part 2, our hosts bring you: home semen testing with Dan Greenberg (0:40), state insurance mandates for fertility coverage with Adeola Adeyeye (7:13), resource utilization difference between programmed and natural transfers with Ben Peipert (12:23), patient perspectives on embryo donation with Deb Roberts (21:37), corpus lutea and preeclampsia risk after embryo transfer with David Huang (35:02), exercise during stimulation with Maren Shapiro (40:57), combination of letrozole and clomiphene with Rachel Mejia and Jessica Kresowik (49:07), the impact of Alabama's personhood bill with David Monroe (54:06), and embryo quality and polygenic risk with Jordan O'Donnell (58:40). View Fertility and Sterility at https://www.fertstert.org/  

Fertility and Sterility On Air brings you the best of ASRM 2024! In Part 1, our hosts bring you: in vitro maturation with Christian Kramme (1:40), uterine transplant data updates with Leigh Ann Humphries (9:21), novel characterization of ploidy abnormalities with Ludovica Picchetta (21:50), maternal age and euploid transfer success with Philip Romanski (24:45), ultrasound assessment of ovarian stiffness to evaluate reproductive aging with Elnur Babayev (32:45), cost effectiveness of PGT-A in good prognosis patients with Ariel Dunn and Josh Combs (42:33), long-term follow up of oocyte donors with Jerrine Morris (51:16), and combining platforms to improve identifiation of mosaic embryos with Christopher Weier (58:28). View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peek at this month's Fertility & Sterility! Articles discussed this month are:   02:17 In vitro fertilization practice in patients with absolute uterine factor undergoing uterus transplant in the United States 21:22 Classification and treatment of vaginal strictures at the donor-recipient anastomosis after uterus transplant 31:18 Impact of time interval from cesarean delivery to frozen embryo transfer on reproductive and neonatal outcomes 37:37 Multiplexed serum biomarkers to discriminate nonviable and ectopic pregnancy 50:20 Urinary benzophenone-3 concentrations and ovarian reserve in a cohort of subfertile women 53:38 Assessment of pregnancy outcomes in donor oocyte thaw cycles comparing fresh embryo transfer to cryopreserved-thawed embryo transfer: a sibling oocyte study View Fertility and Sterility September 2024, Volume 122, Issue 3:https://www.fertstert.org/issue/S0015-0282(24)X0009-6 View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peak at this month's Fertility & Sterility! Articles discussed this month are:   04:19 Assessment of obstetric characteristics and outcomes associated with pregnancy with Turner syndrome 13:55 Effect of postthaw change in embryo score on single euploid embryo transfer success rates 22:38 Effectiveness of preconception weight loss interventions on fertility in women: a systematic review and meta-analysis 37:17 Functional evidence for two distinct mechanisms of action of progesterone and selective progesterone receptor modulator on uterine leiomyomas 43:47 Predicting risk of endometrial failure: a biomarker signature that identifies a novel disruption independent of endometrial timing in patients undergoing hormonal replacement cycles 54:28 Analysis of factors affecting the prognosis of patients with intrauterine adhesions after transcervical resection of adhesions 01:05:29 Effects of a eucaloric high-fat diet on anterior pituitary hormones and adipocytokines in women with normal weight View Fertility and Sterility August 2024, Volume 122, Issue 2: https://www.fertstert.org/issue/S0015-0282(24)X0008-4 View Fertility and Sterility at https://www.fertstert.org/  

Fertility and Sterility On Air brings you the best of ESHRE 2024! In Part 1, hosts Micah Hill, Paul Pirtea, and Kate Devine bring you: embryo reexpansion and live birth with Ibrahim Elkhatib (01:30), a discussion with the RBMO editor, Nick Macklon, and F&S editor, Kurt Barnhardt, about “IVF add-ons” (11:21), rescue in vitro maturation with Danilo Cimadomo (24:43), discussion with F&S On Air listener Elizabeth Glanville (31:38), one side of the debate on dual/double triggers with Raoul Orvieto (35:27), and cross-border reproductive care for same-sex male intended parents with Brent Monseur (48:59).  View Fertility and Sterility at https://www.fertstert.org/  

Fertility and Sterility On Air brings you the best of ESHRE 2024! In Part 2, hosts Micah Hill, Paul Pirtea, and Kate Devine bring you: progesterone levels in a randomized controlled trial with long-acting FSH with Annalisa Racca (01:19), live birth of day 7 embryo transfers with Marcela Colonge and Nicolas Garrido (10:57), an ICSI vs conventional IVF randomized controlled trial with Sine Berntsen and Nina la Cour Freiesleben (17:17), limitations of reporting mosaicism in PGT-A with Dhruti Barbariya and Antonio Capalbo (27:15), interview with ASRM president Paula Amato and president-elect Elizabeth Ginsburg (32:41), and ovarian aging with Kutluk Oktay (45:17).   View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peek at this month's Fertility & Sterility! Articles discussed this month are:   4:56 A pilot study to investigate the clinically predictive values of copy number variations detected by next-generation sequencing of cell-free deoxyribonucleic acid in spent culture media 18:17 The impact of microfluidics sperm processing on blastocyst euploidy rates compared with density gradient centrifugation: a sibling oocyte double-blinded prospective randomized clinical trial 25:20 Prediction of pregnancy-related complications in women undergoing assisted reproduction, using machine learning methods 40:36 The effect of laser-assisted hatching on vitrified/warmed blastocysts: the ALADDIN randomized controlled trial 50:49 Assessment of clinical pregnancies in up to eight ovarian stimulation with intrauterine insemination treatment cycles in those unable to proceed with in vitro fertilization 1:02:56 Comparative analysis of pregnancy outcomes in preimplantation genetic testing for aneuploidy and conventional in vitro fertilization and embryo transfer: a stratified examination on the basis of the quantity of oocytes and blastocysts from a multicenter randomized controlled trial 1:09:45 Predictive models of miscarriage on the basis of data from a preconception cohort study   View Fertility and Sterility July 2024, Volume 122, Issue 1: https://www.fertstert.org/issue/S0015-0282(24)X0006-0 View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peak at this month's Fertility & Sterility! Articles discussed this month are:   4:33 A randomized controlled trial to compare the live birth rate of the first frozen embryo transfer following the progestin-primed ovarian stimulation protocol vs. the antagonist protocol in women with an anticipated high ovarian response 20:24 Improved fertilization, degeneration, and embryo quality rates with PIEZO–intracytoplasmic sperm injection compared with conventional intracytoplasmic sperm injection: a sibling oocyte split multicenter trial 31:15 A comparison of pregnancy outcomes and congenital malformations in offspring between patients undergoing intracytoplasmic sperm injection and conventional in vitro fertilization: a retrospective cohort study 37:05 A follicular volume of >0.56 cm3 at trigger is the cutoff to predict oocyte maturity: a starting point for novel volume-based triggering criteria 47:35 Midluteal serum estradiol levels are associated with live birth rates in hormone replacement therapy frozen embryo transfer cycles: a cohort study 57:10 First pregnancy and live birth from ex vivo-retrieved metaphase II oocytes from a woman with bilateral ovarian carcinoma: a case report 1:00:56 Associations between race and ethnicity and perioperative outcomes among women undergoing hysterectomy for adenomyosis   View Fertility and Sterility June 2024, Volume 121, Issue 6: https://www.fertstert.org/issue/S0015-0282(24)X0005-9   View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peek at this month's Fertility and Sterility! Articles discussed this month are:  6:24 Iatrogenic and demographic determinants of the national plural birth increase 18:10 Comparison of outcomes between intracytoplasmic sperm injection and in vitro fertilization inseminations with preimplantation genetic testing for aneuploidy, analysis of Society for Assisted Reproductive Technology Clinic Outcome Reporting System data 24:54 Retrospective cohort study comparing the success of medical management of early pregnancy loss in pregnancies conceived with and without medical assistance 33:31 Association of duration of embryo culture with risk of large for gestational age delivery in cryopreserved embryo transfer cycles 38:32 Endometriosis diagnosed by ultrasound is associated with lower live birth rates in women undergoing their first in vitro fertilization/intracytoplasmic sperm injection treatment 47:29 Quantitative ultrasound measurement of uterine contractility in adenomyotic vs. normal uteri: a multicenter prospective study 53:13 Unhealthy air quality secondary to wildfires is associated with lower blastocyst yield 1:03:22 The American Society for Reproductive Medicine's new and more inclusive definition of infertility may conflict with traditional and conservative religious-cultural values  View Fertility and Sterility May 2024, Volume 121, Issue 5: https://www.fertstert.org/issue/S0015-0282(24)X0004-7 View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the use of ICSI (4:51), fertility treatments among reproductive-aged women after cancer (7:43), oxygen tension during in vitro maturation (18:45), international gestational surrogacy in the United States (30:31), the menstrual cycle and weekly and lunar rhythms (39:42), AMH predicts ovulation in women with PCOS treated with clomid and metformin (46:29), laparoscopic Davydov vs laparoscopic Vecchietti neovaginoplasty in women with Mayer-Rokitansky-Kuster-Hauser syndrome (58:32), and vNOTES retroperitoneal transient uterine artery occlusion (1:04:47). View Fertility and Sterility Volume 121, issue 4: https://www.fertstert.org/issue/S0015-0282(24)X0003-5 View Fertility and Sterility at https://www.fertstert.org/

Listen to this interview featuring Dr. Jeremy Applebaum, who recently published "Impact of coronavirus disease 2019 vaccination on live birth rates after in vitro fertilization"  Read the article  https://www.fertstert.org/article/S0015-0282(23)02029-0/abstract View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peak at this month's Fertility and Sterility! Topics this month include the impact of COVID-19 vaccination on live birth rates after IVF (4:17), the "freeze-all" strategy in women with adenomyosis (13:47), the impact of adenomyosis on donor egg IVF cycles (24:54), ART associated risk factors for retained products of conception (30:00), the POPI-plus tool (38:14), the association between job control and time to pregnancy in a preconception cohort (49:10), and sperm retrieval outcomes of contralateral testis in men with nonobstructive azoospermia and unsuccessful unilateral microdissection testicular sperm extraction (58:30).  View Fertility and Sterility Volume 121, issue 3: https://www.fertstert.org/issue/S0015-0282(24)X0002-3 View Fertility and Sterility at https://www.fertstert.org/  

Listen to this interview featuring Dr. Papri Sarkar and Dr. Phillip Romanski, who discuss their recent article "Optimal antimüllerian hormone levels in oocyte donors: a national database analysis."  Read the article  https://www.fertstert.org/article/S0015-0282(23)01992-1/abstract  View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peek at this month's Fertility and Sterility! Topics this month include the optimal AMH level in oocyte donors (5:23), the role of the mean number of DNA breakpoints (MDB) in sperm DNA integrity and IVF outcomes (12:29), declining FET outcomes with increasing female BMI (22:38), higher live birth rates in normal BMI PGT-A FET cycles (30:33), the association between oocyte donors' or recipients' BMI with clinical outcomes (40:49), isthomocele and IVF success (48:05), and quantifying the variability in the assessment of reproductive hormone levels (56:29). View Fertility and Sterility Volume 121, issue 2: https://www.fertstert.org/issue/S0015-0282(23)X0017-X View Fertility and Sterility at https://www.fertstert.org/

We are all the product of a reproductive process, yet reproductive biology, or the study of the processes and mechanisms involved in reproduction, is not well understood. Deepening our understanding of reproductive biology is crucial to advancing assistive reproductive technologies (ART) and advancing our collective comprehension of inheritance and evolution. Our guests for this episode are a couple, and we mean a literal married couple, of reproductive biology experts. Dr. Pavla Brachova and Dr. Nehemiah Alvarez, both working in the Eastern Virginia Medical School's Department of Physiological Sciences. In their collaborative work they aim to better understand and characterize the role of RNA and cellular events that impact ovarian function in women. We learn about their work with oocytes, which are single cells that grow and mature within the ovary and once fertilized provide the foundations of an embryo capable of maturing to a new individual. They outline how they use digital PCR (dPCR) and other methods to monitor RNA regulation in single cells and how progressing this work and lead to potential RNA-based therapies. In Cassie's career corner we hear childhood stories from each guest and learn about their respective career paths, which eventually collided and merged. They share insights on the importance of having mentors experienced in your field, the challenges of shared job searching, and the joys of collaborating as a couple with shared scientific interests.Visit the Absolute Gene-ius page to learn more about the guests, the hosts, and the Applied Biosystems QuantStudio Absolute Q Digital PCR System. 

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the association between thick endometrium with hypertensive disorders of pregnancy (3:40), state insurance mandates and racial ethnic inequities in ART (12:45), azoospermia factor c microdeletions and outcomes of art (18:20), sperm production in men with azoospermia factor c Y-chromosome microdeletions (25:51), orthotopic and heterotopic ovarian tissue transplantation outcomes (31:25), endocrine profile of the kisspeptin receptor agonist MVT-602 in healthy premenopausal women (38:33), and risk of hysterectomy at time of myomectomy (47:30).  View Fertility and Sterility Volume 121, issue 1: https://www.fertstert.org/issue/S0015-0282(23)X0016-8  View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peak at this month's Fertility & Sterility! Topics this month include rebiopsy and analysis of noneuploid embryos with 2 distinct PGT platforms (6:01), mobile phone use and semen quality (25:22), steroid use and fertility outcomes (33:54), estradiol levels during programmed frozen embryo transfer cycles (43:25), gender differences in letters of recommendation for REI fellowship (50:38) and hysteroscopic morcellation vs curettage for retained products of conception (1:02:57)  View Fertility and Sterility Volume 120, issue 6: https://www.fertstert.org/issue/S0015-0282(23)X0015-6 View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peek at this month's Fertility & Sterility! Topics this month include scientific integrity (1:59),  chromosomal, gestational, and neonatal outcomes of mosaic embryos (6:26), risk of congenital malformations after ICSI (15:15), successful cryptozoospermia management with multiple semen specimen collection (28:58), and fertility preservation in girls with Turner syndrome (39:03)  View Fertility and Sterility Volume 120, issue 5:  https://www.fertstert.org/issue/S0015-0282(23)X0014-4  View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peak at this month's Fertility & Sterility! Topics this month include whether sperm quality affects reproductive outcomes (2:20), CRISPR for human embryonic editing (6:00), the appropriate length of REI training (12:28), meeting the US demand for fertility services (14:44), testosterone and LH as predictors of semen parameter improvement in men treated with anastrazole (25:35), reproductive inequity and IUI outcomes in patients with limited English proficiency (41:33), the developmental potential of mature oocytes derived from rescue IVM (50:28), and trends in infertility prevalence and treatment seeking in the US (59:19).  View Fertility and Sterility Volume 120, issue 4:  https://www.fertstert.org/issue/S0015-0282(23)X0013-2  View Fertility and Sterility at https://www.fertstert.org/    

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the promise and peril of using ChatGPT as a fertility counseling tool (5:55), the impact of elevated serum progesterone levels before FET on reproductive outcomes (21:10), Bologna vs Poseidon criteria as predictors of the likelihood of obtaining at least 1 euploid embryo in poor ovarian response (28:44), the association of environmental exposure with decreased male fertility, and oral vinorelbine to treat ectopic pregnancy (45:18).  View Fertility and Sterility Volume 120, issue 3: https://www.fertstert.org/issue/S0015-0282(23)X0011-9   View Fertility and Sterility at https://www.fertstert.org/  

A new editorial paper was published in Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 15, Issue 15, entitled, “Epigenetic aging in oocytes.” Aging-related phenotypes span many different tissues and cell types, and start to occur at different ages - a different typical age for every cell type. In their new editorial, researchers Peera Wasserzug-Pash and Michael Klutstein from The Hebrew University of Jerusalem discuss one of the earliest occurring aging events in the human body, which is the beginning of female reproductive aging and deterioration. The clinical cut-off for advanced maternal age (AMA), a condition associated with poor reproductive outcomes, is 35 years old. “The early onset of reproductive aging poses a significant challenge to clinicians since a global consistent increase in maternal age at first birth has occurred in recent decades, effectively shortening the available time window for reproduction [1].” As the rate of patients with advanced maternal age rises, and with it, the number of patients in fertility clinics, so does the necessity for a fundamental understanding of the reproductive aging process. In recent years, it has been established that there is a substantial dominating influence of oocyte quality loss on age-related fertility decline. This is best demonstrated by the rise in IVF success rates in reproductively aged women when they receive an egg donation from a younger woman. Oocyte quality loss is characterized by diminished cellular function and an increased occurrence of chromosomal nondisjunctions. “Our recent publication [4] addresses the question of additional, epigenetic mechanisms that lead to the occurrence of age-related oocyte quality loss.” DOI - https://doi.org/10.18632/aging.204976 Corresponding author - Michael Klutstein - michaelk@ekmd.huji.ac.il Video short - https://www.youtube.com/watch?v=KHOVKKaJykY&t=45s Sign up for free Altmetric alerts about this article - https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.204976 Subscribe for free publication alerts from Aging - https://www.aging-us.com/subscribe-to-toc-alerts Keywords - aging, oocytes, heterochromatin, epigenetics, maturation About Aging-US Launched in 2009, Aging-US publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging-US go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways. Please visit our website at https://www.Aging-US.com​​ and connect with us: SoundCloud - https://soundcloud.com/Aging-Us Facebook - https://www.facebook.com/AgingUS/ Twitter - https://twitter.com/AgingJrnl Instagram - https://www.instagram.com/agingjrnl/ YouTube - https://www.youtube.com/@AgingJournal LinkedIn - https://www.linkedin.com/company/aging/ Pinterest - https://www.pinterest.com/AgingUS/ Media Contact 18009220957 MEDIA@IMPACTJOURNALS.COM

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the effect of maternal age on ART success rates after euploid embryo transfer (8:42), attachment of trophoblastic spheroid onto endometrial epithelial cells (18:25), antagonist protocol vs flare in poor responders (26:39), the association between dietary patterns and risk of miscarriage (37:32), and the assessment of foliiculogenesis in ovarian tissue from young patients with Turner syndrome (43:37).  View Fertility and Sterility Volume 120, issue 2: https://www.fertstert.org/issue/S0015-0282(23)X0009-0   View Fertility and Sterility at https://www.fertstert.org/  

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the impact of socioeconomic factors on semen parameters (01:42), modified natural versus programmed embryo transfer cycles (09:45); gynecologic malignancy after treating fibroids with interventional radiology procedures (20:41); state insurance mandates on disparities on donor oocytes (28:35); vasectomy incidence rates after the reversal of Roe v. Wade (38:19). View Fertility and Sterility Volume 120, issue 1: https://www.fertstert.org/issue/S0015-0282(23)X0007-7  View Fertility and Sterility at https://www.fertstert.org/  

Fertility & Sterility comes to you from ESHRE 2023 in Copenhagen, Denmark! Listen in as we discuss some of the best abstracts from the conference. Topics include the composition of commercially available human preimplantation embryo culture media (Miriam Zagers - 01:12); a trial of intraovarian injection of platelet rich plasma for women with poor ovarian response (Nola Herlihy - 09:49); the diagnostic yield of custom-designed gene panel for patients with premature ovarian insufficiency (Asma Sassi - 18:06); a trial treating IVF patients with abnormal vaginal microbiome (Thor Haahr - 28:03); peri- and postnatal outcomes three years of life after the transfer of mosaic embryos (Ruth Morales - 36:33); a teen survey on sex and reproductive health education in the UK (Rina Biswakarma - 42:10); a comparison of fetal ploidy status in pregnancy loss evaluated by cell-free fetal DNA in maternal blood versus direct sequencing of the pregnancy tissue (Tanja Schlaikjær Hartwi - 48:12) and clustering identifies to establish distinct subtypes of PCOS (Kim van der Ham - 57:02) View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peak at this month's Fertility & Sterility! Topics this month include a trial on maternal spindle transfer for recurrent implantation failure (02:38), progesterone rescue during embryo transfer (15:07), contraceptive-specific AMH (21:58), soy and ovarian reserve (33:20), micronutrient supplementation on time-to-conception and clinical pregnancy rate (38:08), effects of large, non-distorting fibroids (44:41) and a look at Letters to the Editor (52:30). View Fertility and Sterility Volume 119, issue 6: https://www.fertstert.org/issue/S0015-0282(23)X0006-5  View Fertility and Sterility at https://www.fertstert.org/  

This week we're talking about "advanced maternal age" which is the medical term for women having babies over the age of 35. The real problem here comes down to oocyte aging, or aging eggs, which is related to poor ovarian reserve. We'll talk about why egg quality declines with time, what sort of testing your doctor will run, the difference between poor ovarian reserve and advanced maternal age, the options for women trying to get pregnant, lifestyle and supplements for oocyte aging and to increase egg quality, and also advanced paternal age, or old sperm. 00:00 - Intro 00:28 - Why egg (oocyte) quality declines with time 03:07 - Testing for oocyte quality 04:56 - Poor ovarian reserve vs. advanced maternal age 06:57 - Options for women with oocyte aging 08:36 - Lifestyle and supplements for oocyte aging 11:11 - Advanced paternal age 13:49 - Outro Here's the video version of Dr. Kate and Dr. Amy talking this one through. If you're working on your health by balancing gene SNPs and epigenetics, join Genetic Rockstars. If you'd like to take a course on MTHFR, click here. To learn more about Dr. Kate Naumes, visit naumesnd.com. For more about Dr. Amy Neuzil, visit tohealthwiththat.com --- Send in a voice message: https://podcasters.spotify.com/pod/show/tohealthwiththat/message

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2023.03.07.531472v1?rss=1 Authors: Frolikova, M., Blazikova, M., Capek, M., Chmelova, H., Valecka, J., Kolackova, V., Valaskova, E., Gregor, M., Komrskova, K., Horvath, O., Novotny, I. Abstract: Super-resolution (SR) microscopy is a cutting-edge method that can provide detailed structural information with high resolution. However, the thickness of the specimen has been a major limitation for SR methods, and larger structures have posed a challenge. To overcome this, the key step is to optimize sample preparation to ensure optical homogeneity and clarity, which can enhance the capabilities of SR methods for the acquisition of thicker structures. Oocytes are the largest cells in the mammalian body and are crucial objects in reproductive biology. They are especially useful for studying membrane proteins. However, oocytes are extremely fragile and sensitive to mechanical manipulation and osmotic shocks, making sample preparation a critical and challenging step. We present an innovative, simple, and sensitive approach to oocyte sample preparation for 3D STED acquisition. This involves alcohol dehydration and mounting into a high refractive index medium. This extended preparation procedure allowed us to successfully obtain a unique 2-channel 3D STED super-resolution image of an entire mouse oocyte. By optimizing sample preparation, we can overcome the limitations of SR methods and obtain high-resolution images of larger structures, such as oocytes, Knowledge of which are important for understanding fundamental biological processes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the impact of genetics lab on euploid and live birth rates (01:58), pregnancy & neonatal outcomes after long-term vitrification (09:44), risk of hypertensive disorders of pregnancy in donated-embryos pregnancies (18:08), diagnosing ectopic pregnancy using Bayes theorem (25:42), markers of ovarian reserve as predictors of future fertility (34:36) and letrozole-induced ovulation in women with PCOS and letrozole resistance (40:46). View the January 2023 issue of Fertility and Sterility: Volume 119, No 1 - https://www.fertstert.org/issue/S0015-0282(22)X0004-6  View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peak at this month's Fertility & Sterility! Topics this month include outcomes after intrauterine insemination in low AMH versus normal AMH women (2:57), impact of low initial serum hCGs after IVF pregnancies on pregnancy complications and placental pathology (18:23), single embryo transfer of good quality embryo versus double embryo transfer with 2nd poor-quality embryo (29:00), cardiometabolic health outcomes in fresh vs frozen embryo transfers (41:15), pathways of mTOR in primary ovarian insufficiency (52:36) and a review of surgery for endometriomas (56:58) View the December 2022 issue of Fertility and Sterility: Volume 118, No 6 - https://www.fertstert.org/issue/S0015-0282(21)X0026-X View Fertility and Sterility at https://www.fertstert.org/

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2022.12.06.519314v1?rss=1 Authors: Briley, S. M., Ahmed, A. A., Jiang, P., Hartig, S. M., Schindler, K., Pangas, S. Abstract: In mammals, meiotically competent oocytes develop cyclically during ovarian folliculogenesis. During folliculogenesis, prophase I arrested oocytes are transcriptionally active, producing and storing transcripts required for their growth and for early stages of embryogenesis prior to the maternal to zygotic transition. Defective oocyte development during folliculogenesis leads to meiotic defects, aneuploidy, follicular atresia, or non-viable embryos. Here we generated a novel oocyte-specific knockout of the SUMO E2 ligase, Ube2i, using Zp3-cre to test its function during folliculogenesis. Ube2i Zp3-cre+ female mice are sterile with oocytes that arrest in meiosis I with defective spindles and chromosome alignment. Fully grown mutant oocytes abnormally maintain transcription but downregulate maternal effect genes and prematurely activate the zygotic transcriptional program. Thus, this work uncovers UBE2i as a novel orchestrator of chromatin and transcriptional regulation in mouse oocytes. Copy rights belong to original authors. Visit the link for more info Podcast created by Paper Player, LLC

Fertility & Sterility brings you some great interviews from ASRM 2022 in Anaheim, CA! In Part 1, we discuss science with leaders in the field and prefesenters from the conference. Topics this month include career-focused women and family planning (Jen Bakkenson) (3:20), live imaging of embryo development (Nicholas Plachta) (8:02), predicting outcomes using AMH in fertilty preservation (Cassandra Roeca) (19:15), IVF stimulation outcomes by race and ethnicity (Iris Lee) (21:09), a chat with Dr. Beth Rakowsky (30:53), assessing expansion of fertility preservation coverage (Allison Komorowski) (35:47), a discussion on uterine NK cells in reproduction (Jessica Kanter) (39:56), the potential of mitochondrial enhacement (Richard Scott) (44:15), updates in recurrent implantation failure (Paul Pirtea, Dominique De Ziegler) (49:43) and a discussion about male infertility (Luis Hoyos, Thomas Masterson) (1:00:18). View Fertility and Sterility at https://www.fertstert.org/

Fertility & Sterility brings you some great interviews from ASRM 2022 in Anaheim, CA! In Part 2, we discuss how the people of ASRM are impacting the field and the community. Topics include advocacy for reproductive rights (Serena Chen, Natalie Crawford, Sean Tipton) (00:41), the future of ASRM with Dr. Michael Thomas (14:52), a look at the a pilot ASRM Pipeline Mentoring Program (22:36), a chat about F&S Reviews (35:17), a look at the Stanford REI Physician Survey (39:36), a look at venture capital in REI with Dr. David Sable (43:07), a chat about F&S Reports (47:09), a physician's path to industry with Dr. Vrunda Desai (53:03), the SRS Surgical Scholars Program (1:00:51), a chat with the SART Podcast team (1:06:13), a chat about F&S Science (1:10:04), and a look at the Reproductive Health Justice Summit (1:13:41). View Fertility and Sterility at https://www.fertstert.org/

Sam is out of town and his girlfriend, Mandy, is starting to feel the pressure. She asks her friend Rajat to come over and help out with the household chores, but Rajat has other plans.

Take a sneak peak at this month's Fertility & Sterility! Topics this month include longitudinal semen assesment and live birth (2:20), placenta previas in ART pregnancies (11:35), cost-effectiveness for cryopreservation based on SART data (19:12), systematic review of subsequent pregnancy outcomes in couples with parental abnormal chromosomal karyotypes & recurrent pregnancy loss (28:42), prevelance of endometrioma in deep infiltrative endometriosis (39:40) and perinatal outcomes of singleton and twin pregnancies using donor versus partner sperm (45:01). View Fertility and Sterility Volume 118 Issue 5 - https://www.fertstert.org/issue/S0015-0282(21)X0025-8 View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peak at this month's Fertility & Sterility! Topics this month include the natural history of fibroids in pregnancy (1:54), semen quality in sons of subfertile couples (09:33), oocyte maturity ratio and live birth rate (18:41), pregnancy outcomes after frozen transfer using Letrozole (22:28), endometrial receptivity assay after failed transfer attempt (36:08), colorectal cancer risk after use of fertility drugs (45:08), and cesarean-section scar defects and abnormal uteirne bleeding (56:13). View Fertility and Sterility Volume 118 Issue 4 - https://www.fertstert.org/issue/S0015-0282(21)X0024-6 View Fertility and Sterility at https://www.fertstert.org/

How can we improve managing a poor ovarian response in the patient with diminished ovarian reserve? This Journal Club Global discusses the April 2022 Views and Reviews Series in F&S, in conjuction with the faculty and fellows of Boston IVF and UCSF.

Take a sneak peak at this month's Fertility & Sterility! Topics this month include ICSI vs IVF for non-male factor infertility (01:42), geographic variations of semen parameters (10:18), euploid embryos and chance of subsequent live birth (19:38), DOR diagnosis and aneuploidy and live birth rate (31:06), interpregnancy intervals in ART pregnancies (38:15) and uterine transplantation outcomes (45:32). View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peak at this month's Fertility & Sterility! Topics this month include a Fertile Battle on optimal endometrial lining in ART (2:00), an RCT using FSH with a trigger (8:40), the relationship between paternal factors and embryonic aneuploidy of paternal origin (20:00), an RCT looking at live birth using the endometrial receptivity analysis (27:35), risk of miscarriage after treatment for chronic endometritis and IVF/ICSI (41:25), Cardiometabolic risks in middle childhood (47:00) and evaluating the efficacy of different treatments for c-section scar ectopics (51:40). View the August 2022 issue of Fertility and Sterility, Volume 118, No. 2 at https://www.fertstert.org/issue/S0015-0282(21)X0022-2 View Fertility and Sterility at https://www.fertstert.org/

Take a sneak peak at this month's Fertility & Sterility! Topics this month include a cost-effectiveness analysis of PGT-M vs prenatal diagnosis for Huntington disease (3:20), a machine learning model for predicting the optimal day of trigger (13:00), a systematic review and meta-analysis of Vitamin D and miscarriage (26:13), AMH and leukocyte aging markers (32:20), 15 years of autologous oocyte thaw outcomes from one academic fertility center (43:20) and look at the relation between smoking and infertility (50:56). View the July 2022 issue of Fertility and Sterility, Volume 118, No. 1 at https://www.fertstert.org/issue/S0015-0282(21)X0021-0 View Fertility and Sterility at https://www.fertstert.org/

For the third episode on the topic of vitrification, Adjunct Professor Laura Rienzi, PhD and Professor William Ledger discuss how and when to communicate with patients and the importance of managing their expectations. See acast.com/privacy for privacy and opt-out information.

Our host Steve Levett, PhD is joined by experts Dr Daniela Nogueira, PhD and Arianna D'Angelo, MD to talk about how crucial communication with the clinical team is after oocyte pick-up. See acast.com/privacy for privacy and opt-out information.

Our host Steve Levett, PhD is joined by experts Dr Daniela Nogueira, PhD and Arianna D'Angelo, MD to discuss the importance of laboratory standards and recommendations for optimising oocyte pick-up. See acast.com/privacy for privacy and opt-out information.

Our host Steve Levett, PhD is joined by Arianna D'Angelo, MD, Clinical Lead Reproductive Medicine and Honorary Senior Lecturer at Wales Fertility Institute and Cardiff University. In this insightful episode they discuss how your patients can best prepare for oocyte pick up. See acast.com/privacy for privacy and opt-out information.

It is Fertility Preservation Month, and today on the show we are speaking with Dr. Sigal Klipstein about planned oocyte cryopreservation. More information on these topics is at www.asrm.org Tell us your thoughts on the show by e-mailing asrm@asrm.org Please subscribe and rate the show on Apple podcasts, Google Play, or wherever you get your podcasts. ASRM Today Series Podcasts are supported in part by the ASRM Corporate Member Council.

In the second of a two-part series from ASRM 2021 Scientific Congress and Expo, we bring you interviews and discussion about the groundbreaking science being presented at the conference. Interviews in Part 2 include: Dr. Samantha Pfeifer (1:12), Dr. Nicole Doyle (12:53), Dr. Jerrine Morris (20:43), Dr. Yigit Cakiroglu (30:30), Dr. Jonah Bardos (38:42), Dr. Jen Bakkensen (47:48), Dr. Alexandra G. Huttler (56:16), Dr. Lauren Butler (1:00:40) and Dr. Kara Goldman (1:05:33). View Fertility and Sterility at https://www.fertstert.org/ More information about ASRM 2021 can be found at https://asrmcongress.org 

Take a sneak peak at this month's Fertility and Sterility! Topics include transgender reproductive care (01:35), surgical management of intramural fibroids (08:19), TESE for nonazoospermic male factor infertility (15:26), COVID-19 screening and cycle outcomes (19:02), ectopic pregnancy biomarkers (27:37), ambient temperature and ovarian reserve (36:38), national oocyte thaw outcomes (45:16) and using AMH to determine PCOM (52:01). View Fertility and Sterility at https://www.fertstert.org/

In episode five of Fertility Insights, Steven Fleming, PhD, continues the conversation about IVM for fertility preservation in cancer patients with experts Michel De Vos, PhD and Robert Gilchirst, PhD.Robert talks to Steven about considering the ovarian medulla as a valuable source of oocytes for recovery and how best to achieve maximum developmental potential of oocytes. Michel discusses how to manage patients presenting with ovarian tumours to give them the best chance of fertility preservation.Look out for the final episode on this topic! See acast.com/privacy for privacy and opt-out information.

We take control of our ticking clock by giving you the 101 on egg freezing. We kickoff our special series with Dr. Aubrey Señeris of Konsulta.md and a friend of ours, Tisha, who is single and freezing her eggs this 2021.

Take a sneak peak at this month's Fertility and Sterility! Topics include new insights in male sterilization, obesity and risk of a euploid embryo miscarriage, endometrial preparation in frozen embryo transfers, as well as a look into three new Practice Committee documents for infertility providers. View Fertility and Sterility June 2021 Volume 115 Issue 6 View Fertility and Sterility at https://www.fertstert.org/

How does egg freezing reshape our conception of time, aging and fertility? In her new monograph, Freezing Fertility: Oocyte Cryopreservation and the Gender Politics of Aging (NYU Press, 2020) Dr. Lucy van de Wiel explores the significance of egg freezing in re-orienting the temporality of the gender politics of aging. Dr. van de Wiel argues that it is critical to examine the politicized dimensions of egg freezing because it transforms the broader discourses around aging and normative timeline of women's reproduction even though the technology is only accessible for an elite few.  Through a cultural analysis of popular media and documentaries to highlight the role of rhetoric in creating conditions that motivate women in making decisions about their reproductive futures, Dr. van de Wiel criticizes the moralistic boundaries between social and medical utilized by some state actors to condemn women who decide to freeze their eggs for their careers. Dr. van de Wiel also highlights the role of financialized capitalism in refiguring fertility under the investment logic of optimization to speculate on the potential risks posed by anticipated infertility. Egg freezing technology shows us how the biopolitical control of population shifted to managing fertility, which transcends national borders as frozen eggs circulate transnationally for assisted reproduction as well as stem cell research. An incredibly rich and nuanced book, Freezing Fertility would be an invaluable read for anyone who is interested in the politics of reproduction, ART, gendered politics of aging, mortality, life, and regeneration.   Dr. Lucy van de Wiel is a Research Associate at the Reproductive Sociology Research Group (ReproSoc), University of Cambridge. Her research focuses on the social and cultural analysis of assisted reproductive technologies like egg freezing, time-lapse embryo selection and cross-border reproductive care. Da In Ann Choi is a PhD student at UCLA in the Gender Studies department. Her research interests include care labor and migration, reproductive justice, social movement, citizenship theory, and critical empire studies. She can be reached at dainachoi@g.ucla.edu. Learn more about your ad choices. Visit megaphone.fm/adchoices Support our show by becoming a premium member! https://newbooksnetwork.supportingcast.fm/gender-studies

How does egg freezing reshape our conception of time, aging and fertility? In her new monograph, Freezing Fertility: Oocyte Cryopreservation and the Gender Politics of Aging (NYU Press, 2020) Dr. Lucy van de Wiel explores the significance of egg freezing in re-orienting the temporality of the gender politics of aging. Dr. van de Wiel argues that it is critical to examine the politicized dimensions of egg freezing because it transforms the broader discourses around aging and normative timeline of women's reproduction even though the technology is only accessible for an elite few.  Through a cultural analysis of popular media and documentaries to highlight the role of rhetoric in creating conditions that motivate women in making decisions about their reproductive futures, Dr. van de Wiel criticizes the moralistic boundaries between social and medical utilized by some state actors to condemn women who decide to freeze their eggs for their careers. Dr. van de Wiel also highlights the role of financialized capitalism in refiguring fertility under the investment logic of optimization to speculate on the potential risks posed by anticipated infertility. Egg freezing technology shows us how the biopolitical control of population shifted to managing fertility, which transcends national borders as frozen eggs circulate transnationally for assisted reproduction as well as stem cell research. An incredibly rich and nuanced book, Freezing Fertility would be an invaluable read for anyone who is interested in the politics of reproduction, ART, gendered politics of aging, mortality, life, and regeneration.   Dr. Lucy van de Wiel is a Research Associate at the Reproductive Sociology Research Group (ReproSoc), University of Cambridge. Her research focuses on the social and cultural analysis of assisted reproductive technologies like egg freezing, time-lapse embryo selection and cross-border reproductive care. Da In Ann Choi is a PhD student at UCLA in the Gender Studies department. Her research interests include care labor and migration, reproductive justice, social movement, citizenship theory, and critical empire studies. She can be reached at dainachoi@g.ucla.edu. Learn more about your ad choices. Visit megaphone.fm/adchoices Support our show by becoming a premium member! https://newbooksnetwork.supportingcast.fm/communications

How does egg freezing reshape our conception of time, aging and fertility? In her new monograph, Freezing Fertility: Oocyte Cryopreservation and the Gender Politics of Aging (NYU Press, 2020) Dr. Lucy van de Wiel explores the significance of egg freezing in re-orienting the temporality of the gender politics of aging. Dr. van de Wiel argues that it is critical to examine the politicized dimensions of egg freezing because it transforms the broader discourses around aging and normative timeline of women's reproduction even though the technology is only accessible for an elite few.  Through a cultural analysis of popular media and documentaries to highlight the role of rhetoric in creating conditions that motivate women in making decisions about their reproductive futures, Dr. van de Wiel criticizes the moralistic boundaries between social and medical utilized by some state actors to condemn women who decide to freeze their eggs for their careers. Dr. van de Wiel also highlights the role of financialized capitalism in refiguring fertility under the investment logic of optimization to speculate on the potential risks posed by anticipated infertility. Egg freezing technology shows us how the biopolitical control of population shifted to managing fertility, which transcends national borders as frozen eggs circulate transnationally for assisted reproduction as well as stem cell research. An incredibly rich and nuanced book, Freezing Fertility would be an invaluable read for anyone who is interested in the politics of reproduction, ART, gendered politics of aging, mortality, life, and regeneration.   Dr. Lucy van de Wiel is a Research Associate at the Reproductive Sociology Research Group (ReproSoc), University of Cambridge. Her research focuses on the social and cultural analysis of assisted reproductive technologies like egg freezing, time-lapse embryo selection and cross-border reproductive care. Da In Ann Choi is a PhD student at UCLA in the Gender Studies department. Her research interests include care labor and migration, reproductive justice, social movement, citizenship theory, and critical empire studies. She can be reached at dainachoi@g.ucla.edu. Learn more about your ad choices. Visit megaphone.fm/adchoices Support our show by becoming a premium member! https://newbooksnetwork.supportingcast.fm/science

How does egg freezing reshape our conception of time, aging and fertility? In her new monograph, Freezing Fertility: Oocyte Cryopreservation and the Gender Politics of Aging (NYU Press, 2020) Dr. Lucy van de Wiel explores the significance of egg freezing in re-orienting the temporality of the gender politics of aging. Dr. van de Wiel argues that it is critical to examine the politicized dimensions of egg freezing because it transforms the broader discourses around aging and normative timeline of women's reproduction even though the technology is only accessible for an elite few.  Through a cultural analysis of popular media and documentaries to highlight the role of rhetoric in creating conditions that motivate women in making decisions about their reproductive futures, Dr. van de Wiel criticizes the moralistic boundaries between social and medical utilized by some state actors to condemn women who decide to freeze their eggs for their careers. Dr. van de Wiel also highlights the role of financialized capitalism in refiguring fertility under the investment logic of optimization to speculate on the potential risks posed by anticipated infertility. Egg freezing technology shows us how the biopolitical control of population shifted to managing fertility, which transcends national borders as frozen eggs circulate transnationally for assisted reproduction as well as stem cell research. An incredibly rich and nuanced book, Freezing Fertility would be an invaluable read for anyone who is interested in the politics of reproduction, ART, gendered politics of aging, mortality, life, and regeneration.   Dr. Lucy van de Wiel is a Research Associate at the Reproductive Sociology Research Group (ReproSoc), University of Cambridge. Her research focuses on the social and cultural analysis of assisted reproductive technologies like egg freezing, time-lapse embryo selection and cross-border reproductive care. Da In Ann Choi is a PhD student at UCLA in the Gender Studies department. Her research interests include care labor and migration, reproductive justice, social movement, citizenship theory, and critical empire studies. She can be reached at dainachoi@g.ucla.edu. Learn more about your ad choices. Visit megaphone.fm/adchoices Support our show by becoming a premium member! https://newbooksnetwork.supportingcast.fm/new-books-network

How does egg freezing reshape our conception of time, aging and fertility? In her new monograph, Freezing Fertility: Oocyte Cryopreservation and the Gender Politics of Aging (NYU Press, 2020) Dr. Lucy van de Wiel explores the significance of egg freezing in re-orienting the temporality of the gender politics of aging. Dr. van de Wiel argues that it is critical to examine the politicized dimensions of egg freezing because it transforms the broader discourses around aging and normative timeline of women's reproduction even though the technology is only accessible for an elite few.  Through a cultural analysis of popular media and documentaries to highlight the role of rhetoric in creating conditions that motivate women in making decisions about their reproductive futures, Dr. van de Wiel criticizes the moralistic boundaries between social and medical utilized by some state actors to condemn women who decide to freeze their eggs for their careers. Dr. van de Wiel also highlights the role of financialized capitalism in refiguring fertility under the investment logic of optimization to speculate on the potential risks posed by anticipated infertility. Egg freezing technology shows us how the biopolitical control of population shifted to managing fertility, which transcends national borders as frozen eggs circulate transnationally for assisted reproduction as well as stem cell research. An incredibly rich and nuanced book, Freezing Fertility would be an invaluable read for anyone who is interested in the politics of reproduction, ART, gendered politics of aging, mortality, life, and regeneration.   Dr. Lucy van de Wiel is a Research Associate at the Reproductive Sociology Research Group (ReproSoc), University of Cambridge. Her research focuses on the social and cultural analysis of assisted reproductive technologies like egg freezing, time-lapse embryo selection and cross-border reproductive care. Da In Ann Choi is a PhD student at UCLA in the Gender Studies department. Her research interests include care labor and migration, reproductive justice, social movement, citizenship theory, and critical empire studies. She can be reached at dainachoi@g.ucla.edu. Learn more about your ad choices. Visit megaphone.fm/adchoices Support our show by becoming a premium member! https://newbooksnetwork.supportingcast.fm/science-technology-and-society

How does egg freezing reshape our conception of time, aging and fertility? In her new monograph, Freezing Fertility: Oocyte Cryopreservation and the Gender Politics of Aging (NYU Press, 2020) Dr. Lucy van de Wiel explores the significance of egg freezing in re-orienting the temporality of the gender politics of aging. Dr. van de Wiel argues that it is critical to examine the politicized dimensions of egg freezing because it transforms the broader discourses around aging and normative timeline of women's reproduction even though the technology is only accessible for an elite few.  Through a cultural analysis of popular media and documentaries to highlight the role of rhetoric in creating conditions that motivate women in making decisions about their reproductive futures, Dr. van de Wiel criticizes the moralistic boundaries between social and medical utilized by some state actors to condemn women who decide to freeze their eggs for their careers. Dr. van de Wiel also highlights the role of financialized capitalism in refiguring fertility under the investment logic of optimization to speculate on the potential risks posed by anticipated infertility. Egg freezing technology shows us how the biopolitical control of population shifted to managing fertility, which transcends national borders as frozen eggs circulate transnationally for assisted reproduction as well as stem cell research. An incredibly rich and nuanced book, Freezing Fertility would be an invaluable read for anyone who is interested in the politics of reproduction, ART, gendered politics of aging, mortality, life, and regeneration.   Dr. Lucy van de Wiel is a Research Associate at the Reproductive Sociology Research Group (ReproSoc), University of Cambridge. Her research focuses on the social and cultural analysis of assisted reproductive technologies like egg freezing, time-lapse embryo selection and cross-border reproductive care. Da In Ann Choi is a PhD student at UCLA in the Gender Studies department. Her research interests include care labor and migration, reproductive justice, social movement, citizenship theory, and critical empire studies. She can be reached at dainachoi@g.ucla.edu. Learn more about your ad choices. Visit megaphone.fm/adchoices Support our show by becoming a premium member! https://newbooksnetwork.supportingcast.fm/medicine

We answered your questions about eggs including:Oocyte, when are how are they made?Can we influence egg quality?What is egg quality?Can we increase AMH?Do Antibiotics effect egg quality?What is the role of supplements and eggs?How does endometriosis effect eggs?You might like these episodes of Knocked Up:AMH, the fertility test?Professor David Gardner on the science behind the best IVF success ratesNutrition and Fertility With Dietitian Wendy FedeleThis episode is brought to you by Lovers - Individualised intimate care created by intimate health authority, Gynaecologist and Reproductive Endocrinologist Dr Raelia Lew.Find us on Instagram - @knockeduppodcastJoin our community! Follow Women's Health Melbourne on Facebook and Instagram (@womenshealthmelbourne), and follow Dr Raelia Lew on Instagram (@drraelialew).Have a question about women's health? Is there a specific topic you'd like us to cover? Email podcast@womenshealthmelbourne.com.au. We'll keep all requests anonymous.Women's Health Melbourne is a holistic care precinct, for more information about the work we do click here.Hosts: Dr Raelia Lew and Jordi Morrison

a16z general Vineeta Agarwala, MD Ph.D, and deal partners Judy Savitskaya, Ph.D and Justin Larkin, MD join host Lauren Richardson, Ph.D to discuss the results and implications of the article "Reconstitution of the oocyte transcriptional network with transcription factors" by Nobuhiko Hamazaki, Hirohisa Kyogoku, Hiromitsu Araki, Fumihito Miura, Chisako Horikawa, Norio Hamada, So Shimamoto, Orie Hikabe, Kinichi Nakashima, Tomoya S. Kitajima, Takashi Ito, Harry G. Leitch and Katsuhiko Hayashi, published in Nature.

Oocyte Cryopreservation Latest Updates --- Send in a voice message: https://anchor.fm/dr-richa-sharma/message

In this episode, we have Dr. Jennifer Mersereau and Dr. Suleena Kaira on the show to discuss the update document on Oocyte Cryopreservation.   For more information on this topic go to http://www.asrm.org

Dr. Julia Kim, from RMA's REI fellowship program, reviews the effects of obesity on fertility, as well as the impact of being overweight on infertility treatments such as in vitro fertilization (IVF). She also shares the results of her recent ABC Trial, a study aimed at identifying whether male or female obesity affects gametogenesis and reproductive outcomes.   Podcast website: https://www.ivi-rmainnovation.com/?utm_source=podcast&utm_medium=episode&utm_campaign=ep11

Getting pregnant can be a dream for couple planning to get pregnant and the egg freezing or oocyte can prove to be miracle and help complete family even at older age.

How do you need to prepare for egg pick up day or oocyte Retrieval process during the IVF Treatment process?

Link to bioRxiv paper: http://biorxiv.org/cgi/content/short/2020.07.30.228270v1?rss=1 Authors: Imakubo, M., Takayama, J., Okada, H., Onami, S. Abstract: Background: Oocyte quality decreases with aging, thereby increasing errors in fertilization, chromosome segregation, and embryonic cleavage. Oocyte appearance also changes with aging, suggesting a functional relationship between oocyte quality and appearance. However, no methods are available to objectively quantify age-associated changes in oocyte appearance. Results: We show that statistical image processing of Nomarski differential interference contrast microscopy images can be used to quantify age-associated changes in Caenorhabditis elegans oocyte appearance. Max-min Value (mean difference between the maximum and minimum intensities within each moving window) quantitatively characterized the difference in oocyte cytoplasmic texture between 1- and 3-day-old adults (Day 1 and Day 3 oocytes, respectively). With an appropriate parameter set, the gray level co-occurrence matrix (GLCM)-based texture feature Correlation (COR) more sensitively characterized this difference than the Max-min Value. Manipulating the smoothness of and/or adding irregular structures to the cytoplasmic texture of Day 1 oocyte images reproduced the difference in Max-min Value but not in COR between Day 1 and Day 3 oocytes. Increasing the size of granules in synthetic images recapitulated the age-associated changes in COR. Manual measurements validated that the cytoplasmic granules in oocytes become larger with aging. Conclusions: The Max-min Value and COR objectively quantify age-related changes in C. elegans oocyte in Nomarski DIC microscopy images. Our methods provide new opportunities for understanding the mechanism underlying oocyte aging. Copy rights belong to original authors. Visit the link for more info

Have you ever thought about freezing your eggs? If you're a woman in your thirties, heck, if you're a woman in your twenties, chances are egg freezing has come across your radar at least once. So much around it can feel overwhelming, scary and unknown. How does egg freezing actually work?? How much does it cost? Does it hurt? How old should you be when you freeze your eggs? What are the success rates? How does it affect future fertility?   In this episode - the very first episode of In Her Prime, Talia sits down with Dr. Dan Nayot to learn all about social egg freezing. From the testing that's done, to the hormones you take, to the egg freezing process itself, a lot is explained. For reference, Dr. Nayot practices out of Toronto, Canada so the information provided on things like cost and insurance is based on Canadian stats. That being said, this episode is still immensely relevant to anyone, anywhere who wants to learn more about egg freezing as the process itself remains largely the same wherever egg freezing is done.     The purpose of this episode is to help provide you with more in-depth information on social egg freezing from a medical expert, not to make recommendations on what you should or should not do. Of course, that decision belongs to you. Listen and enjoy, and if you have any questions, comments, or personal experiences you'd like to share, you can do so in our free, private Facebook community, PrimeMates. Sign up here to join the conversation.   Dr. Dan Nayot, MD, M.Sc., FRCSC, REI Reproductive Endocrinology & Infertility Specialist (REI) Dr. Dan Nayot trained at internationally recognized institutions, obtaining his medical degree from the University of Western Ontario, and completing his Obstetrics and Gynecology Residency at the University of Toronto. Shortly thereafter, Dr. Nayot completed a Royal College fellowship in Gynecologic Reproductive Endocrinology and Infertility at McGill University, Canada's largest University-based reproductive centre. Continuing his academic pursuits at Harvard University, Dr. Nayot received his Masters of Science degree in Clinical Epidemiology. He has been awarded several research grants and academic scholarships, authored numerous scientific papers, and presented at international medical conferences. Dr. Nayot is an active reviewer for several esteemed fertility scientific journals and continues to be involved in clinical research. He is an Assistant Professor at the University of Toronto in the Department of Obstetrics and Gynecology, and trains students at various stages of their medical programs. Dr. Nayot is committed to providing his patients with individualized evidence-based care. He is a strong believer in empowering patients to make educated decisions, whether by appraising the latest research studies or facilitating a holistic team approach to infertility

Oocyte cryopreservation, or egg freezing, is a viable option for people with ovaries who want to extend their fertility or begin IVF. While it’s becoming more commonplace, the experience is not as simple as it’s sometimes made out to be: hormonal self-injections, frequent appointments, and going under anesthesia are all part of the process. So what’s really involved, and what can you do to make the procedure as smooth as possible? Sophie shares her tips on today’s episode.

Dr Lynn Westphal is CMO at Kindbody, a new model for fertility health care.( They have mobile clinics (the fertility buses) positioned in high traffic areas so women can potp in for a chat and organise a fertility assessment. Their primary group of patients is women seeking to freeze their oocytes, however they offer IVF cycles and a full range of fertility services. Lynn set up one of the first cyropreservation programs for oncology patients back in 1998 at Stanford University. With such an incredible wealth of fertility experience, the conversation with Lynn was enlightening. https://kindbody.com/    

We have a very special guest with us today! Matt Doran bravely shares with us his very personal story of growing up and finding out that he was a ‘donor’ conceived human being. Matt is both an aerospace mechanical engineer as well as an advocate against donor conception. Matt created and founded the first free worldwide social network for ‘donor’ conceived people, sperm/egg/embryo/surrogate ‘donors’, and social parents of ‘donor’ conceived people. His mission is to help connect biological families and offer support to other ‘donor’ conceived people so that they can create a demographic body that will create legislative change. You can find more about his foundation at DonorChildren.com.

Kellie Harriden shares her story, 8 very long years of fertility treatment. Kellie underwent numerous IVF cycles which failed, she describes her feelings and thoughts as she went through all the decisions that ultimately led her to India. In India, Kellie used a surrogate to achieve her dream of having a baby, during the process there were many hopes, fears and feelings of despair. Ultimately, Kellie and her partner Ian, finally came back to Australia with twins Bane and Daya. Her story is told in the book, Long-Awaited Child, a brilliant read and one that gives you a fantastic insight into the emotional mindset when traveling the pathway to parenthood! Website ~ www.kellieharriden.com Email ~ info@longawaitedchild.com

Procrastination - the action of delaying or postponing something! Is this one of your bad habits? How can you get rid of it? How does it impact your fertility treatment? Join me for a discussion about this annoying habit. Let's Talk IVF is a podcast series for everyone starting or undergoing fertility treatment. We will discuss the Science, the Emotions and Everything in between. You can contact me directly through my website/Facebook/Instagram pages. Thank you for joining me and I look forward to hearing from you.   Book for your free Clarity Call  http:www.ivfcoachingclinic.as.me Visit our Website http://ivfcoachingclinic.com.au/ Follow us on Facebook https://www.facebook.com/ivfcoachingclinic/ Follow us on Instagram https://www.instagram.com/ivfcoachingclinic/ Follow us on LinkedIn www.linkedin.com/in/sophia-baseotto-5870395/

In this podcast, Dr. Mark Damario, a Reproductive Endocrinologist and Infertility Specialist with the Center for Reproductive Medicine, presented at Ridgeview Medical Center's Live Friday CME Series on May 24, 2019. At this event, Dr. Damario provided insights to the emerging roles of assisted reproductive technologies. Enjoy the podcast! Objectives:    Upon completion of this podcast, participants should be able to: Describe the role of assisted reproductive technologies for infertile and non-infertile conditions. Differentiate the various forms of assisted reproductive technologies. Identify changes and new developments in assisted reproductive technologies. CME credit is only offered to Ridgeview Providers for this podcast activity. Complete and submit the online evaluation form, after viewing the activity.  Upon successful completion of the evaluation, you will be e-mailed a certificate of completion within 2 weeks.  You may contact the accredited provider with questions regarding this program at  rmccredentialing@ridgeviewmedical.org. Click on the following link for your CME credit: CME Evaluation: "Update in Assisted Reproductive Technologies" (**If you are listening to the podcasts through iTunes on your laptop or desktop, it is not possible to link directly with the CME Evaluation for unclear reasons. We are trying to remedy this. You can, however, link to the survey through the Podcasts app on your Apple and other smart devices, as well as through Spotify, Stitcher and other podcast directory apps and on your computer browser at these websites. We apologize for the inconvenience.)  The information provided through this and all Ridgeview podcasts as well as any and all accompanying files, images, videos and documents is/are for CME/CE and other institutional learning and communication purposes only and is/are not meant to substitute for the independent medical judgment of a physician, healthcare provider or other healthcare personnel relative to diagnostic and treatment options of a specific patient's medical condition.”   FACULTY DISCLOSURE ANNOUNCEMENT  It is our intent that any potential conflict should be identified openly so that the listeners may form their own judgments about the presentation with the full disclosure of the facts. It is not assumed any potential conflicts will have an adverse impact on these presentations. It remains for the audience to determine whether the speaker’s outside interest may reflect a possible bias, either the exposition or the conclusions presented. Planning committee members and presenter(s) have disclosed they have no significant financial relationship with a pharmaceutical company and have disclosed that no conflict of interest exists with the presentation/educational event. SHOW NOTES:  INTRODUCTION: Dr. Damario is the medical director of the Center for Reproductive Medicine in St. Paul, MN; and is a practicing reproductive medicine specialist who earned his medical degree at the Medical College of Virginia, and completed his OB/GYN residency there as well. He then went on to complete fellowships in reproductive surgery at Emory University and reproductive endocrinology at Cornell. We were delighted to have him join us on May 24, 2019, to provide his insights and updates on assisted reproductive technology. CHAPTER 1: ART (assisted reproductive technology) was initially done in rabbit models, and then the first successful IVF birth in humans in 1978. Use of oocyte donation in 1983, a surrogate mother in 1986. Pre-implantation genetic dx in an embryo in 1989, and a single sperm injected in an egg in 1992. These were major breakthroughs. Where is ART these days? There are over 500 clinics in the U.S. that contribute for the most part to the national database on stats for ART. Ultimately, there are over 65,000 live births, and the percentage is now about 1.9% of total babies born now in the U.S.   Forbes.com in 2012, estimated 900,000 approximately conceived persons in the U.S. derived from IVF. That's about 305 people at Times Square bumping into each other on any given day. In 2017, the trend of multiple pregnancies in IVF and ART has come down. This issue has been contentious in the field of ART. Triplets in particular happen less often. Less than 1% from 7% in the mid-1990s. Twins have decreased in rate as well. Oocyte cryopreservation has continued to increase, as it is much more efficient now for donor and fertility preservation. More elective single embryo transfers as well. Indications for IVF include: Tubal factors such as occluded or absent tubes are indications, Male factors, such as severe oligospermia is also an indication for IVF. Advanced endometriosis and failed induction of ovulation, diminished ovarian reserve, unexplained infertility and a variety of specific conditions, such as the need for pre-implantation genetic diagnosis are also indications. Dr. Damario's program treats women age less than 45 with their own eggs and less than 50 with donor eggs. Smoking is a contraindication. BMI is also an issue for patients. Assessment of the woman will include antimullerian hormone (which has key roles in folliculogenesis), basal antra follicle count by U/S (an ovarian follicle in the latter stage of formation) and less often day 3 FSH and estradiol. Sonohysterography or saline hysterography, semenalysis, and sperm penetration testing, cervical canal evaluation, testing for Hep B and C, HIV and Syphilis are also performed ahead of time. How does the treatment phase of IVF work? Pituitary inhibition needs to happen in order to suppress the LH surge before oocyte retrieval. OCPs are given ahead of time to suppress other ovarian cysts, and to help start some of the later fertility medications. Pituitary suppression with Lupron or antagonists, such as Ganorelix. The gonadotropins are given after withdrawal bleeding from discontinuation of the pill and for about 8 to 12 days. Ultrasounds for follicles and estradiol accounts are performed. HCG is then given if all looks well with the above steps. It is similar to LH and then oocyte retrieval is scheduled 36 hours later. This is a precise action so as to not have egg immaturity or have ovulation occur. Embryo transfer then happens on the 5th day, most commonly. Usually then a pregnancy test happens on the 9th day. egg retrieval occurs transvaginally with ultrasound guidance. This is a day surgery procedure, and is generally tolerated well. Dr. Damario shows a short video of the procedure at this time. The embryologist takes the follicular fluid to ID eggs. They are graded for maturity, and they simultaneously prepare sperm. Eggs are incubated, then are exposed to sperm. They are observed the following day, embryos cleave and then on days 3 to 5, the embryo is transferred. A mature egg is actually the largest cell in the human body, and is surrounded by a clump of cells called a corona. Micro-droplet of sperm onto the egg and intracytoplasmic injection of a single sperm injected into the egg can also be done, especially if sperm quality is lower. The embryo is then cultured and grown, with cell division occurring daily and exponentially. A morula, which is many cells, is followed by a blastocyst forming, which is an expansion with an inner cell mass and fluid space. At this stage, the embryo is felt to be doing well. If they're not doing well, the embryo won't be chosen for transfer. Egg fertilization occurs at a rate of about 75%. The embryo is transferred into the uterus with transabdominal U/S guidance as it is placed into the uterus. Little to no anesthesia is required for this. CHAPTER 2: Outcomes are impacted by age to a fair degree, obviously higher (success) in younger women. The older the patient the less likely it will take effect. Cryogenically preserved embryos are doing well in transfer, in fact slightly better in some cases than traditional transfer. Risks of IVF include: multiple pregnancies, ovarian hyperstimulation syndrome, bleeding and infection, which are rare. Adverse pregnancies such as ectopic and miscarriage can occur. There is a possibility of subtle increase in congenital anomalies. Third party reproduction options include: oocyte donation from someone, sperm donation, embryos donated and gestational surrogate. This involves gestational carrier of the couple's own embryo. Oocyte donation is performed win carious ovarian failure conditions in general. Anonymous donors are screened very well, and hopefully in a hormonally synchronized recipient. They will be maintained on hormones through the first trimester in most cases. Oocyte donation has a very good success; especially with the improvement of cryopreservation. In older women, for instance up to age 45, there is good success with this procedure. Beyond 45 yo, there is lack of sufficient data to support doing this. Gestational carrier is indicated when there is no uterus, uterine complications, substantial risk to patient due to other conditions, same sex male couples for instance. Fertility preservation is performed in instances of cancer treatment. These include oocyte, sperm and embryo cryopreservation. These are experimental, but are coming down the pipeline in the future. Fertility preservation is not a guarantee that it will work but the odds do go up proportionate to the number of eggs frozen; although this does decline the older you are. Preimplantation genetic screening: random chromosome abnormalities, or embryonic aneuploidy is screened for more often now. Age of the woman is an impact, although problems can be derived from sperm too, but there is no age impact in men. Embryos can be screened for such things as SC disease and CF. Now embryos are biopsied at day 5, and the trophectoderm (outer layer) is sampled for this. The embryo is the frozen after biopsy. These samples are sent to national labs for this. Usually, affected embryos will not be transferred. Next generation sequencing testing modalities are used commonly now. A significant proportion of anuploid embryos are found now, actually. It appears, for instance, that women who are of older gestational age, are to benefits most from genetic testing. Gestational carrier cases are also good reason for genetic testing, since the surrogate will be taking on a significant risk in carrying the child. CHAPTER 3: Evolving ART.  Major advances have been made in many areas. Cryopreservation, genetic testing and counseling, etc. We're not quite there yet with oocyte in-vitro maturation. Essentially embryonic stem cell therapy is still being done, but more adult stem cells are being used. Mitochondrial transfer therapy for mitochondrial diseases are being done in the U.K.  Germ line gene editing, for instance, to have HIV protection, has come up at a recent conference. This is in the very early stages of development though. Question from the audience: Pre-implantation genetic diagnosis - how many things can be screened for? PGS is still for a limited list of conditions, mostly the disease conditions are monogenic. Sex chromosome reports are also obtained. But, this is not done only for sex selection, especially in fertile couples. When appropriate, this can be chosen, however.  There is a slight chance of error, but certainty is very high. Other questions included freezing of eggs. Not only in dire circumstances such as cancer, but also for women who may want to wait to have children for any variety of reasons. Vitrification is the method used for this now which is essentially "flash freezing" and has been perfected over the past 7-8 years. Also, Dr. Damario mentions that studies show that visualized hydrosalpinx on U/S is associated with poorer IVF outcomes. Salpingectomy can improve chances with this. Cost is also addressed, and of course when insurance doesn't cover the cost of ART, there is a significant expense to the patient. Screening tests are commonly covered by insurance. Finally, Dr. Damario comments on a question regarding egg transfer. Transferring eggs to someone else is not a simple task and is governed by the FDA, much like transplant surgery.  

On this week's episode we speak with Valerie Landis.  Valerie is the host of the Eggology Club Podcast, writer of the Blog Eggsperience and nominee for a WEGO Health Award.  Valerie discusses her decision to undergo cryopreservation of own eggs, and why she believes it is a good option for many women.  

In an exclusive interview with TheRightDoctors on Emcure AICOG tv, Dr. Nimish Shelat, consultant at Newberg IVF, Gujarat Institute of reproductive Medicine, spoke on the topic of oocyte donation. This is what he said: ' Oocyte donation is a very very important topic. Ideal donors are those which are almost 21 years of age. They should not have any kind of disease particularly genetic disorder because genetic disorder then can be carried forward. College girls who want who make money they just step in our clinics, but we do not accept them because they are under lot of stress of studies at the same time we do not want that they do something later they will regret. It is very important to keep all the details of the donor because this is a very very important record you may be able to trace other issues later on. You should have complete profile including height, weight, health, respiratory and cardiovascular system assessment. She should not carry HIV infection. The parents who will later on the recipient parents who will be having the baby, may have a baby which carries a blood group of the donor also. So both have to be counseled.' TheRightDoctors, a Google Launchpad Digital Health StartUp, is one of the leaders in production and dissemination of Medical Information. We bring Insights from the World's Best Medical Minds to digital audiences worldwide across leading digital and social media platforms. Subscribe for more videos: https: https://goo.gl/BDq1gL Our Social Media Links : Facebook - https://goo.gl/YO1oel Google + - https://goo.gl/ImMXq5 Twitter - https://goo.gl/J1gtvw LinkedIn - https://goo.gl/FClkyl

How catastrophes help oocytes avoid disaster During meiosis, oocytes must attach homologous chromosomes to opposite spindle poles, but the cells take several hours to assemble a bipolar spindle. Gluszek et al. reveal that, in Drosophila oocytes, the microtubule catastrophe–promoting protein Sentin delays the formation of stable kinetochore–microtubule attachments until spindle assembly is complete, thereby preventing homologous chromosomes from incorrectly attaching to the same spindle pole. This biosights episode presents the paper by Głuszek et al. from the December 21st, 2015, issue of The Journal of Cell Biology and includes an interview with the paper's senior author, Hiroyuki Ohkura (University of Edinburgh, Edinburgh, Scotland, UK). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research. Subscribe to biosights via iTunes or RSS View biosights archive The Rockefeller University Press biosights@rockefeller.edu

One of the most critical periods in mammalian development is early embryogenesis. Its timing and morphology have been well studied, but molecular processes are still poorly understood. Several transcriptomic studies have addressed these molecular events. However, development of early embryonic stages before activation of the embryonic genome depends on sufficiently stored products of the maternal genome and adequate de/activation and relocation of proteins. Therefore, this thesis addresses early mammalian development, i.e., oocyte maturation and the first steps of embryogenesis, by holistic iTRAQ-based discovery approaches and by a targeted approach based on SRM. Numerous proteins important for oocyte maturation and embryogenesis so far not described in the mammalian system were identified and quantified, and protein profiles for key players previously described in the literature were contributed. In oocyte maturation, bioinformatics analysis revealed proteins increasing in abundance involved in rearrangement of the cytoskeleton, protein transport and cell cycle progression. Supplementation of oocyte maturation media with the gonadotropins follicle stimulation hormone and luteinizing hormone resulted in an increase of developmentally important proteins, but did not change developmental rates. Addition of growth hormone during oocyte maturation led to diverse and opposing effects at the level of proteins and also did not influence subsequent embryo development. During early embryogenesis, a considerable fraction of proteins continuously increased in abundance, despite a strongly attenuated rate of translation reported for this period. Bioinformatics analysis revealed particularly interesting proteins involved in the p53 pathway, lipid metabolism and mitosis. Activation of the unfolded protein response is demonstrated, which may be an indicator of the still lower success rates of in vitro versus in vivo embryo production. Relevant differences between transcript and protein abundance levels were detected, e.g., for WEE2, which highlights the importance of innovative proteomic tools and workflows to complement transcriptome data of early embryogenesis. iTRAQ results of early embryogenesis were successfully verified by targeted 5-plex SRM analysis. By principal component analysis, SRM quantifications comprising a panel of only five proteins were shown to discriminate between all four developmental stages analyzed. Using an expanded 27-plex SRM assay, proteins were quantified in nine developmentally interesting stages and absolute protein contents were established for nine proteins. SRM is a highly sensitive tool for detection of disturbances and disorders of embryonic development at the molecular level, thus complementing morphological analyses by high resolution microscopy.

In the in vitro production of embryos in humans and animals it is aimed to produce embryos of good quality in order to reach a high pregnancy rate after the transfer on a recipient. Nevertheless, data until 2007 show that in Europe the pregnancy rate after the transfer of human IVF embryos was only 33% (de Mouzon et al., 2012). Recently time-lapse imaging of early embryonic cleavage was found to be a helpful and non-invasive tool to predict the developmental capacity of embryos and select embryos of good quality (Wong et al., 2010, Sugimura et al., 2012). As the morphokinetic parameters of the first and second cleavage were the most predictive values, the fate of an embryo seems to be, at least partly, already determined very early in embryogenesis. This determination of the developmental competence might even go back further namely until oocyte maturation. In this experiment we used bovine embryos as an animal model to study the reasons for success and failure of mitotic cleavage during early embryo development. First a live monitoring system for the observation of bovine embryos was applied in order to search for the values that are highly predictable for the developmental competence to the blastocyst stage. To consider the effect of the oocyte maturation onto the further development, we observed embryos from oocytes after in vitro (n=398) versus in vivo maturation (n=143). In average embryos that developed to the blastocyst stage showed an earlier timing of the first, second and third cleavage than embryos that arrested (p

Ein bislang nur wenig verstandenes chemo¬sensorisches Zellsystem stellen Spermien dar, die im weib¬lichen Genital¬trakt komplexe Gemische ganz verschiedener Liganden wahr-nehmen müssen, um ihre für eine erfolgreiche Befruchtung essentiellen Aufgaben erfüllen zu können. Dazu gehören u. a. ein sekundärer Reifungsprozess (Kapazitierung), die Weg¬findung zur Eizelle im Eileiter und die Akrosom¬reaktion zur enzymatischen Auflösung der Glyko¬protein¬matrix (Zona pellucida) der Oocyte. Die Sensor¬moleküle auf der Oberfläche des Spermiums, die eine Erkennung bestehender Konzentrations-gradienten von Amino¬säuren, Kohlen¬hydraten, Hormonen, von verschiedensten Ionen und Protonen im luminalen Milieu des weiblichen Genital¬trakts sowie der Kohlenhydrat-reichen Zona pellucida ermöglichen, sind jedoch trotz ihrer Bedeutung für eine erfolgreiche Fertilisation weitgehend unbekannt. Geschmacks¬rezeptoren repräsentieren spezialisierte Erkennungs¬moleküle, die in Sinnes-zellen der Zunge die präzise Detektion eines breiten Spektrums chemisch sehr diverser Geschmacks¬stoffe ermöglichen, welche auffällige Ähnlichkeiten mit den potentiellen Liganden in der wässrigen Umgebung von Spermien im weiblichen Genital¬trakt aufweisen. Interessanterweise werden diese Rezeptorproteine aber nicht nur in Geschmacks¬sinneszellen, sondern auch in chemosensorischen Zellen einer Vielzahl extra-oraler Gewebe exprimiert. In der vorliegenden Arbeit wurde deshalb mit Hilfe biochemischer, molekular- und zell-biologischer Techniken sowie mit reproduktions¬biologischen Methoden und unter Verwendung Geschmacks¬rezeptor-defizienter Mäuse der Frage nachgegangen, ob Rezeptor¬moleküle des Geschmacks¬systems als Kandidaten für chemische Sensor-moleküle von Spermien in Betracht kommen. Dabei wurde ein Detektions¬molekül für saure Geschmacksstoffe, der PKD2L1, immun-cyto¬chemisch im Hoden der Maus und in reifen murinen Spermien nach¬gewiesen. Funktionell könnte dieser im Flagellum von Spermien exprimierte Ionenkanal an der Registrierung der verschiedenen Protonen¬konzentrationen im Milieu des weib¬lichen Genital¬trakts beteiligt sein. Weiterhin konnte eine Expression von gustatorischen GPCRs der Tas1r-Familie (süß/umami) und Tas2r-Familie (bitter), in männ¬lichen Reproduktions¬organen und in reifen Spermien gezeigt werden. Zudem wurden Hinweise auf die Expression der gustatorischen G Protein α Untereinheit Gustducin, die in Geschmacks¬sinnes¬zellen an der Signal¬transduktion dieser beiden Rezeptor¬familien beteiligt ist, im männlichen Reproduktions¬system erbracht. Im Einzelnen konnten mit der RT-PCR-Technik Transkripte von 28 der insgesamt 35 Mitglieder der großen Familie der murinen Bitter¬rezeptoren (Tas2rs) aus Hoden-gewebe amplifiziert werden. Die Bedeutung der Expression von Bitter¬rezeptoren für die Reproduktion wurde exemplarisch anhand einer Gen-defizienten Maus für den Tas2r131 unter¬sucht. Bei dieser Knockin-Maus¬linie war die kodierende Rezeptor¬sequenz durch eine GFP-Expressions¬kassette ersetzt worden, so dass das Maus¬modell gleich-zeitig auch eine Bestätigung der Expression des Tas2r131 in späten Keim¬zell¬stadien der Spermato¬genese ermöglichte. Bei der Fertilitätsanalyse Tas2r131-defizienter Tiere waren unter Labor-Zucht¬bedingungen keine Veränderungen in der Anzahl der Nach¬kommen pro Wurf oder der Zeitspanne zwischen den Würfen feststellbar. Allerdings wiesen Tas2r131-defiziente Männ¬chen signifikant mehr epididymale Spermien auf als Wildtyp-Tiere. Darüber hinaus war bei Verpaarungs¬studien mit hetero¬zygoten Männchen eine Genotyp-Verschiebung zugunsten des Tas2r131 [-] Allels zu registrieren. Dieser Phänotyp könnte darauf hindeuten, dass der Tas2r131 eine funktionelle Rolle bei Tas2r-abhängigen Auswahlprozessen verschiedener Spermien¬populationen spielt, bei denen sich z. B. durch eine Regulation der Apoptose im Verlauf der Keimzell¬bildung (Spermatogenese) oder auch durch eine Beeinflussung z. B. der Weg¬findung im weiblichen Genitaltrakt ein Selektions¬vorteil für Tas2r131-defiziente Spermien ergeben könnte. Aus der Familie der Tas1-Rezeptoren, deren drei Mitglieder als Heterodimere für die Erkennung von süßen Stimuli und dem Geschmack von Mononatrium¬glutamat („umami“) verant¬wort¬lich sind, konnten in RT-PCR-Experimenten die beiden Unter-einheiten des Umami-Rezeptors, der Tas1r1 und Tas1r3, aus Hodengewebe der Maus amplifiziert werden. Mit Hilfe Subtyp- und Spezies-spezifischer Antikörper konnte gezeigt werden, dass beide Rezeptor¬proteine im Akrosom und in distinkten Abschnitten des Flagellums von murinen und humanen Spermien exprimiert werden. Die funktionelle Rolle des Umami-Rezeptors wurde mit Hilfe einer Tas1r1-defizienten mCherry Reportermauslinie unter¬sucht, die unter optimalen Zuchtbedingungen ebenfalls keine Fertilitäts¬einschränkungen erkennen ließ. Im Hoden dieser Tas1r1-defizienten Tiere waren jedoch morpho¬logische Veränderungen des Keim¬epithels und eine signifikant erhöhte Apoptose¬rate zu registrieren, die allerdings keine verminderte Anzahl reifer Spermien oder Störungen der Morphologie oder Motilität dieser Zellen zur Folge hatte. Stimulierungsexperimente mit isolierter Zona pellucida, dem physiologischen Auslöser der Akrosomreaktion, haben zudem gezeigt, dass keine Ein¬schränkungen bei Spermien Tas1r1-defizienter Tiere fest¬zustellen waren. Allerdings wiesen Tas1r1-defiziente Spermien eine signifikant höhere Rate an spontaner Akrosom¬reaktion auf, die in unkapazitierten Zellen mit signifikant erhöhten basalen Konzentrationen der second messenger cAMP und Ca2+ einherging. Durch eine Reduzierung der intra¬zellulären Konzentrationen dieser Botenstoffe, die elementare Aufgaben des Spermiums im Verlauf des sequentiellen Prozesses der Fertilisation regulieren, könnten Tas1-Rezeptoren somit durch eine basale Rezeptor-aktivität oder durch eine Liganden-induzierte Rezeptor¬stimulation sicherstellen, dass Spermien im weiblichen Genitaltrakt in einem Ruhezustand erhalten werden, bevor sie in Kontakt mit der Eizelle kommen können. Insgesamt kann dieser Nachweis einer funktionellen Expression von Geschmacks-rezeptoren in Spermien zu einem besseren Verständnis der Regulations¬mechanismen zentraler Spermien¬funktionen beitragen und langfristig möglicherweise auch repro-duktions¬medizinische Perspektiven zur gezielten positiven bzw. negativen Manipulation von Spermien und damit zur Behandlung männlicher Infertilität bzw. zur Entwicklung nicht-hormoneller Verhütungsmittel für den Mann eröffnen.

Several mRNAs are specifically transported to the anterior and posterior regions of Drosophila oocytes by microtubule-based motor proteins, but the organization of microtubules in these cells is unclear. Parton et al.  reveal that oocyte microtubules are highly dynamic and display a PAR-1-dependent bias in polarity that facilitates transport of oskar mRNA to the oocyte posterior. This biosights episode presents the paper by Parton et al. from the July 11, 2011, issue of The Journal of Cell Biology, and includes an interview with authors Richard Parton and Ilan Davis (University of Oxford, UK). Produced by Caitlin Sedwick and Ben Short. Subscribe to biosights via iTunes or RSS View biosights archive The Rockefeller University Press biosights@rockefeller.edu

Similarities between the bovine female and women in terms of reproduction and fertility, such as oogenesis, folliculogenesis and reduced fertility with advanced age make the bovine a valuable model for the study of ovarian function and dysfunction as well as reproductive aging in women. The aim of the present work was to investigate the influence of donor age on follicle numbers, yield and quality of COCs obtained by repeated OPU and on the developmental competence in vitro after oocyte maturation in vitro versus in vivo. Further, the ability of oocytes from different age classes to reprogram nuclei of bovine fetal fibroblasts was studied. Since the individual is a major factor influencing parameters of fertility and results of ART in both humans and cattle, the present study used in parts the same animals to rule out inter-individual effects on the response to one or the other approach. Experiment 1 investigated the effect of donor age in non-superstimulated German Simmental heifers (n = 12, 14 months at the beginning of the experiments), young cows in their first lactation (n = 8, 2-4 years) and old cows (n = 8, 10-15 years). A total of 38 OPU sessions were performed in two experimental periods on independent sets of animals from all age classes: 5/5/5 (32 sessions) and 7/3/3 (6 sessions). In spite of a marked influence of the experimental period, a number of parameters were also significantly affected by donor age. The total number of follicles increased with age (P

Guest: Eric D. Levens, MD Host: Maurice Pickard, MD Dr. Eric Levens, Lieutenant Commander in the United States Public Health Services, discusses ethical issues that arise when research involves human oocyte donation. Among the topics discussed by Dr. Levens and host Dr. Maurice Pickard are issues surrounding donor protection. There are tensions around the potential greater risks than benefits to the donor. Additional controversies exist around the issues of informed consent and compensation.